LOCUS       DAFCLG010000001        91976 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFCLG010000001 DAFCLG010000000
VERSION     DAFCLG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.001"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            18..535
                     /locus_tag="LND56_000001"
                     /pseudo
     CDS             18..535
                     /locus_tag="LND56_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            663..1346
                     /locus_tag="LND56_000002"
     CDS             663..1346
                     /locus_tag="LND56_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBK6741960.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="LND56_000003"
     tRNA            1655..1728
                     /locus_tag="LND56_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1887..2246
                     /locus_tag="LND56_000004"
     CDS             1887..2246
                     /locus_tag="LND56_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBK6741961.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2369..4000
                     /locus_tag="LND56_000005"
     CDS             2369..4000
                     /locus_tag="LND56_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBK6741962.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4062..4703)
                     /locus_tag="LND56_000006"
     CDS             complement(4062..4703)
                     /locus_tag="LND56_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6741963.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4889..4965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4985..5293
                     /gene="rplU"
                     /locus_tag="LND56_000007"
     CDS             4985..5293
                     /gene="rplU"
                     /locus_tag="LND56_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK6741964.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5320..5664
                     /locus_tag="LND56_000008"
     CDS             5320..5664
                     /locus_tag="LND56_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBK6741965.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5677..5970
                     /gene="rpmA"
                     /locus_tag="LND56_000009"
     CDS             5677..5970
                     /gene="rpmA"
                     /locus_tag="LND56_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK6741966.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6404..9211
                     /locus_tag="LND56_000010"
     CDS             6404..9211
                     /locus_tag="LND56_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6741967.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9402..9893
                     /locus_tag="LND56_000011"
     CDS             9402..9893
                     /locus_tag="LND56_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6741968.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9996..10985
                     /gene="manX"
                     /locus_tag="LND56_000012"
     CDS             9996..10985
                     /gene="manX"
                     /locus_tag="LND56_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK6741969.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11018..11821
                     /locus_tag="LND56_000013"
     CDS             11018..11821
                     /locus_tag="LND56_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBK6741970.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11842..12756
                     /locus_tag="LND56_000014"
     CDS             11842..12756
                     /locus_tag="LND56_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK6741971.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12839..13213
                     /locus_tag="LND56_000015"
     CDS             12839..13213
                     /locus_tag="LND56_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBK6741972.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13352..13597
                     /locus_tag="LND56_000016"
     CDS             13352..13597
                     /locus_tag="LND56_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6741973.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13769..14833
                     /locus_tag="LND56_000017"
     CDS             13769..14833
                     /locus_tag="LND56_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6741974.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15079..15504
                     /locus_tag="LND56_000018"
     CDS             15079..15504
                     /locus_tag="LND56_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6741975.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15497..15946
                     /gene="nusB"
                     /locus_tag="LND56_000019"
     CDS             15497..15946
                     /gene="nusB"
                     /locus_tag="LND56_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK6741976.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16043..16897
                     /locus_tag="LND56_000020"
     CDS             16043..16897
                     /locus_tag="LND56_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBK6741977.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16898..18235
                     /locus_tag="LND56_000021"
     CDS             16898..18235
                     /locus_tag="LND56_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK6741978.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18240..18464
                     /locus_tag="LND56_000022"
     CDS             18240..18464
                     /locus_tag="LND56_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK6741979.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18466..19356
                     /locus_tag="LND56_000023"
     CDS             18466..19356
                     /locus_tag="LND56_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6741980.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19365..20183
                     /locus_tag="LND56_000024"
     CDS             19365..20183
                     /locus_tag="LND56_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBK6741981.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20290..20739
                     /locus_tag="LND56_000025"
     CDS             20290..20739
                     /locus_tag="LND56_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6741982.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20757..22439
                     /gene="recN"
                     /locus_tag="LND56_000026"
     CDS             20757..22439
                     /gene="recN"
                     /locus_tag="LND56_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK6741983.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22494..22610)
                     /locus_tag="LND56_000027"
     CDS             complement(22494..22610)
                     /locus_tag="LND56_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBK6741984.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22683..23639)
                     /locus_tag="LND56_000028"
     CDS             complement(22683..23639)
                     /locus_tag="LND56_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6741985.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23810..24178
                     /locus_tag="LND56_000029"
     CDS             23810..24178
                     /locus_tag="LND56_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBK6741986.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24348..24779
                     /gene="mraZ"
                     /locus_tag="LND56_000030"
     CDS             24348..24779
                     /gene="mraZ"
                     /locus_tag="LND56_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK6741987.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24800..25759
                     /gene="rsmH"
                     /locus_tag="LND56_000031"
     CDS             24800..25759
                     /gene="rsmH"
                     /locus_tag="LND56_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK6741988.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25764..26165
                     /gene="ftsL"
                     /locus_tag="LND56_000032"
     CDS             25764..26165
                     /gene="ftsL"
                     /locus_tag="LND56_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK6741989.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26166..28358
                     /locus_tag="LND56_000033"
     CDS             26166..28358
                     /locus_tag="LND56_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6741990.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28384..29346
                     /locus_tag="LND56_000034"
     CDS             28384..29346
                     /locus_tag="LND56_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK6741991.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29360..30721
                     /locus_tag="LND56_000035"
     CDS             29360..30721
                     /locus_tag="LND56_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK6741992.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30747..31835
                     /gene="murG"
                     /locus_tag="LND56_000036"
     CDS             30747..31835
                     /gene="murG"
                     /locus_tag="LND56_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK6741993.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31850..33070
                     /locus_tag="LND56_000037"
     CDS             31850..33070
                     /locus_tag="LND56_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBK6741994.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33226..34551
                     /gene="ftsA"
                     /locus_tag="LND56_000038"
     CDS             33226..34551
                     /gene="ftsA"
                     /locus_tag="LND56_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK6741995.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34574..35815
                     /gene="ftsZ"
                     /locus_tag="LND56_000039"
     CDS             34574..35815
                     /gene="ftsZ"
                     /locus_tag="LND56_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK6741996.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35832..36509
                     /locus_tag="LND56_000040"
     CDS             35832..36509
                     /locus_tag="LND56_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBK6741997.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36523..37122
                     /locus_tag="LND56_000041"
     CDS             36523..37122
                     /locus_tag="LND56_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBK6741998.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37137..37424
                     /locus_tag="LND56_000042"
     CDS             37137..37424
                     /locus_tag="LND56_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBK6741999.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37473..38255
                     /locus_tag="LND56_000043"
     CDS             37473..38255
                     /locus_tag="LND56_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBK6742000.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38276..38983
                     /locus_tag="LND56_000044"
     CDS             38276..38983
                     /locus_tag="LND56_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBK6742001.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39245..42043
                     /gene="ileS"
                     /locus_tag="LND56_000045"
     CDS             39245..42043
                     /gene="ileS"
                     /locus_tag="LND56_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK6742002.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42092..43618)
                     /locus_tag="LND56_000046"
     CDS             complement(42092..43618)
                     /locus_tag="LND56_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK6742003.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43633..44280)
                     /locus_tag="LND56_000047"
     CDS             complement(43633..44280)
                     /locus_tag="LND56_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBK6742004.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44464..44793)
                     /locus_tag="LND56_000048"
     CDS             complement(44464..44793)
                     /locus_tag="LND56_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBK6742005.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44970..45698
                     /gene="sfsA"
                     /locus_tag="LND56_000049"
     CDS             44970..45698
                     /gene="sfsA"
                     /locus_tag="LND56_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK6742006.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45714..46727
                     /locus_tag="LND56_000050"
     CDS             45714..46727
                     /locus_tag="LND56_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6742007.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46727..48004
                     /locus_tag="LND56_000051"
     CDS             46727..48004
                     /locus_tag="LND56_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6742008.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48067..50769
                     /locus_tag="LND56_000052"
     CDS             48067..50769
                     /locus_tag="LND56_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6742009.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50921..51238
                     /locus_tag="LND56_000053"
     CDS             50921..51238
                     /locus_tag="LND56_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6742010.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51268..51588
                     /locus_tag="LND56_000054"
     CDS             51268..51588
                     /locus_tag="LND56_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6742011.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51696..53156
                     /locus_tag="LND56_000055"
     CDS             51696..53156
                     /locus_tag="LND56_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6742012.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53224..53445
                     /locus_tag="LND56_000056"
     CDS             53224..53445
                     /locus_tag="LND56_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742013.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53476..53658
                     /locus_tag="LND56_000057"
     CDS             53476..53658
                     /locus_tag="LND56_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBK6742014.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53658..54071
                     /locus_tag="LND56_000058"
     CDS             53658..54071
                     /locus_tag="LND56_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBK6742015.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54194..55375
                     /locus_tag="LND56_000059"
     CDS             54194..55375
                     /locus_tag="LND56_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742016.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55446..55610)
                     /locus_tag="LND56_000060"
     CDS             complement(55446..55610)
                     /locus_tag="LND56_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742017.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55764..55837
                     /locus_tag="LND56_000061"
     tRNA            55764..55837
                     /locus_tag="LND56_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55798..55800,aa:Arg,seq:cct)
     gene            complement(55906..57045)
                     /locus_tag="LND56_000062"
     CDS             complement(55906..57045)
                     /locus_tag="LND56_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6742018.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57344..57979)
                     /locus_tag="LND56_000063"
     CDS             complement(57344..57979)
                     /locus_tag="LND56_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742019.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58092..58727)
                     /locus_tag="LND56_000064"
     CDS             complement(58092..58727)
                     /locus_tag="LND56_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBK6742020.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58761..59222)
                     /locus_tag="LND56_000065"
     CDS             complement(58761..59222)
                     /locus_tag="LND56_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBK6742021.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59352..59783)
                     /locus_tag="LND56_000066"
     CDS             complement(59352..59783)
                     /locus_tag="LND56_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6742022.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59801..60121)
                     /locus_tag="LND56_000067"
     CDS             complement(59801..60121)
                     /locus_tag="LND56_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6742023.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60420..61196
                     /locus_tag="LND56_000068"
     CDS             60420..61196
                     /locus_tag="LND56_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK6742024.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61211..61414
                     /locus_tag="LND56_000069"
     CDS             61211..61414
                     /locus_tag="LND56_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742025.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61430..61768
                     /locus_tag="LND56_000070"
     CDS             61430..61768
                     /locus_tag="LND56_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBK6742026.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADNTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61755..61934
                     /locus_tag="LND56_000071"
     CDS             61755..61934
                     /locus_tag="LND56_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742027.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(61977..62447)
                     /locus_tag="LND56_000072"
     CDS             complement(61977..62447)
                     /locus_tag="LND56_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742028.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62534..63232
                     /locus_tag="LND56_000073"
     CDS             62534..63232
                     /locus_tag="LND56_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK6742029.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63410..63751
                     /locus_tag="LND56_000074"
     CDS             63410..63751
                     /locus_tag="LND56_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742030.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63744..64415
                     /locus_tag="LND56_000075"
     CDS             63744..64415
                     /locus_tag="LND56_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBK6742031.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64421..65107
                     /locus_tag="LND56_000076"
     CDS             64421..65107
                     /locus_tag="LND56_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742032.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            65110..65859
                     /locus_tag="LND56_000077"
     CDS             65110..65859
                     /locus_tag="LND56_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742033.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65871..66140
                     /locus_tag="LND56_000078"
     CDS             65871..66140
                     /locus_tag="LND56_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742034.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            66145..66309
                     /locus_tag="LND56_000079"
     CDS             66145..66309
                     /locus_tag="LND56_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742035.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66302..66604
                     /locus_tag="LND56_000080"
     CDS             66302..66604
                     /locus_tag="LND56_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742036.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            66601..66762
                     /locus_tag="LND56_000081"
     CDS             66601..66762
                     /locus_tag="LND56_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6742037.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            66759..67064
                     /locus_tag="LND56_000082"
     CDS             66759..67064
                     /locus_tag="LND56_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742038.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            67064..67420
                     /locus_tag="LND56_000083"
     CDS             67064..67420
                     /locus_tag="LND56_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBK6742039.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            67407..67625
                     /locus_tag="LND56_000084"
     CDS             67407..67625
                     /locus_tag="LND56_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742040.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            67622..68041
                     /locus_tag="LND56_000085"
     CDS             67622..68041
                     /locus_tag="LND56_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBK6742041.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            68038..68595
                     /locus_tag="LND56_000086"
     CDS             68038..68595
                     /locus_tag="LND56_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK6742042.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            68592..68888
                     /locus_tag="LND56_000087"
     CDS             68592..68888
                     /locus_tag="LND56_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742043.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            68965..69378
                     /locus_tag="LND56_000088"
     CDS             68965..69378
                     /locus_tag="LND56_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK6742044.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            69687..69839
                     /locus_tag="LND56_000089"
     CDS             69687..69839
                     /locus_tag="LND56_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742045.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(69836..70111)
                     /locus_tag="LND56_000090"
     CDS             complement(69836..70111)
                     /locus_tag="LND56_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742046.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            70565..70771
                     /locus_tag="LND56_000091"
     CDS             70565..70771
                     /locus_tag="LND56_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742047.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            70967..71134
                     /locus_tag="LND56_000092"
     CDS             70967..71134
                     /locus_tag="LND56_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK6742048.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            71160..71504
                     /locus_tag="LND56_000093"
     CDS             71160..71504
                     /locus_tag="LND56_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6742049.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            71509..71790
                     /locus_tag="LND56_000094"
     CDS             71509..71790
                     /locus_tag="LND56_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742050.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            71893..72207
                     /locus_tag="LND56_000095"
     CDS             71893..72207
                     /locus_tag="LND56_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK6742051.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            72185..73879
                     /locus_tag="LND56_000096"
     CDS             72185..73879
                     /locus_tag="LND56_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6742052.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            73899..75077
                     /locus_tag="LND56_000097"
     CDS             73899..75077
                     /locus_tag="LND56_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6742053.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            75040..75726
                     /locus_tag="LND56_000098"
     CDS             75040..75726
                     /locus_tag="LND56_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK6742054.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            75726..76886
                     /locus_tag="LND56_000099"
     CDS             75726..76886
                     /locus_tag="LND56_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6742055.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            76896..77771
                     /locus_tag="LND56_000100"
     CDS             76896..77771
                     /locus_tag="LND56_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742056.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            77768..78079
                     /locus_tag="LND56_000101"
     CDS             77768..78079
                     /locus_tag="LND56_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742057.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            78069..78422
                     /locus_tag="LND56_000102"
     CDS             78069..78422
                     /locus_tag="LND56_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6742058.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            78412..78813
                     /locus_tag="LND56_000103"
     CDS             78412..78813
                     /locus_tag="LND56_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742059.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            78806..79210
                     /locus_tag="LND56_000104"
     CDS             78806..79210
                     /locus_tag="LND56_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742060.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            79222..79830
                     /locus_tag="LND56_000105"
     CDS             79222..79830
                     /locus_tag="LND56_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6742061.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            79850..80212
                     /locus_tag="LND56_000106"
     CDS             79850..80212
                     /locus_tag="LND56_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742062.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            80414..83845
                     /locus_tag="LND56_000107"
                     /pseudo
     CDS             80414..83845
                     /locus_tag="LND56_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            83896..84633
                     /locus_tag="LND56_000108"
     CDS             83896..84633
                     /locus_tag="LND56_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6742063.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            84643..86934
                     /locus_tag="LND56_000109"
     CDS             84643..86934
                     /locus_tag="LND56_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6742064.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            86958..89084
                     /locus_tag="LND56_000110"
     CDS             86958..89084
                     /locus_tag="LND56_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742065.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            89101..89250
                     /locus_tag="LND56_000111"
     CDS             89101..89250
                     /locus_tag="LND56_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742066.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            89247..89693
                     /locus_tag="LND56_000112"
     CDS             89247..89693
                     /locus_tag="LND56_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742067.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            89695..89832
                     /locus_tag="LND56_000113"
     CDS             89695..89832
                     /locus_tag="LND56_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK6742068.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            89870..90163
                     /locus_tag="LND56_000114"
     CDS             89870..90163
                     /locus_tag="LND56_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742069.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            90160..90384
                     /locus_tag="LND56_000115"
     CDS             90160..90384
                     /locus_tag="LND56_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6742070.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            90381..91406
                     /locus_tag="LND56_000116"
     CDS             90381..91406
                     /locus_tag="LND56_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6742071.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            91596..91669
                     /locus_tag="LND56_000117"
     tRNA            91596..91669
                     /locus_tag="LND56_000117"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:91630..91632,aa:Ile,seq:cat)
BASE COUNT        31084 a        15006 c        19341 g        26545 t
ORIGIN      
        1 gaaagaagtt gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt
       61 tgattatatt tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga
      121 ctccttatat tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat
      181 atgtacgacc gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa
      241 attcaggaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1861 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1921 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1981 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2041 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2101 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2161 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2221 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2281 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2341 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2401 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2461 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2521 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2581 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2641 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2701 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2761 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2821 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2881 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2941 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3001 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3061 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3121 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3181 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3241 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3301 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3361 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3421 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3481 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3541 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3601 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3661 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3721 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3781 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3841 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3901 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3961 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4021 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4081 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4141 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4201 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4261 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4321 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4381 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4441 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4501 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4561 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4621 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4681 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4741 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4801 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4861 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4921 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4981 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5041 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5101 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5161 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5221 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5281 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5341 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5401 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5461 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5521 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5581 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5641 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5701 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5761 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5821 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5881 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5941 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6001 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6061 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6121 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6181 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6241 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6301 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6361 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6421 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6481 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6541 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6601 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6661 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6721 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6781 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     6841 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     6901 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     6961 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7021 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7081 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7141 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7201 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7261 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7321 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7381 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7441 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7501 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7561 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7621 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7681 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7741 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7801 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     7861 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     7921 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     7981 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8041 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8101 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8161 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8221 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8281 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8341 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8401 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8461 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8521 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8581 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8641 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8701 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8761 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     8821 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     8881 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     8941 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9001 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9061 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9121 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9181 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9241 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9301 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9361 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9421 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9481 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9541 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9601 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9661 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9721 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9781 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     9841 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     9901 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     9961 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10021 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10081 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10141 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10201 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10261 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10321 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10381 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10441 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10501 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10561 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10621 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10681 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10741 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10801 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    10861 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    10921 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    10981 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11041 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11101 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11161 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11221 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11281 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11341 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11401 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11461 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11521 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11581 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11641 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11701 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11761 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    11821 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    11881 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    11941 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12001 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12061 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12121 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12181 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12241 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12301 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12361 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12421 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12481 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12541 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12601 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12661 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12721 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12781 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    12841 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    12901 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    12961 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13021 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13081 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13141 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13201 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13261 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13321 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13381 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13441 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13501 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13561 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13621 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    13681 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    13741 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    13801 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    13861 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    13921 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    13981 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    14041 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    14101 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    14161 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    14221 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    14281 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    14341 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    14401 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    14461 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    14521 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    14581 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    14641 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    14701 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    14761 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    14821 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    14881 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    14941 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    15001 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    15061 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    15121 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    15181 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    15241 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    15301 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    15361 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    15421 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    15481 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    15541 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    15601 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    15661 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    15721 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    15781 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    15841 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    15901 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    15961 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    16021 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    16081 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    16141 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    16201 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    16261 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    16321 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    16381 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    16441 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    16501 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    16561 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    16621 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    16681 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    16741 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    16801 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    16861 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    16921 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    16981 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    17041 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    17101 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    17161 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    17221 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    17281 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    17341 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    17401 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    17461 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    17521 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    17581 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    17641 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    17701 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    17761 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    17821 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    17881 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    17941 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    18001 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    18061 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    18121 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    18181 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    18241 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    18301 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    18361 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    18421 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    18481 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    18541 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    18601 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    18661 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    18721 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    18781 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    18841 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    18901 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    18961 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    19021 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    19081 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    19141 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    19201 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    19261 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    19321 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    19381 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    19441 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    19501 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    19561 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    19621 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    19681 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    19741 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    19801 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    19861 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    19921 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    19981 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    20041 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    20101 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    20161 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    20221 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    20281 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    20341 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    20401 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    20461 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    20521 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    20581 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    20641 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    20701 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    20761 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    20821 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    20881 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    20941 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    21001 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    21061 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    21121 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    21181 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    21241 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    21301 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    21361 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    21421 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    21481 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    21541 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    21601 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    21661 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    21721 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    21781 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    21841 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    21901 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    21961 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    22021 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    22081 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    22141 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    22201 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    22261 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    22321 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    22381 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    22441 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    22501 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    22561 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    22621 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    22681 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    22741 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    22801 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    22861 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    22921 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    22981 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    23041 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    23101 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    23161 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    23221 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    23281 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    23341 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    23401 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    23461 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    23521 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    23581 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    23641 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    23701 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    23761 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    23821 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    23881 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    23941 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    24001 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    24061 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    24121 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    24181 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    24241 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    24301 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    24361 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    24421 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    24481 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    24541 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    24601 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    24661 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    24721 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    24781 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    24841 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    24901 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    24961 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    25021 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    25081 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    25141 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    25201 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    25261 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    25321 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    25381 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    25441 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    25501 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    25561 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    25621 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    25681 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    25741 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    25801 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    25861 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    25921 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    25981 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    26041 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    26101 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaggtga
    26161 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    26221 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    26281 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    26341 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    26401 tttatgatcg taacggtgtg gcgctcgcgg aagatgcctc ctcttattca attaaagcga
    26461 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    26521 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    26581 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    26641 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    26701 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    26761 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    26821 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    26881 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    26941 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    27001 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    27061 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    27121 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    27181 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    27241 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    27301 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    27361 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    27421 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    27481 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    27541 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    27601 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    27661 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    27721 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaccggaa
    27781 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    27841 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    27901 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    27961 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    28021 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    28081 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    28141 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    28201 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    28261 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    28321 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    28381 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    28441 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    28501 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    28561 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    28621 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    28681 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    28741 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    28801 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    28861 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    28921 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    28981 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    29041 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    29101 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    29161 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    29221 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    29281 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    29341 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    29401 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    29461 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    29521 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    29581 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    29641 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    29701 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    29761 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    29821 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    29881 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    29941 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    30001 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    30061 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    30121 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    30181 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    30241 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    30301 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    30361 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    30421 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    30481 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    30541 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    30601 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    30661 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    30721 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    30781 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    30841 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    30901 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    30961 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    31021 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    31081 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    31141 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    31201 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    31261 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    31321 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    31381 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    31441 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    31501 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    31561 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    31621 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    31681 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    31741 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    31801 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    31861 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    31921 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    31981 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    32041 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    32101 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    32161 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    32221 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    32281 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    32341 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    32401 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    32461 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    32521 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    32581 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    32641 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    32701 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    32761 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    32821 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    32881 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    32941 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    33001 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    33061 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    33121 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    33181 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    33241 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    33301 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    33361 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    33421 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    33481 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    33541 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    33601 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    33661 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    33721 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    33781 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    33841 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    33901 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    33961 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    34021 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    34081 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    34141 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    34201 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    34261 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    34321 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    34381 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    34441 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    34501 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    34561 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    34621 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    34681 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    34741 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    34801 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    34861 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    34921 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    34981 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    35041 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    35101 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    35161 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    35221 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    35281 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    35341 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    35401 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    35461 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    35521 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    35581 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    35641 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    35701 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    35761 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    35821 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    35881 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    35941 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    36001 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    36061 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    36121 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    36181 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    36241 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    36301 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    36361 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    36421 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    36481 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    36541 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    36601 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    36661 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    36721 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    36781 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    36841 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    36901 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    36961 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    37021 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    37081 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    37141 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    37201 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    37261 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    37321 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    37381 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    37441 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    37501 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    37561 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    37621 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    37681 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    37741 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    37801 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    37861 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    37921 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    37981 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    38041 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    38101 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    38161 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    38221 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    38281 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    38341 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    38401 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    38461 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    38521 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    38581 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    38641 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    38701 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    38761 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    38821 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    38881 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    38941 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    39001 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    39061 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    39121 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    39181 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    39241 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    39301 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    39361 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    39421 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    39481 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    39541 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    39601 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    39661 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    39721 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    39781 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    39841 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    39901 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    39961 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    40021 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    40081 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    40141 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    40201 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    40261 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    40321 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    40381 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    40441 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    40501 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    40561 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    40621 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    40681 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    40741 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    40801 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    40861 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    40921 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    40981 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    41041 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    41101 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    41161 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    41221 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    41281 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    41341 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    41401 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    41461 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    41521 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    41581 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    41641 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    41701 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    41761 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    41821 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    41881 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    41941 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    42001 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    42061 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    42121 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    42181 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    42241 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    42301 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    42361 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    42421 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    42481 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    42541 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    42601 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    42661 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    42721 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    42781 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    42841 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    42901 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    42961 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    43021 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    43081 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    43141 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    43201 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    43261 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    43321 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    43381 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    43441 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    43501 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    43561 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    43621 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    43681 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    43741 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    43801 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    43861 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    43921 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    43981 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    44041 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    44101 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    44161 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    44221 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    44281 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    44341 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    44401 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    44461 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    44521 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    44581 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    44641 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    44701 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    44761 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    44821 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    44881 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    44941 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    45001 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    45061 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    45121 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    45181 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    45241 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    45301 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    45361 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    45421 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    45481 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    45541 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    45601 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    45661 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    45721 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    45781 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    45841 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    45901 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    45961 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    46021 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    46081 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    46141 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    46201 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    46261 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    46321 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    46381 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    46441 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    46501 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    46561 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    46621 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    46681 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    46741 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    46801 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    46861 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    46921 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    46981 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    47041 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    47101 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    47161 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    47221 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    47281 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    47341 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    47401 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    47461 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    47521 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    47581 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    47641 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    47701 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    47761 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    47821 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    47881 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    47941 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    48001 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    48061 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    48121 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    48181 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    48241 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    48301 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    48361 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    48421 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    48481 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    48541 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    48601 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    48661 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    48721 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    48781 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    48841 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    48901 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    48961 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    49021 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    49081 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    49141 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    49201 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    49261 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    49321 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    49381 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    49441 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    49501 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    49561 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    49621 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    49681 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    49741 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    49801 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    49861 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    49921 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    49981 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    50041 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    50101 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    50161 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    50221 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    50281 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    50341 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    50401 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    50461 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    50521 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    50581 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    50641 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    50701 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    50761 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    50821 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    50881 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    50941 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    51001 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    51061 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    51121 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    51181 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    51241 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    51301 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    51361 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    51421 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    51481 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    51541 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    51601 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    51661 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    51721 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    51781 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    51841 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    51901 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    51961 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    52021 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    52081 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    52141 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    52201 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    52261 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    52321 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    52381 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    52441 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    52501 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    52561 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    52621 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    52681 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    52741 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    52801 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    52861 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    52921 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    52981 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    53041 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    53101 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    53161 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    53221 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    53281 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    53341 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    53401 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    53461 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    53521 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    53581 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    53641 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    53701 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    53761 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    53821 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    53881 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    53941 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    54001 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    54061 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    54121 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    54181 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    54241 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    54301 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    54361 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    54421 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    54481 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    54541 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    54601 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    54661 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    54721 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    54781 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    54841 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    54901 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    54961 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    55021 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    55081 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    55141 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    55201 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    55261 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    55321 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    55381 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    55441 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    55501 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    55561 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    55621 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    55681 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    55741 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    55801 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    55861 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    55921 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    55981 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    56041 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    56101 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    56161 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    56221 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    56281 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    56341 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    56401 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    56461 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    56521 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    56581 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    56641 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    56701 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    56761 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    56821 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    56881 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    56941 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    57001 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    57061 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    57121 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    57181 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    57241 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    57301 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    57361 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    57421 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    57481 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    57541 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    57601 caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct
    57661 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    57721 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    57781 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    57841 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    57901 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    57961 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    58021 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    58081 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    58141 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    58201 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    58261 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    58321 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    58381 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    58441 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    58501 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    58561 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    58621 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
    58681 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
    58741 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
    58801 tcttgattaa taatcccttc atctaacaac tctttatact ttttaatttc atctgcactt
    58861 gaaatttgtg tttctttgga attattatct aatatagatt gttgcttctc tatatatttt
    58921 tttaaatttt ccatttcatt ccaatatttt tttgtaaaca taacggtgtt ttcatctttc
    58981 gtggccgcta atatacctcc gcgactttcg tttccgccta gcaaggtaaa ttgtatataa
    59041 ccattactca tacctggttt ttttaattgt acggctgata tatttttaaa ggggatagtt
    59101 ttttctcctt taataccttg attaactaaa tttataaatc cttttctaat aattgaaata
    59161 gaatcatcag ttactttaat caaggttttt cctggagatt ttattaaaat ctctttttcc
    59221 attttttctt cctcatttct gtgatatgat tttttttgta gaagctcata tatgaggaaa
    59281 gagcccgtgt tgcagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat
    59341 tcttgatgta ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg
    59401 cagacagcat aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt
    59461 tcgagattaa actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt
    59521 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
    59581 tacaaaactg aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga
    59641 aaggcttctg cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga
    59701 tagtagaaac caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac
    59761 atttctttca atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag
    59821 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
    59881 aaaaaacagt atcgtcttct gataaatcca catgtttagg ggtttctctt tcttctctac
    59941 ctagaaggta gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat
    60001 taggagtatt atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta
    60061 actgccgtat agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca
    60121 tcgataaaac aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa
    60181 tttgactaat tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa
    60241 agacaacaaa aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa
    60301 tgtttttatg tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat
    60361 tgaatttaga aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata
    60421 tgaacacacc gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg
    60481 atgaaccata ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag
    60541 atgctttgtc gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag
    60601 gtcaatcaag agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat
    60661 caaaacttcc ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa
    60721 ttagaaaaca tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag
    60781 atacaatcat tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag
    60841 aacaaaagat tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta
    60901 cagattcagt aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga
    60961 ataaattact tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca
    61021 aaaaacacat gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg
    61081 gtggcactaa aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg
    61141 aattactaaa aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag
    61201 gagttttagt atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga
    61261 atatgtcgaa agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag
    61321 caaaagctac ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat
    61381 tatagattcg attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct
    61441 agaagcaaaa atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga
    61501 gttaaaaaaa gctgataata ctggtagatg gatgacgttg ccagaagtgc tagaacggat
    61561 taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat
    61621 tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac
    61681 atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt
    61741 aaggaggcaa gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca
    61801 tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg
    61861 tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg
    61921 agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag
    61981 tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg
    62041 tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta
    62101 aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata
    62161 tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt
    62221 aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa
    62281 ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata
    62341 tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt
    62401 tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt
    62461 gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag
    62521 gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag
    62581 cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc
    62641 actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc
    62701 atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat
    62761 ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga
    62821 aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta
    62881 tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg
    62941 aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt
    63001 tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt
    63061 ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag
    63121 attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga
    63181 aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag
    63241 cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc
    63301 taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc
    63361 ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga
    63421 actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga
    63481 aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca
    63541 agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa
    63601 gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga
    63661 agagtacgac agagataact tgattgatat ctcttattac ggaccaggta agaaatacat
    63721 tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac
    63781 tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt
    63841 gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg
    63901 agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata
    63961 aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac
    64021 acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa
    64081 atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg
    64141 gactgggatt atatgtattt caaggggaag atattccaac accacctaga atcgatacaa
    64201 agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata
    64261 tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg
    64321 ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa
    64381 tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga
    64441 ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga
    64501 ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat
    64561 tgaaattcgt tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc
    64621 gctgataggc gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt
    64681 ctactttcgt tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac
    64741 aactgtgagt gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca
    64801 tattcctttc aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa
    64861 atgcatcaca aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt
    64921 tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc
    64981 agcactctgc agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa
    65041 taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg
    65101 agggtaaaaa tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac
    65161 gacaaaagac ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat
    65221 gaaagaggct tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa
    65281 gagaccatat cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc
    65341 atttacaaag agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct
    65401 attgacgaaa aacgcaatac ccctattgac gaaaaagtca aagataataa tacatctatt
    65461 aataatacat ttaataatac aaaagaatat ataagagagt taccgccttc gaaaaaatcg
    65521 aaggctaagc ccgtccgtca taaatacgga gagtataaaa atgttctttt gtcagatgag
    65581 cagatggaga aactcaaaac agaattccct aatgattacc aagagcgaat cgaacgactg
    65641 tcagagtatt gtgaatcatc tggtaagact tataaaaact atttggcaac tattcgaagt
    65701 tgggcaagga aagaaaaaag tgaacctaag aacgcaagca gtggatacaa gcgcacagga
    65761 agacgagaga agcttcctga atgggcaatc gaccaagaag cctatctcaa gaaaaaagcg
    65821 ctagaacgag ctaatagaca atcaaaagca ccattctaag aggtggaaaa atgaagatcg
    65881 attatctaga actaattaat gaaatagcga attataaaaa gggcgaggaa ttagacgtcc
    65941 tgagagacgt atatgatcaa ctcgaagaag ctggaattga aggaattaag aatgatcgtt
    66001 cgagttggag taagctcaga tactatttcg cactttatat cgatacaaca caattaagaa
    66061 atttagcata taccaaatta ctatttgttg attgtgttaa aggcttgcaa aaacatctta
    66121 atgaacttga gcaggtgtaa tcagatggac ctaaagacat ttacagcaca gattgaacta
    66181 atgcatcaag aagctttaag acaaagtgta tcgtacgaag acaagtggct caacacgttc
    66241 catggcggac gtgagagcgc acttgatcaa gtactcaaat tactgaaagg agaatgtcgg
    66301 gatggataag aaagcggcaa tgcagcgaat tatcgaattg acttattcag aagattggca
    66361 aaatgacaaa gaagctgctt cagaagtgat gaggcttgga agagcgatgt gggcagacaa
    66421 gagcaacaag ccaagaccac gaaaaatcgc aatttggcac ggtgacaaac ttctagtgat
    66481 agggacagct gaacagttag caagtctcac aggcttgcac gagaaaatcg tgaggaaaag
    66541 agcaaggtgt ggctacacag acgctaagaa gagaacgttt agatacgtgg aggaatcatc
    66601 atgacaacag aagaagtgat tcaaatgcgt attcgaagcc ttcagcgtga gattgacgat
    66661 ctggaacgaa caaaggcagt gatggtcaat gaaacggcga gaaaggcaat cgatttgcac
    66721 atagagaatt taagaaggga aatccatcga ttggaggaat gagcgtggat aagaaagcag
    66781 caatgaaaag aattgctgaa ttaaccaagt cagaatcttg gcaagaagac aaagaaatag
    66841 ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga aaagcccaaa cggagaacgc
    66901 cgagaaaaat tgcaatctgg catgatgacc gaattttagt aacaggtact gctgaacagt
    66961 tatctgaaat tactggatta agcaaaaaca ttatctggga tagagctagg agcttatgga
    67021 ttgattcaaa aggacgacag tttaggtatg tggaggagag ataatggatc tcattacaca
    67081 atacagtgac atcatcctca agaaaatcat gatgaagatt cagaaagata aaaaatcaaa
    67141 agaacgagct gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag
    67201 caggcattgg aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt
    67261 cgatcagatg cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg
    67321 tttcaaattt gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa
    67381 tgataaaaaa actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac
    67441 tatctgtgga atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt
    67501 gggtagttga tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg
    67561 ttgagtttga aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg
    67621 aatgataccg aagtttagag cgtgggataa acgaaagaac gtaatgagag atgtagccgt
    67681 cttgcatttt actaaaaacg gcaaaacaaa ctttattgaa tattggataa atcctaccga
    67741 attgaaatca tatcacgtgc gaaacatcga cctcatgcaa tccacaggaa tgaaagataa
    67801 gaatggtgtg gatatatttg aaggggatat agtattagtc agcgtgcgaa atggcttcga
    67861 ttacttagat aataaagtct gtattgtcaa aaattcaata gattattccg gattagtttg
    67921 tgccactgtt gatgaagact tagagtatca aatttttaac acagagctgt ttgaagaata
    67981 cacgtacgaa gtcaccggaa atatatacga gaatagcgag ttattggagg aacagcgatg
    68041 aataaacagg aagcaatcaa aaagttagaa agtattaaag cgataggaaa tgatgcaata
    68101 gctgcttgct ataacgagag tataaattca ggtattacgt taatgaaaaa aatagacgaa
    68161 ccgcagaaac cagttattcc acaactcgtg gccggttggc ttgagaaatc tacggaccct
    68221 tttacaaaag ctgaaaaaat agcgtattta atcaaatcta aagatggtga ttcatattat
    68281 ttctgtgatt ggtttgtacg agatggcata gtgacgcaag agcaaggaga agaattactc
    68341 gcttgggcaa caagacaatc atatgaaaca ctattgagcc tatacaacgg ctacgaggtt
    68401 gagaaagagc cgttatgggc aataaagaat gccgatggaa actatcttac taaatgtgct
    68461 ttatggggaa aagatggagt aaattatagt tttgaatgca atccatctca tcgattgctt
    68521 ttcactgata aagcaacagc ggatgctgca gcattgttgg tgaatggaac agtggaagag
    68581 gtggtagaaa gatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt
    68641 taatgctaga tatgccgaca aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg
    68701 gtgacatggt ccgctgtgaa gcagaagagt tcgtttatcc gttcagagga tacgttaaaa
    68761 agatactgtc aaactcagca atcattcgta ttgaaaatac aatgaagtgt gatcgttcga
    68821 ctgcaaaaca caagcattat ttagctgtag cgagattagt tgacatggaa gtaatcaaga
    68881 gcaaataaaa aaagccggat cgctccgact gattcaataa atccaacaca tttattatat
    68941 cacataaagg agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca
    69001 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc
    69061 tctttgatag atattaagtc gccgattata acggatatgc cgaaggcacc gaagcatggc
    69121 aataaggcag aagacgcgat tattcagatg atggatatag aaacggagag agatgcgatt
    69181 ctagcggctt tgatggctct tagtctgatt agtcgtcaga tactctacta cagcttctgt
    69241 gtgccagata gcttctcaaa ctacagaatt agccgtgaag tgggttattc agaaagaagt
    69301 atacaacgga tgaagtcgga agctctaata gagtttgcag aagcatataa acacggaaaa
    69361 ataattgctt ataaataatt tggcggtttt ttggcggaat gatggcggtt tttagacatt
    69421 taccagtgtt attatggtag tgtcgaaaga ttaggaaaca ggatcgacaa aataaacgaa
    69481 gggaggcaat ctccctcatc gttttaaact tcttcttaga tagacagcaa ctaattatga
    69541 agaaaaggat gtgaattcaa ctcctcctaa attcttcatc ggttgctgtt tattttgatt
    69601 agacagcagc actcacaaaa ctaaacatgt tcttgtcgtc tttcaactgc tgctgtctat
    69661 taatttatgt attggaggga agaaaaatga aattatcagt agaaggcact ccagaagaaa
    69721 tacaagaatt gctccaagct attggtagta gcaaggagca aaaggtaagt gtttctaatt
    69781 cagatattga cggtatttgc aattgtttaa acagagaaaa aatatctaat ccttactaag
    69841 agaaaatatg ttcgttaatg aatttccaat tttcctctgt agctctacct tgataatttt
    69901 tttgcagttc acagatgaat aaggaatcgt tattatcaag tactgtattt aatttttcac
    69961 acatttgatt tggattcaaa ttagttttaa ctaaccaaaa agatttttgt agctttatat
    70021 atgccccaaa ttcttttatt atttcaaata ctttatcata cttttgccct gggttattaa
    70081 gatcataact aatcatgtag ggtttataca tgttgtcttg cacatccttg ttttatattt
    70141 cagcggacca ctcgctgata aataaaatta tacgcttagt atttattttc acaatattaa
    70201 tttatcgtaa taacttttga gtaaatagaa caaaaaacct gcactagttt ccgctagtac
    70261 aggcagtgac tatatcggtt gataatctag catatattaa aaataattgc aagaaaagaa
    70321 atttatagtg gtttatacca aattatcaaa gcaatgttat tttgttgctg tctattgttt
    70381 tttaattatt cacacgataa ctaaaggtgg gtgaagagaa atgattccat taataatttc
    70441 aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatgggc aaaataaaaa
    70501 atagtagcag ccaagaataa ttttatagtg tcactgtggc ggaaagggta gacgctaagc
    70561 atgtgtgcta ggtcaatgct tcggcaacca tgcaaggttc gattccttgc cagcgacatt
    70621 aaatgcctat gacggttacg actaccgaaa aaagatcgtt aagaagctat acggtgctac
    70681 gtacggcaat gtagtaagtg tgctatctgt acaccaccaa gcttcggtca ctgtggcgga
    70741 agtagaagac gcagcggtaa atggcgagta gcctcgtgag agcctggtaa gttctcgtga
    70801 gtggtgcaat cccactccag cgactttaag caaccgaggc atcggcggtt taaaaatata
    70861 ggggtgcgca atttcgtacg cgttttgtgc atcgtgcaag ttactattac atattagatc
    70921 actctttgag tggtcttttt atttttgcac aaaggaggaa acaacaatgt atagaccaca
    70981 atacttagaa cagaagtatg aagtaatcac tgttcataaa ggtaatggcg aaaaagtata
    71041 tgagtataga agaccaataa agagcgatac atataaacga aaggaaaaca atgaagttat
    71101 tccattgtat ggcaaaagaa tagctaagca ttaaataaga ttgcgaaagg agacggaaca
    71161 tgaccgagga attctataga tggctattac agttgacaag agaagatcgt ttggttaagt
    71221 tctatcagtc tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg
    71281 aatgccaaga gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa
    71341 aggaagtcaa ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg
    71401 ttgaacatca taatgaagtt catggcagat atcttacagc attagataaa caagagaaga
    71461 agatagaaag ctttgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat
    71521 gataatggca gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga
    71581 ttaattaaac gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac
    71641 tgttgtagta aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc
    71701 gaaagaataa aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc
    71761 gctgaagctt tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt
    71821 gggggtgatg aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa
    71881 aggagggcta aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc
    71941 cgctctttaa taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat
    72001 gttgatcgtt atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag
    72061 aacggcatta gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc
    72121 aacaaacaaa tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa
    72181 gcaaatgaat caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga
    72241 aggcactatc aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt
    72301 ctttactaga gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc
    72361 agcacaatac tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta
    72421 tggtgttcga tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg
    72481 acgtggtaat ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa
    72541 acacggtatt aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac
    72601 tagttttgat gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt
    72661 taaagctacg aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac
    72721 ggcaaatgct agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca
    72781 cgaaaatgaa gattattcaa tgataaatgt ggctacttct ggtggtggta aaattcgaga
    72841 ttatagagaa ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat
    72901 tatagaagaa tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga
    72961 attttctagt ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc
    73021 cgacatgtgg gaaaaagctt gtccaactat taattacaat gcagatttaa aacggaaaat
    73081 gtttcaagaa tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa
    73141 acgaatgaac agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct
    73201 gcatacgaaa gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt
    73261 tgctgataga cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta
    73321 ttttacacaa catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga
    73381 ggttatagat atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga
    73441 agctgattat attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat
    73501 cgctatggat atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac
    73561 tgtggaaatt gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga
    73621 cctttttatt aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat
    73681 gaatgtatat gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga
    73741 aactagaaaa acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat
    73801 ttatgattct gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt
    73861 tactcctcta gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg
    73921 taggatactt cacaaaaaaa gcaacggttc ctttagaaga atacttttgt aaattgcaag
    73981 ttgattttgt gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga
    74041 tgagtaaagc ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact
    74101 ataggctgaa tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga
    74161 tcaggagatt aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg
    74221 aaatatttat tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt
    74281 ctcaagtgca aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct
    74341 atataaaata cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg
    74401 ggaaactcat ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac
    74461 tgaaagggaa tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga
    74521 tgacatcaca atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac
    74581 aaaatgagta cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa
    74641 aaaacttaat agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc
    74701 tactaaaggg agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa
    74761 tcataccgat tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag
    74821 aatacttgtc agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca
    74881 aagatttggt tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag
    74941 gaagagaacg cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa
    75001 ctaaaaacaa tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt
    75061 ctagcagtaa agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct
    75121 agttggttct ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt
    75181 gatcaagaag aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt
    75241 gctattggga acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta
    75301 gccgctagtg ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat
    75361 gcacaattga tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt
    75421 acgattgctg accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga
    75481 ttcaatggca cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca
    75541 gaaacagctt tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt
    75601 actgaagcta aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga
    75661 gcagaaaaaa ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta
    75721 aaataatgac agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag
    75781 cttttaaaga tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa
    75841 ttcaggataa aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga
    75901 accgtggtca gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa
    75961 acgaaggttt tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg
    76021 aagatttaga acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg
    76081 taacagaatg gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag
    76141 ctgttaaaaa agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat
    76201 caggttatat tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt
    76261 atgtccgtac tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg
    76321 gtactggtaa agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag
    76381 aatatgctga aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt
    76441 tgatggcgcg actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg
    76501 tgaatccttc tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc
    76561 cagctaagac tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt
    76621 cagatttgca gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt
    76681 acttcatggg tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg
    76741 aagacgaacg catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata
    76801 gtttcattgt attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa
    76861 ccacaacaac tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg
    76921 caatcaatcg gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa
    76981 tcaaagatat cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga
    77041 aggaatatgg tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc
    77101 cttcgattaa accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg
    77161 accgatattt ctatatcgat tcggatgtca ttttcaataa aagaattaat ttgcgcaaat
    77221 tgccttctaa agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt
    77281 atattagaag ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg
    77341 ttacagtaga atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta
    77401 accgtcctaa agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct
    77461 accttagagg gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt
    77521 ggaacatgat gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg
    77581 ctactgatcc aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa
    77641 caaacgatga agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag
    77701 atttatcatt tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg
    77761 tggttagatg acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat
    77821 ttacgttacc tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt
    77881 ttatcttcaa agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg
    77941 attgctaatc gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa
    78001 tttcgctagt gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg
    78061 tgatgtgaat ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga
    78121 ttttgacgca aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag
    78181 aaatcacttc tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg
    78241 atgcgatggg atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga
    78301 aatttaataa agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg
    78361 ccgattacga taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt
    78421 gataattcta aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta
    78481 gaagagcatt ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat
    78541 gattttaatt acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat
    78601 agcgtgggtc aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga
    78661 gactcactag atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat
    78721 cccaatcatt taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt
    78781 agacgattat tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg
    78841 acatgatgaa cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa
    78901 cgaagcattg aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc
    78961 tgtttctgaa gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa
    79021 gactcaatac attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa
    79081 atatcctgat ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag
    79141 aggattaggt cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt
    79201 aaataaatag ggggaacaac aatggctaaa acaaaaactg tagtaacaac gttcgataac
    79261 gtaagtatca aacgaattgc ttttaatttt aagaacgcag gaaatgcaat cgcaacgaat
    79321 tgtaacggac aattagatgg cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg
    79381 acagaagtaa aatcaaaatc taaaccaatc aatatgacgg taacaattac tgcacatgta
    79441 ccgatggaag tttatcgacg tttcaatggg ttgaaacaag atggacgtat taaaccaggc
    79501 atttactctt acggtcctga ttccgtaggc gaagatttct cacttgctgc agagatcgtg
    79561 gatgacttcg aagaaaatag aaagttagtt ggtatgttag catgcacttc gaatacagga
    79621 ttaacattct ctattgaaaa tggtgcggat gaagtagctg cgttagaact agaaacaaaa
    79681 gttatgcaag atgaatttgg taaattttat catgaagcaa ttgttgcaga acttgaagaa
    79741 gacttaacag atcaatggat gacgaatcta tctgctgatg tgattaaaaa aacttcagtt
    79801 gtgacaacta cggccactca atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga
    79861 ttactcaaaa attgaactaa acgatggaac aattttgaat ttagaaccta aactgaatat
    79921 caagaaatta ttgatgatca atagagattt taacacagac gagtttgcaa aaatgactgt
    79981 gggaaaagga tccatggata tttctgttat tcaaggtgca aaggctgtgt atattgctta
    80041 ccgccaagcg aacatgactg attatatttc attcgatgaa tttatcgata aatgggattt
    80101 tgatatggct actgccagct atatttatca attgatgatg ttcaaacaag cacgcgatgc
    80161 ttatcaaaaa gaatttgaaa aagcaaataa ggaaaaaaag cttcaaaagt aaaaattcca
    80221 aagctcttag ttgaaacgtg ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg
    80281 ccttcagatt tggttttaag cgatatctgt ttagatgaca ttttgcaaat ggcttacaac
    80341 aagagtgctt atgaaggatg gaaaaactat gcaataaacc aatcccagaa aaactaaaga
    80401 aaggaggtaa aaaatggcta aaaagagaac agaagcagaa gtaactttca tagctaacga
    80461 tgacggattg aaatctacgt taaaagaaat cagtgctgaa ttaactaaaa atagagcaga
    80521 attaaaacta gaacaagctc aattacaaca gactggttct gaatcagaca agttaggaag
    80581 taaattatct tctttagaaa agcagtatga attacaaagt caaaaagttg aagtaactag
    80641 ccaacgttta gccaatgcga aaaaatatta tggagaaaat tccaccgaag ttaagaaact
    80701 tgaaagagaa ctgattaatc aacaaacagc gcaacaacgt ttgtcaaacg aaattgataa
    80761 aacgagtaat gcactagctc aagcaaaagg cgaaatacag acgtacgagt ctacaatgca
    80821 acagttggat agcgaacaaa aaaatgttca agctagtgct tctctgattg aatccgaata
    80881 caaaaaatgg caagcaactg ctggtcaatc agcttctgaa gccgagaaat tagcgaaagc
    80941 ccaagaatat gtttctcaac aatctgaaaa tgcggagaaa acgatagata tcctaagacg
    81001 acagttagaa gctacacagt ctgaatttgg cgctacatcc acagaagcaa tgcagatgga
    81061 agcgaagctt aatgatgctg aacgtgaatt tgaagagtta ggacaagctg ctaaaaatgt
    81121 agatacaact aacttggacg atatcggaag caaaatagat atgaataatt taatggaagc
    81181 ttctgacgtt ttaagcgaca ttggcgataa gcttacagaa ttagggaaac aagcagtgga
    81241 ctctgctaac agtgtaggta gttcccagag taagatacaa gctaattttg gtttgtctaa
    81301 acaagaggct gaagaattaa cgaatgtagc cagagacatt tattataaag gttttggaga
    81361 atcgttagat cagtccacag atgcattgat tttggtaaag cgtaatttag gagatttaaa
    81421 taatcaagat ttacaaaata tcacggaaca agctatggtc ctagaaaaca ccatgggcgc
    81481 tgatatggat gaaacgttac gtggtgtaaa tggcttaatg gtcaatttcg gcttgagtgc
    81541 tcaagatgca atggatttaa tggtttcggg tactcaaaac ggtttagata aaacgcacga
    81601 attaggcgac aatatggcag aatatagcca attatggagt caaatgggat attcagctga
    81661 tgaaacgttc ggaatgcttc aaaatggttt agatgcgggt gcttataacc ttgataaagt
    81721 caatgactta gttaaggaaa tgggaatatc gttaacagat ggtcgatttg agcaaaacat
    81781 ggatatgttt agtgaaagta ctagaaaagc ttttgaagag tggaaaaatg gcggaggaac
    81841 acaaaaagac gttattaatt ccatgattca agattttagc aatatggatg gtcaatacga
    81901 ccaattaaat aaagcttcga caatttggtc tgcgcttggc gaagataatg cgatgaaagt
    81961 tgtccaatct ttaactgatg ttaaccatac atttgatgat gttagtggat ctgcacaaaa
    82021 aatgaatgaa gattctacta ctccgttgca agagttgaac gggaaaatag ctgaattaaa
    82081 ggattcatta gctcctatag gcaacacaat catagatgca ctcgaaccag taattgattt
    82141 tctaggaaag atggctgatg cgtttaataa tcttccacaa ccagtacagg atttcattgt
    82201 tgctataggt ggtcttacag cagcatttgg catactgtta ccggcaatac tagccgtgtc
    82261 atatttattt ggtccgatga tgcttataat tggaggaatt atagctgtta tagcaggcgt
    82321 tattgtggca attaagaact ggggtgcaat tactgactgg tttagtggtt tatggaaaaa
    82381 atttactgat tggttgggtg atacttggga aagtataaaa gacggagcct catcagtttg
    82441 ggatggggtt aaagaaacct ggtctggatt tgtagattgg gttcaagata tttggcaagg
    82501 agtttctgat tggtttggag agctatggag cggattagtt gaaggagctt ccaacatctg
    82561 gcaaggagtc caagaaactt ggcaaacatt cgttgattgg gtttcaaata tttggaacgg
    82621 agtcaaagaa gtatggtcga ttatttgggc agacattgta ggaattgttc aaataccatg
    82681 gacattaata acgtcattga ttcaagctgg tattaatatt atcgtgggta tttttgatgt
    82741 agctggacag tgattgggcg cagcttggca agctgtttgg acacctattt ctgatttcct
    82801 taaaaatact tgggatacta tgacacaatg gataagtatc gcttggaatg ggattgtaac
    82861 tacattccat actatatttg atccagtagt ggcatggtgg aatggtatat ggacagctat
    82921 tagtactacg gcttcaaata tttggaattc aattagtgca acagcttcta gtatttggaa
    82981 cagtatcaag aatacaatca ctagcttggt acaagcagct gctacagtaa ttcaaaatat
    83041 ttggtcaact gtatctagtt ggttaggtgg aatttggaat tcaatcagct ctacagcatc
    83101 aaatatctgg aatagtgtga ctagtagtat aagcaatgct ataaacgcag ctaaaagtgc
    83161 cattcaaagt gtttggaata gtatatcttc gtggattagc ggaatttgga acggtatcaa
    83221 aaatactgct ttgaatcttt ggaatggaat tacaagcact attagctcta aagtaaacga
    83281 tggaaaaaat gcaatttcaa gcggttggtc caatctaaca ggtattgttt ccgacatatt
    83341 caataatgtt aaaagtacaa ttgctaacat atgggaaggc atcaaaaaga ctgttagcgc
    83401 tccaattgat tggattagag acaaaatcag tggcattttt gataatttga atatttctat
    83461 accacatatt ccgttaccgg aatttatcat ggaaggcagc tttaacccgc taaaaggtca
    83521 aatcccccat ttgcgtgtaa aatggcatgc taaaggaggt atctttacta aaccaacttt
    83581 actaggtgga atgaacggtg tcggtgaagc aggacctgaa gcagttttac ctttgaaaag
    83641 atctgttttg caagaaattg gtgatcgtat cttgagtagc acatcagttt catctagggc
    83701 acaaacgatt caacctgtga ataactacga attcaatttc acaattgatg gtaacgcaga
    83761 tgaggttact atgaagcaaa caactcaaca aatcattgat agcattacaa aaattcaaaa
    83821 tgataatgct tcggcatggc gttaaacagg agagtatttc tcctgttttt ttagtattaa
    83881 aaaggatgtg aaaaaatgac tgattgtata cattctataa tcgatggatt tcctgattat
    83941 ttgcataaat tggctttagc tgaaagacca accatacctt ctccaaaaag acagagagtt
    84001 gaaacttctg ttttaggtag gttaggtggc ttagtacaag attactcgtt tgaagatatg
    84061 tcgtttacat tgcactataa ctatttagag gatgtggaag accatcaagc gttcaagcaa
    84121 tcgttttata tcatgcgtca ttggttaaac tatgcaaaga aattagaatt ctctgacgat
    84181 cccaacgtct attacgttat ccagactatc gatattgggg atgcagaaaa cgatattgtt
    84241 gaatggggag agttcgatgt aaatattact gcgaaaccat tcgcaagagt tcaagaagat
    84301 gtacctataa ccgtagataa accacagtca tttaacttgc tgaataatag tttagaagaa
    84361 agttttccaa agattatcat cactccttca gctacttcat gccagttcat cttaaatgat
    84421 tatgtgttta gtttcgaagg cttagtagca ggaactgatg tagtcattga tagtgatttg
    84481 atgctttgct acgaagagca atcggacgga gatattttag atcggtccaa caaaatgaag
    84541 accatgcaat atccgacatt gcaagtggat atcaatcatt ttaattgcac tggtttgagc
    84601 aaaatacaaa tttatcgtaa cgggttaagg taggtgaaat agatgatcga taatttaata
    84661 actatttacg ataaaaatga cgcgaataat ttagctgaac atttatatga tacgcaaggt
    84721 ttaggcgctt tgtcagactg gttaacagct actgttagca ataaactaaa cggagccgag
    84781 atatttcagg gtacttattc aataaacgga actaatgcag atttgattgt agacggacgt
    84841 attattcagt gttatgtaga tgaaaatcga gcaaaacagc gtctacggat ttattatgca
    84901 aaaacttctg taataggcaa tacgatagaa gtaaaagctg aacctatttt caatgatata
    84961 agaaaatcgg tgttgaataa atatgacagc ggaacagaaa agatcactgc tactcaggca
    85021 tggcaaaacg caaaagtttt agcgaaacca gctatccctt cgcagttttc tttctcgtca
    85081 ttagtagata cgcttgctaa tgtgaagata gaaaaggcga attttttaga attctttggt
    85141 ggaaaagagg gatctattct agatcgattt catggtgagt ttctaaaaga taataacaca
    85201 ttacgtcatg aaaaaagtct aggtacggat cataaaatca aagcgattta tactaaaaac
    85261 ttaactggtc ttgacttaga gattgatgct caaagtgttt tagttggagt ttatccattc
    85321 attagcagct cttcagaagg agaagacgag atcactctac cagaagaagt tattttcacg
    85381 gattacgtgg atgattatcc tgctggatat gtttcttttg ttgattttaa agacaaagcg
    85441 actgatgtag ccacattaag ggaagctgct aaagactggt tgaaaacaaa catagataaa
    85501 caaaaaccac aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca aagaggctat
    85561 gaaaaatttg ttgatctaga aaaagtttcg atgggtgacg gagtagatgt gtatcatcca
    85621 cagttaaaag tgaatatgtc agcgagaatt gtggaatata cgtttaatgt tctaaccaat
    85681 tcatacgata aattagttgt aggaaacgtc aaaacaaact tcttagaaaa cacagagaat
    85741 aatgtcagca atttgattaa tgatgccatt gatcaattga aaaacggtgg cgaaatcagt
    85801 gatttactca atgatattgt agatcatcaa actgatatga ttactggtca aaatggtggt
    85861 tatgttttat tagatcctaa agaagcgcct agtcgtattt tgattatgga cacaccagat
    85921 aagaataccg caaggaatgt tttacaaatc aacaatgctg gtattggttt ttctaaaact
    85981 ggcattaatg gaacatatga aacggcatgg acgttagatg gcggattcaa tgcctcgttt
    86041 attacggctg gtgaaatagt aggaattact attagaggta ctacattaat tagtgatggt
    86101 gctgattata gaacaagtat tgctaatggc aaaatgactt ggtactcaaa aaaagttaac
    86161 aaagatatta tggagctaga agcacgtgat tatgtaagtg ctgatgccgg tattgtatca
    86221 tacaccatga aaactggtgg tggtttcatg attagaaatc cacagggtaa cttggttttt
    86281 agtacgtggg ataatggtaa taacagaccg tttctatctt ttggtgcgcc caatttcagg
    86341 tatagcaatg ctagttatgt aacttctggc gacggtagtt ctttaagcat taatggtagt
    86401 gcgggtaact catgggaatt taaggtagct ggtaggacta tgaaatttac tagtgatggt
    86461 atgctaacgt taccaggttg tttttttggt tcatgggaag atgggaaact tgctaggttt
    86521 gaacaatcaa cggtacaagt atataaagat tttactgtta gaggtactaa aaactcaact
    86581 gtaccaacag aacattatgg acaacgacta ttgaacgctt atgaaactcc agaatattat
    86641 ttcgctgatt atggggaagc cgttacaggt gacaatggta aagttcgtgt tgatattgac
    86701 cccatgtttg ctgaaacagt aaatctaagt cggtatatga cacatgtgac acctacagaa
    86761 ctagttttgt gtgctgttac tcatgaagat attgaccatt tcatcattga aactagtaag
    86821 ccaaacgtat tagttagatg gaatttagtg gcacaccgtc tagggtatga agatattaga
    86881 ttaaaagagg atacagcata tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa
    86941 gacaaggagg tatataaatg gctagcagtt tatataattt ggctttagat ttcagcaaag
    87001 aattaaacta caccaaagct attatggctc gtcaaggtga taaagggatt acggtgacgg
    87061 ttaaaccgtt tctaaatggc ttgcagatgg atacgagtgg cggaacattt actttaaaag
    87121 gaacaacacc atctaaccgt tacgtagata atgttgcaac tagtgtaact agtgaagaag
    87181 tcacgttttc tcttgatggc acatttatga gtgaagcagg atattataaa cactgctacg
    87241 tagaatatag aaaagacaat caaattttaa caacgcaaga tatcattttt ttctcactag
    87301 gagtgtctga catttcgcaa ggccaagccg atgaatatgt ttcgcaatta gaagagttga
    87361 ttcgaaagta caacgaaact tttgatgctt ttatggctga aatcaaaggt agagtggata
    87421 gcttaaatca acagattact gatttaactg gtcaagctaa aacgctacaa gacaagttag
    87481 atgctctgaa agaagaaatt tctaagttag gtaacttaca agtgatgtac agtaacagca
    87541 tcgacttcgg ggactatgat tattcggaga acccgaattt aatgccatat attacagaac
    87601 cttgggttgg acctttgtta ggtaatggac atacagttaa agatagtgtt aaaagggtta
    87661 taactcatac taaaactaga acagctaatt ccggcgacat attatcgctt ggcttaggta
    87721 ttccgtgtac agcggaagct aataataggt atcttataac cactttacga ccatctacga
    87781 catacacatt aagcgttact atgagtgtcg gttcagattg gactggagag accaatacta
    87841 taggggttag gcttagatat ttaaatgaac aaggtggtat agaactgccc attaatgcat
    87901 taataccagc aaatgtagaa cgcgataaaa tggtcactca tacatttact ggtataacaa
    87961 aagataatgt aactagcata acaaactgtt atgttgaaat attctcactt aatagtgaat
    88021 ataaaggaac ggttagtgtc agctatgacg ttaagttaaa agcacactac cctaacttac
    88081 tagatggccc ttactggtta ggtaaagttc ctttaggcga gaatattgct gaccctacag
    88141 ttgtctttcc acacaaaact agtgaatata tggtttatgg tagacgcaat acagaaaact
    88201 atatcgctga ccaaacatat accatatcaa tgaaagcaac taaactaaca gttcaatcgt
    88261 ttgccgtgta tattgctgct ggccgcgtta aagtaggaga catgaagcct actgaggggt
    88321 tagctaatac atgggagtta acttttactg taactaagca acatatagat agtggggtaa
    88381 ctaattatct ggaaatatac caatacccgt cagctacaaa aggagctgtt caaattgagt
    88441 ggctcaaact agaaaaaggc aatactcgaa ccccgaatat tagtgagtat aaatatcgtg
    88501 gtactggtat gcgtgattca aacaatccaa aagattatgt ttgggatcta gcaccagaat
    88561 atgtcgaaga taatttggcc acagatatta aaatttctga aattactggt aaagcaaaca
    88621 attataccga tgggaaagta tcggagatta attcgcagtt gactgcttca attaatgaag
    88681 tagacaccac agctaaggat gctcaaacaa aagcgaatgc taatgcgact gctatagatg
    88741 aattagacaa taagatcgat gaacgcatta atgatacagc tactaccaca ttaacagtta
    88801 caaacgggaa taccggatca gcaaagcttt atcgtgaagg aaaaacagtt tctatatatt
    88861 ttgtggcttt aaacggaaaa agcagtggtg gaaatgattc aacgatacta acaattccag
    88921 aaggctatcg gccaccaatt agttttgagc aactggttgg ctcgatagac cgttctactt
    88981 tgaacagtgc tcagttatct attggtgcag atggagccat taaatggcga agaaactcaa
    89041 gttatggatc ggattatacc tttgcaatta cttacacgat ttagaaaagc gtgaatcgat
    89101 atgaaggcag catatagacc aattgaacct tacggattcg agcaaatcat tgtgaatgat
    89161 gaagaacatt taccggaaga atgcacagaa gtcgaaccac cgattccaaa ttggaaaccg
    89221 aaattcaatt actgggaggg aaataaatga aaaacatttg gaaatatgga cgtactggcg
    89281 gagagtacgc aggaaaagta ttggacgaca tgcttgtatc cgttccttac acagatcagc
    89341 caccgcttga aggaattcgt gctgatggcg aaccgttaac aatcgctgat caaatgtttg
    89401 atcctaaatt gaatcaatgg attattttag cgaacgcact agatcacaac gatttaaaca
    89461 atctcaaagc aatgtatgag tcgttagaaa atgagaacgg cgatttaaaa cagatcaatg
    89521 ccaaactcat gctaagcgat gtagcaatta aacaggaaaa tactgcattg aaagaaaaag
    89581 cggatagttt agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa aatagcaaag
    89641 acatttcaga aattaaagag caactaaatc cagcttcaaa gggaggtgag tagtatgttt
    89701 agttttagcg atgtgaaaat gatgtatgat tggggctgtt tcactaacga acaagtaatg
    89761 gttttcgttc cgttgtgcat tactgaagaa aaagcagata aaatcattag caaagaagag
    89821 agcgcatctt aattgatgtg ctttttattt tgattcaagg agttgtcaca tgattaattt
    89881 aggggaatgg ggagcgatag caggatcaat aaccgctatc gtttctttga ttttattagt
    89941 aataaaacca attactgcat ctttctcgaa gattactgag actctttcaa aagtaagtca
    90001 caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca
    90061 tgaagaacta aagaaacacg atgaaagatt agatacacat gcagaaaaat tggtagaaca
    90121 cacgcaacaa attaaaactt tatttaggga gagaagaaaa tgaataataa aacgttcgaa
    90181 gtactaaaat ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt
    90241 ggtaaagcgc tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc
    90301 gcgtttttag ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa
    90361 agcgaagaag gaggaaacaa atgaaaaaga aaattactat tactgcgatg agcctgttaa
    90421 cggctctttt tttattaccc attaatacgt ttgcttatac tattaatgac gagtataatt
    90481 tagcgccgaa tcaaggagac tccagattag caattcctaa caaaattatt ttgcacgaaa
    90541 ctggaataga tgcaccagca agaaacgtag ccgccaacat gaaaaataat tataacggaa
    90601 gcaatcctta tactacagat gttattggtg acggtgggat tgtttaccgt gtgggtgagc
    90661 aaggatatgt ttcgtgggga gctggtaacg ctaatcctta tgcgcctgta cagattgaat
    90721 tacagcgcac atatgataaa gcattgtttg aaaaaaacta tcgagcttat attgaatata
    90781 caagagatag tgcaaaaaaa tatggaattc cattgactct tgatcaagga acttctttat
    90841 ttacaaaagg aatcatttct catttgtggg tgacaaatta tgtttggggg aaccacacag
    90901 atccatatgg ttacttatcg caaatgggag ttagcaaaga aaagcttgct tatgatttag
    90961 ctcatggatt taccgatgaa aatccaacaa cttctgaaaa caagcctgtc attgatccaa
    91021 cacgagctgg tgcagctaat cctacgctga cagatggaac aaatcacgcc cacattgatc
    91081 agtttgggga aatcgaaaac gcaaacttgc atgtggctgg atggcacatt gctaactata
    91141 aatacgagta tattttcatt atggactaca atactgggaa agaattagct cgagtaagag
    91201 ctgatggaat ttatagacca gatgtaaacc aagcttataa tacttctgga aatgttggtt
    91261 atcatgtatc tttcaatatg cgtaattttc ctagtaagaa agtctatgta atgatgcggg
    91321 caacgaatga tccagaggga aacactaaag gcggtgcgca agatttccat gacaaacgtt
    91381 ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag tacataacta
    91441 tattgacaac tataaaaatt attcgataaa atagtgatgt tatcgcatat cttcactatc
    91501 acccataaat agtcacactc caagctatgc gataacaggt ttgttgccac acattctact
    91561 ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag ttggtcagag
    91621 cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat taacgtagcc
    91681 atttgaatcg ttctgtgtta gaattttttt gaagagtatt atacaagcta aagcttttct
    91741 tcattgccac tcaaatgagt ggctttttta tgtatccttt tatggattaa tgaaaggatg
    91801 tttcacatag ttatacttct gtatatttga aaagttttac tttgattttt aaatagaaag
    91861 acatttgggt taaattgtga gataataata aagaagagtt taaagcgcac cccaaaccac
    91921 ttccccataa gtgtgttacg ctttaaactc ttttatattt gaagccatta aaaagc
//
LOCUS       DAFCLG010000002        76211 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFCLG010000002 DAFCLG010000000
VERSION     DAFCLG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.002"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..411
                     /locus_tag="LND56_000118"
     CDS             <1..411
                     /locus_tag="LND56_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6742072.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="LND56_000119"
     CDS             509..697
                     /locus_tag="LND56_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6742073.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="LND56_000120"
     CDS             complement(755..1108)
                     /locus_tag="LND56_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742074.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="LND56_000121"
     CDS             1639..2061
                     /locus_tag="LND56_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6742075.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="LND56_000122"
     CDS             2058..2288
                     /locus_tag="LND56_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742076.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="LND56_000123"
     CDS             2786..2986
                     /locus_tag="LND56_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6742077.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="LND56_000124"
     CDS             3013..4206
                     /locus_tag="LND56_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6742078.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="LND56_000125"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="LND56_000125"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK6742079.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="LND56_000126"
     CDS             complement(5898..6821)
                     /locus_tag="LND56_000126"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK6742080.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="LND56_000127"
     CDS             7017..7622
                     /locus_tag="LND56_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6742081.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="LND56_000128"
     CDS             7930..8946
                     /locus_tag="LND56_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6742082.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="LND56_000129"
     CDS             9087..10388
                     /locus_tag="LND56_000129"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK6742083.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="LND56_000130"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="LND56_000130"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK6742084.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="LND56_000131"
     CDS             11117..11515
                     /locus_tag="LND56_000131"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK6742085.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="LND56_000132"
     CDS             11581..12663
                     /locus_tag="LND56_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6742086.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="LND56_000133"
     CDS             12875..14437
                     /locus_tag="LND56_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742087.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="LND56_000134"
     CDS             14430..15563
                     /locus_tag="LND56_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742088.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="LND56_000135"
     CDS             15563..16519
                     /locus_tag="LND56_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742089.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="LND56_000136"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="LND56_000136"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK6742090.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="LND56_000137"
     CDS             17808..18626
                     /locus_tag="LND56_000137"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6742091.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="LND56_000138"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="LND56_000138"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6742092.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="LND56_000139"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="LND56_000139"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6742093.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="LND56_000140"
     CDS             complement(20506..21087)
                     /locus_tag="LND56_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6742094.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="LND56_000141"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="LND56_000141"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK6742095.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="LND56_000142"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="LND56_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK6742096.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="LND56_000143"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="LND56_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK6742097.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="LND56_000144"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="LND56_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6742098.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="LND56_000145"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="LND56_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6742099.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="LND56_000146"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="LND56_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK6742100.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="LND56_000147"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="LND56_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK6742101.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="LND56_000148"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="LND56_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK6742102.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="LND56_000149"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="LND56_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK6742103.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="LND56_000150"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="LND56_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK6742104.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="LND56_000151"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="LND56_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK6742105.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="LND56_000152"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="LND56_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK6742106.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="LND56_000153"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="LND56_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK6742107.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="LND56_000154"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="LND56_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK6742108.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="LND56_000155"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="LND56_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK6742109.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="LND56_000156"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="LND56_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK6742110.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="LND56_000157"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="LND56_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK6742111.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="LND56_000158"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="LND56_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK6742112.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="LND56_000159"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="LND56_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK6742113.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="LND56_000160"
     CDS             33541..33726
                     /locus_tag="LND56_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBK6742114.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="LND56_000161"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="LND56_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK6742115.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="LND56_000162"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="LND56_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK6742116.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="LND56_000163"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="LND56_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK6742117.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="LND56_000164"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="LND56_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK6742118.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="LND56_000165"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="LND56_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK6742119.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="LND56_000166"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="LND56_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK6742120.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="LND56_000167"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="LND56_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK6742121.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="LND56_000168"
     CDS             37801..38448
                     /locus_tag="LND56_000168"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK6742122.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="LND56_000169"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="LND56_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK6742123.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="LND56_000170"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="LND56_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK6742124.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="LND56_000171"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="LND56_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK6742125.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="LND56_000172"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="LND56_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK6742126.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="LND56_000173"
     CDS             39886..40824
                     /locus_tag="LND56_000173"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK6742127.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="LND56_000174"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="LND56_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK6742128.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="LND56_000175"
     CDS             41504..42343
                     /locus_tag="LND56_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6742129.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="LND56_000176"
     CDS             42319..43188
                     /locus_tag="LND56_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6742130.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="LND56_000177"
     CDS             43178..43975
                     /locus_tag="LND56_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK6742131.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="LND56_000178"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="LND56_000178"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6742132.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="LND56_000179"
     CDS             complement(45257..47926)
                     /locus_tag="LND56_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBK6742133.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="LND56_000180"
     CDS             complement(48164..48352)
                     /locus_tag="LND56_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK6742134.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="LND56_000181"
     CDS             48622..48984
                     /locus_tag="LND56_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6742135.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="LND56_000182"
     CDS             49036..50718
                     /locus_tag="LND56_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBK6742136.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="LND56_000183"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="LND56_000183"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK6742137.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="LND56_000184"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="LND56_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK6742138.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="LND56_000185"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="LND56_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6742139.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="LND56_000186"
     CDS             54553..55557
                     /locus_tag="LND56_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742140.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="LND56_000187"
     CDS             55558..56502
                     /locus_tag="LND56_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742141.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="LND56_000188"
     CDS             56502..57464
                     /locus_tag="LND56_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742142.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="LND56_000189"
     CDS             57491..58405
                     /locus_tag="LND56_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742143.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="LND56_000190"
     CDS             58431..60212
                     /locus_tag="LND56_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6742144.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="LND56_000191"
     CDS             60560..61246
                     /locus_tag="LND56_000191"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK6742145.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="LND56_000192"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="LND56_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBK6742146.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="LND56_000193"
     CDS             64856..65665
                     /locus_tag="LND56_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6742147.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="LND56_000194"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="LND56_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK6742148.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="LND56_000195"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="LND56_000195"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBK6742149.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="LND56_000196"
     CDS             complement(69217..69669)
                     /locus_tag="LND56_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBK6742150.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="LND56_000197"
     CDS             69819..70769
                     /locus_tag="LND56_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6742151.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="LND56_000198"
     CDS             70762..71721
                     /locus_tag="LND56_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6742152.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="LND56_000199"
     CDS             71718..72473
                     /locus_tag="LND56_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6742153.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="LND56_000200"
     CDS             72496..73449
                     /locus_tag="LND56_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6742154.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74093..74389)
                     /locus_tag="LND56_000201"
     CDS             complement(74093..74389)
                     /locus_tag="LND56_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBK6742155.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74744..75100
                     /locus_tag="LND56_000202"
     CDS             74744..75100
                     /locus_tag="LND56_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6742156.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75110..76009
                     /locus_tag="LND56_000203"
     CDS             75110..76009
                     /locus_tag="LND56_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBK6742157.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        24831 a        13781 c        16342 g        21257 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacgatgttc
    73921 catctgctgc ttctcggcaa taagcccact agactagaaa atatgagaag ctattttctg
    73981 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt
    74041 caatcagctg accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat
    74101 gtgtctcagc aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa
    74161 gcttactcca ccgttttaat aattgataga aataactctc ttctgctttg attttatact
    74221 cgcttaatat caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca
    74281 atgctttggt tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga
    74341 tattttgtgt ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc
    74401 caaagaagtc ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac
    74461 aaaaagtaat tctttttcca atagttgtta aaggtttttt attttttata gtattttttg
    74521 ttatttaaga aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt
    74581 tcaagaaact gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat
    74641 aaacgaatga cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca
    74701 tctgatgaat aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac
    74761 aaaagaagca caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga
    74821 tatcgaaaac ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc
    74881 atttcgattg atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt
    74941 ggctgataca ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga
    75001 tccatccaca cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg
    75061 cttgagaaaa ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt
    75121 ggcgatcatc agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca
    75181 gctttggcaa gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc
    75241 taataaagta gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac
    75301 gacatttcat ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca
    75361 ttttccagac ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct
    75421 tttagggatg aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat
    75481 cgtacgaatg aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct
    75541 cgaggtcaat gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa
    75601 aaaacttgag gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca
    75661 atctgctcct tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg
    75721 agaattactt gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg
    75781 ctttgaagat aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta
    75841 tgaatggcag ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc
    75901 tatgaaatta cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca
    75961 acatttgcaa gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag
    76021 taatgtgtaa aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt
    76081 tatcgtggat atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat
    76141 gatttgtaac tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac
    76201 aaaaaaaaat a
//
LOCUS       DAFCLG010000003        69815 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFCLG010000003 DAFCLG010000000
VERSION     DAFCLG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.003"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            144..992
                     /locus_tag="LND56_000204"
     CDS             144..992
                     /locus_tag="LND56_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742158.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1163..1504)
                     /locus_tag="LND56_000205"
     CDS             complement(1163..1504)
                     /locus_tag="LND56_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBK6742159.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1590..2297
                     /locus_tag="LND56_000206"
     CDS             1590..2297
                     /locus_tag="LND56_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6742160.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2284..2607
                     /locus_tag="LND56_000207"
     CDS             2284..2607
                     /locus_tag="LND56_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6742161.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2696..3733)
                     /locus_tag="LND56_000208"
     CDS             complement(2696..3733)
                     /locus_tag="LND56_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6742162.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4180..5163)
                     /locus_tag="LND56_000209"
     CDS             complement(4180..5163)
                     /locus_tag="LND56_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK6742163.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5579..6349)
                     /locus_tag="LND56_000210"
     CDS             complement(5579..6349)
                     /locus_tag="LND56_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742164.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6342..7277)
                     /locus_tag="LND56_000211"
     CDS             complement(6342..7277)
                     /locus_tag="LND56_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742165.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7871..9520)
                     /locus_tag="LND56_000212"
     CDS             complement(7871..9520)
                     /locus_tag="LND56_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6742166.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9715..10611)
                     /locus_tag="LND56_000213"
     CDS             complement(9715..10611)
                     /locus_tag="LND56_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6742167.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10604..11284)
                     /locus_tag="LND56_000214"
     CDS             complement(10604..11284)
                     /locus_tag="LND56_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6742168.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11285..12067)
                     /locus_tag="LND56_000215"
     CDS             complement(11285..12067)
                     /locus_tag="LND56_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742169.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12060..12977)
                     /locus_tag="LND56_000216"
     CDS             complement(12060..12977)
                     /locus_tag="LND56_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6742170.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12980..13264)
                     /locus_tag="LND56_000217"
     CDS             complement(12980..13264)
                     /locus_tag="LND56_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742171.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(14092..15213)
                     /locus_tag="LND56_000218"
     CDS             complement(14092..15213)
                     /locus_tag="LND56_000218"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBK6742172.1"
                     /translation="MAKLKLQLEEEERVNQVSEELSEEIKDLCKKAKNKEIFSITDQI
                     KYARFKGIEISESDESIVQDILLYRTYYFKVTAYRKNFEKDINGKYIDLSFVKLADLA
                     KIDMYLRMILSRMIFELEHSLKTLIISSITNSLTEDGYKIVEDYDMYMQNKFIDSQRK
                     KGNTKTNEEILSEYTQASEKILDKVKGKFGYDFDFYNHHHDKISIWALLEVMTLGNLE
                     RFITFYFEEEHFGYLGLKTAQKLLKYVTNVRNASAHSRPLIYNIVEPYQFGRADSNLK
                     KRRPVIQLTQFATNAGLDSELIARALTNMKMNDIVATIYLHEKYVQTQKLKTTNFEAL
                     SDLLDRIKVNGKMYEKNHELVEVFHFFEKIVMEYGKLIT"
     gene            complement(15263..16879)
                     /locus_tag="LND56_000219"
     CDS             complement(15263..16879)
                     /locus_tag="LND56_000219"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK6742173.1"
                     /translation="MSNASEITAKLWAMANKLRGTMDASEYKNYILPFMFYRYLSENQ
                     DEYLEKNGLDEYYEVTDSEEKEDYLEEISRGIGYAIAPEYTWEQLVSKIENHQIKASD
                     FQDMLDSFNLNAKRNPVAEDDFANVFSDINLGDTRLGSNTNERAKALNDVVLMVNDFT
                     FKDDSGHDILGDVYEYLIGQFAANAGKKGGEFYTPHEVSQVLAKIVTKDAKADDTQFR
                     VYDPAMGSGSLLLTVKNELPDGNKQGSVDFYGQELNTTTYNLARMNLMMHGINYRNMN
                     LKRGDTLDADWPFAEKDGMQIPLKFDAVVANPPYSAKWDIKNVDREKDTRFKGYGVAP
                     ASKADYAFVLHGLYHLDKTGTMAIVLPHGVLFRGASEGRIRKNIIDNNLLDTVIGLPA
                     NLFYGTSIPTAVLVFKGRESRRTKDILFIDASSEFVKGKNQNKLSPENIDKIIETYEK
                     REDVEKYAHVATLDEIKENDYNLNIPRYVDTFEEEEVTPLPHLAQELADVKQEIEKST
                     DKLFEMLGELHGTTDEAQEELRKFMEMLGR"
     gene            complement(16895..18109)
                     /locus_tag="LND56_000220"
     CDS             complement(16895..18109)
                     /locus_tag="LND56_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK6742174.1"
                     /translation="MSNEITKEPEIRFDGYEGDWEERKVGEVFSERSERSGEGELISV
                     TINSGVVKASELDRKDNSSDDKSNYKKVEVGDIAYNSMRMWQGASGYSPYNGILSPAY
                     TVIIPKKDIDSKFFAYDFKRYDMIQTFKRNSQGLTSDTWNLKFPTLKSVKVMVPSIEE
                     QKQISTFFESLDTTITLHQRELDLLEQTKQTYLKKMFPKTGEDKPEIRFAGFTDAWEQ
                     RKLGEVATRVTRKNKKLESTLPLTISAQFGLVDQNTFFNKQIASKDVSGYYLLKKGEF
                     AYNKSYSNGYPWGAVKRLDKYDMGVLSTLYICFRPVDINSDFLASYYDTDRWYKEVSK
                     HAAEGARNHGLLNIAASDFFETELMIPKNNNEQEKIADFFKSLDTTITLHQRELDSLK
                     EMKKTLLQKMFV"
     gene            complement(18106..19107)
                     /locus_tag="LND56_000221"
     CDS             complement(18106..19107)
                     /locus_tag="LND56_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HBK6742175.1"
                     /translation="MNEIQFLIYEDSDKVEVLIREETIWATQKTISQLFDVGVPAISK
                     HLKNIFESGELDEEVVVSILENTTQHGAIDGKTQTNKVKYYNLDAIISIGYRVNSQKA
                     TKFRIWATDILKDYIQKGFKIDVERMKQGENAFGKDYFRELLETVRSIRASERRIWQQ
                     ITDVFAEISYDYDKNSEITKQFYATVQNKFHYAITGKTAAEIVFNGADKTKENMGLTT
                     WKNSPDGRILQSDVSVAKNYLSEKQIRSLERNVSSYFDYVERLLEDETLLGMEDFAKS
                     IDEFLTFNRYQILDGNGKISSKAAKRKAVDEYKEFNKHQHIVSDFDKEITKIEGELK"
     gene            complement(19114..22188)
                     /locus_tag="LND56_000222"
     CDS             complement(19114..22188)
                     /locus_tag="LND56_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBK6742176.1"
                     /translation="MTKQFGIKSQSELRFEQEVVDYLTRIGGVKQWEYKAEIKTTEQL
                     WNNFKKILEQNNQARLDEPLSATEFNQVKKIITNINTPYQAGQFLYGVNGVSEIEVDL
                     DNGKHVFLTVFDQAQVGGGNTVYQVVNQIQRPKVVDGKPNRRFDITLLINGLPIIQIE
                     LKKALHNATEALNQMEQYIAEKQFSGIFSTLQILIAMTPFDIRYMANTTLQNFNRAFA
                     FNWQDEETAHPVRSWKLFADKVLSIPMAHDLSTRYMVLDGTKNKESIKVMRPYQVYAT
                     KRVLDKVRKFDFKHDEGKLGYIWHTTGSGKTITSFKTAWLASRLSNVDKVIFLVDRIA
                     LTNQTADAYRAYDPIAGFEGKTGVVSDTANISDLHRKLTKKSDKNIIVTSIQKMSRYV
                     TRDSFQPLTENVLFIVDEAHRSTGDGTKNEGMLESIRKALPNSAWVGYTGTPKFPETR
                     EIFGDLLHAYTIKEAIADKNVLGFKVEFKETIEAPADPTEEDIDDNLRGSVYDHSPEH
                     VELVVEDIFDNWKERSNNRKYNALFTVHVGGNRASTPRAMEYFDKFMEENNKRSIEDQ
                     IKVAISFSMDTSNSSHQLKTNENLHRAIQSYNRLFGTAFDMTTVKSYTEDLARRLNKT
                     ADDGNYLDLVIVVDQLLTGFDAPELNTLYIDRTLKGGNLIQAYSRTNRIHDRDAKPQG
                     NIVNYRWPIQNEYEMNKSFAIYSNRDSAAEQLSLDELKKDNEESGILSKSFSKIESET
                     KEVIQKLAELTDNFIQLPPSEKAQEEVFEGLQEYNRLLNQLKQFTEDDEKNPVSVYDE
                     PELFYDRLGITEDQEVMLTTVLANELRERRAQIENIDISQVNLEMTHIHDVTINYDYL
                     IELIARMADEVHEDKMDQAELTKNEIDVEIAKSDSDKEITKVRNFVSKIFTKEFIFDK
                     YPAPRDVEEMNQAMEQSQKDSNIKLYTEFVRTWGLDNSVKPKDLAKLISRHRVGQEDM
                     DKQGELTAMMNDARTDYKEIAAAEVANLSWVKYRIEFRKAFYQMADETKKGE"
     gene            complement(22192..22770)
                     /locus_tag="LND56_000223"
     CDS             complement(22192..22770)
                     /locus_tag="LND56_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK6742177.1"
                     /translation="MEMRKLSEFVTFVQGINQSRAEKQFGNQEIDYYDQASFEEDHKY
                     YNEATQEERVNYLFDQEVSLDKRDVVISNSLQRATMVSGQNVGKVLSLNFTKVEFKSD
                     LLDKRYFLYLFNNHKDIQRQKDRGLQGTGPVLRLTKQSLEQLVIPLPLIEEQRKIGGI
                     YTESLKLQSKLSIYTRLLEQFTNSMLEKSLEE"
     gene            complement(23073..23318)
                     /locus_tag="LND56_000224"
     CDS             complement(23073..23318)
                     /locus_tag="LND56_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6742178.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRIRLEKGIGQTELIRDLQLHKIA
                     ITRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            23423..24196
                     /locus_tag="LND56_000225"
     CDS             23423..24196
                     /locus_tag="LND56_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742179.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSETSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAKLYTEKVEQFFKSGANKDFSKISDYSENFFNETQDEIMNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLEKPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
     gene            complement(24426..24629)
                     /locus_tag="LND56_000226"
     CDS             complement(24426..24629)
                     /locus_tag="LND56_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HBK6742180.1"
                     /translation="MLQEVVHSNSEIEITRDSKNGLNDGVIMISKRKWELLQEELYLQ
                     RTGTWAYVSDLMKNEKEDDFKVV"
     gene            24710..25836
                     /locus_tag="LND56_000227"
     CDS             join(24710..24938,24938..25836)
                     /locus_tag="LND56_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6742181.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNTLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(26024..26374)
                     /locus_tag="LND56_000228"
     CDS             complement(26024..26374)
                     /locus_tag="LND56_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742182.1"
                     /translation="MIFENESEEIGRMTEENKVDLLETLVINKKEFEQLFLDFFVQYD
                     RIYDFNSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDADYYQPQEVFR
                     EFRNKIIGFSQTTK"
     gene            complement(26415..26909)
                     /locus_tag="LND56_000229"
     CDS             complement(26415..26909)
                     /locus_tag="LND56_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742183.1"
                     /translation="MFKVYFPFQWLVKGIFFVLLGLILFGSYLTIKARYFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKSETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(26902..27204)
                     /locus_tag="LND56_000230"
     CDS             complement(26902..27204)
                     /locus_tag="LND56_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742184.1"
                     /translation="MSDPYIKVYCSKETKKLFDSFYEKNKGTLGTKANMFRVMVSNLP
                     MLASQSNKKFSDPESIKLEQTISELESSIMKKLDGIDQKLSCSLKTKYKTEEKKDV"
     gene            complement(27227..28564)
                     /locus_tag="LND56_000231"
     CDS             complement(27227..28564)
                     /locus_tag="LND56_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6742185.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     EFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQRTGKKMDIRKNDIRVLQAWNDNILIDNGINPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKNHSIETYRFAKNQ
                     RIGYKLLDSEDGLILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSEEVLTEIAIRYL
                     GKSLNGRADSRFNWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIENLLDV
                     FGENKNQFFGFAVEEEPLKYKKKCVNLVYKKVVYDIAESLSTDYDQFNIQDTEPALKR
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETARGIDYQKQQNQYDMDF"
     gene            complement(28549..29046)
                     /locus_tag="LND56_000232"
     CDS             complement(28549..29046)
                     /locus_tag="LND56_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742186.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAEKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            complement(29195..29533)
                     /locus_tag="LND56_000233"
     CDS             complement(29195..29533)
                     /locus_tag="LND56_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742187.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKYVYEKTGISSVKEFE
                     ETFEINQKGTQEKWQKAVNFANSEFTLVHEFLASDQLMETKFQQFLRRKGETAYRIFY
                     REEAQEESVR"
     gene            complement(29537..29722)
                     /locus_tag="LND56_000234"
     CDS             complement(29537..29722)
                     /locus_tag="LND56_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742188.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     VMKNELMNELSHIQKGA"
     gene            complement(29736..32489)
                     /locus_tag="LND56_000235"
     CDS             complement(29736..32489)
                     /locus_tag="LND56_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742189.1"
                     /translation="MKIIKKKIDNRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQSVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMINKT
                     NDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVATKFSDN
                     FLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLNAGEGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERRLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTMSAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENPQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDV
                     RENLQNTTSSQNGNRTVDVRENVQSSGTSQPRTVDIRENLQNTTSSQNGNRTVDVREN
                     VQSSASNQPRTIDVRENLQNTTSSQSGNRTVDVRENVQGSASNQPRTVEVRETRAEAS
                     QGDKTQKINIVEERKERPNEKVIVEQEVKEKKRQRFTYENNPLFQDISDKPNPLFDSL
                     FKK"
     gene            complement(32476..32694)
                     /locus_tag="LND56_000236"
     CDS             complement(32476..32694)
                     /locus_tag="LND56_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742190.1"
                     /translation="MIENHLVDKIYCLDHNADTLELIESICSNTIVLDLRAVNDDYVT
                     HIIVNKKMAQKLSESLKKWAEGGNNENN"
     gene            complement(32687..33427)
                     /locus_tag="LND56_000237"
     CDS             complement(32687..33427)
                     /locus_tag="LND56_000237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742191.1"
                     /translation="MREKLKKLGDKKRHTFQGTFEFNGYKKDKYKFGQEYENATFMLS
                     NLKIVQGDNLELVTDHIWMNLTKQFLKFGWLKKGDMVQFDGRIKSYSSKKGINYKIER
                     PSKVKVFRKGTEINESSALKISTRYEIVEDIKRQNREYYEARDFFFENYFSVPRYFKL
                     KEKWPEIQAVVAINENRYTSEQFKQVFKDRDSLVDLYKKIQETDKYNLQKEFYNGLVS
                     NKNRIDLNEVEGYLHKIENFDYGRGYFD"
     gene            complement(33560..34435)
                     /locus_tag="LND56_000238"
     CDS             complement(33560..34435)
                     /locus_tag="LND56_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HBK6742192.1"
                     /translation="MAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYTKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPIKFDMILQSGQVEPLRVDLMEKWYPAGIPCDVKSDTQLSQDQNSQDQ
                     TNQDKDSIEEINQDQNSFFENIINQKGIQIITRLGDRYTEVQREKYLKLIASMKDSIE
                     REYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEEIDEMKFINTTARNHWEDIAKQE
                     KASRLDMLETLKKIG"
     gene            complement(34605..34886)
                     /locus_tag="LND56_000239"
     CDS             complement(34605..34886)
                     /locus_tag="LND56_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742193.1"
                     /translation="MEQIGVSVMLNTDQTNQLKTHVFEVTKEAIDQARIDSGLERPFL
                     KGKEMAKFLNVSYGTFLKFKRLGLPIIVVDTIELYSKEEVKKWMLQHQI"
     gene            complement(34941..35138)
                     /locus_tag="LND56_000240"
     CDS             complement(34941..35138)
                     /locus_tag="LND56_000240"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6742194.1"
                     /translation="MFQINEDFSIRIRERRGKLMITKTKAAELIGISRQTLKKIECFE
                     VQSVSKNVYQKIIGWLLKSDY"
     gene            35305..35679
                     /locus_tag="LND56_000241"
     CDS             35305..35679
                     /locus_tag="LND56_000241"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6742195.1"
                     /translation="MTQKNRIKETREEQNLTLKELSNLSDIPYQTLRNYEIGKRKPRN
                     DEVWWKLGTILNVRGPYLKGEDSFKTNDELHIYYLKKSHTNFELSKKEEIIDIIDKLN
                     EDGLDEALRQLALLSKIDDFKR"
     gene            35769..36935
                     /locus_tag="LND56_000242"
     CDS             35769..36935
                     /locus_tag="LND56_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6742196.1"
                     /translation="MSIQKYKTKSGTRYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDMGYHRPSNDLFRTVYEEWLPSYKNRVQETTYQRTKDLFRLHILPKFG
                     DKSISKITPIYCQNAINEWAVKYTNIKQLKSYSSQIFEYAIFAELLQRNPMTNTKLPK
                     QEKKESENYFSLEELKEFLEILNKEESLKHILIFQLLITTGVRKGELSALRWADINFE
                     EQLLHIGKSYATIRTEDPNATRKTIRIQKNTKNVSSERILPIDTQTIEMLKLWKKEQA
                     EVLLQFGINANTKKQLIFTYVNANGDVNQPLHADYSNNIMRRLEKKYKFKHVTIHGLR
                     HTHATLLLEGGASIKEAQDRLGHKNAETTLNTYSHVTEKAQRNAVDKFIAYTGL"
     gene            complement(37034..37426)
                     /gene="rpsI"
                     /locus_tag="LND56_000243"
     CDS             complement(37034..37426)
                     /gene="rpsI"
                     /locus_tag="LND56_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6742197.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(37442..37885)
                     /gene="rplM"
                     /locus_tag="LND56_000244"
     CDS             complement(37442..37885)
                     /gene="rplM"
                     /locus_tag="LND56_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK6742198.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(38404..38871)
                     /locus_tag="LND56_000245"
     CDS             complement(38404..38871)
                     /locus_tag="LND56_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK6742199.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(38991..40529)
                     /locus_tag="LND56_000246"
     CDS             complement(38991..40529)
                     /locus_tag="LND56_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK6742200.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            40609..41295
                     /locus_tag="LND56_000247"
     CDS             40609..41295
                     /locus_tag="LND56_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK6742201.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(41496..42023)
                     /gene="msrA"
                     /locus_tag="LND56_000248"
     CDS             complement(41496..42023)
                     /gene="msrA"
                     /locus_tag="LND56_000248"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6742202.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(42107..42784)
                     /gene="pgmB"
                     /locus_tag="LND56_000249"
     CDS             complement(42107..42784)
                     /gene="pgmB"
                     /locus_tag="LND56_000249"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6742203.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(42781..43911)
                     /locus_tag="LND56_000250"
     CDS             complement(42781..43911)
                     /locus_tag="LND56_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6742204.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(43911..46094)
                     /locus_tag="LND56_000251"
     CDS             complement(43911..46094)
                     /locus_tag="LND56_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6742205.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(46091..47116)
                     /locus_tag="LND56_000252"
     CDS             complement(46091..47116)
                     /locus_tag="LND56_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6742206.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(47161..47988)
                     /locus_tag="LND56_000253"
     CDS             complement(47161..47988)
                     /locus_tag="LND56_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6742207.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(47985..49052)
                     /locus_tag="LND56_000254"
     CDS             complement(47985..49052)
                     /locus_tag="LND56_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK6742208.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(49078..49914)
                     /locus_tag="LND56_000255"
     CDS             complement(49078..49914)
                     /locus_tag="LND56_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6742209.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(49911..50780)
                     /locus_tag="LND56_000256"
     CDS             complement(49911..50780)
                     /locus_tag="LND56_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6742210.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(50850..52133)
                     /locus_tag="LND56_000257"
     CDS             complement(50850..52133)
                     /locus_tag="LND56_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6742211.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(52147..53832)
                     /locus_tag="LND56_000258"
     CDS             complement(52147..53832)
                     /locus_tag="LND56_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBK6742212.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            54107..55132
                     /locus_tag="LND56_000259"
     CDS             54107..55132
                     /locus_tag="LND56_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6742213.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(55727..59401)
                     /gene="rpoC"
                     /locus_tag="LND56_000260"
     CDS             complement(55727..59401)
                     /gene="rpoC"
                     /locus_tag="LND56_000260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK6742214.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(59459..63076)
                     /gene="rpoB"
                     /locus_tag="LND56_000261"
     CDS             complement(59459..63076)
                     /gene="rpoB"
                     /locus_tag="LND56_000261"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6742215.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            63804..64772
                     /locus_tag="LND56_000262"
     CDS             63804..64772
                     /locus_tag="LND56_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6742216.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            64790..65695
                     /locus_tag="LND56_000263"
     CDS             64790..65695
                     /locus_tag="LND56_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742217.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            65692..66498
                     /locus_tag="LND56_000264"
     CDS             65692..66498
                     /locus_tag="LND56_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6742218.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            66634..67623
                     /locus_tag="LND56_000265"
     CDS             66634..67623
                     /locus_tag="LND56_000265"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBK6742219.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(67940..68464)
                     /locus_tag="LND56_000266"
     CDS             complement(67940..68464)
                     /locus_tag="LND56_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBK6742220.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            68751..>69815
                     /gene="scm"
                     /locus_tag="LND56_000267"
     CDS             68751..>69815
                     /gene="scm"
                     /locus_tag="LND56_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6742221.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        20314 a        14579 c        11874 g        23048 t
ORIGIN      
        1 cacattctct aagaaaagaa aaggagaatg aatttttgac aattcgagca aaagtgatta
       61 tcaaatatgt aaaatcagct acttttttct taaacgtttc tttatactaa atataaataa
      121 gtgtttcatg aaaggagaac tagatgaata attggcttga gtattttcct gaaaacgttt
      181 tggaacgcgg ttacagctat catctgcacg gttttgtccg tcatttgaac tatacaagca
      241 agtacttgtc cgcaacagtt tctggcacgg aagattataa ggtagtgatc acttgggatg
      301 aaaagaccaa catgacttgt gattgtctat atgccataga aggaaaaaaa tgcaaacaca
      361 tggctgctgt cttattcgct tacgaagaac gtccaataaa aaaatccaac tactcacttt
      421 ccgaactctc gtcccttgtt tcctccgcta gttcctctct tgtacgggag cttttaacag
      481 agattcttat agaacatccg cactttatcg aacgcttcaa agttaagatg ccatttcatg
      541 caataaatta ttcagataaa ttaacaacta tcatccataa atacgatcat attatcaaga
      601 aaaacaaaaa caggaaaaca gcgaagttca tcatggagat gcgaaagttc atccaagaag
      661 cagttgaatc gcttattcag caaaatgcct atcttcccgc attcgaattg atcaatgaag
      721 tgattgctac tctcgaaaca ttctattggg agcctgagga tgaacgaacc cttcttctga
      781 tagaggactg ttattattta tggaaagaat tattagcaga agcacctcat gcagaaaaaa
      841 ggcaaatgtt ctcttggttc gtctgccaag tcgatcatac tgacgcctct tattcaaaac
      901 gctactctat aaaaatcctg aaagaagact ttcgggaaaa agaattttct aaccaaaaga
      961 aaaaaataga taaaaaacta aagaaacggt aaagaaagac gatttcaatg aaaaatagca
     1021 agtaaacgat aacaaccatc ctaaatccac aagaaataca gcagaccttg ctttaaaaac
     1081 acgaatcatg caaaggatga agtaaagaag acttaacgcg tacccaatat tagtcatttg
     1141 actattaggt acgcgttttg ttttataagt catgacaaat ttccttcgtt gctggataga
     1201 ttttccggca ttgttgatag attttatgat acttttctac attttgtaag agaggttccg
     1261 ctgcatctgt atattggata aaattctgac aacaactttc aggtgtgtcg aaccacccta
     1321 accctagtgc agcaatcatt gttgcgccta atgcaggtcc ttcctccata cttaaacaat
     1381 agaccgttgc atcgaaaata tcagcttgga tttgtagcca tgcaagattt ttcgcgcctc
     1441 ctcccaccga aacaatacgt gtaaatctgc gacccgctag ttctttacgt ttcaatccca
     1501 ccattgacaa atgctcatta aaaatttaga atattcacaa gtttaacgac aattatgaaa
     1561 atttatgtcg tgctaaagga gttttgttat tggatgaaaa gttagatata aaaactgcga
     1621 tcaaagacac gttacctacc gttttcggtt atatcggtat tggacttgca tttggtatcg
     1681 ttgggaaagc tgccggattt catccactag tcgtcacgtt gatgtccctg ctggtctatg
     1741 ctggttctgc ccaatttatc acagtcagca tgcttgctag tcacagccca ttgctttcca
     1801 tcgttttctc gacctttcta gtcaattccc gaatgattct gatgagtatg acgattgctc
     1861 cttatttcaa gaaaaatcgg ctgcttcaaa atctgctgat tggcacattg ctgacagatg
     1921 aaagtttcgc actgggaatg aataaattga actatactgg acaaaagtta aactttcgtt
     1981 ggatgaatac agctaattgg atttcttatt tgacgtgggt gggttcttct cttgttggcg
     2041 cacttttagg gaattttatt actgatccga aaaagtttgg tttggatttc gccatcgtcg
     2101 ctatgtttat cgggttactt tatcttcaag tcatttctga tagaaacacc tctaaacgat
     2161 tacagttgat cctgattggt ctcactttga ttctggtata tgtcggactg atttttatcc
     2221 caagcaactt agtgattgta gttgtaacac tgattgggtg cggattgggg gtgtggatca
     2281 aacatgcctt cttttgagtt tacattgctg actattttag gttgtacttt agcaacttgg
     2341 ctttctaggg tcctaccatt tgtcttactg aaaaaattcg atttaccgca accgttgctt
     2401 gagtatttaa gttttgtccc gatcgtcatt atgtctgctt tatggttcag cagtctattt
     2461 acccaaaata tcggacatct gccgcagatc aatctcgaaa atgctttggc ttcagttcct
     2521 acacttttag ctgctattct ttcaaaaagc ttattggtga tcgtcttggc aggaattctt
     2581 tccctttcgt tgattcgctt attataaata aaaaaacaag agcttatggc aaattactgt
     2641 catgcgctct tgtttttatc aaagtcaaat aagaaaaaaa tcggcgtatt cttgcttatc
     2701 cgttgaacgg accactaaca acgacttttc ctaacatatg tcctgtttct acgtctttgg
     2761 ttgcttgttt caagttcttc gcattcatcc catctgaata gaccttgctc aatgtactgc
     2821 ggataatccc ttcatttgct aaagcagcaa tgtgcgctaa agcttctcct tgcgtttcga
     2881 tttcatagtt atagtctgtt ttagcgaaca tatactccca gtcgaagctg atagattggt
     2941 ttttccaaac acctatatct aacggctctt tgatccctac gatcgttccc acatgtccaa
     3001 atggtcggac aaggtgcttg atttgatcaa agtagcgggt aatatcgaat aatacagcga
     3061 catactctac ttgatcgatt cctaattcac gaaaactttt acttaagtcg tgatgataat
     3121 cgattggata gtctacacct gtttttttca gccattcaaa attcttcgga cttgcagtcg
     3181 cgtagacttc taggccagcc caatgcgcta gttgattcaa aatcgaaccg acgcccccgg
     3241 atccgttgat cactaagatt tttttcccgc gattggcatt ttcttctgga gttagaccaa
     3301 atttttcaaa taacaattca taagcggtca atgaggtcaa cggcaaggct gcagcttctt
     3361 catctgaaag attttttggt gcaagagcga caatacgttc gtcaactagt tggtattctt
     3421 gattgcttcc tgctcttgtt gttgtacctg cgtaaaaaac gcgatcgccg acagaaaatt
     3481 tttgcacttc gtcacctaca gcaacaactt tcccgactcc atcaaaacct aagatacgta
     3541 aagcatttct tatcccgtta tcttggcgaa gtttcgtatc gacaggatta acagatactg
     3601 cgttgacttt taccagaaga tcccgtgaac tgggtacagg tatctgttct ttttcatcaa
     3661 taaagctttt tggatcatcg atcggcaacc cttcataaaa accgactacc ttcattaatt
     3721 ccttttctga catacgaatt cctccttagc ttcatcaagt tggagcatcc atacagtgtt
     3781 catttcaata tatatagtat gctatttgtt ccaccttttt tattcatcat aattattctc
     3841 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     3901 gtcacaacct cttataaacg gtgttctcct accaactctt cggtcacaag tcaaaatatt
     3961 catcaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
     4021 gctcacaacc tctgatataa acggtgttca atcaactgac cctcggtatt agctcaaaaa
     4081 acgacaaaat atgaagagct atttttgttt ggctgttctt attacttgat acaggacagc
     4141 tttttcacga cctctaattc acggtgttca agcaagaaat cattgttcac tgaaaagcgt
     4201 tggatgttct ttttcaaagg tctctaagat ctctcgtact tcatcagtag atttcgtatt
     4261 catcaactga actcgcaagt catttgctcc tggaaatcct ttgatataga ttttaaagaa
     4321 acgatgtagt cctgtgatgg aacgcgggat gatttctgaa taatgatctt gcagatctag
     4381 ttgcagacga agtagtccga tcagttcttt ttctgtatgt tccctcgggt ctttttcaaa
     4441 tgcataagga ttcttgaaaa tccctcggcc aatcatgatg ccatcaacgc catatttctc
     4501 ggccaattct tctcctgtct ggcgatcggg gatatctcca ttgatcgtga ttagtgtttg
     4561 tggagcgatt tggtcacgta aagcaatgat tttaggaatg acttcccaat gtgcatctac
     4621 tttactcatc tctttacgcg tacgcaaatg gacagaaaga ttggctatat cttgatgaag
     4681 taggtgactg atccattctt ccatctcaga catttccgta tagccgatac gcgtcttgac
     4741 gctgacaggc aaaccgccag ctttagctgc ttcaatcaat tgtgccgcta cttctggacg
     4801 aaggatcaag ccactgcctt ttccacgttc tgctacatta gggactgggc agcccatgtt
     4861 gatatcgatt ccttggaagc ccatttctgc catagcaatg ctcatttctc taaaaaattc
     4921 tggcttgtct ccccaaatat gggcaaccat cggctgttca tcttctgtaa atgttaatct
     4981 tcctcgtaca ctgtcttttc catctggatg acagaaactg tctgagtttg taaactcagt
     5041 aaagaagaca tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa catctgttac
     5101 atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa aattagtagt
     5161 catttatttg ttactctcca ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat
     5221 tgtcattata ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag atgtttctga
     5281 ttgagcaaaa aaaagactgt gacaaaaatc ttatcacagt ccattttccg gaataaatgg
     5341 tgttcaaaag cttgcatttg cggtaataag tccaaccaaa cgaaaaatag gaggagctat
     5401 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa
     5461 acggtgttca atcagctgac cctcggtatt cgctcaaaaa acaacaaaat atgaggaact
     5521 attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct
     5581 aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga
     5641 tgaataacac aagtacgatc atcgatgttg ctactggcac atcgatcgtt ccaaggaatc
     5701 cgtaacggaa accggagatc atataaacga tcggattgac ttttgaaaca gcttgccaaa
     5761 agggcggtaa catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga
     5821 caaatgtagg cacgatcgat acatcatcat aagactgagc aaaaatcccg ttgagcagtc
     5881 ctgctaatga aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat
     5941 acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac
     6001 tgcgacccac accaccaatc acaaagcccc aaataatcac atgcgtagga acaggtgcta
     6061 ccaagatttc ttcgatattt ttctgaaatt tttgagagaa gaaggacgaa gaaacattgg
     6121 catacgaact agtgatagca gacatcataa tcaaaccagg aacgatgaat tccatatatg
     6181 aaaaaccacc catgtcaccg atccgcccac cgatcatttt tccgaaaata acaaaataca
     6241 aggatgtcgt gatcactggc ggaaccagtg tttggaccca tatacgcaaa tagcggttcg
     6301 tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc
     6361 gtatgattat tttcttctgt gattttcaag aacagttctt ctaaacgatt cgacttattc
     6421 ctcatagaaa gaactttgat tccttgtctt gtaagctgat cgaagattcc gttcacccct
     6481 tgatttcgtt caacttcgac agcgattgtc tgctcatctt caaatgtaca agcatatcct
     6541 tcaatcacag gtttggtatc atatggcgct aaatcaaaga taaatgtttc aaattgcagt
     6601 tttgacagaa gctctttcat acttgtattc tcgatcagtt cacccgattg gatgattccg
     6661 atgttgcgac aaagcatttc agcttcttct agataatgcg tcgtcaaaat gatcgttgta
     6721 ccattttcgt tcaattctcg tagaaactcc cacatctcgc gtctcaactc gatgtcaact
     6781 cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc
     6841 atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa
     6901 agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg
     6961 tagtagcctg cttgattcac gacgatttgc tggaccgttt caaaagggtt aaaattgaac
     7021 tcttgtggaa ccaatccaat ctgttgcttt gctcgaacta gatccttgtc cagatcataa
     7081 ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaataat cccgatcgtt
     7141 gtagacttcc cagctccatt tggccctaat agcgcataga aatctccttc tttcaccgaa
     7201 agatcgattc cccgcaatgc ttcgacccca cttccataga tctttttcaa attatttatt
     7261 tcaagcgcat ttgccattta tttcctatct ccttatagac atgattctta attgtatcat
     7321 ggaatacagc agatgaaaaa acaacttggc ttccacacaa aaacacacct gctaacaaag
     7381 aggagatgtg ttttctagtt catgatcatc ttcgatagaa taactatcta aacaactctg
     7441 gaacttttaa aatcataaaa gtaccgacag caactactac gatgacatat agaagatcct
     7501 tataactgag gttcttgtct tttttattca tgttaagctt tcctccgtcc ttttatcgaa
     7561 agcttttttc ttttatttta accgaaataa ataaaatgga aatagttatt ttcaatttct
     7621 gttttcgctg tttgttttac ggagaacttt gccatggaac gtgcgtctcg tcgctgatgc
     7681 taagtttttt cgataaaagc tgctagaagg aagacagcgt ggtttatttt gtatcgtctt
     7741 ttgctaaatc ttccaagatc atctggacag cagactcgtt cccattgaat tctgccactt
     7801 ttttggcatc aatgtacttc ttttgataat cgtctttttg gacccacgca tcatgcttcg
     7861 tttgataaag ttaatcaaac ggataccact gacgctgttc atcgaacaac aaactttctt
     7921 tttgggcctg ttcaactatg gatttaggat aattcaaaac tttcagcaag gcaatcgcat
     7981 ttcgggtcct tgaaggtcct tcttttaatt tgaaatcaaa actgattcct tttccttctg
     8041 ttacttgttc ttcaaagtgg acattctcac aataattctt taaaatctct gtcaattcga
     8101 tatcatgtgt ggcgacaaat gctaaagatg gatactccgc aagccaccga acgactgaag
     8161 cggaagaagc aatccgttcg atcgtatttg ttccttttaa aatctcatct acgaagcagt
     8221 agcagcgttc tttggaagcc acttggtcca gcaaacgttt gatcgatttg atttctgaaa
     8281 caaaataact gtcgccttca aataaatcat cttcgattgc catggaagtc aacacatgtc
     8341 caggttgcag ggtaaactgt tctgcaatcg ctgtttggat cgtctgtgct agaatacagc
     8401 tgatggctat acttttgaca tacgtagatt ttcctgatgc attggaaccc gtgactaatg
     8461 tattcttctg ccagttgact ggattaacta ccgcttcttt taacaatgga tgatagatat
     8521 ctttcgctgt cacaccgccc tcagtaaact ctggtcggca agtgatcggc atataggtgc
     8581 gataattcaa aacggctgct gcaacttcca attctcccag caattcccac agcttgatgg
     8641 cttcagttga atgcttttct aatttagtga gaacgaaatg ataggagata aatggcagca
     8701 tgaacatgat gttgacatat tcaaaaatca attctgcttc actcccgctc ttcattcgaa
     8761 atgaaaaacc taatttaggg atgtctctta atggtttagt ttgctgtttc agctcttttt
     8821 gaagtggtgt cgtcattttt gctaactgat tggcggctga aatcgttgaa accaaataac
     8881 tcatgctatt cagctctgtt tctagtgttt gtttcttcac tagataatag accgtattga
     8941 atactaagcc accgattgct agatagattc ctagtacttg ccctaaaaga agcaaaccta
     9001 cacccaccag cggaagcaag ccaagaaaaa taaataaacc tacgcgtcca atctttttac
     9061 tttttccatc cgctagatat tgttttgcaa aattatgatc tttttttcct aaacgagcga
     9121 attgatattg gattctttct ctcagttgag gattttcctg ataaaaagca attagttttt
     9181 ctaactgata atcttctcca aaatcaaagc tacgtaaacg ctgatacaac gcttctgaac
     9241 ctacactaga ataggtactg ttgatccctt caaatacttc gaacatatcc aagtcatacc
     9301 aagtaagatc gtcgatttca ctatcccatg aacggaattt tttctccgtg agccacgcat
     9361 ccttcaaact ttcttcttta tcaaatctcg gttgataagg aatttttccc caattactac
     9421 gaactgtttc ctttactttc aaacgtgaaa aaatgtctaa aacaactaat aaaataacaa
     9481 ctgcaataat aattaaaacg ataaattctc tttggcccat atccctctcc gttccttgat
     9541 ttgttagttt tagtatagca ctgttgcgct atttgaaaag ggggtacaaa aataactgtc
     9601 ttcgttacct aattcgtatc aatcacacca aaataaaaac accttccaaa aaagaagatg
     9661 tctttaaaac gagatacaat tttttgtcat tcacttcagg taaattagag ccaattaaac
     9721 cctccgcaac ttgacggaga tggagaagat gcccccgtct aatctagcgg tcatctctcc
     9781 tcccattcct tccaccaatt tttttgacag gtaaagcccg attccgctag attccgtatt
     9841 tgtgcggctg gtattttcgg tataaaaacg ctggaagact ttttcaacag aggtttgcgc
     9901 tcctggtttg ataggattgg ccactgttaa tcgaatgaat gaatcctctt ttgaaaatgt
     9961 cacctttgct tgtccatctg cataacgcaa taaattctgt atgatatttt gcagcactcg
    10021 cagcaacatg agcggatctg ctaatacagt tgtatttatc gagtgttcag cgaacgctac
    10081 ctcgatattt ttctcagaaa attgttcata aaaagagaaa aatacttctt ccacctcttt
    10141 agaaaaagaa agcgtatgga gatccatttc tttttgcttc gtctctattc gagcaagatc
    10201 ataaaactga tccgtcatac tgagtaatct tgcaacagat tgtttgatga tcgttaaata
    10261 cttctcccgt ttctccgtat cctctacctg ttccagtaat tgtacataac cgttaatagc
    10321 agtcaaagga gttctgagat catgagaaag gccgacgatc gatagtctga tgttttcttc
    10381 catttcttga aatgcttgtc ctttttgttc attttcatcc acccatgtat tcagtgcatc
    10441 tactaggtcc attagattct tttctcgaaa ttcgactgaa agacgaccac catattcttg
    10501 tcgttcagaa agagtggcga ttttcttagt taatctcttg atttcccttt gcaggaagaa
    10561 aataaaactt cccataaaga taacaacgag aagcaacagc cacttaatca aatctaaacc
    10621 ccactcccca caccgtatga atatacacag catgtgcctt tgctagtttc cctcgaagat
    10681 tggaaatatg catattcact gttttctcac tatcaaaata tggttcttgc caaactgttt
    10741 cataaagatt tgctcgcgaa aatacttttt tcggatgttc taaaaaaagc ttcagcagat
    10801 cgaattctct tcctgtcaaa tggacagcca caccattcac atttacatcc ctcgactctt
    10861 tgtccagtaa gatttccttg taatagatgc gttgcgacag atcttttact tggacagaag
    10921 tctgatgacg aagttggatg ccgattcgta acaacagttc ttcgatatca aatggtttcg
    10981 tcacatagtc atccgctcct gctgccaaca aggaaacttt atctctttga tcgctttttg
    11041 ctgaaagaac gatgatcgga acattcgatg tctgtcggat ttctgctaac aatgcttcac
    11101 ctgaaagccc tggaagcatg agatctagaa ggatcaaatc gacaggatat gtgcttaaca
    11161 ataattttcc ttccgtacct gaataagcat caagaagttt gtatctctgt tcaagaacct
    11221 cctccagtaa agaatgaaca gcttcatcgt cttcgataat caaaatagtt tccagcagga
    11281 acacctactt aatgtcagat ttttggaaaa taaacattcc tagagctgat gataccacga
    11341 ttgttactag actgaccaga atcattttcg gtgcttgcga ataagttgct tgttgcgcaa
    11401 agtccgtata catgagccac gtttccgtaa accactccgt tagaaattcc aagcctttga
    11461 tttttgcgaa aacaccggga acgaaggccg ttcccatgat ataaagaaaa gaaagaagca
    11521 aactgacact ggtacttcta gtgatgaatg tcaaagccac tggaacagat aatgtcgcta
    11581 atatcagcag aagcaataca ggaaatactt ttagaacata atttccatag tttttgattc
    11641 cctcaacttc accaaatccg aaagcaagtg tgtaagcaat catggctagc cccgtaatca
    11701 aaaccacgcc aagtgttgtg atcaaaagcg taacgagaag tttcgacaca taataagtca
    11761 ttcgattccg tcctaaagat aacgtattgc gaatcgtacc gttttgaaat tcttccccaa
    11821 tataaaagca cgcaaatgct gcgaaagcca gaagaatcca tgcagccatt ttttccacca
    11881 ttaaaatccc tgttgcaccg ttgatgactg tatccgttgc ttggtttaat tcattgccgt
    11941 attttgtatg catataccat tcgttccaag aataaaaaag cggaagtacg accatcagtc
    12001 ctagtgagac ccacatttta cggctcttcc atacttttat tttttcagca gttaatagat
    12061 taaacatgtg gattgcctcc agttaattgt aagaaataat cttccagttt ttgcccaact
    12121 aggtggatac catccacagg aacttttgcc tctaccaatg cttgattgat ggctgctact
    12181 tggtcaattc cttcaaaaag ctggataaca cggctagatt ggacaaaata atccctataa
    12241 ccaagagagt cgagaaccgt tactgcttgt tccgtcgccg aagtgtcaat ttttataaat
    12301 tgacgagatt cttgtgccaa ttcttctttc gataattctt tgatcaagtg tccttcatgc
    12361 aaaataccgt agtgagtggc gatctgtgac aattcatcta gcaagtggct agaaagcaag
    12421 acggtgatgc cccgttcttt taccaaacgt tggatgattt ctcgcatttc tgccatccct
    12481 gctggatcca tcccattcgt cggttcatcc aaaatcaaaa attctggatt cgtgatcagt
    12541 gcatttgcaa tagctaaacg ctggcgcata cctaaagaga aattctttgc tttctttttc
    12601 cctgtatttt ccaatcgcat cattttcagc aggtcttcga tttctctatc cgacacaccg
    12661 ccgttcgctg cttggatacg taggttatct cttgcagtta attctggata aagagcaggg
    12721 gtttcaatcg attgacccat acgtcgtctt gctgcctgca aatccgtcgt tccaaataat
    12781 tcgatcgaac cttcatcaat cgagatcaaa cccataatag ttctcataaa ggtagactta
    12841 cccgctccat tctccccaat caaaccatag atcattccac gtttgatttc gatcgatact
    12901 ttatctaatg ctttggcagc accgtatttt ttggttatac cagttgcttt caagaccgtt
    12961 tcatttccaa aatccatact tatccttctt tcttattgat aagtgaagta tagttgacgg
    13021 gtataaagaa agcagccaga aatgtataaa gaaagtgtaa attttccaat tgaatttcaa
    13081 gaaaaatcag actgtctcgc agacgtggtt gagttgtctg ccaatcaaag agcgcaccaa
    13141 acagtgctaa tccaagtgga ctggccacca tggtaatcgt tgttatcgtt gacatcagac
    13201 gtccttgtat atgcgttgga acttctcgct gaagccagac catataaaaa gcaaaagaaa
    13261 acattaatcc ttaaaaacgg acttatgaaa aataatacat gccgtttggg atttacaatt
    13321 agctaccaaa taagaggtta tgacaaaaga cttttcacaa tcccttcttc cgaataaacg
    13381 gtgttctcct gccaactcct cggtcacaag tcacaatatt tatcaaacat gagaagcaat
    13441 ttttgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcagaagcgg
    13501 ataaataata ttgtttgatg atactttatc tttttcccgt ttgtttatag atcgaatgat
    13561 attatagacc aagaatccta cctattaaca tagctaaaaa cacaaaaaag gcactttcca
    13621 aattgtggaa agtgccagaa aagttaggat taaggtaact gcgctcagca ttgcttcgcc
    13681 cagtacgatt ctcgtaactc gtgaactcga acgataatcg caattaacgt tttgagaact
    13741 gtgaagcttt acgggctttc ttaagacctg gttaaacaaa cttttgtttt ataatgtttt
    13801 acagagaaag aaatcgtgta aaatcagcat ttaatgattc atataattct acttagttaa
    13861 ataaagttat atcctgtcat tacctcaaat cattaccaca acttggcgag ttggctgatt
    13921 gaagaatatt ctgtgccaaa taaaaaaaat gatgccaaaa ataagtacac tactcaatac
    13981 ttattttcaa catcattaat cctaaaatag cttcgctttt acccgttact atctaccctt
    14041 tcggaagggg tatttcatct gactaaagaa tagcatccaa gataccaata gtcaagtaat
    14101 taatttacca tactccatta caattttttc aaaaaagtgg aaaacttcta caagttcatg
    14161 attcttttcg tacattttac cattaacttt tatgcgatca agtaaatcag atagagcctc
    14221 aaagtttgtt gttttcaact tttgtgtttg aacatatttt tcgtgaagat atatagtagc
    14281 cacaatatca ttcattttca tattggtgag tgctctggcg attagttcag aatcaagacc
    14341 cgcattggtt gcaaactgtg ttaattgaat aacaggcctt cttttcttca agttgctgtc
    14401 agctctacca aattgataag gttcaacaat attataaata agaggtctac tatgtgcaga
    14461 ggcatttctg acattcgtaa catattttaa taatttttga gctgttttta atcctagata
    14521 tccaaaatgt tcttcttcaa aatagaaggt gataaatctc tctaaatttc ctagggtcat
    14581 aacttctaat aaagcccaaa ttgaaatctt atcatgatga tgattataaa aatcaaaatc
    14641 atatccgaac ttccctttca ctttatctag aatcttttca gaagcttgtg tatattcaga
    14701 caagatttct tcgtttgttt tagtattacc tttttttctt tgactatcaa taaatttatt
    14761 ttgcatatac atatcgtaat cttcaacaat tttatatcca tcttcggtta atgaattagt
    14821 gattgaactt attatcaaag ttttcaaaga atgttcaagt tcaaaaatca ttctactcaa
    14881 aatcattctt aaatacatat caatctttgc taaatcagct aattttacaa atgataaatc
    14941 tatatacttt ccatttatat ctttttcaaa attcttacgg tatgcggtta ccttaaaata
    15001 atacgttcga tataataaaa tatcctgaac aatactttca tccgattcag aaatttctat
    15061 tcctttaaat ctagcatatt taatttgatc cgtaattgaa aaaatttcct tgtttttagc
    15121 ttttttacaa agatctttaa tttcttctga taattcttca gagacttgat taactctctc
    15181 ttcttcttct aactgcaact ttaatttagc caatttgtcg cttttaataa aaacacacct
    15241 cctttatctc tttattttta tactatcttc ccaacatctc cataaacttc cgcaattctt
    15301 cttgcgcttc atctgttgtc ccgtgcaatt cacctagcat ttcaaataat ttatctgtcg
    15361 atttttctat ctcttgcttc acatctgcta attcttgtgc aaggtgtggt aatggagtca
    15421 cttcttcttc ctcaaacgtg tctacatatc ttggaatgtt taggttataa tcattctctt
    15481 taatttcgtc taaagttgct acatgtgcat atttttcaac atcttcacgt ttttcatagg
    15541 tttcaataat tttgtcaatg ttttctggtg ataatttatt ctgatttttc cctttgacaa
    15601 attcactaga agcatcaata aatagaatat cttttgttct cctagattct cttcctttga
    15661 ataccaacac agcagttgga atacttgtcc cataaaatag atttgccggc aatccgataa
    15721 cagtgtctaa caagttatta tcaatgatat ttttccggat tctcccttca gaagctcctc
    15781 ggaatagtac accatgaggc aatacaatcg ccatcgttcc agttttatct aagtgataca
    15841 ggccatgtag cacaaacgca tagtctgctt tagaagcggg tgctacgcca taacctttaa
    15901 accgtgtgtc tttttctcga tccacattct ttatatccca cttagcagaa tatggcggat
    15961 tagccactac agcatcaaat tttaatggaa tttgcatacc atctttttct gcgaatggcc
    16021 agtctgcatc aagggtatca ccacgtttca aattcatatt tcgataatta ataccatgca
    16081 tcatcagatt catacgagct aagttatagg ttgtcgtatt caactcttgc ccatagaaat
    16141 caacactgcc ttgcttattg ccatcgggta gttcattttt cactgtcagc agcaacgagc
    16201 ctgatcccat tgctggatca taaactcgaa attgagtatc atccgctttc gcatctttgg
    16261 ttacaatctt tgctagtact tggcttactt catgtggggt ataaaattct ccaccttttt
    16321 tccctgcatt ggcagcaaat tgaccaatta agtattcata cacatcaccc aggatatcgt
    16381 gaccagaatc atctttaaag gtaaaatcgt tcaccatcaa aacaacatca ttcaaggctt
    16441 ttgctcgttc attcgtattt gatcctaacc gagtatctcc aagattaatg tccgaaaaaa
    16501 cattggcaaa atcatcttcg gcaactggat tccgtttcgc attcaaattg aacgaatcaa
    16561 gcatatcctg aaagtcactc gctttgattt ggtggttttc aatctttgat actaactgtt
    16621 cccaagtata ttccggagca atcgcataac cgattcctcg actgatttct tccaagtaat
    16681 cttctttttc ttcagagtca gtgacttcgt agtactcgtc taacccattt ttttctaaat
    16741 actcgtcttg attttctgat aagtagcggt agaacataaa tggtaaaata taatttttat
    16801 actcacttgc atccattgtt ccacgtaatt tattggccat tgcccataat tttgctgtaa
    16861 tctcacttgc attactcatt aattttctct ccttttagac aaacattttt tgtagcaatg
    16921 tttttttcat ttcttttagt gaatccaact cacgctgatg aagggtgatg gtggtgtcga
    16981 gacttttaaa gaagtccgca attttttctt gctcgttatt attttttgga atcatgagtt
    17041 cagtttcaaa aaaatcagat gcagcaatat ttaaaagccc atgattccta gctccctcag
    17101 cagcatgctt agaaacctct ttataccaac gatctgtatc ataatatgac gctaaaaaat
    17161 ctgaatttat gtcgaccggt ctaaaacaaa tatacaatgt tgataataca cccatgtcat
    17221 acttatctag tcgcttaaca gcaccccaag gatagccatt ggagtagctt ttattatagg
    17281 caaactcacc tttcttcagc agataatatc cgctaacatc cttactggca atttgcttat
    17341 taaaaaaagt attctgatca actaatccaa attgtgctga tatagtcagt ggaagtgttg
    17401 attcaagctt tttgttcttt ctggtcaccc tagtggcaac ttcccccaac ttacgctgtt
    17461 cccaagcgtc agtaaatcca gcaaaacgaa tttctggttt atcttcacct gttttaggaa
    17521 acattttttt taaataagtt tgttttgttt gctctaacag atcaagctca cgctgatgaa
    17581 gggtgatggt cgtgtcaagg ctctcaaaaa aggtagaaat ttgcttttgt tcttcaatac
    17641 taggaaccat tactttaact gattttagcg ttggaaattt caaattccat gtatcagatg
    17701 ttaagccttg tgagtttctc ttgaacgttt gaatcatgtc atatcgcttg aaatcatacg
    17761 caaagaactt cgagtcaata tctttttttg gaatgataac tgtgtatgca gggcttagaa
    17821 tgccattata aggggaatat ccgcttgcac cttgccacat tctcattgag ttgtatgcaa
    17881 tatcaccaac ttcaactttc ttgtaatttg acttgtcatc actggaatta tcttttctat
    17941 caagttcgct tgcctttaca actcctgaat taatagtgac cgagataagc tctccttcgc
    18001 cactacgttc tgaccgttca gagaacacct ccccaacctt acgctcttcc caatctccct
    18061 cgtatccgtc aaatcgaatt tcaggttctt ttgttatttc attgctcatt ttaactctcc
    18121 ttcgatcttc gttatctcct tatcaaaatc agaaacgata tgctgatgtt tattgaactc
    18181 cttgtactca tcaaccgctt ttctcttagc tgctttactg ctaatttttc cgtttccatc
    18241 aagaatttga tagcgattaa atgttagaaa ttcatctatg cttttggcaa aatcttccat
    18301 acctaaaagt gtttcatctt ccaataatcg ctcaacataa tcaaaataag aagatacgtt
    18361 ccgttcaaga ctgcgaattt gtttttctga taaataattt ttagcaactg atacatctga
    18421 ctgtaggatt cttccatctg gagagttttt ccaagtcgtc agtcccatgt tctcttttgt
    18481 tttatctgca ccattaaaaa caatctccgc agctgttttt ccagtaatag catagtgaaa
    18541 tttattttgt acagtggcat aaaactgctt cgtaatttca gagtttttgt cataatcata
    18601 agagatttct gcaaatacat cagtaatttg ttgccaaatt cttcgttcac tggcacgaat
    18661 tgagcgaacc gtttctaata actctcggaa gtaatccttt ccaaaagcat tctcaccttg
    18721 tttcattcgc tctacgtcaa ttttgaagcc cttttgaata tagtctttca gaatatctgt
    18781 tgcccagatt cgaaattttg ttgctttttg agaattaact cggtaaccaa tagaaataat
    18841 cgcatcgaga ttataatatt tgaccttatt tgtttgtgtt tttccatcta ttgcgccatg
    18901 ttgagtggta ttttccaaaa tggaaacaac cacttcttca tctaattcac cagactcaaa
    18961 tatatttttt aaatgcttgc taattgctgg taccccaaca tcaaaaagtt gtgaaatcgt
    19021 cttttgcgtc gcccaaattg tctcttctcg aataagcact tcgactttat cactatcttc
    19081 ataaattaaa aactgaattt cattcaatag aacttactcc cctttcttag tctcgtccgc
    19141 catctgatag aaagctttcc gaaattcaat tcgatactta acccatgaaa gattcgccac
    19201 ttccgctgct gcgatttctt tataatctgt tcgtgcatcg ttcatcattg ctgttagttc
    19261 accttgtttg tccatatctt cttgaccaac tcgatgccgt gaaattaatt tggctaaatc
    19321 ttttggcttc acactattat ccaatcccca tgtgcgaaca aattctgtat atagtttgat
    19381 attgctatct ttttgagatt gttccatcgc ttgattcatt tcttcaacat ctctaggagc
    19441 cggatactta tcaaagatga attccttcgt aaagatcttg gaaacaaaat tgcgaacttt
    19501 cgtaatctcc ttatcgctat ccgattttgc aatttcaaca tcgatctcat tttttgtcag
    19561 ctcagcttga tccattttat cttcatgaac ctcatctgcc attcgtgcga tcaattcaat
    19621 cagataatca taattgatcg tcacatcgtg aatatgcgtc atctctaaat tgacttgcga
    19681 tatatcgata ttttcgattt gggctcggcg ttcacgcaac tcgttagcaa gtaccgttgt
    19741 cagcatgacc tcttgatcct ctgtaattcc taagcggtca tagaacagtt ccggttcatc
    19801 ataaacagaa acaggatttt tttcatcgtc ctccgtgaat tgcttcagtt gatttagtaa
    19861 tcgattgtac tcctgtaatc cttcaaatac ttcctcttgc gctttttcac ttggaggtaa
    19921 ttgaataaaa ttatctgtta gttcagctaa tttctgaatc acttctttgg tttctgattc
    19981 gattttacta aatgatttcg ataaaattcc cgactcttca ttgtcttttt ttaattcatc
    20041 tagcgatagt tgctctgctg ctgaatctcg atttgaataa atagcaaaag acttattcat
    20101 ttcgtattca ttttggatgg gccaacgata attaacaata tttccttgtg gctttgcatc
    20161 cctatcatgg attctattcg tacgtgaata tgcttgaatt agattgccac cttttaacgt
    20221 acgatcaatg tacaatgtat taagctccgg tgcatcaaaa cctgttaata gttgatccac
    20281 gacaatgact agatccaaat aatttccatc gtcagctgtt ttgttcaaac gtctagctaa
    20341 atcttctgta tatgatttaa ctgtcgtcat atcaaaagca gtaccaaaca atctattata
    20401 agattgaatt gctcggtgaa gattttcatt tgtttttagc tgatgactac tatttgatgt
    20461 atccatcgaa aaactaatgg caacttttat ctgatcctca attgaccgtt tattattttc
    20521 ttccataaat ttatcaaaat attccattgc tcgaggagta ctagcacgat ttccccctac
    20581 atgtactgta aataacgcat tgtatttccg attattagag cgttctttcc agttatcaaa
    20641 aatatcttca acaacgagtt ctacatgctc gggactatga tcatacacac tgccacgaag
    20701 attgtcgtca atatcttctt ctgtaggatc tgctggtgct tcaatcgtct ccttaaactc
    20761 cactttgaat cccaaaacgt ttttatcagc gatagcttct ttaatcgtat aagcatgtaa
    20821 taagtcacca aaaatttctc gcgtttctgg aaacttaggt gtacctgtat aaccaaccca
    20881 agcagagtta ggcaaggctt ttcgaataga ttctagcatc ccttcatttt tagtcccatc
    20941 acctgttgaa cgatgtgctt catcaacaat aaataataca ttctcagtta atggttgaaa
    21001 gctgtctctt gttacatatc gagacatttt ttgaatactt gttactatga tgtttttatc
    21061 actttttttg gtaagtttac ggtgcaagtc agaaatattg gcagtatcac ttacaacacc
    21121 agttttccct tcaaatccag cgattggatc atatgctcta taggcatctg ctgtctggtt
    21181 tgtcaatgca attcggtcta ccaagaaaat aaccttgtct acatttgata agcgactcgc
    21241 taaccatgcc gttttaaaac ttgtaatcgt ttttcctgaa cctgtcgtat gccaaatata
    21301 gcctagcttg ccctcgtcat gcttaaaatc aaatttccta actttatcaa gtacccgttt
    21361 tgttgcatag acttgatacg gacgcatgac tttgatactt tctttgttct ttgtaccatc
    21421 caacaccata tatcgagtag ataaatcatg ggccatggga atagacaata ctttatctgc
    21481 aaacagtttc catgaacgta ctggatgagc tgtttcttca tcttgccaat tgaaagcaaa
    21541 tgcacgattg aaattttgta atgtagtatt tgccatgtaa cgaatatcaa acggggtcat
    21601 cgcaatgagt atctgtaagg tagaaaaaat cccactaaat tgtttctcag caatatattg
    21661 ttccatttga tttaaagctt ctgtcgcatt atgtagcgcc tttttcaact caatttgaat
    21721 aatcggtaac ccattaatca aaagcgtaat atcaaaccga cgattgggct taccatcaac
    21781 aacttttgga cgttgaattt gattaacgac ttgatagact gtatttccgc cgccaacttg
    21841 ggcttgatca aaaacggtca aaaaaacatg ctttccgtta tcaagatcaa cttcaatttc
    21901 tgatacacca tttacaccat ataaaaattg tccagcttga taaggcgtat taatattggt
    21961 aataattttt ttcacttgat tgaactctgt tgcagataac ggttcatcta gccgagcttg
    22021 attattttgt tctagaattt ttttgaagtt gttccataat tgttctgttg ttttaatttc
    22081 agctttatat tcccattgtt ttacgccacc gattcttgtt agataatcca caacttcttg
    22141 ctcgaacctt aattcagatt gactttttat accgaattgc tttgtcataa tctattcctc
    22201 caaacttttt tctaacatag aatttgtaaa ttgctctaac aagcgagtat atatacttaa
    22261 cttactttgt aatttcaatg attcagtgta aatcccccct atttttcttt gttcttctat
    22321 aagaggtagc ggaataacaa gttgttccag agactgtttt gttaacctta aaactggacc
    22381 tgttccttga agacctcgat ctttttgacg ttgaatatct ttatgattgt taaataagta
    22441 aagaaaatag cgcttgtcta gcaaatcact tttaaactct acttttgtaa aatttagaga
    22501 cagaacttta ccaacattct gtccacttac cattgttgcc cgttgaagtg aattactaat
    22561 aacaacatcg cgtttatcta aagatacttc ttgatcaaat aaatagttta ctctttcctc
    22621 ttgagtcgct tcattataat acttatgatc ttcttcaaat gaagcttgat cgtaataatc
    22681 aatttcttga ttgccaaact gtttttcagc tcttgattga ttaattcctt gaacaaaggt
    22741 tacaaactca gaaagctttc tcatttccat atatacttcc tcctaaaata caattcaggt
    22801 ctcatttaaa cgatttatcg aaataatcat atatttcttt ttcgtttctg cgtttttata
    22861 ataaacctta tacagtaatt cgtcaatgga aataatttta tattacaaaa aatccgcaaa
    22921 aaatgcggat tcctttaaat atttaatctt tattctagtg tagtacaagc ctatacccta
    22981 acgttacata tctcattcat tattatacca gtatttcttc atgacacata agggtgtaac
    23041 ataataattt taaacgaaat gactacaagt agtcattgaa gtaagtcttc ataagatacc
    23101 tgtaggatat ccctaattgc tctcaattga tctaacttaa tgtgttgccg gccaccttca
    23161 attttaacca atgtttccct agttatagca attttatgaa gctgtaggtc tctaatcagc
    23221 tcggtttgac caattccttt ttctaatcgg attcgtctaa tattgctacc gattacattt
    23281 tctttatcaa taattttttc ctctttcaag aatagcacac cttttggact aaaattagtc
    23341 ctttttctta ttaatcttaa agattcggta tgctacaatg ggactagtat tagtccacat
    23401 tcaattttaa ggaggaattt ttttgaagaa atttgcaatc attctaacct gcgctttact
    23461 catcggaact ataagcgggt gtaccacttc tgaaacaagt tctacaagta cggaaactag
    23521 tacaacaacc tcagaaaaga attacccaat acattttttt ccggttccag atggatttga
    23581 agcttattat catgataaaa aactagatgt ccctttatca gaaactgaat ctactgaaaa
    23641 gattttgcca attgaaattg aaagaaatca agacgatata aaactaaaag ctgatcttcc
    23701 cttactgaac agcacacaaa ttgatagaca tgaaaatatt aaagttaata ttgaaaaagg
    23761 agcgttagca aaactttata ctgaaaaagt tgaacaattt tttaagtctg gtgccaataa
    23821 agacttttca aaaataagcg attattcgga gaactttttt aatgaaaccc aagatgaaat
    23881 catgaactca acttttatgg ctgaagatat gtacaatatg acattaaaaa aaataaaagc
    23941 ttacgaccat ccgttaaatc tgacactaaa taatggactg ctttcttttg tattagcagg
    24001 ttacgttgac tatgaatatt caccagatta tttggagaaa cctcttcaac acgagaacaa
    24061 tgatattaat tttagatttg tctacgagtc aaaaagtaaa acatggttag ttgatcaatt
    24121 aagcacgata aacacaagct tccatgaatt acctaaacct gaaaatgaag ttgagatggt
    24181 cattaaaatc ttctaaaaaa gaagcgttga aggagaaata ttcctttcaa cgctcttttt
    24241 tatatgctta gaaacataaa taaattgtat actttctttt tagcccctat tacttttcac
    24301 gaaaaatatc tttttttcga cgctagcgtt tctctacttc taatactgta cacagttttt
    24361 gtattattga aaaattactc attatttagc attaatccac atcgttttat caatcgacct
    24421 tcatactaaa ctactttaaa gtcatcttcc ttctcgtttt tcattaaatc agatacataa
    24481 gcccatgtac ccgttcgttg taaataaagt tcttcttgta acaactccca ttttctctta
    24541 gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat ttctatttca
    24601 gaatttgaat gaactacttc ttgcaacact tggtgttagt atcaaatctt agacagcttt
    24661 tcgtagagac aagaataaat taaacttatc tacgagagga tgaattttta tgacccgata
    24721 tgaagaaaat tttaaacaaa tgattgttga actgaatcaa actggacgtt ctgttcgagg
    24781 gttagcgaaa gaatatggct tatctgaagc aacgatttac aaatggaaga atttatattt
    24841 acctgatcag tccacaggac tgactggaaa agaagtagct gaactgagaa aagaaaatgc
    24901 tcgtttaaat gaggaacttg aaatcttaaa aaaagccgca gccatattct ctcggaaaac
    24961 ctaaattcgc ttgttcaatt tattgaaaaa tggtgtaagg attacactgt ttctttgttg
    25021 tgtcggttat tagaaatccc tagaagtgtc tattattttt ataagaataa accgttgaca
    25081 gctacagaaa tcagaaataa tacgttgaaa aagaagattt caacaatttt ttttacgaat
    25141 aaacaacgct atggtgccac aaagatccat caagttttat taaaagaagg gatttcagta
    25201 tctcttaaac atgtccaaaa gctaatgaaa caattaaatt taaggtcgat tgtagttaaa
    25261 aaatatagac ctcaaaggtc taataaaccg atcatttcaa aagagaatct cttaaatcag
    25321 gacttttcta ctgaaacgat ctgtgagaaa tgggcagctg acattacgta cattcctact
    25381 aagaaaaatg gttggtgtta cttatcttca atcatggatt tacacacgaa aaaaattatt
    25441 agttatacat tttcaaaacg aatgactgtg gattgtgtca ttcaaacatt gaataaagca
    25501 aaaatacatt atcacattcc agaaggaatg attctacaca ctgacttggg cagccaatac
    25561 acagcaagag aagtggaaca atggcttaaa accaacaaaa taaggcattc ttatagtaga
    25621 aaaggaacac cttatgataa tgctggaatc gaatctttcc atgcctcatt gaaaaaagaa
    25681 gaagtctaca cgactagcta ctcagatttt gaagaagcaa atcgagcgct atttagctac
    25741 attgaaggat tttataaccg aaatcgaatc catagctcga ttcactatct aaccccacag
    25801 gaatttgaag aattagcaaa agaaaaaatg gcttaaccgt ctctactaaa atgtgtccaa
    25861 gatattgact caaatccaac tttacctata ttttctttga agtttgttgg agtaatagta
    25921 tccaaaatga acacctctct tctctatacc tctattatga agcgcccatc aaataaagca
    25981 agcgttgagg aataattcta tcaacacttg ctttattaag ctttcatttt gtagtttgac
    26041 taaaaccaat aatcttattt ctaaactctc gaaaaacttc ttgtggctga taatagtctg
    26101 catctatttc aaatgtttct gagaataatt cattgaattt ttctactgaa ataatgattt
    26161 taccattctt ctctcgactg ttttctatta tttctgcttc taattcaaat aaatctagtg
    26221 acgaattaaa atcataaatc ctgtcatatt gaacaaaaaa atcaagaaaa agttgctcaa
    26281 actctttctt atttatcact agtgtttcaa gcagatcaac tttattttct tcagtcattc
    26341 ttccaatttc ctcactttcg ttttcaaaaa tcacaaaaat gatattaact taaaagccac
    26401 taacaaaata agacttacaa attaaagggt aatccaacaa aataaccatt cgctctacgt
    26461 actacgattt ggtagtcctg cctttcaatc ggtaattcat aatagtaatt tactgtttct
    26521 gatttaccac tttcaatata ctcaggatta tccaatatca tagcatactc ataaagctct
    26581 tcaccatcct tattttcaat cgaccattgt tgcggacgta tcggattttc tgtatcattt
    26641 ttcattgtat aggtcacttg taatacacga tcatgctgtg caaattcatt tcttttttcg
    26701 gtcagttcaa tatttttaaa tgttagttca accccatctg cctgataagt ttcacctatt
    26761 tttaaatctt ttgttacaat ttttattggc ttttcttctg gaaaatacct ggctttaatt
    26821 gttagataac ttccaaaaag aatcaatcct agtagcacaa aaaaaattcc tttaacgagc
    26881 cactgaaacg ggaagtaaac tttaaacatc ttttttctcc tccgttttgt atttggtttt
    26941 tagagaacac gataactttt gatcaatacc atccaacttt ttcattatac ttgattctag
    27001 ttctgaaatt gtttgttcaa gtttaatgct ttcaggatca ctaaattttt tatttgattg
    27061 actagccaac attggtaaat ttgaaaccat cacacggaac atatttgctt tcgttccaag
    27121 agttccttta ttcttctcat aaaagctgtc aaacaatttt tttgtttcct ttgaacaata
    27181 tactttaata tacggatcag acattctttt ccctctccct gtttacttaa aaatccatat
    27241 catattgatt ttgttgtttt tgataatcaa ttcctcttgc tgtttcttca tactctctcc
    27301 gagctttttc aatcgtacca tcatcaattg cacgattaaa taaataaaga acacgttttg
    27361 tttcttgaat ataacgcttc ttaaaattac gttttaaagc gggttcagta tcttgaatgt
    27421 taaattggtc ataatcagtt gataaactct ctgcgatatc ataaacaact tttttataca
    27481 ccaaatttac acatttcttc ttatatttta aaggttcctc ttccactgca aatccaaaga
    27541 attggttttt attctcacca aaaacatcca ataaattttc tattctgtcc tctatttcaa
    27601 atttatattt atcataaaat tcttcataat cttttgatga tctaagaaat ggtatattct
    27661 cattatctaa ttcaaagcgt tgcgctcgtt cttgccaatt aaatctacta tccgctctac
    27721 catttaacga tttaccaaga tatcgaatag caatttcagt gagtacctct tcagattgaa
    27781 tttgctccgc ttttttattt tctgtgaaca aatcatcaag ataattcata tcatagtttt
    27841 gccctaaacg ctttccgcca atctttaaga ttaacccatc ttcagaatct aagagtttgt
    27901 acccaatacg ctgatttttg gcaaaccggt aagtctcaat agaatggttt ttcttcaatt
    27961 ccgcatgaaa atcatcataa tttttcgcat actgagcaac ttcagaaaca gctagtttta
    28021 ttttttctcg atttgaaatc ttattttcag ttctaaattc acgttctttc caattttcac
    28081 gatctgcaac taaatgatta tacttataca tatcaagtgg gttaattcca ttatcaataa
    28141 gtatattatc attccaagcc tgcaaaactc gaatatcatt ctttcgaata tccattttct
    28201 ttcctgttcg ctgatcgttt ggcgaaatac caaagtgatt gtgaacatgc cctttgtctg
    28261 tgtgtgtgac aaccaaaatt gactggttag gaaacatttt ttctgcccac ttaacaccca
    28321 actcatgtgc tttttcagcg gaaatatctt ctagtggatc aaagctttgt gtaaaatgcc
    28381 aaagctttat tttcccttta tcaccaaaaa tttcattggt tcgtttaaat tcctcaatag
    28441 catttaaatc attcgatacg ccaattcctg tcacgagttc agaatcttct tgtttattta
    28501 acatatatga aattgtatta tgaatactcc tactttttga agcaattttt ataatcgagc
    28561 ccataactta ttcaactctt tttccaagcg attcaatgga tcacttattg attttatttg
    28621 tatcattaag tcattttttt cagcatatag ccgctttaat cgctcttgct tagttgtaaa
    28681 agataagtaa acactctgat gttcttcttt ttgtatctcg gtatcttttt tatttaactg
    28741 aacacgcaaa taatttgctg ttttcactaa ttgattcaaa tttcttccta ttgctagtaa
    28801 atgaaaatta atttctttat attgtcgatc ggtatatttt agacttttcc ctgcgttgtc
    28861 tactgcgatt ttcttcacgt acattggaac agataaacca aaaatgtctg ctgttttttc
    28921 tagctcttgg tactgataat cgtctactcg aaactttatc ggcctatttt gtcgtcgact
    28981 atcttcatta cgtttatatc gctttctcct ttcttctaat ggtagtttac tgacatcaat
    29041 tgccatgcgc acttctcctt tgcaatatta tttttaagtt caaaagaagc aagcgagggg
    29101 aaagggaacc cttccccgct tgtcgaggga gtaaactccc tacgagcacc ctcatgctcc
    29161 gcattttatc gagattagat agccgataaa tcagctacct aacactttct tcttgcgcct
    29221 cttctcgata gaaaattcga tacgctgtct ctccttttct tctcaaaaat tgttggaatt
    29281 tcgtttccat caattgatcg cttgcaagaa attcgtgtac tagggtaaat tcgctattcg
    29341 cgaaatttac cgccttttgc catttttctt gagtaccttt ttgattaatt tcaaaagttt
    29401 cttcaaactc ttttactgat gaaattcctg ttttttcata gacatacttt cccagttcgg
    29461 gataaagttt tctctccaac tcggcgagtt cttttttcaa atctgcaatt gacttttgtt
    29521 ttttccttgc catttttcat gctccttttt gtatgtgact aagttcattc ataagctcat
    29581 ttttcatcac taattcttct tgaatcatca gttcaatata atttgtgacc gtgatttttt
    29641 catttgtcgc tttctttact gagtcgcata attcggcaag ttctaagaaa tcctgcgtac
    29701 taatctgtag aattaattgc atgacaatca cctctttatt ttttaaatag actatcaaac
    29761 agcggatttg gtttatcaga aatatcctga aacagtggat tattttcata agtaaatcgt
    29821 tgacgtttct tctccttcac ttcttgttct actatcactt tttcattagg tctttctttt
    29881 cgttcctcga caatatttat tttttgcgtc ttatctccct gacttgcttc agctcttgtt
    29941 tcacgtactt ctaccgttct tggttgattt gacgcactac cttgtacatt ttctcgtaca
    30001 tctactgttc gatttccgct ttgactactt gtagtattct gcaagttttc tcgtacatct
    30061 atcgttctcg gttgatttga cgcactactt tgtacatttt ctcgtacatc tactgttcga
    30121 tttccatttt gactacttgt agtattctgc aagttttctc gaatatctac cgttcttggt
    30181 tgacttgtcc cactactttg tacattttct cgtacatcta ctgttcgatt tccattttga
    30241 ctacttgtag tattttgcaa gttttctcga acatctaccg ttctcggttg gcttgtccca
    30301 ctactttgta cattttctcg tacatctact gttcgatttc cattttgact acttgtagta
    30361 ttttgtaagt tttctcgaac atctactgag cgcctatctt gactacttgt agaattttgt
    30421 ggattttctt tgactgattt ttctaatccg gtatttgaat ttttagcttc ttggattctt
    30481 tctttagtca acgcttcttt ttcgtccata ttagcgcctg tacgcccttc tacacgtttc
    30541 cttaacattt cctcacgatt cattgcttca ggcgtgtatt ttgcttcctg tcgcttctga
    30601 ttaattaatt cttcagcaga cttagcttga ccgtgcatag catttttacg ttcttcaata
    30661 tcttgtttaa ctttttgctg aattgcactt gcaggattaa tatttgattc agcattattt
    30721 ttcattgctt cacgcatacg ttcagaacta gacataattc tatcagcgtt ttcaacttta
    30781 gcgccatctt ttctatccga aagacgtgtt ggcatagaat cagcatattt tccagtttca
    30841 tcttctttat ttttagctac tataggtttg ctatttttat taatagcgga catggtacca
    30901 tcatctaaac cttctttgaa tttattcttg gtatcattag ccatatcacg catactctta
    30961 aatggttttg ttgctctttc actagccatt gtagctccat ccgaagctaa gcctgataat
    31021 ctacgttcac gagcataccc aagcgttttc cccgttttat tagctttttg agaaagtgat
    31081 tgaccaaagt tgcgttcagg ttttcgttct tctccttccg cttgatcgtt tccaccacta
    31141 tttttcccac ctttagatcg gttcatacca cctgttcctt tacgaaacag cattgcaccc
    31201 atgccaaaag cagaagcaag ttgcccatag ccttctttga gtccaatatc cacaccaaag
    31261 taacgcatga ctgttccact gcctttgatt aaaacaaaca cagcacagat ataagcaatt
    31321 gtttttataa agatatttgt tgagatacct tcaccagcat ttagataatt gacaaacatt
    31381 gcaaacatag acaatccgaa cccttgaaat ccgactgtca aataacactg taaaatatct
    31441 gacaaaactt gttttgatcg ttgacctgtc tctaagtcag tagcaaatac caaaactcca
    31501 agtacacgtt taaatgctaa ctcgagtatt gctgtaatta taatgaaagc actaaaaaca
    31561 tacgctactg ctaaagcaat caatccaaat gaaattcccc acaaattcgc ttgatagcga
    31621 taatatcctg atttgatacc atcaacaaag gttgacaaaa aattatctga aaatttagta
    31681 gcaacaaatt cattatcaga attttcaact aattcataac gcaggttatc agctgttggg
    31741 ttctcttttt ccattttatc aattacatta tctgtcaata cttgcgtaag atccgttttc
    31801 caaaaatttt cttttgttag attattccgg tcattcgttt tgttaatcat tgaatactga
    31861 tctgttttat tgatataagc taaatcagtg accccttgtt caatcagcga ccacgccacg
    31921 ccttcgtccg aatttgtaat cgtagtcgta tcattataaa atgttttaga aaactctatt
    31981 ccactgtgaa tcaaagtcgg cattaaaaga atcaatgcta ctgacattac aacattcatt
    32041 ccaactgatt ttagatcaac cgttccttta cctgtaatca ttttataacc aataatcact
    32101 agtgaaagta tcattaagcc aacaacaatg gtacttaaaa ctgattgata gactttattc
    32161 aatccggtac tttctacaaa gctaaacaaa tctaaaacct cgggaatcat attttgaata
    32221 ccatcgacaa tagcgaacat tgattttata acagaccagc ctatccacat accgaactgg
    32281 ctgtaaaaat ttccgttata ttttttaaga taattctgat aaagtggatt agttaaaaac
    32341 tcagtttgac tctcagtatc atcaaaccag ttgaaaatac tgccttctgc aacgttactc
    32401 gtaaaaataa tcatagctat gagaataaga aaactaatca acaaccaatt tatttttcta
    32461 ttatcaatct tttttttaat tattttcatt gttacctccc tcagcccatt tttttaggct
    32521 ctcagaaagc ttctgagcca tttttttatt aacgatgatg tgagttacgt aatcatcatt
    32581 aacagctctt aaatctagaa caatagtatt tgagcagata ctctcgatta actcaagagt
    32641 atctgcgtta tgatcaagac agtaaatctt atctactaga tgattttcaa tcaaaataac
    32701 ccctcccgta gtcaaagttc tcaattttat gaagatatcc ctctacttca tttaaatcaa
    32761 ttctattctt attgctgact aacccgttgt aaaattcttt ttgtagatta tatttatctg
    32821 tttcttgaat ctttttatat aaatcaacca gggagtccct gtctttaaaa acttgcttga
    32881 attgttcgct tgtatatcgg ttttcattaa ttgcaacaac tgcttgaatt tcaggccatt
    32941 tttctttcaa cttaaaataa cggggaacgc tgaaatagtt ctcaaaaaag aagtctcgtg
    33001 cttcatagta ttctctattt tgtcttttta tatcctcgac aatttcgtat cgtgtagaga
    33061 tcttcaaagc tgaactttcg ttaatttctg ttccttttcg aaagactttc acttttgaag
    33121 ggcgttcaat tttataattt atcccctttt tacttgaata acttttgata cgaccatcaa
    33181 attgaaccat atctcctttt tttaaccacc caaattttag aaattgttta gttaaattca
    33241 tccaaatgtg gtctgtgacc aactctaagt tatccccttg aacaattttt aaattcgata
    33301 acataaaagt agcattttca tactcttgac caaacttgta tttatccttt ttatatccgt
    33361 taaattcaaa ggtaccttga aatgtatgcc gtttcttatc tcctaatttt ttcaattttt
    33421 ctctcaaaat aatcaactcc atcatttaat atatttttgt gatggtagat actctcgatt
    33481 aacacaagag tatctgcgtt gtaatcaaga caataaattt gatatattaa ccgtttttca
    33541 atcatattat taatcctttc taacctattt ttttcagtgt ctctagcata tccagtctac
    33601 tagctttttc ttgcttagct atgtcctccc agtggttcct agctgtggtg ttgataaact
    33661 tcatttcatc aatttcttca ccgagattca gtttttcatt tatagaatta aaaagtcttg
    33721 ttagcatttt gtttgttata tcaaaatagt cttgacctcg aatttctatt ttatattctc
    33781 tttcaattga atctttcata ctagcaatca acttaagata cttttctctt tgaacttcag
    33841 tatatctatc acctagtctg gtaattattt gaattccttt ctgatttata atattttcaa
    33901 aaaaggaatt ctgatcttga ttaatctctt ctatactatc cttatcttga ttagtttgat
    33961 cttgactatt ctgatcttga cttaactgtg tatccgattt gacgtcgcat ggtataccag
    34021 ctggatacca tttttccatc agatcaactc ttaatggttc tacttgacca gactgcaaaa
    34081 tcatatcaaa ttttattggc tttagctttg agaaaaattc tggttttaag ttcaataact
    34141 tgaatcgata aatagaaggt ttatacctat cgattcgtat attttgattt tgtaaccagt
    34201 ggggtagaaa cgctaacaaa tcttcattta aaaaaaatac aaaacctttt gtaaaaagta
    34261 tctttacatc atcatcgggt gttccgagca ttttcgtgta tggataagct tcgacaattc
    34321 cgtcatcatc tgcgtttgtg ttcagatata cgtataataa ttttgctgaa tctgacatct
    34381 tcataaaact tgcattattt aaaaatgatt tagccagcat tcgtttttct gccataaaaa
    34441 tcacacttct tcctatttga atgttaaaat gtagtcaaat aaagtttagc tagaacttta
    34501 ttattgagcg ttcctagtgg tctgcaaact aacgggaaca ctctttttta ttcttattaa
    34561 aaccagcaat actagttcac cttctttcat ccttttacat ccaattaaat ttgatgctgt
    34621 agcatccatt tttttacctc ttcttttgaa tatagttcaa ttgtgtcaac cacgataata
    34681 ggtaatccta accttttaaa cttcagaaaa gttccatatg atacgtttag aaattttgcc
    34741 atttcttttc ccttaagaaa aggtcgttct aatccgctgt ctattcttgc ttgatcaata
    34801 gcttcttttg tcacttcaaa cacatgtgtc ttcaactgat ttgtttgatc agtattcaac
    34861 attacactga ctccgatttg ctccattaaa aattcccact ttcatatttt catcaataag
    34921 ccaactcgcc aagttgtttg ttaataatct gatttcaata accaacctat tattttttga
    34981 taaacatttt tcgacacact ttgaacttcg aagcattcaa tttttttaag agtttgcctt
    35041 gaaattccaa ttaattctgc tgctttagtt ttcgtaatca tcaactttcc tcgtctctct
    35101 cgaattctta tactaaaatc ctcattaatt tgaaacataa acatccctcc atttttattt
    35161 ttgcatttat ccgtttatcc gatgtcggat aaatagattt tagtatatat tattttgata
    35221 gtcaattaaa aatatacatt ttggataaaa ttgttcattt ttttatccaa atcatatatt
    35281 atagatagta agaggtgaac ttcattgact caaaaaaaca gaattaaaga aactcgcgaa
    35341 gaacagaatt taacacttaa agaacttagc aatctgtcgg atattccgta tcaaactttg
    35401 agaaactatg agattggtaa aagaaaaccc agaaatgatg aagtttggtg gaagttaggt
    35461 acgattttaa acgtccgtgg gccctactta aaaggagaag atagttttaa aacaaatgat
    35521 gaattgcata tttattatct aaaaaaaagt catacaaatt ttgaactgtc taagaaagaa
    35581 gaaattatag atataataga taaactaaac gaagacggtt tagatgaagc cttacggcag
    35641 ttagcactat tatcaaaaat agatgatttc aagcggtaac ttacttatca tcatgtagac
    35701 tatcaggtat cataaactaa ttcctcaatc agccaactcg ccaagttatc agattggaga
    35761 gattagttat gtcaatacaa aagtacaaaa caaaaagcgg tacacgatat agtgtccgct
    35821 tatatgttcg taaagatgaa aatgggaagc aagaatacta tgtaaaaaaa ggcttcaaaa
    35881 ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa gatatgggct
    35941 atcacagacc ttctaatgac ttatttagaa cagtctatga agaatggttg ccgtcttata
    36001 aaaatcgtgt acaagaaacg acttatcaac gaacaaaaga cctttttcgc ttgcatatcc
    36061 tgccaaagtt cggagacaaa tcgatttcta agattactcc tatttactgt cagaatgcaa
    36121 tcaatgaatg ggctgtaaaa tatacaaata tcaagcaact taaatcctac agctctcaaa
    36181 tatttgagta tgcaatcttc gcagaattgt tacagcgtaa ccctatgaca aataccaaac
    36241 tgccaaaaca agaaaagaaa gaatccgaaa attatttctc actagaagaa ttaaaagagt
    36301 ttttggaaat tctcaataaa gaagagtctt taaaacatat cctgatcttt caacttttaa
    36361 ttactaccgg tgttcgcaaa ggtgaattat cagccctacg ctgggcagat attaattttg
    36421 aagaacagtt actacacatc ggaaaaagtt atgcaacaat tcgcactgaa gatccgaatg
    36481 ctactagaaa aacaatcagg attcagaaga atacaaaaaa tgtctcttcc gaacgaattt
    36541 taccgattga tactcaaacc atcgaaatgc tgaaactttg gaaaaaagag caagcagagg
    36601 tacttttaca attcggcatt aatgcaaata ccaaaaagca acttatcttt acctatgtaa
    36661 atgcaaatgg cgatgttaac caacctttac acgcagatta ttccaataat attatgagac
    36721 gtttagagaa gaaatataaa tttaagcacg ttactattca tggattgcga cacactcacg
    36781 ccactttact tttagaaggc ggtgcgagca ttaaagaagc acaagatcga ttgggtcata
    36841 aaaatgctga aactacgctc aatacttatt cccatgtaac tgaaaaagcg caacgaaatg
    36901 cggtagacaa gtttattgct tacactggat tatagacaaa aaagttgccg tcattacctt
    36961 tttcattacc acaacgaaaa aaagcacctt ccaactgaat ggaaagtgct tgaaatcgtt
    37021 ggtataacaa cgattaacgc ttagagaatt gtgaagcttt acgagctttc ttaagacctg
    37081 gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta
    37141 aacggaagtc aggatcaact tctagcaatg cacgagcgat accatgacgg atagctcctg
    37201 attgtccagc gtatcctccg ccgtttacgt taacaaatac gtcatatgcg ccttttgttt
    37261 cagtaacagc gaaaggttga ttgattactt cacgcaagtc agcatgtgga atgtattctt
    37321 caacatcttt tttatttaca gtaattttgc cagttcctgg tactagacgt acgcgagcaa
    37381 ctgcgttttt acgacggcct gtgccgatat attgaacttg tgccaatgaa attccctcct
    37441 attagattaa gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt ggatgttctg
    37501 ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca
    37561 taccttttac agaagtttcg atcaaacgac gagaattttt agcacgcaat tcgccagcag
    37621 tgattgattt caatcctcct gggtagttgc tgtgacggta ataaattttg tcagtcgctt
    37681 ttttacctgt taatttcact ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca
    37741 aatgaggtgt gaaagttggt ttatttttac cacgtagtac agatgcaaca actgttgaaa
    37801 gacgtcccaa tggaacatca gttgcgtcta ctacatacca tttacgttct acttcgcctg
    37861 gtttggccat atatgttgta cgcacgttat tttcctccaa tttaagttct gatgtttgac
    37921 atacacttcg agtttccggg gctctttgtg gggcaaacaa taccatttaa catattaccc
    37981 gctaatctag tcaatgtcaa cgtttttctg cttgattgaa aaatttatat gaaactttac
    38041 ttcttttctt tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg
    38101 ctgtgacaaa agtcttgtca caacccctta ttttgaataa acggtgttct cctgccaact
    38161 cctcggtcac aagtcacaat atccataaaa cataagaagc tgttttttaa aattgtttct
    38221 tgtgactcgg agctgaacgg caggcttaca acctcttggt aaacggtgtt caatcagctg
    38281 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct
    38341 ctaatactca ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag
    38401 tttttacgcg tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt
    38461 ttctgtggct gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc
    38521 agaaacttcg attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt
    38581 ttccaaacgt tctggaatag tacgatccca gcggccaatt tcatcaggga tttttgtgtt
    38641 atctgcaaat tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga
    38701 tacttcatct aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt
    38761 aaggaaacct ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct
    38821 tagatctgca actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc
    38881 ctcctaacat tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa
    38941 tcatttacct gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca
    39001 gaatcagcta attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt
    39061 tgtgtcgaaa caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact
    39121 ccaacttgct ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg
    39181 atgcttctcc agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa
    39241 acaagcgggt aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac
    39301 ccgcctgtag cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga
    39361 taaaggttga gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc
    39421 cgctgtaaac cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca
    39481 agtaaatagg gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa
    39541 agcagtgcat catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa
    39601 aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc
    39661 caatgtttct gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga
    39721 gtagtaataa aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca
    39781 aaagcaccta accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata
    39841 tcatttggca aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg
    39901 gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct
    39961 gcccattgga attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg
    40021 atcagttcgc caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt
    40081 tttttgaata attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt
    40141 ggatgaatcg gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt
    40201 ttttctgctt cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt
    40261 ttctcttcat ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg
    40321 attgtgtctg aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag
    40381 atcaattcct gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa
    40441 taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt
    40501 ccgatatcta atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa
    40561 aatttagttt aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat
    40621 tttatcattg gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta
    40681 ggtcactcta ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg
    40741 tatgaattga ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg
    40801 tgtcggttgc ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg
    40861 ttctttactt tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg
    40921 ctattttcgt tgatggattt attttacttc atggtccatt ctcacacgct attctgttct
    40981 tgggcaattt tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg
    41041 ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc
    41101 gatactctgt tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc
    41161 ttgtttcttg ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa
    41221 aaaaagttgg aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt
    41281 catcttaggt attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt
    41341 gttctcttac caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta
    41401 aatggtgttc aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg
    41461 atatacggtg ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga
    41521 cgaaggaaac ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc
    41581 ttgtaataat cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt
    41641 gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca
    41701 atatttcgtt cttcttcatc attatagtag atcactggac gataagaatc accgcgatca
    41761 gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga
    41821 tcataagaaa taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg
    41881 cctgttgtta cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc
    41941 acagaaatga tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct
    42001 gcgaaaactg ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc
    42061 caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata
    42121 cggcttctga taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca
    42181 catattcagc agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag
    42241 ccaaagctgc ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta
    42301 cattcaattg ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt
    42361 gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc
    42421 gtgcattttt gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa
    42481 gaacagctat tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat
    42541 atagattgtt tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca
    42601 tttttccaaa tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct
    42661 cgttttcttt ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt
    42721 aatgataacg agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt
    42781 tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt
    42841 gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag
    42901 gatcggaaaa acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact
    42961 tccgcaaata gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc
    43021 ttttcctttt agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg
    43081 ctgtacccgt ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt
    43141 ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc
    43201 ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc
    43261 taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc
    43321 gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg
    43381 cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt
    43441 ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc
    43501 gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg
    43561 tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg
    43621 gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc
    43681 gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa
    43741 ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag
    43801 atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc
    43861 taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc
    43921 ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt
    43981 aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac
    44041 ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga
    44101 gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc
    44161 catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct
    44221 aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg
    44281 caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca
    44341 tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac
    44401 agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa
    44461 tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc
    44521 ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat
    44581 aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta
    44641 acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat
    44701 tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca
    44761 gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa
    44821 aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg
    44881 atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat
    44941 gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca
    45001 ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac
    45061 agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt
    45121 tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca
    45181 ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc
    45241 gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct
    45301 gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg
    45361 gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc
    45421 tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga
    45481 ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta
    45541 ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt
    45601 tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct
    45661 acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta
    45721 ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg
    45781 agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact
    45841 ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct
    45901 gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca
    45961 aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat
    46021 ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt
    46081 tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca
    46141 ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga
    46201 gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct
    46261 tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag
    46321 cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa
    46381 acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat
    46441 cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac
    46501 caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca
    46561 tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt
    46621 tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt
    46681 aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac
    46741 gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc
    46801 ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga
    46861 gggcagccaa tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg
    46921 gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc
    46981 ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat
    47041 ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga
    47101 ttgctacttt cttcatttat ttctctctcc tttgcttatt tacttattgt tattctttct
    47161 ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg
    47221 caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat
    47281 ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc
    47341 agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat actctttttg
    47401 cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat
    47461 gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt
    47521 gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc
    47581 ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct
    47641 tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac
    47701 gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc
    47761 attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt
    47821 cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa
    47881 caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc
    47941 taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct
    48001 cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa
    48061 gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa
    48121 ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa
    48181 tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct
    48241 tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc
    48301 ccgccatagc cgatccctat taaagaagct tgtaatgcgg cagcactgcc actcgtttca
    48361 ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct
    48421 ttcataggat ctagcgtgta atcggcaccg tgtttttctg ctaattctct acgctgcgct
    48481 atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt
    48541 tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc
    48601 cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc
    48661 aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga
    48721 atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt
    48781 tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt
    48841 tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata
    48901 aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt
    48961 actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct
    49021 attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta
    49081 tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat
    49141 aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc
    49201 tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag
    49261 gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga
    49321 aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag
    49381 accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag
    49441 catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt
    49501 atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga
    49561 aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt
    49621 tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga
    49681 gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc
    49741 ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc
    49801 ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa
    49861 tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag
    49921 tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa tagaatcgat
    49981 atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct
    50041 aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata
    50101 gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg
    50161 aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact
    50221 gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac
    50281 aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa
    50341 agctgcaacc tatctactaa cagatcattg acgattccat aactattatt gaacatatat
    50401 ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc
    50461 accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct
    50521 gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat
    50581 tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta
    50641 ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact
    50701 aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa
    50761 tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt
    50821 ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa
    50881 gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct
    50941 gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt
    51001 acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca
    51061 ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga
    51121 gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca
    51181 gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca
    51241 tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct
    51301 tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg
    51361 aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt
    51421 tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca
    51481 tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt
    51541 ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga
    51601 gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt
    51661 tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt
    51721 ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc
    51781 tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca
    51841 aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt
    51901 tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct
    51961 ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag
    52021 tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca
    52081 gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc
    52141 cacctcttat tttattattt tcagccacat atattcatac ggctggaata tcaactttcg
    52201 ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa
    52261 aagataacct gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc
    52321 ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg
    52381 atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc
    52441 tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg
    52501 attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag
    52561 cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc
    52621 taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata
    52681 cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg
    52741 ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc
    52801 tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt
    52861 tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg
    52921 aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc
    52981 gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac
    53041 cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc
    53101 tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg
    53161 aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac
    53221 ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt
    53281 ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac
    53341 gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa
    53401 gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa
    53461 cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt
    53521 gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa
    53581 gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg
    53641 caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc
    53701 ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg
    53761 ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag
    53821 ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat
    53881 tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata
    53941 caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat
    54001 gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac
    54061 tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat
    54121 ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa
    54181 ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa
    54241 ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc
    54301 attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca
    54361 atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac
    54421 accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat
    54481 tattatttta agctctacga tggatgaaac acttcttttt gaattggatc aatataatat
    54541 tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac
    54601 ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca
    54661 tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta
    54721 ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg
    54781 ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc
    54841 aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca
    54901 atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc
    54961 ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa
    55021 agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat
    55081 tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat
    55141 tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt caatcactca
    55201 tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga
    55261 tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact
    55321 atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt
    55381 atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat
    55441 gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa
    55501 taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt
    55561 tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat
    55621 gttgttaaga tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa
    55681 aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat
    55741 tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt
    55801 cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag
    55861 ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac
    55921 cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga
    55981 aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag
    56041 gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata
    56101 atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt
    56161 gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac
    56221 gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt
    56281 gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg
    56341 ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga
    56401 ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta
    56461 cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag
    56521 gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca
    56581 actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt
    56641 caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg
    56701 tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt
    56761 ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat
    56821 gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt
    56881 catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac
    56941 ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag
    57001 tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca
    57061 agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag
    57121 gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag
    57181 ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt
    57241 gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat
    57301 ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt
    57361 caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg
    57421 catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg
    57481 ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg
    57541 gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt
    57601 atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt
    57661 caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt
    57721 tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat
    57781 gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca
    57841 ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat
    57901 cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta
    57961 acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt
    58021 ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc
    58081 gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc
    58141 ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt
    58201 cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa
    58261 gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt
    58321 ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt
    58381 tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt
    58441 tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg
    58501 catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca
    58561 agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca
    58621 agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa
    58681 taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt
    58741 ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt
    58801 cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg
    58861 cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt
    58921 cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat
    58981 atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca
    59041 tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat
    59101 gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa
    59161 cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag
    59221 gaccaaaaat ccgctcacag aacaaccctt cacgttcagg ttttaaggta cgatagttaa
    59281 tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca
    59341 aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca
    59401 tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt
    59461 agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca
    59521 cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca
    59581 aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg
    59641 tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata
    59701 cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt
    59761 atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat
    59821 aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa
    59881 attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg
    59941 catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca
    60001 cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg
    60061 ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg
    60121 gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt
    60181 gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc
    60241 catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc
    60301 cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac
    60361 gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca
    60421 catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac
    60481 gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc
    60541 cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga
    60601 tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg
    60661 tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga
    60721 tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt
    60781 tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt
    60841 gttccataga tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa
    60901 cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt
    60961 atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa
    61021 cgacaccgtc atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca
    61081 taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt
    61141 tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg
    61201 caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt
    61261 ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat
    61321 tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt
    61381 cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa
    61441 atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc
    61501 cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt
    61561 cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt
    61621 gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa
    61681 agaattcttt gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg
    61741 tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga
    61801 tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt
    61861 cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa
    61921 tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt
    61981 ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat
    62041 ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga
    62101 aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct
    62161 cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca
    62221 aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat
    62281 cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac
    62341 gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat
    62401 gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg
    62461 tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac
    62521 ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc caagctccac
    62581 ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga
    62641 aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga
    62701 tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga
    62761 tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg
    62821 catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca taatctacaa
    62881 attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc
    62941 taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta
    63001 attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta
    63061 ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc
    63121 aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct
    63181 tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct
    63241 acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca
    63301 tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc aacacttcct
    63361 ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt
    63421 atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt
    63481 tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac
    63541 ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg
    63601 acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa
    63661 aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc
    63721 aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc
    63781 ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc
    63841 tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac
    63901 aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag
    63961 gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt
    64021 atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag
    64081 aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa
    64141 caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca
    64201 catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa
    64261 aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata
    64321 acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag
    64381 cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga
    64441 aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg
    64501 cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag
    64561 ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca
    64621 aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg
    64681 tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca
    64741 tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt
    64801 gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg
    64861 tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc
    64921 tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg
    64981 tcttctaggc tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa
    65041 attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac
    65101 atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg
    65161 ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc
    65221 tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg
    65281 tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa
    65341 gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca
    65401 aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc
    65461 tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt
    65521 cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga
    65581 accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca
    65641 gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac
    65701 gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat
    65761 gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga
    65821 aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag
    65881 ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa
    65941 tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa
    66001 gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa
    66061 ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat
    66121 cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg
    66181 atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac
    66241 cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc
    66301 actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc
    66361 atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca
    66421 gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa
    66481 gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc
    66541 ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac
    66601 tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca
    66661 acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag
    66721 ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa
    66781 aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta
    66841 ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa
    66901 acgatgttga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat
    66961 tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg
    67021 ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa
    67081 cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt
    67141 gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga
    67201 aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt
    67261 catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat
    67321 ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag
    67381 ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg
    67441 gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga
    67501 acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca
    67561 aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa
    67621 taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc
    67681 tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt
    67741 attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca
    67801 agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta
    67861 tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag
    67921 ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct
    67981 gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg
    68041 gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt
    68101 aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag
    68161 cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg
    68221 tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat
    68281 cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga
    68341 tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa
    68401 acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat
    68461 ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct aggataccta
    68521 cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt
    68581 tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga
    68641 acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag
    68701 gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa
    68761 gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg
    68821 tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta
    68881 actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg
    68941 tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg
    69001 gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt
    69061 ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg
    69121 gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg
    69181 atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg
    69241 agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta
    69301 aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt
    69361 tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc
    69421 cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt
    69481 ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag
    69541 atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca
    69601 gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc
    69661 aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc
    69721 ctaacgaaat atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag
    69781 atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DAFCLG010000004        68249 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFCLG010000004 DAFCLG010000000
VERSION     DAFCLG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.004"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..922
                     /locus_tag="LND56_000268"
     CDS             41..922
                     /locus_tag="LND56_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742222.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="LND56_000269"
     CDS             935..2545
                     /locus_tag="LND56_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBK6742223.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="LND56_000270"
     CDS             complement(2622..3281)
                     /locus_tag="LND56_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6742224.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="LND56_000271"
     CDS             complement(3564..4229)
                     /locus_tag="LND56_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBK6742225.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="LND56_000272"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="LND56_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBK6742226.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="LND56_000273"
     CDS             complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="LND56_000273"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK6742227.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="LND56_000274"
     CDS             complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="LND56_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBK6742228.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="LND56_000275"
     CDS             6543..6863
                     /locus_tag="LND56_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK6742229.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..7844)
                     /locus_tag="LND56_000276"
     CDS             complement(6975..7844)
                     /locus_tag="LND56_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6742230.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7940..9244)
                     /locus_tag="LND56_000277"
     CDS             complement(7940..9244)
                     /locus_tag="LND56_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6742231.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9241..9750)
                     /locus_tag="LND56_000278"
     CDS             complement(9241..9750)
                     /locus_tag="LND56_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742232.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9925..12060)
                     /locus_tag="LND56_000279"
     CDS             complement(9925..12060)
                     /locus_tag="LND56_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6742233.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12143..13798)
                     /locus_tag="LND56_000280"
     CDS             complement(12143..13798)
                     /locus_tag="LND56_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6742234.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13800..14114)
                     /locus_tag="LND56_000281"
     CDS             complement(13800..14114)
                     /locus_tag="LND56_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6742235.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14284..14544
                     /locus_tag="LND56_000282"
     CDS             14284..14544
                     /locus_tag="LND56_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBK6742236.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14558..14872
                     /locus_tag="LND56_000283"
     CDS             14558..14872
                     /locus_tag="LND56_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBK6742237.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15602..16156)
                     /locus_tag="LND56_000284"
     CDS             complement(15602..16156)
                     /locus_tag="LND56_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK6742238.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16282..18211)
                     /locus_tag="LND56_000285"
                     /pseudo
     CDS             complement(16282..18211)
                     /locus_tag="LND56_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18517..19233)
                     /locus_tag="LND56_000286"
     CDS             complement(18517..19233)
                     /locus_tag="LND56_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6742239.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19234..21057)
                     /gene="glpO"
                     /locus_tag="LND56_000287"
     CDS             complement(19234..21057)
                     /gene="glpO"
                     /locus_tag="LND56_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBK6742240.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21071..22567)
                     /gene="glpK"
                     /locus_tag="LND56_000288"
     CDS             complement(21071..22567)
                     /gene="glpK"
                     /locus_tag="LND56_000288"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK6742241.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22703..24163)
                     /locus_tag="LND56_000289"
     CDS             complement(22703..24163)
                     /locus_tag="LND56_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742242.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24153..25448)
                     /locus_tag="LND56_000290"
     CDS             complement(24153..25448)
                     /locus_tag="LND56_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6742243.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25780..26457)
                     /locus_tag="LND56_000291"
     CDS             complement(25780..26457)
                     /locus_tag="LND56_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBK6742244.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26487..27578)
                     /locus_tag="LND56_000292"
     CDS             complement(26487..27578)
                     /locus_tag="LND56_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBK6742245.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27679..28113)
                     /locus_tag="LND56_000293"
     CDS             complement(27679..28113)
                     /locus_tag="LND56_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6742246.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28697..30154)
                     /gene="ascB"
                     /locus_tag="LND56_000294"
     CDS             complement(28697..30154)
                     /gene="ascB"
                     /locus_tag="LND56_000294"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6742247.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30151..32046)
                     /locus_tag="LND56_000295"
     CDS             complement(30151..32046)
                     /locus_tag="LND56_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6742248.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32104..33879)
                     /locus_tag="LND56_000296"
     CDS             complement(32104..33879)
                     /locus_tag="LND56_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6742249.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33929..35425)
                     /locus_tag="LND56_000297"
     CDS             complement(33929..35425)
                     /locus_tag="LND56_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6742250.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35619..37241)
                     /locus_tag="LND56_000298"
     CDS             complement(35619..37241)
                     /locus_tag="LND56_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6742251.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37569..38882)
                     /locus_tag="LND56_000299"
     CDS             complement(37569..38882)
                     /locus_tag="LND56_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6742252.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39127..39351)
                     /locus_tag="LND56_000300"
     CDS             complement(39127..39351)
                     /locus_tag="LND56_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742253.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39371..39793)
                     /locus_tag="LND56_000301"
     CDS             complement(39371..39793)
                     /locus_tag="LND56_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBK6742254.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39935..40645)
                     /locus_tag="LND56_000302"
     CDS             complement(39935..40645)
                     /locus_tag="LND56_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742255.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40663..41160)
                     /locus_tag="LND56_000303"
     CDS             complement(40663..41160)
                     /locus_tag="LND56_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742256.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41197..41649)
                     /locus_tag="LND56_000304"
     CDS             complement(41197..41649)
                     /locus_tag="LND56_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6742257.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41784..42389)
                     /locus_tag="LND56_000305"
     CDS             complement(41784..42389)
                     /locus_tag="LND56_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6742258.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42839..43459)
                     /locus_tag="LND56_000306"
     CDS             complement(42839..43459)
                     /locus_tag="LND56_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK6742259.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43461..44330)
                     /locus_tag="LND56_000307"
     CDS             complement(43461..44330)
                     /locus_tag="LND56_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBK6742260.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44353..44760)
                     /locus_tag="LND56_000308"
     CDS             complement(44353..44760)
                     /locus_tag="LND56_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6742261.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44779..46068)
                     /locus_tag="LND56_000309"
     CDS             complement(44779..46068)
                     /locus_tag="LND56_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6742262.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46095..46397)
                     /locus_tag="LND56_000310"
     CDS             complement(46095..46397)
                     /locus_tag="LND56_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6742263.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46422..48515)
                     /locus_tag="LND56_000311"
     CDS             complement(46422..48515)
                     /locus_tag="LND56_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6742264.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49004..49768)
                     /locus_tag="LND56_000312"
     CDS             complement(49004..49768)
                     /locus_tag="LND56_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBK6742265.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49879..50748)
                     /gene="mdcB"
                     /locus_tag="LND56_000313"
     CDS             complement(49879..50748)
                     /gene="mdcB"
                     /locus_tag="LND56_000313"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBK6742266.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50773..51849)
                     /locus_tag="LND56_000314"
     CDS             complement(50773..51849)
                     /locus_tag="LND56_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK6742267.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51873..52466)
                     /locus_tag="LND56_000315"
     CDS             complement(51873..52466)
                     /locus_tag="LND56_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBK6742268.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52470..54146)
                     /gene="mdcD"
                     /locus_tag="LND56_000316"
     CDS             complement(52470..54146)
                     /gene="mdcD"
                     /locus_tag="LND56_000316"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBK6742269.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54136..54435)
                     /locus_tag="LND56_000317"
     CDS             complement(54136..54435)
                     /locus_tag="LND56_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBK6742270.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54537..56183)
                     /gene="mdcA"
                     /locus_tag="LND56_000318"
     CDS             complement(54537..56183)
                     /gene="mdcA"
                     /locus_tag="LND56_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBK6742271.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56190..57101)
                     /locus_tag="LND56_000319"
     CDS             complement(56190..57101)
                     /locus_tag="LND56_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6742272.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57221..58078
                     /locus_tag="LND56_000320"
     CDS             57221..58078
                     /locus_tag="LND56_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6742273.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58265..58627)
                     /locus_tag="LND56_000321"
     CDS             complement(58265..58627)
                     /locus_tag="LND56_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK6742274.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            58973..60412
                     /locus_tag="LND56_000322"
     CDS             58973..60412
                     /locus_tag="LND56_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742275.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60419..62971)
                     /locus_tag="LND56_000323"
     CDS             complement(60419..62971)
                     /locus_tag="LND56_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBK6742276.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63085..64416)
                     /locus_tag="LND56_000324"
     CDS             complement(63085..64416)
                     /locus_tag="LND56_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6742277.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64382..65860)
                     /locus_tag="LND56_000325"
     CDS             complement(64382..65860)
                     /locus_tag="LND56_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6742278.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66165..68000)
                     /gene="tdc"
                     /locus_tag="LND56_000326"
     CDS             complement(66165..68000)
                     /gene="tdc"
                     /locus_tag="LND56_000326"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6742279.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19418 a        14561 c        11675 g        22595 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcggattc gatcactgca aaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7861 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     7921 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     7981 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     8041 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8101 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8161 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8221 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8281 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     8341 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     8401 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     8461 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     8521 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     8581 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     8641 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     8701 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     8761 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     8821 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     8881 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     8941 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     9001 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     9061 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     9121 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     9181 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     9241 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     9301 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     9361 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
     9421 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
     9481 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
     9541 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
     9601 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
     9661 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
     9721 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
     9781 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
     9841 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
     9901 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
     9961 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    10021 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    10081 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    10141 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    10201 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    10261 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    10321 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    10381 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    10441 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    10501 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    10561 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    10621 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    10681 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    10741 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    10801 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    10861 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    10921 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    10981 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    11041 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    11101 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    11161 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    11221 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    11281 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    11341 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    11401 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    11461 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    11521 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    11581 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    11641 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    11701 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    11761 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    11821 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    11881 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    11941 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    12001 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    12061 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    12121 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    12181 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    12241 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    12301 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    12361 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    12421 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    12481 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    12541 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    12601 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    12661 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    12721 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    12781 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    12841 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    12901 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    12961 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    13021 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    13081 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    13141 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    13201 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    13261 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    13321 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    13381 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    13441 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    13501 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    13561 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    13621 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    13681 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    13741 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    13801 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    13861 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    13921 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    13981 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    14041 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    14101 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    14161 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    14221 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    14281 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    14341 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    14401 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    14461 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    14521 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    14581 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    14641 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    14701 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    14761 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    14821 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    14881 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    14941 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    15001 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    15061 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    15121 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    15181 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    15241 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    15301 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    15361 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    15421 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    15481 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
    15541 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    15601 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    15661 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    15721 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    15781 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    15841 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    15901 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    15961 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    16021 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    16081 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    16141 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    16201 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
    16261 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    16321 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    16381 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    16441 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    16501 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    16561 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    16621 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    16681 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    16741 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    16801 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    16861 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    16921 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    16981 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    17041 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    17101 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    17161 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    17221 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    17281 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    17341 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    17401 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    17461 atgccgctag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    17521 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    17581 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    17641 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    17701 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    17761 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    17821 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    17881 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    17941 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    18001 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    18061 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    18121 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    18181 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    18241 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    18301 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    18361 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    18421 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    18481 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    18541 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    18601 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    18661 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    18721 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    18781 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    18841 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    18901 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    18961 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    19021 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    19081 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    19141 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    19201 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    19261 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    19321 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    19381 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    19441 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    19501 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    19561 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    19621 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    19681 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    19741 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    19801 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    19861 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    19921 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    19981 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    20041 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    20101 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    20161 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    20221 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    20281 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    20341 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    20401 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    20461 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    20521 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    20581 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    20641 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    20701 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    20761 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    20821 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    20881 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    20941 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    21001 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    21061 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    21121 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    21181 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    21241 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    21301 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    21361 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    21421 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    21481 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    21541 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    21601 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    21661 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    21721 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    21781 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    21841 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    21901 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    21961 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    22021 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    22081 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    22141 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    22201 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    22261 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    22321 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    22381 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    22441 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    22501 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    22561 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    22621 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    22681 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    22741 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    22801 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    22861 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    22921 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    22981 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    23041 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    23101 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    23161 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    23221 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    23281 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    23341 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    23401 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    23461 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    23521 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    23581 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    23641 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    23701 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    23761 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    23821 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    23881 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    23941 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    24001 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    24061 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    24121 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    24181 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    24241 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    24301 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    24361 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    24421 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    24481 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    24541 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    24601 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    24661 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    24721 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    24781 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    24841 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    24901 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    24961 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    25021 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    25081 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    25141 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    25201 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    25261 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    25321 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    25381 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    25441 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    25501 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    25561 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    25621 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    25681 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    25741 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    25801 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    25861 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    25921 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    25981 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    26041 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    26101 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    26161 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    26221 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    26281 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    26341 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    26401 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    26461 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    26521 gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc
    26581 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    26641 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    26701 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    26761 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    26821 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    26881 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    26941 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    27001 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    27061 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    27121 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    27181 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    27241 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    27301 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    27361 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    27421 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    27481 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    27541 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    27601 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    27661 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    27721 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    27781 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    27841 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    27901 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    27961 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    28021 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    28081 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    28141 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    28201 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    28261 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    28321 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    28381 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    28441 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    28501 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    28561 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    28621 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    28681 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    28741 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    28801 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    28861 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    28921 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    28981 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    29041 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    29101 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    29161 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    29221 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    29281 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    29341 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    29401 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    29461 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    29521 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    29581 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    29641 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    29701 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    29761 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    29821 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    29881 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    29941 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    30001 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    30061 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    30121 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    30181 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    30241 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    30301 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    30361 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    30421 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    30481 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    30541 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    30601 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    30661 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    30721 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    30781 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    30841 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    30901 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    30961 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    31021 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    31081 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    31141 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    31201 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    31261 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    31321 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    31381 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    31441 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    31501 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    31561 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    31621 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    31681 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    31741 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    31801 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    31861 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    31921 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    31981 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    32041 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    32101 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    32161 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    32221 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    32281 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    32341 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    32401 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    32461 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    32521 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    32581 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    32641 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    32701 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    32761 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    32821 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    32881 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    32941 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    33001 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    33061 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    33121 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    33181 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    33241 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    33301 aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag
    33361 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    33421 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    33481 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    33541 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    33601 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    33661 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    33721 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    33781 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    33841 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    33901 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    33961 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    34021 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    34081 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    34141 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    34201 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    34261 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    34321 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    34381 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    34441 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    34501 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    34561 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    34621 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    34681 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    34741 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    34801 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    34861 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    34921 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    34981 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    35041 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    35101 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    35161 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    35221 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    35281 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    35341 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    35401 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    35461 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    35521 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    35581 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    35641 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    35701 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    35761 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    35821 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    35881 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    35941 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    36001 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    36061 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    36121 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    36181 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    36241 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    36301 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    36361 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    36421 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    36481 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    36541 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    36601 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    36661 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    36721 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    36781 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    36841 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    36901 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    36961 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    37021 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    37081 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    37141 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    37201 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    37261 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    37321 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    37381 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    37441 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    37501 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    37561 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    37621 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    37681 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    37741 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    37801 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    37861 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    37921 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    37981 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    38041 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    38101 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    38161 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    38221 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    38281 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    38341 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    38401 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    38461 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    38521 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    38581 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    38641 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    38701 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    38761 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    38821 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    38881 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    38941 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    39001 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    39061 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    39121 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    39181 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    39241 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    39301 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    39361 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    39421 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    39481 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    39541 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    39601 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    39661 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    39721 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    39781 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    39841 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    39901 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    39961 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    40021 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    40081 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    40141 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    40201 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
    40261 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    40321 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    40381 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    40441 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    40501 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    40561 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    40621 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    40681 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    40741 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    40801 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    40861 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    40921 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    40981 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
    41041 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
    41101 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
    41161 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
    41221 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
    41281 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
    41341 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
    41401 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
    41461 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
    41521 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
    41581 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
    41641 ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc
    41701 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
    41761 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
    41821 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
    41881 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
    41941 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
    42001 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
    42061 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
    42121 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
    42181 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
    42241 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
    42301 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
    42361 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
    42421 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
    42481 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
    42541 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
    42601 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
    42661 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
    42721 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
    42781 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
    42841 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
    42901 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
    42961 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
    43021 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
    43081 gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga
    43141 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
    43201 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
    43261 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
    43321 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
    43381 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
    43441 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
    43501 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
    43561 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
    43621 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
    43681 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
    43741 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
    43801 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
    43861 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
    43921 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
    43981 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
    44041 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
    44101 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
    44161 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
    44221 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
    44281 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
    44341 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
    44401 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
    44461 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
    44521 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
    44581 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
    44641 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
    44701 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
    44761 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
    44821 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt
    44881 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
    44941 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
    45001 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
    45061 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
    45121 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
    45181 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
    45241 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
    45301 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
    45361 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
    45421 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
    45481 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
    45541 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    45601 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    45661 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    45721 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    45781 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    45841 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    45901 gctcgaacca gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    45961 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    46021 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt ttttaccctc
    46081 ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat tttttcttca
    46141 atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg atcaactgcg
    46201 aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg tttcatggaa
    46261 actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt gttcaaaaca
    46321 tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa
    46381 atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc aactactgaa
    46441 aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc caacttctct
    46501 tcgttgttga tcaattcaat tagttctttc atgatgttca gatgcgagta ggagtctact
    46561 gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa atcgattggt
    46621 tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg tcgggcatgg
    46681 gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac tgcacgaatc
    46741 atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct agcagctagt
    46801 tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct gtccgtcaaa
    46861 atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt tgtttcgaat
    46921 aatttttcta acttgccata gatgattcga tctactcggc caccactttc tttcatgaca
    46981 tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa ccgctgtagt
    47041 tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt taaaatcgga
    47101 tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa aaccagattg
    47161 acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt aatctcgatc
    47221 tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat cccatggtta
    47281 cagacaacga ctgatttata aacataattc aagtctcgct tgattacact ggcgatcgtc
    47341 gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc aattacttct
    47401 tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt tgttcggatg
    47461 ttttctttca atggattcat gacttggata tgattcttga ttcttacaat cagtgcagcc
    47521 atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg tacgtgtgtc
    47581 tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac ccactcgatt
    47641 gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata atttctttct
    47701 acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag
    47761 ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc atgtttcctg
    47821 attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat atcatcttct
    47881 tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac caaaggaaaa
    47941 taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat aaagaagtcg
    48001 gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga tcgttctgaa
    48061 cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata actgaccagt
    48121 gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg aaattcttct
    48181 tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa
    48241 atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt ttctaatcga
    48301 gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt acggatctcc
    48361 ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg gatcgtccgc
    48421 tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg ggctgtcatg
    48481 attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa
    48541 gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa gtggaaagaa
    48601 agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa cgaaaaagac
    48661 atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg tgaaaaagct
    48721 gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg
    48781 ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag gttgtgacag
    48841 agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca tattttacaa
    48901 aatcgtgcct tattgccgat aggctatctc tggcacaccg tttattcaga aaaaggagct
    48961 gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc agcataatcg
    49021 gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa taccatatct
    49081 gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct tttcgatcga
    49141 ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg tggatctgtc
    49201 gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca ccgtctgctt
    49261 tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa cagcttctgg
    49321 ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc ttttggattt
    49381 ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat atctccgctt
    49441 tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc cctcctactg
    49501 acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct gtacctggac
    49561 cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg cccccatatg
    49621 aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct ccaccatgac
    49681 cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc actaacaaaa
    49741 tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat tacaaaaaac
    49801 tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata aactgttttt
    49861 ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg tgtcggcagt
    49921 ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg cgcttcctcc
    49981 aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac gatttggtaa
    50041 agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt tctgatcact
    50101 gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca tatgcatttg
    50161 tttctgtaca tatgaatcat tatctagctc cttagctgct tgaattgcaa gcctgatatg
    50221 cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt gcttcaccct
    50281 atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag caagcttgct
    50341 gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt tttgtgcaat
    50401 gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga tgccatttgt
    50461 agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt ctcttaactc
    50521 ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag gagttagcgt
    50581 cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg catccagtgc
    50641 atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt cctctaccaa
    50701 cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga cactcactcc
    50761 ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga tatgaagtaa
    50821 ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa tcatgactcc
    50881 tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga aaacaaatac
    50941 attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg gcaattttgg
    51001 cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga tgactaaact
    51061 gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt ctcctggatg
    51121 tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta agaaaagata
    51181 acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg ctaccccata
    51241 aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat aaggcccagt
    51301 caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg aaccaatcaa
    51361 tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg
    51421 catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg ctccatcacc
    51481 tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt gttggactgc
    51541 tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa
    51601 cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca tattataaac
    51661 aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa
    51721 agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat
    51781 aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga tttttttgtt
    51841 ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt taatttaggt
    51901 ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc ttttagtgaa
    51961 aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc aggatgcgat
    52021 tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca
    52081 ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct gccgccgatt
    52141 ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata aacaggataa
    52201 tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg atgaatgact
    52261 tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc aggtccacgt
    52321 aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc
    52381 ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa
    52441 aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc cattttggca
    52501 cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt gtatcgattt
    52561 gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat tgcttcgatg
    52621 actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag gaaatgatag
    52681 atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac tttttcagca
    52741 gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc tttagacatc
    52801 gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg taatccatgt
    52861 gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg atgtccagct
    52921 tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat accgatcaat
    52981 tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat tggccgtttc
    53041 acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct
    53101 tcaactaaac caacttcacc gtttcgtaca cggtacatcg ggtttttagg atcaggtacg
    53161 attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata cacagtacta
    53221 atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta accaacactt
    53281 gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt tgcttgatgg
    53341 ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc taaagaatca
    53401 agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt
    53461 gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc atcaatgatt
    53521 tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt gtcactagaa
    53581 tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa accgactctc
    53641 gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat accgccaaat
    53701 gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag agcaatgatt
    53761 acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa acgaacacca
    53821 cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc ttctaacaca
    53881 tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc accttggaat
    53941 tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg gctgacaacg
    54001 atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc gatgatttga
    54061 tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg ttctctggcg
    54121 tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg tcaatcgtag
    54181 gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg aaattggata
    54241 acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat cgcttcctgt
    54301 acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt ccaaatctcc
    54361 tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg gatatttata
    54421 agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac gttctgctta
    54481 ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg ccaattttat
    54541 gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc gatcaaatca
    54601 tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat gcctagatct
    54661 tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt cgtgctagtc
    54721 attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc ttcttttgtt
    54781 tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt atcttcgcta
    54841 taaatcatca ctggtgcatt ggctaagcct gcttgttttt gaacagctag tgcatctaat
    54901 tcttcgacaa agactggttt tttatttgaa ccatatgttt caaccatttg aacaactaat
    54961 ttttggcctt tccctaatgg atcatcattg tcgcgaagtg attgccatgc tggtgtagaa
    55021 tgacgacgac cgccaggatt atgtcccatg ttaggcgcac cgccaaaacc agataaacgt
    55081 ccttttgtta cggtagaaga gtttccttcg taatctaatt gcaaggttga accgataaac
    55141 atatcgatgg catattgtcc tgcagcttgt gataatgttc ggttactgcg catcgtgccg
    55201 tctttaccga caaaaaagac atctgggcga gcagcaatat atttttccat accaacttcg
    55261 ccaccaaaac tatggatgct ttcaacccaa cctgattcga tggctggaat catcgttgga
    55321 tgtgggttca atacccagtt cttcgcgatt ttccctttta accctagtga ttcaccataa
    55381 gttggcagta acaattcgat agctgctgta ttgaatccaa taccgtgatt caaagattgt
    55441 acgccgtgtt ctgcgtagat tcctttgatt gccatcattg ccattagaat ttgcaattct
    55501 gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt gataaggttt atctgctttc
    55561 acgacaacgt ctacccagtc accagggata tctacacgtg gtaaagaatc gacaacttcg
    55621 ttaacttgaa cgatcacgat accatctttg aaagctgcag cttcgatgat tgttggtgtt
    55681 tcttccgtat tgaaacctgt gtacaagttg ccgtctttat ctgctttatc tgcagctact
    55741 aaaacaacat ttggaatcaa atcgatgaat aagcgtccat aaagttccaa ataagtatgg
    55801 atatcaccta atttcattgt gccgtcttca atcatttggg cgacacgcaa gctttgtgca
    55861 ccagcataag agaaatcgat cttgcttgcg attccttttt caaaaatatt caaatgttct
    55921 ggtcgtgaga tactagacat gatcaaatgc agatcattca ctttttctgc atctactttt
    55981 tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt cgccttctaa tacaactttg
    56041 tcgccgggag caatcagttt ttccattact tctacgattt tttcagtttc tacatacttg
    56101 ccattgagca agttgctgat tttttccaat cgctgcgctt ttttttcgcg tttagaagcc
    56161 cagttgcggt ttggtttttc cataattagt catctccatt tttgatatag aaacgtggcg
    56221 tcatcaatac catgttggtc tagattgatc gtacggatcc ctttatctac accaaatttt
    56281 gtatgaatca gctttgtcaa agtattgccg gggggaaatt caatcgtcag atccattcct
    56341 aattcttttg ccaagtccat catttggttc cactgcacag ggaaagctag gttttccaac
    56401 aaatctttac gaatctcttc tgcgtcctga gtggctcttc catccgtatt ttttatatac
    56461 aaacatctag catcagatag atggacagac tgcatatacg gcttgaattt cttgaccgta
    56521 tcattcatca agagacagtg agatggcaca gggactttca gcagcttcgc tgtttgcgct
    56581 tggtgccgtt tggcttctgc caacgtaacg gataaacctt cgatactccc agataacgta
    56641 tgttgcatcg gacaattttc atttgacaga taaactggat ggtcaggatc aaatgttttt
    56701 gctacaattt cctcagcttc gcttctcgtc acacctacta ctacgcccat tgcataaccg
    56761 cttggataag cttctttcat taagactgcg cggtgataga ctagttccgc tgcattctca
    56821 aatctcaaag atcgacaagc aacagctgca caaaaagcac caatcgaatg tcctgctgca
    56881 atatcaggct gaataccgtt tttttccatt tcatctaaat aaaatactgc ttttgtcagt
    56941 aatgctagct ggataaaaac cgtatcttga tagttttctt ctttatcaac taaccgatac
    57001 cccgtcacat tttctaaacg ttccatgtac atttcaggga tatccgctaa catccctaac
    57061 ttctgactgc cttgtccagg aaataagaaa cctgtactca tgagcctcgc ctcctcagca
    57121 cttacttttt ccactgctat cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag
    57181 aaaaaaccat aacaaaaagg tatggatggg atggataatt atgaaattgc ttcatctgca
    57241 gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa gcagccaaag aattatttat
    57301 ttctcaacct gccttgagcc gtgcgatcaa acatttggaa aacgaattag gtgtcccttt
    57361 gttttaccat agcggcagaa acattgagac gaccgtttat gcagaagaat tttatccata
    57421 tgcagtcaag acattagaca cattagaaga aggaatcaaa ttattgtctt cggtcaatga
    57481 gaagatcgtt acttccgtca tattgtattt agaggtagcc tctgtttcta ttccgaactt
    57541 ggtccgaatt tttatggaaa agcatcctga tatccaatta acgatcatgc aacatcattt
    57601 accagatgat attaccgatc ctgtactgta catcacttcg gagatgaagc ctggcatgac
    57661 caatattcct atcatgaaag aacctgttta tgtcgccatt cctaagaaac atcctcttgc
    57721 taaaaaagaa catctgcaat tagaagatat ccaacgcacc cccatcttga tgctgtcaaa
    57781 gagcaacgcc ttccgtcata cactggatgc agcacttgaa gcgaaaagta tcgaattgac
    57841 gatcagttcg acgacagatg atcctgcgac attacgttct attgtcaagc aaggattagc
    57901 tgttagcttt ttcccaaaag tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg
    57961 ggaaatcaaa gatttgccct tgacacgaac aatttattta tcttctagct tcacagaaga
    58021 tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa
    58081 aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag
    58141 aagtaatttc ttagaaaata agccgaaatt tcccaaaaat ttgagaagca attttcgtaa
    58201 attccttctt atttcttgaa gctaaacatt tctgtcacaa cctcttctct attgtcctag
    58261 acattcattg cgttgattta aaacttccat caatcaaatc tgctagttca tctagctttt
    58321 ctattttacg tagatcaata gagatccaat gttctttatt catgtgatag gctggaaaat
    58381 acccctcttt tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa tcaagaatat
    58441 tgatgatcct atggtcactt gcatcatcat agattttttc ttctggaaca gacatcagga
    58501 ccccaaacca ttttttgctt ttttgatgtc tgaatacgca ataatcagga aattttttaa
    58561 aaatatattc tggagacaaa ttgtatgttt cattgatgtg atcgattagt tcttttttcg
    58621 ttagcatctg cttccatctc tttcttattg attcgcttta ttcaaagata ccacgtaaga
    58681 atagaaaaat gaatacaagt agttgctaat caaatacttc gatatttttc ttttttatca
    58741 actcttaaat gttcaatccg cctgtgttgg tcttgctacc atctactatt aaaatgttta
    58801 ttattgtttg cccacctatt ttttgatttt agtggtatca tttttcaagt tcatgatgaa
    58861 attcttatca tcagtaaatg ctccagaaaa ataaacaatg attgaaaaat cagttgaaac
    58921 ttgaccaccg tttattttct ccgtattctt agaatagaag ggagtctgat tgatgtattc
    58981 actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt catgtttctt tagtagaaca
    59041 attgctgaat catacaaaga taaccgtaaa tgaattagct gaaatcatag ggacaaccga
    59101 acgaacgatt ttttctgacc ttcaatctat tcgctctcag ctgcccgaag gctgggacat
    59161 catcagcgat caagcaggaa tcagtttgca aaaccagcaa aatttattaa caaatgacct
    59221 ttgggagatt tttttcaaac aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac
    59281 aaaaaaagtt gctgtccctg attttctagc cgaccatggg ctttcttatg ggacattgaa
    59341 aagacatgtg actaaaatca atcagcgttt agcaagttac gacttgcaga tcgatttgac
    59401 gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt
    59461 gctgattcct ttcactcata acaactattt tttcgaggat tattcgatcc atgaatccca
    59521 ttattttcaa tttttaagaa acctcagtca aactgaacta gcggttgaaa cagaagagat
    59581 tttcggaaca tgttggttct ttatcaatac gatccgtata aaagcgaatt gccgattaga
    59641 ttcttctatc cataccaaca gcacactctc ctccctgtat gactctgcat tgaaaaaatt
    59701 gtatttaacg gaaggtattt atcttaaaga cactgagctt tcttttgctt ctttctgctt
    59761 tctggaaagt tggaattaca acaataacta tggacaagaa accgctcgct gcctgcatca
    59821 ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct
    59881 tgatcaacta aaaaaaacat ctctgactga taacttggct ctcctcatat taaaatatca
    59941 tgaatcgccg atattgattg aagcattgga ccgtcaatac catcgttttt tagaaacata
    60001 tgatcagcaa tatgcacact tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa
    60061 agaaatagaa atcaatgaac caaattactt tctccaactc ttgtctctac tgatccaaca
    60121 aacgattttt tctatcaaac cgaatttgtt caatgtctat tttattttcc aaggagaacc
    60181 ttcatggaag gcgttcttgc agcaggaatt aaaagattac ttaggaaaac gtgtctgctt
    60241 cttacctatc gaactaaccg atctttccac tatttctttt gaaaaaacag atatcttgat
    60301 ttccaatttc cctctagatt ctctagatat ccctattgta tacatttctt ctattccaac
    60361 gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa acttttttat agtaacggct
    60421 agcgactgta ttctctagct gttttttctt ttttgcgaaa cagtttgttc atgatccctt
    60481 tttctatttc tacaactaag aatagaagaa tcacatttgc tccaatcaat aactgttgtg
    60541 tcatcgttaa acttactgta ccgatcagct gctggccgac tggtagaaaa ataaccatcc
    60601 cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt
    60661 ggttcaatga cggcttggac aactctctgc aattgatcat gtaaacagct tgagctaaga
    60721 cgatattttg cagaagaatg gtttgctgta agctttgccc ttcaaactgc atagccaata
    60781 aataggcagg aaccatgatc agtagggaga catagatgat ccgaaacaaa tgataaccag
    60841 ataggattcc ttgctttgga tctcttggtg ggcgcttcat gatttctgga tctggcgact
    60901 cgaaccctag tgcataagat aaagtgattg tcgttaccat attgacccat aaaatctgta
    60961 ctggggacaa aggtagcggg cgattcagca taagtgccca aatcaagatc aatccttgag
    61021 ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa attatccaaa atccgccggc
    61081 cctcttttac ggcttttgca atcgtatgga aattatcatc tcctaaaacc atatcagctg
    61141 cctgtttgct tacttcacta cctttgattc ccatagcaat tcctacatcg gatcttttca
    61201 aagcaggtgc atcatttacg ccgtctccgg tcattccgac gatttctcca tttttctgca
    61261 aagctttaac aattcgcagt ttatgttctg gtgtcgttcg agcaaagaca tcgactattg
    61321 gcacttgctg aatcaactct tcatcagaca taagatccaa atcgatccct tctaaaactt
    61381 tggcagtatg ttttaagcca atttgttccg caatcgcttt tgctgtgtct ttgtgatccc
    61441 ctgtaatcat tttcactgag atccctgctt gttggcattc ctttacagct ttgactgcac
    61501 tttcttttgg cggatcgatc aatccggcga tccctgcaaa agttaattgg gttaaagtct
    61561 catgcgtgag ttcctgtttc gaatctactt ttttataagc aaatcctaaa acacgctgac
    61621 cttttctcgc aaattctgca gctgtctgac tccatgcttc ttgttctgca gaagaaagag
    61681 ttgatttagc aaataatact tctggtgcac ctttgacgta aatcaccgct cctttttcct
    61741 ccgcatgcat cgttgccata tatttgtatt cagaactgaa cggaatcttt gcttctattt
    61801 ttttcaaagg tagattggct gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc
    61861 cacttaaatt ttctgttttc tgttcttctt ttgtttttaa ttcctgacag tttttcataa
    61921 tggacaacac gcaggcttct tcttttgcag ctacatccac aactgtcatt tcattttttg
    61981 tcaatgttcc tgtcttatct gaacagatca ctgtcataga acctaatgtt tctaccgaag
    62041 gaagtccttt gatgatcgcc ttttcttttg ccatttcatg tactccaacg gatagaatca
    62101 tggtcaaaac agctggcaat ccttcgggta ccattgacac caccaaagca atcgtagccg
    62161 aaagaagaat atgccaatcc ataccatatc gaaatgtcgt aaaaataact aagaataggc
    62221 tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg gttgattttt ttaatcagag
    62281 gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat tttaccgatt tctgtcgtgt
    62341 ttcctgtttc tacaacgaca ccgactgcag aaccagattg tacaagcgtt cctgaaaaac
    62401 ccatattttt ctggtcgccg atcgattctt cttctgtcaa aacggcatga cttttttcag
    62461 atgcttctga ttctcccgtc aaaatcgatt cttccactaa taaatcatgt gcttcgatta
    62521 atcggatatc tgctggaacg acatctccgg cacgtaagac aatgacatct ccttttacca
    62581 gttctttcgc agggattttt tcttgcatcc cgtttgatag tactatcgct tcttgtccca
    62641 tcagctgctt caaaccattc aatgattctt ccgcttttct ttcttgccag taactaacga
    62701 gtccattgac gatcactacg aataaaataa tacttccttc aatatattca ctagaagcaa
    62761 atttcaaaaa agaggcaatg atcaacacga tcatcagcag atcagtaaaa tgctttgcta
    62821 tttttttcca tcgttttgtc tgctgccttg cttttatttc attatatcct tgatcagtca
    62881 ggcgtttctg tcgttcttct tgcgacaagc cttctgttga tgtttgatag tttttcaata
    62941 catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa ttgatactgt
    63001 tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt ctgaaataat
    63061 tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc ttttatatac
    63121 aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg tcgtgctttc
    63181 gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat ctttatatct
    63241 ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca agatcgaaga
    63301 ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact
    63361 atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg taattaaaat
    63421 accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca aacttttttc
    63481 ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg ctgaggacaa
    63541 agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat ggatcacttg
    63601 cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa caacaaataa
    63661 aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac agttgattgc
    63721 ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga tcatttcaat
    63781 tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa agccaaacgg
    63841 tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg tatgaaaatc
    63901 tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga tcttgatgat
    63961 tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga ctgccattaa
    64021 gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc agaaatggat
    64081 atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa gccaataagt
    64141 ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat cactgataga
    64201 gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac cataaatagc
    64261 caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa ttgcctttgc
    64321 tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact ggatatttcg
    64381 attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt tctttttcaa
    64441 gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata cagagtggta
    64501 ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat gttccagctt
    64561 tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg acagtccaaa
    64621 ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc aactttgcat
    64681 gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata cagtagataa
    64741 cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc agcaaataaa
    64801 aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg gatacgtcaa
    64861 attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta gcactttctg
    64921 tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca ccaatgaaga
    64981 caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg caataaatcg
    65041 aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca tttgctacaa
    65101 ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat actccgcgaa
    65161 tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt tcgataccag
    65221 taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta gtatctggta
    65281 ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca actttgatcc
    65341 acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct aaccaaacac
    65401 cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg atgatccaat
    65461 aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc acattatcga
    65521 taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca ggaaacattt
    65581 ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca ttcgataccc
    65641 aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag atcaatgaga
    65701 ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg
    65761 ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt ccgataaagg
    65821 tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct tctttcaata
    65881 gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac aaggtttcat
    65941 cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta gtgaactggg
    66001 tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag aatatcttgg
    66061 cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa ataattgtct
    66121 gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg gatgatttcg
    66181 tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa aattcgatca
    66241 tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg ttcttttttc
    66301 cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc accgtaatct
    66361 tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc gccatccata
    66421 taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa atctgtacag
    66481 ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa tggatagact
    66541 tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct gaatctttga
    66601 gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa tggcaataca
    66661 cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc caaaatataa
    66721 gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc gaaataagaa
    66781 atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata agggacatag
    66841 cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct gaacccttca
    66901 tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc aaagaattca
    66961 gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga acgcgcatag
    67021 ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt taacctttct
    67081 cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt tcctacgaca
    67141 gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt tttttccaaa
    67201 gcattaatat ccattcgata atcctcttga actgggatag caaccatctg atctaagccg
    67261 actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg gaccagccat
    67321 ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac ctcatccatt
    67381 tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa attcaaaagt
    67441 tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc taaaggaatc
    67501 gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc atctgctgca
    67561 atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc tacttctgct
    67621 tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt ccacagcatc
    67681 gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg tccccagtaa
    67741 cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac ttcatccaag
    67801 acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc tccttcgcta
    67861 attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc atctaccatc
    67921 ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat aaagacggcc
    67981 ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat gtcttgcaga
    68041 tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa tttttttttt
    68101 ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg atttttctga
    68161 taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa atagataacc
    68221 taaaaaaagc gaacaaacaa ataaaatga
//
LOCUS       DAFCLG010000005        68078 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFCLG010000005 DAFCLG010000000
VERSION     DAFCLG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.005"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            20..619
                     /locus_tag="LND56_000327"
     CDS             20..619
                     /locus_tag="LND56_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6742280.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            complement(688..1989)
                     /locus_tag="LND56_000328"
     CDS             complement(688..1989)
                     /locus_tag="LND56_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6742281.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     misc_binding    2252..2457
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2569..3825
                     /locus_tag="LND56_000329"
     CDS             2569..3825
                     /locus_tag="LND56_000329"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK6742282.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            4116..5993
                     /gene="tdc"
                     /locus_tag="LND56_000330"
     CDS             4116..5993
                     /gene="tdc"
                     /locus_tag="LND56_000330"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6742283.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            6236..7657
                     /locus_tag="LND56_000331"
     CDS             6236..7657
                     /locus_tag="LND56_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6742284.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            7766..9133
                     /gene="nhaC"
                     /locus_tag="LND56_000332"
     CDS             7766..9133
                     /gene="nhaC"
                     /locus_tag="LND56_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6742285.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(9414..10433)
                     /locus_tag="LND56_000333"
     CDS             complement(9414..10433)
                     /locus_tag="LND56_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6742286.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(10455..11462)
                     /locus_tag="LND56_000334"
     CDS             complement(10455..11462)
                     /locus_tag="LND56_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6742287.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(11455..12120)
                     /gene="pgmB"
                     /locus_tag="LND56_000335"
     CDS             complement(11455..12120)
                     /gene="pgmB"
                     /locus_tag="LND56_000335"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6742288.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(12113..14407)
                     /locus_tag="LND56_000336"
     CDS             complement(12113..14407)
                     /locus_tag="LND56_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6742289.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14667..16832
                     /locus_tag="LND56_000337"
     CDS             14667..16832
                     /locus_tag="LND56_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6742290.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            17136..17969
                     /locus_tag="LND56_000338"
     CDS             17136..17969
                     /locus_tag="LND56_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK6742291.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(18071..19771)
                     /locus_tag="LND56_000339"
     CDS             complement(18071..19771)
                     /locus_tag="LND56_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742292.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            20278..21438
                     /locus_tag="LND56_000340"
     CDS             20278..21438
                     /locus_tag="LND56_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6742293.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            21454..21897
                     /locus_tag="LND56_000341"
     CDS             21454..21897
                     /locus_tag="LND56_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742294.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(22380..22910)
                     /locus_tag="LND56_000342"
     CDS             complement(22380..22910)
                     /locus_tag="LND56_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBK6742295.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            23015..23566
                     /locus_tag="LND56_000343"
     CDS             23015..23566
                     /locus_tag="LND56_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6742296.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(23719..24192)
                     /locus_tag="LND56_000344"
     CDS             complement(23719..24192)
                     /locus_tag="LND56_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6742297.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            24330..25802
                     /locus_tag="LND56_000345"
     CDS             24330..25802
                     /locus_tag="LND56_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6742298.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            25799..26509
                     /locus_tag="LND56_000346"
     CDS             25799..26509
                     /locus_tag="LND56_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBK6742299.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            26861..27145
                     /locus_tag="LND56_000347"
     CDS             26861..27145
                     /locus_tag="LND56_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBK6742300.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(27426..27845)
                     /locus_tag="LND56_000348"
     CDS             complement(27426..27845)
                     /locus_tag="LND56_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBK6742301.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(27847..28431)
                     /locus_tag="LND56_000349"
     CDS             complement(27847..28431)
                     /locus_tag="LND56_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK6742302.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(28478..29953)
                     /locus_tag="LND56_000350"
     CDS             complement(28478..29953)
                     /locus_tag="LND56_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK6742303.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            30134..30445
                     /locus_tag="LND56_000351"
     CDS             30134..30445
                     /locus_tag="LND56_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742304.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(30561..32897)
                     /locus_tag="LND56_000352"
     CDS             complement(30561..32897)
                     /locus_tag="LND56_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6742305.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            33039..33707
                     /locus_tag="LND56_000353"
     CDS             33039..33707
                     /locus_tag="LND56_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6742306.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            33710..34753
                     /locus_tag="LND56_000354"
     CDS             33710..34753
                     /locus_tag="LND56_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6742307.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34990..35871)
                     /locus_tag="LND56_000355"
     CDS             complement(34990..35871)
                     /locus_tag="LND56_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6742308.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELE
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            36030..36758
                     /locus_tag="LND56_000356"
     CDS             36030..36758
                     /locus_tag="LND56_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK6742309.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            36914..37231
                     /locus_tag="LND56_000357"
     CDS             36914..37231
                     /locus_tag="LND56_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6742310.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            37224..37832
                     /locus_tag="LND56_000358"
     CDS             37224..37832
                     /locus_tag="LND56_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBK6742311.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            37887..38678
                     /locus_tag="LND56_000359"
     CDS             37887..38678
                     /locus_tag="LND56_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBK6742312.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            38816..39304
                     /locus_tag="LND56_000360"
     CDS             38816..39304
                     /locus_tag="LND56_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBK6742313.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            39355..40227
                     /locus_tag="LND56_000361"
     CDS             39355..40227
                     /locus_tag="LND56_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6742314.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            40403..40765
                     /locus_tag="LND56_000362"
     CDS             40403..40765
                     /locus_tag="LND56_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6742315.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            40774..41526
                     /locus_tag="LND56_000363"
     CDS             40774..41526
                     /locus_tag="LND56_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742316.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            41540..43342
                     /locus_tag="LND56_000364"
     CDS             41540..43342
                     /locus_tag="LND56_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742317.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            43619..44659
                     /locus_tag="LND56_000365"
     CDS             43619..44659
                     /locus_tag="LND56_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6742318.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(44711..46855)
                     /locus_tag="LND56_000366"
     CDS             complement(44711..46855)
                     /locus_tag="LND56_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6742319.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(47212..48585)
                     /locus_tag="LND56_000367"
     CDS             complement(47212..48585)
                     /locus_tag="LND56_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6742320.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(48585..48980)
                     /gene="rbsD"
                     /locus_tag="LND56_000368"
     CDS             complement(48585..48980)
                     /gene="rbsD"
                     /locus_tag="LND56_000368"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK6742321.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(49008..49925)
                     /gene="rbsK"
                     /locus_tag="LND56_000369"
     CDS             complement(49008..49925)
                     /gene="rbsK"
                     /locus_tag="LND56_000369"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK6742322.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(50033..51046)
                     /locus_tag="LND56_000370"
     CDS             complement(50033..51046)
                     /locus_tag="LND56_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6742323.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(51127..51726)
                     /locus_tag="LND56_000371"
     CDS             complement(51127..51726)
                     /locus_tag="LND56_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6742324.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            51912..52613
                     /locus_tag="LND56_000372"
     CDS             51912..52613
                     /locus_tag="LND56_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742325.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            52623..55334
                     /locus_tag="LND56_000373"
     CDS             52623..55334
                     /locus_tag="LND56_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742326.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            55785..57413
                     /gene="treC"
                     /locus_tag="LND56_000374"
     CDS             55785..57413
                     /gene="treC"
                     /locus_tag="LND56_000374"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK6742327.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(57473..58654)
                     /locus_tag="LND56_000375"
     CDS             complement(57473..58654)
                     /locus_tag="LND56_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6742328.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            58906..60429
                     /locus_tag="LND56_000376"
     CDS             58906..60429
                     /locus_tag="LND56_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK6742329.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(60498..61226)
                     /locus_tag="LND56_000377"
     CDS             complement(60498..61226)
                     /locus_tag="LND56_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6742330.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(61239..62519)
                     /locus_tag="LND56_000378"
     CDS             complement(61239..62519)
                     /locus_tag="LND56_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6742331.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(62660..63262)
                     /locus_tag="LND56_000379"
     CDS             complement(62660..63262)
                     /locus_tag="LND56_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6742332.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            63507..63713
                     /locus_tag="LND56_000380"
     CDS             63507..63713
                     /locus_tag="LND56_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742333.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            64025..64744
                     /locus_tag="LND56_000381"
     CDS             64025..64744
                     /locus_tag="LND56_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6742334.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(64825..65508)
                     /locus_tag="LND56_000382"
     CDS             complement(64825..65508)
                     /locus_tag="LND56_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBK6742335.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            65726..67912
                     /locus_tag="LND56_000383"
     CDS             65726..67912
                     /locus_tag="LND56_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6742336.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        21731 a        12124 c        13811 g        20412 t
ORIGIN      
        1 attacgggag gctctacaaa tgaacggttt tgaaaagaga gcacaagaga aaaagaaaga
       61 gattttacaa gcagcttttg acttgatgaa cacagacata ggaaccgctg gagtgacaat
      121 ggataaggta gctgagaacg ctcaggtaag taaagcgacg atttttaaat attttggcag
      181 caaggaaggg ctgatttatg aagtattttt tgcatttatg aatgatttag ggcaagaagc
      241 gaaagcgttg attgcggaaa atcttccatt tgaagaaaca ctgattgcca tgagtgaaaa
      301 caaaatccgt tatctcaatc gagtgaacaa acaattttat atcgatttga tgacatacta
      361 tacaaaaaaa gaaaaaaaag aattttctga cgcgatggat gcgtatacgc aagaaagttt
      421 cagtatcatg cttgatcttt tccatcgagg gcggaaagaa ggaaaagtgg atttgaaata
      481 ttcggatgag ttcttgatcc tctattttca agcaatggta gaaggaattt ctaagccgga
      541 gatatacgca aaactgcttc catatacagc agaatggaca gaagtcctga tcaagggaat
      601 cgcaccgaag aagaaataaa gcataaaaaa gccgaaactg acgataaaat gtcagttcgg
      661 cttttttggt tcataaatga cgtaaaatca agcagattcg cgtgcttctt tgtcatagac
      721 atcatggaca ttgatgaatg gatcaagaat aggatttttt ccgtctaaat cagattgaaa
      781 acgaatgcca tcgatattga tccgagcatt tcgatagaaa tccgtaccta attgtcccca
      841 agctggtgta gaagcatcga cattacagaa aatatcgaaa ccttgactct tcaagtaagc
      901 aaatttttga ttttctgctg aataaggctg ccagtcgctg atgtctgcac caaatggata
      961 aatcagaata tttgtttttc ctaggatagg tgcaacttct tgctgccaca attcattgtc
     1021 tttttttaca tcttctagtg aagcttgagt catattcaag tgaccccatg tatgggaagc
     1081 aaagctccag ccgtctttct tcaactgttc cgccactttt ttagcttctt taatctcttt
     1141 ttttgttttt tcattgtctc catattgaga ttttgatgtt cgataaccta aaactccgtt
     1201 gtaacctgtc agggccaagg taccttttgc gcctcgataa gaaaaatcag gatgtttctt
     1261 gacaaaggca tcgatcagag gaaccatgtc aaagtcaccg atttcttctt tatctttctc
     1321 tttatagaca tttttgattt cattgttatt tgatacgacc aatcggctag gaaaaccgga
     1381 attatccata tattcataat agctgacatc atcttgtgaa agaaccaatg gtttttttcc
     1441 ttcaggtagc gcaagcccgt taaaggaaag atttccttgt tcgtcttttt ggatcaaatc
     1501 attcaaatta actaaaacaa aatcattttg ataaagttgt tcgatcgaac ggttgaattc
     1561 ttcaacagtc accatataat ctttgtatcc ttgtgcctgt tgagagtgga aggctttttg
     1621 cgggtccacg atcaagctgt ggaaaaagac atgagagatc tgagtaggat cagaccagtc
     1681 gatcagttgt tctttctcct tttttagtgt ttgaagaagc tgttgtgttt cagtagatcc
     1741 gttcttacta tctttttcca ataacgcaat cgcttggtca taatcgtagc tttgagccag
     1801 attttttgct tgagtaacta gttgttgctt atcatctgtc ggttcttttg ctttttctgt
     1861 tatttttttt tcaggaatga ttggttcttt ttttgtagat gatcggatga caaaaaacaa
     1921 acttagaatt aaaataacga tagttaaagc agaagtggct cctaaaataa ttttttgctt
     1981 tttttccata tacctcgtcc cttgcttttt cattttttta cttgtcccat tccttattgt
     2041 aacgaagaaa gaataagagg caaagtaaag tgacttctcg ataaattgca gaaaaaatac
     2101 agaaaaagtt atttctttta acaataattg aaaataaaat gttcggaatt agagcggatt
     2161 caaaaaagaa aaaatgagga atttaaaaaa aatatccttt ttctgttgac aaagaaaaag
     2221 ttgcgctcta tgatagacac atattaataa taacaagacg aagatcgaaa cgaagtagtt
     2281 gttaaagcca atgtttcaga aagctggtgg tcgctgcaaa ccagtacatg ctttaaagcg
     2341 aattacattt caggagtctt tggcagtgat tgccacgcga cagcgttaaa tgttcgagag
     2401 gtatgttgga tatctagaca tacaaattgg gtggtaccgc gagatttcgt cccttggctt
     2461 atatgccaag ggactttttt tatttcctat cctatcctga agaaataatt aattacacat
     2521 gctagcaggt tcctgctttc ataaaaaaac tacacggagg tcagtaaaat gaatattatt
     2581 gatgaattaa cttggcgcga cgccattaat cagcaaacaa acgaagaaag acttcgcgag
     2641 ctcgttgaag aaaagagcat ttcactttat tgcggcgttg atccaactgg tgattcgatg
     2701 catatcggac atttgattcc atttatgatg atgaaaagat tccaattggc aggccatcat
     2761 ccatatattc tgattggcgg cggaacagga acgatcggcg acccaagcgg ccgcaaaaca
     2821 gaacgtgtgt tgcaaacgat ggaacaagtg caacataacg tggacgcact ttccaatcaa
     2881 atgagaaaat tgtttggtaa agatgcaaat atcacatttg tgaacaacta cgactggttg
     2941 tcaaaaatct ctttacttga atttttgaga gactacggta aaaacttcaa catcaacacg
     3001 atgttagcaa aagatatcgt tgccagccgt ttggaagtag gaatctcatt tacagagttt
     3061 acttaccaaa tcctgcaatc tatcgacttt ttgcatctgc ataaaacata cgatgtgcaa
     3121 ttgcaaatcg gtggagcaga ccaatgggga aatatcacag ctggattaga cctaatccga
     3181 aaattagaag gaccagaagc agaagcgttc ggtttaacga tcccattgat gctgaaggct
     3241 gacggaacga aattcggaaa aactgcaggc ggtgccgttt ggcttgatcc gaagaaaact
     3301 tcaccatttg agttctacca attctggttg aatcaagatg atcgcgatgt agtgaaatac
     3361 ttgaaattct ttactttctt gtctcaagaa gaaatcgaag acttagcgaa aaaagtagag
     3421 acagaacctg agaaacgtga agcacaacgc cgtttggcag aagaagttac aagatttgtg
     3481 catagtgaag aagacttgaa agaagcgcaa aaaataacgc aagcattgtt ctctggaaat
     3541 attaaagaac taaatgcgga agaaatcgcg caaggattcg gtaaaatgcc gaacgttgaa
     3601 atttcaagca cacctgaaaa tatcgtggaa ctgcttgttt ccacaaaaat tgaaccatct
     3661 aaacgtcaag cacgtgaaga tgtttcaaac ggagctataa gtattaacgg tgaccgcgtt
     3721 accgatttaa attttgtcat aaatccatcc gatgaattcg atggtaagtt tgtggttatt
     3781 cgaaaaggta agaaaaatta ctttttggcc aaagtaattg attagaattt agtaagtttt
     3841 taccttgaaa aagggaaata ctattacaac attccaattt tgaaacccat cctaaaatag
     3901 aacgctggtt gtcgttaata caatctttgc gagggagttt acatttgtaa attccctcgt
     3961 tttttttaat tatttcaaaa gtgtgaacaa ttcggataat tgatacatat taaaatatga
     4021 ttaaaaatca ggccaaaact tagtttacag aatagaagaa caggtgtaat gtagggctcg
     4081 acaaaattta aatatttttt taggagggtt tcactatgag tgaatcattg tcgaaagatc
     4141 taaacctaaa tgccctattc attggggaca aagctgagaa cggccaaatc tataaagctt
     4201 tgttgaatga attggtggat gaacatttag gctggcgtca aaactacatg cctcaagaca
     4261 tgcctattat tacgccagaa gaaaaaagca gtgctagttt tgaacacact gtgaataaaa
     4321 caaaggatgt tctatcagaa atttcagcgc ggatgcgtac ccattccgtt ccttggcata
     4381 atgccggacg ttactggggt catatgaact ctgaaacatt gatgccatct ttactagcgt
     4441 acaattttgc tatgctatgg aatggtaata acgttgccta tgaatcttct cctgcaacaa
     4501 gccaaatgga agaagaagta ggaatggaat ttgcaaaatt aatgagctat aaagatggct
     4561 ggggccacat cgttgctgat ggttctttag caaacttaga aggactttgg tatgcccgca
     4621 acatcaaatc attgccgctt gcaatgaaag aagtgacacc tgaactagtt gctggaaaaa
     4681 gcgattggga attaatgaac ttgtcaacag aagaaatcat gaatctgtta gacagcgtcc
     4741 cagaaaaaat cgacgaaatc aaagctcact cagcacgtag cggaaaacat ttagaaaaat
     4801 taggaaaatg gttggttcca caaactaaac actattcatg gctaaaagct gccgacatca
     4861 tcggtatcgg tttagaccaa gtgattccag tgccagttga ccacaactat cgcatggaca
     4921 tcaacgaact tgaaaaaatc gttcgtggtc tagcagctga aaaaacacca atcctaggtg
     4981 tagtcggtgt tgtaggatca acagaagaag gagcaatcga cggtatcgat aaaatcgttg
     5041 cattgcgtcg cgtattagaa aaagacggta tttacttcta cttacacgta gatgctgctt
     5101 atggcggata cggccgtgcg attttcttag acgaagacaa caacttcatt ccatttgaag
     5161 atttaaaaga tgttcattac aaatacaatg tctttacaga aaacaaagat tatatcttag
     5221 aagaagtaca cagtgcatat aaagcaatcg aagaagcaga atctgtaacg attgacccac
     5281 ataaaatggg ctatgttcca tattctgccg gtggtatcgt catcaaagat atccgcatga
     5341 gagacgttat ctcttacttt gcgacatatg tatttgaaaa aggcgcagat atcccagcat
     5401 tacttggggc ttatatcttg gaaggttcaa aagcaggggc aacagcagcc agcgtttggg
     5461 cagcacacca tgtcttacct ttgaatgtca caggatatgg taaattaatg ggtgcatcaa
     5521 tcgaaggtgc acatcgtttc tacaacttct tgaatgactt gtcatttaaa gtaggagaca
     5581 aagaaatcga agttcatcca ttaacttatc cagatttcaa tatggtagac tatgtattca
     5641 aagaaaaagg caacgatgac ttagttgcaa tgaataaatt gaaccatgac gtatatgatt
     5701 attcttcata cgtgaaagga agcatttacg gtaatgagtt cttaacttca catactgact
     5761 ttgctatccc agactatggc aacagcccat tacaatttgt gaaccaacta ggattctcag
     5821 atgaagaatg gaaccgtgcc ggaaaagtaa ctgtattgcg tgccagcgta atgacaccat
     5881 acatgaacaa agaagaacac tttgaagaat atgctgaaaa aatcaaagca gctcttcaag
     5941 aaaaattgga aaaaatctac gcagaccaat tattggcaag cgaagcaaaa taagcaaggt
     6001 aatcatgtta gaaaacagct tgaatactaa ctgtttctga cgcatgtttg gatctgaaga
     6061 gcaaggggag acctaagctc ttcgatccct ttgttttgag gttgtctttg aagaaaccct
     6121 tcaagcaata aactcaaaga atcccctcaa atattcgctg aattgtttcg tttattgctg
     6181 aaactacttc aaagacaccg ttattttatc caaaaaatca aggagagtgt caacggtgag
     6241 taaatctgaa gtaagtaaaa tgggtcttgg gacctttatc ggtctgacaa tggcactgtg
     6301 tgcaacggtt cggagtattc ctacgttagc cgcagtcggt tggacattga ttttttattc
     6361 aatttttgca gttttgtttt ttgccggtcc gatatcaatg gtttccggtg agctttccac
     6421 gatgcttcct caagaaggcg gtcctcagtt atgggtaaaa acagcactgg gcagcaaatg
     6481 gggatttgtc gttgcatggc tattatgggt gcaaatgttc ccaggtatgg taatggtcgc
     6541 ttctacttta ggaccattgt taggaaacac atttggtaat gtagaacttg gcaataatca
     6601 ctggttcgtt ttaggttgta ttttagttat ctactggatc atcacgatct tgaacttgaa
     6661 attcgatatg gcaaaagtcg gcgggaacat cggtgtatgg ctaggggtat atatcccagt
     6721 agtcatcatg ttcgttttag gtgttttggc agccttcaaa gtcggactag tctcaaacgg
     6781 ctatctaggt gacttttcat ggtcaaaagc tttccctgac ttggaacata ttgattcatt
     6841 gaaatactta gctggtatca catttatttt tgttgggatc gaaatgagtt ctgtttacat
     6901 gcctcgtttg aaagacgcaa caaaaaatta tacaaaaggc gtatttatcg ctttgatcgg
     6961 attagtttta ttgaatgtca tcaatgcgat gcttgtagca aatgtcgtac cagatggaaa
     7021 aatggaatta gcaaatatca cgcagcctat cttgattgac tgtcaaatcc taggcttacc
     7081 agaagtgatc ggaaatatct tcagcttcat ggtatttatt ggtgtgcttt tacaattatc
     7141 agcttgggtt actggaccat caaaaacaat catccaagta gctcgtgaag gattccttcc
     7201 accaaaattc ggattccata aagaaaacaa atacggcgta tctagaaatg tcgttctaac
     7261 acaatcaatc gttatttcat tatttgcttt gctttatggt gtaatggatg acgtaagtgc
     7321 tgtattcttg acacttacaa atgctacaac tgttatttat tgtatcgtgt acattttgat
     7381 tgctgtttca ttgcttaaaa tgcgtaagaa acatccagaa ttcgaacgtc cttaccgaat
     7441 tggtaaaaac ggaaacggat tagcttgggt agtcagctgc atgctgatct tctctatcat
     7501 cgttgtcgta tttgctacat taggtacagc gacattgagt gatgcattat tagtagcagc
     7561 tatcacagtg gtcatgttcg ttattccatt gatcatcaat cacttcaaga aagattcttg
     7621 ggggattgag gtagaaaaaa gtttagaaga aaaataaaaa aataagtctt tcagccgacg
     7681 ttttgtcgct gatttagtag gaggtcctgc caagtgaata gttttttcat ggcagggcct
     7741 tttcatttga aataggagat cgacgatgaa taaaaatatt tctttaaaag aaagtatcgc
     7801 tgtattgatc gtgttgttgg cactcctagg agtactgatc atcggattcc agctctcgcc
     7861 acatatcccg atccttgcag ctttcatgtg tttgttgttt tatggcagat ttaaaaagtt
     7921 cacatgggat gagatccatg acgggatcat caaaggaatc actccaggga tcattccaat
     7981 cctgattttt cttttgattg gtgtcttagt tgcttcatgg attttatctg gaaccatccc
     8041 tacgattatg gtttatggat ttaaacttgt ttctgtgcgt ttctttttac cgacagcatt
     8101 tcttgtttgt gcaattgtcg gagtgactgt cggcagttca tttacaacga tttcgacgat
     8161 tggaattgct tttttgggga tcggtcattt acttggattc aatgatgcaa tgactacagg
     8221 cgcagtcgtt tccggtgcct ttttagggaa taactgctcg cctttatcag atacgaccaa
     8281 cttggcagcg ggtattggag gagttaatct attcgaacat atcgctggta tgcgttatac
     8341 ggatttccca gcattcgtcc tttcgttgat tttttatacg atattaggac ataacagtca
     8401 ttcagccgat ctagctagta tcaatgaaat gatccaaaac atgaaagcga gcttttggat
     8461 ctctccttgg acattgattc cactagttat tttatttggg ggggcaatca aaaaaattcc
     8521 ggctatccct acattactag cgggatcgac agctgctctc gttctttctt ttatccatga
     8581 gaaaagtttg acggtcggaa aagcagcaga catcttgatg aatggttatg tcgcaaatac
     8641 acctgatcca aagctagatg aactgctttc tcgaggcggg atcatgagta tgttaggttc
     8701 tgctagtttg attttacttg cattggcatt aggtgggtta ctgatcaaat atctgatcgt
     8761 tgataccatc gttcaagaat taaaagaaaa gatggaccga ccttctcgtt tagtcgggtt
     8821 tactgccttg agctgtatcg gaatcaatct gatcgttgga gaacagtatc tgtccatcat
     8881 tcttccagga gaaacattca agcgttcttt tgatgacgtt ggcttatcaa aaacctattt
     8941 gacacgaaca ctagcggatt caggtgcgac cgtcaattct ctcgtaccgt ggggagtcag
     9001 cggcacattc attatgggaa cgatgaaagt tagcgcgttg cattacttgc cattcgcctt
     9061 tttctcgatt cttgcacctg tctttacaat attaggcggt tttttattga atcgtcgacg
     9121 tgtaaaaaat tgaaggaagg aaaaaagagg ctgggacaga agcgtttaac tccaagaaat
     9181 aagaagaaac tcaagaaaat tgctttttaa atttttgtga atttcagctt atttccgaag
     9241 gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
     9301 cttttgtccc acgctctttt ttacagataa acaatgtgtt agaagtagta tcttctttta
     9361 cataaaaagc tgaccatagg ataaatggtc agcgtattta gtgagtcagc tatttagtat
     9421 gggttttcta aaggaacgac tgtctctctt ttgatcaagc gatgagggat gaccaaacgt
     9481 acgccactgc tcaattcttc atggatcaat tcgcgcagct tttgcatagc gacacgtcct
     9541 aattcggaga tatcgatatc gatacttgta aggtaagggt gtgttaatgt tgagaagatc
     9601 gagttgttga agctaatgac ggagagtgtc tctggaatga catagccata catttgcgcc
     9661 aactgcatcg tgcgaagggc gaagatatcg tcaatgacca ccaatgctgt ggcttcgttt
     9721 tctttcaacg ttttctcaaa ggacagataa tcctccggat gctctagtac tacactggat
     9781 aacgcaggaa gaccagccat catcattgct ttttgataac cgaaatatcg ttcgaaatac
     9841 aaactttcat gagtcgtgtt tgtcacaaac aggatattct tatgtccatt ctggatcaaa
     9901 aattctgttg cttgtttacc tagaagctgg ttatcattat ctacataaac gatttcgttc
     9961 tcatgatgat aaggctgtcc gatcaaagtg aaagggatgt ggttttggta taaataatca
    10021 atgacagtat cttgactatc tgaatacact agaataaaac catctacttg tttttgtaaa
    10081 tgcatacgct ggacgttctc taacagcaat tcaaaatttt tagcagtagc gattgctgta
    10141 gtcatatcat aattccgcgc ttcttcgttg attgcttcca tgatctcaag ataaaatggg
    10201 ttaccgatcc gctcttttga atcaagaggg ggtaagatta cgccgatggc actggaaatg
    10261 cgttttccta gattgcgggc agtcatgtta ggaacataac ctagttcatc cataattttt
    10321 cggactttct tctttgttgt ctcggaaata ctaggatgat cgttgatgac acgagagacg
    10381 gtcgaggtgg caacaccggc ttttttcgcc acatctttta cggtaatagc catgataaaa
    10441 acacctttct cttgttattg tgcagagaac cgataaatcg tctgactgat ccgttcttga
    10501 ccaggatgga ggttgatcga tccaaatttg gggaaatgaa cgatatcagg gatttcttgc
    10561 ggttcgattg ctaatccgta gtttgaatgc atctgttttc cattgacaga gaaatcagct
    10621 tcgaatccag tcgtagaaaa taataccata cttttattag aagttgctat agtcatttgg
    10681 cgaccagaat gcttctcgaa taattgtaag gaagttttgg aaagcctagg atgatgcaaa
    10741 agaaatacat catctagtcc atctggataa catgcaagga tctcttgcag tgttggggat
    10801 tttctaaagt cgattggcag tttttctttt tttgtccaat ttccagtagg cagtttttct
    10861 tgatctaatt caagcacacc atctaaaaag accgttaact gatgttcata gatgtctctt
    10921 gttccatttc ctgaaaggtt gaagtaggca tggttggtcg gattgcagat ggtttcaaca
    10981 aggctactag cagaggttgt catctctaat tcgttttttg ttaaacggta agtgatcgta
    11041 gcagtgattg gtcctggata ggtagaaaat tcatctttta agtaaaagac cacacctatt
    11101 gatgatggag ttttgaaaac ttctacttcc caaaattgga aagaccagcc tttcgtgcca
    11161 ccgtggatat ggtgtgatcc gcagtttttc tccaatctat gctcacccca agaagcatcc
    11221 cggatccttc cggctacagg tccgacagtt gctccaaaaa agcttttgtc tgttagaaca
    11281 tcttcgaaac gatcgtaaga caataaaaca ttttctgatt ttccttcttt gtctggtgcg
    11341 aagatttggt gcaggcgggc accgtaattg agaagaacga cttctaagta gtcgttcttc
    11401 aatgtgattt tctttatttc agtctctgga actggtacga cggagatctc tggattatcc
    11461 atggtcatgc cacttttctt tcaagtaatc gatcgtaaga aaagtcgtat cagggacaag
    11521 cgggatctcc tctccaaggt cttcagcacg tcccacgcca attggttgag cgccacttga
    11581 gaggatggct tggataccgg cttgcgcgtc ttcgatcccg atacattctt ctgcttttag
    11641 tccgacagct tttgctgcaa ggaggaagat atctggcgca ggttttccgt ttgttgcttc
    11701 tgccgggtcg gcaatagcat caaagtaagg tgtaagaccc attttttcaa gtaggaatgg
    11761 cccgttttta cttgcagaag caagagcaat cttgattttt tcttctttta aagaatccag
    11821 taaaggcacg accccgggga agacatcttt tggttcgatc gtctggatca tctctaaata
    11881 ataatcgttt tttcttttcg ctaattctgc aaattcttcc tttgaaaaag agtgttcttt
    11941 tccgccataa gctaaaagca aagagagtga gtcttctcgg ctgacgcctt ttagctgttc
    12001 gttgaatgtg tggtcgatcg taatgccgat ttcctgacca agctttcgcc aagcacgata
    12061 atggaattca gcagtatcgg taatgacacc gtctaaatca aacaataccc ctttaaacat
    12121 ttgcttcctc ctttcttagc gttatttcta gacgatccgc taaagaataa gactgttcat
    12181 agacgtaaat ttctagagga gaaccttcta ataactcgaa agtaacttgt tcatctgtga
    12241 ctgagatgaa caataaacgt ccgcgataat tgatatggaa agaataagct ttccaagttt
    12301 ttggcagaaa tggtgcgaat ctcaactgat catgggcagt tttcatttga gcaaatcctt
    12361 ggacaatcga caaccagcta ccagtcatgg aagtgatgtg caagccgtct tctgtgtcgt
    12421 tattgtaatt atccaaatcc aagcgagctg ttcgctggta catttctaca gctttgcttt
    12481 ccattcctaa ttctgctgct aaaatcgaat ggatacttgg cgaaagggaa gactcatgga
    12541 cagtcattgg ttcatagaaa tcaaagtttt tttgcttttc ttcttttgta aaagtatcat
    12601 tgaagaaata aatcccctgt agtacatctg cctgcttgat gaagcaagat ctaaggattt
    12661 tgtcccaaga ccaatgctga ttcagaggaa gatcgcttgt gggaatatcg gatacaggca
    12721 tgagatcttt atctaagaat gtgtcatgct ggacaaagat tcctaattct tcgtcaactg
    12781 ggaagtacat gttctctgcg atatctttcc atttggctag ttcttcttcg ctgatagtga
    12841 tagcagcttc tttttgataa gttaaataat tttctcgcgt gtaacgcaag acccaagctg
    12901 cgatcgtatt tgtataccaa ttgttattga tattgttctc atattcgttt gggccggtca
    12961 caccgtggat catgtattta tttgctcgtt ttgaaaaatg gacacggtct gcccagaagc
    13021 gtgcgatttc tgctaaaact tccagtcctt catttttcaa atattccgta tcgccggtgt
    13081 aattcgtata attgtagatt gcataggcaa tcgcgccatt tcggtgaatc tcctcgaatg
    13141 tgatttccca ctcgttatgg cattcgacac cagtgaaggt gaccatagga taaagcgcgc
    13201 cttttaatcc ttgttgctga gcgttgtgga ttgcttgcgg cagttgatta tgccgatatt
    13261 tcagcagatt cttagttact tcaggtttgg ctaaagcaag ataaagaggg acagcatacg
    13321 cttctgtatc ccagtaagtg gctccgccgt atttttctcc tgtgaatcct ttggggccga
    13381 tattcaatcg ttcatcttcg ccgtagtaag tagagaatag ttggaacaaa ttgaatcgaa
    13441 taccttgttg tgcttcttcg tctccttcga tgaccacatc ggcaagcttc catcgttctg
    13501 cccaagccgc tgtttgctct tcttttgctt ggatataatt gtttgtctgg ttcgttaatt
    13561 ctgagacagc tttttctgct tgctgcgctt ctggaatatc tctactagta agaacgataa
    13621 tttctttttc cactgaccat gtctcacctt gagataattc tgcagacaag caaccatgta
    13681 atgctaaagt atcttcgtga tagtcaggga tgactgattt tccattaacg aaatggcgca
    13741 ttgctgcagt aacagtaaat tgttcaatcc caaagttgtt tggaatagtt ttagtcgtca
    13801 aatagccgat ttcttctata gttccacgat caatctcatt ccaaaaatgt tcatcatagt
    13861 tgctgtcttc gttttgaaca tttccatcca attttgatac gagtttgact gttccgctac
    13921 cttcaagcat ttctatagtt aaatagatat aagctgattc ttttttgaca atgctcagaa
    13981 aacgttcgaa ggaaatgcgt actttattag tagaagtagt gattgtgaat gtacgagata
    14041 atacaccatt tttcatatct aattcccagt aaaagtcttc tggttccaaa gaagccaagt
    14101 cgatcagctg atcgttgatc gaaatatcta tcgcgataaa gttcaaggcg ttgatcactt
    14161 ttccaaagta ttctggataa ccatttttcc accagccgac acgtgtctta tcgggatacc
    14221 agacacccgc caaataagta ccttgatggt ggtcccctga gtaagtttct tcaaagttcc
    14281 cgcgcatccc catataaccg tttccaatac ttgttaaaga ttcttgcagt cgaagatttt
    14341 ttttatctaa ttgtgtcgtg ctgattttcc atggatcaat agcaaatagt cgtttgatct
    14401 gtttcatttt tctcctccta atgagctgtt ttatacgttc ataataaacg caaccgattg
    14461 cacaagtcaa tagacaaaag aaaatatttc atagaaaaaa gcttaaaaaa ataaattttt
    14521 aggagaaaat tatagaaggg aacacacgta ttataggttt tatagaatgg tatagacaga
    14581 aaaataaaaa ataaagcatt tacaaaaaag aaaacgcacg ctataatacg tttaacgaaa
    14641 ccgattgcgt aaaggagcta aacggaatga aaaagatatt tagttttgag ttttggcaaa
    14701 aatttggaaa ggctttaatg gttgtggtag cagttatgcc ggcagctggg ttgatgatca
    14761 gtatagggaa atcaattccc ttgatcaatc ctgattggac gccgcttgtc acaacgggcg
    14821 gagtaattga aaatattggt tgggcaatta ttggtaatct tcatatttta tttgctttag
    14881 caataggagg aagttgggca aaagaaagag caggcggagc atttgcagct gggatctcat
    14941 ttattttaat caatcggatc actggggcta tctttggtgt cacaagtgac atgcttgcga
    15001 atgaagatgc atttacccac actctgttcg gtacgaagat catggttaaa ggatttttta
    15061 caagtgtatt ggaagcacca gctttgaata tgggagtatt cgtagggatc atcgctggat
    15121 ttgtcggggc aatggcttat aataagtact ataattatag aaaattgccg gatgctttgt
    15181 catttttcaa tggaaagcgc tttgttccgt ttgttgttat cttatggtca acaatcgtgg
    15241 cattgattct tgctgtggtg tggccaaacg tacaggctgg aatcaataat tttggtttat
    15301 ggattgcaga atcacaagaa agcgcaccga ttctagcacc gtttttattt ggaacattag
    15361 aacgtttact attgccattt ggattgcatc atatgctgac gattcctatc aactatacac
    15421 agcttggcgg atcgtatgag attttatcag gcgcacaggc ggggacacaa gtatttgggc
    15481 aagatccgtt atggttggct tgggccactg atttagtgaa tcttaaaggt gcaggggata
    15541 tgtcgcaata tgagtttgtg ttgcataact ggacgccggc tcgtttcaaa gtaggacaaa
    15601 tgattggttc ttcaggtatc ttgatgggaa tggcttttgc gatgtatcgt aatgtcgatc
    15661 ctgataagaa agcaagatat aaatcaatgt atttttcagc agcgttggct gtctttctaa
    15721 caggtgtaac agaaccattg gagttcatgt ttatgtttgc tgctgtgcca ttatatgtca
    15781 tttattcagt gatccaaggg gctgcatttg cgatggcaga tatcttgcca ttgcgggtcc
    15841 attcatttgg gaatatcgaa ttgctgactc ggacaccgtt agctatcaaa gcgggtctag
    15901 gtggagatct tatcaacttc gtgttgtgtg tgatcgcttt tggtgttgtt acgtatttct
    15961 tagcaaattt cttgatcaaa aaattcaact ttgctacacc gggacgtaac ggaaattacg
    16021 acaatgacag tgaagaaacc gttgtatcta acagcggaac aggaacagca gatcagcaag
    16081 tggtccagat cattcatttg ctgggaggaa aagaaaatat caaagatgtc gatgcttgta
    16141 tgactcggtt acgcgtaagt gtaaatgatc gagaaaaagt agggactgaa gaagcctgga
    16201 aacgagcagg ggcgatggga ttgatcgtca aagacaatgg agtccaagca gtttatggac
    16261 caaaagcaga tgtcttgaaa tcagatatcg aagatctgct gcaatcagga gcagagattc
    16321 cagagccaga aatggtttct acaggaaatg gacaagcagg tagtaagcaa tacttaggta
    16381 tagaacaaga actagtttcc gcagcttctg gagaagtgat tcctttatct gaagtaaaag
    16441 atccggtatt ctcacaaaaa atgatgggtg acggctttgc tgttattcca acatcaagag
    16501 aagttgttgc gccgatcgct ggaaaagtga caagtatttt ccctagcaaa catgcaattg
    16561 ggctggaaac gaaagatggt atcgaagttt tgatccatat gggaatcgat acagtccaaa
    16621 tgaatcagcc agcttttgag attgctgtca aagaaggcca agaagtagga acaggaacga
    16681 aattagctga gatggatctt gatgtgatcc aaaacgaaga aaaagatcca acgatcatga
    16741 tcgtatttac aaacgataaa gtagaagaag tagtgatcaa gcaattaggt acagtgacag
    16801 ctggtaaagt gatcggctcc attaaaatgt aaggagaggt tgtgaaaaag ctgtcctgca
    16861 tcaagtcata agaacagcca aacaaaaata gctcctccta tttttcgttt ggttgggctt
    16921 attaccgaga agcagcagtt tgaatactgt ttagtggaga ggtcgtgaaa aagctgccct
    16981 gcaccaagtg ataaaaacag ccaaacaaaa aatagctcct ggtccattga gcttattact
    17041 gaggagttag caggagaaca ctatttgctg gagaggttgt gacaaaaatt ttgtcacaac
    17101 ccttttgttt cgaaatgaag acaaggagaa aaccaatgaa attcatgaca ctcaacacgc
    17161 atagctggct tgagccagaa ccagagaaaa aattgcaaga attagcagat aagatcctgt
    17221 tagaagatta tgaagtgatt gctttacaag aaatcaacca attactggaa tctgaagaag
    17281 tagaacctgc gaagctaatg aagttctgtc cggtaaagaa tcaagtacct atccgtaaag
    17341 ataattttgc ctaccgttta gttcagcttt taaaggaaca cggaaaagag tatttttgga
    17401 gttgggaaat gagccacatc ggttatgata aatatgaaga aggaaatgca ctgcttacga
    17461 aaaaaacatt ggaaagtcag gtccttaccg tctcccaatt tcaaaagaaa gaagattatc
    17521 agacacgcaa gatcttgatt gggaaaacaa agatagatga tcaggacatt tttgtctcga
    17581 gctgccactt ttcttggtgg acagataaaa aaagtggctt ttattttgag tggaaaaact
    17641 tggaaaacta ctttttggaa actcgcgtac cacttttttt cctaggtgat ttcaataatc
    17701 cagtagactc tcaaggttat tatactgtaa gagaaagttg ccttcttctt caagattcgt
    17761 atgttgtcgc gaatgaaaaa ggaaaagcag ctactgtgga aaagaaaata gatggttggg
    17821 aacaaaatac agaaaaattg cgtatagatt ttattttcgt ccctgaagga atgcaggtta
    17881 aaaaatatca acggattttt gatgggatcg atagtccaat catcagtgat cactacgggg
    17941 tggaaatcga attagatgtg aacgaataaa aaatataaca tataacataa aaaaactgat
    18001 taaaataaaa ctctccaaac gattataccg tttggagagt tttttgatcg gatgtattta
    18061 tcccttttgc ctaagcatgt aaaagcttgt atctatgata aataaataaa catagcaagg
    18121 taaaccagcc aatcaaagta agagaaagaa tgaaatagaa atagattggc gtttgataat
    18181 gaagcgtgag ctcgttgatt cctttttgct gcttcacgat tggggtcccg atatctgtta
    18241 cttcataatc agtcaatgtt tttccatttt gctgtagcac agtccgatcg tagacgataa
    18301 cagggatatg aacagttttg tccccagtgt ttttccactt gattgtcaaa agaccgcctg
    18361 gatgtgtttt tataaaagtt gtttcgtttt ctagtatttc ttcgttatac cggtcgtatt
    18421 tattgtcttt tgtctcctta tagattggca gatagtcagg tgtggttttt tggaaaacga
    18481 acaagctggt agacatatct ttgctgaaga atgttttcct tgcttcttcc ggcgtatcga
    18541 atagataagt atgtcgactg acaaatgttt cattttccca cttttccaga tgctggtata
    18601 agttctggat cgtctgtgct gaaaaaataa gaatgccaaa tatacttaaa agacggaacc
    18661 acttttgttc agaccactgg ttcagtgaaa gggcaaggta aagtagaagc aatagcgtaa
    18721 atggcacgaa aaatcgaaat ggaaactgga tcaaactaat aaaaggcaca tttttttcga
    18781 tcaatgtcgt ccatggaatc aagctagtag aaagaaaaaa gaaaacaaga cttgtgtaca
    18841 acacgatttt ttggaataag gtgttctttt gttgaaagaa aataaaaagc aaaaaacctg
    18901 ataatatcaa tataggaaag gccttagggt aatggagcca ataactgcca tccagatcta
    18961 tcgccttttg actcatttct ctgttaacaa agggaggaag aatcttattc ccactgtaaa
    19021 caatagctaa tcctgcccaa atatttgctg tcagaacaaa ataaagcaag accgacttaa
    19081 tcagttgaag cagtgctttt cctttttcag tctggttaaa aaagtaataa agataaaaag
    19141 ggatatacat caaagctaac atgatcgaag acagtaaatg gatctgtgtc ataagtccca
    19201 ctgagacagc gactttgata acagggaact ttttatgaag gacgaaatca gcaataggga
    19261 tcatacataa aggataaaaa caagtccccc agctagtgaa tccttggttg atagaccagt
    19321 attgaataga atacgtactc atgaataaaa cggctactgg tacacttacc caatttcgaa
    19381 cattgacata tctcagtaaa atcagcatag aaaatcctga aataagataa agaatgaaat
    19441 ttgaaagaat ctgataatga aaccagctac ctgcaagcaa cacgatcaat ccttgtaaat
    19501 aagcgaaaaa tgggccgtaa acggcattca cgatcctgcc actttgctgg aatccataaa
    19561 gggaaggaaa ataatggaaa tccccatatt taatttgttg cgcggcatca tagaaccgat
    19621 tgtaatggaa tggggcatca gcacccaaaa ttagtttatg tgtgaataaa tgaggcatga
    19681 gaaatgccaa agcaatgaaa aaaattgcta aaaatgggcc atattttttc aattgtcgaa
    19741 ctcctttgca aactttagat aaattcatca tatatcctat gcgagaggta ggcaagaaat
    19801 tagaaaattc atgaaaatat ttatgaattt tacaagaaaa acataagtga aagttctaag
    19861 gagagaggat gcgacataag ttttgttgca tctccttgtt ttgaaacaga atttttctta
    19921 atactcgaaa caaagtagct ttttcatgac ctctcggtaa acggtgttca atcagctgct
    19981 tctcggtaat aagctcacta gcctagaaaa tatgagaagc tattttctga ctagcgctct
    20041 tattactcaa agcataacgc agttttcaca acctcttcct taaacggtgt tcaaaagctt
    20101 gcatctgcgg taataagccc aacccaacaa aaaatatgag tagctatttt tttgggctgt
    20161 acttattact tgatgcagga cagctttttc acaacctcat cggctttcat tttctatttt
    20221 tttcctgaac ccatataata aaagaaagaa caaaaaaact tgcaagggag gacacatgtg
    20281 aagcagagta agcaaaaaca gtctcgtcga cttttgtttc gtttgttttt attaatatgt
    20341 atcgtcgtag ggttactgat cgctttatat ccattttatg tggattcttt aaatagtttt
    20401 attgatcaaa aacgtatcga acaagtacaa aaacggaccg cagctgaaat tgaagtgcag
    20461 cgaaagaaaa tggaggagaa aaaccaacga ttaactgacc aaggattcaa tcctggtact
    20521 gatcctttca atgaaaagaa cagaaatgaa agtacaacat ccagccaatt agaggaatgg
    20581 ctgatcggaa gtatcaatat acctaagatc caattaaata tttcacttta tgatcggctc
    20641 aatggtagga tccttgaaaa tggagcaggc gtcttgcaag gaacctcttt cccgcttggt
    20701 ggaaattcga cgcattcagt gatctctgct cacagcggtt tacctaatcg acggttattt
    20761 accgaattag atcgtttaga aaacggagac acgtttattt tgactgtttt gggggagaaa
    20821 ttggcttatc aagttaaaaa tatacaagtg gtactgccag atgacacgtc tgttctaacg
    20881 atttcagaag gagaagactt agttaccttg cttacatgta cgccgtatat gatcaacacg
    20941 catcgtttgc tagtgacagg acatcggatt ccttataacg aatcggtaaa aaaagaagaa
    21001 ggaaaaggaa atcaggaaag aaccatgcgg caatggttta ttcttgcagg aacgataata
    21061 gctgtagtca tactgttagt gtttatcgga cgactaatct atcagtatag attaagtaaa
    21121 aaagtgcttg atttttcatt tattattagt gattcgtcag ggaaaccggt aaatggtagt
    21181 tcattcattt tgaaacataa gaaaaaaaca cttactagaa atggtgtgcc gttttcagta
    21241 caaagcgacc atcatgggaa agtcaagctg gatcaattgc ctggtggaac ttatcgaatc
    21301 gtttcagctg atgatcctaa agttgctgtt tcttttggta ttagaaaagt aaaacaagag
    21361 aagatatact tttttgaggg gcgaaaactt gtaaaagagc ttcaaaaaaa tggtttttat
    21421 tttaaaatca atgaataagt aaggagagaa aaaatgatcc gcacaacaac aaaattgtct
    21481 gccaaagaac tggaatctgt tctatcgatt tggctgcatg ctaacttgga ttctcatgaa
    21541 ttcattccat caacttattg ggagaatcaa gtagaatctg ttaggaaagc tttaccagat
    21601 gcagtgattt acttagcaga aatagatcaa gaaattattg gttttgctgg ggtgattgat
    21661 cagtttatcg caggaatctt tgtgaaaagg gaataccggc aacaaagaat cggtacgcaa
    21721 ctaatgaaaa aaataaaaga aaccaataaa acactgactt tgacagtcta tcaaaaaaat
    21781 caatcagcta ttcatttcta tctttcacaa ggttttacaa ttcaaacaca gcaaaaagat
    21841 gaagcagtaa atgaggagga acttgtgatg aaatggaatc aaaacgctga tttttgatga
    21901 acagaataac gaaataatgc tcccagccta attttttgct gggagcatta tttcattttg
    21961 aagtcactaa tttaactgtg tgaatatgca gttgttacaa tctttgataa acggtgttca
    22021 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccat
    22081 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttgata aacggtgttc
    22141 aaacggctgc ttctcggtaa taagcccact agcctagaaa atatgaaaag ctattttcta
    22201 actagcgttc ttattactca aagcataacg ctgatttcac aacctcttga taaacggtgt
    22261 tcaatcagct gaccctcggt attagctcaa aaaacggcaa aacatgagga actatttttc
    22321 cgtttctttc tctaatactc aggtcagaac gctgatttca caacctctct ttcacaacct
    22381 tattttactc gtttataatt ttcatcgaag tattttccgc tacggatatc atctggcatt
    22441 ccatcgtatg gttcttcgct aataacatct tcattgtcta tacctgcatc acccatataa
    22501 gtgatcgtag caaattcagg aacaagtaat tttggataag tttcatattt ttcacgagct
    22561 gcttccattt ctgcgatatg ttcgttttgg aaagtaacag tgatttctgg atgttcttca
    22621 acccatttag ggaaaaagat cattccgtga agtttctttt catttggcat aaagtccaat
    22681 gccacgtctg tccctgttgg ctctgtccaa gtgattttga atacgccgtc cgtcaattta
    22741 acgatatcag cttcttggtc ttttacccaa cggcctttga ccattccgcc atggatacgg
    22801 taatcaacag tatggtcatt tttggcatac cattcatatt cccaaccatt gtcatacgta
    22861 tagataaagt gagttccaat aaaatcttcc aattctttaa attctttcat gtttaagcat
    22921 ctccttataa attaaaacat gtaataaact acatgttttc gattactcat ttatgataga
    22981 atggatttaa agatttgtca aggagggaaa actgatggca agaaaaaata cacttcgata
    23041 tattttgtta gggttattaa gtaagaagaa gatgacagga tatgaattga atcaatcgtt
    23101 caaaaatgaa ataggagaat tttggcaagc aaaacatagt cagatttatc ctgaacttgc
    23161 aaaaatggaa gagcaaggaa tcattcagca tcaagtagag atcacaggtg aaaaattgga
    23221 aaagaaagtt tatgaattaa cagaagaagg gaaggaacaa ttggcagagt ggattcatac
    23281 acccaccaat gaactaccgg ttaatcgaga cgaatttgtt ctgaaattgt atttcgtcaa
    23341 agacgtgaat gatccggcat tagtagaaat tattcagcag cagcgtgacc ttcatgaaga
    23401 aaaacgactt catctattag agcgaaaaaa aacgatcttt cctactgaaa aagaaaaaca
    23461 tgaaaattac ggacactacc tgattttagc gcatgcgatc aatcgggaaa cagaatatgc
    23521 aaattggcta acagatgtat tagaagaaag taaccgaaaa aaataaaaaa atatccgaac
    23581 agtgatggag aattcctttc tatatgaaag aaacgcagat caccgttcgg atattcttat
    23641 gttcaaacag gccttttttc aagtatttat gtagcatcta cttattaaaa agcttggtac
    23701 ttaggagatt agtggcgttt agagttcttt taacaactgt tcgtagtcag ctttttcatc
    23761 gacaggaagg agcttgtaag ttaattcgtc tgtctcgttg tcatgaatga tgtacatctt
    23821 agcattctct tctttgaagt taccgatttg taaaatcgta tagcgttcgt cgatcaattc
    23881 gatatctttg attgcttttg aaaaaacttt acggacatgg tgcattttct tttggcggat
    23941 ttctgccttt tctgcagctt ttgacaaagt aaatccgtca tctaaatatt ttttgatcag
    24001 ttctactttt atgactgtag gtaaacgata ttttctagga caattcgcat cagcttcgat
    24061 cgaacgaatg aatccctttt gttcccaata ccttaattga cgtggagaca cacctacgat
    24121 atcacttaat tcactgatgc caacaaccaa ttgatcattt tccaacaaac gtttaaggga
    24181 agccccaacc atttttgtca ctccttttgt cctcttgctt cccttaatta tatccgtatt
    24241 aacttatgtg tcaagtaggt tgacaaatag cgaatttagg actaaagtat tctagtagtt
    24301 tcagaaaaaa ttcagaaaga aggagaaata tgagtaacaa ccaacccgta gatatttacg
    24361 gaaaaccata taatcggaca cttttagttg ttgtcttatt aattggtaca ttttgtaccg
    24421 ttttgaatca gaccttattg acgacagctt ttcctacatt gatgaaagct ttcgatattt
    24481 cagcgtctga tgtgcaatgg ttaacgactg gtttcttgct agtcaacggg atcatgatcc
    24541 caataacagc atggctgatc aataaattca gttcaagaaa tttatatctt tctgccatga
    24601 cgatcttttt gatcggaacg attacttgct ttacagcacc taattttagt acattactaa
    24661 tcggtcgttt gatccaagct tgcggtgtgg gagtatcgat gccattattg caaaatatca
    24721 tgctatctat tttcccgcca gaaaaaagag gatcagcaat ggggatgtca gggatcgtta
    24781 ttggacttgc tcctgcttta ggccctacat tatctggtta tatcattgat aactaccatt
    24841 ggcgtgactt gtttggtatg gtactgccaa tcgttgttct tgtcttggtt ttagcattct
    24901 tcctaatgaa aagtgttatt ccattgtcaa atcctagcat tgatatttta tccgctattt
    24961 tatcaacgat tggattcggt agtttacttt acggattttc tagtgtagga gatgacggct
    25021 ggggaagctt gaaagtaatt ggtttcttag ttgttggtgt gattttcatc gccttgttct
    25081 catggcgtca attacatttg gaacatccat tcttggaact tcgtgtattc aaatcatcta
    25141 cattcacgat tgcagctgtt ttagctggtg taacaaatat ggcgatggta ggggcagaaa
    25201 tggtcgtacc attgtatatt caaaatattc gtggagaatc agcattccat tctggcttga
    25261 tgttgttgcc gggtgcattg atcatggggg ctatgatgcc tgtgacgggt gcgattttcg
    25321 ataaatacgg cgcgaaacgt ttagccatca cagggatgtt cattttgaca gcggcaactg
    25381 caccatttgc tttcttgaca aaaacgactc cagttttata tatcgtgatc ctttatgcga
    25441 tccgtatgtt tgggatctcc atggtcatga tgccggtaac gacttctggt atgaatgcat
    25501 tgccaaataa cttgatcagt catgggacag cggtaaataa tacattccgt caaatcgcaa
    25561 gctcgattgg tacagctatt ttgatcagtg tgttgacgaa tgtgacaaaa gcaaatatcc
    25621 caagcaaatc tgtattggat aatacgccat tagcttataa agatcaggca atcaatgcca
    25681 cactgtcagg ttaccatgcc gcgtttatcg tagcgatcat ctttgggatt cttggcttta
    25741 ttattgcctt tttattgaag aaaaaagaac ctgttctaat gaaagcgggg gataatgcat
    25801 gattattgga gttttgattg tcagcttgct tgcttttgct ttaacgaacg tgttcgctaa
    25861 aaaaacatgg caaacctttt tatctttgat tttcggacta atcttcgtcg cttcactaag
    25921 tttgatcgtc gctaatttgt ccaatcactt tggaatggaa aaagtaacgg aaacaaaaac
    25981 agaaaaaatt gtttccagtg ctgattccca aggagcagat atgcttttgt ataaagcttt
    26041 agggaatgga aaagaaaaag tgtatcttta ccgaacaaat gaaaaacaag aaaaaccaaa
    26101 agcaactggt acggataacg aaacaaataa agtagaaaag acagacggcg atgctgaaaa
    26161 agtgacgaaa actacttatt gggaatacaa aaacgacatg tataaattct ggttcaatat
    26221 tgctgataac aatcacgaat atgacagccg agtgaataca ttcaaaatcc cagagacatg
    26281 ggtagaatta agtacggacc aagcagccaa gttagctgaa ttagtgaaga aacagcaaag
    26341 tacgatggaa agtgaagcaa aagcttatgt tcaagacggt atggtaaaag caatgactga
    26401 aaatccaaaa atgagcaaag cagagcaaga acaacgtacg aaagccttag ctgctgaatt
    26461 ccaacaacaa gcattcgcaa aattagtgaa agaagcaaaa ggcgaataat attttgaaca
    26521 ccatgtagga ggttgtgaaa aagctgctct gcatcaagca gtaagaacaa tcagataaaa
    26581 atagcactcc atatttttca tctgattggg cttagtgccg tagatgcaag cttttgaaca
    26641 ccgtttaagg aagaggttgt gagcctgccg tttaactctg agtcacaagg agcaatttca
    26701 aaaaacagct tctcatgttt gttgactatt gtgacttgtg accgaggagt tggcaggaga
    26761 acaccgttta ttcgaaaaaa ggaactgcga caagactttt gtcacagttc cttctttttt
    26821 ttgctatgct atactagatg taaaaaatag gagaaataaa atgagggaaa tttattcttt
    26881 tgaatcaaaa atctatgctt ccgaagtcgg caagggcggg gcatatatta tttttccgta
    26941 tgatataaga aaagaattcg gtgctggaag agttaaagtc caagcaactt ttgacaatgt
    27001 gccgtatgaa ggcagtatcg tgaatatggg cgtgaaaaat ccagatggtt cagtctgcta
    27061 catccttgga ctacgaaaag atattcgtaa aaacatcgga aaagaaatcg gtgatattgt
    27121 agcagttaca gtgaagcaaa aataaaagag gttgtgacaa aagacgtgtc acaacctctt
    27181 gataaacggt gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc aaaaatgtgg
    27241 aaacccatgt ttacaaaatt tttcttacta ctcgaaacga agcggcttta tcacaacctc
    27301 ttgataaacg gcgttcaatc agctgtccct cggtattagc tcaaaaacgg caaaatatga
    27361 gaagctattt ttccattttt ttctctcata ctcaggtcat aacgctgatt tcacaacctc
    27421 ttctttcata atggtttttc accatcttta taataaactg gttctttgat atggaaatct
    27481 attttttcca accaatctaa aatcttgtaa cctaactctt ctgacatcgg aaaagaggcg
    27541 ttgatttgtg cattggagat atggtcaaca tggataccgg aagtgatcgt acaacttccc
    27601 ggaacggcag ttttgatgat cacagctatt ttttcggcta agacatcgtc tttatggaac
    27661 cgaccatcgt ggctagggaa acgtacagtt tgcatctctg tatcacgaga aaatgtagtg
    27721 actgttccga tatgtggtgt atctccgcct gttaaagtga tcaataagtc tttaccgatt
    27781 actttaacag cagcttggat cgtgaaacct agtttggtta cttcaactat cgtttctttt
    27841 tccatattaa tcaccctgcc aacgttttga atgctcattt acaatttcaa attgatccaa
    27901 aagtttcatt gtatggtgat taacaagatc ttggatcgtt tgaggctgat tataaaaagc
    27961 aggaattggt ggtatgattt gcacacctag tcgtgctaat ttagtcaagt tttccaagtg
    28021 aatggcattt aatggcgttt cacgaggaac aatgactaac tttcgttgct ccttcattgt
    28081 tacatcagca gcacgtgcaa tcaggttgtc gcctaaacca acagagatac ctgcgactgt
    28141 tttcatactt gccggtacga tgatcattcc atcggtcaaa aatgatccgc tggcgacagc
    28201 agcacctaaa tctttgtttg aataatggta atcaaacagg ctttcgaatt cagttagtga
    28261 taagtcggat tccagcttta agttctgttt tgcccatgga ctgatgattc cgtgtgtttc
    28321 tatcatcgga tattctttta gtttcttcac taggttgatg gcataaatgg tgcctgaagc
    28381 gccacttaca ccaatcacga tttttttctt ccgttgtgcc aattgttcca ttttccgcac
    28441 tcctttttat gatgtcaaaa cagttgcaga tcattcatta tttcaaatat ttttcccaat
    28501 cttctacttc ttggaatttt gcacgtacaa aatgttcttt catatcgaaa ggaactgtac
    28561 cgtcaaagat agtttttgca cttatcccac ggaaacgaat agagccagga ttatattctg
    28621 gtaattctga tgggtccaat ggatggttcc tcattcctgg gatcacgatg atatctttat
    28681 ctccttggaa acgagtgttg aaagtccaaa ctacatcgtt catgtcgaaa atatcaacat
    28741 cttcatcgac taaaatgact gtcttcaatt ctttgaaggc tgataaagca agaatagctg
    28801 cttgacgctg aataccttca tccgcttcgt tttctttacg gatttgcatg atggtcatca
    28861 atttaccacc acctgcagga ggattgtaga catttactac ttttcctgga atagctttat
    28921 cgaccaaggc aagaatactt gcttctgtag gaataccggc catagatacg tgttcttcac
    28981 tgggaccaat cgttgtttgc atgattggat tgtcttttcg gtgagtaact gctttgactt
    29041 tcactacttg tacagctgga ttagcatccc cagtatagcc aggaaattct ggcattgctt
    29101 ttcctgagtt cgtgttgata tcttcttgca ttgtttcatt tggcatgatt tctgcttcaa
    29161 tgatgaattc agcacgagca atacccactt catcgactgc tataccatca acgatttgaa
    29221 taggttcgtt acgcaaagct cctgcacacc atagttcatt gtagccaagt ggagtggtag
    29281 gtggttcgaa agtcgtgcca atggcaattg ctggatctaa accaatattg atagtgatag
    29341 gcattggttt attcaaggct tcgaattgtt tttggaagtg accaatgtgg cgtccgccag
    29401 gcatgatgta cataccgatc gtatctttat cttctagaac catacgatgg atcgttacat
    29461 cgctcattgt tttttctgga tctgatccta agactacacc cattgtgatg tatggaccag
    29521 catcttcagg agtatttgta ggtgcaggca aaatgttgcg aatatcgaag tctgcatcgg
    29581 tagctagatg aacgacttct tgtgctggag cgtctgcttt gtctactttc acaggtttga
    29641 ttggattttc tacagcgtct ttcaagaaac gaccaagtgt tcgataatca tggtgcaaaa
    29701 tttcacctac acgtttacga ctagccattg ttccgatgtt cacacgtgtg ccggggaaac
    29761 ctttgatatt gttgaaagtc atagctggtc cttcttgagt cggacgtttt actgtaccgc
    29821 cagcaccgat ataacggtaa acaccagata attctgcttc aggatcgatt tcaatatctg
    29881 tttcgtgata ttgacctgga agtgttttta gttcttcgat gacttttctt aaatcatacg
    29941 gttgatctgt catgagagat tcctccttag ttgtctttac ctatctatag taaaaggtga
    30001 aaaagaggaa aacaattcaa cttgttcggt aaagtattct acaatggaat ggatgcgttt
    30061 ttttgataat agaataaact atgatatact tatcactaaa agaaagcgca tacttagtgt
    30121 tggaggaagg aaagtgaaaa aaatggagga gtcatttcct aaagcagtaa aagtggaaaa
    30181 tatagcgaat attttgaaag ttacatttga aaacggagaa gtaaaatacg tgaaatctca
    30241 ttggacagaa gagataaccg atgccttgca atttggaaaa aaaggaagag gaaaaagaaa
    30301 aaatcttctc gcactttctc gtaatatgtg gattgggact gaagtaacga ttgaagcaga
    30361 tggaacagta ttcataaatg gaaaagacag gtatacgcca gaagagttgt ggtacaaagg
    30421 aaagaaaagt ataccagaat tataaaaaat aattaaaaaa aaacagcgcg aaacaaggaa
    30481 catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat gactcaaatc
    30541 taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc ttttttcgct
    30601 gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac gatcactaga
    30661 ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat gttgtataag
    30721 atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat cccagaagaa
    30781 atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt acgtgcacct
    30841 aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata agtaatgata
    30901 ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag gacataagtg
    30961 atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc agtataaatg
    31021 attttatctt catctgattt gcctttattg taatcatcta gataatccaa gatcttttct
    31081 ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc tccacctaaa
    31141 taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa gacatttaca
    31201 ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac gatttgagtc
    31261 gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga agaagatttc
    31321 acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat cgcatcataa
    31381 tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt gttgtttact
    31441 aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc tttcacatca
    31501 aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac gatcagcacg
    31561 acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc ttttaaaaag
    31621 ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc tgtcattgct
    31681 gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga gaagctgaca
    31741 ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg ggtaaatccg
    31801 atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt ttgatcaatc
    31861 ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt tttgctgtct
    31921 ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt agtcgttacc
    31981 ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc gatttgtttt
    32041 tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat actggaagca
    32101 aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga aagctttaac
    32161 gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt tggtcgttcg
    32221 atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat tcgatctgcg
    32281 tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc tttcgataac
    32341 tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc ggttggttca
    32401 tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac tcgctgcatt
    32461 tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc gactcggtgc
    32521 aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa tgtcatccca
    32581 agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg aaaaatgaaa
    32641 ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt agtagacttt
    32701 ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac attcagcata
    32761 gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg tctgaaagcg
    32821 acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata tttttttata
    32881 tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta tactaataag
    32941 tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg aactacaaga
    33001 aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt ttagtagtag
    33061 aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg ggcttttcag
    33121 tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca cgtatagaat
    33181 taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta gactcattac
    33241 gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat tttgaggata
    33301 agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc gatgtcaatg
    33361 agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac agcagtagct
    33421 tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa gatcagcatt
    33481 cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct tatcccaaca
    33541 agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg aatacagaag
    33601 aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag acggaagatt
    33661 ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga tgaaaaaaaa
    33721 gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga tcttagtgac
    33781 actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct cgcctgctga
    33841 atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca gtatggtcgt
    33901 gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca gtgcgctagg
    33961 gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg aacagcagaa
    34021 agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag aactcaatag
    34081 tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca aaacaccact
    34141 agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg acgaagaacg
    34201 ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat taacagagaa
    34261 catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga aagagtatcg
    34321 attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg agaaagaaca
    34381 gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag agtttttgta
    34441 ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca atggacagat
    34501 tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg attcaggtgt
    34561 aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag gagatacaag
    34621 tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc tagaactgca
    34681 tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa tgatacggtt
    34741 aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt ttttaggggt
    34801 ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc ataaaaatcg
    34861 ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag gacatgatgc
    34921 aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga tggacagaaa
    34981 atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg aagccaaaga
    35041 atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag aaatagggag
    35101 aatcacagta ttttctttta atagattctt gactgttttt tccataacaa tagcaatccc
    35161 catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat aagagatttt
    35221 tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt tcgtagtggg
    35281 gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct ttttactagc
    35341 taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag gcaaccactc
    35401 aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac tatgttcatt
    35461 ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg taaattcagg
    35521 atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca ttgtcggtat
    35581 cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca cttcttgcat
    35641 agcatggtat tgttctacga cattttttac atatgggagc aataagcttc cagcttcggt
    35701 caattcgatt tttctatggg cgcgttcgaa taatttgact tctaattctg tttctaacga
    35761 aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag ttttggtgaa
    35821 attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca tcatattcct
    35881 cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta atcttctctg
    35941 tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct gatttttttc
    36001 gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc aatgtaggtt
    36061 ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt gaacatttta
    36121 aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc tattccattg
    36181 cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca gcgattggtt
    36241 tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa gtacctattt
    36301 ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc agcacattag
    36361 tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt ttagattctg
    36421 ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt ttcacagcag
    36481 ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc caattcatta
    36541 ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca ccagacatga
    36601 tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt agaatgatca
    36661 acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc atgccactga
    36721 gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag agaagccatt
    36781 ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg acaaactgat
    36841 tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac acttgagaat
    36901 agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgctagc
    36961 agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct
    37021 ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag aaggatatct
    37081 tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat
    37141 gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag attctttaga
    37201 ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc
    37261 aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag
    37321 attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct
    37381 taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa
    37441 atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg
    37501 tcctcattcc actagcactg gggtttatat tggttattgg gggactattg gttgctttac
    37561 tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca
    37621 tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg
    37681 gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa
    37741 caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta
    37801 caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac
    37861 attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt
    37921 atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg
    37981 ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa
    38041 cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa
    38101 agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag
    38161 gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc
    38221 atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat
    38281 caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag
    38341 gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag gccaatgtgg
    38401 ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca
    38461 aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg gaaaacagtg
    38521 gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag ccaataacaa
    38581 atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca
    38641 atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt
    38701 taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata
    38761 actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa
    38821 ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac atacttatat
    38881 cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc
    38941 gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt
    39001 gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa
    39061 gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc
    39121 agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt
    39181 tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa gcggttggta
    39241 catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaaa
    39301 ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag tagcatgaga
    39361 gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt cgtattagct
    39421 ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg ggcacttttt
    39481 ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac tgtagtcagt
    39541 cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat tcgagaaaat
    39601 ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa agttcggaat
    39661 ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga aatctcagca
    39721 gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc gtattatcaa
    39781 gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca atacccgtat
    39841 gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt gtctgacgag
    39901 ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat cgaaacacgc
    39961 ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg tcaacaagca
    40021 aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat cactcaaatg
    40081 gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg gaaagtccaa
    40141 ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc tgttatcaat
    40201 acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag aaggagttta
    40261 ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga caaaattgaa
    40321 agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa tcagtatata
    40381 aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg ttttcatcgt
    40441 tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag aagaatacta
    40501 tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg gacaagaaac
    40561 agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa aagaactgga
    40621 cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa tcacctggaa
    40681 caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc cgaaaacagc
    40741 actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga agtacagaat
    40801 gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag cggcatcagc
    40861 ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc tggtaagtcg
    40921 acacttttga atattttatc cacattagat aaaccaacag atgggcatat ccgaatcaat
    40981 caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc gaatgagatt
    41041 ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga aaatatcgct
    41101 gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt ccaaaaaatt
    41161 gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat ttccggtgga
    41221 caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat tctattaggg
    41281 gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga tacgatggac
    41341 gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc gttttctgca
    41401 agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga agtgaaacgt
    41461 ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg aaacttggaa
    41521 cagtaggggg agattgacta tgctttggaa attatcactt actgggatca agagccgttt
    41581 aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt tttacatgtt
    41641 tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg cggctacagt
    41701 tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt atattttata
    41761 tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta tgatgctggg
    41821 agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag tagggatcgc
    41881 agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc accgattatt
    41941 agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc acgggttgtt
    42001 gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca atgctttttc
    42061 gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa caagaatcaa
    42121 gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc taggctgcgg
    42181 ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg tagccttagt
    42241 tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct ttttatcatt
    42301 actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat tgtcacagtt
    42361 aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc tgtttgcctt
    42421 ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag acttgacaga
    42481 aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc agcaaaaaat
    42541 caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg cgggaacgat
    42601 ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac atgaagatgg
    42661 aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg aactgactgt
    42721 acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa tcaaggtagt
    42781 cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc tagttcaggt
    42841 aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa ataaaatgaa
    42901 caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt atgaagtgta
    42961 taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag ctttcttgac
    43021 catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg acattgctcg
    43081 ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat cgatccgtag
    43141 agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg tcttattcgg
    43201 attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg ttccgtttac
    43261 gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat atactggaat
    43321 cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaagc
    43381 aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg
    43441 aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc tttttttcac
    43501 ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag ctgttaagaa
    43561 aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg caaggaacat
    43621 ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca cgaatcaaaa
    43681 tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc aaacaataat
    43741 cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat atctagaaaa
    43801 agacccttat atcggtgctt caatcggacg tatggctgga cggattacga atggcacatt
    43861 caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc attgtttaca
    43921 tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca atggtgaaaa
    43981 cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat ttcctggaac
    44041 tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag tgacggtcca
    44101 agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt tcaatttaac
    44161 cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc gattatttgc
    44221 tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg gcacagcatt
    44281 tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc gccaatcaat
    44341 gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt tactaaatgc
    44401 tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag atgcttcagg
    44461 aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg ggaataaatt
    44521 agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg agcgacttga
    44581 aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa cggaatacaa
    44641 aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca catccccttt
    44701 ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct aaacttaaca
    44761 aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct ggtatctgtc
    44821 ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg gaagaagcgt
    44881 tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca tctgttgtcc
    44941 atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt gcttcttcag
    45001 ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct tcgatgatcg
    45061 cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa tatttggttg
    45121 cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca ggattgccag
    45181 tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa gaaggatatt
    45241 tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca agtgtattga
    45301 aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga tttggttcgc
    45361 ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc atgactgatg
    45421 cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt ttcaagaact
    45481 gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct aaaatagatg
    45541 agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact cggacagcaa
    45601 aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt tgaggcgcat
    45661 ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg gaagaaggtc
    45721 cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta cctgcatatt
    45781 gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat acgtaataag
    45841 tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat ggttttccaa
    45901 gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt gtaggcaata
    45961 cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga ttctctatac
    46021 cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca ttcgatgaac
    46081 cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat ccagatgggt
    46141 ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt gaattatttg
    46201 aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc ggatctggtg
    46261 tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc ggtccagctt
    46321 taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt gcatcataat
    46381 ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca gctggcgtaa
    46441 tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt tccgtaatat
    46501 ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc ttaccaatca
    46561 aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct gatggatcag
    46621 ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt ccagttgggt
    46681 cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc gtatctgttg
    46741 tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc gacaaaacag
    46801 acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt ttcataataa
    46861 actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag acattttttg
    46921 gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg tattctaaat
    46981 aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt tcattttatt
    47041 gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta tgcatatgta
    47101 aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat cctaaaaaga
    47161 aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta actattcata
    47221 agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta cgactagact
    47281 aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag gcaatgtggt
    47341 cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta cagctccacc
    47401 agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg caattccgta
    47461 aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac cggagatcga
    47521 acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag ccattccaaa
    47581 attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga tattactgat
    47641 catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc aaccaccagc
    47701 gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg cggcagctaa
    47761 accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat
    47821 aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat attttttgag
    47881 gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga cagtagacgt
    47941 atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc cactgtctga
    48001 aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg caatatatcc
    48061 attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa aataaaagac
    48121 ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa gccagccaat
    48181 cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca gcggaatgat
    48241 cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta gcttttcttt
    48301 cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc
    48361 catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag ggatttgctg
    48421 ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa gccgagtcgc
    48481 acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca gcatatgaat
    48541 ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa gaccactcca
    48601 ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag
    48661 ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt atctggtaac
    48721 aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc cgccaattca
    48781 atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa acttggaaga
    48841 ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat gcctgcatcc
    48901 ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg
    48961 attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta gactgctgat
    49021 tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg cacagcaaca
    49081 gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc tggctgaagt
    49141 gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac ggcatccact
    49201 ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc ggcactgcct
    49261 agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc tgcgaccaaa
    49321 ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc atttggtata
    49381 atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg tgcaggattc
    49441 aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt cgcatctaag
    49501 ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt gttttttagg
    49561 acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact attttctcct
    49621 gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc agctatccca
    49681 gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc gctgtcagcc
    49741 ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg
    49801 gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg
    49861 ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac tgtaaccgtg
    49921 ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat tttttaaaac
    49981 gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca
    50041 agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt
    50101 tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc aattcgaaga
    50161 ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat
    50221 agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat ccgcgatata
    50281 atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt
    50341 ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt
    50401 cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat
    50461 tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga ctaagcaagt
    50521 gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt
    50581 cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga
    50641 ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga
    50701 gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga
    50761 cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat
    50821 tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag
    50881 caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt
    50941 tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc
    51001 cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat
    51061 ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc
    51121 cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc
    51181 agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca
    51241 agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg cagtagcata
    51301 tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac gccggcgaag
    51361 ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga tagtcaacat
    51421 attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg gaggtacaga
    51481 agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg agatgtctac
    51541 gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca aatcattgtt
    51601 tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga tctctttttg
    51661 catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt cctttaaatc
    51721 attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat cataaatcgt
    51781 ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc agtggtaaaa
    51841 taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat cgtgaaaatg
    51901 gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac aaaatgggag
    51961 aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc gaattagtga
    52021 ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta ggtgggatgg
    52081 atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa ttttcagata
    52141 aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc tacaatctcg
    52201 ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc
    52261 ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat aactttccgt
    52321 cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc
    52381 ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag
    52441 tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg atcattacac
    52501 ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat gctaaagtaa
    52561 ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg tagggggatt
    52621 ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa tctaaaggac
    52681 gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta ggtgtcaaaa
    52741 ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca ggactttctg
    52801 atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga
    52861 tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact ggcaaaaacg
    52921 aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta caattaactg
    52981 atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag
    53041 ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa aaagagtata
    53101 ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga ggatacgcga
    53161 acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt tttaaaacag
    53221 atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat acgtacagca
    53281 aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc
    53341 gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta gaaccggcac
    53401 agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg ttagaagatg
    53461 ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa
    53521 caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa gaaaggcaat
    53581 tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga aatgcagaaa
    53641 aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa aatgtcggtt
    53701 ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt
    53761 tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg gtagaagaaa
    53821 accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa
    53881 aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga acgatattag
    53941 gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat gatattggga
    54001 gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca ttcttcattg
    54061 ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg cctgctgcat
    54121 tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac atcactccgc
    54181 tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc cgatataagt
    54241 caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg gttgcaggcg
    54301 tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg atcattgact
    54361 atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa gcagcggata
    54421 tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa accgtagaat
    54481 tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg
    54541 atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc ttgcctaaaa
    54601 caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca ggagatcata
    54661 tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa
    54721 actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt actgatcaat
    54781 cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa gaagaagaaa
    54841 tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc atgtccaaac
    54901 aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta atctttgtcg
    54961 tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc aatatttccg
    55021 agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa gaagtcacga
    55081 tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga ggatttggtg
    55141 taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat gtcatctttc
    55201 cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc gtatttacgg
    55261 ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt gatgcgttga
    55321 aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt attagagaaa
    55381 caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg aaggacagct
    55441 gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc aaaaagaata
    55501 ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc gtagataaaa
    55561 gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa caagaggata
    55621 gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta gaatgtataa
    55681 agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac ttgtatatat
    55741 aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca tttaaagaca
    55801 aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat ggaattggag
    55861 atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc gacatgattt
    55921 ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc tctgattaca
    55981 cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata gccgaagcaa
    56041 agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg acagaacatg
    56101 aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc attttaagag
    56161 aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca tttggagata
    56221 cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac tggcggaacc
    56281 aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa ggggtaaaag
    56341 gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa aataataaag
    56401 aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat ctaaaagaat
    56461 tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa atgagctcta
    56521 cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta tcaatggtct
    56581 ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct actcctccat
    56641 ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg agtgagggta
    56701 acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc aatcgatttg
    56761 tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg atccatttga
    56821 accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac ccagactatt
    56881 catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta ctccttgaac
    56941 aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc gataactctc
    57001 gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg aaaccttggt
    57061 taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct tctgaagatt
    57121 ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca atcgttgcac
    57181 aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt cttcgccaat
    57241 ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca aggatcgcca
    57301 tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat actgttcttc
    57361 atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat taaaacaaaa
    57421 acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg ttttatttaa
    57481 aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca taaaatttat
    57541 tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact aacgtataga
    57601 taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca agtaaaacga
    57661 ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga atcaccaagt
    57721 caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc ataaaagtag
    57781 agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca atatatggaa
    57841 tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat ccgatcagta
    57901 agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc ccctttagaa
    57961 atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta cgaaatgctg
    58021 gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa aggaaaaaag
    58081 cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa aaaatgttgc
    58141 tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata tcagacgtca
    58201 cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt ttctccaaaa
    58261 attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg atcaatgtct
    58321 ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat aaaaataaaa
    58381 taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc aaacgtttga
    58441 tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc aagatagtag
    58501 cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa taaatgatca
    58561 gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct aaaaagcgga
    58621 tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta tctataactt
    58681 cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca aaaatatcgt
    58741 caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac agttctttta
    58801 accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg cgaaaagcga
    58861 atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa acgattgata
    58921 ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc aagtgggaaa
    58981 gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat gtatgatttt
    59041 acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc agggactgat
    59101 tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac agatatcttt
    59161 gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac attacaagaa
    59221 ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg aagtgaagaa
    59281 ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct agacccacat
    59341 gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga tcagctgatt
    59401 gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta taccgagaaa
    59461 cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca aagatctttt
    59521 gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa tcaagtatcg
    59581 attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga actgaaacac
    59641 tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa agatagtatt
    59701 gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc tttggaagga
    59761 ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga agcaaacctc
    59821 gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt gacccccaaa
    59881 aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga tcgctctctt
    59941 tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg gacttttgat
    60001 cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga atataaagaa
    60061 tattatacga agggctatca aacagatatt gcttcaatca agatcagtga gaaagagatg
    60121 gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt agggaaagaa
    60181 atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc agaagataag
    60241 gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc ggctgactcc
    60301 gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa agaaatggag
    60361 aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca ggaaatgctt
    60421 ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt tagtcggtcc
    60481 aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga ggctgggatg
    60541 gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg cacattttgt
    60601 gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt ctcgatttct
    60661 ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag tttatgtggc
    60721 gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt gatctcatca
    60781 ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc ttcgaaaatt
    60841 ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt gacgatataa
    60901 tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg ggaagtcaca
    60961 tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc tacaccatcc
    61021 atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga agttctgatc
    61081 ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata actgtctaag
    61141 ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg ttttggatcg
    61201 tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg tatataaatc
    61261 gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg attggtcttt
    61321 gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt ttttgttgag
    61381 aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt gatcacacga
    61441 atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt ctttgcagca
    61501 ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag gtttaccgta
    61561 tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa ccctttttcc
    61621 tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt catattatcc
    61681 aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa tatcgtttga
    61741 tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg aagagaaaaa
    61801 agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt ttctagatct
    61861 ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc taatttctta
    61921 ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata tagaatgaaa
    61981 aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt ataaaaaata
    62041 atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg gcggtccaca
    62101 gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc agataaaaaa
    62161 gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac cgaagatttc
    62221 aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt caataaccca
    62281 aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa agtaagcatc
    62341 agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac ccatccaatc
    62401 aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat gtgatggtca
    62461 atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca taaaaacata
    62521 aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt atttttttaa
    62581 tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata caaaaaagtc
    62641 agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct ccaaaattca
    62701 tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca agtagcaaat
    62761 aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt agaaacatgc
    62821 tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg attgctgtac
    62881 ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag accatcattg
    62941 ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat aaataaagta
    63001 gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca tatctgcgga
    63061 tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc accagagaaa
    63121 agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag acaaatttta
    63181 cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct tctaataatt
    63241 tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta tgctaaaaaa
    63301 aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg tcatgatctc
    63361 ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt tgatacgaaa
    63421 agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa attactgacc
    63481 ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa gtcggcgaaa
    63541 catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata gaaaaaatct
    63601 atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat gataccgctc
    63661 ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa taaaaacaga
    63721 taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag ttacaaggaa
    63781 taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg aggtgttggc
    63841 aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac agcctttatt
    63901 ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag gagcagataa
    63961 agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt ttattacagg
    64021 aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga aaagcacact
    64081 aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg tggaagataa
    64141 tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat tacagatttt
    64201 ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg acaacgtact
    64261 agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt tagggcgtgt
    64321 ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga aggagttaga
    64381 agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat cctttgaaac
    64441 tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac aagatcctga
    64501 actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg aacagtttga
    64561 tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg atccggcatg
    64621 tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag aagaggcaga
    64681 tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct ttacaactaa
    64741 ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag cagcatcttg
    64801 cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat aaaattgcca
    64861 taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga tcaaaatcga
    64921 atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga taaaggcaag
    64981 aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag aaatacaata
    65041 atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat tagtgtaggc
    65101 acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag caatcaaaat
    65161 cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa aaatcactgc
    65221 ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa aataatcaat
    65281 tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata cttttttacc
    65341 aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat tgccagtttg
    65401 agcagaggca aaagcaccat attggatata agtataactg tccatggctc ctccgataaa
    65461 tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt ttttcatcac
    65521 cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa aaggaaaaat
    65581 tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt ctgttagtat
    65641 aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa attcaaagaa
    65701 gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat tacaaaaaga
    65761 attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc tacaagaaga
    65821 aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg aggaagaagt
    65881 acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga aacgttccta
    65941 taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga aacatcttcg
    66001 aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag ctgtttatct
    66061 gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta caaggaacgc
    66121 tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg ctgtccatat
    66181 cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa aatttcctga
    66241 agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa aagtgaatgc
    66301 tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat atggagaaaa
    66361 aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag aagaaatggt
    66421 tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc cgatatcaga
    66481 acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc aattgctaga
    66541 gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga tccagcatta
    66601 tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta atccacttca
    66661 atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg ttgttggaga
    66721 tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca agaactttga
    66781 aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc gggcagctat
    66841 tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac caaaggcgat
    66901 gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg atactaaagc
    66961 agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg ggaaatataa
    67021 ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc aatctctgct
    67081 gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta tctttggtat
    67141 caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac tgaatctgca
    67201 aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact gctacctgaa
    67261 aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg ttgcagatta
    67321 tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag agtttgagga
    67381 agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa gctatcttca
    67441 tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac gagaagaagt
    67501 aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc aagcgacagc
    67561 taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg cactatgtat
    67621 ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga ttggttgtaa
    67681 cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact ttaatgaaat
    67741 aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg ttgcgatgac
    67801 tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa gaaaattgaa
    67861 agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt gatagaagag
    67921 agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa aattgcttct
    67981 caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac acagtttatt
    68041 cggaaaaggg aggtgtgaca agaattttgt cacacctc
//
LOCUS       DAFCLG010000006        51400 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFCLG010000006 DAFCLG010000000
VERSION     DAFCLG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.006"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            125..1168
                     /locus_tag="LND56_000384"
     CDS             125..1168
                     /locus_tag="LND56_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK6742337.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="LND56_000385"
     CDS             complement(1230..1694)
                     /locus_tag="LND56_000385"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK6742338.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="LND56_000386"
     CDS             1940..2887
                     /locus_tag="LND56_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6742339.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3098..5170)
                     /locus_tag="LND56_000387"
     CDS             complement(3098..5170)
                     /locus_tag="LND56_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6742340.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5103..5480)
                     /locus_tag="LND56_000388"
     CDS             complement(5103..5480)
                     /locus_tag="LND56_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBK6742341.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5609..6187)
                     /locus_tag="LND56_000389"
     CDS             complement(5609..6187)
                     /locus_tag="LND56_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6742342.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            6319..7611
                     /locus_tag="LND56_000390"
     CDS             6319..7611
                     /locus_tag="LND56_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6742343.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(7671..8789)
                     /locus_tag="LND56_000391"
     CDS             complement(7671..8789)
                     /locus_tag="LND56_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBK6742344.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="LND56_000392"
     CDS             complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="LND56_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBK6742345.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="LND56_000393"
     CDS             complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="LND56_000393"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK6742346.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            11995..12594
                     /locus_tag="LND56_000394"
     CDS             11995..12594
                     /locus_tag="LND56_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBK6742347.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="LND56_000395"
     CDS             complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="LND56_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBK6742348.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(14289..14633)
                     /locus_tag="LND56_000396"
     CDS             complement(14289..14633)
                     /locus_tag="LND56_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBK6742349.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14770..15915)
                     /locus_tag="LND56_000397"
     CDS             complement(14770..15915)
                     /locus_tag="LND56_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6742350.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(16095..17069)
                     /locus_tag="LND56_000398"
     CDS             complement(16095..17069)
                     /locus_tag="LND56_000398"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6742351.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17149..18024)
                     /locus_tag="LND56_000399"
     CDS             complement(17149..18024)
                     /locus_tag="LND56_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBK6742352.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18154..20061
                     /locus_tag="LND56_000400"
     CDS             18154..20061
                     /locus_tag="LND56_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBK6742353.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20602..23880)
                     /locus_tag="LND56_000401"
     CDS             complement(20602..23880)
                     /locus_tag="LND56_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBK6742354.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(24151..24696)
                     /locus_tag="LND56_000402"
     CDS             complement(24151..24696)
                     /locus_tag="LND56_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6742355.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24708..25400)
                     /locus_tag="LND56_000403"
     CDS             complement(24708..25400)
                     /locus_tag="LND56_000403"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK6742356.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25419..25703)
                     /locus_tag="LND56_000404"
     CDS             complement(25419..25703)
                     /locus_tag="LND56_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742357.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25716..26279)
                     /locus_tag="LND56_000405"
     CDS             complement(25716..26279)
                     /locus_tag="LND56_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6742358.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26507..27169
                     /locus_tag="LND56_000406"
     CDS             26507..27169
                     /locus_tag="LND56_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBK6742359.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            27293..28489
                     /locus_tag="LND56_000407"
     CDS             27293..28489
                     /locus_tag="LND56_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBK6742360.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28590..29966)
                     /locus_tag="LND56_000408"
     CDS             complement(28590..29966)
                     /locus_tag="LND56_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6742361.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(30003..30869)
                     /locus_tag="LND56_000409"
     CDS             complement(30003..30869)
                     /locus_tag="LND56_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBK6742362.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30958..31983)
                     /locus_tag="LND56_000410"
     CDS             complement(30958..31983)
                     /locus_tag="LND56_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6742363.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(32001..32675)
                     /locus_tag="LND56_000411"
     CDS             complement(32001..32675)
                     /locus_tag="LND56_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6742364.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32859..33752)
                     /locus_tag="LND56_000412"
     CDS             complement(32859..33752)
                     /locus_tag="LND56_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6742365.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(33826..35523)
                     /locus_tag="LND56_000413"
     CDS             complement(33826..35523)
                     /locus_tag="LND56_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6742366.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(35516..36829)
                     /locus_tag="LND56_000414"
     CDS             complement(35516..36829)
                     /locus_tag="LND56_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK6742367.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(36829..40347)
                     /locus_tag="LND56_000415"
     CDS             complement(36829..40347)
                     /locus_tag="LND56_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK6742368.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(40640..41098)
                     /locus_tag="LND56_000416"
     CDS             complement(40640..41098)
                     /locus_tag="LND56_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6742369.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(41396..41914)
                     /locus_tag="LND56_000417"
     CDS             complement(41396..41914)
                     /locus_tag="LND56_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK6742370.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(41916..42533)
                     /locus_tag="LND56_000418"
     CDS             complement(41916..42533)
                     /locus_tag="LND56_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK6742371.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(42530..43384)
                     /locus_tag="LND56_000419"
     CDS             complement(42530..43384)
                     /locus_tag="LND56_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6742372.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(43381..44292)
                     /locus_tag="LND56_000420"
     CDS             complement(43381..44292)
                     /locus_tag="LND56_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6742373.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(44333..45667)
                     /locus_tag="LND56_000421"
     CDS             complement(44333..45667)
                     /locus_tag="LND56_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6742374.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            45875..46705
                     /locus_tag="LND56_000422"
     CDS             45875..46705
                     /locus_tag="LND56_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBK6742375.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(46949..47231)
                     /locus_tag="LND56_000423"
                     /pseudo
     CDS             complement(46949..47231)
                     /locus_tag="LND56_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(47389..47583)
                     /locus_tag="LND56_000424"
     CDS             complement(47389..47583)
                     /locus_tag="LND56_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742376.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(48088..48273)
                     /locus_tag="LND56_000425"
     CDS             complement(48088..48273)
                     /locus_tag="LND56_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742377.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(49123..49464)
                     /locus_tag="LND56_000426"
     CDS             complement(49123..49464)
                     /locus_tag="LND56_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6742378.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(49464..49694)
                     /locus_tag="LND56_000427"
     CDS             complement(49464..49694)
                     /locus_tag="LND56_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6742379.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            49981..50319
                     /locus_tag="LND56_000428"
     CDS             49981..50319
                     /locus_tag="LND56_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742380.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(50524..50763)
                     /locus_tag="LND56_000429"
     CDS             complement(50524..50763)
                     /locus_tag="LND56_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK6742381.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(50739..51086)
                     /locus_tag="LND56_000430"
     CDS             complement(50739..51086)
                     /locus_tag="LND56_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK6742382.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14933 a        10883 c         9059 g        16525 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     3121 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     3181 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     3241 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     3301 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     3361 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     3421 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     3481 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     3541 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     3601 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     3661 gcaatccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     3721 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     3781 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     3841 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     3901 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     3961 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     4021 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     4081 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     4141 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     4201 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     4261 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     4321 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     4381 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     4441 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     4501 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     4561 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     4621 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     4681 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     4741 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     4801 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     4861 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     4921 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     4981 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     5041 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     5101 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     5161 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     5221 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     5281 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     5341 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     5401 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     5461 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     5521 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     5581 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     5641 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     5701 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     5761 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     5821 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     5881 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     5941 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     6001 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     6061 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     6121 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     6181 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     6241 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     6301 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     6361 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     6421 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     6481 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     6541 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     6601 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     6661 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     6721 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     6781 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     6841 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     6901 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     6961 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     7021 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     7081 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     7141 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     7201 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     7261 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     7321 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     7381 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     7441 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     7501 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     7561 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     7621 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     7681 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     7741 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     7801 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     7861 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     7921 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     7981 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     8041 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     8101 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     8161 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     8221 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     8281 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     8341 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     8401 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     8461 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     8521 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     8581 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     8641 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     8701 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     8761 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     8821 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     8881 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     8941 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     9001 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     9061 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     9121 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     9181 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     9241 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     9301 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     9361 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     9421 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     9481 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     9541 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     9601 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     9661 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     9721 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     9781 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     9841 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     9901 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     9961 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    10021 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    10081 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    10141 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    10201 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    10261 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    10321 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    10381 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    10441 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    10501 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    10561 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    10621 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    10681 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    10741 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    10801 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    10861 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    10921 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    10981 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    11041 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    11101 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
    11161 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    11221 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    11281 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    11341 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    11401 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    11461 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    11521 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    11581 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    11641 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    11701 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    11761 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    11821 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    11881 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    11941 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    12001 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    12061 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    12121 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    12181 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    12241 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    12301 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    12361 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    12421 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    12481 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    12541 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    12601 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    12661 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    12721 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    12781 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    12841 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    12901 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    12961 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    13021 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    13081 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    13141 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    13201 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    13261 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    13321 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    13381 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    13441 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    13501 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    13561 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    13621 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    13681 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    13741 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    13801 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    13861 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    13921 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    13981 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    14041 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    14101 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    14161 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    14221 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    14281 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    14341 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    14401 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    14461 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    14521 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    14581 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    14641 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    14701 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    14761 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    14821 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    14881 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    14941 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    15001 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    15061 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    15121 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    15181 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    15241 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    15301 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    15361 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    15421 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    15481 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    15541 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    15601 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    15661 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    15721 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    15781 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    15841 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    15901 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    15961 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    16021 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    16081 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    16141 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    16201 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    16261 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    16321 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    16381 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    16441 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    16501 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    16561 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    16621 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    16681 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    16741 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    16801 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    16861 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    16921 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    16981 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    17041 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    17101 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    17161 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    17221 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    17281 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    17341 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    17401 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    17461 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    17521 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    17581 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    17641 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    17701 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    17761 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    17821 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    17881 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    17941 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    18001 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    18061 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    18121 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    18181 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    18241 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    18301 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    18361 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    18421 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    18481 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    18541 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    18601 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    18661 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    18721 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    18781 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    18841 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    18901 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    18961 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    19021 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    19081 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    19141 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    19201 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    19261 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    19321 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    19381 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    19441 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    19501 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    19561 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    19621 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    19681 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    19741 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    19801 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    19861 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    19921 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    19981 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    20041 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    20101 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    20161 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    20221 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    20281 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    20341 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    20401 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    20461 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    20521 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    20581 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    20641 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    20701 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    20761 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    20821 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    20881 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    20941 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    21001 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    21061 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    21121 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    21181 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    21241 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    21301 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    21361 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    21421 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    21481 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    21541 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    21601 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    21661 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    21721 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    21781 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    21841 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    21901 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    21961 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    22021 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    22081 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    22141 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    22201 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    22261 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    22321 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    22381 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    22441 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    22501 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    22561 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    22621 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    22681 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    22741 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    22801 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    22861 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    22921 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    22981 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    23041 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    23101 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    23161 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    23221 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    23281 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    23341 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    23401 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    23461 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    23521 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    23581 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    23641 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    23701 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    23761 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    23821 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    23881 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    23941 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    24001 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    24061 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    24121 ctttttattt tatcaaacca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    24181 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
    24241 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    24301 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    24361 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    24421 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    24481 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    24541 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    24601 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    24661 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    24721 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    24781 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    24841 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    24901 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    24961 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    25021 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    25081 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    25141 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    25201 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    25261 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    25321 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    25381 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    25441 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    25501 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    25561 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    25621 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    25681 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    25741 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    25801 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    25861 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    25921 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    25981 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    26041 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    26101 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    26161 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    26221 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    26281 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    26341 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    26401 tctttagtcc gatactcttt atagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    26461 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    26521 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    26581 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    26641 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    26701 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    26761 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    26821 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    26881 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    26941 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    27001 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    27061 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    27121 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    27181 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    27241 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    27301 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    27361 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    27421 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    27481 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    27541 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    27601 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    27661 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    27721 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    27781 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    27841 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    27901 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    27961 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    28021 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    28081 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    28141 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    28201 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    28261 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    28321 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    28381 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    28441 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    28501 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    28561 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    28621 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    28681 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
    28741 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    28801 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    28861 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    28921 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    28981 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    29041 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    29101 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    29161 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    29221 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    29281 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    29341 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    29401 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    29461 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    29521 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    29581 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    29641 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    29701 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    29761 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    29821 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    29881 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    29941 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    30001 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
    30061 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
    30121 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
    30181 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
    30241 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
    30301 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
    30361 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
    30421 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
    30481 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
    30541 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
    30601 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
    30661 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
    30721 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
    30781 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
    30841 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
    30901 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
    30961 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
    31021 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
    31081 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
    31141 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
    31201 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
    31261 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
    31321 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
    31381 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
    31441 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
    31501 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
    31561 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
    31621 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
    31681 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
    31741 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
    31801 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
    31861 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
    31921 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
    31981 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
    32041 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
    32101 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
    32161 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
    32221 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
    32281 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
    32341 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
    32401 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
    32461 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
    32521 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
    32581 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
    32641 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
    32701 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
    32761 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
    32821 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
    32881 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
    32941 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
    33001 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
    33061 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
    33121 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
    33181 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
    33241 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
    33301 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
    33361 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
    33421 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
    33481 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
    33541 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
    33601 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
    33661 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
    33721 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
    33781 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
    33841 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
    33901 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
    33961 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
    34021 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
    34081 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    34141 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    34201 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    34261 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    34321 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    34381 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    34441 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    34501 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    34561 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    34621 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    34681 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    34741 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    34801 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    34861 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    34921 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    34981 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    35041 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    35101 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    35161 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    35221 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    35281 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    35341 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    35401 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    35461 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    35521 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    35581 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    35641 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    35701 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    35761 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    35821 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    35881 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    35941 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    36001 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    36061 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    36121 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    36181 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    36241 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    36301 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    36361 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    36421 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    36481 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    36541 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    36601 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    36661 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    36721 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    36781 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    36841 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    36901 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    36961 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    37021 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    37081 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    37141 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    37201 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    37261 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    37321 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    37381 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    37441 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    37501 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    37561 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    37621 taaatcgaca tacttttcta cacctacggc acgagaatag aagtatccga tcccaggcaa
    37681 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    37741 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    37801 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    37861 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    37921 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    37981 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    38041 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    38101 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    38161 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    38221 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    38281 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    38341 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    38401 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    38461 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    38521 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    38581 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    38641 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    38701 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    38761 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    38821 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    38881 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    38941 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    39001 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    39061 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    39121 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    39181 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    39241 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    39301 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    39361 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    39421 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    39481 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    39541 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    39601 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    39661 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    39721 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    39781 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    39841 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    39901 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    39961 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    40021 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    40081 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    40141 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    40201 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    40261 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    40321 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    40381 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    40441 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    40501 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    40561 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa
    40621 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    40681 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    40741 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    40801 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    40861 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    40921 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    40981 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    41041 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    41101 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    41161 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    41221 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    41281 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    41341 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    41401 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    41461 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    41521 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    41581 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    41641 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    41701 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    41761 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    41821 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    41881 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    41941 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    42001 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    42061 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    42121 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    42181 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    42241 atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    42301 taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca
    42361 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    42421 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    42481 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    42541 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    42601 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    42661 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    42721 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    42781 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    42841 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    42901 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    42961 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    43021 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    43081 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    43141 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    43201 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    43261 atgaagtaaa caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga
    43321 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    43381 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    43441 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    43501 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    43561 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    43621 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    43681 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    43741 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    43801 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    43861 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    43921 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    43981 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    44041 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    44101 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    44161 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    44221 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    44281 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    44341 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    44401 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    44461 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    44521 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    44581 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    44641 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    44701 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    44761 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    44821 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    44881 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    44941 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    45001 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    45061 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    45121 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    45181 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    45241 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    45301 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    45361 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    45421 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    45481 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    45541 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    45601 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    45661 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    45721 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    45781 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    45841 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    45901 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    45961 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    46021 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    46081 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    46141 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    46201 gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca atccatggag
    46261 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    46321 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    46381 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    46441 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    46501 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    46561 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    46621 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    46681 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    46741 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    46801 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    46861 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    46921 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    46981 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    47041 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    47101 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    47161 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    47221 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    47281 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
    47341 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
    47401 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
    47461 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
    47521 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
    47581 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
    47641 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
    47701 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
    47761 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
    47821 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
    47881 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
    47941 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
    48001 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
    48061 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
    48121 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
    48181 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
    48241 gatggttagg aatcctttag aatttttact cattcataga cacttcctta aataactttt
    48301 attatagaag gtattgtaaa taataatatt gacaaaaagt gactcaaaat aaaaaaaaac
    48361 ttacattata ctggaatgaa ctgtattgat agtgtaccta aaagctctac tgacataaga
    48421 tcgtgtaatc ggtaaagtaa ttttattaat tatacgggat gtaatcaatt agagaataaa
    48481 ctaatcataa aaggtacttt tacattcata attgtcgctt tgagattcta ctttggttca
    48541 ctatctgacc caattgcaca ttttcgatca ttacaagtat tggtgtcgtt agaacaacta
    48601 aataaaaact agcggtagat ttttcagaat ctatgctaat ttctgtatgg atgttccagt
    48661 gatggcaaat ttctcttttc attgtttttc ttgtcgcatg atatgccttt ttgtgttatt
    48721 cagttaaact atagaaattc tttcctagac agatcttgat cccaaatttt acgtttttga
    48781 gcatcgatat agtttaaaat gacattactc atatctaaag agtttagatt ttttaggact
    48841 aaaactgtca gttgttcttt ttcttctttc tgttaaagta ttgtcgcgac ttttcgtata
    48901 cgttaagata tgtcgtgaaa aaacaagttt tgtgacacaa aaaaacgaat gacttgagga
    48961 aatcacctca atcattcgtt taaaatgcta tatattcttt agtttaagat acggaaatta
    49021 atgccatcga ccacactagt agaatgaacg acatttactt ggcctttttt taatttcgtt
    49081 atgtttagca tataaaaata ttatagagtt tacttacaat gcttaaaaca tatactgtat
    49141 aatctgatca attttagcca tgtcttgcaa tggtaagttt tctatttttt tgagttttcg
    49201 ttccttaaaa tcaagagatt ttaattgtgt aataattact tgtcctttta tatctagctc
    49261 ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg gacatatgac
    49321 cgaaaacatt gttttacggt taaattcata acgagagacg actagtcctg gtcgtctttt
    49381 ttgaatttct tttcctacgg agggatcaaa atcaatccat acaatatcac cttttttagg
    49441 aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt taaatcttcc
    49501 cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc ttcaattttt
    49561 ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg gttttctgaa
    49621 ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg taatctagat
    49681 tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac actaagtaac
    49741 gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat atcttattgg
    49801 atataaatag aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa
    49861 gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc
    49921 ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc
    49981 atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga
    50041 aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt
    50101 caaatagaaa cattggttca acgaatttca actagacaag actttgctat tgtagaacgt
    50161 gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg
    50221 gataaatggc aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta
    50281 actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata
    50341 aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg
    50401 actgtgaccc tatagatgca gtatatgcag cttatgagta tttcttctgt tctctttgtt
    50461 tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt
    50521 atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa
    50581 agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc
    50641 atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt
    50701 ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa
    50761 cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat
    50821 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
    50881 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
    50941 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
    51001 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
    51061 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
    51121 ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg
    51181 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
    51241 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
    51301 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
    51361 ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa
//
LOCUS       DAFCLG010000007        51261 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFCLG010000007 DAFCLG010000000
VERSION     DAFCLG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.007"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1135
                     /locus_tag="LND56_000431"
     CDS             290..1135
                     /locus_tag="LND56_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6742383.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="LND56_000432"
     CDS             1416..2636
                     /locus_tag="LND56_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBK6742384.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="LND56_000433"
     CDS             2633..5353
                     /locus_tag="LND56_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6742385.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="LND56_000434"
     CDS             5337..6281
                     /locus_tag="LND56_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6742386.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="LND56_000435"
     CDS             complement(6350..7360)
                     /locus_tag="LND56_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6742387.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="LND56_000436"
     CDS             complement(7540..7884)
                     /locus_tag="LND56_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6742388.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="LND56_000437"
     CDS             complement(7877..8311)
                     /locus_tag="LND56_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBK6742389.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="LND56_000438"
     CDS             8449..9186
                     /locus_tag="LND56_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742390.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="LND56_000439"
     CDS             9179..10390
                     /locus_tag="LND56_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742391.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="LND56_000440"
     CDS             10594..11376
                     /locus_tag="LND56_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBK6742392.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="LND56_000441"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="LND56_000441"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK6742393.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LND56_000442"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LND56_000442"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK6742394.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="LND56_000443"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="LND56_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK6742395.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="LND56_000444"
     CDS             14978..16027
                     /locus_tag="LND56_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBK6742396.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="LND56_000445"
     CDS             16024..16302
                     /locus_tag="LND56_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK6742397.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="LND56_000446"
     CDS             16277..16747
                     /locus_tag="LND56_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBK6742398.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="LND56_000447"
     CDS             16764..17066
                     /locus_tag="LND56_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742399.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="LND56_000448"
     CDS             17083..17454
                     /locus_tag="LND56_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBK6742400.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="LND56_000449"
     CDS             17426..17785
                     /locus_tag="LND56_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742401.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="LND56_000450"
     CDS             17957..18964
                     /locus_tag="LND56_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6742402.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="LND56_000451"
     CDS             18989..20173
                     /locus_tag="LND56_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK6742403.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="LND56_000452"
     CDS             complement(20610..22193)
                     /locus_tag="LND56_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6742404.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="LND56_000453"
     CDS             complement(22771..22914)
                     /locus_tag="LND56_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK6742405.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="LND56_000454"
     CDS             complement(23137..23616)
                     /locus_tag="LND56_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6742406.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="LND56_000455"
     CDS             complement(23689..23973)
                     /locus_tag="LND56_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742407.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="LND56_000456"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LND56_000456"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="LND56_000457"
     CDS             complement(24317..25603)
                     /locus_tag="LND56_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBK6742408.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="LND56_000458"
     CDS             25741..26412
                     /locus_tag="LND56_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBK6742409.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="LND56_000459"
     CDS             26378..26866
                     /locus_tag="LND56_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBK6742410.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="LND56_000460"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="LND56_000460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK6742411.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="LND56_000461"
     CDS             27827..28591
                     /locus_tag="LND56_000461"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK6742412.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="LND56_000462"
     CDS             28824..31037
                     /locus_tag="LND56_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK6742413.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="LND56_000463"
     CDS             31048..31494
                     /locus_tag="LND56_000463"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK6742414.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="LND56_000464"
     CDS             31665..32924
                     /locus_tag="LND56_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBK6742415.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="LND56_000465"
     CDS             complement(33013..33960)
                     /locus_tag="LND56_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6742416.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="LND56_000466"
     CDS             34354..35646
                     /locus_tag="LND56_000466"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK6742417.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="LND56_000467"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="LND56_000467"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK6742418.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="LND56_000468"
     CDS             37539..38414
                     /locus_tag="LND56_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6742419.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="LND56_000469"
     CDS             38680..40563
                     /locus_tag="LND56_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK6742420.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="LND56_000470"
     CDS             complement(40638..40922)
                     /locus_tag="LND56_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6742421.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="LND56_000471"
     CDS             complement(40912..41646)
                     /locus_tag="LND56_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBK6742422.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="LND56_000472"
     CDS             41775..42398
                     /locus_tag="LND56_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK6742423.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="LND56_000473"
     CDS             42590..43711
                     /locus_tag="LND56_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBK6742424.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="LND56_000474"
     CDS             43718..46846
                     /locus_tag="LND56_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBK6742425.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="LND56_000475"
     CDS             46865..50059
                     /locus_tag="LND56_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBK6742426.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="LND56_000476"
     CDS             complement(50205..50912)
                     /locus_tag="LND56_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6742427.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16775 a         8951 c        10638 g        14897 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccgc cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta a
//
LOCUS       DAFCLG010000008        44115 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFCLG010000008 DAFCLG010000000
VERSION     DAFCLG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.008"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(302..892)
                     /locus_tag="LND56_000477"
     CDS             complement(302..892)
                     /locus_tag="LND56_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBK6742428.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1415..1576
                     /locus_tag="LND56_000478"
     CDS             1415..1576
                     /locus_tag="LND56_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK6742429.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1595..2089
                     /locus_tag="LND56_000479"
     CDS             1595..2089
                     /locus_tag="LND56_000479"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK6742430.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2082..2243
                     /locus_tag="LND56_000480"
     CDS             2082..2243
                     /locus_tag="LND56_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742431.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2727..3750
                     /locus_tag="LND56_000481"
                     /pseudo
     CDS             2727..3750
                     /locus_tag="LND56_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4093..4349
                     /locus_tag="LND56_000482"
                     /pseudo
     CDS             4093..4349
                     /locus_tag="LND56_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4736..4963
                     /locus_tag="LND56_000483"
     CDS             4736..4963
                     /locus_tag="LND56_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK6742432.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4979..6145
                     /locus_tag="LND56_000484"
     CDS             4979..6145
                     /locus_tag="LND56_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBK6742433.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6380..7423
                     /gene="hrcA"
                     /locus_tag="LND56_000485"
     CDS             6380..7423
                     /gene="hrcA"
                     /locus_tag="LND56_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBK6742434.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPIILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7517..8080
                     /gene="grpE"
                     /locus_tag="LND56_000486"
     CDS             7517..8080
                     /gene="grpE"
                     /locus_tag="LND56_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK6742435.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8131..9960
                     /gene="dnaK"
                     /locus_tag="LND56_000487"
     CDS             8131..9960
                     /gene="dnaK"
                     /locus_tag="LND56_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK6742436.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10111..11277
                     /gene="dnaJ"
                     /locus_tag="LND56_000488"
     CDS             10111..11277
                     /gene="dnaJ"
                     /locus_tag="LND56_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK6742437.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11645..12382)
                     /locus_tag="LND56_000489"
     CDS             complement(11645..12382)
                     /locus_tag="LND56_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6742438.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12384..13886)
                     /locus_tag="LND56_000490"
     CDS             complement(12384..13886)
                     /locus_tag="LND56_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBK6742439.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14145..16139
                     /gene="uvrB"
                     /locus_tag="LND56_000491"
     CDS             14145..16139
                     /gene="uvrB"
                     /locus_tag="LND56_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBK6742440.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16149..18965
                     /gene="uvrA"
                     /locus_tag="LND56_000492"
     CDS             16149..18965
                     /gene="uvrA"
                     /locus_tag="LND56_000492"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK6742441.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19228..20493
                     /locus_tag="LND56_000493"
     CDS             19228..20493
                     /locus_tag="LND56_000493"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBK6742442.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20497..21228
                     /locus_tag="LND56_000494"
     CDS             20497..21228
                     /locus_tag="LND56_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBK6742443.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21363..22247
                     /gene="rapZ"
                     /locus_tag="LND56_000495"
     CDS             21363..22247
                     /gene="rapZ"
                     /locus_tag="LND56_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK6742444.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22244..23242
                     /locus_tag="LND56_000496"
     CDS             22244..23242
                     /locus_tag="LND56_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBK6742445.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23276..24211
                     /gene="whiA"
                     /locus_tag="LND56_000497"
     CDS             23276..24211
                     /gene="whiA"
                     /locus_tag="LND56_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBK6742446.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24779..25435
                     /locus_tag="LND56_000498"
     CDS             24779..25435
                     /locus_tag="LND56_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742447.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25614..26179
                     /locus_tag="LND56_000499"
                     /pseudo
     CDS             25614..26179
                     /locus_tag="LND56_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26878..27399)
                     /locus_tag="LND56_000500"
     CDS             complement(26878..27399)
                     /locus_tag="LND56_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6742448.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27597..31277
                     /gene="nifJ"
                     /locus_tag="LND56_000501"
     CDS             27597..31277
                     /gene="nifJ"
                     /locus_tag="LND56_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK6742449.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            31466..32353
                     /locus_tag="LND56_000502"
     CDS             31466..32353
                     /locus_tag="LND56_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBK6742450.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            32350..33519
                     /locus_tag="LND56_000503"
     CDS             32350..33519
                     /locus_tag="LND56_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742451.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            33543..34898
                     /locus_tag="LND56_000504"
     CDS             33543..34898
                     /locus_tag="LND56_000504"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK6742452.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(34970..36775)
                     /gene="glmS"
                     /locus_tag="LND56_000505"
     CDS             complement(34970..36775)
                     /gene="glmS"
                     /locus_tag="LND56_000505"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK6742453.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            37185..37475
                     /locus_tag="LND56_000506"
     CDS             37185..37475
                     /locus_tag="LND56_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742454.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            37488..38513
                     /gene="sppA"
                     /locus_tag="LND56_000507"
     CDS             37488..38513
                     /gene="sppA"
                     /locus_tag="LND56_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK6742455.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            38516..39175
                     /locus_tag="LND56_000508"
     CDS             38516..39175
                     /locus_tag="LND56_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6742456.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            39332..40264
                     /locus_tag="LND56_000509"
     CDS             39332..40264
                     /locus_tag="LND56_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742457.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            40418..41707
                     /locus_tag="LND56_000510"
     CDS             40418..41707
                     /locus_tag="LND56_000510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK6742458.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            41954..43204
                     /gene="clpX"
                     /locus_tag="LND56_000511"
     CDS             41954..43204
                     /gene="clpX"
                     /locus_tag="LND56_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBK6742459.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            43287..43874
                     /locus_tag="LND56_000512"
     CDS             43287..43874
                     /locus_tag="LND56_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK6742460.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43948..44103)
                     /locus_tag="LND56_000513"
     CDS             complement(43948..44103)
                     /locus_tag="LND56_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742461.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT        14668 a         7508 c         9450 g        12489 t
ORIGIN      
        1 cagcgaatcc ttgataatga gctcggatgt gctaacgcta gcgaggcagt ctaactttaa
       61 ctgttagctc ctgccgaaga tcgtcacatc cgaggaggct cattgtcaag gacaatcgga
      121 atagcatacg gaaagaagtt ctgcacctta taaaatttgt caaaaaaact gttgccattc
      181 catctatgaa tataaataga gtgtatcttc tttgataaat gtcagaaaaa gtgtgacaaa
      241 taaccatcag taaaattact tgtcatacta ttgataagga gtatttaatt gaagagaatg
      301 attaggagaa aggatctccg tactttttat agtaatcata gaatccaagt ttgtatagac
      361 gaatcttttt ttctcttatt tcttctgttt caaaatggac tgcttgaaga atcaagtctg
      421 tgaattctat aggagctgtt ccattcgcag taatcaaatt acgatctttc acagcttgtt
      481 gttctataaa atcttcttta ttctgataat tttccgattc taaccaagga aattgcgcat
      541 ttccggtgtg tttgtaacct tgcaataatc cgctctgagc taaataatca actgccccac
      601 aaatagcagc tactggtacg tctgttttta acaatttatc gattatttga tataaatgat
      661 gattttttat gtcccatgaa ttgccaccga tcataataaa aaggtcaact ttttcaggta
      721 tttgttcgat taagtaatcg atttttgttt ttaatcctcc aatggaagtg acctctgatt
      781 ggatagaagc agttttgttt tgccatccct ctttttggtt tagtgtgcta gtaatataag
      841 caccttccca atcggcatat tcctctaata gaaacgtaat cgcagttttc attttcaagc
      901 tctcctttac tatctatgac aaggacaaca gttttaacat acagacattc attaaatttg
      961 gatagcgaca gcgaaaacaa caattcttaa gttttagaca gagtatacag acagtcaact
     1021 taattaaata cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc agcattataa
     1081 cattttgcat acatggatga caaaagaacc aattcccact tgcttgagta caatcttaaa
     1141 agaaactgga aggcaaaaag taattataga aacgaaaaat aaatcatatc gggcactcag
     1201 acatgaaaac aaccctagaa atatataact agtccaatga aaaaaaagcg aatgccgtag
     1261 aaaacaagga ttctgcgtac attcatatct cattttgcga gcatatctgc tattactgtg
     1321 actaaatgtt aatatggtta aaatgaccgt tatatttttt atataaatat cgtataatta
     1381 gtatgaatat agaagaagga aagaaggtgc aatcttggaa cctaaagaca agtatttaga
     1441 ttacgctagg gtcaataaag ataaatttat tgaaacaatc atcaagggaa aaatccctga
     1501 tgatgaaaaa gatgcggtat tcatggctgg aagcccagga gcaggtaaaa cagaggttgc
     1561 actaggttta gcttaaaatt atgacaacca tgttgtgatt gatgcggact attttagagc
     1621 acaatttcca gaatacaatg gaaagaactc aagtgttttt cagaaagctt cttcttggtt
     1681 agttgaacaa tcattaaaat atgtactgga acatggctat tctttcatac tagaggatac
     1741 ttttgctatc cttagtgcag agaaaaatat tattcgagcc cttaaaaata actttcgcgt
     1801 tacgattttc tatgtttacc aagatcccaa agttgcctgg gatttcactc gaaaacgtga
     1861 actagctgaa ggacgtcatg tgccaaaaga aatgtttatc aatgcgtttt ttaagtccag
     1921 agagaatatt gaaaaagtca aaaatagaca tcctgaagta gtccttcaca taatgattaa
     1981 ggattatcaa aacgacattt ctgaagtgca ttatgcagca gataatattc aattagtact
     2041 accaatacaa tatacttcaa aagagttgga ggaggaatta catgactgag aaagaacaag
     2101 tacaacaaat cgtgaagaag tataataaaa gtatcgctga tttatcagaa aacgcctcag
     2161 cgaaagaatt taaaacagta atgaattacg tagcagatga agcaaatcgt aagcagcgaa
     2221 aactagttgg gttggataaa taaatttttt aaaccatatc cttgtaaagt tgagtttttt
     2281 attttaatga attaagacta ttaaatagat aattattgta acttaaaaaa cacagctaaa
     2341 aattagttgt gttttttatt aggtttactt ttctggtata aaaggaacta gctacttttt
     2401 caaaatttct aaatcattca atatgtagcg cttactaaat tattcgccat aaaattttat
     2461 aattggatca ctcattgttt tacctaagtc atatttaagt aataaccggt gtttttacat
     2521 aagaagactt cgggtatttt ttagtttgat tagctctgat atacctataa agaatatgct
     2581 aaggtaagaa tagtaaaaat tcacatagat gaggtataat aatgatattt ttatatatta
     2641 cagataactt gacacaaaat gagttttttt cgatttttca agttatctaa tgcaattata
     2701 ttgatatcat taggagctgc attgttatga atccaacatt gattactaag aaagaattac
     2761 taaaaaaatt agatatttct actggtgtat tagctaattt aattagaaat ggcatgccaa
     2821 aagagggaga aatgtttaat cttgataaga ttattacatg gcgagaaaat tggtctaaaa
     2881 acatattagg tgagttagaa gttggtagag tatatactaa caaagaaatt tcagagaagt
     2941 ttaaatgttc caaacagggt ggaatgagaa gatctcatca aactaatact cttgtactgt
     3001 ttagtgatca aacaggaagt aatgtttata aagataagtg gttaaatggt attttacaat
     3061 ataccggtat ggggctaaaa ggggatcaag tattggataa gaatcaaaat aaagttttag
     3121 ccaattcaaa aagtaatttt gtaaagattc atttatttga aacatttaaa ccaaaagaac
     3181 acacatatct gggagaagta tatttagcag gacaaatata tactgttaat gaaaaagata
     3241 gttcgggcaa cagcagaaaa gtatataaat ttccattagc tttaataaat caagagcaat
     3301 tgattgaaga taaagatata taaccaggaa gaaaatcaaa ctagacatat tcgtaattta
     3361 tctaatgcta aattagagga agaagcaaga aaagttagta attataataa aaaaatggct
     3421 gcaaaatatg ttaacgaaga ggagttttgc ttctcaaaaa caaaaatata taaacgtaat
     3481 cccttcgtat ctgaatatgt taaacgactt gcaaaaggta tttgtcaact ttgccaagaa
     3541 aaagggccat ttataaaaga cggtgtacct tatttacatt gtcatcatat agaatatttg
     3601 tcgcaaggtg gaaaagatgt gattgaaaac tgtatcgcgt tatgtcccaa ttgtcatgct
     3661 agaattcatg aacttgagtt acctgacgat aaagaaaaat tgctgaagat cgttgaaaaa
     3721 agagaaaata gcttttctaa aataccgtaa tttattttta taggacaaat aagctaatgt
     3781 gagctatgcc ttctatataa gactaagttc tattataagt aacgaagtaa ttctcgttcc
     3841 ttcctgtagt aatatttttt aaataaccta taggcttctt caaaagtaca tcctccagta
     3901 aattctaagt actggaggat tctttttcta gtgttgcttt actcatgaaa aagtagttgg
     3961 ctacttcatc aaaatttctg aactttttta atgtataata aagagctcga tcatttacta
     4021 caaggatgct agctcctaca ttttcacagt tttttagcaa gtaaattttt ctaaaatcac
     4081 atcaggaaat gcatgagatt ctttaagaga ggcaattata gggctatgat tacggttcgt
     4141 gtaagtgacg ccaaaaaaga atggctttct tatatggctg aattttatgg gattacttta
     4201 tctgaacttc taaaaaacta ttcgatggaa caactagaag atgaatacaa tcgtcaaacg
     4261 gctgaaattg cccataaaca ttggataaga agtgggaaaa caacagtatt catggaagaa
     4321 tccttaatga atttggtggg ctggaatgac ctgccgttta aaattcacgc ccgaaattca
     4381 gaagcagctc tgattggcaa atacgtgtat tggtagagat agaagataat caattcgttg
     4441 ttatcgctat tcaagtaggg caccgaagaa atgtctatga ttaagagact gtttattttt
     4501 acaaaaaaat aataattgga atctagttaa aactcttgaa gtgagagatt tgactagatt
     4561 tttttatgaa taaaataagg acaaaattgt ttaggaagaa tcatgagtgt aaaaaaatca
     4621 tccaagaagt cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc
     4681 gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa
     4741 aatagctttt catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac
     4801 cgtttatcga gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt
     4861 agagctaggt ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg
     4921 cgattttaag aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat
     4981 gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg
     5041 cgagcatatc tgctattact gtgactttaa taaagttttt ttagaagggc agccagtaga
     5101 tgagtatgtg gaaatgctgc tgaatgaaat gagattaatg ctggaaagtc atcctacagc
     5161 gaatttggaa acactttatg taggaggcgg aacaccaact tctttatctg ctaaacaact
     5221 tgatcgacta ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac
     5281 tgtagaagcg aatcctggtg atttgacccg tgaaaaactt caagtgatga aaaactatgg
     5341 cgtcaatcgt ttatccatgg gagtgcagac atttgacaat cgcttattga aaaaaattgg
     5401 acgtaaacat actgcagaag atgtgtatca aacaatgcaa tttttagaag cagaaaattt
     5461 taccaatgta agcatcgatt tgatttatgc attaccggga caaacactag aaggatatcg
     5521 ggatactttg gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat
     5581 cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga
     5641 tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg
     5701 tcaccaatat gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat
     5761 gtactggaac aatgaccact attttgggtt tggagctggt gctagcggtt atcttggaga
     5821 tattcgttac cgcaacaaag gaccaatcca acactattta cgcccattac gagcaaatga
     5881 acgcccggta ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt
     5941 cttaggacta agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa
     6001 aacaattgaa tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt
     6061 taatgaagaa gaccgtgttt atttgacacc aagagggact tttttgggga acaatgtgtt
     6121 tgagcgcttt ttatttgaaa aatagtttga agaatatttg ttttttatcg aaacttgaaa
     6181 agaaaaagtt tagaaagttt gacaatgagg tattgaagta ttgatataac tggatttaca
     6241 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     6301 gacaggagaa gggcttcttg ctatatttat aagtgaatta gcacttagaa tatatgagtg
     6361 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     6421 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattaatgt ctgagggagt
     6481 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     6541 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     6601 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     6661 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     6721 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     6781 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     6841 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     6901 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     6961 taaacttcgt acggagattc cgatcattct tcataaatat ttccagacga cagaagggat
     7021 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gaaaaggttt atgtcagtgg
     7081 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     7141 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     7201 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     7261 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     7321 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     7381 acttgctgat tactaccgct cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     7441 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     7501 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     7561 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     7621 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagataaata
     7681 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     7741 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     7801 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     7861 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     7921 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     7981 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     8041 tcgcgtgtta agaccaacta tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     8101 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     8161 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     8221 gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     8281 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     8341 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     8401 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     8461 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     8521 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     8581 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     8641 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     8701 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     8761 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     8821 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     8881 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     8941 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     9001 gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag aaatcgacga agttatttta
     9061 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     9121 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     9181 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     9241 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     9301 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     9361 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     9421 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     9481 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     9541 acgatcaaat cttcttcagg tctaacagac gaagaaatcg aacgtatggt gaaagatgca
     9601 gaagcaaacg ctgaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     9661 gacgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     9721 gaagaagtga aaaaagcaga aacagctcgt gacgaattaa aagccgctgt tgaagcaaac
     9781 aacatcgagg aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     9841 gttaaactat acgaacaagc tgcacaacaa caagctcaag aaaatccaga agcagctcaa
     9901 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     9961 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
    10021 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
    10081 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
    10141 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
    10201 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
    10261 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatacga ccaatacggg
    10321 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
    10381 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
    10441 ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
    10501 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
    10561 gaagaaatct gtcatacttg tggtggaaac ggtgcaaaac caggcacaca accaactact
    10621 tgtcataaat gtcatggtgc aggtacgatc aatgtagaac gccaaacgcc acttggacga
    10681 gtgatgagcc gtcaaacttg tgatgtttgt catggcacag ggaaagaaat caaagaacct
    10741 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
    10801 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
    10861 aacggcggac catttggtga tttgtatgtg gtttttcgtg tagaagaaag tgatatcttt
    10921 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
    10981 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
    11041 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
    11101 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
    11161 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
    11221 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
    11281 ttctagtaaa aatataaagt agacttgccc aaattttcta tttctagtaa aaacgctaag
    11341 tcattaataa ttaaaaacca gacgaacaat catacgttcg cctggttttt ttgtagttgt
    11401 catgtttgta ccaacaaaaa agccagcttt agaataagat aatcatcata gtttctgaag
    11461 gtggtatcga ctatgtactt gatatcgtca atgaaccgat ggtactttct tatgcgatgg
    11521 aacaggtaga gcgattgctt gtttgtgcag gagaacaagc gatgcggctg gtacagttaa
    11581 gtgaaaaaac aagaaactaa aaatgcccgg tcgtcatgaa gcgaccgggc atttttggtt
    11641 tttattaaat atttaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
    11701 caaagatttg ttgaggcgtt ccgtcttcta aaaagtttcc gtcgtcaatg aacatgacac
    11761 gattggccac ttctttagcg aagcccattt cgtgcgtaac gataaccatc gtcatacctt
    11821 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcgggg tcgagtgcag
    11881 atgtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
    11941 cacgctgctt ttgtccaccg gaaagcattt caggatacat atctttttta tcttctagac
    12001 caactgtctg cagcaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
    12061 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
    12121 gctggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
    12181 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
    12241 gcaaaaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
    12301 tgatggacac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
    12361 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
    12421 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
    12481 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
    12541 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
    12601 atgcgtatcg cttgcggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
    12661 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
    12721 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
    12781 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
    12841 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
    12901 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
    12961 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
    13021 agaaatgttg attcatcagc agtggaagac tcactggaag tttctgaacc atccgctaca
    13081 tattctttaa taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
    13141 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
    13201 gattcttttt tgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
    13261 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
    13321 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
    13381 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
    13441 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
    13501 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
    13561 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
    13621 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
    13681 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
    13741 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
    13801 tcagcttgtg caacatgtcc aaacgcaagt gtcacaagtc ctgtcattaa gaaaacaaat
    13861 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
    13921 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
    13981 gtttctaaca ttttatgtta taaaacgtaa catgcataat aagataaaaa agtggcgaaa
    14041 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
    14101 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
    14161 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
    14221 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
    14281 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
    14341 taattattgc ccataacaaa acattggctg gtcagctgta cggggaattc aaggaatttt
    14401 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
    14461 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
    14521 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    14581 ccgtttcatg tatttttgga ttaggttcgc cttttgagta ccaaaaacaa gtagtttcga
    14641 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    14701 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    14761 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    14821 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    14881 tggcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    14941 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    15001 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    15061 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    15121 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    15181 acgtaaccat gcctcaagtc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    15241 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    15301 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    15361 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    15421 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    15481 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    15541 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    15601 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    15661 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    15721 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    15781 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    15841 gtctagcgat ggatgaaaca tcaagacgcc gtacgattca gcagaagtac aatgaagaac
    15901 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    15961 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta tgaagaaatg acgaaagaag
    16021 aaaaagatac gttattgatg aaattagaaa aagaaatgaa aaatgcggcg aaagcattgg
    16081 attttgaaac agccgctaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    16141 aggacaacat ggctaacgat aaaattacga ttcacggtgc acgtgcccat aacttgaaaa
    16201 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    16261 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    16321 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    16381 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    16441 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    16501 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    16561 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    16621 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    16681 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    16741 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    16801 gtctatttga ttctttagag gcggctctgc gattggcaga cggctatgct ctggttgacg
    16861 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    16921 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    16981 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    17041 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    17101 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    17161 aattaccgga agagcatcag gatatcatct tgaatgggac agatgatctt ttccatttcc
    17221 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    17281 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    17341 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    17401 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    17461 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    17521 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    17581 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    17641 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    17701 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    17761 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatctgattg
    17821 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    17881 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    17941 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    18001 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    18061 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    18121 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    18181 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    18241 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    18301 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    18361 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    18421 ttccttgtga agtatgtcat ggcaaacgat ataactctga aacattagaa gtgcattaca
    18481 aaggaaaaag cattgctgat attttggaaa tgacagtaga agatgctgta gaattcttca
    18541 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    18601 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    18661 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    18721 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    18781 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    18841 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    18901 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    18961 tataaagagg atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg
    19021 ttttattaat atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta
    19081 tagacaaaaa cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggagt
    19141 ttcctttgat tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa
    19201 caagagatta tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc
    19261 cctatattat taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca
    19321 tacggcaaaa tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa
    19381 attgtcaatg tagaagttat tccaggattt gataaagacc ctgtgtttat cgagacgatg
    19441 cttcgtttgg caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt
    19501 ggtgatggat atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc
    19561 tgtccatata ttgattattc tttatttgaa agactgatca ataaagtaag cttttatgaa
    19621 gtttgtgaag aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta
    19681 gtaaatggac atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca
    19741 aacagtgtgg agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag
    19801 actagagaag agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa
    19861 atgattgtac aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat
    19921 gtagatgaag atcatcaagt tcggatgatg tgtcttggtc atccactgtt agaagatcct
    19981 acaccagcat cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat
    20041 caaaccatta aggcattttt ggaaaaaatc gaatatacag gctttgccaa tttcgatatg
    20101 aagtatgatc ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt
    20161 agcagtttct ttgtgacatt gaacggcttg aatctggctc gtttcgtcac agaagatcga
    20221 gtgtttaaca aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga
    20281 ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa
    20341 aaactagctg agcaaatgat caaagaaaat cgttacggta ctactgtgtt ttatgaaaaa
    20401 gaccggtcaa tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga
    20461 ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta
    20521 ggcggaatgg gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa
    20581 gctacaaaag atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat
    20641 cgtacagctt atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat
    20701 attgagaaac aaaatttatt gcagccgaac ttcattgtgt tgacttgtaa caccgcacac
    20761 tacttttttg aagaattaca agcggcaacg gatattccta tccttcatat gccaagagaa
    20821 gcagcaaatg aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa
    20881 ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa atcttggatt cgaaacagtg
    20941 attcctgata ctgttcttca agaaaaaatc aattatctga tctatcatga gatcaaggaa
    21001 tcggattatt tgaatcaaga gctatattac gaaatcttag aagaagcggt agagcgccta
    21061 aactgtgaaa aagttatctt aggttgcaca gaattgtcat tgatgcatga atttgctgaa
    21121 gataaccatt atccagtgat cgatgcccaa tctattttag cagatcggac gattgaacga
    21181 gcgctagctg aaagaagcaa ggcattggat acagcatcgg aaaagtaaaa aaattggatt
    21241 tggggcaaat atcctaaaag atatttggtc caaatctttt tgtaaaagaa aaagaaaact
    21301 ggtaatgata cgcccggaat gatataattt tactatgcac aatgtgagag gagttcgtaa
    21361 ttatgacaga taatttgcaa ttagtcatca taacaggtat gagcggtgca gggaaaacag
    21421 tagctatcca aagttttgaa gatatgggct atttttgtat cgataatatg ccgcctcgat
    21481 tgattccaaa attctgggaa ttaatcaaag aatcaggcaa agtcacaaaa atggctttag
    21541 gagtggattt acgttctcgt tccttctttg aagaaatcca aaacatgctg atcgaaatcg
    21601 aaaatacgaa cttcatcgac acttctatct tatttttaga tgcttcagac gaagaactag
    21661 tggctcgcta caaagaaaca agacgagcac atccaatggc gatggacggt ttgatcactg
    21721 aagggatacg taaagaacgg gcgatactag acgacttgaa agcccaagct tccgtgatca
    21781 ttgatacgac gacattgaca ccacgacaat tacgtgaaaa aatcaatgag tcattcaaat
    21841 caaagggaga ccaaggattt cggatcgaat ttgtatcatt tggttttaaa tacgggttgc
    21901 cgatcgattc ggatattgtg atggatgtgc gttttttgcc caaccctcat tatatcccgg
    21961 aactaagacc attgaccgga aaagatcagc cagtctatga ttatgtgatg tcctttgatg
    22021 aaacgcaatc attctaccat aaatttttgg atcttctgga aactgtattg cctggataca
    22081 ttaaagaagg aaaaagcagc ttgacagttg caatcggctg tacaggcggt caacaccgtt
    22141 ctgtggcatt gactgaacgt ttggcaaatg ctttagggaa aaactataaa gtcaatgtga
    22201 cacatcggga caaggataaa aggaaagaga cggtgaaccg gtcgtgaaaa tgaaaaccta
    22261 ccggataaga aaaccaaaga tcgtagtggt cggcggtggc actggtctgc cagttatcct
    22321 gaaaagtttg cgaaatcaag gagcagatat cacagcagta gtgactgttg cagatgatgg
    22381 gggaagcagc ggtgcaatca gagaatcgat cgctatggct cctcctggtg acttgagaaa
    22441 tgtcttagtg gcgttatcgg atatgccaca attctatgaa gatattttcc aataccgttt
    22501 caaaaaggaa gatcagtttt tagccaatca tacgatcggt aatctgatca ttgccgcagt
    22561 ttccgagatg cggggaagca cttatgaggc gattcaatta ttagcaaaaa tgatgcatgt
    22621 agatggacat gtttatcctt cttcagaaac tcctttgacc ttgcatgctg tctttaaaga
    22681 cggaacgact gcagtaggag aatctaaaat cgcgatcgac cgtaaaacca ttgaccgtgt
    22741 gtatgtctgc aacacaaatg ataaaagtga agccaaagcg gctcgaaaag tcgtttcagc
    22801 aattatggac gccgacatgg tcgttttggg acccggaagt ttattcacca gtatccttcc
    22861 aaatttagtg ataccagaaa tcggcgaagc aatgctcaag acaactgctg aaaccgtcta
    22921 tatctgtaat atcatgaccc aaaaagggga aacggaacat ttcacagatg ccgatcatat
    22981 acgtgtttta cacaagcatt tgaatcagcc ttttatcgat acagtattgg tcaatacgga
    23041 aaaagtgcct gaagattata tggacccaga gatctatgat gagtatctag tacaagtcgc
    23101 acacgacttc aacggattga gaaatgaagg atgtcgagtg atttccaccg actttttgga
    23161 attgaaagat ggtggtgtct tccacgatgg agacaaagta gtagaagaat tgttccgttt
    23221 agttttcggt tcaaaatatt aagcaattta ttgttggaga aaggaggaga ataggatgtc
    23281 atttgctgca gatgtgaaga aagaactaac tggtttggaa gttcatcgag aacatgcgaa
    23341 agcggaatta gctgctttgc tgcgaatgaa cggctcttta ggtattgtga accagcagtt
    23401 tattttgaac gtccaaacag aaaatgctgc aattgccaga cgtatctatt ctcttttgaa
    23461 agatcattat caggtacgaa gtgagctact cgttcgtaaa aaaatgaaat taaaaaagaa
    23521 caatgtctat atcgtccggt tgaagcaagg cacaaaagat gtgttgatgg atcttgatat
    23581 catggatggt atgatgttca atggacacgt ttcggacgag attatgggaa attcgcaaaa
    23641 aatgcgttct tacttacgtg gtgcttttat ggcctcaggt tcagtcaata atccggaaac
    23701 gagccgttat catttggaaa tattttctat ttatgaacag cataaccaag acatttgcga
    23761 tatgttaaat tattacggtc tacatgctcg aacgcttgaa cggcgcaatg gctatatttc
    23821 ttatttaaaa ggagcagaaa aaattgctga tttcttaaca ttgattggtg caaccaactc
    23881 aatgctccga tttgaagatg ttcgaatcgt acgggatatg agaaattcgg tcaatcgtct
    23941 agtcaattgt gagacagcaa atctgaacaa gacgattgat gcggcttcta aacaaatcga
    24001 aaacatcgaa ttgatcgaag caaaggtcgg tctacatgcc cttcctgaaa aacttcagga
    24061 aatcgcagaa cttcggcttc aacatccaga ggtcagcttg aaagaattag gggaaatgat
    24121 tccctcaggt gcgatatcaa aatcgggaat taatcaccga attcgcaaaa taaatgaatt
    24181 tgcggaaaaa ctgcgtgaag aagcagttta atccaaagaa aaaactgata catgaagcag
    24241 gagatgcgaa tgtatcagtt ttttttaaaa gttatacttg cttttcttcc gtcaaactat
    24301 aattccatct ttactggagt aacataattt ggaaaggctt ctattgtttt taatacaatc
    24361 gtataaaaag cggggagaca actgaatgaa gattttagtt ttgtacataa atttttgctt
    24421 aatatagaag tcctgatata gataagatca agtatttatc tataactggc ctgattaatt
    24481 aaaaaactgg aaggaactgc tcgatcatcc ttgagaactg ctcttcttac ttctggtcaa
    24541 gattctcgtg gttggtggat aaggatggtt gataagttca ttggattagc tagtcgaaaa
    24601 caaccaggtg taattaatgt agaagtttta acaaggggat ggagtgattt attcagtgat
    24661 aatcataagt tcataaatag taattgagat gtatggttgg aatacgttat acctgaactt
    24721 gaactaccaa gatttggttt agaatttgag aatcgatgtt tataaaggga gggcaaaaat
    24781 gcgttttttt acaactttta ctatcattat gattgcagta ctttttattt ttctagatat
    24841 agcaaagcga aatacagcat ttttattata tcgggtttta cttcgagcag gtctcataac
    24901 atttatatcg atcgttggat tttttctatt cacagttatt gtatttattt ggagaactcc
    24961 agcaccacca ttgccagaga ttacatatgg agaatttcct tttagacttg agtatgaatt
    25021 gaatgaagaa ttacatgtga tagaagacac gttaatagtt gaatttgatg gatttggaat
    25081 gaacgaaggc atagggagat atcgtagatg gacaagtcgt ttagccagcg gagaagattt
    25141 agtgttgctg ttagaagtta gtgataataa gcaaatattt tattttccag gacctgctaa
    25201 ttattacatg ggagataggt tgaatggtta taatcatact tttcctagtg cttctttcat
    25261 tgaaagggaa agaggcatta ctagaaggga tattttacat gacaaagaat tattagaaca
    25321 atttggtccg cttgatcaaa atacaataaa tgaagaagaa ttgttaaatc aatataatat
    25381 aaggctagtg aattgggaaa tatctgaacc gattgttaac aattttggtg attagaaaac
    25441 aattacgata aatagaaaca agccaaatca tcagcaagta aaagctgatg atttggcctt
    25501 tcttatacga gtagtaggaa ttcatagcga atattattac gatcagtaga tattttgata
    25561 ttaaaggagt attacgaact agtatgtgag aatgtagagg tgatttgcga ataatgagaa
    25621 atttatcaaa tagaaacaaa atattaatca ttattgttgt tatagctgtg tttcatctgg
    25681 gaacgaatgc ggttttaagt agaataatat tgggtcctaa gccaccaaga cctgcgatta
    25741 cccgagggga gtttgatttt agattagaat atgaagtaga tggggaaagg attgtgatag
    25801 aagatacaat tgttgcatta tttgatggat tttctgcaga tgcaggtagc atggcatggt
    25861 atcgcacgtg gcgtttgcat ttggcgagtg acaagagggc acaacaatta ttgttacatg
    25921 agttagatga tggtcgaaaa atatattatc attttggagg agctggattt tatatgggga
    25981 agttagtagc agtgtaactg taacaaggaa agatttgcca ggtcttggca ttcgaaactc
    26041 catattttat atcgagcgtg ggagagatac atctattggg aacaggaaag ggttaactct
    26101 tgaagagtta tataatgact ttggaatccg tcttgttagt tgggaacaca atccaccaat
    26161 agaaaatcgc tttgaataat cgaaatctat taatccattt catcagccag tgaatgttgt
    26221 aatggaacaa tttggtttag tgttcaaaac cattgaaata ctaggctgtg atcagcaaaa
    26281 atgtatctga ataaaaaaga gagcaactat taaatgagag gagttaggag ttgttagctt
    26341 tatacgaatc aaagtggcaa atacagatgc gtatgcattt gtgtttgcca tcttgcgttt
    26401 ggtagacgag ataggtataa gctaaagaaa caacttgttt aaaataaacg acctagctca
    26461 acaaacgacc atctaatcaa ctaacagcat acataaaagc aactattttt gaagtagaat
    26521 tgaaatcggg ataacttaaa aagtgattgg gatactagtg tgaatcaccg tacataaata
    26581 ttactcgtta agggaatttg agaaattttg tatgaaaatt taagaattag ttagtatgcg
    26641 caagtcaaaa atatgaataa attatttggt ggagataatt tgctcagctt gtcataagaa
    26701 atgagataat ctggcagatg caagcttttg gacaccattt attcggaata aggaggctgg
    26761 gacagaagcg tttaactcca agaaataaga aggagttgct tctgcttccg ccgtttatag
    26821 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ctacgctcct tattttttta
    26881 ttttgtttgg atcaattctt ttaattcctc tattgaaata gattcatcaa agatttcttt
    26941 acccaagatg acagtcggaa caaatcgaat atgtgcttgc tcagcttcgt tgatgacagc
    27001 ttggctcagc tgttcgtctt tttgttctgt cagctttagt ttatctactg caaaatccgc
    27061 tacttcttgg agagaaagat gtttccactc gtcttgagta tcgaatattt gtttgatctc
    27121 tttgatcgct ttttgaccat cgctgattgt gagataccgg tgcatcacgt ttcctcgctg
    27181 caagctttct ttttctttat cgaacaattt gatcactcgc tgcaattgcc cgctttggac
    27241 tgcttcttct aatagctcat aagattcttc aaaccattgt cggcaatacg gacaagatag
    27301 gttgatgaat tcgaccattt ttttaggagc attcgatgaa ccataaagga ttcctttttg
    27361 tgtatttgtt ttcgttgcat caatcactga tatatccatg ttaaaacacc tctttcgctt
    27421 atagtttata catgtctgac aaaaaaaggc aacgatttag actgtttgtc taaaaattag
    27481 atgaccaatt caaatagaca tatgtgaaat atttttataa ataaaagaaa aaatggaatg
    27541 ttttacgtta taatggaaca gataataaga gagaatatga acaaggatga tgaatcatgc
    27601 gaaaaatgaa aacaatggat ggcaatacgg cagccgcata tatttcttat gcatttaccg
    27661 agattgctgc aatatatcca ataacaccca gttcgaccat ggctgaatta gtagatcaat
    27721 ggtcagcagc aggaaaagaa aatgtattcg gtcaaccagt aaaagtagta gaaatgcaat
    27781 ccgaagcagg agcagctggt gtggtccatg gatcattgaa aactggtgca ctgacaacta
    27841 catataccgc ttctcaaggg ctgcttttga tgataccgaa tatgtacaag attgctgggg
    27901 aactactgcc aagtgttttc catgttgcta gtcgggcagt gacgacaaat gcgttgaata
    27961 ttttcggaga tcacggagac gtgatggctg caagacaaac aggatttgct atgttagctg
    28021 aaagcagtgt acaagaagtg atggatttag cacctgttgc ccatcttgct tctattgaag
    28081 gagaagtacc ttttttgaat ttctttgatg gattccgtac gagtcacgag attcaaaaaa
    28141 tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa tcaggagaaa ttagcttcat
    28201 ttcgtaaacg agggatgaat cccaatcatc cgactgttag tggtacgaac cagaatccag
    28261 acatccattt tcaacaacgg gaaacgatca acccctatta tgataaatta cctgaaatcg
    28321 ttcaaaaata tatgaaagag atcaaccgat tacgaggaac ttcttatgac ttggtgactt
    28381 attacggagc agacgatgcg gaagaagtca ttgtttctat gggatcagtt gcacagacga
    28441 ttgaacaaac gattgattac ttgaatgcaa acggacggaa aacaggtttt ttgaatatcc
    28501 atctttatcg tccttttcca gtggaaacat tcgtgcaaaa gttgcctcat acagtaaaaa
    28561 caattgctgt tcttgatcga agtaaagaac caggggcagg tggagaacct ctcttattag
    28621 acgttcaaag tgcgttatat gattctgaac ttcgcccggc tgttattggt ggtcgctacg
    28681 gattggggtc taaagatgtg actcccgacc aaatctcagc agtttttgat gaattgaaaa
    28741 aagacccaag catacgcaaa aaacggttta ccatagggat cgtggatgat gtgacttatc
    28801 aatctttgga accaaaggaa agtctggatt tgacagaacc gcaaacattc caggccaagt
    28861 tttggggatt tggatctgac ggaacagttg gagcaaataa atcagctatc aagattattg
    28921 gagatcacac cgataaatat gcgcaaggct atttttatta tgattcgaaa aaatctggcg
    28981 gtctgactgt ctctcatctt cgttttggag atacgccgat acgttcagcg tatcttgtcg
    29041 agcatgcgga ccttgttgcc tgtcacacac cagcttacct gcatagctat gacttggtca
    29101 aaggattgaa gccaggtggc atttttttat tgaatactct ttggagcgac gaacaattag
    29161 aaacgcatct gcccttaaaa ttaaaacgtt acttggctga aaataacatt agattctata
    29221 cgatcaatgc catgagattg gcccaagaag tcggtttggg acgccggatc aatacggcaa
    29281 tggaaacggc tttttttaaa ttagctgaca tcattccttt tgatgaggtg cttccgctgc
    29341 taaaagaaga agccctgaaa agctatggac ataaatcaat ggctatcgta gaaaaaaaca
    29401 tacaagcaat cgatcggacg accgaactgc ttcatcaagt cgcgatccca gaccattgga
    29461 gaacgttgga gatgcctgaa aaaatcaata aaaggaacac tggttatata caagaaatac
    29521 ttgaaccagt gaatgcacag gaaggaaatc aactctctgt tggtacactt gtagcaaatg
    29581 gaatgacaac tggcgaaatg cctttaggaa cggcttctat ggaaaagcgg ggcgtcgctt
    29641 tagaagtacc tgagtggatt agtgatcgct gtacgatgtg taatgaatgt gcctttgttt
    29701 gtcctcatgc tgccatccgt ccatttttag cagatgaaga ggaaatggaa gaagcgccag
    29761 aaggatttat cgtgagagaa atgcgaggcg cagacggcgt aaaataccgt atccaagttt
    29821 cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc ttgtcccgca aaagaaaaag
    29881 ctttagtgat gaagccttat gaagaacaga aagaacaagc tgtgaattgg gcttttgcaa
    29941 tgacattaag acaaaaagca aatccagtca aaggaaagaa tactgtcttg tctacacaat
    30001 tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg ctgtggcgaa acaccttatg
    30061 taaaattatt gacacaaatg ttcggtgatc gaatgatgat cgcgaatgcg acaggctgtt
    30121 cttctatttg gggcggtgct tctcctgctt ctccttatac gaccaatgaa tgtggtcaag
    30181 ggcctgcatg gtcaaattct ttattggaag ataatgctga atttggttat ggtatgcttc
    30241 tagcggcaca gactcgtcgc gaggctttgg caagttcaat caaagaagta ttgccaaaag
    30301 tatcagaaga actacgagaa ttgatggaag actggttggt acacatgttt gagagcgaag
    30361 gaacgcagca aagagcagca aaactgcaag ctgcgatcga aagcgaacga tcaattgtcc
    30421 ctgaactgga ggatatatac aagagaaagg atctattcgt aaaaaacagc caatggatga
    30481 ttggtggaga cggatgggct tacgacattg gttttggcgg gatcgatcat gtacttgcca
    30541 gcggagcaga cgtgaatatt cttgttttgg ataacgaagt ctattccaat actggcggtc
    30601 aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt ttcggctagt ggaaagtatg
    30661 cttcaaagaa agatctagga atgatggcga tgacatatgg aaatgtttat gtggcgcaaa
    30721 ttgcttcagg tgccaatcaa atgcagacga taaaggcttt tgaagaagca gaaaagtatc
    30781 ctggaccttc tttaatcatt gcctatacgc cttgtatcac acatggtttg cttggtggaa
    30841 tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc tggctattgg tcattatatc
    30901 gatataaccc tgcaatggtc gaaaaaaaca agaacccaat gactttggac tacaaaaaac
    30961 ctgatttctc tgctatgcct gatttcatga ggaaacaagt tcgcttttcc tcgctagaga
    31021 acgcaaatcc aaatcttgca aaagctttat ttgaaaagac tatccaagac gctaaaacac
    31081 gtttttacaa ctatgctgca ctgacgggag aattagaaaa aatcaagaaa cgacttgaac
    31141 cagaggaatc tatcgatgta cccgttaaga ctagaaaaga aaaaacgacg gaccttgaag
    31201 cagaagaaag aagagctgcc agagcagaac gacgtaaaaa aatggaacaa gctacaaaag
    31261 aaaaaaagga aaaataacag aaaaaatata atgaataatt ctctgaagtt taaaaatatt
    31321 tattgcagag aaaaataaaa cgcatacttg caattttcct gaaaattatg cgtttttctc
    31381 aagaaaaata cgagcagatc agcctctaaa attaaaacat atgatatact agagaggaaa
    31441 tctcaaaaga gaagagaggc tttttatgtc atttcaaatc ggggaacttt ttaatttgga
    31501 ctattggcag caaatgtttt ccggtgactt gttctctctg aattttttgg tgaacatact
    31561 tgatattctg gttgtctggt atcttgtata caaactgatc caactgctgc gaggcacaaa
    31621 agcgattcag cttctgaaag gtgtagctat ttttattgtt atccgaatcg tagcggaaat
    31681 cattggtctg cacaccttgt cttggctgat gaaccaagtc attacgtacg gggtgatagc
    31741 tgctatcgtg atttttcagc cggaaatccg gagaggactg gaacatcttg gacgcagttc
    31801 gcttttcaaa caatcaaaaa gcgaacagca ggaagatgag aagatgatct tgtcttttga
    31861 caaagccatc caatatatgt ccaagcgaaa aatcggtgcg ctaattacta tcgaacggca
    31921 tactggatta gatgagtata ttgagacagg aattgcatta gatgcagata tcactggaga
    31981 gctgctgatc aatatcttta ttcctaatac accgctgcat gacggtgcag ttattgtaaa
    32041 agaaggaaag atcgcagtag caagcgccta tcttcctcta tcagaaagta tgttgattcc
    32101 aaaagaattc gggactcgac accgggcagc tgtcggtata agcgaagtca gcgacgcgat
    32161 taccatcgtt gtctctgaag aaactgggga tgtcagcatt acattagata atgaactgat
    32221 ggctggctta tcccagcaag aatatttggc aattttgcgt agagaactga ttgctgaaga
    32281 gaaaacaaaa aacaagaaaa acgtgattca atctttccta gacggtatga ctaagggagg
    32341 ggatcgcaaa tgatctcaaa agagaaacgg acaagcattc tttacagttt tattgcattg
    32401 ctgttcagta ttgcgctgta cttcaatgca aatggccagt ccgttccaaa tacgctttct
    32461 ggaaatgaag cgtataatca aacagttact ggggtgccaa tcaagatttc ttatgattca
    32521 aagcgctact atatccatgg atatgaaaat gcagtgactg tcaaactttc cagtgcaaac
    32581 cgagttcagt tgaatgcaga ggcaaacgaa gatacacgga tgttccgcgt cacagcagat
    32641 atcactaagc tgggcgaagg aacacatgag gttccgctaa aaattcaaaa tttgagcagt
    32701 gctgtcacag caaagatcga accatcgact attacagtaa cggttgagaa aaaggtaata
    32761 aaagatttcg atgtggaaac gttgattcct tcaacgttca caccatctgg atatgaactt
    32821 gatgatactt ccgttagtcc taaaaaagtc tctatcacta caggtgacaa aacattggca
    32881 gaaatcaaac gagtagtagc tgatgtggac gcttcgatgg tcacagatga tgggattaat
    32941 agtgaggtcc caatacaggc gctgaatgca gcaggggaag tattaagtat cgtctctgat
    33001 ccagtgcaag taacagtgag agctgattcg atcaagccaa gcaagtcggt acgactttat
    33061 gggatacagc aaggaacacc agctgccggg gtagaaagct atgatttcag tttttcaaaa
    33121 ctggaagcag aagtaagcgg ctctagcgat ttgcttgcat cgatcggtga cagtattgct
    33181 gtaccgatca atgtctcagg tatcaatcat cgaacaactc gtaaaattga aataccagta
    33241 gaagaggggt taagtatcaa gcccgaatca gttagtgtgg agattacacc cgtgttgcaa
    33301 acgtcaagca gtagctctgc ttcgacgagc agtcggacac aagaaaatac aagtacgagt
    33361 agaagtattt catctgctga ttcaggaacg gaaccatcga caacaaccat ttcttccagt
    33421 tcggaatctg ggacaagcag ctcgaccaca agtacagaag aaacatcaga aacaagcgaa
    33481 tcaacacaaa tgagcagaac aaattcctca gaaaattaag ttaaaggagt aattttacaa
    33541 ctatgggtaa atattttgga acagacggcg tacgcggtga agccaacaag gaacttacgc
    33601 cagaacttgc atttaagctg ggacggtgcg gaggttatgt cctaagccag catgaagaag
    33661 aaggaaaacg cccacgcgtg ttagtagggc gggatacaag aatctcaggt caaatgttag
    33721 aagaagcgct gatttcagga cttttatcgg tgggtatcga agtgttccag ctaggtgtga
    33781 tctctacacc aggtgttgct tatctgacac gtttgcaaaa agcaagcgca ggggtcatga
    33841 tttctgcttc tcataatcca gcacaagata atggaatcaa atttttcggt gcagatggtt
    33901 ttaaactagt agatgaccaa gagctagaaa ttgaagcatt attagatgct gaagtggatg
    33961 aattgcctcg tccatcagct gaaggattgg gaactgtaga agaatttcca gaaggcttat
    34021 tgaaatattc tcaattcctt gtacaaacga tcaacggtga cctttccggt ttgaccattt
    34081 gtgtcgatgc agcaaacgga gcaacttcaa caagtgttaa tcgtttgttt gccgatcttg
    34141 aaacagattt ttataccatg ggcacaaatc cagacgggtt aaatatcaat gatggcgtag
    34201 gctctaccca tccagaacgt ttagctgaga tggtcgttga aaaaggagca gatgctggac
    34261 tagctttcga tggcgatggc gatcgtatta ttgcggtgga tgaacttgga aacattgtcg
    34321 acggtgataa aatcatgttt atctgtgcta aatacttggc agaaaaaaat cgattgaaaa
    34381 aagatacgat cgttacgact gtcatgagta acttaggatt ccacaaagcg gtggaaggaa
    34441 tcggtttgaa agatgtcgtt acacaagttg gtgatcgtta cgttgtagaa gaaatgcgca
    34501 aaaacgatta taactttggc ggcgagcagt ctgggcacat gattttctta gattttaaca
    34561 caactggaga tgggatgctt tcaggaattc agttactgaa tatcatgaaa cagacaggta
    34621 agaaattatc agagcttgca gcagaggtaa caatctatcc gcaaaaatta gtgaatatcc
    34681 gagtaagtga taaaaacggt gcgatggatg ttccggcaat caaaaaagtg attgaagagg
    34741 cagaagctga aatgggatca gaaggaagaa tcctagttcg tccttccggt acagaaccat
    34801 tacttcgagt gatggcagaa gcgcctactg atgagaaagt taattactat gtcgataaaa
    34861 tcgcggcagt agtcaaagaa gaaatcggac tggattaaga gatattttca taaaaaataa
    34921 aagaacaagg accagtgtca gatgtctgac actggtcctt gttcttttat tattctactg
    34981 tcactgattt cgctaaatta cgtggtttgt caacgtcgaa tccccgtaat agcgttgcgt
    35041 aatatgcaag taattgagtc ggtacgactg tggttagagt agttagataa gggtggacag
    35101 ttggtagcac cagctggtca ctttcctttt ctaatccctc tgagacaata actagtgttt
    35161 tagcgccacg gctttcgact tctttcaggt ttcctcgagt atgcgcacca gtcacttctt
    35221 cactgatgat tccaatcact ggtgttcctt cttcgatcaa agcaatcgtt ccatgtttca
    35281 attcgccagc ggcaaaacct tctgcctgga tataactgat ttctttcaat ttcaaagcag
    35341 cttcaagaga aacgttgtag tcagcgccac gtccaatgta aaaggcattt cttgtatcgc
    35401 tgagatatac ttctgccaac tcttctaaat aatctttttg ggcgatgacc gattccatcg
    35461 cattagcaac taaactcaat tcatgagtga tatcgaatac aagagaagtt tcaaattctt
    35521 ttttatcacc gatagctttg gctaataaag ttagtacagc tatttgtgca gtataagctt
    35581 tcgttgatgc tactgcgatt tcgggtccag catgcaatag cagtgtaaag gatgcttccc
    35641 gcgaaagagt agaaccagca acatttgtaa tcgtaagtga agggtagccg agacggttga
    35701 tttttaccag aacttggcgg ctatctgctg tttcgccact ttgactcaag aaaataaaga
    35761 atggtttttt tgaaagaaga ggtgtgttat agccaaattc actggctaaa tgaacctcaa
    35821 cagggatctg tgtcaatttt tccagagtct gtttacctgc taaccccgca tggtaactag
    35881 ttccgcaagc aacgatatag attcgatcgc tatcagacaa tgtatcgacc agcttttgat
    35941 caagagagac acgattgtct tctgttacgt atttttgagc gatacgacgc atgataatag
    36001 gctgttcgtc gatttctttc agcatataaa aaggataggt acctttctcg atatctgaag
    36061 aatctacttg tgcttcgaat gaagaacgtt ctacgatgtc acctgcaaaa ttttgaataa
    36121 taactttttc tgatgtgacg gtaaccatct cttcatcttc aatctcaacg aattccttcg
    36181 tatgagcaag catagccatt gcatcgctgg caatcacatt gaatccatcg ccgagtccga
    36241 ttaatagtgg acttttattt ttagcgacgt aaataacatc aggtgctgtc cgatcaatca
    36301 aagcgaaagc gtaagaacct ttgatgaccc gcaaagcttt taaaaacgcc tcttgcgttg
    36361 tagtatcctt cgcgaaagtt tcaattagat gagcgataat ctcagtatcg gtctcaccaa
    36421 taaagtggtc atttgctaaa taagcaattt taagttcttc aaagttttcg attacgccat
    36481 tgtgaacgag cacgaagcgt ccactttggg aagtatgtgg atgggcgttt tcttcagaag
    36541 gttgcccgtg agttgcccag cgtgtatgac caatacctat atttcctgta gtatcaatat
    36601 ttattttgtt tttcaaattt tgaattctgc cttgtgcttt gaccagataa tcagttgtgc
    36661 catctgagac aaagatacca gctgaatcat aaccacggta ttctaatttt tctagaccgt
    36721 caatcaatcc tggtgttaca ttttttaatc caatcattcc aacaattccg cacataaaaa
    36781 acatcctact ttctataatg aattcgtatg aatgcttcca gctgcaggaa aatccttttg
    36841 ttttatcttt gcagatattt tttgtggatt ttctgtagag tgcagtccct agggccatcc
    36901 gccgaatctt tcgataaacc ctctcctcgt catctgttta cacagacctg gcgctatcct
    36961 aaaaggtata actatcctcc tcttttgttt tttatctgga aacatttaaa atcataacct
    37021 tttgcgaatg ttcttgtcaa caaaaaaaga ataaaaaata aattggtata tatcaaatgc
    37081 tttgtcaaat gctcttttta tgtcaaaaaa taatgggttt ttatctatca tctttttcag
    37141 gagttcggta caatagaaag taatcggata gaggaggagt aaaaatggat tcaaaaattg
    37201 agatcatgac actgggaatg ttaaaaaaac aattgtccaa attcgaagca tcagctggtg
    37261 tatcagatga tacgaaaata tttttagata ctggctggga tagcatccaa gaaatcgcac
    37321 ctgatgcctt ggaagttgct gaagctcgtg agtttacggt agaagatgaa ttgacaaaag
    37381 agtcgttttc cgggtatgcg cgggaagaaa aagcagagcg gttcgacact tctgagcagt
    37441 cagaaactgt gatcgtcata aaaaacttgt attagaaagg agaaagaatg aataagagac
    37501 gttgggcagc agttctgatt gctgcaggat tatttgtggt ttcgttagtt tcagctagtt
    37561 tgactaagcc tcaggaaaac gatcaaattg agggattgaa cagttggtta tatggggatg
    37621 aagaactagc ttcaaatgtg ttggaagagg gagacagtac gcaaaaaatc gtaaaattat
    37681 cagtagatgg aacgattgct gcgggagggt cttcaggttt atttgcttct gaaggttaca
    37741 atcataaaaa ttttatggaa caactgaagg cgattgaaga agatccaaca gtcaaaggta
    37801 tctttttaga agtaaattct cctggtggcg gtgtctacga aagtgcggaa atcgctaaga
    37861 aattggatac gattcgtaaa gaacatgata tcccaatgta tgtcagcatg aaaaacatgg
    37921 cagctagcgg tggctactat atttctgctc aagcagataa aatctttgca acagaagaaa
    37981 cagtgaccgg ctcaattggt gtgatcatgt caggattgaa ttattcagga cttctagaaa
    38041 aactgggtgt ggaagataca acagttaaaa gcggagcgtt gaaagatatg ggttcaggaa
    38101 cgcgaccaga gacaaaagaa gaagaagcgg tgcttcaagc atatatcgac agcgcatata
    38161 gtcgttttgt agcaatcgtc agcgagggac ggaataaatc tgaagaagaa gtcaaaaaaa
    38221 ttgctgatgg aaggatttat gatggtgtcc aagcaaaaga agtaggttta gtcgatgaat
    38281 tgggatttcc tgaagatgct ttaaaagcta tgcgaaagga acaacagctt gaagatgcgg
    38341 aactagtaga atattcttcc acgccatctg ggtttgctag tacatggtta ggaagtaaac
    38401 tagcagaatt ccagggattg aagccttctg agactagtca gatcttatcc gttcttgaga
    38461 atctagggac agcggaatca ccaaaagcaa tgtattatta cggaggtgag taagcatgtc
    38521 agagctttca aatgaaaata aggaacaaga aaaagataag caacagcagc ctgctgtagg
    38581 acctgactcc aacttttccc aaaaggtact gagctctttt gaaagagagc ctttgacgcc
    38641 cgaagaaatc aaaaagaaac aacgtaaatg gaaaaagtat acggacacaa gaaatgaacc
    38701 gccaaaagca aatgattttc ctaagtattt ctatgcaggt ttttttatcc gaatgtttgc
    38761 tttcttggtc gatcttttgt gtatcggagc tattacgcgt ttaacacttg gtattgccaa
    38821 aaatctaggt tggcttaatt tttcagattc ttatctgagt atctacggat tcacagcctt
    38881 acttatttat ctagcgtatt ttattttatt gacgaaattc aatcatggtc aaacgattgg
    38941 gaaaatgatc tttggtattc gagtcatcag ctttgatgaa gaagaactta gctggtcaac
    39001 cgttttagtt agagaaggtg cttgccggtt cattctaaaa tttcctttgc tgatgttagg
    39061 gtatcttcca gcaatcttta atcagaaaaa gcaacatcta ggcgattttt tcactaatac
    39121 aagtgttgtt acgatcaatc tgatcaaagc attcaaccag gaagtcaaca gctgatgagc
    39181 aataaaaagc agtaacagtt gaaagcacct tctaaaagtc agatctaaaa tctagctttt
    39241 agagggtgct tcagttattt ttaattgtca tgcttttttt gttttgctac tattgaacat
    39301 gagctgaaca tgagcaagag gaggacactg cgtgaagccg attgattttc cagaaaaata
    39361 tgagaattta atgcgagtag cacaacaagc actagcaaac cagcaatatc agcaggcaaa
    39421 agaattattt caacgagctt acgagttgaa agaaagcttt gaggctaata gcctgcttgt
    39481 tttttgtctt tacgagttgg acgagaaaaa agaagcattg aagcaagcac tcttgcatga
    39541 aaaacagtat cttcaaaatg aggagtttgc tgaattttat tttgacttgt taatcagtac
    39601 acaagatttt ttatatgcta gaaaattgat agcaagtaca gatttttatg aagctttcga
    39661 gcagcgggtc attgaaaaaa tccaatttgc agaagaatta tccggacaaa tggagaggca
    39721 gaagatcaag gcactccaca ataaatcgga ggaacttcct tcgttggaac ctgcacgtca
    39781 gcttgcgtct attgatgaga tcgaacagct tccttatcat gagtttatcc aaacagcgag
    39841 taaacttatt gtatcgccag aggtccatat tttagcaagg gcaaaattac tggagacact
    39901 taggcaattg aacgaatgcc atcctgtttt ctatttgacg attgaagaaa agttagtcaa
    39961 agtcatacca aaagatctgc caaagccgca gcagcaggca agttaccagc aattatgtgt
    40021 attttcagat cgttacggta atgaagatgc gctgcttagt tccgtgttaa atgaagaatt
    40081 cacgttgcaa tctgcaattg tttatcctgt ctatgataca tacatcgaag acccgaagcg
    40141 ctggtttcaa ctgactgtag aggcgtgtac aggaaaaacg atgtatgaag gtacagaaga
    40201 tgaaaaacaa gattttttaa aaaaaagaga aaaaattttg cagcaaatga tcttcttcca
    40261 ttagatttta tcagtgaaaa agataaaaat gttatgaaaa acaaggattc tatccattca
    40321 atatgtttac aatttgaaag ggttagtgta ctatttaaag gtatgcatga aacgtttttg
    40381 atgtataaaa tgaatttcta aactcggagg gaattaaatg actgcaacat ttgaaaaaaa
    40441 aggcacgaat gatggtgttt taaaatttac gattgcgcaa gacgaaatcc aaaaaggatt
    40501 aacaactgcg tttaataaag tgaaaaaaag cttgaacgta cctggattcc gtaaagggaa
    40561 agtttctcgt caagtattca accgtatgta cggtgaagaa gctttatatg aagatgcatt
    40621 gaatgctgta ttaccagaag cttatgaagc tgctgtcaaa gaagctggca tcgatcctgt
    40681 ttctcaacca aaaatcgatg ttgaaagcat ggaaaaaggt caagactggg taatcactgc
    40741 tgaagtaaca gtgaaaccag aagtaaaatt aggacaatat aaagatctag ttgttgaaaa
    40801 acaagaccgt gaagtaacag atgctgacgt ggatgcacgt atcaaacgtg aacaagaagc
    40861 acaagctgaa ttagttatca aagaagatgc tgcagctgaa aatggcgata cagtagtaat
    40921 cgacttcgaa ggctttgtag atggcgaagc gtttgatggc ggaaaaggcg aaaattactc
    40981 acttgaatta ggttcaggct cattcattcc aggttttgaa gatcaattag ttggtcataa
    41041 atcaggtgaa agtctagatg taaatgtaac tttcccagaa gattatcaag cagaagatct
    41101 tgctggaaaa gaagcagtct tcaaagtgac aatccatgaa gtaaaagcaa aagaagtgcc
    41161 tgaattagat gacgaattcg caaaagatgt tgatgacgaa gtatcaacat tagacgaatt
    41221 gaaagaaaaa tatcgcaaag aattagtaga aacaaaagaa aaagcagcgg acgatgcaaa
    41281 agatgaagca gcgatccgta tggctgttga aaacgcagaa atcgttgaat tgccacacgt
    41341 gatggttcat gatgaagtac atcgttcaat ggacgaattc ttgaacaaca tgcaacgtca
    41401 aggtatctct cctgaaatgt actatcaatt aactggttct acagaagaag acttgcataa
    41461 acaattcgaa ggtgaagcag aaatgcgcac aagaacaaat cttgtgatcg aagcaattgc
    41521 agctgctgaa aatcttcaag caacagaaga agaaatcaat gcagaaatcc aagaattatc
    41581 agaaacatac aacatgccaa tcgaacaaat caaacgcgtg ttgacagaag atatgctgga
    41641 acatgatgtg acaatgaaaa aagcaatcga attgatcaca ggtacagcga cagaagcgaa
    41701 agaatagaaa tcaagtaata aatcatttct gaagaagcta cttctagaaa attgtttatt
    41761 cgaaataagg gactgtgaca cgattttgtc acagttcctt attttttgtt aagagtaaaa
    41821 aatgcaaagg tataggtcag ttgatccttt gttaaacact ttttcttttt actaaactat
    41881 gttatgatag taaaagactg taaagtcttt gatagtttct tgcatacttt ttgcaagtta
    41941 aggggtgaag accatgtatg acaatccaag tagcaatgag accgtacgct gttctttttg
    42001 cgggaaaacc caagaagaag tgaaaaagat cgtagctggt ccaggggtct acatctgtaa
    42061 tgaatgtatc gacctatgta aagaaatcat tgatgaagaa ttttatgaag aagctgtacg
    42121 cgaattcact gaagtcccta aaccactaga aattcttgaa gtattgaata actacgtgat
    42181 tggacaagat cgcgcaaaac gctcgttagc tgttgccgta tacaatcact ataaacgtgt
    42241 caatcagcaa gcacaagaaa acacagatga tgttgaactg caaaaaagca atatctgctt
    42301 gatcggacct acagggtctg gtaaaacatt tttagcacaa acattagcaa aaacattgaa
    42361 tgtacctttt gcaatcgcag atgcgacaag tttgacagaa gctggttatg tgggggaaga
    42421 tgtagaaaac attttgctta aattgcttca atctgctgat tacaatgtgg aacgtgcaga
    42481 aaaagggatc atctatattg atgagatcga taaaattgcg cgtaaaagcg aaaatgtatc
    42541 tattactcgt gacgtttccg gcgaaggtgt ccaacaagcc ttgctgaaaa ttctagaagg
    42601 aactgttgca agcgtgccac ctcagggagg acgtaaacat ccacatcaag aatttattca
    42661 gattgatacg acgaacatat tgttcatcgt aggtggtgca tttgatggca tcgaaacgat
    42721 tgtgaagaat cgtatgggcg aaaagacgat tggatttggt acacaaaacc aaaaactttc
    42781 tgaagacgaa agtgtcatgc agcatatcat tcctgaagat ttactaaaat tcggattgat
    42841 ccctgagttt atcggacgat tacctgttat ggcagcttta gagaaattga caacagatga
    42901 tctggtacgg atcttgactg agccgaaaaa tgcgctagtc aaacaatatc aaaaacttct
    42961 ttctctagat aataccgaac ttgaattcga acctgaagcg ttgagagcca ttgcgaaaaa
    43021 agcaatcgaa cgcaataccg gtgctcgagg acttcgttcc atcattgaag aaatcatgat
    43081 ggatgtgatg tttgatatcc cttcaaatga aaacatcgaa aaagtgatta tcacaaaaga
    43141 agcagctgaa ttgtctggtg agccaacagt aatctataaa gattcagaga aaaaagcggg
    43201 ttgatgaaaa gaggctgccg taaaagtcat tttggctttt gcgacagccg cttgctttat
    43261 tcatcacctt gttaaggaga gagaccatga aagtccatca agctgaaatt atcataagtg
    43321 cagtggcacc aaaacaatat ccagatacac aattgccaga aatagctctg gcaggacgat
    43381 caaatgtagg gaaatcttct tttatcaaca cattgattga tcgaaagaat ttagcccgta
    43441 cttccggtaa gccaggaaag acccagacat tgaattttta tttgatcgag aacgcattac
    43501 attttgtcga tgttccggga tatgggtatg caaaagtatc caaaactgag cgagcaaaat
    43561 ggggaaagat gatcgagaca tatttaacga ctagagacca gctacgtgcg gtcgtttctt
    43621 tagtagattt gcgtcataag ccttctcaag aagatatcca gatgtatcaa tttttgaagt
    43681 attatgatat ccctgtaatt gttgttgcca ctaaggcaga taagatccct cgtggtaaat
    43741 ggaacaagca tgagagcgaa atcaaaaaag cgttggattt tgatcgcaat gacacgttta
    43801 ttctcttttc ttccgttaca aaacagggaa aagaagaagc gtgggcagca atcgaacaag
    43861 caattgaatc ataaaaagaa taaaaaagac ttaccagtca aactctttca taaaagtttg
    43921 gctggtaagt ctttttttga gcttaactta cttttgtttc ttgtcttttt tcttttcatt
    43981 ttgtggctgt ggttttagaa atcgatcgat ctcttctttt ggtgcaactg ttttttccgg
    44041 atcgacagca aatgattcga aaagtttatc taaattatct gtacgttcaa atttcaaccc
    44101 cattgtaaac acctc
//
LOCUS       DAFCLG010000009        41780 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFCLG010000009 DAFCLG010000000
VERSION     DAFCLG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.009"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            155..1414
                     /locus_tag="LND56_000514"
     CDS             155..1414
                     /locus_tag="LND56_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6742462.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1420..2001
                     /locus_tag="LND56_000515"
     CDS             1420..2001
                     /locus_tag="LND56_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBK6742463.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2083..2958)
                     /locus_tag="LND56_000516"
     CDS             complement(2083..2958)
                     /locus_tag="LND56_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6742464.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3106..3438
                     /locus_tag="LND56_000517"
     CDS             3106..3438
                     /locus_tag="LND56_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6742465.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3550..4758
                     /locus_tag="LND56_000518"
     CDS             3550..4758
                     /locus_tag="LND56_000518"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBK6742466.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            4912..5367
                     /locus_tag="LND56_000519"
     CDS             4912..5367
                     /locus_tag="LND56_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742467.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5729..6004
                     /locus_tag="LND56_000520"
     CDS             5729..6004
                     /locus_tag="LND56_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742468.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6017..8095
                     /locus_tag="LND56_000521"
     CDS             6017..8095
                     /locus_tag="LND56_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBK6742469.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8255..10141
                     /locus_tag="LND56_000522"
     CDS             8255..10141
                     /locus_tag="LND56_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6742470.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            10153..11100
                     /locus_tag="LND56_000523"
     CDS             10153..11100
                     /locus_tag="LND56_000523"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK6742471.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11120..11647
                     /locus_tag="LND56_000524"
     CDS             11120..11647
                     /locus_tag="LND56_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6742472.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11710..12363)
                     /locus_tag="LND56_000525"
     CDS             complement(11710..12363)
                     /locus_tag="LND56_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK6742473.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12495..13337
                     /locus_tag="LND56_000526"
     CDS             12495..13337
                     /locus_tag="LND56_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6742474.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13495..14391
                     /locus_tag="LND56_000527"
     CDS             13495..14391
                     /locus_tag="LND56_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6742475.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14394..15107
                     /locus_tag="LND56_000528"
     CDS             14394..15107
                     /locus_tag="LND56_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBK6742476.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15127..15645
                     /gene="msrA"
                     /locus_tag="LND56_000529"
     CDS             15127..15645
                     /gene="msrA"
                     /locus_tag="LND56_000529"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6742477.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15642..15869
                     /locus_tag="LND56_000530"
     CDS             15642..15869
                     /locus_tag="LND56_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBK6742478.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16021..16572
                     /gene="lepB"
                     /locus_tag="LND56_000531"
     CDS             16021..16572
                     /gene="lepB"
                     /locus_tag="LND56_000531"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6742479.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(16655..17155)
                     /locus_tag="LND56_000532"
     CDS             complement(16655..17155)
                     /locus_tag="LND56_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6742480.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(17434..17832)
                     /locus_tag="LND56_000533"
     CDS             complement(17434..17832)
                     /locus_tag="LND56_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6742481.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            18092..18952
                     /gene="ylqF"
                     /locus_tag="LND56_000534"
     CDS             18092..18952
                     /gene="ylqF"
                     /locus_tag="LND56_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBK6742482.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            18945..19712
                     /locus_tag="LND56_000535"
     CDS             18945..19712
                     /locus_tag="LND56_000535"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK6742483.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            19768..20625
                     /gene="dprA"
                     /locus_tag="LND56_000536"
     CDS             19768..20625
                     /gene="dprA"
                     /locus_tag="LND56_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK6742484.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            20747..22825
                     /gene="topA"
                     /locus_tag="LND56_000537"
     CDS             20747..22825
                     /gene="topA"
                     /locus_tag="LND56_000537"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK6742485.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            22794..24170
                     /gene="trmFO"
                     /locus_tag="LND56_000538"
     CDS             22794..24170
                     /gene="trmFO"
                     /locus_tag="LND56_000538"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBK6742486.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            24368..25273
                     /gene="xerC"
                     /locus_tag="LND56_000539"
     CDS             24368..25273
                     /gene="xerC"
                     /locus_tag="LND56_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK6742487.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            25310..25858
                     /gene="hslV"
                     /locus_tag="LND56_000540"
     CDS             25310..25858
                     /gene="hslV"
                     /locus_tag="LND56_000540"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBK6742488.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            25872..27272
                     /gene="hslU"
                     /locus_tag="LND56_000541"
     CDS             25872..27272
                     /gene="hslU"
                     /locus_tag="LND56_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBK6742489.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            27290..28081
                     /gene="codY"
                     /locus_tag="LND56_000542"
     CDS             27290..28081
                     /gene="codY"
                     /locus_tag="LND56_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBK6742490.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            28163..29038
                     /locus_tag="LND56_000543"
     CDS             28163..29038
                     /locus_tag="LND56_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK6742491.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(29170..29808)
                     /gene="plsY"
                     /locus_tag="LND56_000544"
     CDS             complement(29170..29808)
                     /gene="plsY"
                     /locus_tag="LND56_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK6742492.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            29935..30369
                     /locus_tag="LND56_000545"
     CDS             29935..30369
                     /locus_tag="LND56_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK6742493.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            30535..32580
                     /gene="parE"
                     /locus_tag="LND56_000546"
     CDS             30535..32580
                     /gene="parE"
                     /locus_tag="LND56_000546"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK6742494.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            32592..35042
                     /gene="parC"
                     /locus_tag="LND56_000547"
     CDS             32592..35042
                     /gene="parC"
                     /locus_tag="LND56_000547"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK6742495.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            35319..37550
                     /gene="pflB"
                     /locus_tag="LND56_000548"
     CDS             35319..37550
                     /gene="pflB"
                     /locus_tag="LND56_000548"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK6742496.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            37794..38555
                     /gene="pflA"
                     /locus_tag="LND56_000549"
     CDS             37794..38555
                     /gene="pflA"
                     /locus_tag="LND56_000549"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBK6742497.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            38640..39566
                     /locus_tag="LND56_000550"
     CDS             38640..39566
                     /locus_tag="LND56_000550"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBK6742498.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            39748..40641
                     /locus_tag="LND56_000551"
     CDS             39748..40641
                     /locus_tag="LND56_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBK6742499.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            40775..40900
                     /locus_tag="LND56_000552"
     CDS             40775..40900
                     /locus_tag="LND56_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742500.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(41034..41666)
                     /locus_tag="LND56_000553"
     CDS             complement(41034..41666)
                     /locus_tag="LND56_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742501.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        14665 a         7135 c         8768 g        11212 t
ORIGIN      
        1 gaaaaagttc tttttttggc agaaaaaagc aaaaaaatac ttgttttaga aaaaataggt
       61 ataatagacg gataccaaca tccaaacgaa aaagaattat tgagaacaat gacggtattt
      121 caacaaatcg tcagtgagat aagagggaat tgaaatgaat acaaatagcg aaaaaatgtt
      181 gcaagcttta tcagatgaag acttgtcaca agcacaaatc tttttagaaa aagcattaaa
      241 agaagaccca gcagatatat tagctgaact aggcgaagaa ttgctggcta ttggcttttt
      301 gatcgaagca aaacagattt ttgaacaact gatcaaccaa tacccagaaa atgacggact
      361 gaacatcccg ttagcagaaa ttgcaatcga agataatgag atcgacttag cttttgaata
      421 cctggaaaaa gtggataagc aaagtgatta ctatccgcaa agcttattgg cgatagctga
      481 tttgtaccaa gttatcggaa tccctgaagt gagtgaagca aaattgaaag aagcagctgg
      541 gttgcttcct gatgaaccat tgatccagtt tgcgctagct gaattgtatt tttcagttga
      601 tcgtttcaaa gaggcagcag tggtttatga aacattgttg gcaagcggga taaatgaaat
      661 ttcaggaatc tcgatgcagg aacgtttagg acaatcctta agtatgcaag gagaattcga
      721 agcagctatc gtacctttag aagaagcact gaaagaagaa aggacagatg atcgactgtt
      781 ccagctagct tttacgaatc ttcaattgaa agaaaatcag caggcaatca actatctgga
      841 agaacttcga gaagttaatc cgcattatca atcgttatat ctatatctag gacaagctct
      901 tcaagaagaa gaaatgattg aagaagcaca aacagtgtta gaagaaggaa tcaaagaaaa
      961 tccttaccaa gtcgaacttt atcacctggc ctcggaaaat gctttccgtc tgcatgatag
     1021 acagcgagca gaagaacttt tactgaaagc tttagaagta ggagacaaac aagatgaaac
     1081 attacttact ttaagtaatc tttacttgga tgatgaaaga tatgaagatg tgatacaagt
     1141 gatcaatcag atggaagaaa ctgctaatcc ttatgcagaa tggaacctag cccatgctta
     1201 taatgaatta gaagacttta ccgttgcagc tgtacactat gaacaggctt atcatgaact
     1261 atcacatgaa ccagatttct tgaaagagta tgcctttttc ttaagagaag aagggcagct
     1321 gaaaagagcg caagaattat tgacttatta tttaacactt gaaccaggag atatggaggc
     1381 actgtcatta ttagatgacc taacagagag gtgagaaaaa tgctaattga tgtacgggaa
     1441 aagaagaatt ttttaacgtg gatggtcaat catgtatctt ttagccgaag agaagttttt
     1501 tggatcttga attatcttgc caatcatgaa gcgattttaa gcaatgtcca ctttgttgaa
     1561 ggggcgaatt ttgcggatcg aggactgcag atcagtgatc tatctgttga aggggaaccc
     1621 atgaaattgt ttttgcaggg aaaagaattt acggacacag accagatttt tcatgaaatc
     1681 cgtttgaatt ggaaaaagcc tttatatgtg gaatgtatat tcgaaaatgc ttggcagaca
     1741 agagaatatc tatctatctt agaagacaat ccattttctt cttgggattc ttcagtcagt
     1801 gaagaagata ctgaattaat tgaacgatat tttcaagaaa aagaagaaga ggcaaaacta
     1861 aaaatgcttt atgctcaaat cgatcaagca ctagaagatg gagataggga tgcttttttg
     1921 aaattatctg atgaagttaa ccgaaagatc cttttgcaga cacaaaagaa aagtaaccgg
     1981 gtaagtgaac ataacaatta gttaaaaata gtatagtact aaaaaagaca aaggtcagct
     2041 ggatgtggaa tctttcctca tagcctatct ttgtcttttt tcttatagtt ttttcagtaa
     2101 tcttgtctgt ggcttcaaat aagtaaagcc ttgcccttcc acttcatgca cattgatgat
     2161 tgaaaggaaa gctttttcgt caatctcgtg gacgatcctc ttagcttcag ttaattcact
     2221 agagctaacg accatataga tgattttttt tgaattacca gagaatgcgc cttctccaga
     2281 aaggaaagtc acacctcttt gcagaccggt catcagtagt ggtgcaatct cttcgctttt
     2341 gttggagacg acaagtagac cttttgcaga atatccgcca tctaagatcg aatcaatcac
     2401 acgactgaaa acatacgaaa caatcaacgt atacatcata cgtttcaagt cgatataggt
     2461 taatgaagca agtaagacga tgatatcaaa tgccaatagg gaacgcccca tagtgatgcc
     2521 gtaattttgt tctaaaatcc gagcgataat atcactgccg cctgttgttc cacccatgcg
     2581 gtagacgatc ccgcttccta cacctgcaac taaacccgca agtagagaaa cgatcagtag
     2641 atcatgctgt aaattgattt ctagtggaac cctttgccag atccataaaa agaatgacaa
     2701 agaaagtgta cctagaacag tataggccaa ggaacgttta ccgaggatct ttcctcctag
     2761 taaaatcaat gggatgttga ttaataaagt cgtataagct ggatcaatac caaataaagc
     2821 tcttaagatc aaggtgatcc ctgagactcc cccttctgct aaatgattag cgatgttcaa
     2881 gtaaacaaga ccaaatgcat aaatacttgt tcctataatg ataaaaagaa cttcttttac
     2941 tgtttttcgt ttgaccatat gaattccagc tttcaattat gtattttcac agaatttcca
     3001 tttagaaatt ccttcctaaa tgtagcatgg aaattcgatt ttctcaattg ctcttatcta
     3061 ggatttgcgt taaagttttg gtaagatagt tttggaggga aaaagatgga taaaaaacgt
     3121 tctttataca gtatgcagca ggaagtggat gaatatatcc agcaattcaa ggtggggtat
     3181 ttctctccat tagctcagat ggctcgatta actgaagaag taggtgaatt ggcaagagaa
     3241 gtcaaccaca cttatggaga aaaaagcaaa aaggcttctg aaccagttaa ctcggtagca
     3301 gaagaacttg gtgatgtttt attcgttacg atgatcatgg ctaattcatt gaatatcgac
     3361 ttaactgaag tttttgaaaa aaacatggaa aaattcaatc aaagagacca taatcgcttt
     3421 gagcgtaagg acgagtaaac aagatgaagt ggtcctggcc ttgaagactc tgaaacggac
     3481 ttgtcaaaca gtcaccatgc aactgagtct ttatctcagg acaagaaaca cgcaggaaag
     3541 gttggaaaaa tgaaattaaa acaattacct ctggaatacc aaaaggcaat tccagttctg
     3601 aaaaaattag agaatgcagg atatgaagct tattttgtag gtggaagtgt tcgtgatatc
     3661 ttgctgaacc agccaatcca tgatgtggat atcgcaacaa gcgcatttcc agaagaaatc
     3721 aaacagcttt ttccaaaaac gatagatatt gggatcgaac atggaactgt gttggtatta
     3781 gaaaatgatg aacaatatga gatcaccaca tttcggacgg aatcaactta tcaggactac
     3841 cgtagaccag atcatgtcga attcgttcgt tcgttggcag aagatttgaa aagaagagat
     3901 tttacgatca atgcgtttgc tttgaaagaa gatggagaaa tcatcgattt gttcgatggt
     3961 ctgcaagatc tagaagaaca gatcttgcga gctgtaggca atcctcatga gcggttccat
     4021 gaagatgcct tacgaatgat gcgcgggttg cgttttgtca gtcaactagg gttcaaatta
     4081 gaggaagaaa ccttctacgc catcaaagaa aaccacgagc tattaggtaa aatatctgta
     4141 gaacgtataa atgtggaatt catcaagatg ctcttgggaa atgaacgggc cgcgggaatc
     4201 catgcattta ttgaaacaga gtgctacatt tgctgtcccg gtttgaaaga agaaggcgaa
     4261 gcacttttac gttttgctga attaccatcc gtcccattga aagaagagag ccaagcatgg
     4321 gcattattga tcagccaact ggatctaaaa gaaaatgaga ttcgttcctt tttgaaatcg
     4381 tggaagtgtt ctaaccaaat gattaaagac gttcaagcac ttgtccaagg actacgacaa
     4441 cgagaaactg gagctttgga accattcgaa ttgtacttct taggcaaaaa acatgcactg
     4501 caagtagaag aattgatgat ctattttgac gaaacacctc agatggatgc cgtaggagaa
     4561 tcctatgatc gtttacctat caagtcatta agcgaattag cagtagatgg taaaatcttg
     4621 ctagaagaaa ctggaaaaaa acctggtaag tgggttagtg aagctttgca attagctgaa
     4681 aaagccgtcg tagaacaaag aataaaaaat gataaaaaac aggttttgga ttttttatcg
     4741 aaagaaaaat tgatttaaaa aacactagac tgtatctgct cagctttgtt ggctacttaa
     4801 agtgggcaga tacgtcatta gaagaaaaaa cataagatgt gaccacttac ataaatttgt
     4861 gctaaactga ggtagtacaa gaataaatta catcataaag gagtcttttt aatgagtaaa
     4921 gaaatgacag cgctaaaatt ttattttaga aatggagaaa cctggacgat caaccgccgt
     4981 catatcggcg atctttggat caaacaaatc acaacaagtt ttggacgaat caatggcagt
     5041 gaatttgttg aaatccatcc gtgtgctgga tttaaaatcg aaatcttcca tgaaggggac
     5101 gcagttgcga cacatgatat caacctaggc ggactggaaa tgggcatgtt taatcgcgca
     5161 ttgaaatacg aagatatcga gcgaatggaa atcctttacc gaaatggcac acctgatctt
     5221 gtgtacttcc catatctaga taaaggaaca gaaggattag acaatcaata tcaatcaaca
     5281 aaaatcagcg aaaaaacagg taacttgtat atcgtgatca atccagatca acgtgtagaa
     5341 gatgtatacg gggaattttt cgaataaatc gatttttacg caaaaatgag gctgtgaaag
     5401 agatgtgtct tattactact gataggctgt ctcttccaca ccgtttattc agaataaggg
     5461 actgtgacaa gatttttgtc gcagccccat ttttttgttg tgataaaaga acaagaaagg
     5521 caggaatggg aaagtaaaca tccttttgtc agaaactttt atctttctta agttttactt
     5581 gatatctgat actatttgga atagaatgag gcgttgaaaa atttatggaa atttctaact
     5641 agcaataaac gatgaaacaa tgaaatattt ttcatccaaa aagaactttg ctataatgat
     5701 ggaagtttgt gtataggaga tctggaaaat gagagagagt tttgaacaac aaaaaaaact
     5761 gttgcatgat cgctatggcg ccctttcgat ggatgaccgc cgtcaaatac tttgtaagtt
     5821 gcgtaaaaga aatatattga tgtatcgtca gttggaacgt ctgaaacatg atctgcttcg
     5881 tttggagtca aaacgtgtac agtgtgaatt agaggggaac caaacacagg ttgaagtagt
     5941 cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa atgcttacac aaaataaaaa
     6001 ataaagggga tggacgatgg aatttgctga gttagttatt gtatttgcag taacgatcac
     6061 cttatcgaac atagctagcc gcattttgcc aatgattcca gctccactga tccaaatttt
     6121 ccttggagtg gtattaggtc tgacagagtg gggacaatca atcgattttg aacctgaatt
     6181 gttcttagtt atgattattg caccattgtt atttagagaa ggagaaaaag cagatatttc
     6241 ttcgattctc aaaaatttcg ggacaatttt gttcttggca tttggaggag tcattttgac
     6301 ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca agtgtcccac ttgctgcctg
     6361 ctttgctttt ggagcagcgt taggtccgac agatgcggta gcagtcagtt ctctttctgg
     6421 acgagtgaat atccctaaaa aggcgatgca tattttggaa ggagaaggtc ttttgaatga
     6481 tgcatctggt gttactgcct tccagtttgc tcttggtgcg ctgatcaccg gatctttttc
     6541 agcagtaaac gcggggatga gtctagtagt ttctagtatt gggggagctt tgatcggctt
     6601 tttgcttgtc tggttcaaac aaaaaataat tcatttgatc gaaaaagcat cagcacaaga
     6661 tgtcacaggg tatctgctga tcgaactttt gcttcctttt ctagcctatg tattggcaga
     6721 ggcatttgat gtatctggaa tcattgctgc agtggcagca ggtattctcc aagcttctgg
     6781 ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc ttgtcccaca gtacatggac
     6841 aactattgct tttacactga atgcattagt atttatattt ttaggaatcg aattgacgca
     6901 agtgttctcg ccagtatggg gagacggttt atatccaaac ggtcttttat tagcgattat
     6961 cgtattaatc tctgtcatgc tttttgtgat cagattcatt tctatcagtc ttttttatgt
     7021 attcaaagat ggcagtaaaa agttcaaaaa acagttgaac gagatcttga tcttaacatt
     7081 tggcggtgta aagggaacag tcagtttggc tacgattttt attttgccat tttcaatcaa
     7141 taatatgatg ttttaccagc ggtctttgct tttgtttctg acggcgggtg tcatcttagt
     7201 gactttggtc atagggatca ttgtcttgcc tatgctgacg gaaacagagg aagcgaaaag
     7261 cacggatttg aatgctttga tgattttaga agaggtcgtt gaagttttgc gaaaagaaat
     7321 aaaggaaatt gatcgtaata caaaagaatt tttagccact gaagcagtga tagaaaatta
     7381 tcaagaacgg atacgtgatc tgtacttgga agatttaacc gatgatgaaa agcaagaagt
     7441 acaagaaata caagcactga ttttgtcgat cgaacgggat ggattggatg aaagttatcg
     7501 ttcgggcaag ctttcttcta atggttatcg tttttactcc cgttttcttt ctcgattcga
     7561 acattctatc acaagccaga ttttgtcttt tatcggcttt tggttcattg ttgtccgtag
     7621 gttaatgcga atcgttctcc atccgaaaat gttttggcaa agaagatatg ctgatcgtca
     7681 gacatttatt agtgatgaag atatccaaga aatcagagag acatatcaaa aaaatactca
     7741 gttgattatt gaaagtttag ataacttgac tgatatttat gatgaaacga tggttcaatt
     7801 ttttatccag caacgcaaac ttgaaggaat aaaaatgaac tcggggaatt tgatttcttc
     7861 gtggatgatc cagcaagata gtctgtttac gaaaaaaatg ctaagaggtt attatcttga
     7921 aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc acgacgtttt ctgcgaacag
     7981 ctatcgcagg aatatcaatt tgttagaatc ttatacgatg aacaaaccat ctgataactt
     8041 ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg agtccaataa aataaaaagg
     8101 taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga atcggtcctg
     8161 ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga agttcactat
     8221 ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa aagtaactga
     8281 cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt tggtccacga
     8341 aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc tcttaaatat
     8401 tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc caactgatta
     8461 ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg ttctccaagc
     8521 agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg aagcattgat
     8581 cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta aagcagaaga
     8641 acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta ttttgcaaaa
     8701 attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc agaaaaaacg
     8761 agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag acgaaccaac
     8821 aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac aataccgtgg
     8881 cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa accgaatctt
     8941 cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt acgtcttaga
     9001 aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc ggctttataa
     9061 acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc agcaagcacg
     9121 gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag aagatgattt
     9181 agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca aagatggcag
     9241 ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc aatctcggga
     9301 aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta atattctagc
     9361 tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc atcttgcgta
     9421 ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt acttacaaga
     9481 agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag aattacttga
     9541 acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt caggcggaga
     9601 aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt tattattgga
     9661 tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt attttagaag
     9721 ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca aggtagcaga
     9781 taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa acatgtccag
     9841 ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac caaaaataca
     9901 gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag aatgggaaac
     9961 gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag aagagatgaa
    10021 tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat ctgaaacaga
    10081 agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg tagaagatta
    10141 ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg
    10201 catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata ccaaatgcgg
    10261 tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt tggattgatc
    10321 aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct attacaaaat
    10381 aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc agactataat
    10441 ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt caatgaagaa
    10501 tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc ctttgctgaa
    10561 acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga aacaaaagac
    10621 ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac tcctgattct
    10681 agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc tttgcctcct
    10741 tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca attgtatcaa
    10801 cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc gttgctgact
    10861 cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac attaggagat
    10921 gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg agaaatacgc
    10981 gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt tgagatggaa
    11041 gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat cgctgtttag
    11101 aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa acggattgat
    11161 tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct tcaaacaaat
    11221 gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg gtagccgtcc
    11281 gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag cagaaggcgt
    11341 gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag acggtatctt
    11401 tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta cgatattata
    11461 ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg ttgactggtc
    11521 tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc gttatccgca
    11581 tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac
    11641 gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc catagcttta
    11701 ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac ctaacataac
    11761 aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa agaatgcgcc
    11821 tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc caagagagtg
    11881 ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga tgatcgtaga
    11941 tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa tgccaagaat
    12001 cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga tactcagcaa
    12061 acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg
    12121 gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa
    12181 aagaatcatc atcgatccat agatagcata agagacaata tggagggagg aatgcaagtg
    12241 ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc caattccatg
    12301 ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt tcgttttttc
    12361 catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg tctgttatac
    12421 taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg ttaaagaaag
    12481 agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt acgatggaaa
    12541 aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg atcgacggaa
    12601 cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg atggcgaaag
    12661 caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg atgtatgatg
    12721 agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc ttgagtggaa
    12781 cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg gttatcgaca
    12841 gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa ttagcaaaag
    12901 aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac aatacaaaat
    12961 tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc agccgaacaa
    13021 ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc cattctgaat
    13081 taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt aataatttga
    13141 aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat gcggatgcaa
    13201 aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac atgcatatcc
    13261 ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg tttgcaatca
    13321 tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc ttgcagctta
    13381 ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata aagactacct
    13441 ttttgataaa atttttgata acattagaac caatcgaata caagaggtga aaaaatgaaa
    13501 ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat catcatctat
    13561 gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt aactactgtg
    13621 agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga ttcgctgact
    13681 gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc aaatgatcta
    13741 aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa tggtgaacga
    13801 agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa tatcaaaaca
    13861 gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat ccaaaataat
    13921 ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca ggaaaatgtc
    13981 acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta cattttaggc
    14041 atctacaatc cattttattt gaactttcct gagatcactg acatgcagac aattgttaac
    14101 aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta ctttatccca
    14161 atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga agaaggaaca
    14221 gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt gctctatgaa
    14281 gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc agtgagagat
    14341 cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata aacatgaaaa
    14401 caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa ttcgccttcc
    14461 ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt atttttgcta
    14521 atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga gatcctgtct
    14581 taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc cttagtgagt
    14641 atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca ctactcaatg
    14701 gtactttcga agtactaggt tttcctatcg atttctattt atattttgat ccgtatgtga
    14761 tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca ttgagtttgc
    14821 caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat tggatcgaag
    14881 tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg caaaacggga
    14941 tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt agtctatatt
    15001 tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact agcaccagca
    15061 gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat tagaggggga
    15121 atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat ccagcctttc
    15181 gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca tgtaccagat
    15241 ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt ggagattatc
    15301 tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca gcaaactgat
    15361 ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc ggtgatcttt
    15421 tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt agctgaaagt
    15481 ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt ttatctagca
    15541 gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag aaaccatgca
    15601 cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga agcttttttc
    15661 attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt ttcgctacag
    15721 aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa atttctaatt
    15781 atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa gcatgggaaa
    15841 aatatttaga aaataatcat gacatataag agatagtttt gtactttatg atcaaaaaag
    15901 ttttttgtcc aatagcctaa ttgaaagaat tatttttatt tcagctatct agtcgcattt
    15961 ttttgatatt tttcttataa taaagaaaga atgaaaaaaa gagggatgga ttgatagaga
    16021 ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg tattcaaata cttacttgtt
    16081 tctgtattcc ttgcgttcat gttgcgggga tttttattca tcccggtacc agtggaaggc
    16141 aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa attttcggag
    16201 atcagacgat ttgatattgt tgtttttcaa ttagcagatg gcacgattta tatcaaacga
    16261 gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg atcaattgaa gatcaatggg
    16321 aaagtagtta aggaacctta tctaactaaa aacctgaaat cagatcatgc aaatgcatcc
    16381 tatacgacgg actttacact acaagagcta acgggacaat ccaagcttcc ggaagataat
    16441 tattttgtac ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt tggcacgatc
    16501 aataaaactg atattctagg taaagcaaga tttgtctatt atccacttga tgaaataaaa
    16561 tggatccagt gagaaacaat aaagcgtgca gtctgttgag actgcacgct ttattgttta
    16621 atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta aacacttttc
    16681 tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg atttggatca
    16741 tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac aaaacagaca
    16801 tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg gcagcgatga
    16861 cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat ccaccaagga
    16921 aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg aaatttaaag
    16981 tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa cccataagcg
    17041 ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct atcttgtata
    17101 agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca ttcatttcgg
    17161 gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct ttgtcagtta
    17221 ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga tttacataca
    17281 ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaactg taaaaaagag
    17341 attgtacgaa taaaaaaaat agaggcggag aatacttatc gtatcatccg cctttgtttt
    17401 tttgttataa aaaacgtcag attagttatc tgcttacatc ttcatccata cgggttctaa
    17461 tgtatttccg tctagatctt ggacttctaa tgaaaacatt tcttcttcag gcattcccat
    17521 atctacttcg tggaaagtac caccattttc ttttgcaatt tgagcaaatt tttttacttc
    17581 atccgcattt tcaacactaa aagaaatcaa agcaccactt gtttttttat tatcagctat
    17641 ttctttatct tttataaatt ttttataaaa atcatgagag agaagcatta tccaaaaagt
    17701 gtcatcccat accattgaac cagcttcttc agtagaaaat tcttcatttt ttttaaaacc
    17761 cagcttttca taaaaagcaa cggaacgatt gacatcagca acaggaaaat taacaaaaac
    17821 catactattc atttgtttcg tctcctttct gttttgttcg tctatcatct cttatttagg
    17881 aaacgctgtc aaaaatttag attcatatat agaaaccaaa ataaaaattt gcatcatcaa
    17941 aaaagtgaag atgtgataga atgaggactg gttcactttc acttaactaa tagatggaat
    18001 ttctttataa taaaaaagtg aatttatatt gaaaaagaaa aaaacaagta aagccgggga
    18061 atattccggt atgaaaggaa tggagagaac aatgaccatc caatggtttc cgggacatat
    18121 ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat gtggacatcg tatttgaatt
    18181 ggtagacgca cgattacctt tatcttctcg taatccaatg ctagatcaga tcattcagca
    18241 aaaacctcgc cttgttttat tgaataaagc ggatctggca gatccacaac agacaaagct
    18301 gtggcaagaa tattttcgta aacaaggcca tcttgcatta gctatcacgg cacaagaaag
    18361 taaaggaatt aaaggtctag tacctgcggc taaagaagca ttgaaagaaa aacgggcaag
    18421 agatgctgct cgggggatca agcctcgcgc tattcgggca atggtcttag gtattccaaa
    18481 tgttgggaaa tctacgttga tgaatcgact agtaggcaag aaaattgcac agacaggaaa
    18541 taagcctggc gtaacaaaag gtcagcagtg gcttcgttta ggtaatgagt tggaattgtt
    18601 agacacacca gggattttat ggcccaaatt tgaagatgaa gaaatcggca aaaaactagc
    18661 gctgacaggc gcgatcaaag accagcttgt ccatttagat gatttagcga tttacggatt
    18721 agactttttt gcccgatatt attcaggaag aatcagcgat cgttatggtt tatcaatcga
    18781 gcaagaagaa aaattggcac cggaattact gatggatatt acagcaaaac gtgggtataa
    18841 agaagactat gatcgtggca gcgaattagt cgtgcatgat attcgtcaag gaaaacttgg
    18901 acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa agtgatgact gaatccatcc
    18961 aaagtatcaa agaacgattg aaaagcatac agagaataga tgatcctaca ctagaatcat
    19021 taagaaatga tccacgcaaa ggcgtgcagc aagcactggc ttcttttgaa cgacgattga
    19081 aaaaggaagc attaattaag gaaaaatatg aagaaatgtt ttgctttgaa aagcaagggt
    19141 acgcacaagg tcaccaatgg attgccggca ttgatgaagt aggaagagga ccattagctg
    19201 gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga gatcatatta gggctgaatg
    19261 actccaaaca attatcagag aaaaagcgag aacttcttta tcaacaagtt caagaaaaag
    19321 ccctggcaat cgggatcggg atcgttgatc agcagacaat cgatgatgtg aatatttatg
    19381 aagcaagtaa attagcgatg atccaagcag tagaaaaatt gtccattgta ccaagctatt
    19441 tgttgatcga tgcgatgacc ttggatttgc ctattcagca ggaaaaaata atcaaagggg
    19501 atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc taaagtatat cgagaccaat
    19561 tgatgaaaga atatgacgtg ctttatcctg gatatggttt ctcgcataat gcaggttatg
    19621 gaacaaagga acacttagaa ggacttgaac gacaaggaat tacgccaatc catcgtaaaa
    19681 gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc cgcttttacg tatcttatta
    19741 gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag aagaaaattt attgaaactt
    19801 gcttatacaa aaggaatcag tagtcaagga aaatgggcac ttgctaactg gatgatgcag
    19861 tatccttata aagaaatcgg atttgaagac atcattaaga taggtaaaat aaccaaacac
    19921 cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta actggggcaa aatccaatcg
    19981 aaacagtcgt ttatcacttg ctttgatccc aaatatcccc ctcagctgct tcacttgacc
    20041 tatccgccaa ttgtactgtt ttataatggt gatctatctt tactttcttg taacatgtta
    20101 agcgttgttg gtggaagaca cacttctgct ttagccaaaa aaacagttta ttatttacta
    20161 aaaccagtga tagaagctgg atatgtgatc gtcagtggct gcgcgaaagg gatagataca
    20221 tacggtcacc aagcagcaat ctctcatggt ggacggacaa tcgcagtcat tggaacaggg
    20281 atagagcaag tctatcctgc tgaaaatcgt gacttgcaag aggtgattgc aaatgatcat
    20341 cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa agcattgttt tccaatgaga
    20401 aatcggatta tcgcagcttt gtctaaagga acatgtgtct ttgaagccaa gaaaaaaagc
    20461 ggctctctga taacagcaca gcaagcattg gatcttggaa gaacagtttt ttctgttcca
    20521 ggaaatattt taacagaaca ttctaccgga tgtcatcatt tgatccaaga cggtgcagtt
    20581 tgcacggttt cgggtgaaga tatcttggca gaatttaaag aataacacca tacttttcat
    20641 ttgcttttct tgacaaacgt tttctgaatg gatatgatta gctacgattt gtatgaatcc
    20701 tcttttaata gtaggaacac gacaatggaa aggagtccat tcagtaatgg cgtataaata
    20761 tttagtgatt gtagaatcac ctgcaaaagc caagacaatt gagaaatatc ttggacgaaa
    20821 ctataaagta atggccagtg ttggccatat aagagattta ccgaaaagta aaatgggtat
    20881 cgattttgaa aacaattatg aaccccatta tatttctata cgcggaaaag gcgatgtcat
    20941 caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt tacttggcaa gtgacccgga
    21001 tagagaagga gaagcgattg cttggcattt agcgtaccta cttgggttgg atctgaaaga
    21061 aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca gtcaaagcag cttttaagga
    21121 accaagaacg atcgatgtag atttagtaga tgcacagcaa gctcgtcgta ccttagacag
    21181 aatcgttggt tattcgatca gtcctattct ctggcgtaag gtcaagaaag ggttaagtgc
    21241 aggacgtgtc caatctgtcg ctttaaaaat tattattgac cgtgaaaaag agatccgaga
    21301 atttgttcca gaagaatatt ggagcatcga cggtaatttt aaaaaagctc gcaagaaatt
    21361 caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa ttaccagatg cacaaagtgt
    21421 aaaagaagtc actgctagaa ttgatggaac agacttcgaa gttaaaaaag tagaaaagaa
    21481 agagcgcaaa agaaaccctg cgaatccttt cactacaagt agcctgcaac aagaagctgc
    21541 ccgtaagtta aactttcgaa caagaaaaac catgatggta gcacagcaat tatatgaagg
    21601 aatcactttg ggtaaacaag gaacagtcgg cttgattaca tacatgcgta ctgattctac
    21661 ccggattgcc gatacagcaa aagcagaggc cgcagcattt attgaagata cgtatggaaa
    21721 agaattttct agacatgatc accgaaagat caaaaatgct caaggcgcac aagatgccca
    21781 tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat gaactcaaac aatatttaga
    21841 taaagatcaa ttgaaacttt atacattgat ctggtcacgt tttgtggcta gtcagatgac
    21901 accagctatt ttagatacaa tgaaagtgac attagaacaa aatggtgtca tcttcattgc
    21961 gaacggttca aaaatcaaat tcaaaggatt catgcaagtg tatgtggaag gtagagatga
    22021 cggcaaagaa gagaaagaaa atattcttcc agaacttgaa gaaggcgatg tagtccaatc
    22081 tgttgatatt gaaccaaaac aacattttac tcaaccacca gcacgtttta gtgaggctac
    22141 gttgattcgt gcactagaag aaaacggggt agggcgacct tccacttatg caccaacact
    22201 tgatacgatc caaagacgct actatgtaaa attgacacaa aaaagatttg agccgactga
    22261 actaggagaa attgtcaatt cattaatctg tgaattcttc cctcaaatcg tcgatatcca
    22321 tttcacagca gaaatggaag gagatttgga taaaattgaa gaaggcacgg aagcgtgggt
    22381 caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa ctgacgaatg cggaagaaaa
    22441 aatcgaaaaa attcaaatca aagatgaacc agcaggattc gattgtgatg tctgtggaca
    22501 tccgatggtg ataaaattag ggaaatatgg gaagttttat gcttgcagta attttcctga
    22561 ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg acttgcccag tatgtcatga
    22621 aggacaagtc attgaacgaa aatctaagaa aaacagacta ttctatggtt gttcccgttt
    22681 cccagaatgt gaattcactt cttgggataa accagtgggc agaaattgtc caaaatgtga
    22741 cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa gtagtctgtg taaatggaga
    22801 ttacgaagaa gacatccaaa aataatagaa aagaggttct ttcattgaaa atggtaaatg
    22861 ttgttggagc agggcttgcc ggaagtgaag ctgcttggca gatcgcacaa gcaggagtcc
    22921 ctgtccgttt atatgaaatg cgtccagtca aatcgactga ggcgcatcac acaagtaatt
    22981 ttgctgaact agtatgttcc aattcattaa gaggaaacag tttggctaat gcagtagggt
    23041 tattgaaaga agaaatgcgc cgattgaatt cggtagtaat cacttcggca gatgaaacag
    23101 cagtgccagc tggtggtgcc ttagctgttg accgcgattc tttttctgaa acaattaccc
    23161 gaaaagtcaa agagcatcca ttagtaacag tgataaacga agaaattact gaaataccgg
    23221 aaggaatcac cgtagtagca acaggacctt tgacttctca tccattagca gaaagcatca
    23281 aagcattcaa tggttcagat ggtttttatt tctatgatgc agctgcacca atcgtagaca
    23341 aagctacgat cgatatggat aaagtatatt tgaagtcccg ctatgacaaa ggagaagcag
    23401 cttacttgaa ctgtccgatg acagaagagg aattcaaagc tttccatgag gcactaatca
    23461 atgccgaagt ggcagaactt aaatcttttg agaaagaaaa atattttgaa ggatgtatgc
    23521 caattgaagt aatggcacag cgaggattca aaacgatgct tttcggacct atgaaaccag
    23581 ttggattgga agatcctaaa actggcaaac gtccttatgc tgttatccag ctgcgtcaag
    23641 acaatgctgc agcttcttta tacaacatcg tgggctttca gactcacttg aaatggggcg
    23701 agcaaaaacg tgttttccgg atgattcctg ggttagaaaa tgctgaattc gttcgctatg
    23761 gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt gctgaaaccg acttaccaat
    23821 caacgaaacg cgaagatctg ttctttgcag gtcagatgac tggtgtagaa ggctatgttg
    23881 aaagtgcagc gagcggacta cttgctggaa tcaacgccgc acgcttagca aaagaaatgg
    23941 aaccagtcga gtttccacaa gaaacagcta ttggaagtat ggcttactac attactcatg
    24001 cggaaggaaa acatttccaa ccgatgaatg ccaactatgg tctgtttcct gaactgccag
    24061 agcgcattcg agacaaaaaa gcaagatatg aagcaattgc acaacgagct ttaaaagcta
    24121 atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc aaatgagtaa ggaaactcca
    24181 tgaagagggt gagacggata gtctcgctct ttttcttgcg tttttcttaa gaatttctca
    24241 aacaaaatgt gaggaattgc gccaattgtg aacaaactta gaaatattgg ttaaattctg
    24301 acaatttatt tgtcttttta taaacgctgt gctaaattat tctctgggac aaaaggaagg
    24361 tgagaaaatg acagaagcca cgtgggaaca aaaatttttg cgttatttga ttgtcgaaag
    24421 aggatattca gaaaaaacga aagaagcata tgaagaagac attctccatt tcaaacattt
    24481 tttggaggaa tctggagacg ctgatctgct gaaggcggaa tattttacgg ttcgtgtcta
    24541 tttgagtgca ttagctgatc atcgtcctag ctatagcaga aattctatca gtcgcaagat
    24601 agccagccta cgttcgtttt atcagtatct tctcaaagaa gaagtgataa aagaaaatcc
    24661 attttcttac gttcatctca aaaagaaaaa tttgagactg ccacgttttt tttatgaaaa
    24721 tgaaatgcaa gcactttttg acagcgtagc aggagatagt cctttggatt tgaggaaccg
    24781 cgctttactg gaagtccttt acgggtcagg catccgttta agtgagtgta gcagtttgtc
    24841 ggtatcagac attgattttg attcagaggt catgttgatc catggtaaag ggaacaaaga
    24901 gagatatgct cctctaggtt cttttgctca agatgctctg caagaatatt tcagggaagg
    24961 ccgccaggta ttgatgacaa aatatcacga agagcatgat atcgtttttg tgaatcatca
    25021 tggaaaacca atcacgccta ctggaattga atatgtcttg aatcaaatga tcaaaaaaag
    25081 cagtttggat agtgatatcc atcctcacat gctgcgccat acatttgcta ctcatttatt
    25141 aaataatggg gcagatttgc ggacagtcca agaattgcta ggacatgcca atttatcgac
    25201 tacccagatc tatgcccatg taacaaaaga aagtttacaa aaaaactatc gttctttcca
    25261 tccaagagcg taaaaataag gaaaagaaca aatttaggag gaaacaaaca tggtcgaatc
    25321 accatttcat tcaaccacta tctgtgccgt cgaaaaagac gggaaatttg ctatggctgg
    25381 agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa ggcactgcaa agaaagtacg
    25441 acgtatctac aatggagaag ttgttgtagg tttcgccgga agtgttgcag atgcgtttac
    25501 tttagaagaa aaatttgaag gcaaactaaa cgaatataac gggaatctac aacgcgcagc
    25561 tgtcgaatta gctcaagaat ggcggacaca gcaatctatg caaaaacttg aagcgatgct
    25621 gatcgtaatg aatgataaag aaatgcttct tgtttcaggt acaggagaag ttatcacacc
    25681 agatgatggt atcttagcta tcggatcagg agggaatttt gcgttggctg cagcccgagc
    25741 aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa attgcagaaa atgcgttaaa
    25801 tattgccgca gatatctgta tctttactaa tcataatatt atcgtagaag aactgtaaga
    25861 ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca aatcgtaagt gaattagatg
    25921 aatatatcgt aggacagcac gcagcaaaaa aatctgttgc cgttgctttg cgtaaccgct
    25981 atcgccgttt gcagttagat gaaaaaatgc aacaagatat tacaccaaaa aatatgctga
    26041 tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg acgtttggca aaaatcgtca
    26101 atgcaccatt tgtcaaagtc gaagcaacaa aatttactga agtcggttat gtcggaagag
    26161 atgtagaatc aatggttcgg gatttagtag aaaatgcgat ccaaatcgta gaaaaagagc
    26221 agtacagccg tgtatacaca agtgctaaaa aaaatgcaga aaaacgtttg gtaaaaatct
    26281 tggtacctgg tatcaaaaaa gaacagaaac aatccactaa tcaatttgaa caaatgatga
    26341 atctttttaa tgctagtcag caaaataccg ctgaaccgca agaagaagtg acagaagata
    26401 ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa gggtttactg gatagtcgtg
    26461 aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc aatgatgaac aatggattgg
    26521 agcaaatggg gatcgaccta aatgaaactc taggtgcttt gacaccgaag aaaaaagtgg
    26581 aacggacggt aacagtcaaa gaagctttag agttattgat caaggaagaa tcagcaaaat
    26641 tagtcaagga tgctgatatc catagtgagg caatcaaatt agcggaagaa agtggaatta
    26701 tttttatcga tgaaatcgat aagatcactt ctaaatcaca gcaatctgga gaagtatcca
    26761 gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg ttcacaagta aacacaaaat
    26821 acgggatcat ccaaacagat cacgtcctat tcattgcttc aggagctttc catttgtcta
    26881 aaccaagtga tctaattcca gaacttcaag gacgcttccc gatacgggta gaattagatg
    26941 atttgacagc tgaagatttt gtgaagattc tgaccgaacc aaataatgct ttagtaaaac
    27001 aatacgtcgc gttgatcggt acagaaaatg tgtcagttac gtttacaaaa gaagcaattg
    27061 aacggatcgc tcaaatcgct ttcgatgtca accgtgacac agataatatc ggcgcacgca
    27121 gactgcatac cattttggaa aaactactag aagatctatt atttgaagca ccagatatgc
    27181 aaatgggaga aataacgatc acagaagcat atgtggatga caaacttggc tcaattgcgg
    27241 caaatgaaga tttaagtcgc tatattttat aaaaaatcgg aggacgacaa tgacagactt
    27301 gctaacgaaa acccgcaaga taaacacact tttgcagcaa aagaatactt ttgacatgca
    27361 agcagatttg ccttacgata aaatggctgt cacactaggt ggtattcttg acagcaacac
    27421 ctatatcata ggaagtgacg gagtattact aggttataat gaacgtcacg atgtgaacaa
    27481 tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct gaatcttata ctgatacagt
    27541 ggcacagttg atgaaaactg aagctaatat cccaattact agtgatatta ctgcatttcc
    27601 ggtagagtca agagagaaat atccatttgg tctgacgact gtcgttccga tctttggagc
    27661 tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa caatcgtttg cagatgacga
    27721 tctagtctta gcagaatatg gagctacggt tgtcggtatg cagatccttt atcagaagtc
    27781 tcgaagcatc gaagcagatg tacggagtgc aacggctgtc caaatggcaa tcaacacatt
    27841 atcttatagc gaattgaagg ctgtacaagc tatctttgaa gcattggatg gagatgaagg
    27901 tcgtctgact gcttctaata ttgccgatag tatcggcatc actcgttctg tgatcgtcaa
    27961 tgctttacga aaattggaat cagctggaat cattgaatct cgttcgttag gcatgaaggg
    28021 aacttattta aaagtattga attcccgttt taaagacgag ttagcaaaac acagttatta
    28081 gaagtaaggg gactggggca gatagaaatg tttgttccag ccttcccatt ttaaaaagta
    28141 agtcgaagtt ggaggaatgg caatgactgt tattattcaa aatgaccagc tgatcgccga
    28201 aatcgctgaa cacggtgcgg aattgatcag tctgaaatca aaagaacatg gtttagagta
    28261 tatctggcaa ggtgatccag cgtattgggg cagacatgca ccggttttat tcccttttgt
    28321 tggacgttta aaagatgatc agtatgttta tcaagggaaa acatatcaaa tggggcaaca
    28381 cggatttgct cgggatatgg attttgaagt cattgaacat ggacaagaaa tggctagttt
    28441 catcttaagg agtacgccgg agacattaga aaagtatcca tttgattttg aattagtgat
    28501 cagctatgaa ttaggaggag acggcatcac ggttcattat caagtggcaa ataccggtga
    28561 aaaagaaatg ttcttttcta tcggtggaca tcctgctttc aatgtacctc ttgaagaagg
    28621 attgtcattt gaagatttct atctggcatt ttctccattg aaatcaagat tacgcctacc
    28681 attgaaaggc ccattcatcg atatggatca aaagacgtta ggtcagacaa atacgaatct
    28741 ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt tttgaaacaa ggggtctaaa
    28801 tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta actttaagct ataaagatat
    28861 gccttatgta gggatctggt ctccttatcc aaaagaagcg cctttcgttt gtatcgaacc
    28921 gtggtgcgga atcgctgaca cggtgaacag tagtggaaac ttgatggaaa aagtagggat
    28981 caatcaacta ggtgcccatg aaatcttcaa gacaaaatat tccatcacag tgaagtaaga
    29041 ggttgtgacc gagaagttag cagggaacac cgtttaccaa ggtgtgtaaa aaagctatcc
    29101 tgtaagtaat aagggagtgt gacatcgatt tgtcacactc ccgttttatc tctttggaat
    29161 gaaaaagtat caggattttt tcttgctgct attcaaacca aacgggattc gactttcagt
    29221 atggtttctg atacgcttga tattgtcttt atgccgataa aagataaagg aagaaactaa
    29281 aaaggcaatg atcgtcagta accaattggg cttagccaaa atcacaggag ctacataagg
    29341 aaggacgatc gtagataaag tgatcaatac tgcactgatc atactgctca aactcaccat
    29401 acttgtacaa aataaactaa cgataaagat gatacaagaa taaacaaaaa atacaggatt
    29461 aaatcctaaa agcattcctg cacttgtcgc aacagctttt cctcctttga acccagcaaa
    29521 aatcggaaat gtatggccaa gaatggcgca taccccaaac caaagagggt taatcccaga
    29581 aaggtggaag atcatcggca aacaagtagc caatgtacct tttaaaatat ccataagcag
    29641 gacaacaatc ccggctgttt tgcctaatac acgaaatgta ttggtcgttc cagtattacc
    29701 gctcccgtgt tgtcggatat cttttttgaa aaacaatttg ccaatccaga caccggaagg
    29761 aatagaacct aaaagatatg ctaaaataag taataagatc aatttcattt tttaccctct
    29821 ttcaagttga aaactatcct ttattttagc atgaataggt aacaggaaca attgaaaaaa
    29881 tgaaagatac aagtataata gatggaataa aaagaaaagc aaggggtggc agaaatgtct
    29941 gtaaaaaatc caacacaaga aacgattttc aactatttgg aagaagcaaa aaatattgcc
    30001 gtcgtaggct tgagcaataa accagaacga acaagttaca tggttgcaca ggtattgcaa
    30061 gacaatggat ataaaatcat accagtaaat ccattattgg ctggtgaaga aattctaggc
    30121 gagaaagtct atgcgaattt gacagaaatc gatgatccaa tcgatatcgt agatgtattt
    30181 agacggagtg aatttttacc tgaaatagca ggtgattttt tgaaaactga tgctaaagtg
    30241 ttctgggcac aattagggat tgaaaacaaa gaagcagctg agatgctaag aaaagcagga
    30301 cgtacagata tcgtaatgga tcgttgtatt aagattgaac ttggtaagtt gaaagaaaac
    30361 caaaaataaa aatgaccaca aagaagagat gatcgataga ggggaatgtc gaaaaagcgg
    30421 ttaggagata attaaaattg atttctcgtc taccctcttt tttttcacga ccttttctag
    30481 tatgataatc gtggtgcggt tttgccgcaa agtattattg aaggagcgtt tgccttggca
    30541 aaaaaagtaa acaacgaata caatgactcg tccatccaag tattggaagg attagaggca
    30601 gttagaaaaa gaccagggat gtacattgga tccactgaca gccgaggctt gcatcattta
    30661 gtctatgaaa tcgtcgataa tgcagttgac gaagcattat caggttatgg cagtgagatt
    30721 gatgtgacca tccatgaaga caatagtata actgttgcag atagcggacg tgggatgcct
    30781 gtaggaatgc atgcttccgg aataccaaca gttgaagtga tttttactgt gctgcatgct
    30841 ggcggaaagt ttggtcaagg agggtacaaa acttctggtg gactgcacgg tgtaggagcc
    30901 agtgtggtaa atgccttatc gaaatggcta attgtaacga tcgttagaga tggcgtagag
    30961 tatcagcaga aatttaaaaa cggcggtaaa cctgacggta ctttgaaaaa gataggaaaa
    31021 acaaaaaaag caaacgggac gactgtccat tttttaccag atgatacaat attcagtaca
    31081 acgaaatttt catatgaaat tttagccgaa cggttaagag aatctgcctt tcttctaaaa
    31141 ggcgttaaaa tctctctttc tgatcttcgt ggagaagaac cagtcaagga aatttttcat
    31201 tacgaagaag gaatcaaaga atttgttgat tatctgaatg aagaaaaaga tacattgaca
    31261 ccagttgttt atttttcagg agaaaaagaa ggtatcgaag tagaggtcgc ttatcaatac
    31321 aatgacggtt attcagaaaa tgttttatct tttgtcaata acgttcggac aaaagatggg
    31381 ggaacccatg aagctggtat gaaagcggcg atgacgaaat cttataatga atatgcgaga
    31441 aaagttggtt tgctgaaaga acgggacaag aatctggaag gtagtgattt cagagaaggg
    31501 ttagctgctg tactttctat ccgtgtacct gaaaatcttc tgcaatttga agggcaaaca
    31561 aaagaaaaac ttggtacgcc agttgctcgt accgtagtag ataacgtcat cagtgaacaa
    31621 atgggctttt atttgcagga aaatagcgaa atgagccaga tgttggtccg taaagcaatc
    31681 aaagccagag aagcgagaga agcagcacgt aaagccagag aagaaagccg aaatgggaag
    31741 aagcgtaaaa aaggcgaatc tcttctttca ggaaaattga cacccgcaca atcccgaaac
    31801 cctaagaaaa atgaattgta tttagtcgaa ggggattctg ctggcggatc tgctaaacaa
    31861 ggaagagaca gaaagttcca agcaattcta cctttacgag gaaaagtcat caataccgaa
    31921 aaagctaaaa tgcaagatat cttgaaaaat gaagaaatca atacgatgat ctatacgatt
    31981 ggtgcaggag tcggtcctga gttttctatc gaagactgta attacgacaa agtaatcatc
    32041 atgaccgatg cggatacaga cggcgcccat attcaagtat tactgcttac tttcttttat
    32101 cgttatatga aaccgctgat tgaagctggg aaagtatata tcgcgttacc gcctttatat
    32161 aaagtatcca aaggacaagg aaagaagcaa gtcattgaat atgcgtggac agatgacgaa
    32221 ttggcagcaa tgattaaaaa agtcggcaaa ggatatatgc tgcagcgata taaaggactt
    32281 ggagagatga atgcggaaca attgtgggaa acaacgatgg atccgacttc ccgtacgttg
    32341 atccgcgtcc gtatcgatga tgctgcgcaa gctgaacgtc gagtaaccac actgatgggt
    32401 gacaaagtag aacctcgaag aaaatggatc gaaaatcacg tgcaatttac gctcgaagaa
    32461 gatggcagta tcttagataa aaaagaggat actgaaatct ctccttcagt ttctaatgat
    32521 ttattagatg aagaacgagc agataaaaat gaaaacaatc agttatttga agtagaatag
    32581 gaggtcacct gatggaaaat cgacaagaag tacaagaatt aactttagaa gaagtgatgg
    32641 gtgaccgctt cggacgttat tccaaatata ttatccaaga acgtgcactg cctgatatcc
    32701 gtgatggatt gaagccagtg caacgccgga tattatttgc catgaacaaa gatggcaata
    32761 cgtatgacaa aggattccgt aaatcagcaa aatctgtcgg aaatatcatg gggaattatc
    32821 atcctcatgg tgacatcagt atttatgaag cgatggtccg catgagccaa gactggaagt
    32881 tgcgggaagt actgatcgaa atgcacggga ataacgggag tatggacggt gatccacctg
    32941 ctgcgatgcg ttatactgaa gccagattat ccaagttaag tggagagatg ctagcagata
    33001 tcgagaaaga aacagtcgat ctcgtgtgga attttgatga tacagaaaaa gaaccaacag
    33061 tccttccagc tcgataccca aaccttctcg tcaatggatc gacaggtata tctgctggat
    33121 atgcaacaga aatcccgact cataacttag ctgaagtgat cgatggcaca atctatatga
    33181 tcgatcatcc acaagcgagc cttgaaaaat tgatggaata tattcctgga cctgactttc
    33241 caacaggagg gatcttgcaa ggaaaagatg aaatcaaaaa agcttatgaa acaggacgag
    33301 gaaaagttat tcttcgttcg aaaacaaaga tagaggcaat gaagggtaat aaacagcaaa
    33361 tcgtgatcac agaaatccca tatgaagtaa ataaagcgac cttggtgaaa aaaatggatg
    33421 agatccgttt gaataagaaa atcgatggaa tagctgaagt acgagatgaa agtgatagaa
    33481 caggtctgca aattgtcgtc gaattgaaaa aagacgccaa tgctcaagga attttgaatt
    33541 atttgttcaa aaacactgag ctacagatca attacaattt caatatggtc gctatcgacc
    33601 atatgactcc gcatcaagtt ggactgaaag acattttaag aagttatatc gagcatcgaa
    33661 agcaagtcat aacaaaacgt agtcaattcg atttagccaa agctcaaaaa agacagcata
    33721 tcgtagaagg attgatgaaa gctttatcga ttctggatga agtaatcgca acgattcgtg
    33781 aaagtaaaga caaaaaagac gccaaaaaga atttagtcga cgtctttcaa tttacagaag
    33841 aacaagcaga agcaattgtc acattgcaat tataccgttt gacgaacacg gatatcacag
    33901 agcttcaaaa ggaatccgaa agcttgattg cgcaaatcac agagttgaat aaaatccttt
    33961 caaatgacaa agagcttttc tctgttatga aaaaagaatt gcgagaagtg aaaaaaaatt
    34021 attcttccgc tcgtctaacg acaatcgaag acgaaatcga agaaatcaaa attgatacgc
    34081 aagtcttagt ggcacaagaa gacgtaattg tcagtgtgac gcgagaaggc tatgtgaagc
    34141 gaacaagcct tcgttcttat agtgcttcca aaccagaaga aatcgggatg cgagaaggcg
    34201 actacttgct atactcaggt gaattgagta cattggacca tgttcttctg atcacaaata
    34261 aagcaaatgt gatctaccgc cctgtccatg aactacccga tttgaaatgg aaagatgctg
    34321 gtgagcacat ttctcaaaca atctccaatc tgtctgtaga cgaatcgatt ttagctgttt
    34381 tcccatatca aaaaattcaa gcagagaaaa catttgtatt catcagcaag aatggattga
    34441 tcaagcaaac acgaatgaca gagttcgaac catggcgcac ctataagagc cgtccgctca
    34501 gctgtatgaa gctaaaggcg gaagaagatg aactgattgc tgtgtatctg aaagaagatc
    34561 agccaggctt agatgttttc ttagtaacgc atcaaggaat gggtttgcgc tatcctttaa
    34621 cagatgttcc cgttgttgga gcaaaggcag ctggagtgaa gtctatgaat ttgaaagatg
    34681 gagataggat tatcaacggg ctgcttgtgt tagcagaagg tgatacgccg atcgtaatcg
    34741 ttactcagcg aggagctgtt aaacgcatgc ttgctcaaga aatcagccaa acttcacgtg
    34801 cgaagcgggg agtgacggtc atgcgtgagc tgaaaaaaaa tccgcaccgt attatctata
    34861 tgtcggaagg aagatcaaaa gaaataacct tgatgaatca aaaagggcag cagttaacga
    34921 ttgacccaac tgatttccct ataggtgatc gaacatcgaa tggatcattt gcgatggatg
    34981 agaaaaaagg tggagaagtc atcgaagtta tcgatgctcc tgagattttc atcgagtcgt
    35041 aactgttgta gtattttaat gaagagacgc catctgtatt aatacaggtg gcgtctcttt
    35101 ttcattcatt tttaataaat ccttatacgt ttgcaaaaaa agatgaaagt gttcacaatg
    35161 cttaaatgtt ggtatgtcaa cgttgattta aatatatgta aataaaacat aaaaattgtt
    35221 aaataaaaaa caagaaaacc cttgcaaaga tagggtgctg tgttatacta atcacgtaaa
    35281 aactgttaca taaaacagaa atttaggagg tccatacaat ggagcaatgg aatggattta
    35341 aaggtaccaa atggaaagaa ggcgtagaca ctagagactt tattcaaaat aactacactg
    35401 aatatcgcgg tgatgacagc tttttggaag gcccaacaga agcaacagaa aaattatggg
    35461 atgaattgca aaagttaaat gatatccaat tcgagcgtaa cggtgtctat gatatggaca
    35521 cagaaatcgt atcaactatt acttcacacg atgcaggcta tttggataaa gatttggaaa
    35581 aaatcgtcgg tctacagaca gacaaacctt tgaaacaagc attcatgccg ttcggcggaa
    35641 tcaatatggc taacaatgcg ctaaaaagca atggctatac accaaacgat gaattgacac
    35701 atattttcac tgactggcgc aaaacacata atcaaggtgt atttgacgct tatactgctg
    35761 aaatgcgtgc tgctcgtaaa aacaaaatca ttacagggct tccagacgct tacggtcgcg
    35821 gacgtatcat cggtgactat cgtcgtgtgg cattgtatgg aatcgactat ttgatggaac
    35881 aaaagaaaaa agatcatgat atgactggtt atcatacaat gagtgaagaa gtgattcgtt
    35941 tacgtgaaga aatcactgaa caatatcgtg cattagcgca aatgaaagaa atggctgcga
    36001 aatatggcta tgatatttct aaaccagctg caaatgcaaa agaagctgtc caatggctat
    36061 acttcggata tttagcagca atcaaatcac aaaatggtgc tgcgatgtca atcggacgta
    36121 tctcagcatt ccttgatatc tatatccaac gtgatttgga taatggagta atcacagaaa
    36181 aagaagcaca agaattgatc gaccacttag taatgaaatt acgtatggtc aaattcgcgc
    36241 gtacacctga atataaccaa ttattctcag gattccctat ctgggctact ttatccattg
    36301 caggtatggg attagatggt cgttcattag ttacaaagaa tgatttccgt ctgcttcata
    36361 cattaacaaa tatgggacct tcaccagaac caaacttgac tgttttgtat tctgaacacc
    36421 ttccagaagg attcagaaca tatgctagca gaatcgctat cgaaagttct tcgattcaat
    36481 tcgaaaatga taatcagctt cgtgaacaat ggggttctga tgattgtgcg attgcttgct
    36541 gtgtatcagg aacagttgtc ggaaaagaca tgcaattctt tggcgctcgt gctaacttgg
    36601 ctaaaacact attatatgcc atcaatggcg gagtcgatga agtgactggc gcccaagttg
    36661 gaccagaata tcgtccaatc acatcagaaa tactagactt tgaagaagtg aaacataact
    36721 tcatgaatat gatggattgg ttagcagaac tatatgtcaa tacgttgaac atcattcatt
    36781 atatgcatga taaatattct tatgaagcag ctcagctggc tttgatggat tctgacttgc
    36841 aacgcacatt tgctacaggt attgctggga tctctcatgc ggcagacagc ttgtcagcga
    36901 tcaaatatgc aaaagtaaaa gcaatccgta atgaaaaagg aatagcagtt gattttgaag
    36961 tggatggcga cttccctaaa tacggaaatg atgatgaccg tgcagatgaa ttagtttcat
    37021 ggatcttgga atacttcatg ggacaaatca aacgtcataa aacttaccgc aactcaaaac
    37081 caacaacttc attattgacg attacttcaa atgttgttta cggtaaaaat acaggaaata
    37141 ctcctgatgg ccgtgaagct ggaaaaccac ttgcgccagg agcaaatcca agttacaatg
    37201 cggaacaaag tgggttatta gctagcctga actcaacagc taaaatgaat tacggctgtg
    37261 cattggatgg aatttcaaat acccaaacaa tcaatccttc aggtcttgga aaagaacaag
    37321 atacaagaat cgaaaacttg cgtaatgtct tagatggata cttctcaaaa ggagcttatc
    37381 atttgaatgt caatgtattc agtaatgatt tattgcgtga tgcaatggaa aatccaatga
    37441 aatatccgaa cttgacgatc cgggtatctg gttatgcagt taaattccgt gacttgacaa
    37501 gagaacaaca attagatgtc ttgatgagaa cttcacacga cagaatgtaa aaagttcgtg
    37561 aaaaaagttt ttcgacttag cgtcgaaagg acgttgaata gccatcgcat caaaatcgaa
    37621 taatcagtat gcttatttga aagaaacgat ttacaggggc aggacttgta tgttccgtcc
    37681 ctgtaaattt tacacataac gaagcggcaa ttgcgaaaaa ttttgttcag aaagcttcgc
    37741 ttcgacagct aacttgtact taaaatgaaa tcaaagaaaa gatgtgaact taaatggaag
    37801 aaaaaacaat cggctatgtt cattctatcg aaactttcgg ttcagtagac ggacctggac
    37861 tgcgctttgt cgtgtttatg caaggatgcc gcatgcgttg ccaattttgt cacaatccag
    37921 atacttggaa tattggcgga gggaaagaat acacagcaga tgaactttta gataaagcag
    37981 aacgcttcag accttactgg ggagataaag gtggtattac tgttagtggt ggagaacctc
    38041 tattgcagat cgattttttg atcgaactat ttaaaaaagc aaaagagcgg aagatgcata
    38101 ctacattgga tacatgcgga aaacctttta cgtatgaaga acctttcttt tcacgttttc
    38161 aagaattgat gaagtataca gatttactgt tatttgacat caaacacatc gataatgaag
    38221 agcataaaaa actaacgcat tgggacaatg aaaatatttt agaaatggct caatatttgt
    38281 caaaaatcaa caaaccggtt tggattcgcc atgtactagt tcctgagcga agtgattatg
    38341 atgaatattt gatccgacta gacaacttta ttcaaacctt gtcaaatgta gacagagtag
    38401 agattcttcc ttatcatacg atgggaaaat acaagtggga aacacttggt ttgaaatatc
    38461 cattagaagg gatcgaacca cctacaaaag aacgtgtcga aaatgctaaa cgattgcttc
    38521 atacagaaga gtatacaaaa tatttaacgc gataggtcgc atcggaaaga agataagtat
    38581 tgcttctttc cgtttttttg ctattattaa ataggagaaa tcacaaagga gcgaatgaca
    38641 tgtcaaaaat tttagttttt ggtcaccaaa atccagatac agatgcaatc ggggcagcca
    38701 ttgcgttttc acacttgcaa aatgaattag gtaaagaaac agaagcagtt gctctaggta
    38761 cacctaatga agaaacacaa tacgcattag accactttca gctatctgca ccacgagtag
    38821 tagagtcagc taaagcagaa aatgcagatg tcatgcttgt ggatcataat gaattccaac
    38881 aaagtatttc agatatcagc gaagtaaata ttttagctgt agtagaccac catcgtattg
    38941 caaactttga aacagctaat ccattgtact atcgtgcaga accagttgga tgtacaagta
    39001 cgattatttt aaaactatat aaagaaaacc aagtaacaat tcctaaagaa atcgcaggaa
    39061 tcatgctctc agctatcatt tcagatacat tgttgttcaa atcaccaaca tgtacgcctg
    39121 aagatgaagc agctgcaaaa gaacttgcag caatcgctga agtcgatcta aacacttatg
    39181 gacttgaaat gttgaaagca ggaacaaatc taagtgataa atcagctgcg gttcttcttg
    39241 atttggatgc aaaaagtttc ccaatgggca gttccaacgt tcgtatcgct caagtcaata
    39301 cagtggacct taatgaagtg atggatcgac aagcagagct agaagcagca atggaagatg
    39361 aaatgaaagc aaatggctac gatctgttcg tattagttgt cacaaacatc ttagacagtg
    39421 attcagaatt acttgttgct ggtaaaggaa aagaaaaagt agaagaagct ttccaaacaa
    39481 ctttgaccaa caaccgtgct ttgcttaaag gtgtcgtttc acgtaaaaaa caagtcgtac
    39541 ctcaattgac ggctgtattt agctaatcaa atggtagtat gaagcattca aaatgaatgg
    39601 ataagcgatt gatataaaaa gaaacataga acatactaaa ggccgaagaa atggaggaga
    39661 ataacagtca attatgactc ttgttcttcc tcctttttac gtaaaagcct tttaagtaaa
    39721 ataaaacgga tagaaaagag aacatatatg aactattatt caaaaaataa ggaaaacttt
    39781 tatcaaaaac tgaccgatga tccgcttttt tcttcattga ccgattatct atatgaacat
    39841 cgagaacaag aaacgattct aagagaattg aaaaaagaat tttcgcaaaa taaattttcc
    39901 cattttcttg atttattgat cgatgcagga ttaatcaaga gggaagaacg aagatatcat
    39961 ttgaactttc caatatttga tcctaaagat tacttgcaac aagccacttc ggctgctgaa
    40021 accatagctg aacaattgaa gagactctct gttgatgaac aaaaattagc gatgggagaa
    40081 gttatttggg cgtattgttt cgaagatgaa cggaaagaag cgtattttta tggagtaaga
    40141 aacagccgag agacagaatt gctgaggaca actgcaggaa atgagaagta ccgttttatc
    40201 acactttcct ctatagaaca cttcccgctt actttggcta attacttttt tgtacagaag
    40261 aatcagcttc ctgtaacgaa ggcattcaaa gaattagccg aacttattgg agacgtaaat
    40321 gaatcgtact tttttgatca aatagaagtt atcgttgaca ggatacgtaa gaataaatat
    40381 aaaaatcgcc ggcctagtat tttccatcag tctctacttg tgacgaatac tataaaggaa
    40441 gaagaatcat tcacattaga attacctatt gtcgaaaaaa acaattttga gattgaatta
    40501 ccaacattgg atccttcatt gacaatggag gaaatggcct ttcttaaacg acaaatcttt
    40561 tctgagctga gcaagaaatt tataccgcat gcattttctt atatcaaaga atatggaaca
    40621 gttttagtga gtaaaaccta agagagcagt aagcaaaaat tatactgact cttgcaggag
    40681 gagcctttct cttcctttat ggttaaaaga attatttgag tattttgcct atctaccacc
    40741 tattagattc actacatggt ctggaaacaa aggaatgcaa aaaaatggtg agaaatgtgg
    40801 aatgacaaag gaagtagtga taagaaaagt tagattttta aataatcaat attatgattc
    40861 agtcaagtat ggcattttat gggaagaact agctgattag attaggaagt ccatttgata
    40921 atccagaatc actaagaaac gaatggcgag tcattaataa tttatccaat cttgggttat
    40981 tcttttagat tgaagtgttt aaaactaaaa aagcatttat gaagtcgtaa tagtcaggct
    41041 tcaaaatgct ttttattttg tttgtttttt ctttgccaca taaatacacc tagactgacg
    41101 atgaagagca tatccaacag atttcctgcg acttggatcg tcgaagggtt ggtcagtggc
    41161 attaaagcaa ggacaaaaaa ggaaaacaag ctgtaaataa aacaggtgac aggaactaat
    41221 cgaaagaaga gcggacattt caattggcta aaaatgagtg aaaaaataaa ataagtaaaa
    41281 atggataaaa tcacgactaa accattcatg ataaagaaga aaagcgtatt gtgattaaac
    41341 gaataaaaaa gaattgctaa aaaagaaaac gttttaaatg caatcaatac gcttgaagct
    41401 tcgtttaacg taccaggatg tcgatgaaaa aaataggcac cgaaataaac agaaaacagt
    41461 aagccgcaaa gagaagacaa aatcaaaaaa aacggatttt cttcggcttc tgtgatatca
    41521 gaatgatcca aaagcaacca gcctaatgca ctagaaggcg catgtgaggt cttaaggtat
    41581 gaataacata gaaaaaataa gatcgtcaaa gttgcaaaaa aagagcctaa atgtaaaaaa
    41641 ttagtggttc tacgttgaat ttccataata tctccaaatt aaattgataa gtatataaag
    41701 tataataaca aattgatgag tatatgtcga tgttttttaa tcaaaataat aaataaaaac
    41761 tgtctaacat ttaaaattta
//
LOCUS       DAFCLG010000010        41765 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFCLG010000010 DAFCLG010000000
VERSION     DAFCLG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.010"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(357..2738)
                     /locus_tag="LND56_000554"
     CDS             complement(357..2738)
                     /locus_tag="LND56_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6742502.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2728..3375)
                     /gene="recU"
                     /locus_tag="LND56_000555"
     CDS             complement(2728..3375)
                     /gene="recU"
                     /locus_tag="LND56_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBK6742503.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3439..3978
                     /locus_tag="LND56_000556"
     CDS             3439..3978
                     /locus_tag="LND56_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBK6742504.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4068..4487
                     /gene="gpsB"
                     /locus_tag="LND56_000557"
     CDS             4068..4487
                     /gene="gpsB"
                     /locus_tag="LND56_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBK6742505.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4509..4876
                     /gene="rnpB"
                     /locus_tag="LND56_000558"
     ncRNA           4509..4876
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LND56_000558"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5085..6251
                     /locus_tag="LND56_000559"
     CDS             5085..6251
                     /locus_tag="LND56_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBK6742506.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6297..7793
                     /locus_tag="LND56_000560"
     CDS             6297..7793
                     /locus_tag="LND56_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBK6742507.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7874..8557
                     /locus_tag="LND56_000561"
     CDS             7874..8557
                     /locus_tag="LND56_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6742508.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9202..10392
                     /locus_tag="LND56_000562"
     CDS             9202..10392
                     /locus_tag="LND56_000562"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK6742509.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10494..11447
                     /locus_tag="LND56_000563"
     CDS             10494..11447
                     /locus_tag="LND56_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK6742510.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11516..12865
                     /locus_tag="LND56_000564"
     CDS             11516..12865
                     /locus_tag="LND56_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6742511.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13048..13371)
                     /locus_tag="LND56_000565"
     CDS             complement(13048..13371)
                     /locus_tag="LND56_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742512.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13500..14663
                     /locus_tag="LND56_000566"
     CDS             13500..14663
                     /locus_tag="LND56_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6742513.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14733..16082
                     /locus_tag="LND56_000567"
     CDS             14733..16082
                     /locus_tag="LND56_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6742514.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16127..16558)
                     /locus_tag="LND56_000568"
     CDS             complement(16127..16558)
                     /locus_tag="LND56_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742515.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16946..19588
                     /gene="alaS"
                     /locus_tag="LND56_000569"
     CDS             16946..19588
                     /gene="alaS"
                     /locus_tag="LND56_000569"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK6742516.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19788..20384
                     /locus_tag="LND56_000570"
     CDS             19788..20384
                     /locus_tag="LND56_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6742517.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20506..21654
                     /locus_tag="LND56_000571"
     CDS             20506..21654
                     /locus_tag="LND56_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6742518.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21692..22105)
                     /locus_tag="LND56_000572"
     CDS             complement(21692..22105)
                     /locus_tag="LND56_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBK6742519.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22184..22615
                     /locus_tag="LND56_000573"
     CDS             22184..22615
                     /locus_tag="LND56_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBK6742520.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22714..22947)
                     /locus_tag="LND56_000574"
     CDS             complement(22714..22947)
                     /locus_tag="LND56_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBK6742521.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23130..24800
                     /locus_tag="LND56_000575"
     CDS             23130..24800
                     /locus_tag="LND56_000575"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBK6742522.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24947..25333
                     /locus_tag="LND56_000576"
     CDS             24947..25333
                     /locus_tag="LND56_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742523.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25320..26018
                     /locus_tag="LND56_000577"
     CDS             25320..26018
                     /locus_tag="LND56_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6742524.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26023..26487
                     /locus_tag="LND56_000578"
     CDS             26023..26487
                     /locus_tag="LND56_000578"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK6742525.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26484..27395
                     /locus_tag="LND56_000579"
     CDS             26484..27395
                     /locus_tag="LND56_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6742526.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27630..28169
                     /gene="pyrR"
                     /locus_tag="LND56_000580"
     CDS             27630..28169
                     /gene="pyrR"
                     /locus_tag="LND56_000580"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBK6742527.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28183..29466
                     /locus_tag="LND56_000581"
     CDS             28183..29466
                     /locus_tag="LND56_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK6742528.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29479..30405
                     /locus_tag="LND56_000582"
     CDS             29479..30405
                     /locus_tag="LND56_000582"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBK6742529.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30424..31704
                     /locus_tag="LND56_000583"
     CDS             30424..31704
                     /locus_tag="LND56_000583"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK6742530.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31709..32785
                     /gene="carA"
                     /locus_tag="LND56_000584"
     CDS             31709..32785
                     /gene="carA"
                     /locus_tag="LND56_000584"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK6742531.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32790..35975
                     /gene="carB"
                     /locus_tag="LND56_000585"
     CDS             32790..35975
                     /gene="carB"
                     /locus_tag="LND56_000585"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK6742532.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36029..36805
                     /locus_tag="LND56_000586"
     CDS             36029..36805
                     /locus_tag="LND56_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBK6742533.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36802..37734
                     /locus_tag="LND56_000587"
     CDS             36802..37734
                     /locus_tag="LND56_000587"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBK6742534.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37731..38447
                     /gene="pyrF"
                     /locus_tag="LND56_000588"
     CDS             37731..38447
                     /gene="pyrF"
                     /locus_tag="LND56_000588"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBK6742535.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38454..39083
                     /locus_tag="LND56_000589"
     CDS             38454..39083
                     /locus_tag="LND56_000589"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBK6742536.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39071..39385
                     /locus_tag="LND56_000590"
     CDS             39071..39385
                     /locus_tag="LND56_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742537.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39423..40064
                     /locus_tag="LND56_000591"
     CDS             39423..40064
                     /locus_tag="LND56_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6742538.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40107..40799
                     /locus_tag="LND56_000592"
     CDS             40107..40799
                     /locus_tag="LND56_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBK6742539.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40796..41698
                     /locus_tag="LND56_000593"
     CDS             40796..41698
                     /locus_tag="LND56_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6742540.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        13496 a         7351 c         9102 g        11816 t
ORIGIN      
        1 taagcatact atcagataac tcaaaatgaa gccttctttc aaaaaattat tcaaaaccat
       61 atattgtatt ttttatttga taataacaat gggattatta agaagtggca ctaaataaaa
      121 gactagtgtg tatttagccg atttaattat aaacaagtaa atggataaag tatatgtaat
      181 gataaaagaa aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg
      241 ctggatagtc ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct
      301 gttttttaca tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat
      361 ctgtttccag aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg
      421 ctagttgctg gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct
      481 tcggatgaag aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg
      541 ctttcttttg aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc
      601 gttgtctctg ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt
      661 gatggtgtaa ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc
      721 acacgaatta atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac
      781 tgcataagct cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggtgtc
      841 atttttttat tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat
      901 aggatatctg gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa
      961 ttggatgttc cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt
     1021 ccttcagtga tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg
     1081 gctgtttttc catctggctc ataagtttct tctgttccgt cttggaagac gattttatta
     1141 acatattgag gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt
     1201 tttaaggaag aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca
     1261 ttcgattgat ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta
     1321 tctgaaccga cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct
     1381 tggcgtaaag taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc
     1441 ccttcatagt tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca
     1501 aaagctggtc cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc
     1561 actgctaagt tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct
     1621 tttactttcc cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg
     1681 taagagacat actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc
     1741 aaattcgtat aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact
     1801 tcattgatga cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt
     1861 agttcttgca gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag
     1921 attttttcgt tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct
     1981 ggataaacat aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt
     2041 tgcggtaaag aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc
     2101 ccgtacaagc cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc
     2161 gatttttttt gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc
     2221 tgatcctcgg cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt
     2281 ccgccttgca gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg
     2341 tcaaccccga tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat
     2401 gttttcggta attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat
     2461 agttcgccat cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc
     2521 aattcgggcg catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata
     2581 cagaaaaatg agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca
     2641 gaggactttc cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt
     2701 ttctgtcgtc tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga
     2761 taatgttgat aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt
     2821 tttgatctct attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc
     2881 cttttgggcg tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa
     2941 agacgaaaac cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg
     3001 ttcgatttga tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt
     3061 tgcttcaaag tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg
     3121 ggtaaagtag gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat
     3181 ttggataggg gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt
     3241 actttcattg attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt
     3301 tcttttttct tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg
     3361 atagcgcaat accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag
     3421 aaagaaaagg aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt
     3481 ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta
     3541 aaaaaagaac tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat
     3601 ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa
     3661 ctatctcttg gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac
     3721 caagaaaaaa aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa
     3781 ccctaccaat cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg
     3841 ggctgtctgc tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat
     3901 gcacagaatt accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt
     3961 caaaatttta gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg
     4021 aaaaaggtag aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg
     4081 tttatagtcc taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc
     4141 ccgttgaagt tgatgaattc ttagacaata tcatcaaaga ttatgaaact tacagcaaag
     4201 aactccttgc tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta
     4261 aaactcaagg agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag
     4321 tgaccaattt tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa
     4381 agcttgatca acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa
     4441 atgcagacac aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg
     4501 aatttgcggt tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc
     4561 gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt
     4621 tggagtaacg gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg
     4681 ccacagtgac gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg
     4741 agcaacccaa acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca
     4801 gtataactgc agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag
     4861 aacatggctt atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt
     4921 tataagaaac aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt
     4981 attacaaaac aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag
     5041 gactgatgtc ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga
     5101 agttcaacct cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa
     5161 gagatcttgg aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg
     5221 aaacgatcgc tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg
     5281 gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg
     5341 aagattttct tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac
     5401 tatatagcac acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta
     5461 cctattatca ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg
     5521 aagtagcttt gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat
     5581 tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag
     5641 cacttattat gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt
     5701 caggtacgct ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc
     5761 gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat
     5821 cagcagccga agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca
     5881 atggacggat gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga
     5941 ttacctttaa gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg
     6001 tggctaatcc gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata
     6061 aagaaatggg cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg
     6121 acttagcgtt tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg
     6181 gtgcattgcg tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga
     6241 aagacgaata atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga
     6301 aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa
     6361 tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag
     6421 tagacagcta tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag
     6481 ccattcgcta tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg
     6541 ccaaagtaaa agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat
     6601 atacagctgc cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat
     6661 tttctgattt tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt
     6721 actggagaaa agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga
     6781 tgactgtcga aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc
     6841 gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga
     6901 cgaaaaaaca acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta
     6961 gtcgtggtcg tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg
     7021 cacgaatcac gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc
     7081 acgaggctgg tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg
     7141 tttatgaagg tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta
     7201 ttggaagtag tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag
     7261 gaacattcga tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa
     7321 gtttggttcg tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg
     7381 aaatcgaaaa aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga
     7441 atcaaaaata tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac
     7501 aagacgttca ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca
     7561 tgtatgcggc acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag
     7621 cgtcagacga ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg
     7681 catctagaaa accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat
     7741 acctgatcga ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa
     7801 tttacaaaga tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa
     7861 agaagtggaa cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc
     7921 gcatgatcaa acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa
     7981 aattttggag acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca
     8041 tgatacgatt gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt
     8101 attccttcgc tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga
     8161 aaatatgtcc ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat
     8221 cgttcaagac gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta
     8281 tttcggcggt ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa
     8341 ggacaaagaa aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt
     8401 cttgctgtct gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac
     8461 cttcatgcgt gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt
     8521 tactccagac aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc
     8581 agctccaaga tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa
     8641 gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc
     8701 tcgtgtttga tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttattc
     8761 ggaagaatag gactgtgaca agacttttgt cacagtcctt ttttcgaatt caacaagtaa
     8821 aacttttaag aaatattcag agagtaaaat taaaaattgc tgataaacga agatggaaat
     8881 tttatgtatt tttattggtc tatgcaataa ataaattaaa tattcagaat acaattgatt
     8941 ttaacgaaag ggcttctatc aatggttgtg ttgtatttat ctgcccaggt agagtaaaat
     9001 agatcattat ctaaaaggta attgttcata ctatataagt catttgaaag taaaaagata
     9061 ctgtaaataa aaaatgtggt taaataattc tcataatcag tgtaagcgtt atcaaaaata
     9121 tagcatactt gctttgtaga tgaatgacac gatatatgtt tggtatgttc tgcaagacag
     9181 atgaatatgg aggtggagac aatgtcttcc aaagaactaa caatgtattt agagaaagaa
     9241 ttggatcaac tgaaagaaaa ggggttatac aatacgatcg acatactaga aagtgaaaat
     9301 ggtgcttgta tcatcgtaga cggaaaaaag atgatcaatc tagcatccaa caattatctt
     9361 ggatttgcca atcgcgaaga attgaaaaaa gcatgtattg aagcaaccga aacttatggt
     9421 gtaggagcag gagcagtccg aacgatcaat ggttcgctga aaatccatca gcaattagaa
     9481 gaaaaaatcg cagaattcaa agggacagaa gcagcaatcg cctttcaatc tggattcaac
     9541 tgcaatatgg gcgctatctc tgcagtaatg acaaaagaag atgctatctt atcggatgaa
     9601 ttaaaccatg catctattat tgacggttgc cgcttatccg gagcaaaaat catccgaatt
     9661 aaacatcaag atatgaaaga ccttgagaaa aaagcaaagg aagcgataga aagtaagaaa
     9721 tataaaaaaa tcatgtatat cacagatggt gttttctcca tggatggaga cattgcacga
     9781 ttgccggaaa ttattccgat tgttgaaaaa tatggactga tcacctacgt cgatgatgcg
     9841 cacggctcag gcgtaactgg aaaaggtgca ggaaccgtca agcattttgg attaagcgac
     9901 aaaatcgata tgcaaatggg cacgttatcg aaagctatag gtgttgttgg gggttatgta
     9961 gctggatcga aaacactaat cgactggttg aaagctagaa gtcggccatt cctattttct
    10021 acttcgctaa ctccaggtgc agctgcagct gcattggctt cgattacttt gatgcaagaa
    10081 catccggaac ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga agaattgaaa
    10141 aaagtaggct ataacatcgg tatgtctgaa acacctatta ctccagtcat tttgggagac
    10201 gaaaaagtga ctcagacttt ctctaagaag ctgatcgaac atgggattta tgctaaacca
    10261 atcgtttatc caactgtgcc tcttgggaca ggcaggatca gaaatatgcc gactgcggaa
    10321 catacgaggg aaatgcttga tgaagcagta gcagtttatc aaaaaatagg aaaagaaatg
    10381 gaaattattt aactaaattt agttttttcg tctatttaaa attagagaac atgttcacgc
    10441 atatattgtt aatcatctga agatagtagt aaaaaagatc agggaggatc atcatgaaaa
    10501 aagtattggt gacaggatgt ttaggacaaa ttggttcaga gttgatcaca cggctacgtc
    10561 aagaactagg cagtgaaaat gtcattgcga cagatatacg catgcctgaa gtcattgaga
    10621 aaaacgatat ctttgagatc ctagatgtaa tggattacga aaaaatgcgc agcatagcag
    10681 aacaatatca agtggacaca ttgattcatt tggctgctct tttgtcagct gttgcagaag
    10741 caaaaccaca attagcctgg aatttgaacg tgactgggct ggtcaatgca ttggaagtcg
    10801 ctagagaatt ccagctgaaa ttttttacgc caagttcaat cggcgcgttt ggtccaaata
    10861 ctccaaaaga tcatacccca caagatactc ttcagcgtcc tacaaccatg tatggggtaa
    10921 cgaaggtagc tggagaacta ctatgtgatt attattatgt gaaatatggc gtagatactc
    10981 gtggtgtccg tttccctgga ttgatttctt atgcaacctt gccaggaggc ggtacgacag
    11041 actacgcagt cgatatttat tattcagcaa tcaaagaagg aaagtacact agtttcattg
    11101 atcaaggtac atcaatggat atgatgtata tgccagatgc gatcgatgcc attataaaac
    11161 tcatgaatgc agatcctgat cgattggttc atcgaaatgc cttcaatata tcggcgatgt
    11221 cttttgaccc ggaagagatc aaagcatcta ttcaaaaaat cataccagat tttgaaatgg
    11281 attatgatgt ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat tcactagatg
    11341 tttcttgtgc gcaagaggaa tggggctttt cgccgaaata cgacttagat gtgatgacaa
    11401 aggatatgtt ggaaaaactg aaagaaaaat tggtagagac ggtataaaac aagaaccaca
    11461 tccaaaaagt tttttatact tttgggattg gctatggtat gaaaggggac aagggatgga
    11521 agaatatcaa gccacgccag taaaagaagt aaaaatagaa aatgaattaa aacgtacgat
    11581 gggatttttt actgctttat cgactgttat gggcacagtg atcggtgcag gggtattttt
    11641 taaagccgca agcgtagcag aagttacagg atccgccagt ttgcatatgt tttcttggtt
    11701 tctcggtggt atgatttctg tctgtgcagg actaacagga gctgaactgg cagctgctat
    11761 tccagaaaca ggagggatga ttaaatatat cgaacggatt tacggaaata cagcagcttt
    11821 tttattgggc tgggcgcaag tcgtgattta ttttccagca aatgtagcag ccttgtcgat
    11881 cattttcggt acacagttcg tcaatctatt tggtctttcc cagtcaatga ttgttcccgt
    11941 agctgttaca gctgctgtat cgatcctgtt gatcaatttt ttaggttcta aagctggagg
    12001 ggcttttcag tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga tcgtcatttt
    12061 cggtttgttc cgacaagaag gagtagactt ccaactgttt ccgatccaag caggagaaaa
    12121 tctttcattt ttctcagcct tgggagcggg cttgctagca acgatgttcg catacgacgg
    12181 ttggatacac gtaggaaata tttcgggaga attgaaaaag ccggcgaaag atttgccaaa
    12241 agcaatttct ttaggaatca tcggaatcat gatcgtttat ttattagtaa atgcggtgtt
    12301 cttaagaacc gcatctatcg acggagtcgc aggaaacagc aatgcggcaa gtgacgttgc
    12361 aaaaatgatt tttggcggct tcggtggaag actggtcaca gttggtatct tgatttctgt
    12421 ttatggaacc atcaatggct atacccttac tggaatgcgt ctcccttatg ttattgcaaa
    12481 agaaaacaac ctgcctttta gcaagctctt tgctaaactt catgataaaa ctaaagttcc
    12541 tgtagccgca ggtatcttag agctagtcat cgctattggc atgatgatga tcggtggttt
    12601 tgatacacta acggatatgc tgatctttgt catttggatt ttttatacga tggtctttgt
    12661 tggtgtcatt cttcttcgta aaaaagaacc tgatttgttt cgaccataca aagttccaat
    12721 gtatccgttt attccactag tagcaatcat tggagggact tttatcgtat caagcacgct
    12781 tatcactcag acctttttag cttcgatggg gatagtattg acacttgctg gtatcccgat
    12841 ttatctctat ttaaaacgta gatagctaaa acattcgaat tttattagaa ctgcacttct
    12901 ctgccaaaca aaatatttta tttaaatgag gagtacgtgg aagaaatgtc aaaagtactc
    12961 gttaattttg atcacgtaaa tgtcgttttg tctacgatga aatagcaaag taaaagacag
    13021 aaatcgggct caaccgattt ctgtctttta ctttgatttg cgtaactgaa ctattcggcg
    13081 tacgccgcga taaatattta ataaaaccaa gcaaccagaa atgaaaatgg gaagccaaat
    13141 cagatagaca gggtagccaa gcactttaga caaagcatgt ggagtcattt tatccatttc
    13201 aagaagtgct tgctgccaag tctgaagatt gaagactagc atgaaacccg aaataatcag
    13261 gagaataaat cctaaataat gaattgtagt tctttttctt acataagcga tcgtagcata
    13321 aaggaacagt aaaaaaatag gtacgccaaa aacaaagtac atattcagca tctcgacacc
    13381 ccctttgtgt ttatcttact ttactttgaa agaaatgaga aataatagcc tcgttacttg
    13441 cttgaaacag caaaggactg ctaagataag agagatacaa agaatcaagg aggagtcata
    13501 tggcaagtta tatcgaaccg attcgttttg cgctgattgt ttttccattt ttagccttag
    13561 cgatctcatc tgtttttttt atttatgaat atcgaaagta tggaacattt ctagtaacaa
    13621 gggcagttat tttgtattct ttcgtttttt atttattgtg tgcgtatttt ttggtgatct
    13681 taccacttcc ggcaaaggag acggttgccc aaatgacagg gccaaagatg gagctgcatt
    13741 tatttgcttc atggcaacat ttcagagaca acacggtatt ggtcttgtct gaacctagta
    13801 cctacctgcc ggcaatgaag cagagtgtat ttttggaacc agcttttaat gttttattac
    13861 tcataccatt tggcatttat ttaagatatt atttccggct ttcttttatg aaaacgatga
    13921 ttgctagttt ctgcctttct ctattttttg aattgacgca gctaagtgga ctttatttta
    13981 tctacccaag accataccgc ttatttgatg tgaatgattt agtgacaaat acattgggtg
    14041 gaatcattgg ttttgtcgtt acgccgttat ttacatttat gcttcctaca agaagacgaa
    14101 tggatgaact ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc ttggtcgctt
    14161 atttgatcga ctggacattt ttatctctta ttaatttggt agcggcgatt ttctttaaaa
    14221 ttgcgttcga ttcaggggca gttacttctc atatctggtg gcttttatta gaagtagggg
    14281 tatactttat gattgtaccg tttataacca atggatggac acttggcaaa aaaattgtgc
    14341 gtatccgttt ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg atgatccgct
    14401 acgggtatct gtactttctt ttttatggtt taagctggtt ggggcagttg cttcagcaaa
    14461 tcatgaattc gacaacgaat acggtgttgc agatgagtgc agtattcatt tctctcggtg
    14521 tcttgttact acaaggtatt tttattttaa atatcttgct atcatttttg agaaaaaaac
    14581 ggattctgtt ttatgaaaaa gctagtcaca catatactgt tagcacgatt ggccaaacaa
    14641 aggatgatga ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta taatgccaaa
    14701 gtcgtgataa tttaaagaga agaggtgcac gaatgaataa ttttgatata ttccgcttca
    14761 aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa acagcccaca gaggtccagg
    14821 aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct caaactggta
    14881 caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca gaaaaagagg
    14941 aagtacaagt tgttattaca gctccaagta gggaactagc tgcacagatc tatgaagcag
    15001 ctacacaaat cgctaaattt tctacaccgg agatccgtgt ctcgaatttt gtcggcggta
    15061 cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt gtagtaggca
    15121 cgcctggtag aatattagac atgatgaatg aacaagcgct gaagattcac accgcatttg
    15181 catttgttgt cgatgaagcg gatatgacat tggatatggg ttttttaaat gaagtagacc
    15241 aaattgcaag tcgattacca gaaaagctgc agtttttagt tttttctgcc actattccag
    15301 aaaaacttcg tccattctta cgaaaataca tggaaaatcc aatcattgaa gaaatcaaac
    15361 caaaagcagt tatttctgaa gacattgaaa attggctgat ttcaacaaaa ggtaaagatg
    15421 agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc ttatttagca atcgtatttg
    15481 ccaatacaaa acagcatgtg gatgaaattg ccgattactt gaaaaaccaa ggcttgaaag
    15541 tagcaaagat tcatggagac atcaccccaa gagagagaaa acgagtgatg cgacaagtac
    15601 agaatctgga gtatcaatat gttgtagcga ctgatttagc tgctagaggg atcgatatcg
    15661 aaggtgtctc tcatgttatt aatgcagaaa ttccagaaga tctagacttc tttatccatc
    15721 gtgtcggacg tactggacgt aatggcttaa aaggaacggc cattacgtta tattcgccaa
    15781 atgatgagca agccattgat gcgatagaaa aattgggcgt ctcatttgaa ccaaaagaaa
    15841 tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa cgagaaaaat
    15901 caaaagatgt attagatccc acgctgattg gtcttgtgaa gaagaaaaag aaaaagatca
    15961 agcctggtta taagaaaaaa atcgattggg caattgcaga aaaaaataaa aaagaacgca
    16021 aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat tcacataaat
    16081 aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa gctagacttt
    16141 ttttcattcc gatacaaagt acttcatctt caataaaagg ttctgtgcaa gtatgatacc
    16201 cttgtttttc ataaaaatct acagccgtca tttcagctac tacataacta gtctgacaac
    16261 cttctgaaac cgcccgtttt tcagcagctt caagcaactg accgcctaac ccttttcctc
    16321 ggtattcttt tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt ttcttttcat
    16381 aacgcaaagt agctactggt cttttttgat caagaaatag ataataggtt tgttgcggga
    16441 tatcttttcc gtcgaattct agttctgcag gaatcccttg ttctaataca aaaacttgat
    16501 atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt tttttcatca
    16561 tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta tcgtaccaaa
    16621 aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga cagtgatcaa
    16681 tagaatgcgt atactaatta agtacgaaaa ggatatgatt tgttttttaa gactcacaga
    16741 aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct ttttctattt
    16801 tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga ttacacatca
    16861 ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt tttttgtatt
    16921 taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag ttcgacaaat
    16981 gtttcttgat ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt ctttgatccc
    17041 cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga aaaaatattt
    17101 tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat ctattcgaac
    17161 aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg agatgctggg
    17221 taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat gggaattttt
    17281 gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag tttatccaaa
    17341 agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag atcacattat
    17401 tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg atacggaaat
    17461 tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg aaaactatcc
    17521 aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag aattcaacca
    17581 taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg ggatgggatt
    17641 ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag acttgtttat
    17701 gcctattatc cacgccgtag aaaagctaag caaacaagta aaatatggtg aaaacccagt
    17761 aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat ttgctattgg
    17821 cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt tgcttcgtcg
    17881 tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc gtttagtccc
    17941 tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa aagaatttat
    18001 tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg aaggtttgga
    18061 aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc tagcaggaaa
    18121 agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg aagaggtggc
    18181 agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg atgcacaacg
    18241 cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat ctgctttatt
    18301 gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat cagatagtca
    18361 attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag gtgaagctca
    18421 aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag cggatcaagg
    18481 atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc aaaaagcgcc
    18541 taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag gacaaaccta
    18601 tcatctattg atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata cagcaaccca
    18661 tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag ctggttcttt
    18721 agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca cttcagatga
    18781 attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc cagttgaaac
    18841 aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt ttggtgaaaa
    18901 atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac tgtgcggagg
    18961 cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg aatcaggaat
    19021 tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg aactacttga
    19081 agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc aattgaaaga
    19141 agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa aggaaaatga
    19201 acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag atccagaaac
    19261 gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga atcagctgcg
    19321 tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt tagcaaacga
    19381 acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg aagctggact
    19441 aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg gtggtggacg
    19501 tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg cacttaaagc
    19561 tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa acaaaagttc
    19621 aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc ctcatacttt
    19681 tttcaattgg atggcgcatc taatcaaatg gtcggattcg gatggtcttt agttgacgaa
    19741 agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg aatagtagaa
    19801 ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc gtacaagcga
    19861 tggttccttg gctggggttt attccattag gattcattag tttaacaatt atccatatca
    19921 cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt ggcttgtttt
    19981 ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt gatacattag
    20041 tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta gtagcaggct
    20101 tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca gcctttgcag
    20161 gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga ttattttata
    20221 caggtgcaac ggcagatgcc tatggtgtag actctgctct tttatttaaa accttagcag
    20281 gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata acaccattac
    20341 tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca tcttttaata
    20401 gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa tcgtcctctc
    20461 catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc taagaaacaa
    20521 gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact agcgggtatc
    20581 ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg gcttttttca
    20641 ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc ttcatccata
    20701 ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca tccttatgga
    20761 cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac ttatgtagga
    20821 tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt ctctttaaca
    20881 cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca aggcaaaatg
    20941 tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc aacagatagt
    21001 tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga atgggtaact
    21061 ggatggcgta ttgatggata tataggtttt gctttagcat tatatatcct ttatagtgga
    21121 ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac gccagcagag
    21181 attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta tcatgattta
    21241 ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga agtagatgaa
    21301 tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga ctttaaaaag
    21361 tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa cgaaaaatac
    21421 tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg gcttaatatt
    21481 catgatttta gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt ggtccctgag
    21541 aaattccagt ttgttgatac agaactagaa agaatcttac gtagtcaaat agaagaaaaa
    21601 atcggagatt accaattgga aattacgttt gatcacaatt atcttttata ttaaatgaga
    21661 aactataaaa agactcctta aagtttttaa tttaaagagt ctttttttct tttaattgtt
    21721 tatggagagc ctgacgtgca agattatctg ctcctttatt tttgttttcg ggcaaccatt
    21781 gcaggatcaa taaagaaaat tcggatagaa gttcttggat tctttgaagg tagggaagga
    21841 agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg ctgtctgaaa
    21901 aacagaaaat cgtttcattc tgtaagtcgt ttttctttag aaattctaaa gtttttaaaa
    21961 agacttcgaa ctctgcttga tggtttgtcc ctatagaaag cgggatagat agctgttctt
    22021 gtttaccttg atagacaatc aagattccac ctccactagg accagggttg ccttttgtag
    22081 aggcatcaat gtatactttt aacataaaaa gagattctcc tttttgttca aactaatgtt
    22141 atggtaagat tatatcatta ctgaatcggt gagaggttag agaatgaaaa aaaatattcg
    22201 atggcagcct gaactggcac atacgatcat ctattggtca ttgaccttga tcattctgtt
    22261 ttttagtctg attctttctt tagaaaacac aggtccttat tggaaaagca attttgtttt
    22321 aggtattttt cttgtttttc tatttctagg tttgcggcgt tcttttattt taaaatcaaa
    22381 ctctgtgatt atacgttatg ctgctttttg gaaagaccga gaaatatctg tcggacagat
    22441 tcgatctttg acgccaacta atactggggt gcaaatgatg ttgcaagatg agaaagcccc
    22501 atctgttttc atgatgcgaa aaaaagatca agtagtcctt tttgatcatt ttgaaaaaaa
    22561 cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa gctaatttta
    22621 aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga aaagagtgag acgtacggtt
    22681 catcccgttt agcgtctcac tctatttgtt tatctattca tgcatactag tatcggtagt
    22741 ctgaacgttg cctttcttga catgggcagc ttgtgttcct cgattccctt cgatgatctc
    22801 aaattcgacc acttgatttt cttccaaagt tttgaatccc tcttcttcaa tcgcagtaaa
    22861 atgtacaaaa atttcttctg tgtcatcata actgataaag ccgtatcctt ttttgttgtc
    22921 aaaccatttt acaattcctg tggtcaaagc actcacgacc tttctaaagt ctgatcatag
    22981 ccttcattat aaagagtatt tataagtacg tcaaataaat taaaaacctg taattttatg
    23041 ttttttttct gaataaaaaa aggtataatt ggcatatctt aacaaagaag atattttgtt
    23101 gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt tcacaagaaa
    23161 cagagctaga accgattgtt gaagtggcta gtaaattaga tcttaaggaa gacgatttgg
    23221 aactgtatgg gaaatataag gcgaaaatca atttttctgg aatcaatcga tcaaaggata
    23281 acgctgaggg gcatttgatc ttagttactt ccatcaatcc aacaccagct ggcgaaggaa
    23341 agtcaacgat cactgtaggg ttaggagacg cgctcaataa aatcaataaa aaatctgtta
    23401 ttgcattgcg agaaccctca cttggtcctg tgatgggaat caaaggtggt gctgcaggcg
    23461 gaggatatgc acaggtattg cctatggaag atatcaactt gcattttact ggtgacatgc
    23521 atgctatcac aacagctaat aatgcattat ctgctctttt agataatcat attcatcaag
    23581 gaaatgaatt gaacatcgat gcccgccgag tgatttggaa acgggtagtc gatctcaatg
    23641 atcgtgaact gcgtaaagta attgttggct tgggtgggcc aatccaaggt gttccaagag
    23701 aagatgggtt tgatatcaca gttgccagcg aaatcatggc tatcttgtgt cttgcggcag
    23761 accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt cggctataca tttgaccgtc
    23821 agcctgtgac tgctggagac ttgaaagcag aaggcgctct ggcattactg ctgaaagatg
    23881 caatcaagcc gaatcttgta caaacaattt acggaacacc agcttttgtc cacgggggac
    23941 cgtttgccaa tatcgctcat ggctgcaata gtattttagc taccaagaca gcattgagat
    24001 tagcagatta cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga gagaaatttt
    24061 tagatatcaa agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt gtcgccactg
    24121 tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga acttaaaaat gagaatcttg
    24181 atgctttgaa agtaggtttt gcaaatttaa agcgccatat acgtaatatg gaacaatatc
    24241 agctacctgt gatcgtcgca atcaatgaat tcgtaacaga tacagacaat gaactcacac
    24301 tgttggagca cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt gtatgggcag
    24361 atggagcaga aggcggcgtt gatttagcaa aagctgtagt acgattgatt gatagaaaag
    24421 aagctaatta caagccatta taccgcctag aagaaacgat acaagaaaag acagaaacaa
    24481 tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag caaaaaagcg caaaaacaaa
    24541 tagaagagtt tacgaagaat ggatgggaca aactgccgat ttgtatggca aaaacacagt
    24601 attcattttc tgatgatcct tcactgctag gtgcaccgga agatttcacc atcacgatcc
    24661 gcgaaatcat cccaaaatta ggggcaggat ttttagtagc cttgactgga gatgttatga
    24721 cgatgcctgg tttgcctaaa aaacctgctg cattgaatat ggacgtgaca aatgacggag
    24781 aagtgttggg attgttttaa gcttgattat aatctaccat cgaaggagct gtattttgac
    24841 tacagcttct ttttttagac gatagggaat taattttgtt tacactagga tcattctttt
    24901 tctttattgt attttgagct atactaaagc ataataggag ggtatgatga aaacgacatt
    24961 tataagaaaa accggttttt acggtatggg tagctcgatt cagctaagaa tcaacaatga
    25021 aaaacgcagt gttctcagtc atgaacaaaa gataacgctt gatctggaag tgccctttac
    25081 gatgcaggtc accttttatt ggctaaaaag ccccgtttat acagtgaaag aagcaaagcc
    25141 tgcttatgtg atcacaatga attacttgat tcttcaaatc tatccgtttt tatttctagc
    25201 aactgggttg ggggccgttt tcctacaaaa tttatttttc agtttttttg cagttattgt
    25261 gttgattgcg ttctttttct atataaaaaa ccgggcatat ttgatcaagg aggaaaatta
    25321 tggcaaactc tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg atgagagacg
    25381 agatgggaaa tgcacgaaat atgggattta ccgaacttgt cagacgccta gctcaaagaa
    25441 aacaattaat gatcagaaaa tacgaagatg atcttttaca gcttgctcag ttaaggaatg
    25501 cgatcgtcca tgatcggatc gcagtcgatt ttgtcattgc agagccaaat gattggacag
    25561 tcaagcgaat ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg ctgcctcgat
    25621 ttgcaaaacg tgtaacaggt tttgaacaag atcttccgtt acttgaactg ttaaagatag
    25681 tagcagaaaa aaggtattca caatttccgt tatacaataa aggaaaattt gtagcattga
    25741 ttactttacg taatatcggg ttttggctag caaaagaaag ccaaaaaggt ccggttgatt
    25801 taacaaataa acgagcgttg gatttgatca tccaaaacgg aaagtacaca aattatcatt
    25861 ttgttccagc taaaacacat atttacgagg ttgaagcgat gtttcgtgag caagggactt
    25921 tggaagccgt acttatcact aaagatggga atcccgatgg taatctgctc gggatcgtac
    25981 gacctcgtga tatttataaa caaatagaaa aggattgatt ttttgttaat tatttattgg
    26041 atattcagcg ctttgctagt aggagtggat cagttggtca aatggtggat cactgataat
    26101 ctagcgcttg gagagacgaa aaatcttata ccaggtattt tgtcactgaa tcatattcga
    26161 aacactggag ccgcatggag tatgctagaa gggaagatgt ggttctttac tatagtgaca
    26221 ctcattgcag tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa aggtaatcgc
    26281 tggttcatga ttggattgag tttgattcta gctggtgcga ttggcaattt tatcgaccgt
    26341 attcgtcttg gctatgtagt agatatgttc cagacagatt ttatgagctt tccgattttt
    26401 aacgtagcag atgtgacact tgtatgtgga gtgatttgta tgttaattta tattatttta
    26461 gatgaaaagg atcaaagaaa aaaatgacag aacttaaaac gacgattcac catgaatctg
    26521 gtaggatcga taaagtattg actaatttat tcaccgatta cagtcgttcg caaattcagc
    26581 tgtggctgaa aaatggagcg gtttctgtca atggacagcc agtcaaagca aattacaaag
    26641 taaaacaagg agacgagatt actgtcttga tccccgaacc agaaacatta gatgtggtcc
    26701 cagaaaatat cccgttgacg atcgtttacg aagatgaaga tgtagcggtg ataaacaaac
    26761 ctcaaggaat ggtcgttcat ccttcagcag gtcatacgca tggcacaatg gtcaatgcgc
    26821 tgatgtacca tatcaaggat ctttctacga tcaacggcgt tattcgtcca ggtattgttc
    26881 accgaatcga caaagatact tccggactgc taatggttgc caaaaatgac caggcacacg
    26941 aatcattagc tcagcaatta aaagataaga cctctttgcg caaatatgtt gctttagtcc
    27001 atggagagat tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt tcgaaggaaa
    27061 accggaaaat gcaggcgata agagaagatg ggaagtcggc tgtcacacat tttactgtat
    27121 tggaacgttt cgaaggattt actctggttg aattaaaact agaaaccgga agaacacacc
    27181 agatccgtgt gcatatgaag tatatcggtt atccgttagc tggagatcca gtatacggac
    27241 cgaagaaaac tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta ggttttacgc
    27301 acccgactac aaacgaaaag atgatctttg aagcacctct tccagaagtt tttgaaaaaa
    27361 ctttagaaaa attaagaaaa aacattgcat tttaatttag ataacggtat agtaattaac
    27421 aacgaaagca taaaacttaa tagaattcct ttaagtttag tcccgtgagg ctaagaaggg
    27481 tgggagcaag gatacgtatg gtgcgccata agtagattgc tcattgacat agtatagcta
    27541 tcagtgtatc agtatacgct cagtctaaac attcctcctg tcctcttagg gcaggagttt
    27601 ttttatgcaa aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta gatcaggtaa
    27661 ccatgaagcg agcattgact cgaattactt atgagattat cgagagaaac catagtatcc
    27721 aagatatcgt attggtaggt atcaaaacac gaggaatcta cattgcatca agaattgccg
    27781 aacgcctgaa gcagctagaa gatatcgata ttcctgtagg agaactggat atcactttat
    27841 accgagatga caaaaaagaa aatccagaag aaccggaact tcattcttct gacatcccag
    27901 tttccttaga aggaaaagaa gttattttga ttgatgatgt tttgtataca ggacgaacga
    27961 ttcgagcagc aatggacgca gtaatggatt ttggcagacc aagaaaaatt tcattagcag
    28021 tcctggtcga tcgtgggcat cgagaattgc caatccgtgc agattatgtt gggaagaaca
    28081 ttccaacatc tcgggcggaa gagattttag tagagatgca agagcttgat ggacaagatc
    28141 gcatcatgat tttgaaagag gaagattaga ggagagagaa acatgtcgga gaaaaaagga
    28201 tttcataatg atgacgtggt gttagatatc cacgatcgcc caaccccagg acattgggta
    28261 ggattaagtt tacaacactt gttcacgatg ttcggagcaa ccgtgttagt ccccatctta
    28321 gtaggtatcg atccaggtat tgcgctagtc agttcaggac taggtacatt ggtgtactta
    28381 tttactacta aaggaaagat acctgcgtac ttaggaagca gcttcgcttt cattgcagcg
    28441 atgcagcttt tgatgaaaac agatggttat ccagcgattg cacaaggagc agtcacaact
    28501 ggattagtct atttgattgt tgcgctgatc atcaaaaaga tcggttcagc atggctggat
    28561 aaaatcttgc ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt gggacttgcg
    28621 gcaaatgcag caacaaatgc tatgtataat cagggcacgt atgatttcaa gtatattgct
    28681 gtagcgctga tcacattagg attaacaatt ttcttcaaca tgtggctaag aggttttctc
    28741 ggattgattc cgattttact aggtattatt agcggataca tcgtagcctt gctgtttgga
    28801 atcgtggata ctaagccaat cgtagacgct gcctggtttg ctgttcctaa ctttgagatt
    28861 ccctttgtac aatatgaacc aaagctatat cttggagcaa tcacaacaat ggcacccatc
    28921 gcttttgtga cgatgacaga gcatatcggc catcttatgg tattgaataa actaactaaa
    28981 cgtaatttct ttgaagaacc gggtcttcat aagacgttga caggtgatgg attagcacaa
    29041 atcgtggcag cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa cattggtgtt
    29101 ctggctatca cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt atttgcagtc
    29161 attttaggat tcgtaggaaa attaagcgct ttgattttaa gtatccctgg accagtcatc
    29221 tcaggaatca gttttatctt atttggagta attgccgcaa gtggaatgaa gattttaatc
    29281 gagaacaaaa tcgattttga taaaaagaaa aatctgttga ttgcttcagt tatcttagta
    29341 gtaggaattg gcggactcgt ctttgaagta ggcacattta cattgtcggc aatggcatta
    29401 gcaactgtac ttggcatcat tttaaatctt atcttaccag aaacttcacg aagcgaagaa
    29461 aactaaggag gaaacattat gatcatcaaa tcagaacgta tcagcttaaa gcatttattg
    29521 accgttgagg cacttactga tcaagaagtt atgggcttga tccgcagagg gcaggaattc
    29581 aagcaaggtg ctaattggac tccgcaaaag cgccagtact ttgctacaaa cctctttttc
    29641 gaaaatagta ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact agggctagaa
    29701 gtgattgaat ttgaagcaag cacaagctct gtccaaaaag gggaaacact atacgataca
    29761 gtactgacga tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg tgatgagaac
    29821 tattatgacg aactgataca aagtaaaaca atccagtgtt cgattatcaa tggaggagac
    29881 ggaagcggac agcatccaac ccaatgcttg cttgatttga tgaccattta tgaagaattt
    29941 ggtcattttg aaggtctgaa agttgccatc gtaggtgata tcactcactc tcgcgtagct
    30001 aaatccaata tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc aggtcctaga
    30061 gaatggtacg acgatgaatt cgaagtgtac ggacattata tgccattgga tgaactacta
    30121 gatcaagttg atgtcatgat gatgcttcgg gtgcaacacg aacgccatga tggaaaagaa
    30181 agtttctcaa aagaaggcta ccatcaggaa catgggttaa cagttgagcg tgcgaagaag
    30241 ttgcaaaaac atgcaatcat tatgcaccca gcaccagtta accgtgatgt agaattagct
    30301 gattcattag tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa tggagtattc
    30361 gtcagaatgg caatcttaga agcagtgcta aatggaaaag cgtaattata ggaggatgaa
    30421 aaaatgaaaa cactcattaa aaacggtcag atcaatacac gaaaaaatat gacgacacca
    30481 gcagaaattt ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt ctcagaagcc
    30541 gaatttgatg aagtgtttga tgcaaaggga caattgatca cacctggttt agtggacgtc
    30601 catgttcatt tgagagagcc aggatttaca tacaaggaga cgatcgaagc tgggactcgc
    30661 tcagctgctc gtggcggttt cactactgta tgcgcgatgc cgaatttaaa tcctgtacct
    30721 gatacggcag aaaagctgag acaagtatat gacatcatta gaaaagatgc agttgtgaag
    30781 gtattgcagt atgcgccaat cacagaaaat ctgcgaagtg agaaattagt tgatcaagaa
    30841 gcactgattg aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt ccaaacagct
    30901 gggacgatgt atctggctat gaaagaagct gcaaaaaaca ataaagccct agtagcccat
    30961 acagaagatg aatctttatt atttggcggc gttatgcacg ctggcaagaa ggcagaagaa
    31021 cttggtcttc ccgggatctt aagcgtaacc gagtcttctc aaattgctag agatctgttg
    31081 ctagctgaag caactggtgt tcattaccat gtttgccatg tttctactaa agaaagtgtc
    31141 cgtgtaatca gagacgcaaa aaaagcagga atccacgtaa cagcagaagt ttcaccccat
    31201 catcttattt tgatcgacga agatattccc gaagatttcg gcttttggaa aatgaacccg
    31261 ccgctaagag gacgggagga cagagaagct ttgatcgaag gcttgcttga cggtacgatc
    31321 gattgcatcg ctacagacca tgccccccat ggattagaag aaaaaagcca aagtttcatg
    31381 aaatcaccat ttggtatcgt aggaagcgaa acagcgtttc aactgatcta tactcatttt
    31441 gtagaaactg gtcgattcac gttggaacaa gtgatcaatt ggttggcagt taaaccggct
    31501 gagatttttg gtttgaatgc aggaacgcta acggttggtg cccccgctga tgtagcagtt
    31561 tttgatatta cacaaacttg cacgatcgat aaagaagact ttttatcaaa aggggagaat
    31621 acacctttta tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt caacggaaaa
    31681 cttgtgtggc agaagggaga ataaaagtat ggaacgtttg cttatattag aagatggaac
    31741 agttttcgaa ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa tcgtttttac
    31801 aacaagtatg actggttatc aagaaactat cactgatcaa agttttaacg gtcaaatcat
    31861 tacatttacc tatcctatgg tgggtaatta tggggtaaat agagatgatt atgaatcgat
    31921 ttctccaaca tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt caaactggcg
    31981 aaaccaaatg acgctagatt catttttgaa acaaaaagga attcctggaa tatcaggtat
    32041 cgatacgcgc gcccttactc gcaaaatccg tgaagtcgga acgatgaaag ctagtatcgt
    32101 tgatgctgga gattccttcg aacatgcatt tgatcagctg aaagcaaccg ttctagcaac
    32161 caatcaagtg cagcaagtct caactaacaa accttatcca agtccaggaa caggtcggaa
    32221 agtcattgtc attgacttcg gcttgaaaca tagtatttta cgagaattga gtaaaagaaa
    32281 ctgtaacctc acagtacttc cctataacac agatgccgag acgattttag cgctatcacc
    32341 agacggagtc atgctgacga atggacctgg tgatccaaaa gatgtgccag aagcaatcga
    32401 aatgatcaaa aaaatccaag gaaaagtacc gatttttggt atttgtctcg gtcaccagtt
    32461 atttgcttta gccaacggtg cagataccta taaaatgaaa ttcggacatc gaggattaaa
    32521 tcacccggta cgagaagtag cgaccggaag aattgatttt acttctcaaa atcatggcta
    32581 tgcagtcgat gaagccacga ttgatccaga aaaactcatc gtaactcatg tagaagtaaa
    32641 tgacggaaca gtagaaggag tcaaacacag agattatccc gctttcagtg tgcagtttca
    32701 tccagatgct gcccctggtc cgcatgatgc actacattta tttgatgaat ttatggaaat
    32761 gatggacgca ggaaaggagc agtaaacaga tgccgaaacg tacagatatc aagaaaatta
    32821 tggtgattgg atctggtccg atcatcattg gtcaagccgc agaatttgac tacgcaggaa
    32881 cacaagcctg ccttgcctta aaagaagaag gatatgaagt ggttttagta aattctaatc
    32941 ctgcgacgat catgacggat aaagaagtgg cagatcaagt atatatcgaa ccgatcacac
    33001 ttgaatttgt atcaaggatt cttcgaaaag agcgtccaga tgctctcctt ccaactttag
    33061 gcggacagac aggcctcaat atggcaatgg agctggcagc atcaggtatt ttagaagaat
    33121 tgaacataga acttctagga acaaagcttt ctgcgatcga tcaagcagaa gatcgtgatc
    33181 tattcaaaca gttgatgaaa gaattgaatc agccaatccc cgagtctgaa atcgtcaata
    33241 cagtagaaga agctgtagat tttgcaaata aaatcggcta tcctgtcatc gttcgtccag
    33301 cctttacttt aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa ttacgtatcg
    33361 tatcagaaaa tggattaagt ctttcccccg ctacccaatg tttgattgaa cgaagtattg
    33421 caggttttaa agaaattgaa tatgaagtga tgagagattc ggctgataat gcaatcgttg
    33481 tctgcaatat ggaaaatttt gacccggtgg gtatccatac aggtgattct atcgtatttg
    33541 cccctagtca aacattgtct gaccatgaat atcaaatgct tcgtgatgct tctctttcga
    33601 ttattcgagc acttaagatc gagggaggct gtaatgtcca actcgctctt gatccacata
    33661 gttttaatta ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct gcgttagcaa
    33721 gtaaggcgac tggctatccg atcgctaagc tagctgctaa aatagcgctt ggattaacat
    33781 tagacgaaat gaaaaatcct gttacaggaa cgacttttgc agaatttgaa cctgcattag
    33841 attatgttgt agctaaaatt cctcgctggc cgtttgataa gtttgaaaac ggagaacggc
    33901 ttttaggcac gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg aatatcgaag
    33961 agtctttatt gaaagctgtg cgttctttag aaatcggtac acaccatatc gaacttcctg
    34021 agttggcaga catcacggac gatgacctaa tccaaaaagt ggttcgagca caagatgatc
    34081 gcctgtttta tgtagctgaa gctattagaa gagggtacac catagaagaa ttagcggatt
    34141 tgacaaaaat cgatttattc tttttagata aactgttgca tatcgtagaa atcgaacagc
    34201 agttgaaaca gcagccgttg gatgacaaga tattgaaaac agcgaaacaa aacggtttta
    34261 cagacaaaaa agtggcggag ctgtgggaaa taaacgaaga agccgttcgc cagatgagaa
    34321 aagaaaatga tttgattcct gtatataaga tggttgacac ttgtgcagcg gaattcgaat
    34381 caaaaacacc ttatttttat agttcctatg aatatgaaaa tgaaagttca cgatcagaaa
    34441 agccctcaat tctagtactg ggatctggtc ctatccgtat cggacaaggg gttgaatttg
    34501 actatgcgac tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa gcaatcatca
    34561 tgaacagcaa tcctgaaaca gtttcgactg acttttctat ttcggataaa ttatattttg
    34621 agccattgac cttcgaagat gtgatgaatg tgatcgagct agaacagccg ataggagtaa
    34681 tcgttcaatt tggtggtcag acagcaatca acttagccga acctttgact gctgctggtg
    34741 taaaaatctt agggacaacg atcgaagact tggatcgtgc ggaaaatcgt gatttattcg
    34801 agcaagcgct gaaagagttg gatattcctc aacctttagg ggatactgct acaagtaaag
    34861 aagaagccgt ggttatcgct tcgagaattg gttatccagt tcttgttcgt ccaagctatg
    34921 tattaggcgg acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa gattacatgg
    34981 aacatgctgt gaaagcatct ccagaacatc cagtactagt ggaccgttac ttgcttggtg
    35041 gtgaatgcga agtggatgcg atttgtgacg gtgaaacagt actcatccca gggatcatgg
    35101 agcatattga gcgtgctggt gtccattcag gggattcaat ggctgtgtat ccacctcaaa
    35161 cattatctga atcgatcaag aaaacgatcg aagattacac gatccgctta gcattaggtt
    35221 tgaattgtgt cgggatgatg aatatccagt ttgtcatcca tgaaaatgag gtttatgtga
    35281 tcgaagtcaa tcctcgagca agtcggactg ttccattctt aagtaaggtg acaggaattc
    35341 caatggcaca aattgcgacg aaagctattt tgggagagaa actaaagaca cttggttatg
    35401 aaaacggact ttatccagaa acaaaagaag ttcatgttaa agcacctgtc ttttctttta
    35461 cgaaattgca gaaagttgat acttacttag ggcctgaaat gaaatcgact ggagaagtaa
    35521 tgggatctga cagacaatta gacaaagctt tgtacaaggc attcgaagct tctggattac
    35581 acttgcctga atatggagcc gtattattta cgattgcgga cgaaacaaaa gaagaagcac
    35641 tagaattagc taaacgtttt acagaaattg gctatagtct gataggaacg aaacatacag
    35701 ctgcttattt cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt tctgaggaag
    35761 cagcagaaaa aaatgtagtc gacttaataa gagatggtaa aacacaagtg gttgtcaata
    35821 cgattgataa agatcgggga aatgcttcta aagacggttt catcatccga agagaagcag
    35881 ttgaacatgg tattccattg tttacttctt tagacacagc agatgcgatc gttcgagtga
    35941 tggaatcccg tgctttttca actaacccta tttaacggcg agcacatgtt aagacaaaaa
    36001 gacgaaaaac cgttgcagga ggggacagat gaaacaagaa atgatggaaa tcgttcagca
    36061 aaaacagctg gcaccaaaga tttttgaaat gacattgaaa ggtgatctag taaaagaaat
    36121 gcagcaacca ggacagttct tgcatattct cgtgccgcgt gcagacttgc tattacgccg
    36181 accgattagc ttgaatcata tcgaccacga aacaaatact tgccgtatca tttatcgggt
    36241 ggaaggtgag gggactagag tcttctctac tctttctgcc ggagaccatt tagatgtcat
    36301 gggtccttta ggcaatggtt ttgatttgtc caatcttcat acaggagacg aagtatttat
    36361 aatcggtgga ggaatcggtg tccctcctct atatgagtta tcaaaacagt tagtcgaaaa
    36421 aggaattaag ccaacgcatt ttttaggttt tgccacacat gaagtcgttt attatgaaaa
    36481 agaatttaat cgtctaggag aaacaagaat cgctactgac gatggaacat acggtatgca
    36541 cggaaatgtc ggtaatttat tgttaggaac gaagacggaa cctacagctg tttttgcatg
    36601 tggttcaaac gggttattga agacagtgga acagctgttt tcttcgcatc aaaatgtcca
    36661 gttatcatta gaatcccgca tggcatgtgg gataggggct tgctatgctt gcgtttgtca
    36721 tacgccggaa gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag gaccgatttt
    36781 taaaatagga gaggtaatta tatgaatgag aatcctttag cggtcaaact tcctggccta
    36841 gaattaaaaa atcctatcat gccggcaagc ggctgtttcg gttttggcaa agaatacgga
    36901 aaatactatg atttgaatca gcttggatcg atcatggtca aggcaacgac tccaaatgcc
    36961 cgctttggca atcccacgcc aagagtggcg gaaacaccat ctggcatgtt gaatgcaatt
    37021 ggtttgcaaa atcctggtct agacgttgtt atgcataaac ttcttccaga attagaggag
    37081 tacaatgagt tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga ctatgtagaa
    37141 gtttgtagca aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa catttcttgc
    37201 ccaaatgtca aacatggcgg aattgctttt ggaacagatt ctgatgttgc ttttcaatta
    37261 acacaagcag taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc cccaaatgta
    37321 acagacatcg ttccaatcgc tcaggcaatc gaagctggag gggcagatgg gtttacgatg
    37381 atcaatactc ttcttggtat gcggattgac cttaagacaa gaaaaccgat tttagccaat
    37441 caaacaggtg gactctccgg accagcaatc aagcctgtcg ctattcgttt gattcaccaa
    37501 gtagcaagta tatccagcct tccaatcatt gggatgggcg gcgtgcagac agtggatgat
    37561 gttcttgaga tgtttatggc tggagcaagt gctgtagcta tagggaccgc gaatttcacc
    37621 gatccgtata tctgtcctaa actaattaaa gagctccctg tgcgaatgag cgaactaggc
    37681 atcgaatcgc tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg atgaatcaac
    37741 tagaccaacg accaattatt gcattagatt tttcaactag gcaagaggta gaagactttc
    37801 ttcgattttt cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa ttgttctacc
    37861 aagaaggacc tgaaatcgta cgttatctaa aagaggctgg tcataatgta tttttagatc
    37921 tgaaacttca cgatattccg aatacggtcg aaaaagcaat gcggggattg gcaaagcttg
    37981 gtgttgatct cacctgtgtt catgccgctg gcggtattcg tatgatggag gcggcaatgc
    38041 gaggattaga agaggggact ccagaaggtg gaaaacgtcc gctacttcta gctatcactc
    38101 agcttacttc tacaagtgaa gaagaaatgc atgccgatca actaatcgaa gtttctttag
    38161 aaaaaagtgt gatccattat gctagctgtg ccaaaaaagc aggattagat ggtgtggttt
    38221 cttctgcttg ggaagtagaa gcgatcaaag aaatggcagg agacgaattt gtttgcttaa
    38281 ctccgggtat aagaccagaa ggaacagtcg caggagatca gacaagagta gtcacacctt
    38341 ctcaggcaaa aaagatcggc tcaactttta tcgttatagg tcgaccaatc actcagtcaa
    38401 ctaatcctta taaagcatat caaacgattc aaacagaatg gagctaacct aaaatgaatg
    38461 ttgaacaaag tatcgcaaaa gacttattag aaattgaagc agtattttta aatccaagta
    38521 atccttttac atgggcaagt ggaatcaaaa gcccaattta ctgtgataac cgaatcacga
    38581 tgagctatcc aaaagtccga aaagagatcg caaaaggatt agcaagcaaa atcaaagaag
    38641 cttaccctga agtacaagtg attgctggaa ctgcaacagc aggaatccct catgccgcat
    38701 gggtagcgga aatactggat ttgccaatgg tctatattcg aagtaaggcg aaagatcatg
    38761 ggaaaggtaa ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt gtgatcgagg
    38821 atttgatttc tacaggcgga agtgtcttgg aagcagctga agcagcaaaa agagaaggtg
    38881 cggatatcct tggtgttgcg gctatcttta cgtatgagtt gccaaaagga aaagcaaatt
    38941 tcgaaaaagc cgaaatccct cttatgacgt tgaccaatta ttctgtattg attgaagcag
    39001 ctttagaaga tcgttatatc gatgagcaag aattaactct gctaaaagaa tggaaaaaag
    39061 atcctgaaaa ttggcagatt taagcgcgct tgatgaagat agagaaatcc gatttgtgtc
    39121 tccagaatca tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa gcatcttttc
    39181 atcaggtatg aaaagaaact tctatcgtga tcagtttttg atttttttaa aattcatttt
    39241 tctaagactg gttagcagaa agaagagaat aatctcttct cttttttcta ttatatatag
    39301 attttctcta ttttttggta agggagctgt ttcaaaaatg gtaaagagcg gaaatgttgc
    39361 gaatatcagt tatttaatga tatgatattc ggaaaaagaa atctcaaagg gaggtaactt
    39421 ttatggggtt gaatgaattt attttaggat tgaacaattt agagacgatc acaagggcac
    39481 ctggattttt taaatataca gagcatacag ttgcagcaca ttcgtatcgt gtagcatcta
    39541 ttgcgcaagt tctaggagac attgaagaag cgaatggaca accgatcgat tggaaactgc
    39601 tctatgaaaa atcattgaat catgactaca cagagcgttt tattggtgat atcaaaacgc
    39661 cggtgaaata cgcaagcaaa gagttaagag ggatgctgca aacagttgaa gaaaagatga
    39721 cggacgaatt catcaaccaa gagattccag ctgagttcca agaagtttat cgccgtcggt
    39781 tgttcgaagg aaaagatgaa accgtagaag gagaactatt agccatttca gataaggttg
    39841 accttcttta tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa gtttataagg
    39901 agatgttcat tgaggcagtg actacgatta aaagctttga acatcggcct tctgttcagt
    39961 atttctttaa agagatattt ccggaactat tgaaccagga attttatgga aaagaggcgt
    40021 ttttagaaac cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga tatgagatgc
    40081 agggggaagt atagttgaaa aagaatatgg atgtcgaatg gggctattct ggagacaaag
    40141 gtccggaaaa ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa tttccattgc
    40201 agtcgcctat tcatttgacc aagggggaag aaaccaaaat agaaggagac acgctttcct
    40261 ttcattatca aaagcaacgg tttactgaca aggaattcaa aaacacgatt catttagtac
    40321 cttttgacca actcagttat gtagagttca agaaagaacg ctattatttg acagatattc
    40381 atttccactt gccttctgaa catatcatca acggaaaaca ggaagatatt gaatttcatt
    40441 ttgttcatat gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac cagctaatgg
    40501 tacaggaagg ttggctgtat aatcaagaaa atggggaaag ttggaactta gaaaaacatg
    40561 agcattgggt aaatccggat gtgttctttc cggagcaaaa aagccatttc cattatattg
    40621 gctcattgac aactccgcct accagcgggc caatccaatg gtttgtaatg gatcatgtag
    40681 gaaaactgaa ccaagagttt cttctcccct tcgatggtca gtatgctcga ccgaacaacc
    40741 gaccaatcca gcccttgaat ggaagagaaa tttattattt cgaggagttt gagcaatgaa
    40801 tattcagcag atgaaatacg tggtcgctgt agcaaacaat ggtagtttca gagaagcggc
    40861 aaagaaaatg tttgtctccc agccaagtct ttcagctggt atcaaagaat tagaaaccga
    40921 gttagggatc acgctgttta ctcgaacgaa tcgaggggca tatttaacag aagaagggca
    40981 agacttctta aatcgtgccg agaaaatatt ggtccagctt gatacattag aaactcacta
    41041 tctaacgagt gaaaagagag acaatttttc gatcgctgcc cagcattatg atttcttagg
    41101 tcctttgacc gcacagctac tcgaggaatt caaagacgag atcaaacaat ttcggatcat
    41161 ggagacgaca acagcgagag tgatcgaaga agtcaaagag cagcacagtg agatcggcat
    41221 tatttatatg aatgagcata atcaagctgg tatcagaaga tatttacaac aaggagagct
    41281 aagtggccgc agcattggaa aattcaaaac gcatattttt ttaaggcaag gacatccatt
    41341 agctgaacac gcagagatca ccaaagaaga attgttatct tatcctcaag ttcgttttac
    41401 acaggatgga aataatttcc cgtattttta tgaagacctt atcgagatcc ctgatcaaga
    41461 aacagtgatt tatacgagtg atcgaggtac tttgatgaat atcgttcttg aaactgatgc
    41521 ttacgcttca ggatctggta ttgtgattgg agggattcgc gagcacttgc ggttaatccc
    41581 tttggcagaa ggcgaactaa atgaatttta tatcatttat tcggcgaaac gtccgctcag
    41641 tgaaatcgct caaagattta tcaaagagct tactcggtta ctggaccagc agagctaaga
    41701 aaagcagatg agaacgctgt ttattgaaca ccgttgatca aaaaagaggg gttgtgacag
    41761 aattt
//
LOCUS       DAFCLG010000011        40642 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFCLG010000011 DAFCLG010000000
VERSION     DAFCLG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.011"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            336..1439
                     /locus_tag="LND56_000594"
     CDS             336..1439
                     /locus_tag="LND56_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6742541.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="LND56_000595"
     CDS             1723..2403
                     /locus_tag="LND56_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6742542.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="LND56_000596"
     CDS             2493..3527
                     /locus_tag="LND56_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6742543.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="LND56_000597"
     CDS             3541..3876
                     /locus_tag="LND56_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742544.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="LND56_000598"
     CDS             3860..6340
                     /locus_tag="LND56_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742545.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="LND56_000599"
     CDS             6396..7955
                     /locus_tag="LND56_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742546.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="LND56_000600"
     CDS             7952..9316
                     /locus_tag="LND56_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6742547.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="LND56_000601"
     CDS             9487..10557
                     /locus_tag="LND56_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742548.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="LND56_000602"
     CDS             10572..11240
                     /locus_tag="LND56_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742549.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="LND56_000603"
     CDS             11260..11838
                     /locus_tag="LND56_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6742550.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="LND56_000604"
     CDS             12241..13152
                     /locus_tag="LND56_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6742551.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="LND56_000605"
     CDS             complement(13408..14325)
                     /locus_tag="LND56_000605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBK6742552.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="LND56_000606"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="LND56_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK6742553.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15552
                     /locus_tag="LND56_000607"
     CDS             15022..15552
                     /locus_tag="LND56_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBK6742554.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            15684..18044
                     /locus_tag="LND56_000608"
     CDS             15684..18044
                     /locus_tag="LND56_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK6742555.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18131..18445
                     /gene="trxA"
                     /locus_tag="LND56_000609"
     CDS             18131..18445
                     /gene="trxA"
                     /locus_tag="LND56_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBK6742556.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18627..20405
                     /gene="uvrC"
                     /locus_tag="LND56_000610"
     CDS             18627..20405
                     /gene="uvrC"
                     /locus_tag="LND56_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK6742557.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20550..21449
                     /locus_tag="LND56_000611"
     CDS             20550..21449
                     /locus_tag="LND56_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742558.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21442..22704
                     /locus_tag="LND56_000612"
     CDS             21442..22704
                     /locus_tag="LND56_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742559.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22867..24765
                     /locus_tag="LND56_000613"
     CDS             22867..24765
                     /locus_tag="LND56_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBK6742560.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25064..25783)
                     /locus_tag="LND56_000614"
     CDS             complement(25064..25783)
                     /locus_tag="LND56_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBK6742561.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26120..26452
                     /locus_tag="LND56_000615"
     CDS             26120..26452
                     /locus_tag="LND56_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742562.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26439..28442
                     /locus_tag="LND56_000616"
     CDS             26439..28442
                     /locus_tag="LND56_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBK6742563.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28461..28931
                     /locus_tag="LND56_000617"
     CDS             28461..28931
                     /locus_tag="LND56_000617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBK6742564.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28952..29536
                     /locus_tag="LND56_000618"
     CDS             28952..29536
                     /locus_tag="LND56_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBK6742565.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29551..30537
                     /locus_tag="LND56_000619"
     CDS             29551..30537
                     /locus_tag="LND56_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBK6742566.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30527..30838
                     /locus_tag="LND56_000620"
     CDS             30527..30838
                     /locus_tag="LND56_000620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBK6742567.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30860..32641
                     /locus_tag="LND56_000621"
     CDS             30860..32641
                     /locus_tag="LND56_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBK6742568.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32634..34010
                     /locus_tag="LND56_000622"
     CDS             32634..34010
                     /locus_tag="LND56_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBK6742569.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34017..34652
                     /locus_tag="LND56_000623"
     CDS             34017..34652
                     /locus_tag="LND56_000623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBK6742570.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34682..36037
                     /locus_tag="LND56_000624"
     CDS             34682..36037
                     /locus_tag="LND56_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBK6742571.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36229..37623)
                     /locus_tag="LND56_000625"
     CDS             complement(36229..37623)
                     /locus_tag="LND56_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6742572.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37715..38686
                     /locus_tag="LND56_000626"
     CDS             37715..38686
                     /locus_tag="LND56_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBK6742573.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38683..39465
                     /locus_tag="LND56_000627"
     CDS             38683..39465
                     /locus_tag="LND56_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBK6742574.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39634..40005)
                     /locus_tag="LND56_000628"
     CDS             complement(39634..40005)
                     /locus_tag="LND56_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6742575.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40139..40582
                     /locus_tag="LND56_000629"
     CDS             40139..40582
                     /locus_tag="LND56_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBK6742576.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
BASE COUNT        13427 a         6919 c         8650 g        11646 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15601 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15661 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15721 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15781 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15841 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    15901 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15961 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    16021 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    16081 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    16141 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    16201 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    16261 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    16321 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    16381 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16441 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    16501 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16561 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16621 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16681 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16741 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16801 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16861 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16921 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16981 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    17041 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    17101 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    17161 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    17221 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    17281 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    17341 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    17401 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17461 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17521 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17581 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17641 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17701 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17761 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17821 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17881 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17941 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    18001 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    18061 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    18121 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    18181 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    18241 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    18301 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    18361 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18421 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18481 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18541 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18601 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18661 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18721 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    18781 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18841 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18901 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18961 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    19021 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    19081 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    19141 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    19201 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    19261 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    19321 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    19381 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19441 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19501 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19561 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19621 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    19681 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19741 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19801 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19861 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19921 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    19981 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    20041 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    20101 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    20161 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    20221 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    20281 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    20341 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    20401 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20461 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20521 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20581 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20641 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20701 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20761 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20821 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20881 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20941 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    21001 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    21061 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    21121 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    21181 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    21241 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    21301 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    21361 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21421 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21481 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21541 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21601 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21661 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21721 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21781 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21841 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21901 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    21961 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    22021 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    22081 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    22141 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    22201 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    22261 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    22321 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    22381 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    22441 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22501 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22561 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22621 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22681 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    22741 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    22801 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    22861 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    22921 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    22981 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    23041 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    23101 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    23161 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    23221 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    23281 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    23341 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    23401 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    23461 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    23521 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    23581 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    23641 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    23701 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    23761 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    23821 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    23881 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    23941 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    24001 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    24061 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    24121 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    24181 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    24241 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    24301 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    24361 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    24421 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    24481 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    24541 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    24601 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    24661 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    24721 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    24781 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    24841 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    24901 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    24961 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    25021 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    25081 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    25141 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    25201 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    25261 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    25321 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    25381 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    25441 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    25501 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    25561 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    25621 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    25681 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    25741 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    25801 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    25861 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    25921 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    25981 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    26041 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    26101 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    26161 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    26221 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    26281 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    26341 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    26401 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    26461 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    26521 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    26581 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    26641 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    26701 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    26761 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    26821 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    26881 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    26941 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    27001 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    27061 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    27121 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    27181 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    27241 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    27301 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    27361 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    27421 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    27481 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    27541 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    27601 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    27661 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    27721 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    27781 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    27841 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    27901 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    27961 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    28021 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    28081 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    28141 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    28201 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    28261 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    28321 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    28381 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    28441 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    28501 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    28561 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    28621 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    28681 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    28741 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    28801 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    28861 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    28921 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    28981 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    29041 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    29101 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    29161 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    29221 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    29281 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    29341 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    29401 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    29461 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    29521 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    29581 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    29641 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    29701 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    29761 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    29821 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    29881 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    29941 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    30001 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    30061 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    30121 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    30181 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    30241 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    30301 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    30361 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    30421 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    30481 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    30541 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    30601 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    30661 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    30721 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    30781 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    30841 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    30901 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    30961 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    31021 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    31081 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    31141 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    31201 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    31261 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    31321 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    31381 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    31441 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    31501 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    31561 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    31621 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    31681 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    31741 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    31801 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    31861 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    31921 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    31981 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    32041 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    32101 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    32161 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    32221 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    32281 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    32341 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    32401 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    32461 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    32521 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    32581 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    32641 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    32701 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    32761 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    32821 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    32881 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    32941 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    33001 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    33061 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    33121 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    33181 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    33241 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    33301 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    33361 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    33421 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    33481 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    33541 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    33601 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    33661 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    33721 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    33781 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    33841 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    33901 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    33961 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    34021 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    34081 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    34141 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    34201 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    34261 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    34321 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    34381 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    34441 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    34501 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    34561 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    34621 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    34681 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    34741 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    34801 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    34861 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    34921 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    34981 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    35041 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    35101 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    35161 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    35221 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    35281 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    35341 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    35401 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    35461 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    35521 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    35581 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    35641 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    35701 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    35761 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    35821 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    35881 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    35941 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    36001 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    36061 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    36121 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    36181 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    36241 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    36301 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    36361 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    36421 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    36481 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    36541 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    36601 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    36661 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    36721 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    36781 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    36841 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    36901 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    36961 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    37021 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    37081 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    37141 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    37201 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    37261 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    37321 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    37381 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    37441 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    37501 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    37561 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    37621 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    37681 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    37741 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    37801 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    37861 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    37921 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    37981 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    38041 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    38101 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    38161 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    38221 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    38281 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    38341 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    38401 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    38461 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    38521 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    38581 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    38641 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    38701 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    38761 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    38821 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    38881 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    38941 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    39001 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    39061 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    39121 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    39181 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    39241 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    39301 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    39361 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    39421 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    39481 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    39541 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    39601 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    39661 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    39721 agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    39781 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    39841 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    39901 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    39961 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    40021 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    40081 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    40141 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    40201 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    40261 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    40321 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    40381 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    40441 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    40501 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    40561 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta
    40621 tttattacag tacatatttt tt
//
LOCUS       DAFCLG010000012        39817 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFCLG010000012 DAFCLG010000000
VERSION     DAFCLG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.012"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..983)
                     /locus_tag="LND56_000630"
     CDS             complement(<1..983)
                     /locus_tag="LND56_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6742577.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEK"
     gene            complement(993..1556)
                     /gene="ahpC"
                     /locus_tag="LND56_000631"
     CDS             complement(993..1556)
                     /gene="ahpC"
                     /locus_tag="LND56_000631"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBK6742578.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(1700..2362)
                     /locus_tag="LND56_000632"
     CDS             complement(1700..2362)
                     /locus_tag="LND56_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6742579.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            2546..4192
                     /locus_tag="LND56_000633"
     CDS             2546..4192
                     /locus_tag="LND56_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK6742580.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4183..5265
                     /gene="uxuA"
                     /locus_tag="LND56_000634"
     CDS             4183..5265
                     /gene="uxuA"
                     /locus_tag="LND56_000634"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6742581.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5373..6926)
                     /locus_tag="LND56_000635"
                     /pseudo
     CDS             complement(5373..6926)
                     /locus_tag="LND56_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7074..8215
                     /locus_tag="LND56_000636"
                     /pseudo
     CDS             7074..8215
                     /locus_tag="LND56_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8341..9420)
                     /locus_tag="LND56_000637"
     CDS             complement(8341..9420)
                     /locus_tag="LND56_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6742582.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(9529..9969)
                     /locus_tag="LND56_000638"
     CDS             complement(9529..9969)
                     /locus_tag="LND56_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6742583.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10026..10436)
                     /locus_tag="LND56_000639"
     CDS             complement(10026..10436)
                     /locus_tag="LND56_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6742584.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(10617..11051)
                     /locus_tag="LND56_000640"
     CDS             complement(10617..11051)
                     /locus_tag="LND56_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6742585.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(11151..11999)
                     /locus_tag="LND56_000641"
     CDS             complement(11151..11999)
                     /locus_tag="LND56_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6742586.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12388..14154
                     /locus_tag="LND56_000642"
     CDS             12388..14154
                     /locus_tag="LND56_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBK6742587.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14256..15515
                     /locus_tag="LND56_000643"
     CDS             14256..15515
                     /locus_tag="LND56_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6742588.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            15662..16963
                     /locus_tag="LND56_000644"
     CDS             15662..16963
                     /locus_tag="LND56_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6742589.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            16966..17817
                     /locus_tag="LND56_000645"
     CDS             16966..17817
                     /locus_tag="LND56_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6742590.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            17830..18645
                     /locus_tag="LND56_000646"
     CDS             17830..18645
                     /locus_tag="LND56_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBK6742591.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            18796..19962
                     /locus_tag="LND56_000647"
     CDS             18796..19962
                     /locus_tag="LND56_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6742592.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20192..20839
                     /locus_tag="LND56_000648"
     CDS             20192..20839
                     /locus_tag="LND56_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6742593.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            20923..21867
                     /locus_tag="LND56_000649"
     CDS             20923..21867
                     /locus_tag="LND56_000649"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBK6742594.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22002..23453
                     /locus_tag="LND56_000650"
     CDS             22002..23453
                     /locus_tag="LND56_000650"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK6742595.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            23474..24310
                     /locus_tag="LND56_000651"
     CDS             23474..24310
                     /locus_tag="LND56_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6742596.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24310..24969
                     /locus_tag="LND56_000652"
     CDS             24310..24969
                     /locus_tag="LND56_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6742597.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25095..25784
                     /locus_tag="LND56_000653"
     CDS             25095..25784
                     /locus_tag="LND56_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742598.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            25790..26746
                     /locus_tag="LND56_000654"
     CDS             25790..26746
                     /locus_tag="LND56_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBK6742599.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            26790..27302
                     /locus_tag="LND56_000655"
     CDS             26790..27302
                     /locus_tag="LND56_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742600.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(27558..28106)
                     /locus_tag="LND56_000656"
     CDS             complement(27558..28106)
                     /locus_tag="LND56_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6742601.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28195..29376)
                     /locus_tag="LND56_000657"
     CDS             complement(28195..29376)
                     /locus_tag="LND56_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK6742602.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(29612..31120)
                     /locus_tag="LND56_000658"
     CDS             complement(29612..31120)
                     /locus_tag="LND56_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBK6742603.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31279..31470
                     /locus_tag="LND56_000659"
     CDS             31279..31470
                     /locus_tag="LND56_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742604.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            31673..32590
                     /locus_tag="LND56_000660"
     CDS             31673..32590
                     /locus_tag="LND56_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBK6742605.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            32583..33656
                     /locus_tag="LND56_000661"
     CDS             32583..33656
                     /locus_tag="LND56_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742606.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            33804..34322
                     /locus_tag="LND56_000662"
     CDS             33804..34322
                     /locus_tag="LND56_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742607.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34303..34695
                     /locus_tag="LND56_000663"
     CDS             34303..34695
                     /locus_tag="LND56_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBK6742608.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            34771..35706
                     /locus_tag="LND56_000664"
     CDS             34771..35706
                     /locus_tag="LND56_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBK6742609.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            35839..36618
                     /locus_tag="LND56_000665"
     CDS             35839..36618
                     /locus_tag="LND56_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6742610.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            36611..36928
                     /locus_tag="LND56_000666"
     CDS             36611..36928
                     /locus_tag="LND56_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6742611.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37172..38782
                     /locus_tag="LND56_000667"
     CDS             37172..38782
                     /locus_tag="LND56_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6742612.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            38939..39787
                     /locus_tag="LND56_000668"
     CDS             38939..39787
                     /locus_tag="LND56_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6742613.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13068 a         7120 c         7695 g        11934 t
ORIGIN      
        1 tctttttcgc ctggtacatt gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt
       61 tttgctttaa gtgatatacc gttagctaag ccaatattaa taaaatgttg ctctttattt
      121 aaggaaacgg cctgttgatt catcataatg tcgatagggt aactacgcac atgactttct
      181 acttgctgca tcaattttgg accttctgtg taagaggtac caatcatatt ttcaatccct
      241 agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa gtcctgtacg gattccttta
      301 cgtgctgcat agatagctgc actactagct gctggtccac cgccaatcac taatacatca
      361 taaggttctt tttcaaacaa ctcttcttca atcaaagggc cagtgatttt ttcaacaatt
      421 tgttcgatcg tcatgcgtcc gctactaaat tcttctccat ttaaaaacac agttggcact
      481 gccatgattt ttttatcatc gatttctttt tggaacatcc caccttcaat catcgtatga
      541 gtaatatttg gatttaatac agctaatata ttcaatgcct gtacaacgtc ggggcaatta
      601 tgacaagtca agctaacata actgtcaaaa tgcaatgttt gtgttatttt tttgatttta
      661 agactcaaag attcactgat tttcggtgcc cgtccactaa tttgtaacat agccagtaaa
      721 aaagaggaaa attcatgacc gagggggata ccagcaaaaa cgattccact ttctttgcca
      781 actggattga tagaaaaact aggtgtgcgc atcgaatctc ttttttccca agagatttta
      841 ggagacattt ctgctatttc cttcgtaaat tcagctagtt tttgactatt ttcactatta
      901 tccaaactaa cagaaaaaac aataggagat tcaatcagtg ctaaatattg ttcgagattt
      961 tgtttagttt ctttgtctaa catatccacc acctaaatct ttccaacaag atcaaaactt
     1021 ggtttcaatg tttcgccact ttctttccat tttgccggac aaacttcacc tgggtgggta
     1081 cgtacatatt gagcagcgcg aatcttatca atcagtgcac tagcgtcacg accgattcca
     1141 tctgcattga tttccatagc ttgaacgatt ccgtctggat caataataaa tgttcctcgc
     1201 tgagctaatc cttgctcttc atccaaaaca tcaaaaatac gagaaatctg atgagacgga
     1261 tcaccgatca tcacgtattc gattttaccg atagcatctg atgtatcatg ccatgctttg
     1321 tgagtaaagt gggtatcggt agagcaagaa aagacttcaa cacctaaaga tttcaatgta
     1381 ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa caaaagtaaa atcagctgga
     1441 tagaagcaaa gaatactcca gttccccata agatcttttt cactgacttc taaaaattct
     1501 ccttgatgat aagcatgtgc attaaattct tcgatttttt tcccaatcaa tgacatagta
     1561 aaacctccaa taataattat ttatttataa ttattgtaat ttagttggag ttgcaaagtc
     1621 aatggatatt ttgttattta ataaagatta ctcaaaaacg aaggttaatt ataaaaatag
     1681 aagaggtcgc aagaaaatgc tactgctgga aatactcggg ataacgttga gtaacagttt
     1741 cctgttcttt cagcatcatg tgtaagtgca atcccgtttg aaatcgagct tcttctggtt
     1801 ttttatcaac taaagccttg agtaaatcat aatgttgttc catgatcgtt tcccatggaa
     1861 gcccctgaat ggtcactcgt aaccagcgga aacgttctaa atgggtatta tggtcatcta
     1921 accagttcca aacttctttt cttcctgcaa tttcgaaaca agcttcatga aataaattat
     1981 ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc tttttcttgt tcttctaaaa
     2041 tcgtttctag cactattcgt gtttgtgtgt ccaatttgga acaacattca atcatgattt
     2101 gctcttccaa ctgttctcgg acaaagcgag cattttctgc agaaacaaga ttaattttgg
     2161 aaacatacgt accactttga ggtatcgtat acacgagttc ttgctgacgt agttgaatca
     2221 atgcttcgcg aatgggagtc cggccgatag caagatgttc ctccagtgcc ttttcagata
     2281 ttttccttcc aggttctaag tcagcataga taatttgttt acgaatcgct tgataagctt
     2341 gtgattgcaa gctgcttttc atctttattc ctccaaaatc atttctatga acgtagtata
     2401 acagaagagg tctgaaaaac aatctggtat gtcacattaa ctagtaaatt tgtataaata
     2461 gtaaaatttt ggttgacata gcgattttta ttgcataaaa ttcacttgaa tacaaaaact
     2521 aatatgttag tttaggaggg gaaaaatgat tgatttaaac aactggagga actttatccc
     2581 tgaattatct caaaaagaag tagaaatacc tcattttgac cgtgcatcat taagagaaaa
     2641 tggacaaaaa aatcctgtct ggatccattt cggtggcgga aatctttatc gaggatttca
     2701 tgcagaaatc gctcaacgtt tagcggatct aggcgaattg tccactggtg tcattgtttg
     2761 tgaaacattc gatgaagaaa taattgaaag ggcttaccaa gattttgatg atttgattct
     2821 tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt atattagctg cgacagctga
     2881 cagtctgtat tgtcatccag attgttctga acactttgat aagatgattc gacactttga
     2941 aaatcctagt ttacaattag ttacttttgc tattactgaa aaaggttatc gattgaaaga
     3001 tacaaataat aaatatatag gaccgattgc agtcgatata aaaaatggtc caaagaagcc
     3061 atcacataca atgagtattc ttgctgtgct actttataaa cgttttttat ctggagcttg
     3121 gccgattgca cttgtctcta cagataattt ttcacaaaac ggtagtcaat tacgtacttc
     3181 tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat gtacaaaata catttatcca
     3241 atacttatcg aatgaagaaa ttgttgcgtt tccatggacg atgattgatc gtatcacccc
     3301 gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat
     3361 tttccaaacc gcaaaaggaa ataatattgc tccatttgtc aatacagagg acatccatta
     3421 tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt
     3481 cttgactgat cgtgcaacgg tagataaagc agatacgatg aaggtgacga cttgccttaa
     3541 tcctttgcat acgtctttag ctattatggg ttgtttactg gggtatagga gcatagctga
     3601 ggagatgaaa gatccagatt tatcgctgct agtttctgaa atcgggtaca atgaaggcct
     3661 cccagttgtg gagtctccag gtatcattga tccaaagatc tttctggaag aagtaattac
     3721 tgtgcgcttt tctaatccaa atattcctaa tatgcctcag cgaatcgcca cggatacctc
     3781 tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa tatatggaaa tagaagttaa
     3841 agacccgcag acacttgtat tcataccatt gactctcgct gcttggcttc gataccttat
     3901 tggcattgat gatcaaggaa aaccattcat cccgagtcca gacccactat tgacagacct
     3961 tcagcataag ctttccgata ttcaactggg tacgcaagat acatcacata ttcatgagaa
     4021 aatggctcct attttgtcaa ataaagaaat ttttggtgta gatctttacc aagcagagct
     4081 tggcagaaaa atagaagaaa tgttcatcaa gatggtagca ggaccaaatg ccgtacgaaa
     4141 aacattacag gattacttac aagaacatgg aggaaattta tcatggaaat gacttttaga
     4201 tggtacggat cacaggaaga aaagattacc ttagatgaaa tcagacagat tcctgcggtc
     4261 aaagggattg taggcacatt gatggatata ccagtaggag aggtatggcc tagggagagg
     4321 atcaaagaac taaaagaaga aattgagtct cgtgggttaa cactaaaagt aattgaaagc
     4381 gtcaatgtgc atgatgccat taaaattgga ttaccttccc gtgagcagta tatcgaaaac
     4441 tataagcaga caatccgaaa cttggcagaa tatggaatag aagtcatctg ctataatttt
     4501 atgcctgtat ttgactgggt gaaatctgac cttgactacc gcttagaaga cggctcatcc
     4561 acgttagctt ttatatctga tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc
     4621 gaacattcca gcaatgaatt tggattgccg ggatgggaac ctgagcgctt agctcctatc
     4681 aaatccttgc ttgaagccta tgcatcagta gacgaagaaa aacttcgaga aaattttgcc
     4741 tattttctcc aatcaatcat cccaacatgt gaagaggtag gggtcaaaat ggctgtacat
     4801 ccagatgatc cgccatactc gatttttgga ttagctcgaa tcgtaaagaa tcgagaagat
     4861 ttagattggt tgtgtcgcat agtagacagc ccttataacg gaatcactct ttgtacaggg
     4921 agtattgcag aagatccaga aaatgatgta tatgggattt tagcagaatt ttgccaaaga
     4981 gatcgaatcc catttgcaca tgttcgaaat attaaatttt tagctaatcg agatttctat
     5041 gaggcaccac ataaatccga atacggttca ttagacatgt accgcatact aaaaacaatg
     5101 tatgacaacg gatttaacgg atacatccgt cctgatcatg gaagaatgat ctggaatgag
     5161 acaggaagac caggatacgg cttatatgat cgagcattag gtgctgttta tctaaatgga
     5221 ttatgggaag caatcgaaaa aggaaacaaa agtaaagata attagtgaaa aaaactgtaa
     5281 ttttattatg taaataagaa ggatgttcaa attcttgata cataaatccg gaggttgtta
     5341 ataagcattc ggatttatgt atcttatttg gtctaggaag taacttgatt ttcttttttc
     5401 gattgatcca tgagtgcttc ttcttttaat tcaaagatat attctgagat cctcataatc
     5461 acttttttcc agtcttctgg tgtgatgatt ccttttaaaa tatagatttt tttgatatga
     5521 gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt tgcagttctc cataaaatca
     5581 ttttgtattt tgatcgattc atttgaaaaa atttgttcta tcttaacgat cagatcttta
     5641 ctgatataaa aatcacgcgt atagttcaca ccaatcacaa ttcgcggctg ttttttgtag
     5701 cgatcataca aatgtactag gtcttcaacc atccattgca cattttcctt ttctaaaata
     5761 actggtaaca agtgttgatt ttgttctctg aaatccatat aaaattgttc aatggcggtt
     5821 tggacttctt tcaatacgct atgttcttga taaagcatga aagtaggaag gggcgtattt
     5881 ggttgattga agttaagaaa tccattataa agtttatttc gtgcgaatgc caatgagtaa
     5941 agaatctttt ctttttcagg tataaacaaa tcaaatacag tacaaaggga ctccaccatt
     6001 tctcttgaat aaagaacatg aggaacgttt gcttttttta aaagtttgta ttgctccaag
     6061 cttgtttctt ccgattcgat tttcgtgata gaaagtcgag ctaaaatatt cactaagatg
     6121 cgttgttcgc gacaaattgg cagattaggt cgcaaaatat ctacagagag atcaataaaa
     6181 aagtcaatat attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga
     6241 aaattctctt tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt
     6301 cggtcaagta cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct
     6361 tcatcataaa attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca
     6421 tattgaattt gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata
     6481 gacaaaccgt actatgctaa aaatgcatta atctttttca ttttcgtata caaataagag
     6541 gcactatagt ttgtttgctg actgaaactg ataatcgaat ttgtctgaca gctgaacaaa
     6601 gaagtcaaga tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag
     6661 cagtaagccg gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata
     6721 tgcagttctg ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa
     6781 aaatttgatt cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga
     6841 tgttcagata ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagataaac
     6901 gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac
     6961 ttgaaaatat tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag
     7021 aatctctact atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat
     7081 attcaaccat cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga
     7141 cagtccacac agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca
     7201 atcgaattac ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa
     7261 aaggacaaac agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac
     7321 tacaatcagc tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga
     7381 acactttgac tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg
     7441 acttgttaaa accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc
     7501 atttaacgcc gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag
     7561 gagatcttgt tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc
     7621 ctacagaaaa tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata
     7681 cagaaactca accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac
     7741 cagatgtaac tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc
     7801 cagacacaga taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac
     7861 cagatgtgac tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa
     7921 acaaaaaaac aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc
     7981 taaaaataaa tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag
     8041 ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca
     8101 gctaccaaaa tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt
     8161 aagtatagca acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt
     8221 gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa
     8281 ataccagatt gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt
     8341 ttaagggata tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc
     8401 taaaaagaag gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc
     8461 gctaacaagt aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa
     8521 cttcggattc agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata
     8581 aacaacatct cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat
     8641 gataacacct gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag
     8701 ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac
     8761 aacaaattga gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta
     8821 aatatcttga aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt
     8881 ttttttcttt tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc
     8941 catttttcct atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc
     9001 aaaaaaatcg tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt
     9061 tggctgatgc tcgtaaaaat caataacgga ctggatcata tcaaatgttt gatgaataag
     9121 catcgggata tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat
     9181 gatagctaaa acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat
     9241 catcaaataa gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa
     9301 aagaacaaaa acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa
     9361 acgctgataa acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat
     9421 tatatgatgg aatatgaaaa taagaaagag aaagagcaaa aagaaaaacc gctaaatgac
     9481 gttatcttat gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt
     9541 ctggtcttga tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc
     9601 ttcctgttca agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg
     9661 caacgcagca ccttgaggag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg
     9721 ctgacgatta atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga
     9781 atccagacac aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac
     9841 taacatcaca agatactggg tataggtaag attcagcggc ttgaggaggg aagtgtagtg
     9901 tctaatgatt tccttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag
     9961 atcttccatt tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa
    10021 aaagtctatt cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa
    10081 tacggacaca ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct
    10141 tcgatagatc cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg
    10201 gaaacttgtg cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct
    10261 aatgcactgt taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca
    10321 ttttcgacgc gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatga
    10381 acttctccag agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa
    10441 ttaccacctt taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca
    10501 aagaaatgga ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg
    10561 aatagcaaac atatacaaaa aaatatcaga gacaaaaact aatctcatca taaacattac
    10621 atatatttct caaaaagttg ttttctttct tcaaaactta cttcacgatg gacctgatga
    10681 agcatccata aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc
    10741 atttctgtca tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc
    10801 tcgatatcag agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca
    10861 gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc tcgtgccata caaggtaaaa
    10921 atgacttcat tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag
    10981 attgtttgat acagttctag tgctttcaaa ctatcattta atacaaaatc tatttccgtt
    11041 cctttatgca taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta
    11101 taccagttaa agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt
    11161 ggatgataag taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca
    11221 cttggatcga tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg
    11281 atcaataaaa cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg
    11341 ccacgtccaa tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg
    11401 accataacag atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg
    11461 attgcaatag ataaaatagc ataaagaaac tcttctattc caaaaacaaa cagattaaga
    11521 gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt
    11581 ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc
    11641 gcaactccta aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta
    11701 atcattgttt ctggaactat tgcaatgatt aaaggaaaaa acaagcttcc gaaaagtact
    11761 ttaaaaaagg gttttttacc taaaaataga agagctagta ccaacataac gatattcaac
    11821 actaaaataa ctatagctcg gtcaaatcct aaaacagctt ccagtaatat tcctagaccg
    11881 ctgacacctc cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat
    11941 atgaagaggg caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt
    12001 tatccacttc cttaattcat taataatact cttatcttat tataacttac ttttcacctt
    12061 aagttaactt taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct
    12121 agaatatact tctacgttaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat
    12181 tgatcgctta agataaattc aggacactaa atagtacagg aagtagatta aaacttttaa
    12241 aagttttaag tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg
    12301 ttaccgataa catttttgaa agcgttgtct tttgtttctc tatcacttat agtgatgacg
    12361 tcatattaaa acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa
    12421 agtgaatatg catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa
    12481 aatgatatca aaaaagtata tgtgctttgt ggagatccat ataccataac aactgaaaaa
    12541 tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg
    12601 gaaattgaag tgacgagcga aacaaggcgc ttacagtatg cgttccatgt tgttggggaa
    12661 gataacacgg actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt
    12721 actgaaccaa atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt
    12781 ccagaatggg taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg
    12841 gacaaagcaa atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa
    12901 gacttcttcg ggggagattt gcaaggtgtg agggatcatt tggattactt gacggacctt
    12961 ggcgtcaatg gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat
    13021 acgattgatt attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg
    13081 attgatgaag cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg
    13141 ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa
    13201 gactggttcc acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa
    13261 actgctgaaa atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat
    13321 acagcaaatc cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa
    13381 ttcgatattg atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa
    13441 aaattcagag aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg
    13501 cattcctctc aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattc
    13561 acagattcga ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt
    13621 ggaatgaatc atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta
    13681 ttagattctc atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg
    13741 aagtctgtga tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca
    13801 gaaataggga tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag
    13861 gaggaacaag atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag
    13921 ttgtctaaga ttatttctga aggttctact cagtggctaa tagtatatga tcgtgaagat
    13981 aagctctatt tcacacggga actagaggga cagatcatct atgtttactt taatcagtca
    14041 aaagaacctt gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg
    14101 ttagaagacg ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg
    14161 ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt
    14221 gtaacagcac gttgaataca caggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt
    14281 tgggtttagg gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta
    14341 actcttcagc agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg
    14401 gatataaaga ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa
    14461 tcaagctagt agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg
    14521 ctggaaaggg acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc
    14581 aaggacatct ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag
    14641 cccaagtgac ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt
    14701 tgtactataa caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc
    14761 tatccaaaga cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag
    14821 ctaaatggac tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat
    14881 ttggtgatga tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag
    14941 gaatctctta tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa
    15001 aaagtgcagg agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg
    15061 acggtccatg gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa
    15121 ttcctactct aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag
    15181 tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc
    15241 aggaaaatca agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta
    15301 gggaaacagc aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa
    15361 cagctcaagc gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact
    15421 tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa
    15481 aaacaatcag tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta
    15541 taaaaaatgg aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg
    15601 ctcttgttgc cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca
    15661 gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat
    15721 tccagggatg gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat
    15781 attcgtccta ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt
    15841 tactttggga agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt
    15901 gcaattcatc attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta
    15961 tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat
    16021 acataatata ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat
    16081 gatttttacg atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga
    16141 cttcaatcat attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag
    16201 tatcttcttt ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat
    16261 ttggacttta tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc
    16321 tcaccagaaa tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc
    16381 ggtcccagct tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc
    16441 catcaatact caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat
    16501 ctcttggaaa acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt
    16561 aggatttccc tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt
    16621 atatgaagca gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct
    16681 tcctgctgtc ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa
    16741 taatttctcc atgatctatc tgtttaacaa tggtggtccc ggaagcgtag gcggaggagc
    16801 tggttctact gatattctta tttcctggat ctataaactt acgactggaa cttctccgca
    16861 gtattctatg gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt
    16921 gctggtattt aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat
    16981 tcgagtgctc gattccaaaa acgactttct cactttttta cttacttttt tcttttaata
    17041 ctgtcagtca ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga
    17101 aatattgcag cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg
    17161 ctattcagtg aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta
    17221 acaatgatcg tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt
    17281 tttatcggcc gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg
    17341 gcagcattga ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg
    17401 ttcttgacaa tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga
    17461 tactttgaca ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat
    17521 ttcaggattt ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca
    17581 ttatgggctt ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca
    17641 cctgaaaatc agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa
    17701 agagtctctc tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc
    17761 ttctttttac aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag
    17821 cagagtatca tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa
    17881 gtgtttgcgg gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta
    17941 gtttccttga tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca
    18001 ttagaacaat ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct
    18061 gaacttgaat tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct
    18121 gaaattttgg taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt
    18181 gattttgaga aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat
    18241 acgatgaatt atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa
    18301 cgatttttag aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt
    18361 ttaagtcttg gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt
    18421 ttcttgtgga tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca
    18481 aatttgatgc tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc
    18541 cactttgact ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta
    18601 tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt
    18661 tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa
    18721 aaattaaact tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt
    18781 ttaaggagga aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc
    18841 tcgctccctg tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac
    18901 actcctcccc aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt
    18961 aacgcttctt tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt
    19021 atccaagcat tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat
    19081 tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc
    19141 catgcgcatt atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact
    19201 tattcttgtg cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa
    19261 gtccatcata tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt
    19321 tgtggctggg gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg
    19381 atcacactaa gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta
    19441 gaagataaat gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat
    19501 catatcacaa gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc
    19561 aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga
    19621 caacaaggcg gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc
    19681 cctggtgtta ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg
    19741 gaaagcttac gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa
    19801 gtccaagatc aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat
    19861 ttacaagctt gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt
    19921 gttcaacctg gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt
    19981 ttaaagacta tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc
    20041 taacttctag gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag
    20101 tttggttctt taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga
    20161 atactatttt ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt
    20221 atttgcagct ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa
    20281 tacgtcttcc agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag
    20341 cacatcttcg tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa
    20401 agtgtctgca gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac
    20461 tagtttggaa ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga
    20521 tcaaaaagaa tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc
    20581 agaaactcta gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt
    20641 tgataaattg atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg
    20701 tacggcaaca gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt
    20761 aaaagacggc aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc
    20821 aactgaacaa gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca
    20881 tgatacacta gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc
    20941 tttgccaatc atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact
    21001 acccaggaat tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt
    21061 acgatcaatg aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt
    21121 atcaattcta tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg
    21181 tatgaaaaag tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt
    21241 gtccaggaaa acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact
    21301 gaattaaatt tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag
    21361 gccacttatg aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa
    21421 ttgcctcctt attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt
    21481 ccattgacct atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa
    21541 caagaagcag tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc
    21601 aagccgactg ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa
    21661 atcattggta caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc
    21721 gcaagcatgt tacagattgg tacagagctt cataaagaag ggccggaaat tttttctaga
    21781 atcataaaag aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt
    21841 aaaggcaaat tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct
    21901 ttttttataa aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga
    21961 cgaacttgtg ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat
    22021 tagcaaaaga gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga
    22081 aaaaaataaa gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga
    22141 gtaattccca cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat
    22201 ttcctttaaa aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt
    22261 tatttgaaac acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag
    22321 gtctagttcc ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc
    22381 cagtcatcta tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa
    22441 gtggaggagc tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg
    22501 gcggtggttc tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc
    22561 gagggacgat gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt
    22621 tcggcttgac agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc
    22681 attccggtgc gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac
    22741 ggttgaagat agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag
    22801 tacttgctgt aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga
    22861 ttccttaccc actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag
    22921 aaacagcagc aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag
    22981 aacttgaacc catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt
    23041 acatcgattc tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag
    23101 aatacgatct attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt
    23161 tgcaaagtga tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag
    23221 gtttagacgt tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt
    23281 tggctaatct gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca
    23341 tgcctctagg aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg
    23401 gctatctatt tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac
    23461 aaggagaaga agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat
    23521 tttaagcgct tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga
    23581 aaaaaagaaa gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa
    23641 tgatcaaaat gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt
    23701 aaatgtcaaa atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa
    23761 aagagaatca gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt
    23821 ctccctttca gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga
    23881 ttcaggaatc cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac
    23941 tagctatatg cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac
    24001 gaatgatcag tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta
    24061 ttatctgcag aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa
    24121 cgtttatttc aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa
    24181 aaagaaaatc gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc
    24241 tgaaaaatat tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt
    24301 gagtgagtga tgtactttcc cctaattgat ttttccctca tgtggacttc acttcctttc
    24361 gtactgcaag gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat
    24421 ttggtagggt tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt
    24481 attcgttttt atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta
    24541 tatttcgggt taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt
    24601 accagcagtg catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt
    24661 cagtgggacg ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt
    24721 ttgccgcaag cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg
    24781 aaaggtactt cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc
    24841 ataggcggtc ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg
    24901 gtcatttgta tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatccatcag
    24961 tcaatctgaa aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct
    25021 tgtatattct gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt
    25081 cggaggtaga aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat
    25141 attgcatcga gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa
    25201 ttgtagatac gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg
    25261 agctgaaagt gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt
    25321 ataattattt tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag
    25381 gaataggtgt tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta
    25441 catttctgat tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga
    25501 caaaccgctt catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag
    25561 gcgtaaaata ttttccaaaa gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc
    25621 aagatccatt ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg
    25681 caatcaatga gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc
    25741 aacaacagca gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc
    25801 ttatttaaga ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt
    25861 gctctccaat aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa
    25921 tgtgatcgaa ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt
    25981 aggacaattc aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc
    26041 tgtcgtccct gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc
    26101 agcgtttctt gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt
    26161 ggcaggagtt gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc
    26221 cgaaaacaaa attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc
    26281 agataaaaca gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat
    26341 tggattttct gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg
    26401 agcgcttgct atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt
    26461 tgtctctgac agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga
    26521 taaaatgaaa aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat
    26581 tgaattattg gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg
    26641 gtcacttatg catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct
    26701 taaatattat tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa
    26761 tgaaaaagga atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag
    26821 tagaagaagt ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc
    26881 ctcaatggca aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa
    26941 aagaaagcta tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcag
    27001 gcgtggatcc cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt
    27061 tatcgattca tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt
    27121 tcttagaaca tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata
    27181 cttatccagc taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag
    27241 ggatgattga atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt
    27301 gactataaaa tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata
    27361 tgatagccaa gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga
    27421 aaatgcttct caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa
    27481 actcccttca tcgagaggtc gtgacatagc ttttgtcacg atctctcggc ttatcctacg
    27541 agtattattc gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg
    27601 aacgctccat cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt
    27661 gctaaatctt ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt
    27721 tttttcaggc gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc
    27781 gtcccgaaat actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt
    27841 cctactgtca gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat
    27901 tggtcccact ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca
    27961 aaataataat cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg
    28021 atcttttgtt ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga
    28081 ccgataaata catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa
    28141 aagctttgga aaatatttcc aaagctttta aaagtaactt tgcttttttg tattttatac
    28201 cagcaagcca gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt
    28261 caatccgtgt tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga
    28321 aatgatcccg atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt
    28381 tgtacgctca gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt
    28441 tgtaaccagt ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc
    28501 gctgatgaag gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa
    28561 tatcccgtgg aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc
    28621 agcaaccgtg atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa
    28681 tgtctgttct ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg
    28741 atttacaatt gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac
    28801 atatttagaa tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat
    28861 agccgataca ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat
    28921 ggaaataaaa acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa
    28981 tttgcccatc ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag
    29041 aatcttgata tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac
    29101 ggaaagaaag aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc
    29161 ttgcccagca aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt
    29221 accaaaagtt gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat
    29281 tagtggtttc aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt
    29341 gacaaataaa ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc
    29401 gttttatttt acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt
    29461 tttagagtga aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta
    29521 aaaagtgtaa cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt
    29581 ttcgttttca acatttataa gtaaactttt tctattggta atatagattt tcggaagggt
    29641 agacaggtgc gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat
    29701 tgaccatcac agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa
    29761 cttgagcgat tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc
    29821 ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa
    29881 ctaatggtga caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta
    29941 agataactgc cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat
    30001 ctggtaattc gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct
    30061 tatctgcata gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt
    30121 cttccataat cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt
    30181 cgcattctgt ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg
    30241 tatcatccac gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa
    30301 cagattcttt accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat
    30361 cccgattata gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat
    30421 tgacatcttt tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg
    30481 gaacattcca tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc
    30541 tttcatgata cagttggttc aagcaagtag cgagctttcc acttcctggg ccaggagctg
    30601 tcactacaac gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg
    30661 agacgatttt ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga
    30721 tgtttcgtct ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag
    30781 tgataacgac actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga
    30841 tatccatatc ataagtaatc ccataatcac cacggatctt attacgttca atatctccag
    30901 catagacgca aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg
    30961 catcttcttc aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact aacttcccac
    31021 caaattctaa atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct
    31081 cttcaatata tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac
    31141 taaagcattt tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa
    31201 aacaaagaaa atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt
    31261 tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat
    31321 atccgttcga tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg
    31381 acgtttactt atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat
    31441 ttcacaacaa gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt
    31501 ctgtcctatc agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc
    31561 agcctgtttt atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta
    31621 ctattgaagt attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga
    31681 gccagataca aatgcaaatt atgagccaga cgaactagag gagacgaacg cggatcagga
    31741 tacaggacaa gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc
    31801 ccaattatta gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga
    31861 gatctaccgt ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa
    31921 tcacgagatc gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc
    31981 ttttatggaa caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta
    32041 ttaccatgat cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc
    32101 taagatcgat gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa
    32161 aatcaatgag ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga
    32221 acaaataaaa gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa
    32281 tcgtaaaaaa atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa
    32341 tgaaaaatta ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga
    32401 agcattgaag caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca
    32461 aaggcaaatc agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt
    32521 cttgatgaat tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga
    32581 gtatgaatga acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg
    32641 aagaaacgaa cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa
    32701 tccctatgct agaggaaaca aaaatacatg aaaaagaaaa agtaactccg acgaatacga
    32761 atactgcaga aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa
    32821 aagagcaaga tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg
    32881 atttacgtat gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg
    32941 atcgttttat attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat
    33001 acttcacgta tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag
    33061 cttatgacac atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg
    33121 aattagtctt ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga
    33181 gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt
    33241 ctttgatccg acactggtca gtggatcgag tattctctgc agggcagatc acgatattca
    33301 atcacaaatt gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga
    33361 cattactaga caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga
    33421 atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa
    33481 agatcaacga aactacttat caagtacgat tagataaagg acagtcttta acgataacaa
    33541 caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca
    33601 ttgatttctt tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg
    33661 ataagttgga ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta
    33721 tccagaataa ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg
    33781 atgaaggagg gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt
    33841 ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga
    33901 ctgctatttt tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga
    33961 gtctttctat tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat
    34021 ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc
    34081 aaaggaatct atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag
    34141 gtatgaccgt ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc
    34201 aaggggtgtc gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc
    34261 tgccagtcgt caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat
    34321 agtttgcttg caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca
    34381 caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta
    34441 caagaaaatg gtcaaaactt ttcggaaaaa gaagtggtct tcaaaaaagg cgatgattta
    34501 ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt
    34561 gaagggcact cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa
    34621 agtgctacta ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat
    34681 ttgtctaaac tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt
    34741 gagttcagtt gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt
    34801 tccgtaattg ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat
    34861 gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc
    34921 aatcagattt tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat
    34981 caatttggtg aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc
    35041 ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat
    35101 gaagtgggta atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga
    35161 agttcgttac gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg
    35221 atgcgctttt tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg
    35281 tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg
    35341 attgctgcat ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc
    35401 attttcctag caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat
    35461 ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa
    35521 acagttcatt taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac
    35581 aaagagaatg aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt
    35641 cctgacaaga agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt
    35701 aaataaaacg tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa
    35761 ataaaacggc tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta
    35821 attaaaggag atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat
    35881 gcagtgatgc cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg
    35941 caaaaagttg gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt
    36001 ggcgcgcagt ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta
    36061 ttgatggttt tctttttaga attacgctat acattattag gagcaagtct tgccggtttt
    36121 atgaaaaaag aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac
    36181 tatgcggtga actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta
    36241 tatgtcaatt ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc
    36301 ggctcggtga ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt
    36361 ttcatgttta tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc
    36421 gtattagggg tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca
    36481 gtcattgctt catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag
    36541 aaactaagag accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag
    36601 gccaatcacc atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc
    36661 atatatcccg cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt
    36721 cagtgactgg atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt
    36781 tttcattact catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct
    36841 agtagcaggc gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc
    36901 gtctgtcttt ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg
    36961 acataagttg ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca
    37021 agtttattcg tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt
    37081 ttaggaatta gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt
    37141 attttatgaa agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt
    37201 ttggctaacc tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg
    37261 gctaagtatg atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg
    37321 ctatcttcct tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc
    37381 aaaaaaagta gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag
    37441 aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt
    37501 tgctccttta ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat
    37561 cagaagttta tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt
    37621 ccaaggaaaa aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg
    37681 agtgatcgtt atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt
    37741 agaagctgta ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca
    37801 tatgtatatc gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag
    37861 ccgttatttc gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc
    37921 gtttattttt gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa
    37981 accactgctt cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag
    38041 ccgtgcttct gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt
    38101 agctataagc agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga
    38161 aaaaacagta ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg
    38221 aatgtcttta ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt
    38281 cggatatttc caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc
    38341 tacgatgctt caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat
    38401 tatcctgaaa gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag
    38461 cttatcaaat ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa
    38521 agaataccga atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc
    38581 caccctgatt atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc
    38641 aatggaatca aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt
    38701 agtattttta ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca
    38761 tcatagcaga agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc
    38821 aactcgtatg attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct
    38881 tacttatgat atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat
    38941 gaaacttgca gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc
    39001 cgacttattc gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat
    39061 cgatatcgaa gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac
    39121 ttcacaacct gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta
    39181 tgacacgatt ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt
    39241 tgcgatgaaa gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag
    39301 tatgccgatg ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct
    39361 tgatgaaata cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt
    39421 agatgatttg aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg
    39481 cagcttatta aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga
    39541 aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa
    39601 taacgaaata caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc
    39661 gattgcggag aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca
    39721 tttcggacca gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc
    39781 gcaataattc cttttattca aaaaacatac caaaaat
//
LOCUS       DAFCLG010000013        38110 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFCLG010000013 DAFCLG010000000
VERSION     DAFCLG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.013"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(451..1761)
                     /locus_tag="LND56_000669"
     CDS             complement(451..1761)
                     /locus_tag="LND56_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6742614.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="LND56_000670"
     CDS             complement(1773..3191)
                     /locus_tag="LND56_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6742615.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="LND56_000671"
     CDS             complement(3431..4999)
                     /locus_tag="LND56_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6742616.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HDYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="LND56_000672"
     CDS             complement(5003..5413)
                     /locus_tag="LND56_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK6742617.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="LND56_000673"
     CDS             complement(5410..6333)
                     /locus_tag="LND56_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBK6742618.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="LND56_000674"
     CDS             complement(6330..7208)
                     /locus_tag="LND56_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK6742619.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="LND56_000675"
     CDS             complement(7195..8532)
                     /locus_tag="LND56_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6742620.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="LND56_000676"
     CDS             complement(8535..9638)
                     /locus_tag="LND56_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6742621.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="LND56_000677"
     CDS             complement(9712..11220)
                     /locus_tag="LND56_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6742622.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="LND56_000678"
     CDS             complement(11186..11878)
                     /locus_tag="LND56_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6742623.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="LND56_000679"
     CDS             complement(11900..12874)
                     /locus_tag="LND56_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6742624.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="LND56_000680"
     CDS             13174..14046
                     /locus_tag="LND56_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6742625.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="LND56_000681"
     CDS             complement(14504..16702)
                     /locus_tag="LND56_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6742626.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="LND56_000682"
     CDS             16934..17926
                     /locus_tag="LND56_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6742627.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="LND56_000683"
     CDS             17986..19407
                     /locus_tag="LND56_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK6742628.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="LND56_000684"
     CDS             19400..20899
                     /locus_tag="LND56_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK6742629.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="LND56_000685"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="LND56_000685"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK6742630.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="LND56_000686"
     CDS             22491..23174
                     /locus_tag="LND56_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBK6742631.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="LND56_000687"
     CDS             23176..24012
                     /locus_tag="LND56_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6742632.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="LND56_000688"
     CDS             24023..25777
                     /locus_tag="LND56_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742633.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="LND56_000689"
     CDS             complement(26053..26394)
                     /locus_tag="LND56_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6742634.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="LND56_000690"
     CDS             complement(26462..28633)
                     /locus_tag="LND56_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6742635.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="LND56_000691"
     CDS             28802..29701
                     /locus_tag="LND56_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6742636.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="LND56_000692"
     CDS             complement(29772..30629)
                     /locus_tag="LND56_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6742637.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="LND56_000693"
     CDS             complement(30614..33316)
                     /locus_tag="LND56_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK6742638.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="LND56_000694"
     CDS             complement(33329..34618)
                     /locus_tag="LND56_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBK6742639.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="LND56_000695"
     CDS             34768..35814
                     /locus_tag="LND56_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6742640.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="LND56_000696"
     CDS             35816..37969
                     /locus_tag="LND56_000696"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBK6742641.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11245 a         7841 c         6770 g        12254 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatc gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca
//
LOCUS       DAFCLG010000014        36568 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFCLG010000014 DAFCLG010000000
VERSION     DAFCLG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.014"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LND56_000697"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LND56_000697"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK6742642.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LND56_000698"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LND56_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK6742643.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LND56_000699"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LND56_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK6742644.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LND56_000700"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LND56_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK6742645.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LND56_000701"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LND56_000701"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK6742646.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LND56_000702"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LND56_000702"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK6742647.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="LND56_000703"
     CDS             complement(8053..8523)
                     /locus_tag="LND56_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBK6742648.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="LND56_000704"
     CDS             complement(8569..8994)
                     /locus_tag="LND56_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBK6742649.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="LND56_000705"
     CDS             complement(9061..9330)
                     /locus_tag="LND56_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBK6742650.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="LND56_000706"
     CDS             complement(9341..10936)
                     /locus_tag="LND56_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6742651.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LND56_000707"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LND56_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK6742652.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="LND56_000708"
     CDS             complement(14489..15049)
                     /locus_tag="LND56_000708"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK6742653.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="LND56_000709"
     CDS             15402..16376
                     /locus_tag="LND56_000709"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6742654.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="LND56_000710"
     CDS             16546..17946
                     /locus_tag="LND56_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBK6742655.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="LND56_000711"
     CDS             complement(18024..18746)
                     /locus_tag="LND56_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6742656.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="LND56_000712"
     CDS             complement(18825..19331)
                     /locus_tag="LND56_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6742657.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="LND56_000713"
     CDS             19454..21253
                     /locus_tag="LND56_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBK6742658.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="LND56_000714"
     CDS             complement(21321..22895)
                     /locus_tag="LND56_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6742659.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="LND56_000715"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="LND56_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK6742660.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="LND56_000716"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="LND56_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK6742661.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="LND56_000717"
     CDS             complement(24672..25310)
                     /locus_tag="LND56_000717"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBK6742662.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="LND56_000718"
     CDS             complement(25311..25961)
                     /locus_tag="LND56_000718"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK6742663.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="LND56_000719"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="LND56_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBK6742664.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="LND56_000720"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="LND56_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBK6742665.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="LND56_000721"
     CDS             complement(29104..29844)
                     /locus_tag="LND56_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBK6742666.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="LND56_000722"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="LND56_000722"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK6742667.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="LND56_000723"
     CDS             complement(31215..32153)
                     /locus_tag="LND56_000723"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK6742668.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="LND56_000724"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="LND56_000724"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6742669.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="LND56_000725"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="LND56_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK6742670.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="LND56_000726"
     CDS             complement(35313..35618)
                     /locus_tag="LND56_000726"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6742671.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="LND56_000727"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="LND56_000727"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK6742672.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DAFCLG010000015        34660 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFCLG010000015 DAFCLG010000000
VERSION     DAFCLG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.015"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(313..2205)
                     /locus_tag="LND56_000728"
     CDS             complement(313..2205)
                     /locus_tag="LND56_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742673.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="LND56_000729"
     CDS             complement(2202..3929)
                     /locus_tag="LND56_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742674.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="LND56_000730"
     CDS             complement(3945..4595)
                     /locus_tag="LND56_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6742675.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="LND56_000731"
     CDS             4799..5095
                     /locus_tag="LND56_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6742676.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="LND56_000732"
     CDS             5287..5907
                     /locus_tag="LND56_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK6742677.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="LND56_000733"
     CDS             complement(5999..7165)
                     /locus_tag="LND56_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6742678.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="LND56_000734"
     CDS             complement(7177..7530)
                     /locus_tag="LND56_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6742679.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="LND56_000735"
     CDS             7658..8680
                     /locus_tag="LND56_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBK6742680.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="LND56_000736"
     CDS             8699..8860
                     /locus_tag="LND56_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742681.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="LND56_000737"
     CDS             complement(9119..9553)
                     /locus_tag="LND56_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742682.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="LND56_000738"
     CDS             complement(9663..11522)
                     /locus_tag="LND56_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6742683.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="LND56_000739"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="LND56_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="LND56_000740"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="LND56_000740"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK6742684.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="LND56_000741"
     CDS             complement(14546..15244)
                     /locus_tag="LND56_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6742685.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="LND56_000742"
     CDS             complement(15201..16862)
                     /locus_tag="LND56_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBK6742686.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="LND56_000743"
     CDS             17080..18480
                     /locus_tag="LND56_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBK6742687.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="LND56_000744"
     CDS             complement(18732..19811)
                     /locus_tag="LND56_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6742688.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="LND56_000745"
     CDS             complement(19897..20856)
                     /locus_tag="LND56_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6742689.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22379)
                     /locus_tag="LND56_000746"
     CDS             complement(20868..22379)
                     /locus_tag="LND56_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6742690.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="LND56_000747"
     CDS             complement(22401..22964)
                     /locus_tag="LND56_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK6742691.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="LND56_000748"
     CDS             complement(22970..23833)
                     /locus_tag="LND56_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6742692.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="LND56_000749"
     CDS             complement(23847..24743)
                     /locus_tag="LND56_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6742693.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26207)
                     /locus_tag="LND56_000750"
     CDS             complement(24918..26207)
                     /locus_tag="LND56_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6742694.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="LND56_000751"
     CDS             complement(26179..27696)
                     /locus_tag="LND56_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6742695.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="LND56_000752"
     CDS             27894..28784
                     /locus_tag="LND56_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBK6742696.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="LND56_000753"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="LND56_000753"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK6742697.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="LND56_000754"
     CDS             complement(29693..31165)
                     /locus_tag="LND56_000754"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK6742698.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="LND56_000755"
     CDS             complement(31802..32164)
                     /locus_tag="LND56_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK6742699.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="LND56_000756"
     CDS             32387..33139
                     /locus_tag="LND56_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6742700.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="LND56_000757"
     CDS             complement(33279..34202)
                     /locus_tag="LND56_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6742701.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="LND56_000758"
     CDS             complement(34217..34489)
                     /locus_tag="LND56_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742702.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10229 a         7212 c         5980 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaagt
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DAFCLG010000016        33473 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFCLG010000016 DAFCLG010000000
VERSION     DAFCLG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.016"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..1100
                     /locus_tag="LND56_000759"
     CDS             237..1100
                     /locus_tag="LND56_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6742703.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1229..2851)
                     /locus_tag="LND56_000760"
     CDS             complement(1229..2851)
                     /locus_tag="LND56_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6742704.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3100..3621
                     /locus_tag="LND56_000761"
     CDS             3100..3621
                     /locus_tag="LND56_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6742705.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3807..5537)
                     /gene="spxB"
                     /locus_tag="LND56_000762"
     CDS             complement(3807..5537)
                     /gene="spxB"
                     /locus_tag="LND56_000762"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBK6742706.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5702..6520
                     /locus_tag="LND56_000763"
     CDS             5702..6520
                     /locus_tag="LND56_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBK6742707.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6623..6820)
                     /locus_tag="LND56_000764"
     CDS             complement(6623..6820)
                     /locus_tag="LND56_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742708.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6989..7567
                     /locus_tag="LND56_000765"
     CDS             6989..7567
                     /locus_tag="LND56_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6742709.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7662..7799
                     /locus_tag="LND56_000766"
     CDS             7662..7799
                     /locus_tag="LND56_000766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742710.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8612..9115)
                     /locus_tag="LND56_000767"
     CDS             complement(8612..9115)
                     /locus_tag="LND56_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6742711.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9474..10499
                     /locus_tag="LND56_000768"
     CDS             9474..10499
                     /locus_tag="LND56_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6742712.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10822..11700
                     /locus_tag="LND56_000769"
     CDS             10822..11700
                     /locus_tag="LND56_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBK6742713.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12176..12292
                     /locus_tag="LND56_000770"
     CDS             12176..12292
                     /locus_tag="LND56_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK6742714.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13220..13558
                     /locus_tag="LND56_000771"
     CDS             13220..13558
                     /locus_tag="LND56_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742715.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13652..13882
                     /locus_tag="LND56_000772"
     CDS             13652..13882
                     /locus_tag="LND56_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742716.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14282..14653
                     /locus_tag="LND56_000773"
     CDS             14282..14653
                     /locus_tag="LND56_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK6742717.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14927..15619)
                     /locus_tag="LND56_000774"
     CDS             complement(14927..15619)
                     /locus_tag="LND56_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6742718.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15622..16407)
                     /locus_tag="LND56_000775"
     CDS             complement(15622..16407)
                     /locus_tag="LND56_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6742719.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16644..18044
                     /gene="lacG"
                     /locus_tag="LND56_000776"
     CDS             16644..18044
                     /gene="lacG"
                     /locus_tag="LND56_000776"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK6742720.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18069..18386
                     /locus_tag="LND56_000777"
     CDS             18069..18386
                     /locus_tag="LND56_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6742721.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18399..20072
                     /locus_tag="LND56_000778"
     CDS             18399..20072
                     /locus_tag="LND56_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBK6742722.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20086..21717
                     /locus_tag="LND56_000779"
     CDS             20086..21717
                     /locus_tag="LND56_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBK6742723.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22589..23779
                     /locus_tag="LND56_000780"
     CDS             22589..23779
                     /locus_tag="LND56_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBK6742724.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23913..26102
                     /locus_tag="LND56_000781"
     CDS             23913..26102
                     /locus_tag="LND56_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6742725.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26099..27892
                     /locus_tag="LND56_000782"
     CDS             26099..27892
                     /locus_tag="LND56_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6742726.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28092..28406
                     /locus_tag="LND56_000783"
     CDS             28092..28406
                     /locus_tag="LND56_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6742727.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28427..28804
                     /locus_tag="LND56_000784"
     CDS             28427..28804
                     /locus_tag="LND56_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6742728.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28814..29587
                     /locus_tag="LND56_000785"
     CDS             28814..29587
                     /locus_tag="LND56_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742729.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29609..31012
                     /locus_tag="LND56_000786"
     CDS             29609..31012
                     /locus_tag="LND56_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6742730.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31194..32378
                     /locus_tag="LND56_000787"
     CDS             31194..32378
                     /locus_tag="LND56_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6742731.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32375..32665
                     /locus_tag="LND56_000788"
     CDS             32375..32665
                     /locus_tag="LND56_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742732.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32662..32883
                     /locus_tag="LND56_000789"
     CDS             32662..32883
                     /locus_tag="LND56_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742733.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32925..>33473
                     /locus_tag="LND56_000790"
     CDS             32925..>33473
                     /locus_tag="LND56_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742734.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLY"
BASE COUNT        10867 a         5512 c         6596 g        10498 t
ORIGIN      
        1 acctctgatt cacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa
       61 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
      121 caacctctta ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa
      181 gagatttgct ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt
      241 atcaaattgt aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag
      301 ttgatttttt atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa
      361 agacattcaa ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat
      421 cacaaatcaa tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc
      481 ctgttttata tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag
      541 cggttgaatt gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag
      601 ctgcaagtat tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta
      661 aaagcatcga agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg
      721 taacagtaga cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta
      781 ctcttggcag tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga
      841 cgaatgttga caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg
      901 taaaaacaat cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag
      961 agagcgcgga gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc
     1021 tttatccctt aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt
     1081 cgatgggaat cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt
     1141 gtaacaaaaa cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt
     1201 ttttttattt tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa
     1261 tatctgcatt ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat
     1321 ccgaaacggc aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa
     1381 ataagattga gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt
     1441 ctaaatggtt cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg
     1501 aaagcatgca acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt
     1561 caccagaaaa caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt
     1621 tacttgcata ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg
     1681 tatcctttgg taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg
     1741 gcgtaatttc acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga
     1801 cgaaagcgac cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc
     1861 catcaatcgt tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag
     1921 gtttgaatcc aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt
     1981 tttcaagcat ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc
     2041 gggcaataaa ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt
     2101 gaagtttagc cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt
     2161 tgattttacc aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt
     2221 catgggatac aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga
     2281 tcgattggat atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa
     2341 atagtgtttg tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct
     2401 gatggtgcaa ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag
     2461 cttcccatcc attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt
     2521 cgtcagtgaa atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac
     2581 gcttgtgtcc catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct
     2641 gtttcaatac ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca
     2701 gatcccctga taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc
     2761 catagcagtt tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa
     2821 agtgcaaact cacatcattt acagtaatca atttttttcc tcacttttat cattattagt
     2881 tttgacaagt cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat
     2941 tctttgtgtt tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag
     3001 ctactatagg atttattagt ttgcaacagc ataatttttg acgttattta taagagggag
     3061 tactataaaa ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc
     3121 gactggggct cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa
     3181 gaccctgtcg gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa
     3241 gggatatttg aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag
     3301 atgcccttaa taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt
     3361 ggtgcaggag tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt
     3421 ttagcgttat ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg
     3481 atcatcgtca atattttagg aattttatta ggaattttct tattgtttaa tccaatttct
     3541 tctgcgttga cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat
     3601 atcgtctatg catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca
     3661 gttttactgt tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat
     3721 ttttaggtgt cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc
     3781 ttaatactcg aagcatagcg cagttgtcac aacctctttt catataattc attcaatact
     3841 ggcaaatcat ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt
     3901 cttggatcta aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg
     3961 attactacag gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat
     4021 tctgtgactg taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct
     4081 tctaaaaata caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca
     4141 aaagaatcat tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg
     4201 atatcttgca tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct
     4261 gggtagctta gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat
     4321 ccagatgtcg tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc
     4381 gtgttaccga catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta
     4441 tagacagcct ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt
     4501 acccattttt ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc
     4561 cctaactcga caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt
     4621 tcatggcgtc gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga
     4681 ttaaaaaatg cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct
     4741 gccaatgctt catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca
     4801 taatcatcag gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt
     4861 tctgagaatt ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact
     4921 ggtttttttg ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat
     4981 atcacacttg ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa
     5041 ccaagatcaa cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg
     5101 gcttcatcta ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta
     5161 acatctgcga aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta
     5221 gaagcaactt gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa
     5281 ccattgatca aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc
     5341 gttaattttg catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg
     5401 tagttgattt cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg
     5461 ggaatgccgt aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct
     5521 gcattgattt ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt
     5581 atctagagta tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt
     5641 agtcatgttt ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg
     5701 catggttgaa tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt
     5761 tacttatttt atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa
     5821 agaagtgcta aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg
     5881 gtctttgtta gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt
     5941 tgtaagtatt gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc
     6001 ccatatgcat tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac
     6061 gatcttgctt gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt
     6121 agcattcggt gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc
     6181 ggtggcgcta ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt
     6241 gcgacaagaa ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt
     6301 tgaaccaatt gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc
     6361 ttatgcactt gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc
     6421 tgaagcacag cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg
     6481 atttatcatt atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat
     6541 atatcaaata gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata
     6601 atgaagtgaa tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc
     6661 agcgaacaag ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa
     6721 gcccgcttag gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa
     6781 gaaccatgaa gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc
     6841 attctttact atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact
     6901 gatttttact tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta
     6961 aaatagagcg gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga
     7021 tgctgtaatc gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga
     7081 cacgtgggag gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat
     7141 cattttagcg atttattgga acaatcatca tcatttattt caagttgtac ataagataaa
     7201 tggacgggtc ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc
     7261 aacttcatgg atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt
     7321 taccttgacc gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa
     7381 aggccacgag aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat
     7441 tggtttgaat gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat
     7501 cgcaaacatc gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata
     7561 tcggtaaaat gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga
     7621 tcaaatatcc gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc
     7681 atgtaggact gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga
     7741 cttggacagt agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa
     7801 catctttatg taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat
     7861 tctgttgtaa acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa
     7921 atagatagtt agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt
     7981 caaagaaggg cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt
     8041 aacgtgtttt caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc
     8101 attttacgct tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca
     8161 gatttctcta ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta
     8221 ggatcaggca gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac
     8281 catagaaaat ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct
     8341 gagtagggta taaattaaga cggaattcct ttctagattg agatattgac taggaagaat
     8401 gttaagattt taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata
     8461 gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa
     8521 aatagatttt tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg
     8581 tgtctattac ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa
     8641 ataaaaaagc tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt
     8701 ctaacttatt tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc
     8761 ctcctgttgt attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt
     8821 gaatcaattc tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa
     8881 gtctcttgaa attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag
     8941 tggtctcacg taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga
     9001 cattgctgta agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca
     9061 accataatag aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca
     9121 tctcctccat ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa
     9181 gtttaaggac caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg
     9241 gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc
     9301 aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata
     9361 ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt
     9421 ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat agattgttca
     9481 agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc
     9541 tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt
     9601 tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc
     9661 tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat
     9721 cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat
     9781 tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg atgctttctg
     9841 ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt
     9901 ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc
     9961 ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga
    10021 tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct
    10081 tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa
    10141 acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat
    10201 gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca
    10261 cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt
    10321 ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt
    10381 tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt
    10441 gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga
    10501 aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca
    10561 aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga
    10621 actttcaaaa aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa
    10681 taaactttcc tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat
    10741 agaaagttta agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc
    10801 ggataatcag tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag
    10861 aaaataagca atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata
    10921 gagcaaatga ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat
    10981 gaactaatgg atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa
    11041 gaggaacctt ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa
    11101 atttatcaac cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact
    11161 agatttattc atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg
    11221 atttggcatt acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca
    11281 tattttccga aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat
    11341 attccttccg atgcagattt atctcagaat aagataagag atagttacga aagtgccatt
    11401 cgtttttttg aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg
    11461 ttactatcac ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca
    11521 gccgattatc gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt
    11581 ggacgagtag atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca
    11641 aaagagcaat tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa
    11701 gtttagtact tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct
    11761 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
    11821 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
    11881 cttctgtccc agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa
    11941 ctagctataa caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa
    12001 acgtaataat gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt
    12061 tttaacaagt tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt
    12121 ttcgatttgt gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga
    12181 aaaaacagat atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa
    12241 acgtgcgctg aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg
    12301 aaacttaatt aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt
    12361 gtgtaaggtt tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt
    12421 agaatctttc ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga
    12481 accacaaagc acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca
    12541 acctcttatt ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt
    12601 gaaacatttt tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt
    12661 tcatgaaata tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat
    12721 aagcagttac gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca
    12781 aaaaaatcag tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac
    12841 gccgaaaaaa tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga
    12901 ataataagtt ttggttttca gataatagtc gataagttgt gtattggaat tttagataca
    12961 gtaataaagt atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa
    13021 attaatggtt aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt
    13081 gtgatactag actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa
    13141 atgaaaaaga agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt
    13201 tagccaagta acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt
    13261 tcgtgctaca gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc
    13321 tagtgagcca attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt
    13381 tatggtggta aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat
    13441 tgaaaaggtg agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat
    13501 cattttaacg gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac
    13561 actagtgaaa tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa
    13621 atcgatcatc aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat
    13681 ttattacgtg aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag
    13741 tcgctactca attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa
    13801 aactgttttt tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga
    13861 ttatgttagt atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg
    13921 ttattgtttt ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt
    13981 atccagagta ttttatttta acttttgtat atttagtaag tagctacaca cttctatata
    14041 aattcagaag ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat
    14101 aatatttttg atttatatag gaaattaaaa atattataga atcaacactc agagaacggg
    14161 ttggttttta atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga
    14221 agttggttac cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat
    14281 aatgagagat tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa
    14341 tgaattatct gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga
    14401 ctatattgat atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat
    14461 aaaagctttt atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct
    14521 agtaggaaaa ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc
    14581 tttgtctatg ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt
    14641 ggaaatcttt taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga
    14701 tgatttttaa gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag
    14761 atagagatga acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat
    14821 ttcactaagg atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag
    14881 gaggtgtgca tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg
    14941 atgtggcaga caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat
    15001 ttagctgtgc ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg
    15061 ttccgatccc cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac
    15121 aatcagccgg agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt
    15181 tcccagggaa agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat
    15241 cgaaaatatc aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg
    15301 gaatctgttg gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct
    15361 cgttttcatt ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg
    15421 cgatctcaga ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc
    15481 gaccatgaat attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta
    15541 tcatatgcag ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat
    15601 caaatataat ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat
    15661 ttgcagatcg acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt
    15721 aatgagcaga tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt
    15781 tttattatca acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc
    15841 tccttcttcg gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa
    15901 tgcttttgta aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc
    15961 ggtatttttc cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag
    16021 aatatcaaaa actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt
    16081 cgccaatagt tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa
    16141 ggaggctgct gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt
    16201 gtgagaaagt tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag
    16261 cagcttgccc gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc
    16321 taattgattg atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat
    16381 agtaagcaat ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca
    16441 tataaacgga caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat
    16501 gtttgttaca atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt
    16561 tgttgacaaa tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat
    16621 tatagatttg aggagagatg tacatgctta aattaccaga agattttatt tttggcggag
    16681 ccacagcagc ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt
    16741 gggatgactt tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc
    16801 atcaatatgc aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat
    16861 ccattgcttg gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg
    16921 ctttttatca tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt
    16981 tgcatcattt tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa
    17041 caatagaagc atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg
    17101 tgtggagtac ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt
    17161 tcccaccagg aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg
    17221 ttgctcatgc ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg
    17281 tagttcactc attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg
    17341 cctttttaga cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt
    17401 attcaactga aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc
    17461 attttcctga agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag
    17521 ggatcaatca ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc
    17581 ataacggtac gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta
    17641 tttataaaga aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat
    17701 atgacttgtt gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg
    17761 agaacgggat ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa
    17821 tcgactattt gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg
    17881 tgaaggggta ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca
    17941 aaaggtatgg cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag
    18001 cttactggta caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg
    18061 aagaaaagat gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag
    18121 gcgacgctcg cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac
    18181 atgttgattc ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa
    18241 caaaaatttt agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc
    18301 atgggcagga tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg
    18361 tactgtacag acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa
    18421 tcgaaaaagg gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc
    18481 gtgacggatt tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg
    18541 ctgcggttcc tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga
    18601 aagtctataa ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct
    18661 gcttggctga ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg
    18721 tgatgctggc ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat
    18781 tttcaacgga atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa
    18841 cagttaattt atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag
    18901 ttcctggaac gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg
    18961 cggccgtttt aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa
    19021 tcattacggt gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta
    19081 tctggggagc gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc
    19141 cggcaattgc ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg
    19201 gtgaacaagc ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag
    19261 gaactggggc aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt
    19321 tgaaggcagt tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc
    19381 tatttgcggc acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg
    19441 ttgtaaacgt ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt
    19501 acgttttgcc ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta
    19561 tcttagcttt tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat
    19621 tttgcaaagc atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg
    19681 caaccgagga aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag
    19741 ttatcagcga tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa
    19801 gtgcgatgtt agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg
    19861 caatggcggg tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct
    19921 tagcaccgca agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa
    19981 tcaagctggt tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag
    20041 ctgttcagtt tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt
    20101 tggttgcgta aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa
    20161 actgttgaag catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat
    20221 atggaaaaat ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt
    20281 ttaaaaaata atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt
    20341 aatggagaaa gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa
    20401 cgagcaaaaa atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt
    20461 gatcctggga aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat
    20521 acggcattgt acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat
    20581 cctgatatgg ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag
    20641 tcttggggag aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc
    20701 caagctgttc gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt
    20761 ggcgatacag gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt
    20821 gatttaattg gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc
    20881 aatatgaatg cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat
    20941 gggcatacaa cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct
    21001 gcggggggat atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat
    21061 cggattcagg cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg
    21121 tacaacggaa atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc
    21181 actatgggaa acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca
    21241 agcttgcgtg cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc
    21301 gaaagcgatg gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc
    21361 gctaagggta cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt
    21421 tggcaggatc aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga
    21481 acttacacat tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat
    21541 atcaaaaatt atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca
    21601 aaaattacaa tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc
    21661 aaggcttcag gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact
    21721 gcggatcgtt tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc
    21781 aggaatcgaa cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt
    21841 ttaccaaaac gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt
    21901 caacgatttc gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt
    21961 tgtttaagaa agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga
    22021 tagaaaaaag ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg
    22081 aaacaaatta acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa
    22141 atcaagcatt ttagctattg aagaatgttg atttataggg attccccctc catattatgt
    22201 tggctcttta ttcccactct gttttcaacg taaagtgtat aaaacagaat gcgatactat
    22261 caagggagtt aaaacaatat acagaacagt aaaacactat aaataaaaat aaaagtgcaa
    22321 taaaagtgca atttttgcac ctaatcatac tctgtttata tatgaaaact agattctgaa
    22381 tccaatattt ggttaacgag aggcgttcga atttgaacgt cttttatata ttactttcaa
    22441 ataaatatga ggtagaatac tatctatttt ctttttaacc caattgttat aatggagagt
    22501 gtaaaatatt ttgtgtaaat agaaaaaagg aagtcccttc tgtagaatag agttaccaca
    22561 acacattcac agaagagagg acttccctat gaacgatttt actacagaaa ttctaaagac
    22621 tctagcgaac aaaggcgatt tgaatgaatt attccgtgtc catttggaga aagcagtcaa
    22681 tacgcttctc aaaacggagt taacggcttt cctagattac gaaaagtatg atcgcattgg
    22741 ttttaacacg ggtaattctc gtaacggctc ctatgaccgt acggtcaaga ccgagtatgg
    22801 ggaacttcat ctccagattc cgcgcgaccg caatggtgag ttcaagcaac agactgttcc
    22861 tgcttataga cggacgaatg acacgttaga ggagaccgtc attcacctct tccgaaaagg
    22921 tattaccatg tcggaaatcg cagacttgat tgagaaaatg tatgggcatt actacacgcc
    22981 ccaaaccatg tccaatataa caaaatcatt tacagaagag gtaacggcgt ttaatgggcg
    23041 ggagcttcat gaccgttatg ctgctattta tatggacgca acgtatattc cgttaaagcg
    23101 gaaaaccgtc gccaaggaag ctattcatat cgcagttggc attcgcccgg acggatcaaa
    23161 ggaagtattg agctatgcga ttgcaccgac tgaatccatc acgatttggg aggaaatttt
    23221 attggacctt caagagcgcg gtttgaaaaa tgtcctcctg ttcatcacgg atggcttaaa
    23281 ggggatggta ggagcgatta gtcggttcta tcccaaagct cgttttcaac attgttgtgt
    23341 acacgtttcc cgtaatatca gtcataaagt gcgtgtcgat gatcgtaagg aagtctgtga
    23401 tgattttaaa atggtgtatc aagcctcatc taaagaggtg gcgttggaag cacgtggtac
    23461 ttttgcggaa aaatggaaaa ccagctatcc aaaagtggtt gaatcgattc tttcgaacga
    23521 tcacttgctc actttctatg atttcccctt ggccatacgc aagagtattt attctacgaa
    23581 cttgattgaa tcctttaata aacaaatcaa gaaatacagc caccgcaagg aacagttcca
    23641 aaatgaagag tcgatggaac gtttcttagt ctcgtctttt gatacttaca accaaaaatt
    23701 cctaggtcgc agtcataaag gtttccaaca ggccgaaggc gagctcgaac aaatgctaag
    23761 ccaactgatt gagaattaga aacaatctgc agtagggaag aatcatttac acaaaattat
    23821 tgacgctccc ttataatgag aacttttact aacataattt atgataaact tactatataa
    23881 tcatattaat taaatagtta ggagatctgc ttatgtttat atctactctt caaattcgaa
    23941 attttagaaa ttatgaatcc gaaatctttc gattcgataa tgagacaaat actattatag
    24001 gtgagaatga ttcagggaaa accaatgctc ttactgcatt gagaatctta ttagatgata
    24061 gttattacta tagtagtaaa acactaaaag aatcagattt ttttcatgga attcaaaatg
    24121 ggtggcaagg gcattggatt attatctctg cgacatttga aggaatcagc gaagaagaat
    24181 ttgacaatga gatttgtgct tccatttctt taaattctga aagtcaaaca atcttagaag
    24241 aattgatttc aaatgcagat aaaggtgtag gtagtatttc tctatttatt agacctaaca
    24301 aagctattag aaagcaatta tttgatattt ccactgaatt agactctcat caatttaacg
    24361 aatttagaaa ttctattcgt ctatccgact atgaatttta ctacacatct aaatcaaatc
    24421 taaatttctg tatcaatgaa aattatgata aattggtagg aagactcaat gacaatatag
    24481 ctagtaatcc tgaagaagac gatgaagctc tgttgggctc aagaattgac atgtcagata
    24541 ttttcaagca tatttcggtt gtttatgttg acgctcttcg agatgttctt agagaaatga
    24601 aaaacaatcg aaacccagtc aaaagaatca tggaaacaat tgaatcaaaa atatcatcag
    24661 ataatgttga gtcattaaaa acaattatac agcaattaaa tgagactatt accagtgttc
    24721 ctgaaattag gaaaattggc gaaaatatta atcggcaact aaatagcata attggttcag
    24781 tgtattctcc taatcttctt ctttcctcaa ctatgtcaga tgatatgggt tctttagcaa
    24841 aatttctttc aatgaagcca gaacaaaata tcgacttaga cttactaggg ttaggacatc
    24901 taaatatgat ttatttagct ttgaaaatag tcgaatttga agcgtgtcgt tcaagagaac
    24961 tacttaatat tatgttgatt gaagaaccag aagctcatat tcatcatcat attcaaaaaa
    25021 ctctgttcga aggattaaat cttcaaaaaa actatactca gattctcatg accacacatt
    25081 ctgtacattt agctgaagtg tctgaaatat ctcggatgaa tgtattaaaa tcgtttaatg
    25141 gcaaatctat cgttatgaat ccagcaaata atctgaactc atttgctaaa gaaaagttaa
    25201 aaaagaacaa tctaaatcta ataaacgcag tagagagata tttagatgtt aaaagaaatg
    25261 gattgctttt ttctaaaggt gttatcttag ttgaaggaga tgctgaagaa atacttattc
    25321 ctcaaatagt aaaaaaatta tttggtataa acttagacga acttggaata ggtttgatta
    25381 atgttggtag tacttcgttt gaatatatcg ctagcttgtt tgacgatgat agaatacaga
    25441 gaaagtgtgc tattgtaact gatttagatg aacaggcaat cccatcagaa catcgattgt
    25501 ataaaagcac tgcacaagag aaaggtcaag caagaaaaga aaaattatcc tcccttttta
    25561 atgaaaacaa ttgggttaat tgtttttatg ctaatacaac atttgaaatt gagtttttag
    25621 atgtgaataa agaaaatatt ctaacttatg tcagtccaat tatagatgac gcattcaaag
    25681 ataaaaaaac aataactgct tataaagaag aaatacaaag tgaacaatat ttagaaagaa
    25741 atttagcgat gttaaaacta gccaatcatg ttggtaaggg gtggtttgca attgagttag
    25801 gtgaaaaaat tgacttttta atcaaaattc ctaagtatat tcttaatgct attgcttttg
    25861 catgtcagga aatagtagat ctctcaattt ataaaaaaat tctactccat acaatcgact
    25921 tgtacgagga aactacagaa ttgtcagctt tatcagctag tttaaaaaca agtgatacag
    25981 ctgatttgaa tatagcaata atttctttat tagagtactt tggtaaagat gaggatgttg
    26041 tgtatctaat caatcagata gaaaattaca caaaaatact ttatgctgag gtttcaaaat
    26101 gacaaatatc ttcgaaaaaa tcgtaaatga tttaaattta gaacaaaaag atgttgtgga
    26161 aagtgaagat aacctttatg taaatgcatg tcctgggagt gggaaaacaa gggtacttac
    26221 aaggaaaatt gcttaccaag caattaaaca cgatggatca ctaaaaagaa taattgctat
    26281 cacatacact aatagagctg ctgaagaaat taaggaacgc ctaagtttac ttagtataga
    26341 tgatgatatt aatatttggg tggggacaat acatcagttt tgtcttgaat ttattattta
    26401 tccttttaaa atgaatctct caagagtatc aaaaggattt acaattattg atgattatac
    26461 tcaaagagct tacgtcagcc aaattaataa tatgctgaat ttaaatctca aaacgtatga
    26521 actgaacaaa attgatttgt cactttctca tgattttaaa attaatgaag ttcagtatac
    26581 tcaagtttct gaaatgtatc acactatgtt ggaaaaaaat aaagaaattg attttgatat
    26641 gattttacta gtagcgtata aaattttaac tgtaaacagt aacgttgcac gtaatattgc
    26701 tagtaatata agggcgattt atgtggatga atttcaagat actagagaat tacaatacaa
    26761 tgtaatagct aaattacttc aaaacaaccc tcaaattcag tcaatgtttg ttggagatat
    26821 tgaccaagca atttatggat cattagatgg tatagctaaa tcagttgaag aactagaaat
    26881 gctaactagt cacacttttc aatcaaagac gctacatggc tgttacaggt ctaatcagag
    26941 attagtagac ttctactcta attttcagag ttgtccttac gagattgaaa gcagagggaa
    27001 taataaaaac gaccgtggtt ttataagtta tgattgcaaa gttactaagg aggatttacc
    27061 caacataata aaaaatataa taaaggaaaa gattgaaagc ggcattcatg aaaaagatat
    27121 ttgtgtactt gctcctcaac actatcctct gttttcactg ggtgagtatt taaagaaaga
    27181 attaccgtat tgcaattttg atgctataaa tatatcacca attaaggtaa ataatcatag
    27241 tttatttttc aagttagcaa ttttgtactt tactgaatca ggtgaaaagg ttcgaagaag
    27301 aaagctaatt gcaaacgata ttttaattat attaaaaaat gaatttggaa tagaagttaa
    27361 agattatttt attgacttag atttattaca gctaataaac agtgtgaaaa gaatgtatag
    27421 tggagacgac aacggtgttg atttatacaa atttgttacc aaacatatat ttgttaatct
    27481 taatattgac gagaaaaatt acccgtctat ttttaagcac tatctatttt ttattaacga
    27541 agtagaagat cggataaaaa ataattcatt atctagtagt gtatctagtt ttaagaagat
    27601 gtttaaatct cgagatggta tcacacttac tacagcacat aaagttaaag gaaatgagta
    27661 tgatactgtt atcgctatta atcttctaaa cgggaaaatt cctcattgga atgaaatttt
    27721 taatgtttct gataaaggaa ttagttcagc gagaaaacta ctatacgtaa tttgttcaag
    27781 agcaaaaaat aatttatttc ttttctcaga ggtgggttat tttacgagta aaggagactt
    27841 gctttatccc actcaattat tagataaaat agaatttgat tataatattt aaaataccta
    27901 ggtagtcgtt gttttaaata tagtaggaat cggacgctca gtacggagcg tctatttttt
    27961 tacccaattt tgaaaggaag tgaacttatg aaaataaaaa atcaagaatc aaaaggtcgc
    28021 tctcccccct ttaaaaccat cacacaaata agattcattc aaacataaaa acagaaagga
    28081 taggtaaaaa tatggaactt aaatatgtca ttcccaatat ggaaaaaaca tttggacaat
    28141 tagaatatgc tggtgaaggg aacattgaac aacgtcgaat aaatggcaga ccaaccattc
    28201 tctctcgtag ttacaattta tactcaacga ttcaacgagc agatgatatt gttgtcattc
    28261 ttccctccga agctggcgag aaacattttg aatccgacga aaaagtcaaa ctcatcaacc
    28321 cacatattac cgcagaaggt tataaaattg gcaatcgagg atttaccaat tacattatgc
    28381 acgcagacga tatggtgaaa gcataagaaa ggaaaggtga aaaaatatga gattagcaga
    28441 aggtattgtg attgaaaaag accagacatt tggcacattg aaattttccg ccttgcgtcg
    28501 tgaggttcgt atccagaatg aagatggtac ggtttccgaa gaaattaaag aacgtaccta
    28561 tgacttaaaa tcaaaaggac aaggacgcat gatacaagtc agtatccccg cttctgttcc
    28621 tttgaaagaa tttgactaca acgcagaagt ggaattaatc aatcctgtgg cagataccgt
    28681 agcgacggct acttttcgag gggcagacgt tgattggtac gtgaaagcag acgatcttgt
    28741 attaaaagga cagacaaaac ctcatcagaa atataaaaaa gaagcgtccg aaagtgaaaa
    28801 ataggtgttg cttatgacaa acacaccatt gattttgaaa gaaattccga aggatgaagc
    28861 catttctttt attcggcaat accattattc aaagatactc cctagattgt gtaaatactt
    28921 tctagggatt ttttcagaag aaaaattatt aggcgttgtg gaattaggct ggggtacgca
    28981 acccctacaa accatacgca aactctttcc cgacagttcc cttcaaacca cagattattt
    29041 agaaattgga aagatgtgtt ttcttccaga aatgaatcaa accaattatt ttggcagtca
    29101 agctctttca gctctaatta agtggttgaa agaacatacg gactgtcatt ttttatatac
    29161 actggcagat ggaattgagg gaaaatgtgg ctatgtctat caagcgtcca acttctttta
    29221 ctgcgggtat ttcaagacaa gtgtctatcg tgataagcag tcatgggaaa agatacaccc
    29281 tcgaagtgct cgccttttat tagaggaaaa tgctcgcttt gaacaggtag agaaaaagca
    29341 ttggctttcc caggcatttt gcgaatacaa agggattgag aaaatcaatg gacgcatgtt
    29401 tcgctatctc tatccgttga caaaagaagc gaaaaaactg ttaggacaca ctctctatcg
    29461 acgtcattat tatccgaaag aaaaaaattt acgctttgaa aagcgaatcg cttatcagaa
    29521 atatgaagcc atttctcaac caacttttga taaacaggct cgtatctaca atacacaatt
    29581 attttgatag gtggaaagga ggatttttat gccattgctt catgacagag gagagcgaat
    29641 caaagccaac gataagcact tggtctatca ttttgtacta atttcattgc tggtagtttt
    29701 tctagtcgtc ctactccctt ttcattggag agcgttacaa tcggtgaact ggcaagcagt
    29761 tcagttagat acatttttct atcaacttca tacgccatac ttttggagca gtattctcac
    29821 tgctcttttt gtatgcggac ttatcagctg gctttattat cactatcgta tagattctat
    29881 aaaacagtta gagcaccgtc aaaagttagc tcgtatgatt ttagaaaatc attggtatga
    29941 agccgaagaa actcaatcag agggattctt taaagattta cccagcaaga aaaccaaaca
    30001 acgaatccgc cattttccta aaatatatta tcgaatgaaa gatggactaa tccatattca
    30061 tgtagaaatc accttaggga aatatcaaga tcagcttttg cacctagaaa acaaactaga
    30121 aacaggattg tattgtgaat tagtggaaaa aacgttgaaa gattcttatg tagagtatgt
    30181 cctactttat gatactattg gcaatcgaat tggcattgaa gacgtggtag ctcaagatgg
    30241 acgtgtcaaa ttgatggaat cggtctattg ggagttcgat tctctccctc acatgttaat
    30301 tgctgggggc actggtagtg gaaaatccta ttttattctc acattgattg aatctctcct
    30361 gcatacaaat gcaaagcttt atattcttga ccctaaaaat gctgatttgg cagacttagg
    30421 ggcggtcatg aataatgtct attaccgtaa agaagatatg ttggcatgta tcaatcaatt
    30481 ttacgaagat atgattgatc gcagtgaaac catgaaacaa caccctaact ataaaacagg
    30541 agaaaattat gcttatcttg gtttgtctgc caattttctt atttttgatg aatatgtggc
    30601 aatgatggat atgctaggtc gtgaaagtac caccgttatt cataaactca aacaaattgt
    30661 aatgttagga cgacaagcag gcttcttttt aattcttgcc tgtcaacgtc cagacgcaaa
    30721 atattttagt gatgggattc gggacaactt taacttccgt gtggctttgg gtcgtatgtc
    30781 tgaaatgggc tttggtatga tgttcggaag cgatacacaa aaacaatttt tcttaaaacc
    30841 catcaaggga agaggttacg tggatacagg aaaaagtgtg attagtgagt tttatacacc
    30901 ccttgtacca aaaaggtacg atttcttagg agaaatagga aaagtcattc aaaaaaagca
    30961 atctgagcct gtaccgcacg aagtgaaagg tacaggttca gacttgcttt gattggtgtg
    31021 gcgttagcca cgccaatcct ttagccccct ctatctaaca ggggggcaca aatacaactg
    31081 aaaacaggca attctcatca accaatgttg atggtacaaa ggattgcgga aaatatgaaa
    31141 ggtgtaaact caagggtcat agtttacatc ttttttagat tggaggtttg cgaatggaaa
    31201 acgaaaaaat ttggattaag gaattgaaag aaaagcgact ggcttatggc gtttctcaaa
    31261 ataaactggc ggttgcttct catattacca gaccttacct atcggatatt gaaacaggca
    31321 aggcagtccc tactcaacag gtgaaagaag atttactgaa cgctttggaa cgcttcaatc
    31381 ccgataaccc attggagatg ttattcgact atgtacggat tcgattccct accaatgatg
    31441 tggctcaaat tatcggtgag gttttacgtt tgaatatgga ttatatgcta cacgaagact
    31501 ttggctatta ttcttatccc gaacactatc gtttcggtga tattgttgtc ctagtctctc
    31561 atgatgtaag taaaggtgtt ttattagaac tgaaaggaaa aggctgtcgg caatttgaaa
    31621 actttctatt ggctcaacat cggagctggt atgatttctt tcaagattgc atggaacata
    31681 aaggtatttt caaacgcttg gatttagcta tcaacgataa aacagggatt ttgaatattc
    31741 cagatttggc aaagaaatgt aaacaggaag aatgcatttc cgtctttcga agctttaaaa
    31801 attatcgcag tggtgaattg gttcaccgtg atgaaaaacc atacatggga aacacacttt
    31861 atattggctc attaaaaagt gaagtctatt tctgtatata tgagaaagat tatgagcaat
    31921 acgtgaaaaa tgatgttccc ttagaagacg cagaagtaaa gaatcggttt gaaattcggt
    31981 taaaaaatga ccgagccacc caagccatga aagatttatt ggcacatcaa caggcggaaa
    32041 aaacagcctt tgaaattatc aatcgctata tccgctttgc agataaagac gacactaaac
    32101 gtcggagtga ttggaaaact aatgaacgtt gggaatggtt tatcggtaaa aatcgtggag
    32161 cattacgcct aaccacacag ccagagcctt attcttttga acgaacattg aactggttac
    32221 atcatcaagt cgcccctaca ttaaaaatcg caagcatttt agatgttctc aacggtacaa
    32281 ccattatctc caccatgatt caggaagcca aactgacaga aaaacatgag aaattaattg
    32341 aacaacaaca tctagccatg gaggatttga ttacatgagc cttttaacaa aagaattaaa
    32401 gaagttaggg tttcagtgtg gcattgagtt tcaagcgtat atacaaaata cagggaaata
    32461 tacgtctctc attattgagg gaaaacggca agctggtgat accatctata cctatgattt
    32521 ttacaaggtg agattttata ataattatac caatcgagta accgtctatg gagagcattt
    32581 aacccctttt caattattaa ggcgtgtaaa aagctatatc tattatcgag aaaaatatct
    32641 taaggaaagg aggacaatca catgaatttt gggcagaatt tgtacaactg gttcttggat
    32701 aatgcacagt cattggtatt gttagcgatt gttgtcattg gactgttttt aggttttaaa
    32761 cgagaatttt caaaactcat tggcttcttg attattgctc taatcgccgt cggacttgtg
    32821 ttcaatgcag ctggcgtgaa agatgtgtta ttaaacttat ttaaccgaat cattggtgct
    32881 taacagatat ttttcatgat aaaataggaa gtatatattt tgttatgaaa ggatttatga
    32941 atatgtttac aaatgatcaa tttaaatctc ttttaattaa aacattagaa agaaagagct
    33001 cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa
    33061 ctttagatga gactgacgat tttacttatt ataaaggata taaaaagggc tggaatacgc
    33121 tttgcacata tcttaaaata agagttcctt ttgatgattt agactttttt gaaagtcata
    33181 aagatttgat tacccagaca gcaagctcca tatatgataa acaaggtgat aatgtattag
    33241 tggatactat tttagtacca cttccagaga attacgaagt aattaacttt agtcagttga
    33301 aaattagtga tgtggtttca caagctattg aagacgctga gtcttttatg agtaatggtg
    33361 aatatcaacg tgcctttgac agagttcaca ctgcttttca tggatattta attgaaatac
    33421 taaaaaaata tgaaattaca gttcctagag acgaaaatct atctaaatta tat
//
LOCUS       DAFCLG010000017        32968 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFCLG010000017 DAFCLG010000000
VERSION     DAFCLG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.017"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            164..2698
                     /gene="secA"
                     /locus_tag="LND56_000791"
     CDS             164..2698
                     /gene="secA"
                     /locus_tag="LND56_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK6742735.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            2825..3926
                     /gene="prfB"
                     /locus_tag="LND56_000792"
     CDS             join(2825..2896,2898..3926)
                     /gene="prfB"
                     /locus_tag="LND56_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK6742736.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4031..4717
                     /gene="ftsE"
                     /locus_tag="LND56_000793"
     CDS             4031..4717
                     /gene="ftsE"
                     /locus_tag="LND56_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK6742737.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4701..5594
                     /locus_tag="LND56_000794"
     CDS             4701..5594
                     /locus_tag="LND56_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742738.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5746..7194
                     /gene="cls"
                     /locus_tag="LND56_000795"
     CDS             5746..7194
                     /gene="cls"
                     /locus_tag="LND56_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6742739.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            7531..8388
                     /locus_tag="LND56_000796"
     CDS             7531..8388
                     /locus_tag="LND56_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK6742740.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8503..9423
                     /gene="pstC"
                     /locus_tag="LND56_000797"
     CDS             8503..9423
                     /gene="pstC"
                     /locus_tag="LND56_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBK6742741.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9423..10307
                     /gene="pstA"
                     /locus_tag="LND56_000798"
     CDS             9423..10307
                     /gene="pstA"
                     /locus_tag="LND56_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK6742742.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10322..11128
                     /gene="pstB"
                     /locus_tag="LND56_000799"
     CDS             10322..11128
                     /gene="pstB"
                     /locus_tag="LND56_000799"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6742743.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11144..11902
                     /locus_tag="LND56_000800"
     CDS             11144..11902
                     /locus_tag="LND56_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6742744.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11915..12592
                     /gene="phoU"
                     /locus_tag="LND56_000801"
     CDS             11915..12592
                     /gene="phoU"
                     /locus_tag="LND56_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK6742745.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12814..13848
                     /locus_tag="LND56_000802"
     CDS             12814..13848
                     /locus_tag="LND56_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6742746.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            13845..14612
                     /locus_tag="LND56_000803"
     CDS             13845..14612
                     /locus_tag="LND56_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6742747.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            14712..16280
                     /gene="liaX"
                     /locus_tag="LND56_000804"
     CDS             14712..16280
                     /gene="liaX"
                     /locus_tag="LND56_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBK6742748.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16456..16764
                     /locus_tag="LND56_000805"
     CDS             16456..16764
                     /locus_tag="LND56_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBK6742749.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            16764..17114
                     /locus_tag="LND56_000806"
     CDS             16764..17114
                     /locus_tag="LND56_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK6742750.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17280..18218
                     /locus_tag="LND56_000807"
     CDS             17280..18218
                     /locus_tag="LND56_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBK6742751.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18233..19063
                     /locus_tag="LND56_000808"
     CDS             18233..19063
                     /locus_tag="LND56_000808"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK6742752.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            19107..20132
                     /locus_tag="LND56_000809"
     CDS             19107..20132
                     /locus_tag="LND56_000809"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6742753.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20147..21085
                     /gene="galU"
                     /locus_tag="LND56_000810"
     CDS             20147..21085
                     /gene="galU"
                     /locus_tag="LND56_000810"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6742754.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21210..21659
                     /locus_tag="LND56_000811"
     CDS             21210..21659
                     /locus_tag="LND56_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBK6742755.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            21652..22266
                     /locus_tag="LND56_000812"
     CDS             21652..22266
                     /locus_tag="LND56_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6742756.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22329..23432)
                     /locus_tag="LND56_000813"
     CDS             complement(22329..23432)
                     /locus_tag="LND56_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6742757.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23642..24643
                     /gene="ccpA"
                     /locus_tag="LND56_000814"
     CDS             23642..24643
                     /gene="ccpA"
                     /locus_tag="LND56_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBK6742758.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            24997..25392
                     /locus_tag="LND56_000815"
     CDS             24997..25392
                     /locus_tag="LND56_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742759.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            25389..26645
                     /locus_tag="LND56_000816"
     CDS             25389..26645
                     /locus_tag="LND56_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBK6742760.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            26657..26980
                     /locus_tag="LND56_000817"
     CDS             26657..26980
                     /locus_tag="LND56_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742761.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            26973..27239
                     /locus_tag="LND56_000818"
     CDS             26973..27239
                     /locus_tag="LND56_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742762.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(27402..29747)
                     /locus_tag="LND56_000819"
     CDS             complement(27402..29747)
                     /locus_tag="LND56_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6742763.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            30026..30928
                     /locus_tag="LND56_000820"
     CDS             30026..30928
                     /locus_tag="LND56_000820"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK6742764.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            31139..32914
                     /locus_tag="LND56_000821"
     CDS             31139..32914
                     /locus_tag="LND56_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBK6742765.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT        10815 a         5843 c         7066 g         9244 t
ORIGIN      
        1 ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt tcttacttta
       61 atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt aaagtctaca
      121 tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca atttgattcg
      181 ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg caaaaaaagt
      241 tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg caaaaactgc
      301 tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc cggaagcttt
      361 tgcagtcgtc cgagaagcag caaaacgtgt attggggtta tatccatacc acgtacaatt
      421 aatgggtggg atcgtgcttc acgatgggaa tatccctgaa atgcgtactg gtgaaggtaa
      481 aaccttgact gcaacgatgc cggtgtatct gaatgcgttg actggcgaag gggtccacgt
      541 agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg agctttataa
      601 tttcttagga ttgacagttg ggttgaatat caactcaaaa tcttcggatg aaaaacgcga
      661 ggcttataat tgcgatatca cttatagcac gaataatgaa ttaggatttg actatttgag
      721 agacaacatg gtcgtttacc gtcatcaaat ggtacaacgt ccgctgaatt atgccattgt
      781 cgatgaagtt gactcgatct tgattgatga agcgagaacg ccgctgatca tttcagggca
      841 agcagaaaaa tcaacagctt tatatacaag agcagacaac ttcgtgaaac gtttgaaaga
      901 agaagaagat tataaaatcg atatacaatc aaaaacaatc agtttaactg aagcagggat
      961 cgaaaaagct gaagaaacat tcggtttgga caacttatat gacattgaaa atactgcatt
     1021 gacccatcac ttggatcaag cattgagagc caactacatc atgcttttag acatcgatta
     1081 tgttgttcaa gatggaaaag tattgattgt tgaccaattt acgggtcgga tcatggatgg
     1141 acgccgttat tcagatggtc ttcatcaagc gatcgaagct aaagaaggcg tagagatcga
     1201 agacgaaacg aaaacgatgg cgacgatcac tttccagaac tatttccgta tgtacaagaa
     1261 acttgcgggg atgactggta cagctaagac agaagaagaa gagttcagag agatttataa
     1321 tattcaagta taccagattc cgacaaaccg tccggtcatc cgtgacgatc gaccagattt
     1381 gctttaccca acattacaaa gcaagttcca tgctgtcgta caagatatca aagatcgcta
     1441 ccgcaaagga cagcctgtct tagtcggaac tgttgcagtt gaaacatctg agttattatc
     1501 agatatgctg aatcaagaac gtattcctca tgaagtactg aatgcgaaaa atcactttaa
     1561 agaagcagaa atcatcatga atgcgggtca aaaaggtgca gtgacgattg ctaccaacat
     1621 ggctggtcgg ggaacagata tcaagctagg gcttggcgta agagaacttg gtggcctagc
     1681 agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc
     1741 aggacgtcaa ggagaccctg gtatgtcaca attctacttg tctttagaag atgatttgat
     1801 gaaacgattc ggttcagaac gaatcaaagc tttcttagat cgaatgaaga tcggtgacga
     1861 agatgctgtg atccaaagca agatgctgac gaaacaagta gaagctgcac aaaaacgcgt
     1921 agaaggaaac aactacgaca cacgtaagaa cgtcttgcaa tacgatgacg tgatgcgtga
     1981 gcaacgtgaa gtaatctata aacagcgtca agaagtgatc atggaagaaa aaagcttatc
     2041 taaaccactg atgaatatgg tcaaacggac aatcagtcgt gtggtagatg cgcatacgca
     2101 attagaaaaa gaaaattgga atttggaaag tatcgtcgat tttgctggca gcacgcttgt
     2161 ccatgaagac agtatttccc ttacagatat cgaaggaaaa acctctcaag aaatcaaaga
     2221 atatctaaac gaacgagcac aagaagtatt caataccaaa gcagctcaat tgaacggacc
     2281 agagcaactg cttgaatttg aaaaagtcgt gatcttacgt gtcgtggact ctaaatggac
     2341 agatcatatt gatgcaatgg accaattacg tcagtcaatc ggcttgcgtg cctatggtca
     2401 aaacaatcca ttagtcgaat accaaacaga aggttataag atgttcgaag atatggtagg
     2461 tgccattgaa tatgaagtca ctcgtatctt catgaaagct gagatccgtc aaaacgtaca
     2521 acgtgagcaa gtttctcaag gaacagcttc tcactcagaa gatggagatg ccaaagaaaa
     2581 cagtaatact agcgcgaaaa aacaaccaat ccatgtgaac aaggttggta gaaatgatcc
     2641 ttgtccttgt ggaagcggga aaaaatataa gaactgtcac ggaaaagatg caaactaatt
     2701 tctgacagca ggattggtga gacaaattct tttttaaaga atttgtctca ccttctttct
     2761 gttatttgac gattctgtta gactagatat aataaaaaaa gtatagtgaa agaaggaaaa
     2821 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
     2881 cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa gcagaagcaa
     2941 aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa
     3001 acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa ctggaagaaa
     3061 tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agtaatgcaa gaagaagcag
     3121 aagcacgtat caatgcgcta gatgaaaaaa tgaatacgta tgaattatct atgttgttag
     3181 atggtcctta tgatcgaaac aacgccatcg tcgaattgca tccaggggct ggcggaacgg
     3241 agtcccaaga ctggggcagc atgcttttac gcatgtatac acgctgggca gagcagcatg
     3301 ggtatcaagt agagacacta gattatcaag caggcgatga agcaggaatc aagagtgtga
     3361 ccctactgat caaaggacac aacgcatttg gctatctgaa atcagaaaaa ggtgttcatc
     3421 gccttgtacg tatctcgcct tttgactcag caaaacgtcg tcatacttct ttttgttcgg
     3481 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
     3541 agatcgacac atatcgggcg agtggtgcag gtggtcagca catcaataag acagaatccg
     3601 ctgtacgaat cactcacttg cctacaggaa cagtagtagc gagtcaggca caacgttcac
     3661 aattgaaaaa ccgggaacaa gcgatgggga tgctgaaagc taagctttac caattagaag
     3721 tggaaaagaa agaacaagaa gcagctgcac tgagaggaga acaaaaagaa atcggctggg
     3781 gatcacagat ccgttcttat gttttccatc catattctat ggtaaaagac catcggacaa
     3841 acgtagagac aggaaatgtc caagctgtta tggacggaga tcttgattta tttattgacg
     3901 gctatttgaa aatgcatctg caataagagc cctttgttaa attgaaataa aattgtaaga
     3961 ttttgcaagc aagttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
     4021 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaacg gtaccactgc
     4081 tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtgtatgttg ttggtccgtc
     4141 tggagctggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
     4201 aactttgaac gtcgcaggtc atgatctaat agccatcaaa aaccgcgaag ttccttatct
     4261 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
     4321 tgaaaatgtt gcgtacgcca tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg
     4381 agtaatggaa gtgctggatc ttgttggttt gaaacacaaa gtccgagtct ttcctagcga
     4441 attatctggt ggggaacaac aacgtgtttc tattgcccga gcaatcgtaa atacacctaa
     4501 agtacttatc gcggatgaac cgacagggaa tctagatccg gaaaactcat gggaaatcat
     4561 gaaactatta gatcggatca atgcacaagg aacaactgtc gtgatggcta cacataacag
     4621 tacgatcgta aatacgattc gacaccgagt aattgctatc gaaaacggcc gcattattcg
     4681 agaccaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
     4741 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt agcttcggtc agtgcggtaa
     4801 cgatcacgtt gactttagtt ggtgtgttca tggcggtcat catgaacgca accaaactag
     4861 cacaagatat agaaggaaac gtggatgtat ctgtgttcgt agacattggt acaaaagacg
     4921 aagagatgaa aaaattagaa aaagaattaa atgacttaga tcatgtcaaa aaagtcgaat
     4981 tttcaagcaa agaacaggaa ttgaagaaaa tccaaaacga aatgggcgat tcttggaaat
     5041 tgtttgaagg ggataataac ccgttatatg atgtatacgt agtaagtgct accgatcctt
     5101 cttatacaaa acaagtagca gaaaaagcgg cagatttgaa gaatgtttcc cgttctgatt
     5161 acggtggggc ttcctcagat cggattttcc aaattgctgg tgcagttcga acatggggat
     5221 tagttgcggc ggcattgctg ctgtttgtag caatgtttct catttctaac acgattcgga
     5281 tcacgatttt atctagacag cgtgaaattc aaatcatgcg tttagtaggg gcaaaaaatg
     5341 gatatatccg ctggccattt ttccttgaag gtggttggat cggcttaatc ggtgccattc
     5401 ttccgatact gatcattaca tttggttacg catggtttta ccaattgatc aatccgtcat
     5461 tattacgatc tcattattca ttgatccatc cagaaaatat cgtttggaaa atcaatcttt
     5521 tgatggtcgg tattggtgtg atcattggtt cactcggctc gatcatctct atgagacgtt
     5581 tcttgaaatt ctagtgtcac aagataaata taatcagaaa gttggacgat ttcgttccag
     5641 ctttcttttt ttatcagcta ggtggagtaa atggggcggc ttttttctct ttactttaaa
     5701 aaacaacttg ggtattctta agttggtaga aaaagaggtg aagccgtgaa gattatcgtt
     5761 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat tatcgttttt
     5821 cgagaaagaa aggaaactgc gcaaacatgg gcatggctgc tcgttttgat gttcattcca
     5881 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
     5941 gatttgaaga tccaagggaa aatcggtaag aatctagaaa tcgaagaaga taaacaagca
     6001 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
     6061 tatatgctta ctgtatttga aagtacgctt tatacgacta aaaatgaaat cactttattt
     6121 acagatggtc gtgaaaaatt cgatgcgctg atcgaggata tcaaacaggc agaagaccat
     6181 atccatttcc aatattatat ttaccgcagt gatacattag gtgaagaagt tcgagaggca
     6241 ttgacagagg cagcaagacg cggagtaaaa gtcagagtcc ttcttgatgc atggggttcc
     6301 acacaagttt cttctagttt ttttcagaat ttgaaaaaag caggtggaga agtcgcattt
     6361 ttctttcctc tattcgttcc gtatatcaat cctcgaatca attatcgcaa tcatcgtaaa
     6421 atcgtagtga ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaacgaatat
     6481 ctaggattgg taaagaaatt tggttattgg cgagacaatc atctgcgtat ctatggagaa
     6541 gcggtttaca ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
     6601 gtaggatatt caccggaatt tttcccgacc attcattcta caggtgatat ggctgttcaa
     6661 atcgtgacaa gcggaccaga taccgaacat gagcaaatca aaatgacata tttgaaaatg
     6721 atttcgttag ccaaacggga gatattgatc caaacgccgt attacatacc ggatggatcg
     6781 attcatgagg cattgaagtt ggctcttctc tcaggcgtaa aagtccatat ccaaatacct
     6841 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctgatc
     6901 gagtatgggg caagaatcga aacatatgaa aatggattta tccatgcaaa gacgatgatt
     6961 atcgatggcg gcatcgtttc cgttggttca gctaatatcg acgttcgctc atttagattg
     7021 gattttgaag tcaatacact gatctatgat gaacgaatgg cttctcgcgt aagaaaagca
     7081 ttctttgaag attcaaagat ttcgacgcat ctcacaaaag aagtgtatga aaaccgaggg
     7141 atgatgatca agatgaaaga aggattagca agactgattt cgccgctcct ttaaaataca
     7201 aaaaggacag gagagccatc aatagatgga aatctcgcgt ccttttttct cggggccatt
     7261 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
     7321 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
     7381 ttcaaagcca gtatagcata gaaactttta atgagatagc tttttttgca attatgagaa
     7441 tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa tgatagaata
     7501 aacatacttt aggattaaag gagatttcta atgaaaaaac taaaattatt cttaccaata
     7561 ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt
     7621 actgcagttg gctcttctgc cttacagcct ttagtagaaa cggttgctga aacctaccag
     7681 gcagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac tgggttgtct
     7741 caagtacagt caggagcagt cgatatcgga aattctgatt tgttcgcgga agaaaaatca
     7801 gggattgatg cttcggcatt agtcgatcat cgagtagcag ttgttggaat cactcctatt
     7861 gtgaacaagg atattggcgt aaaagatatc agtatggaaa acctgaaaaa attatttacg
     7921 ggcgagatca aaaactggag tgaagtcggt gggaaagatc agccagtagt tatattgaac
     7981 cgtgcatcag gaagcgggac ccgtagtacg tttgaaaaat gggtattgga cggagaaaca
     8041 gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccaaatcgt tgcggacaca
     8101 cccggagcaa tcagctatgt tgctttttct tacgtttccg atgatgtgga tacgttgaac
     8161 gttaacggtg tggaaccaac agatgaaaat gtgacaacga acaaatggac gatttgggca
     8221 tatgagcata tgtacacaag agaccatcca aaaaaactga caaaagaatt tttagactat
     8281 atcttatctg atgaagtaca agataatatt gtcggagagc taggctatat ccctgtttca
     8341 catatgaaag ttgaacgtga ttggcaagga aacattgtta aagagtaatc tttacacaaa
     8401 atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta
     8461 cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa
     8521 gtattattaa caaaatcaaa acgttcaaaa atggaacagc gcggaaaatt cattagtttt
     8581 ttgtgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt cgctagtaaa
     8641 ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat
     8701 tggaatccaa gccaaatagg agcaaatggg aaacccatgg taggggcatt gcctatgatc
     8761 acaggttcat ttattgtgac ctttttatct gctattgttg ccacaccatt tgctattgga
     8821 gcagcagtgt tcatgacgga gatttctcct aaaaacggaa cgaagatctt gcaaccggtc
     8881 atcgagctac ttgtaggtat tccgtctgtc gtttacggat ttatcggttt atctgttatc
     8941 gtgccttttg tcagaagtat ctttggcggg acagggttcg gtatcttagc tggtacattt
     9001 gtgttattcg ttatgatctt accgactgtc acaacgatga ctgtggatgc attaaaggca
     9061 gtgccgcgac actatcggga agcttcatta gcgctaggtg ccacaagatg gcaaacaatc
     9121 tacaaagtgg tgctgcgagc agcagtgccc ggaattttaa cagcagtcgt ttttggtatg
     9181 gctcgtgcgt ttggcgaagc cttagctatc caaatggtta ttggtaatgc ggcattaatg
     9241 ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttgacaat gggaatcgga
     9301 aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta
     9361 ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa
     9421 taatgaacgc aaaacgctca gataaaattg ctacaggtgt tctatatgtg gtttcaggga
     9481 tcatcgtatt gatcttagca gctttattac tttatatctt agttcgagga ataccgcaca
     9541 tttcatggtc attcttgact gaaccttcca aagcctacca agaaggcggt gggatcggga
     9601 ttcaattatt taactcgatt tatttgcttt tgattaccat gatcatcagt tttcctatct
     9661 ctttaggtgc gggtatctac ctttcggaat atgccggaaa aaactggctg acagacgtga
     9721 tccgtacgtc tatcgaaatc ttgagctctt tgccttctgt tgttgtcggt ttattcggtt
     9781 tcttggtttt cgttattcaa ttccagtatg gtttctcaat catttctggt gcattagctt
     9841 tgaccgtatt caacttgcct cttttgactc gaacggtcga ggaatcttta caagcggtcc
     9901 attacacaca acgagaagct ggattggcgt taggtttgtc acgctgggaa acagtgataa
     9961 aagtggttgt tccagaagcg acaccaggaa ttttgactgg tgtgatccta agttcaggaa
    10021 gaatttttgg tgaagctgcc gcattgattt atacagctgg tcaaagcgcg ccagcattag
    10081 attttacgaa ctggaatcca ttaagtgttt ctagtccgat cagcattttc cgccaagctg
    10141 aaacactggc tgtccatatt tggaaaatca acacagaagg tacgatgcct gatggtgttt
    10201 ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc aacttcggcg
    10261 ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagtaa
    10321 aatgaaaaaa tataattgga atgaaacaaa tgtcattacc atgccaaaag ataaggaagt
    10381 tgcattatat acgaatgatc tgcatgtttg gtatggcgac aatgaagcaa tcaagggagt
    10441 ggatctagaa ttcgaaaaaa ataagatcac tgcattgatt ggtccctctg gctgcggaaa
    10501 atccacgtat cttcgttctt tgaatcgtat gaatgatggg attgctaata cgaaaattac
    10561 tggaaagatt atgtataaag acgtggatat caatgcgcct caagtggatg tttatgaaat
    10621 gcgtaaacgg atcggcatgg tgttccaacg ccccaaccct tttagcaaat cgatttatga
    10681 caatataact tttgctttga aacagcatgg tgaaaaaaac aaaaagaaat tagatgaaat
    10741 cgttgaaaca agcttgaaac aagcagcttt gtgggatcaa gtaaaagata gcttggataa
    10801 aagtgccttg gctttatctg gcggacaaca gcaacgtttg tgtatcgctc gtgcaatcgc
    10861 gatgaaaccc gatatcttgc tgttagacga gccggcaagt gccttggatc ctatttcgac
    10921 aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tacacgatca ttatcgtgac
    10981 gcataatatg caacaagcag ctcgtatcag cgactatact gcctttttct atctaggaaa
    11041 agtaatggaa tatgacaaaa caaacaagat atttaccaga ccaaaaattc aggcaaccga
    11101 agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagaaattat
    11161 ctcatccaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaagg gaattgatct
    11221 aagtttcaat caaggtgaaa tcacagcgat gatcggtcct tctggttgcg ggaaatctac
    11281 ctatctacgt tcgttgaatc gaatgaacga tctgattcca aatgtaacga tcaccggaag
    11341 tgttttatat aaagacaaag acatctatgg tccgaaaaca gatgttgttg aactaagaaa
    11401 agaaatcggc atggtgttcc aacagccaaa tccatttcct ttttctattt atgaaaatgt
    11461 catctatgga ttgaagttaa aaggtgaaaa agataaaaaa gtccttgatc aagtagtgga
    11521 agaaagtctg aaggcagcgt ccgtttggga ggatgtgaaa gataaactgc ataaaagtgc
    11581 cttgtcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagtgaa
    11641 tcccgaaatc atcttattgg acgaaccaac aagtgcactt gaccctgttt ctactgggaa
    11701 aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa
    11761 tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat
    11821 cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta
    11881 tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga
    11941 attgctgaat ctccataatc agttctacga aatggggatg atggtgagca gcgctgtgca
    12001 caagtcagtt cgtgcataca tcaaacacga taaaatctta gctcaagaag ttatcgaaaa
    12061 cgatataaat atcaataata tggaaacgcg tttggagaag aaaagttttg aaatgattgc
    12121 attacaacag ccagttacaa ctgacttgcg tatgattatc acggtcatga aagctagctc
    12181 tgatttagaa cgtatggccg atcatgcggt gtccgttgct aaatccacaa tccgcgtaaa
    12241 aggacagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg attacgtgaa
    12301 aaaaatggtg gacaacgtcc tagttgcgta tgtcaaaaca gacgaaaaag atgcgcgtac
    12361 aatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaaccat
    12421 caaaaacatg caagaaaatc ctgaaacagt catcagtggc acagactatt tgaatgttgc
    12481 cagctatcta gaacgaatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt
    12541 ggctacaggg aaaatcactg aattaaatac gaaccacaat gagattttct aaactgaagt
    12601 tgtgattctg ctgtttattt aggaaaagag gttgtgaaat cagcgttttg tcctgagtat
    12661 tagagaaaca aacggtaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    12721 ggtcagctga ttgaacaccg tttattctgg aaaagggttt gtgacaaaac atgtcacaaa
    12781 cccttttcat tattccaata gaaaggataa agaatgcaaa aaaagattag catcatcatc
    12841 actctatttt ttatttgtat acttgcttca tggtttattc agcgtcaagc tgctctatat
    12901 gacgtacctg tcatcgagat cgaaagagtg agaatagaag aggatcaaca attgattaca
    12961 ggaagattga tgaatacgga ccaaaaagga gaagtgattt ctttaacggc tccttatcaa
    13021 aaaaatcaaa tcgaaacagc aaatataaca acaagacaaa tctatcttgt ccaaatgcat
    13081 ggagatgaat ggcaactagt agcaaaaaaa aatgatggct ggcttttttt ctttacaagt
    13141 gtcttcattg gcaccatgtt gcttattggt ggaaaacagg gcgtatttgc tttaggaagt
    13201 ctcatcatca acatcgtttt acttttactt ttgttgctcc tctttaccaa aacagaaggt
    13261 atttctctct taaatatagc aatcttattt gtcgttatag ggataatcgt tagtatattg
    13321 gcgttagacg gatggaatag aagcagtata atcaaaattt cggctgcagt tacaagtgtt
    13381 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagatca aggactgcgc
    13441 tatgaagaac tgaattatct gacacgaccg tatcgttctg tttttctttc cagcgtgctg
    13501 attgggacag ctggagggac actcgatacc gtgatcacag tcattgcgac attagaagaa
    13561 ttgaccaagc aacaaccgaa aatatcagcg aaacagttat ggctttcagg aaggcaagtg
    13621 ggccgagatg tgattggttc gatggctaat atccttttat tcgtctatat cagcggagcg
    13681 attccatcgc ttctgttata tttaggaaat caatggtctt tcaaagaaac ggtagaacga
    13741 catttatcat tagaactttt acgcgtcatt gctggcagct taggaatagt attatcaatc
    13801 cccatcgcgg tcctcttttt tctcgctgta aggaggttaa aaaaatgaat gtacttatta
    13861 tacttgttgg gatttttgca atcagtgtgc tatttgtcgg tggtacgcaa ggtatgtata
    13921 ttcttttagg attattcatt aatttaggaa tctttttcct attattattc ggctatcatc
    13981 agaagtggcc gatattggta ttgtcgatta tagggttcct attgatcgct gtggttatct
    14041 tattttttat caatggttat aatctgaaaa tgcgagccgc ttttgccagt atactgatct
    14101 ttttgttttg tttcctacta ttgattccta tcactgattt tttagcgatc caaggcttta
    14161 cttcgataga actagaagaa ctatctggct tagataagac gttagcaatt gattttagac
    14221 tacttacgag gtcgttgctt ctgatcagtc ttagcggagc tgttttagat gcgagtgtag
    14281 cgatttctag tggaacattc gaagtatatc aggcaaatcc gcaaatgtct tttaaccaat
    14341 tgcgccatgc tagctttgcc attgctaaaa aaatattggc atcaacagtg atgacgcttc
    14401 tttttgcgtt tatggggagt tctttagcgt tgattctttg gtttattgac ttagacattt
    14461 cttttcagca aataatcaat gaaaaatcat ttgtcttgga atatacgatg gctattctta
    14521 ccacactggc cgcactactt gtcctgccct taacttgcgt gactacagca tatctcttta
    14581 caaaaaagaa agaacatctc aagatggagt aaaagttaca acttaagtcc tatgacattt
    14641 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
    14701 aggcaatttc catgaaagaa agagaacgta ttttagactt agtgaaaaaa ggaatccttt
    14761 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaaacaaa
    14821 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
    14881 tgatcgatga acttgaaaac ggtgaagaaa aaatttctga gccagttgat ccaaaagaaa
    14941 aagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
    15001 ataaaacttc ggcgcgtcta gacgaagtaa atgctcaatt cgctgacaat aaggcagcac
    15061 gtaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
    15121 cagaagaaga attagcacag cgtcaaacgt tagaagcaga aatcaaagag ttagaagcaa
    15181 ccggcgatac actattagaa gaacaattga agctggaagc agaattaaaa gatatccgca
    15241 agaatcaatg gtctgagaaa aaagaaacat ttacggataa gtttgaattg ccagatgatt
    15301 ggaaagatca agcgacagat acgctgaatc aagtaggcga aaaaatgtca gaagctggat
    15361 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtaaatgata
    15421 acatggaatg gaaagatgtc agcttacgcg tccctggaat tgcgacaacg aaatttgaac
    15481 acgaatttta ttatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
    15541 acgtgacgtt aaaaacgtgg gacagtgatg atgtgaaagt ggaagccaag atcaaacttt
    15601 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag atcgaggtca
    15661 atgaagatca tatctccttc caaattccaa ataagcgtgt gcgtgcagac cttgttttct
    15721 acttgcctaa acgagtatac gaccatgcag ctatcaaact tctaaatggc aacatcatga
    15781 ttgagacatt ggaagctaaa gatatttata caaaatctac gaatgggaac atcatcgtga
    15841 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
    15901 acgggaacat cttagattct attatagaaa cagtaaacgg tacagtgaca tttggtgcca
    15961 caccagaaaa cctttctgtg tctttagtaa atggtgatgt acgtcttacg attaaagaag
    16021 ataatttgaa aaaagtagaa gcaagttcag tgaacgggaa tgtcaaagtt gctttaccag
    16081 atagcatcgg tttggaagga cacgctaaaa caagtcttgg aagcattaac agccgtctgt
    16141 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
    16201 gtgtttcaga tgatgagatc gcacaagtgc agctttcaac gacaacagga agtatctatt
    16261 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
    16321 gcgatttttt aaaatagctt cccctatttt aaaaaaatgt gccttattgc cgataggcta
    16381 tctcttgaac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
    16441 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
    16501 acaggtactt tagcagggat tggggaatat atcgggattg atcccactgt tatccgtgtg
    16561 ctttatgtat tcttaagctt agttgctttt ggaagtccaa tcattttata cattttatta
    16621 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
    16681 tatgaaaaaa acaattatcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16741 gatgacgaat ggagtgactt ttaatgtcgt actttcagcg tattttggtt aatacgttaa
    16801 cattcatatc gttggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    16861 ccgtccttgc cagcttcgta ttatctgtgt tgaacatgtt tgtcaaaccg attttgacga
    16921 tcctgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    16981 cgattcttaa aatgacttcc ttttttgtag gagcagaaca ttttagtttt tcttcatttg
    17041 gtgcagcgtt attcgtagct atcttgatgt cacttgtcaa tgtaattgtt agtgaacata
    17101 acagcagaaa ataaaaaaag ctgtgccgag atctcggcgc agcttttttg tttagaataa
    17161 agaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
    17221 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
    17281 tgtcagaagt tgtaaaagtt agccaattag taaaaaaatt atctttggaa atcgtcacag
    17341 gcgacgagaa aagtttagac cgcgtcatcg tgactgggga catctcacgc ccaggactag
    17401 agttgaccgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    17461 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcattatg agaagattat
    17521 gtagtgaaga tatgcccgca tttattattt caagaggatt gccggctccg gaagaaatga
    17581 tccaagcagc caatgaaaat gggatagctg tgctgcgttc gcctatctca acgtcccggc
    17641 tgctaggtga attatctagt tatttagatg gacgcttagc ccctcgaaca agtgtccacg
    17701 gtgtgttagt cgatgtctac ggattaggtg tactaattca aggggacagt gggataggga
    17761 aaagtgaaac ggcgttggaa ctgattaagc gcggacatcg attgatcgca gacgatcgag
    17821 tagatgtcta tcagcaagat gaactgacgg taatcggaga acctcctaaa atcctggaac
    17881 atctgattga gattcgaggg atcggtatca tagatgtaat gaatcttttc ggtgcgagtg
    17941 cggtacgagg ctctatgcaa gtccagttag ctgtttatct agaagcatgg gcgaaagata
    18001 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    18061 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    18121 caatgaattt ccgcgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    18181 taagtcgctt gattgaagaa aactcaggca atgactagca aggagtgaca atatgttagc
    18241 acaaatcaat cgagttgctt ttgagcttgt aggaattcct atctactggt atgcattgat
    18301 tatcgtatca ggaatcatta ttgccatgtg gctcagcagc agagaagctg ttcgggtagg
    18361 attaaaagca gatgacgtga cagattttat gttgtgggga ttgccgattt ccattattgg
    18421 tgcccgcttg tattatgttt tattcgatct tccccaatat atagctgatc caatccagat
    18481 attcaatatc cgttcaggag gattagcgat ttacggggga ttgattgcag gtgcaatcac
    18541 actgtacttt tttaccaaat atcattttat ttctttgtgg acctttttag atatcgctgc
    18601 accaagcgta ttattggcgc aggcaatcgg acgatgggga aatttcatga accacgaagc
    18661 gtatggtcca gacacgacac gaagtttctt agaaagtctg catttaccgc aattcatcat
    18721 tgataacatg tatatcgaag gaatctatcg acaaccaacc tttctttatg aatcactttg
    18781 gagtttgatt ggatttattc tgcttttggt tttgcggaag aaaccacatc ttttgaaaaa
    18841 aggagaagtc cttttatgtt atctaatgtg gtatagtttc ggacgtttct ttatcgaagg
    18901 gttacgcaca gacagccttt actttttagg tgtgatccgc atttctcaat tattgtctgc
    18961 tgttttattt gtcggcacat tgattctgtt tatttggcgt agaaaaaaag gaagtttacc
    19021 ggattatgac cgctcattag gaaaaaatca acaaattatt tgattttcag agcgttaaaa
    19081 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    19141 cttgggggac tgctttagca caagtcctcg ctgaaaacgg acatgacgtt cggatttggg
    19201 gacatcgtgc ttcacaagta gaagaaatca acacccaaca caccaatcaa cgttatttac
    19261 cagatcatca tctacccgat tccatccgag cattcaccga tatggaagat gcagtaaaag
    19321 atgcagatgc ggtactattc gttatcccga cgaaagcgat acgttctgtt gcaaaagaac
    19381 tagttgccaa attgaatacg aagccagtga tcatccacgc aagtaaggga ttagaacagg
    19441 gaacgcataa aagaatctct gaaatcttgt tagaggaaat cccgacagaa aaaagaaaag
    19501 ctgttgctgt tctttctggg ccaagccatg cggaagaagt agcaaagcat gacattacga
    19561 cgatcacagc ggcaagcaca aatgtaaaag cagcaaaata tgtacaagag ctgttcatga
    19621 accattattt tcgaatctat accaaccctg atgtgattgg tgtagagatg ggagcagctt
    19681 tgaaaaatat tattgccatt ggagctggtg ctattcacgg cttagggttt ggcgataatg
    19741 caaaagcggc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    19801 gtgccaaccc gctgaccttt attggcttaa gcggagtagg tgatctagtc gttacttgca
    19861 caagtgttca ttccagaaac tggcgcgcag gcaacttgtt aggacaaggt cagcctctag
    19921 aagaggtgct agccaatatg ggaatggttg ttgaaggtgt gaatactact caagcagcga
    19981 tggaactagc ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    20041 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatgggaaaa
    20101 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagtaaaaaa
    20161 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aggcgatggc
    20221 aaaggaaatg ttgccaatcg tggacaaacc aacgatccaa ttcatcgttg aagaagcgtt
    20281 gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    20341 tcactttgat tcaaatatcg aattagaaac aaacctaagt gaaaaaggaa aaaccgaatt
    20401 attgaaatta gtagaagaaa cgacagatgt caatttgcat ttcatccgtc aatctcatcc
    20461 aaaggggtta ggacacgccg ttcttcaagc aagagccttt attggaaatg aaccgtttgt
    20521 cgttatgcta ggcgatgata tcatggaaga cgaagtacca ttgacacagc aattgatcga
    20581 tgactatgag cgtacacacg cttctacgat tgccgttatg caagttcctc acgatgaaac
    20641 ttcaaaatac ggaatcatca acccagggga agtactggaa gatgggttat acaatgtcaa
    20701 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattgggcg
    20761 ttacttattg actccagaaa tctttgatgt attatcagaa caacaaccag gcgctggaaa
    20821 tgagatccaa ctaactgacg caatcgatac gttgaataaa acacaacgcg tatttgcccg
    20881 taaattcaca ggtaaacgtt atgacgttgg tgacaaattt ggttttatga aaacaagtat
    20941 tgaatatggt ctgacacatc cagaagtagg ggcacctttg cgtgaataca tcatgtcttt
    21001 aggtaaaaca ttagaagcag aaggaagcaa gatcaatcat aatccaaaag gaataacaga
    21061 aatcaaagaa gaaaagaaaa aataatcttt ttttctaacg agcttcagct gctaattgaa
    21121 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    21181 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg
    21241 cagcaatcgc ctttgcgata cttgtcgttt tcatcgttcg cgtattgttg caagtttcca
    21301 aaactttaga aaaagtagac cagactgtcg cagaagcaaa tacgacaatt gagatcgtca
    21361 cacaagacgt agatctttta tctagacaag tagaaggctt gctagtcaaa agtaatgagc
    21421 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    21481 atttaagtga aagtgtatcg gaacttaatc attctagtaa aaacatcgtg acgaaagtcg
    21541 gcggaatcgg aaaaacgaca gctaaagcga ctgttgccgg aaaagtcggt ggaacagcga
    21601 tgaaattttt aaaaacaaaa aacatactga aacgacagga ggaaaataag aatggctaaa
    21661 aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat cgccgcatta
    21721 ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca agcagatgat
    21781 ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc tgagttaaca
    21841 ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggggatct agcaggcaat
    21901 gttaagcaga aggtaagtga atcagcagag gaagcaggaa gcgaatcaga tgatatcttg
    21961 ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac agcagaagat
    22021 ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga agatatcatg
    22081 atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg aatgtcagaa
    22141 gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga agatgcaaaa
    22201 gaagatgcaa aagaagctaa aaaagaaatg aaagatacaa tgacagattc gactaccaat
    22261 cactaagatt actcatttct agaataaaaa aggtgagagc ggaccagctc tcaccttttt
    22321 tattttcatt actctaaaac aagtaattct tttggtgtag tcgtgaatgg cacacaacca
    22381 tcacttgtca catacacaca atcttcgata cgtaccccta ctttttcagg aatatagatt
    22441 cctggttcaa gagagaagca catcccttct tcaatcacta agtcgtttcc ttctacaaga
    22501 gaagggaatt cgtggacggt tgttccgatt ccgtgaccta aacggtgatt gaaatattcg
    22561 ccgtagccat agctgttgat cacgtttcgt gcgatttgat caagttcacc tgctgtcaca
    22621 cctgggcgaa ctgcctcttg tgcggcaagc tgtgcttcaa gagtgatgtt atagattttt
    22681 tcttggaatt cagttggttt ttgataagca actgtacgag tagcatcact gcaatagcca
    22741 ttccaaataa cgccaagatc gaataacact agttcatttg gtgaaacctt cgtgcttcct
    22801 ggtgtaccat gagggcttgc agcatttgct cctgcaagga ctaatgtgtc gaaagacatt
    22861 tgtgaaacac cttgtttttt taattggtat tcgatttctg cgataattgc ttgttcagtt
    22921 acgccttctt ttactgtttt gaagccaatt tcaaaggcaa catcggccca tttaccagct
    22981 tcgatcagtt tgtctttttc ttctaccgtt ttgatcagtt gcagacgctg gataatagaa
    23041 gttagatcgg tgtaagaatc tgtacctgtc aagatatcag agatagcatc gaagcgatcg
    23101 acagtcagag cattcttttc aatacctaca ttaccaggtt gcccatagcg tgcgcgaagc
    23161 tcttttccga tgatctccca aggattttcg ctgtctaagt agccacgtac atcgtacgcc
    23221 cagctgctgt tttgagcatc ttcaacttcc agagcaggag tgaaaaggaa aggatcttgt
    23281 tccagaggga taaaaagagc tagtacacgc tcgtgaggat cactttcata tccggaaaag
    23341 taagcgatgt ggccagggtc gctgatataa gccaattcta attggtgttg tgccatccat
    23401 tgacgtagtt cattgatttt tacgttattc atttggcaac ctactttcta aattaatttt
    23461 gtattcaata attgtaccac tgattgatta aaagaccaac gttcttaaaa ctgaaaaaag
    23521 taaaaatttt catgaaaaca gagataaaac ggttgcaatt gttgagatta tttgatatcc
    23581 tattttagaa atgtaagacg tattttcaga aaacatataa aatatgaata ggagaacaca
    23641 aatggaaaag caaacaatca ctatctatga tgtagcgaga gaggcaaatg tttcgatggc
    23701 tactgtttca cgtgtcgtaa atggcaatcc aaacgtcaaa ccagcaacaa gaaaaaaagt
    23761 cttggaagtt atcgatcgct tggattatcg tccaaatgca gtcgcacgtg gcttggcaag
    23821 taagaaaaca acaacagttg gggtaatcat tcctgatgtc agcaatatgt tctttgcctc
    23881 tttagcacgt ggtatcgacg atgtggcaac aatgtacaaa tacaatatca tcttagcaaa
    23941 ctcagatggc aacgatcaaa aagaagtcaa tgtattgaat aatttattgg ctaagcaagt
    24001 ggacggcgtg atctttatgg gtcaccatat cacagacgaa atccgtggag aattttcacg
    24061 ttcaaaaacg cctgttgtac tagctggttc cattgatcca gatgaacaag taggtagcgt
    24121 aaacattgat tatgtatcag caacaaaaga tgcagtaaat aaattagcaa aaagcggcaa
    24181 taaaaaaatc gcttttgtca gcggtgcgct gattgatcct atcaatggtc aaaaccgtct
    24241 aaaaggctat aaagaagcat taagcgaaaa caatctacca tatagcgaag gattgatttt
    24301 tgaggcacaa tacaacttta aagatggtct gtctctagtt gaccggatcc ataatagcgg
    24361 tgcaacagct gtctatgttt cagacgatga attagccatt ggtatcttgg atggtttatt
    24421 agaccgcggc gtaaaagtac cagaagaatt cgaaatcatc acaagcaaca attctttact
    24481 gacagaagta gctcgtcctc gtttgacaag tatcacacaa ccattatacg atttaggagc
    24541 ggtatccatg cgcctattga cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat
    24601 tatcttgcct tatggaatcg aagaaaaagg atcaactaaa taataaaaga tcaatcattt
    24661 aaaggttgtt cccaaagtgg tcaacttctg gaacaacctt tatacgagaa aaggggttat
    24721 gacaaaattt tgtcataacc cctttttttg tgactttaaa aaaaagagag aatggcggaa
    24781 acaaacctta cgtccatcgt atacgacaca atctgtgcgt agatgctaca ttttgttcac
    24841 tactatcaaa attactttaa aatgaaaacg tgataaaaaa gaaagtagaa aagtagaaaa
    24901 gtaactctaa aaaatccaaa aaaatccagg ctcgattgcg tacttatctt aaaggagaaa
    24961 caatccatca tacataaccc gaaaggagac ttctatttgc ctaagaaaag acgaaagacc
    25021 atagtcatca ttttgaccat tcttactata ggaatcggag gaaaatttta catggacaaa
    25081 agagaagcgc aaaaacagca agatttactc gcagtagaaa aacagtccgt caaagttctg
    25141 aaaaatacct ttgcggatat caaagaagtg aaaatcgaac aatttgagaa aaacgacatg
    25201 acaggaatct atgaagcatt gataacgatg accaatacag aaggaaaagc cgtttatttt
    25261 gattatagct ttacaaaaga aagcaatgag atagatggct atggtgttga aaatgaagaa
    25321 gtacaaaaag aagggattac ggttgatgag attatagtta tctatacaga tgggagtgag
    25381 gaaaaagtat gacaattact gataagcagt ataaaggaat aagtaataaa gtatattggc
    25441 tagatccaaa acatcctcaa tatagtaaaa gttacaaaga aggcgccatt aaaaactttg
    25501 gagaaacaaa attccaaatc ctaaaaatca aaaactcctt cgacggcatg caggcgatgg
    25561 ctgtagcgcc aatcaagaac ggtaaagtgg atatttctca agtagtgatt gcttttgcag
    25621 gaacgaactt aagcgattgg aaagatttgg aaacggatgc aagaagtgtt atgaatctct
    25681 atgggaatag accaggagca agtttttctg aaaaagcaac aacaaacaag attttatccg
    25741 ttagtggatt gaaaacagcg gggcaaatca attcagcgat tgcatttgct aatgaagtaa
    25801 aaaaggaata tggagaagct gctatttcta ctaccggcca ttcattaggt ggattcttag
    25861 cattatatgt agcagcagag aaacaatgga aaaatgtcgg cttcaacggg ccggacccgt
    25921 atgatctctt atcaccagaa gcaaaaaaat gggtggaaga aaatcccggt atgctcacca
    25981 attaccgaaa tcacgccgat tttatcggta attttggtgg taatggcaca ggtgctgaag
    26041 ttcggatcag tatggaaaca gcatacttga atctgcttgc ttcccacagt ttaacctctt
    26101 ggaagtttga tgaaaatgga aagctgatta ttcctgaaaa tgaaaacaat gttaaagctc
    26161 gaaaacaaca gaaagaaaac caagcgaccc agttgttttc ctatcagttg caagaactga
    26221 acaacatacg taaaaaacta cgattaagtg gcggccggct gacagttaat gaacaaattt
    26281 acttagatga cacacaagct aggttagtgg tggaaaagtt atcggtaaaa atgaaaagcg
    26341 ctatggaaga agttatccgc atcaaccaaa gaggaatttt tgagctagag aaaaaatggc
    26401 aagaaggctt gcggcaagca cgtgttgtcg ccccttcgtt gactgaacag gaaatcatag
    26461 aagcattagc tagtgtgggc gccacaaaag agaacttagt cgattctgcg aaagaagtct
    26521 atctgctacg aatcaataag gcaagacgta tgggggaggc ctttgataca ttggcgaagg
    26581 agatccaaga caaaatcaat gaaatcgtgg cacgagatcg agaattagca cagcaattca
    26641 attaaaggag aagtagatga aactagaaga attggaaaga gattatcgaa gtgaacgaag
    26701 acaattagaa gctcaagaag atgaattgat ccactttaag aatcaaggat tacgattgat
    26761 ggagcaagag gagcagcgac tgcatcatgt attaaagaac ttagctttag ataatgagcc
    26821 tctgtttcaa gcaagacgaa gttattcgaa ttatcaggag cattatcaac aagccctaca
    26881 agtagaacga cgaaaactct atcagcagca agacgaacta gaacgttcct atcgtcaaaa
    26941 aatcagacaa ataactatgg agaaatcatc ttatgaatga atttttaatg caattactaa
    27001 aagaattaga gaaaaatcta catcgatcaa atgtacgttc agttggtatg gggatatctc
    27061 agcaaacttg ggcagaaaaa cgattgacag atcaaaacca ccaaatgact gcagaagaag
    27121 ctactcaaga attttctaac cgacatgcac acttaaaaga atcagtaaaa gggcaatttg
    27181 cagaaaaact aaccgaatta ttccaaagac aagaaaagaa attaaaacag tttcgttagt
    27241 aaagttatat agaaaaattt tgtgaaaatg gtttttcaca tttttacata aatttgtctt
    27301 aatatcgtag atgtaagctt ttgaacacca tttattcgga aaaagggagc tgtgacaaga
    27361 tttttgtcgc agctcccttt aattctgatt aatctattag atcaatcatc tttatcgtct
    27421 gattctttgg tctctggatt cgctctagca taagctgaag cggtgttcca gtaatctgta
    27481 tagttttcat cagtaccgcc aacgccgaat ttaaaggcgc ttttcttcgg accatttttt
    27541 gcccaaagtg attccactgt ttttcctggc gtttggatag attttttatt gacagtaact
    27601 tttccagctg tttgtcctgt gaacccagat acttttttct tcatcacatc ggatgataag
    27661 tcaaatttct catcagtgcc gaaaatctca gggtttgctt ggtaagcttg attgattaga
    27721 ttggcaagat aattagccga acgtattctt gcttggctgt ccattccagt attgtcatcg
    27781 tgtcctgccc aactactgat agtgatagat ggagttgaga cgatcagcca tgaatctcgg
    27841 tattcattgg tagaaccagt ttttccgacc caatctgcat ttcctagatt tccgtttaag
    27901 cttgagatga catctttgaa tggtgtagtg attttttcat tgatcacact acgcatcatg
    27961 tcattcatga tcgatgccgt tgcttcggaa taaatacgga cagaattact ttcatgttta
    28021 taaatcacat tacctgcatt atctgtgatg gaatcaatga tatagccttc ttcgtagact
    28081 ccaccatttg ctaaggcctg gaatccattt gtctgttgaa gcgtcgttac atctactgtc
    28141 cctaaaggag ccgattcaac gccccaatta tcatttgctg ggtagttcat cttcgccaag
    28201 tagtgctcgt aagcgtattg tttcgatcca ccggaattca acacatcttg atataagtga
    28261 taagctggga tattgttgga ccattccagt gactcacgga cggtttggaa ggtgttcgac
    28321 ccttcattgg tcgcattgac gattttctca ccagcatttt ttccttcttg ccaagtcgtt
    28381 gcataatctg atacacggga ttctgatccg acaagtccca tatcgattgc aggaccgtaa
    28441 acaagaaccg gtttgatgga tgaaccagct tgacgttctg tatcaaaggc gtggttattt
    28501 tgattctggc tgtaattccg accacccacg aagccataga tacgtccggt tctgttatcc
    28561 atcaatacat ttccagtctc cacttggcta ccagcaccat catctaacat gtagccataa
    28621 ttttctacgc cggtttgcaa tgcagcatag atatctttat caatcgtcga atggatcgta
    28681 tatcctttgt tttggatcgt ttgctgtgct ttttgataat aagcttcact gaccgatgaa
    28741 tctgacatat cagcattatc tttttctgcc aattgttgtg caataatcct tgttgcttcg
    28801 ttcatcacgg tataatacaa gaattctctg tcagtctgtt ccgcgatttg ttgtgggaga
    28861 aagtcttttg taagatcata ggctttcgct tcttcgtatt gttctttcgt aatttgtcct
    28921 tcacggtaca tactgaataa aacaaaatct tttctggcaa gaccggcaga aagatcttct
    28981 tttagcgcgc ctgtattcgt gtatggacta tacgtgatag ggctttgagg caaaccagcg
    29041 atataagcag cttgcggcag agtcacatcc tttgcatcta caccaaagat tcctctagca
    29101 gcttcctgga ctccagcaat attttgcccc ttattattcc gaccaaatgg tgagacattc
    29161 aaatacgtag taatgatctc atcttttgaa aagtgttttt ctacttgtgc agaaagaacg
    29221 atctcgtttg ctttacgttt gaatgttgtt tcatcggtta ggatctgttg tttgactaat
    29281 tgctgtgtca aagtggatcc tccagacgag ccgatgccag tcgcttctga aactagtgct
    29341 cgtaaaacag ctttagggac ataaccttgg tgtttatcga agtattcatc ttctgtactg
    29401 ataatcgcgg ttttcaataa aggcgaaatc ttatcagatg aaatagtcgt ccgcatcaga
    29461 tctgtctgga tttttgatat ctctgtatta tctgcataaa caatctttga cgtttccgta
    29521 atgtttccta gttctgtctg cagttctttt ttagtcggca attgtgtgtc ttcgacaaga
    29581 taggcaaagt agccagcacc tattccgata cctagagctg cgaacaacaa aagaagaaca
    29641 gtaataaaaa cagtcaatga ttgaaacact ctaaaaataa tcttaggaac aaaccatttt
    29701 tctttttttc ttctattctg tttttttcga gtatttttgc gtggcaaaag ggtcacctca
    29761 actaatggtt ttatttcgct aattatagca aataatagga caaaaaaaca gacaaggctg
    29821 tttttgacag gaatttcttt ttcgaatgag ataaaacgtg aggaaatgat cttttttatg
    29881 aaagagttct taagcttttc tttagggaaa gtttcagtta ctatggagaa cagggcaaag
    29941 aaaaacaaac tattttcttt gtacatcatc ttatcaatat acaggaattg ttcgaaacaa
    30001 ttgcctttca aggaggaatc catccatgtt attaggctca cacgtcagca tgagcggaaa
    30061 gaaaatgctg ttaggcgcgg cagaagaagc agccagttac ggatcttcga cctttatgat
    30121 ctatacaggc gctcctcaaa atactagaag aaaagctatc gaagagatga atattgatgc
    30181 cggaaaagca tttatggcag aaaacggact aacaaatatc gtcgttcatg cgccttatat
    30241 cattaatttg ggaaatacga tcaaaccaga aatgtttcca tttgctgtac aatttctgag
    30301 agaagagatt gtccgtgcgg aagctttagg tgctactcaa atcacccttc acccaggcgc
    30361 tcatgtagga gcaggggcag aagctgggat tgctagaatt attgaggggt tgaatgaagt
    30421 tttgacaaaa gaacagacgg cacagatcgc tttggagacg atggcaggga aaggtacgga
    30481 aatcggtcga acatttgagg aattagcgca aatcatcgat ggagtaaaat tgaatgaaaa
    30541 attatccgtt acttttgata catgtcatac gaatgatgcc ggatataata taaaagaaga
    30601 tttttcaggt gtaatggaag agtttgataa ggtcatcgga ttagatcggc ttaaagtact
    30661 gcatatcaat gattctaaaa atcctcaagg ttctcataaa gatcggcatg ccaatatcgg
    30721 ttttggaaca attggatttg acacattgaa caaaatcgtc catgatccaa tgtttgacaa
    30781 catttcgaaa atattagaaa ccccatatgt cggtaaagat aaaaagaaag cttacgcacc
    30841 atatggaaaa gaaatttcca tgttcaaagc gcaagcattc gatcccgaag tgttccctga
    30901 gttattagca gaagaagtca cactttaagt tttctacctg acagcaacaa aaaaggattg
    30961 ggcagtctgc cttgtccttt tttgtttaag aaacaaagga gtgctttttg cattcgtttt
    31021 cgccatgctc cttttgcccc ctctttttaa attcagacaa acagggtata ctgatagaaa
    31081 agtaaaaaac gaaaagtttc acaaacaagt agcaaaacgt gattgaaagg acgaaaagat
    31141 gctgaacatt ttaaacccct tgatcgacca actaaaaaag aaacatacat actttattct
    31201 tggcatagcc gtattattta caattattcg gttgtattta tatagtaaag caatctatgg
    31261 aacagatatg tcggcgacct atgatgatca gctgctgatt cactatgctc aaaatatttt
    31321 ggatggtaaa tggctagggg agtacacgac tacgacactt tcaaaaggga tttcctacag
    31381 cttgttttta gtacttgcac ataaactgca tctttcttat gcagttttat tcggcatgtt
    31441 cagtattctt gccagtatat tggttgcact agcgtttaaa ccaatcgtga agaacaagta
    31501 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    31561 ttccactcga acatatcgta atgctctcgt gatcccgact gttgagatca ttatcgcttg
    31621 tttactagct atttattacc gcaaatataa ttccagcaaa aaattgctgc cgtggttttt
    31681 agggctagct gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct
    31741 tccttttact gtagtcgcgt taggcatcag tttcgttcag atcttgctag gacaagactt
    31801 ttctttcaaa gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg
    31861 gctgaaactt tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat
    31921 cgaatataaa aacgagacaa catatggtat tgcagccatc aatgaccgtt cgagtggcga
    31981 atttggacgt ttgatgaaac agctgatccg aatagatgac gaaacaaact taaataaaga
    32041 aaacagtgag atatgggtct cgcatgctgc attaaataaa gctttagacg catctcctac
    32101 atttcatact gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg
    32161 cggaaaagta aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag
    32221 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    32281 tcaagaatta gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct
    32341 gatgggatca ggggatggaa agacgatgaa tgatctgcca atcgtatttg attttctgaa
    32401 atcaggatat gcttatgcat ggcgatacaa agatttccat caagggggga attatagttt
    32461 tgggacgcaa gaacaagtcg aacaggcaca agaattattg aatcttccac taatggataa
    32521 ttggatcgat agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt
    32581 cgcgaatgtc atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat
    32641 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    32701 tagttcctct ttgattgtag tcatcggatt aatactgaca caattggttt ttcttttcgg
    32761 tgtctcgtgg ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac
    32821 tggcgcaggc gtgccattaa cgcaattcat cgcagtaaca agcatgctag tttttagtaa
    32881 attgaatctg aaaaaagctt tatcttccaa ataaaaaggg aactgtgaca aaagtcttgt
    32941 cgcagttcct ttttccgaat aaacggtg
//
LOCUS       DAFCLG010000018        31392 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFCLG010000018 DAFCLG010000000
VERSION     DAFCLG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.018"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            317..1186
                     /locus_tag="LND56_000822"
     CDS             317..1186
                     /locus_tag="LND56_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBK6742766.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1395..2420
                     /locus_tag="LND56_000823"
     CDS             1395..2420
                     /locus_tag="LND56_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6742767.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2459..3409
                     /gene="manA"
                     /locus_tag="LND56_000824"
     CDS             2459..3409
                     /gene="manA"
                     /locus_tag="LND56_000824"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBK6742768.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3503..5206
                     /locus_tag="LND56_000825"
     CDS             3503..5206
                     /locus_tag="LND56_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6742769.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5208..7241
                     /locus_tag="LND56_000826"
     CDS             5208..7241
                     /locus_tag="LND56_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6742770.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7252..7692
                     /locus_tag="LND56_000827"
     CDS             7252..7692
                     /locus_tag="LND56_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6742771.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7892..9019)
                     /locus_tag="LND56_000828"
     CDS             complement(7892..9019)
                     /locus_tag="LND56_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742772.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9022..9699)
                     /locus_tag="LND56_000829"
     CDS             complement(9022..9699)
                     /locus_tag="LND56_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742773.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9963..11729
                     /locus_tag="LND56_000830"
     CDS             9963..11729
                     /locus_tag="LND56_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK6742774.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11744..12181
                     /locus_tag="LND56_000831"
     CDS             11744..12181
                     /locus_tag="LND56_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6742775.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12194..13345
                     /locus_tag="LND56_000832"
     CDS             12194..13345
                     /locus_tag="LND56_000832"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK6742776.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13407..14105)
                     /locus_tag="LND56_000833"
     CDS             complement(13407..14105)
                     /locus_tag="LND56_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBK6742777.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14098..15450)
                     /locus_tag="LND56_000834"
     CDS             complement(14098..15450)
                     /locus_tag="LND56_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBK6742778.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15673..17073)
                     /locus_tag="LND56_000835"
     CDS             complement(15673..17073)
                     /locus_tag="LND56_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBK6742779.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17234..17971
                     /locus_tag="LND56_000836"
     CDS             17234..17971
                     /locus_tag="LND56_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBK6742780.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18096..18575
                     /locus_tag="LND56_000837"
     CDS             18096..18575
                     /locus_tag="LND56_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBK6742781.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18572..19339
                     /locus_tag="LND56_000838"
     CDS             18572..19339
                     /locus_tag="LND56_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742782.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19569..20138
                     /gene="lepB"
                     /locus_tag="LND56_000839"
     CDS             19569..20138
                     /gene="lepB"
                     /locus_tag="LND56_000839"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6742783.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20242..20844)
                     /locus_tag="LND56_000840"
     CDS             complement(20242..20844)
                     /locus_tag="LND56_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6742784.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20950..21419
                     /locus_tag="LND56_000841"
                     /pseudo
     CDS             20950..21419
                     /locus_tag="LND56_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21525..22109
                     /locus_tag="LND56_000842"
     CDS             21525..22109
                     /locus_tag="LND56_000842"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK6742785.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22112..23185
                     /gene="prfA"
                     /locus_tag="LND56_000843"
     CDS             22112..23185
                     /gene="prfA"
                     /locus_tag="LND56_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK6742786.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23178..24017
                     /gene="prmC"
                     /locus_tag="LND56_000844"
     CDS             23178..24017
                     /gene="prmC"
                     /locus_tag="LND56_000844"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK6742787.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24010..24189
                     /locus_tag="LND56_000845"
     CDS             24010..24189
                     /locus_tag="LND56_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742788.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24211..25236
                     /locus_tag="LND56_000846"
     CDS             24211..25236
                     /locus_tag="LND56_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK6742789.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25411..26655
                     /locus_tag="LND56_000847"
     CDS             25411..26655
                     /locus_tag="LND56_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK6742790.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26808..27755
                     /locus_tag="LND56_000848"
     CDS             26808..27755
                     /locus_tag="LND56_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6742791.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27889..28518
                     /gene="upp"
                     /locus_tag="LND56_000849"
     CDS             27889..28518
                     /gene="upp"
                     /locus_tag="LND56_000849"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK6742792.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28657..28899)
                     /locus_tag="LND56_000850"
     CDS             complement(28657..28899)
                     /locus_tag="LND56_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742793.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29020..29092
                     /locus_tag="LND56_000851"
     tRNA            29020..29092
                     /locus_tag="LND56_000851"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29053..29055,aa:Thr,seq:cgt)
     gene            complement(29211..29849)
                     /locus_tag="LND56_000852"
     CDS             complement(29211..29849)
                     /locus_tag="LND56_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6742794.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29972..30604)
                     /locus_tag="LND56_000853"
     CDS             complement(29972..30604)
                     /locus_tag="LND56_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6742795.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30955..31254
                     /locus_tag="LND56_000854"
     CDS             30955..31254
                     /locus_tag="LND56_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6742796.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10698 a         5488 c         6315 g         8891 t
ORIGIN      
        1 aatttttctt actactcgaa actaagcgac tttttcacaa cctcttcctt aaacggtgtt
       61 caaaagcttg catctgcggt aataagccca accaaacgaa aaataggagg agctattttt
      121 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctt tttataagag
      181 tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca ttttttttaa
      241 caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa gcttgacgta
      301 gaggggagca agaaatatga aagattttca attgggggct gccacctatt taaaaagtgt
      361 agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg ttgttggctt
      421 tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag ctggaagtga
      481 aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga tcaaaaagct
      541 gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc tttgtcgtat
      601 ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta tggggatttt
      661 gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata agaacgacca
      721 aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg aagaaaaatt
      781 cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta ctaacttaac
      841 aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg aagaagaggg
      901 catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta gcggaggcgc
      961 aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca tgatcgatac
     1021 tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt tcttcttaga
     1081 caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt ggttcgtcag
     1141 tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt ttctttctta
     1201 acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat tctagagata
     1261 gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt tttttgtgta
     1321 aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat ctgatagaaa
     1381 agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag atggaagaag
     1441 gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa acatttacag
     1501 ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg ttgtttgatg
     1561 cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta ttagaagatg
     1621 gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa aatcagacaa
     1681 tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata ccaaacagag
     1741 ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca ggtgagcaac
     1801 taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg atgctagagg
     1861 aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa ccattaggaa
     1921 tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca gaacaactgc
     1981 cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt ttggctgaac
     2041 atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt gtccaaatgt
     2101 atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg attgattctt
     2161 tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg gacttaaaaa
     2221 aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt acgtttttct
     2281 tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta catcctgtcg
     2341 atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt ttgaaagaga
     2401 aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag tttgaattat
     2461 gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag gaagtcgttt
     2521 acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt gggcgatcag
     2581 tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga aaagattaga
     2641 tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag tgtttccatt
     2701 attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc cagatgatgc
     2761 ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata tcatcgatgc
     2821 tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag aactagctga
     2881 gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta aaaaaggtga
     2941 tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca tgattttaga
     3001 aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa aagatgcgca
     3061 aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg tccctgcgaa
     3121 aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg tcacttattt
     3181 agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat ctttcaaaaa
     3241 acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg tcgttgacgg
     3301 aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg tttctgagtg
     3361 ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt tcccagatag
     3421 ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc gtccatcaat
     3481 aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca gcagtcgcgt
     3541 gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca tgccaaatct
     3601 aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt ctggcgattt
     3661 gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac ccatcttgat
     3721 cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg gtgcaattgc
     3781 ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga tgattatcgg
     3841 gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga aattcaaggc
     3901 tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg ttatttgtat
     3961 aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc tcgcctccgt
     4021 tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct tagaacctct
     4081 aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt taggaatcga
     4141 attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc ctggacctgg
     4201 tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag taaatgcgag
     4261 tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc catttgtctt
     4321 gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca cgttgatatt
     4381 tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac tcgtgatttt
     4441 tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa tcagtacatt
     4501 tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa aagagaacaa
     4561 aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg tcatttttgc
     4621 ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa agaaattagc
     4681 agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga ataaccaagt
     4741 aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa aagtacctca
     4801 aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt cacgaataaa
     4861 agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata tcacagaaca
     4921 aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg gctcacaaac
     4981 tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt ttgttatctc
     5041 aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg cacaaagaga
     5101 actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag ttgaacattg
     5161 ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg tatttatcgc
     5221 ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta tctatccaaa
     5281 aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta gataatcttg
     5341 aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc tcatttgtca
     5401 ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg aaaagtaatg
     5461 acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg acgacagagc
     5521 cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact ttttatgcag
     5581 atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt cggaaccagg
     5641 gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat gtattagaat
     5701 tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca ctgaattatt
     5761 tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc ggggaagaat
     5821 tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt ttgatgctga
     5881 cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca tataatagtc
     5941 tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc gcttctgaga
     6001 caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta aatgacttca
     6061 cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt gcttatttag
     6121 taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat gaagatgaaa
     6181 ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc gttttacaag
     6241 aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag gaagttgcga
     6301 atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc gaagaagaga
     6361 tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa gaaagcaata
     6421 tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg gaactacgat
     6481 tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct gatttaaaaa
     6541 aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta ccaggatacg
     6601 gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag cagctaaaag
     6661 aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac agcaataacg
     6721 agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa atcaatcaat
     6781 tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc gaaacgattc
     6841 gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg gttcatcgcc
     6901 ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat atggggttat
     6961 tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat ttgaaacatc
     7021 cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg ttagttatgc
     7081 tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc agtggagcaa
     7141 tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa atcgtttatc
     7201 agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa gatgatacag
     7261 ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga agcgttgacc
     7321 gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat ccaatcaatg
     7381 atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt gccacatggg
     7441 actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt gcctgatggt
     7501 gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt agtggcttta
     7561 aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc agacatcgac
     7621 caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat ttttgaacat
     7681 ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt aataacttca
     7741 accaagataa aaataagagg agctattttt gttgggaggt tcttattact gcggatgcaa
     7801 gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac ggtgacaagg
     7861 tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata tagatagtgc
     7921 ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata ccattcatga
     7981 aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc acattttgaa
     8041 aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca atcgaagaaa
     8101 acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg actagagaat
     8161 gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc cacaacaaca
     8221 ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc tcctgaccaa
     8281 tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt actagtatag
     8341 agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa atggtaaaac
     8401 ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag attacctgct
     8461 ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat aaaatcaaaa
     8521 aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag agatgcgctg
     8581 gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg agcaactcga
     8641 cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc agtcgatcca
     8701 gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc cctgtgtttt
     8761 ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc acaaattctt
     8821 tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca tagttatgat
     8881 tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg aatgcaaaga
     8941 gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag agattgcgtt
     9001 caaatgtaag taaagacata gttacacctc atataattta cgataaatag ctaaaatctc
     9061 ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg ctttagaaca
     9121 aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc cctctttctg
     9181 ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc cgttcaacgg
     9241 ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca agacatgttg
     9301 tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca tcttcaatcg
     9361 ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca aagtcaaata
     9421 atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa agaaagaccg
     9481 gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat cataagttgt
     9541 tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt tcggggcaat
     9601 caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca cacgtttttt
     9661 accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc caatataaaa
     9721 tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag ttattgtttg
     9781 catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc tgataccgaa
     9841 atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc caaagtacga
     9901 ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga gggagagaca
     9961 aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta caaaaattag
    10021 gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc tggggtgtat
    10081 taacggctct atttatccca gatggttatt taccaaatga ggctttcgct accatggtaa
    10141 gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga aaagtaattg
    10201 ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt gtcggaacag
    10261 atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat gccatcaaga
    10321 aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta gtcaataatt
    10381 tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg attggtccag
    10441 ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta tctgcccatt
    10501 tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg aataatgcaa
    10561 tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct ggaaaatcta
    10621 ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt gcttttactt
    10681 tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt cactttttag
    10741 gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta ttcttatcag
    10801 ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct ggactaagag
    10861 cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc aattcttacc
    10921 ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt tctgcagtaa
    10981 ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt caagagacaa
    11041 aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct acattctctg
    11101 gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg atgggcgctt
    11161 cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc aatcaagcaa
    11221 tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa ctacaagaaa
    11281 gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt ttgaattcac
    11341 ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca gtttctgaaa
    11401 ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc aaagagattc
    11461 tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg ctcaaagata
    11521 tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa gatttaaaag
    11581 atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta cagatggctg
    11641 cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa tacgaatcga
    11701 ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa gtttgccatt
    11761 cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc gctattgcgg
    11821 tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca tggtagatag
    11881 agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg gaacagatga
    11941 agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag atggtgtaga
    12001 ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg caggagtcgg
    12061 agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata tggacaatgt
    12121 tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag caattgctta
    12181 aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc gaggatttat
    12241 tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga atcacacgat
    12301 cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg aatcaaaaga
    12361 aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag aaaaagtaat
    12421 ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata tcttaccttt
    12481 tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa gcacaacatc
    12541 tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag aaaaagaagt
    12601 gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc caaatgcagc
    12661 agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc aagtggaacc
    12721 tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc aattaaaagg
    12781 ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta gtgtcaatac
    12841 tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca ttgatgaagc
    12901 aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa caggaagttt
    12961 gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg aaaaaatcat
    13021 tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc ggacaccatt
    13081 acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac aagaacataa
    13141 cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact atgatcctga
    13201 agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg aacaattgat
    13261 tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc aaaacgtgaa
    13321 ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt gacgaatttt
    13381 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
    13441 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
    13501 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
    13561 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
    13621 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
    13681 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
    13741 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
    13801 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
    13861 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
    13921 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
    13981 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
    14041 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
    14101 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
    14161 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
    14221 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
    14281 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
    14341 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
    14401 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
    14461 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
    14521 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
    14581 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
    14641 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
    14701 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
    14761 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
    14821 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
    14881 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
    14941 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
    15001 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
    15061 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
    15121 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
    15181 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
    15241 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
    15301 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
    15361 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
    15421 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
    15481 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
    15541 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
    15601 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
    15661 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
    15721 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
    15781 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
    15841 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
    15901 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
    15961 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
    16021 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
    16081 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
    16141 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
    16201 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
    16261 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
    16321 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
    16381 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
    16441 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
    16501 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
    16561 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
    16621 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
    16681 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
    16741 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
    16801 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
    16861 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
    16921 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
    16981 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
    17041 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
    17101 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
    17161 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
    17221 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
    17281 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
    17341 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
    17401 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
    17461 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
    17521 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
    17581 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
    17641 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
    17701 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
    17761 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
    17821 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
    17881 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
    17941 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
    18001 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
    18061 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
    18121 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
    18181 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
    18241 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
    18301 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
    18361 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
    18421 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
    18481 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
    18541 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
    18601 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
    18661 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
    18721 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
    18781 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
    18841 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
    18901 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
    18961 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
    19021 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
    19081 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
    19141 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
    19201 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
    19261 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
    19321 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
    19381 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
    19441 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
    19501 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
    19561 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
    19621 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
    19681 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
    19741 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
    19801 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
    19861 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
    19921 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
    19981 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
    20041 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
    20101 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
    20161 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
    20221 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
    20281 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
    20341 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
    20401 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
    20461 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
    20521 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
    20581 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
    20641 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
    20701 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
    20761 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
    20821 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
    20881 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
    20941 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
    21001 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
    21061 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
    21121 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
    21181 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
    21241 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
    21301 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
    21361 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
    21421 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
    21481 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
    21541 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
    21601 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
    21661 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
    21721 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
    21781 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
    21841 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
    21901 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
    21961 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
    22021 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
    22081 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
    22141 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
    22201 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
    22261 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
    22321 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
    22381 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
    22441 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
    22501 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
    22561 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
    22621 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
    22681 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
    22741 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
    22801 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
    22861 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
    22921 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
    22981 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
    23041 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
    23101 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
    23161 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
    23221 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
    23281 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
    23341 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    23401 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    23461 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    23521 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    23581 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    23641 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    23701 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    23761 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    23821 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    23881 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    23941 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    24001 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    24061 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    24121 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    24181 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    24241 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    24301 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    24361 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    24421 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    24481 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    24541 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    24601 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    24661 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    24721 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    24781 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    24841 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    24901 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    24961 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    25021 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    25081 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    25141 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    25201 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    25261 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    25321 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    25381 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    25441 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    25501 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    25561 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    25621 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    25681 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    25741 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    25801 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    25861 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    25921 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    25981 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    26041 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    26101 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    26161 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    26221 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    26281 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    26341 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    26401 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    26461 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    26521 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    26581 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    26641 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    26701 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    26761 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    26821 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    26881 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    26941 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    27001 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    27061 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    27121 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    27181 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    27241 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    27301 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    27361 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    27421 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    27481 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    27541 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    27601 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    27661 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    27721 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    27781 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    27841 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    27901 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    27961 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    28021 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    28081 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    28141 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    28201 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    28261 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    28321 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    28381 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    28441 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    28501 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    28561 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    28621 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    28681 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    28741 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    28801 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    28861 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    28921 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    28981 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    29041 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    29101 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    29161 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    29221 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    29281 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    29341 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    29401 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    29461 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    29521 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    29581 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    29641 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    29701 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    29761 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    29821 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    29881 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    29941 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    30001 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    30061 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    30121 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    30181 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    30241 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    30301 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    30361 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    30421 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    30481 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    30541 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    30601 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    30661 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    30721 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    30781 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    30841 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    30901 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    30961 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    31021 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    31081 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    31141 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    31201 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    31261 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    31321 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    31381 gtattttatc ct
//
LOCUS       DAFCLG010000019        29858 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFCLG010000019 DAFCLG010000000
VERSION     DAFCLG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.019"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..156)
                     /locus_tag="LND56_000855"
     CDS             complement(<1..156)
                     /locus_tag="LND56_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6742797.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGI"
     gene            complement(175..453)
                     /locus_tag="LND56_000856"
     CDS             complement(175..453)
                     /locus_tag="LND56_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742798.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            607..1302
                     /locus_tag="LND56_000857"
     CDS             607..1302
                     /locus_tag="LND56_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6742799.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1744..2541)
                     /locus_tag="LND56_000858"
     CDS             complement(1744..2541)
                     /locus_tag="LND56_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBK6742800.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2538..3722)
                     /locus_tag="LND56_000859"
     CDS             complement(2538..3722)
                     /locus_tag="LND56_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6742801.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3747..4994)
                     /locus_tag="LND56_000860"
     CDS             complement(3747..4994)
                     /locus_tag="LND56_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6742802.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            5209..6954
                     /gene="dnaX"
                     /locus_tag="LND56_000861"
     CDS             5209..6954
                     /gene="dnaX"
                     /locus_tag="LND56_000861"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK6742803.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6974..7288
                     /locus_tag="LND56_000862"
     CDS             6974..7288
                     /locus_tag="LND56_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK6742804.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            7313..7909
                     /gene="recR"
                     /locus_tag="LND56_000863"
     CDS             7313..7909
                     /gene="recR"
                     /locus_tag="LND56_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK6742805.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7973..8617
                     /locus_tag="LND56_000864"
     CDS             7973..8617
                     /locus_tag="LND56_000864"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK6742806.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8632..8961
                     /locus_tag="LND56_000865"
     CDS             8632..8961
                     /locus_tag="LND56_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBK6742807.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8975..9913
                     /gene="holB"
                     /locus_tag="LND56_000866"
     CDS             8975..9913
                     /gene="holB"
                     /locus_tag="LND56_000866"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK6742808.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9949..10773
                     /locus_tag="LND56_000867"
     CDS             9949..10773
                     /locus_tag="LND56_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBK6742809.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10766..11113
                     /locus_tag="LND56_000868"
     CDS             10766..11113
                     /locus_tag="LND56_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBK6742810.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            11182..12054
                     /gene="rsmI"
                     /locus_tag="LND56_000869"
     CDS             11182..12054
                     /gene="rsmI"
                     /locus_tag="LND56_000869"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK6742811.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            12163..13284
                     /gene="dinB"
                     /locus_tag="LND56_000870"
     CDS             12163..13284
                     /gene="dinB"
                     /locus_tag="LND56_000870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK6742812.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(13338..13940)
                     /gene="nrdG"
                     /locus_tag="LND56_000871"
     CDS             complement(13338..13940)
                     /gene="nrdG"
                     /locus_tag="LND56_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBK6742813.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(14255..16444)
                     /gene="nrdD"
                     /locus_tag="LND56_000872"
     CDS             complement(14255..16444)
                     /gene="nrdD"
                     /locus_tag="LND56_000872"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK6742814.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            16743..17537
                     /locus_tag="LND56_000873"
     CDS             16743..17537
                     /locus_tag="LND56_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742815.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            17527..19569
                     /locus_tag="LND56_000874"
     CDS             17527..19569
                     /locus_tag="LND56_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742816.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            19850..19999
                     /locus_tag="LND56_000875"
     CDS             19850..19999
                     /locus_tag="LND56_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBK6742817.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            20010..21524
                     /gene="dltA"
                     /locus_tag="LND56_000876"
     CDS             20010..21524
                     /gene="dltA"
                     /locus_tag="LND56_000876"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBK6742818.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            21521..22726
                     /gene="dltB"
                     /locus_tag="LND56_000877"
     CDS             21521..22726
                     /gene="dltB"
                     /locus_tag="LND56_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBK6742819.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            22771..23004
                     /gene="dltC"
                     /locus_tag="LND56_000878"
     CDS             22771..23004
                     /gene="dltC"
                     /locus_tag="LND56_000878"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBK6742820.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            23007..24275
                     /gene="dltD"
                     /locus_tag="LND56_000879"
     CDS             23007..24275
                     /gene="dltD"
                     /locus_tag="LND56_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBK6742821.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            24416..25498
                     /locus_tag="LND56_000880"
     CDS             24416..25498
                     /locus_tag="LND56_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBK6742822.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            25498..26301
                     /locus_tag="LND56_000881"
     CDS             25498..26301
                     /locus_tag="LND56_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6742823.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPMGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            26371..27111
                     /locus_tag="LND56_000882"
     CDS             26371..27111
                     /locus_tag="LND56_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6742824.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            27122..28126
                     /locus_tag="LND56_000883"
     CDS             27122..28126
                     /locus_tag="LND56_000883"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBK6742825.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(28171..>29858)
                     /locus_tag="LND56_000884"
     CDS             complement(28171..>29858)
                     /locus_tag="LND56_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742826.1"
                     /translation="KDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLD
                     EANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSS
                     PYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKR
                     LEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRM
                     FYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEA
                     YKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLID
                     HILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQ
                     TKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELP
                     INDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLF
                     SRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9435 a         5255 c         6128 g         9040 t
ORIGIN      
        1 gatcccagaa gcaaaacctg cttggctcaa gctggcgtgt aaggtgtctt gcgcaatgac
       61 tgccatgatg accatcaaca gataataaat caggtagaca ataaaattga tcaatgtgat
      121 tccaataaaa tatttattaa ataatttctt ttccatgtat gttatacatt ccccttaagc
      181 tgaatatgct tttaattttt gtatttgaac ttctacatcg tgataattgg atggatagcg
      241 atcaacaagt tggcgaataa ataaatctct tgtagacata aacagattta aaaattcttc
      301 gcattcgttt gttggattct catccactgc tttgatcaat tcgatcgagt tttgatacgc
      361 atcatttaaa aagactaaag aagctttcat atcaaatctt gtttgatatg ctgtttctaa
      421 tcgattaatg attgtgttca tccagtaatt catgtttttt ccctctattc ttaaaaaatt
      481 ttacttctgc agtataacga ataaaaaaaa ggaaaaaaat cgcataagtg atggaaaaag
      541 gtacttaaca aatgttaata tcattagtaa aatacctttg gactttaaaa agttttaaag
      601 tactgtatga tctttttaga tgaaagtttg gatgggggag catcattgga tagtagagtc
      661 ttaaaggaat atttggataa aaatgatttt ccgattatac gcaaaggata tcataaatat
      721 ttgacattcg aagggatgga agattcccat acgtatatct tgaaagaggg aacggtaaaa
      781 gcaagtgtta tttcaaatga tgggagagaa tttaaccttc gctatatcaa agatcttgaa
      841 atcgtctctc ttttaaaaga cgaatattca caattcatcg attcgccgta taatattcga
      901 gttgaatcag aatatgcaga gttttatcga atcaatcgtg tgcgcttttg gcaaaatatc
      961 aaccaagacc caaagctaca acactatgta aaagagtatt accgctatcg tctgctttat
     1021 tcgatgaaaa aaatgcagca aatgctatta aacggaaagc tgggagctgt ttgtacgcaa
     1081 ctttacgaat tatatgattt attcggtgtg cggacagaag aagggatgct gatcgatttt
     1141 tcgatcacga atgaagaaat cgccaaattt tgtggcatct caactgctag cagtgtaaac
     1201 agaattttaa aacaactgaa aaaagatggt attatcaccg ttaaagacca tcatatcgtt
     1261 attttgaata tggacttgtt agaagacaat attatctttt gatgaacaaa gaggttgtga
     1321 aatcagcgtt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
     1381 ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttacca agaggttgtg
     1441 agcctgccgt ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg
     1501 atgaatattg tgacttgtga ccgaggagtt ggtaggagaa caccgtttac caagaggttg
     1561 tgaaaaagct gttctgcatc aagtaataag aagagccaag taaaaacagt ctcttatgtt
     1621 tttaacttgg ctggacttat caccgtagat gtaagctttt gaacgccgtt tatttgcaac
     1681 aaaaggggct gtgacacgat ttttgtcaca gccctttttt tgcgaacatt catttagcaa
     1741 tttttagaaa cttctgccgc caccgccaaa agtcccgcca ccgctagaat gggtagtact
     1801 tccgccaccg ccgcttccag gaggtgggct tttggggatt cttcttgtcg tgacaaaaga
     1861 atttgtcaga cgatcggttt tttcagttaa tttgatactg gatttttctc ggaatggata
     1921 tttgtagatg cctgatttca attgataacg cgaaacagtc acaaaataaa agacaagact
     1981 aaggataaga gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa ccttatagcg
     2041 tgtaattttg ccagtttctt cgtctacacg ataatgtcct cctggaactc cgtcttccac
     2101 atatgttgct gatttagaca ggtaagcttt tgcagcattg taataggcag catttgtcat
     2161 ctgatcataa acatcatcta agatcgaatc gattctgcta tcagtcaaat aatcgatcat
     2221 attaccagaa gtcgaaatgt agatttcacg ctgtcccata tctaatagca atactgagcc
     2281 attttgatca tttccgactg cgtctcttaa gtaattatca gcaaagtctc ttggttcttc
     2341 tgaattagat gtagtcgtta cgatcaagac tcgtcctttg attttttcat tcagcttttc
     2401 tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat aacccagcct catcatttac
     2461 cgtagctgta tctgcatgag tagccaccgg ccagccaaca cttactagca aaagaaagaa
     2521 taaaaaaaat tttttcatca tatcaagtaa cctccgatca gaaagaggat cgctagccca
     2581 gcgaaaatcc caagagcggt cagtcccaat tttttctggc tgatcggcag gacaccgctg
     2641 acctttcctg tttgtccatt cattgcgtag tagtagactt ttttacttga gtcattgctt
     2701 cgataagtaa caagccatac cggcagtaat acgtaattat ttacttcgtt tgtgattgaa
     2761 gcagaagttc gcacaccggt caatgttgta tatccatttg cggtatcttt taacagcttc
     2821 tctgaatagt cttttaattc agattgaatc tgtgatttga ttgcctcata ttcgatatct
     2881 cttttttctg cttggaaccc ggctaaatac tggcttttaa aatcaattgc tttatctaac
     2941 gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat tttttgataa ggcatttttt
     3001 actagttctt taaaagaaag cttgcctgct cttgaaacag caaattgctt cgtttctgtg
     3061 tattcgatgt cacctatccg ccatatgcgg ataactgtac cattcgcctg catggcacca
     3121 tctacttcag catctgtagc ccaataaggg aaatacacac ctgtcaactt atcgatttga
     3181 tccttattga aaaaagcttt gggaacaaac cattttttct tggtccaagc gagaaatctg
     3241 ttgattgctt cttctttctc gacagcaaaa ggaagtactt tatttggcaa gaattctccg
     3301 cttaaacgtc ccgatagtac gacgggatta tggcagtagt aacaataagt cgcagcagtt
     3361 gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg
     3421 gtattatctg cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg
     3481 cgctcggctt cattcatatc ggcaagctgt tcttgggctg taaaagtaaa atcttcggta
     3541 ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg cttctttttg cttttgctca
     3601 tattggctga cttcttcttc tgtatagata ttcaggcagt attcgcaatg aaatttttgg
     3661 tctttgggat caaaagtcaa cggaccgccg caattaggac atttatgtgt aagaacatcc
     3721 atcagctgca cacctttcta aataaattaa ttaacgggga ttcctttaaa cggtttacca
     3781 cagtttggac agaatttagg gatgccgttg gaaagatcca cgatctcatt gcattcactg
     3841 catcgcattt gtagcttagg ctgaacgttt tctacaggtt tggcagctcc gcagttggaa
     3901 cagaattttc cggaattttc tgtcccgcaa actggacatg tccaagtatc cgcatttcct
     3961 tgtgcagttt gttgattttg tttttctgct tgttgtttga tttgttcttg gttattttga
     4021 gctgcttgag aaagataaga accatttgca ttcattccca tacctacacc cataaagccg
     4081 gtcattgctc ctgcttcatt ttttccggct gcttccatac cacgtgcaat ggaaccttgc
     4141 acgtatcctt cacgtacgct aggatcacct agcatcgccc cttcgttacg catattgatc
     4201 agtttcacag aatcatcggt gtaagaaatg ctggctacag ccacagaaac aatctccatc
     4261 ccacgtaatt cacgccaaga atcatccaat gcagtatcca tgtatttgct taattccaaa
     4321 ctcttagatg gtacataaga aatccgttga ccatctgcag acatctgatt gattgctgat
     4381 tgtagcgctg ttaggaattc agctaaatac tgctcattga tatcattgat ttccacttgt
     4441 gttttgtttt tagggattgc gtttgtataa aataaaatcg ggtcaacgac atgaatagaa
     4501 taagtgccat gtgcccgtaa gaataattct gcgttataga aattgtcaaa gtagttcaac
     4561 ggcgcgcttg taccgaattt gattcccttt atttcttgga gattgatata aaaaacttgt
     4621 tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc aattgcttct
     4681 ttcagtgttc cattgaacat ggaaggagct gcgtcatttt taatggtgta ataaccttcc
     4741 tcagcggtat aatcgatgat ttttccacca tcgaccaaaa gcatcatcat gtttggatag
     4801 acatggacga ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt ggagccacgt
     4861 ttgtcatctt tgcggacttt gacacctttt gtcatgaccg ttgtatcact catattgtct
     4921 ggttcgatga cttctagcca ttgatctgcc agaccgccgc caaccatact tgttgctgct
     4981 ttgataagac ccataaaaaa ttcctcctct gattcagcta gtgccaaaca attattgttt
     5041 atttattata ccataggaag atgtgaaact tctcggacta gagacggaat tgaaatggaa
     5101 aattgcgtat tgtttaaata cctgaaaaag ccgagaagga tagtagtttt tttacctaat
     5161 atcacgtata ataagagata gtgaattttt taaaaaaggg ggatccttat ggcatatcaa
     5221 gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg
     5281 acacagacac taaaaaatgc tattgtttcc cacaaaacct cacatgccta cttgtttaca
     5341 ggacccagag gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt
     5401 ccgaacagta aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct
     5461 ggtacgcaag aagatgtgat cgaaatcgat gctgccagca ataacggagt cgaagaaatc
     5521 cgatttatcc gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa agtgtacatt
     5581 attgatgagg tacatatgct ttcaacagga gctttcaatg ccttgttgaa aacattggaa
     5641 gagccaaaag aaagtgtcat attcatctta gcaacgaccg aacctcacaa aataccagct
     5701 acgatcattt cacggactca gcgatttgat tttaaacgga tcaatacggc agatattgtc
     5761 gaacatcttg aatttatctt gaaagaatcg actatcagct atgaaccagc tgcattgaat
     5821 gtgattgccc gttctgcaga aggcgggatg cgggatgcat tgagtattct tgatcaggca
     5881 atctcgttta gtgaaggaac aattacttta gatgatgcca tgcaagtgac aggtagctta
     5941 actgacgaaa tgatggacag ttacttgagt gcttgcgtga aaaaagaagt accagaagca
     6001 ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat
     6061 atgttacaat actgccgtga cttgctgatg tatcagcaag cacctaacct agttgaggaa
     6121 caatcgagta cgatcagaag tacatttaca gaattggcac aaactgtttc agcggatcag
     6181 ctgttttcaa tgataaagat tttgagcgat actcaaaatg aaattcgttt taccaatagt
     6241 gccgctattt atttggaagt cgctacagtc aaacttgcac agctaccgtc tgcttctgtt
     6301 ccttcagcag atgtagcatt tgcagatgat cagatagtaa aacagctaca acaagagatt
     6361 aaggaactaa gaaacgaaat caaggaacta cgcagtaata caggaattga gaatacaaaa
     6421 gtacagccgg tacagcaaac aaatcataaa aaacagacaa gtacctatcg tgtaccgacg
     6481 gaacgagtat ataaagttct gcgggaagcg acaagacagc atctgaacca agtaaaagaa
     6541 gtatgggaag acttgctgat gagtctatct gtcactcagc gagcaatgct taaagcaagc
     6601 gaaccagttg cagctggacc tacaggacta gtgattgcgt ttgattatga gattgtttgt
     6661 caaagagcag caaatgacga agaattgcag ctttcggtac acaacaactt aagccgaatg
     6721 atcaaagatt atgcgcctga tcctgtttat attacaagag aaagctggcc agaacttcga
     6781 agaaattatt tgacgcaggc acaagaagag ggaacagaaa actcttttgc ttctgaagac
     6841 tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag taccagaaga aacacttgta
     6901 gtaaaagcag aggaactgtt tggctccgaa gctgttacag tggtagatga ttaataaaaa
     6961 ggagtagata attatgatgc gtggaatggg caatatgcaa ggcatgatga aacaagtaca
     7021 aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta aataaaacag agtttacagg
     7081 taaagccacc aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat
     7141 ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag atgcttcagg atcttgtcgt
     7201 aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa aattagggaa
     7261 atacgcaaaa ggtttgccag gattttagtt ttaaactgaa ggagtaatga atatgcaata
     7321 tccagaacca attgcgaaac tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa
     7381 aacagcgaca agactcgcct tttatacgat cgatatgaaa gatgaagtag taaatgaatt
     7441 tgctaaatct ttgttaagtg taaaacgcga tttgcatttt tgtagtatct gcggaaacat
     7501 cacagaagaa gatccctgcg aaatatgtag aaatccttca cgtgatcgaa gcgttatcct
     7561 agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa atgcgagaat atcacggact
     7621 ttatcatgtt ctccatggtg tgttatcacc gatggaaggg acaggtccag aagacatcaa
     7681 tatttcttct ctgttgcagc ggctgcatga tgcagatgta aaagaagtga ttatcgcaac
     7741 aaatgcaaca acagagggag aagcaacagc tatgtatctc tcacgtctga tcaaacccgc
     7801 aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta ggctctgata tcgagtatgc
     7861 agatgaagtt actttattga aagctgtcga aggaagacgt gagttataaa tttctaatta
     7921 agtgtttttt cagatacaat ataaaagcag cacaagagat gggggcaaca atgtgaacgg
     7981 actatttatt acaatcgagg ggcctgatgg tgcaggaaaa accagcatct tgaatgaact
     8041 ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt caaacaagag aaccaggagg
     8101 cattccgatt gctgaagaga tccgagcagt gatccttgat ccaaaaaatg atcggatgga
     8161 tgaacgtacg gaagcactgc tttatgcagc agcaagaaga caacatctag tcgaaaaggt
     8221 tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg acagttctct
     8281 tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt tgaatgagtt
     8341 tgctacagaa ggtactacgc cggattttac cctttatctt gacgtagatt cagacaccgg
     8401 attaagaaga atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt cggaaggttt
     8461 ggaattccac cagcgtgtac gtcatgctta cttaaagctg gcagaagaga atccggaaag
     8521 aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac
     8581 aattattgaa cagtatccgc aattttttga aaactaggaa ggtgaacgtt tatgaaaatt
     8641 attttagcga ttatccaaga caaagacagc aaccgtttat caaatgaatt gattgatgca
     8701 aatatccgtg ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg
     8761 tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg
     8821 tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac tggatatttc gatggatggg
     8881 ggcgtccctt atccagtaga agtagaagta ggcggtgcca cagtcttcgt cttacctgtt
     8941 gaaggattcc atcaatttta ggaggagctg agctatgaat caagaaataa acttggctca
     9001 aatgcaaccg atcgtgtatc agcagcttca acggagtttt gagcatgggc gtatcgctca
     9061 tgcttatctt tttgaagggg agaaaggaac aggaaaacat gaagtaggta tatggcttgc
     9121 acagcacctg ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat gcaacaattg
     9181 tcagcggatc gaaaagaaag aacatccaga tgttttggtt attgaaccgg aaggacaaac
     9241 catcaaagtc gatcagatca gacgtttgca gacggaattc agtcgcagcg gttacgaatc
     9301 aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag
     9361 tttgttgaaa ttcttggaag agccccctgg agatttttta gcggtgttag aaacagatgc
     9421 agtaggaaga attctcccta ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact
     9481 ttcgaaagaa gcactgatcc atcgattgca agaagagcag attccattgg aaaaagcaaa
     9541 acttttggct ttcttaacaa acagtttagg aaaagcagtt gaaatctctc aagatgaatg
     9601 gtttaatgat gctaaggact tgatttatca atggttcgtt tacttgcaaa agcaagatac
     9661 tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca
     9721 gtttacagga ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc
     9781 aaacggcgaa tatggcaaag tgaaacagat aaaccagata attgaacgga tattgatcgc
     9841 agagcagaaa ctacgcagca atgtcagttt tcaggctgtt gcggaacagt tcgttttgca
     9901 tacgattttt tagcttgccg caaaggatga tgatcaagga ggatggaaat ggtagaagta
     9961 gtaggtgtcc gatatagaga tgcaggtcat atctattatt ttgcaccagg agactctgaa
    10021 tatgtttata gtgacaaggt attagtcgaa tctcagcaat ccttacatat tgctactgtt
    10081 gtccttccta aaaaagagat tgatcccaat gaccttcctg aagctatccg tccgatcttg
    10141 cataaagcgt cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat
    10201 gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa
    10261 gccgaataca catttgatcg aaataaattg attttttctt tcacagcgga cgggcgaatt
    10321 gactttagag aattggtaaa agatctggcc gctgtatttc gtactcgtat tgagcttcga
    10381 caaattggtg tccgcgatga agcaaagata ataggcggaa ttggtccatg tgggcgtcca
    10441 ttatgctgtt cttctttttt aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat
    10501 caaagtttat ctttgaatcc aacaaaaatt tccggtttat gcggacgctt gatgtgctgt
    10561 ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag aactccctga ctttggaaaa
    10621 gtaattgcta cacctgatgg taaagggaaa gtcgtagggt tgaatctgct tagccgtatc
    10681 gttagtattc ggctgactgg acgggaaaat cctgtcgaat atgaatggga tgaattgaaa
    10741 gaatttatcc aagtaggtga gatgaatgga taagagatca ttatacgacg gattgaatca
    10801 attagaatca gagttgagaa atacattaac agaacttgtt gaaatgaagc atgctttgca
    10861 tgagatcgta gaaaaaaata caactttgga gatggaaaat caacgtctgc gggaacacct
    10921 gcgggaattg aaccaagttg cacaagctcc agctgaaaat gtgaagcagg aactttctaa
    10981 gtctcgtatg aatcttgaaa aaatttatga agaaggattc catgtctgct acgatttata
    11041 tggatcaaga agggacaatg acgagccttg tgcgttttgt ctagaagtga tctatcgcga
    11101 aagcggtcga tgaccaataa caagaggaga ctggagcgga gaaatactgc tccagtcttt
    11161 ttattaagga gattgtgaca gatgtataat caaaaaagtt tcaagggaaa tccggtatat
    11221 ggggatttat atcttgtacc gacgccaatt ggaaatttag aagatatgag ctacagaagt
    11281 gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag
    11341 ttattgaatc attttgaaat ccatacaccc caaaaaagtt tacatgaaca taattataaa
    11401 gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt
    11461 gacgcaggaa tgccttccat cagcgatcct ggacatgagc tagttttagc ctgcataaaa
    11521 gaagggattt ctgtgatctc tatcccagga cctacagctg ggatgacggc tttgattgct
    11581 tcagggctag tgcctcaacc atttttattt tatggcttcc ttccacgtaa gaaaaaagaa
    11641 cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta cgctgatttt ttatgagtca
    11701 ccttatcgga tcactgctac attgacgaat ttcttagaaa tttttggaaa tcgccaagtc
    11761 gttttgtgtc gggaattgac aaaggtccat gaagaatacc ttagaggaag tacggaagag
    11821 ctactcgctt atatcgaaga acatccagta aagggtgaat gttgtctgct tgtagaaggg
    11881 aatttgttcg aaatgccagt gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa
    11941 gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg
    12001 aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc atgaattaga ctgagatagt
    12061 ataagttgtc tataatgaaa gactaatgag gaaatcagct actaactttt caaatctctt
    12121 gaacgcgaac gaatgttctt gtataatgaa taagaggtga ggatgatgga gctgcaattt
    12181 cctttacata atgatacatc aagaaaaatc attcatattg atatggatgc ttttttcgct
    12241 tcggttgaag aacgagatca tcctgagttt cgcggacatc cattagtaat tgccagacac
    12301 cccagtgaca ctggtgggcg tggagtagtg actacagcaa attatgaagc aagaaaattc
    12361 ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt
    12421 aagcctggcg accatcataa atacagcgat atttccaaac aagtacggga agtattctat
    12481 cgttatacag atattgtgga accagttagt atcgatgaag cgtatcttga tgtgaccgaa
    12541 aataaaatca attgcaagtc agccatcaaa atcgcccgaa tgatccaagc agatatttgg
    12601 gagagtgtcc agctaacatg ttctgcggga gtaagttaca ataagttttt ggccaaatta
    12661 gcatcagact atcaaaaacc tagaggaatc acggtagttg cccctcaaga cgctgtgcca
    12721 tttttaaaag ctttgccaat tgaaaaattt catggggtag ggaagaaaac tgtacctaga
    12781 atgcatgagc tagggattta tacaggaaaa gacctatatg aatgcacaga aatgatgttg
    12841 attcgaaatt ttggaaaaat gggctattct ctttatcgta aagtcagagg aatccatgat
    12901 tctccagtaa acgttacaag agagcgaaaa tctgtgggaa aagagcatac ttatgggcag
    12961 ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc aactagctga aaaagtagaa
    13021 gaagcactga gaagagtcca aaaacatggg aaaacagtag ttttgaaggt caggtacacg
    13081 gactattcta ctgtgaccaa aagagtgaca ttgccagaat atatctataa aaaagaagca
    13141 ctcttttatc aagcaagcct tatatgggag gaaatcttgg gagtagaaaa aggaatccga
    13201 ttgttaggga ttactttgac gaatctggac ccgatgactt atgaaaacat cgtccttcca
    13261 ttatgggaaa atcaagaaat ataaaaacag ctttgagagt tgaaaccgga aatccgtttt
    13321 ctctcccaaa gctgttttta tatcaagtct tgctttttga tttgttcata ggtttctgtt
    13381 gcatccgtac atttttccca aatcacaact tgattggatt ctaaagattt ttggacgtca
    13441 atgatccgct ggttgctact tccgcgaaat tgtagtttta ggtcacgttt gcttaattca
    13501 aagcgtccgt caacaaggat atcgatttgc gaaaggagtt ctaatttatc ttctgaatct
    13561 tgtagcaatt catcaaaggt atagcctgtc catgaccaga tatcttttgt cgtaccgaac
    13621 tcttttcgga tacgtcgtac gacttgcaga caaacatctg tattcaaaaa aggttcgcca
    13681 ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat ttgttcttct
    13741 aattcaggtg tgaatggttt gccatatcgg aaattttgtg cagctttgtt gaagcagcct
    13801 tcgcaggcaa ataagcagcc actaacgtaa agactgttgc ggacaccttc accatcgaca
    13861 aaattaaacg gtttgtagtc agcaatatag ttttggctgt agtcttttgc taaccattct
    13921 tttggttttg gatttctcat aaaaaaactt ctttcattta aggttcaaag atatagctat
    13981 tgaagataaa atctactcga ccacataaat aagaacaaac tgacctaaat ccattgttca
    14041 agaaagcttg ttcttatttt gatagagggc gttcaaagat acaaagccat cgaaagaggg
    14101 tggtgaaaaa agacttgtca tagcctctta ttctgaataa acggtgttca aaagcttgca
    14161 tctgcagtaa taagcccaac taaacgaaaa atatgaggaa ctatttttgt ttagctattc
    14221 ttattacttg atgcaggaca gctttttcac aacctcattt catgtgtttc acacgtgagg
    14281 aaatttcttt atggcggcca tgtaccatcg gacgagcttg cggatttcct aaatagccac
    14341 aagtacgttt gactacatca catgtttttg gatcatgatt tccacattct ggacattcga
    14401 accctcgttc tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa tgatcgattg
    14461 gcgtattcgt accaagatac ccgattttgt catatgcaaa atcccataca gcttctaaag
    14521 ctttcggatt ttgttggagc atagggtatt cacagtagtg gatgaatcca cctgaacaat
    14581 attttggata atccttctca aattccaatt tttcaaatgg cgttgggttt ttacgcacat
    14641 catagtggaa actgtttgta taatattctt tatctgtgat attttcgatg atgccgaatt
    14701 tttctgtatc caaacggcag aaacggtcag ttagactttc acttggagtt gaatagacac
    14761 tgaaatgata accatattca tccccccatg catctgcatt agcttttaat tctttgacga
    14821 tatcaagtgt gaaagctttt gcttctgggt ttgtttccca ttcgccaccg aagaaagctg
    14881 aagcaacttc atacaatcca atgtagccta acgatacagt agctcgacgg tttttgaata
    14941 attcattgac agaatcggta cgtgaaagac gtttcccgaa agcaccatac atatataaaa
    15001 ttggcgcatt tgctggaatg gcttctttac aacgttctac acgatagaga agcgcatctt
    15061 tcatgatttc taagcggtca gacagtaact gccagaattt attctgatct ccttttgatt
    15121 cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa attcattcgt ccgacattta
    15181 cttcttgacc attttcgtct ttccaccctt gcaagaatga acgacagccc ataggtactt
    15241 tgaaacttcc agttaattca acgattttat cgtaattcag aatatcagga tacattcttt
    15301 tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt aggatcttca gggtttaagt
    15361 tcacaccttt tttcaaagtg aagatcagtt tagggaagat agctgtccgc ttttcactcc
    15421 ctaatccatt gattcgaatc tgtaaaattg ctttttgaat ttctctttca aaccagtttt
    15481 ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc atttgaagta aataaggtat
    15541 tgatttcata ttccaaactt tgcatagcat cgaagatatc ttttttggtt tttgcttttg
    15601 catattcttt ttggcgttct ggcgaatcga tccattcttc cgcgtctttt agatgttttt
    15661 tatagttcaa ttctgcaaaa ggagcaagca attcatctgt tcggtcggca gaacagccac
    15721 catattgact tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg gcggtctgga
    15781 tagattttgg cgattcgact tcggcattgc cgattttgaa cccattgttc agcatgcctt
    15841 tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg atgataatcc aaatcatggt
    15901 aatggatatc gcctttttga tgcgcatttg ctatatgtgg tggcaacatt ttcagtccga
    15961 ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca ttactgtctt
    16021 tatttgcgtt ttcgttcacg acagtttggt ccttgttgat tagcttttca atcgaaaagt
    16081 tgatatctgt tgctttactg cgagcaaaat ctcgtttggt acgataattg atgtactctt
    16141 ctgctaattc atattcgttt ttttctagta atatatgttc cacgatgttt tgaatctcat
    16201 agattttgac attttcagca aaccggcttt tgatttcttg gacaaccaag tctgcaatca
    16261 tttgaatttt ttcataagta agtggtgata atgagccgtg aattttcttt tctgctttga
    16321 tcagtgcttc attgatcttt tgttcatcga aatcaacatg gcggccatcg cgtttgatga
    16381 ctttcatctg ggatgtctct ggctgctgag taacgacagt tttttcttca taatttactt
    16441 tcattatctt caactcctgc taaattgttc ttgtctatca taaaacaaaa tcggggaagg
    16501 agcaactaaa tatcggctat atatagtatt caatgtgctg tataattgga tttctatact
    16561 atatgtagtg tatattttat tgtgaaaaaa ggtgaaaact tgtagctggt ggatttgttt
    16621 tttgcatatt taattttgct gaataaaata attgaataaa attttttatt cagtccaagt
    16681 aaaagtgaca aaaacataag agcaaattat tcgtagatat gctactatgc aaataaatag
    16741 aaatgaaaaa tgaaagagga ggagaagaat tggaaaagat gatcgaagtg aaacatctaa
    16801 gtaaaagcta cggcagcaaa ctttctcccg tcaaagcctt agataatatc tcattcacag
    16861 tagaagaagg cgagtttgtt gggattatgg gcccaagtgg tgcaggaaag acaacgctga
    16921 tgaatatctt gtccacgatc aatttgccta cgatgggcag tgtctcgatc caaggaaaag
    16981 aaatcacaaa aatgaaaaac agtgagctca gtgactttcg tcgaaaaaaa ctaggattca
    17041 tttttcaaga gttcaatttg atcgataccc taaatgcaaa agacaatatc ctattgcccc
    17101 tagcagttga acggatgact aaagaggaaa tggaaaagag agtaagacat gtagctcaat
    17161 tgttgaatat agaagaactg ctcaaacggt atcccgatga actttctgtc ggacagagac
    17221 aacgaattgc agctgctaga gcacttgttg ttcagccaca agtgattttt gcagatgaac
    17281 caactggctc tttggactcg aagtcggcta cagaattgtt gaactacttg aagaccatga
    17341 atcaaaaaga aaaagcaacg atactgttag tcacccatga tccatataca gcaagctact
    17401 gcgatagaat tttatttatt aaagatggtg tgattttttc tgaagttgtc agaagaggga
    17461 caagaagaga gttctttgag aaagtaatag acatgcaggc gacgattggc ggaggaggaa
    17521 aaatgaatgc tgtataaact agccgttaaa agtttttttc ggcagtcacg aggctatctt
    17581 gtttattttt tcagtttgac tttatccgta atgatctatt attccttcag cgcaatgacc
    17641 tacgatcaat ctttagtcag aagagcaagt caagatactt cgatagatgg tggattgagt
    17701 tttggtggat ttatcattac tgttgttttg ctttttttcg tattcagtgc caaccgattt
    17761 ttcttgaata gaaggcaaaa ggaaataggc atctatcagc tttttggtat gaataaactg
    17821 cagatatcag gtatatatgt gttagaaatc atgataattg gattgttcgc ctgtatttct
    17881 gggatattac tcggtattat tttttccaaa ctcttttcaa tgattttggt tcgaatgatg
    17941 gatatggatc ttactagtcc attttttatc tccattccat cagtagtaga tactttattt
    18001 gcttttttca tcatcctttt agcagtatct gtatattcaa tatggaaaat atggcgctat
    18061 ccaatgattc gaagttttgg agaaaaagaa caagcagata gttcaacaat gcgcttcagg
    18121 acaaggcacc gattactcgc agtcatcggg ttactacttt taacgagcgg atattttgga
    18181 gctagtcatt ttagggaaat cacttcatgg atgatcagtt atcattatct tgatacatta
    18241 gttttctttt ttcctttttt aatttttttc gtttgtgtgg tcggtactta tttgtttttc
    18301 tgttatacat ttcggttatt tttgatcata ttgagtaaat ggaagattta ttactatggt
    18361 atcaattttt tgataatggg taacacgcaa gttcatttgc tcaaaagttg gcgtacaaat
    18421 tctctgatta cagtcattct cggaatttct ttaatgacaa ttggcggcac ggctagtatc
    18481 tctactattt tatcctacca ggagaaagtt tcctccccga tggactatca gtttgatgtt
    18541 gagacagaga aaaaaatcaa gccgattcta gcagaggaag ggcagaagat aactaaagaa
    18601 atcaccttgc attataaagt cattgggagt atctatagtc aaaatctcga agaacctatg
    18661 tttcaaactg ctaatctgat cacggaaaca caatatcaag ccttcagaaa agttaatcca
    18721 gatttgccga acatttcttt gaaatcacct ggtcatactg ttttgcttga tcaaatgcaa
    18781 actctttttc ggaatatttc attatatgga tctagtattg gactgccaga taatcaagaa
    18841 ttgaagattc aacaaatgct tccaagtgta ttaggtgacg aaacaatggt atatctaagt
    18901 cctgtactga ttgtagatga taccgttttt gaaaaagcaa aaggtgtgga ttatcaagta
    18961 gtcaatgtag atgtatcagg tggaaataat gaaaaaatag atgaacgact gagcaatgac
    19021 ctgtcgataa agtggctcaa tgctgtttat tattcttatg atatcgtaaa tggaagtcta
    19081 caaggagaaa ttagcacaaa gaaacttccg gatgaccaaa tgaaggaagc acaatttagc
    19141 caagaaagct ctcgtttgaa tttctcttcc agctattcga aattgcgtgc agttaatcgg
    19201 cgaatcggta tcaatatttt tgtcacacta tttgtcggga tgatcgtgat tattgctact
    19261 ggtagtattt tgacagtccg gcaattagca gaagcagagg aagaaaaaga aaattatcac
    19321 ctattgacta agctggggat tccacaaaga aggatcagac ggatgatctt cgagcaaaac
    19381 gcacttacct tctttccacc catgatatta gggatcactc acgcagtatt tgcaatcaat
    19441 gtcttcactc agtatataaa aactgccgat tactggttgg cttatcttgt gagtggctta
    19501 ttgatcctta tctatctttt gctttatgtc attacgagtc agttatattg tcggattatc
    19561 gaggaatgat atacggaata agacaagata ataaatagaa ctaaattttg aataccattt
    19621 atttggaata agaggttgtg acacgatttt tgtcacaacc tctttatttt ttatattttt
    19681 taaaagtacg agaaattata aagaaaaaag cagtgatttg atgtgctatt tttataaaag
    19741 ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa gctagacaca tgtttctttt
    19801 tgtcaaaatt agttacactt tgtgtgaggt tatatgagga gggaatccaa tgaacaaatg
    19861 gcgttctaat ccaaaattga attattgggg aaaattcatc attcgaacaa ttttttatag
    19921 tgcagtactg ctgttcctga tttatctgta tcactataaa aatattcaag gaggaacttt
    19981 tatctataac gaattttagg aggaagagta tggaaatcaa aacgattatt gaagcaatcg
    20041 atgattgggg aataaatgaa cctgatcgag ttgcttatca ggcggatgag acgcatactt
    20101 ttggtgaatt gaaacaagca tcagacgcac ttgcttatta tttagaaaaa aaggtagaag
    20161 gcgatggacc aattgtcgtt tttggcaatt tggagtttga aatgattgtc tcatttctag
    20221 gggttgtcaa atctggccat gcctatttgc cgattgaaga gcatacaccc aaggagcgta
    20281 tcctatcgat ctttcgggtg gctaaaccat ctatggtcat cagtatcggg gactggatag
    20341 aagatattcc ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc aagaagatac
    20401 cagtaggatt ttatcccaga aatagcgtaa aaggtacaga aaatttttat attattttta
    20461 cttctggtac tactggagaa ccaaaaggcg tacaaatcag tcatgataat ctagtcagct
    20521 ttgttcaatg gacgatggaa gactttggga tcaagcctgg tatgcatttc ttagcccaag
    20581 cgccattttc ttttgattta tcggtatttt ctatttatcc tgcattagtt tctggcggaa
    20641 tgctgaagcc gttaaataaa gcagttgttc aagactttcg tcaattattc gcgacattgc
    20701 cagccttgaa gttgaatgtg tgggtttcta ctccatcatt tatggatatt tgcttgatgg
    20761 aacctacatt caatggagaa aatgtgccgg aattggacat gtttttattt tgcggagaag
    20821 aattgacgaa aaaaactgca gaaagtctac ttgatcgatt cccaaatgca cggatatata
    20881 atacttacgg tccaacagaa gcaactgtag ctatttcaag tattcagatt gaccagcatg
    20941 tactagatag ttatgaccga ctaccgattg gctacgtaaa aaaagacacg aaagtttcta
    21001 ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt attgcaggac
    21061 caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg ttcttttgtc
    21121 aaaatgactt agcaagttac catacagggg atgcaggtag actagatgaa tatgggcttt
    21181 tattttatga aggacgtatg gatcagcagg tcaaattaca tggataccgg attgaactcg
    21241 gggatatcga acattatctg ctccaagaca accgaatcaa acaagctgtc gtcgtaccaa
    21301 aataccaagg aacaaaagta cagcagctgg ttgcttttgt cgtattagaa aacaaaacga
    21361 aacagccgga ttttcagttg actaaatcta tcaaagaaca attactagaa gttgtaatgg
    21421 attatatgat tcctcagaag ttccattatg tcgatctgct gcctcagaca gttaatggaa
    21481 aaatcgatcg aaaaaaatta acagctgagg tgaatcctga atgatgttac ctcatatgat
    21541 cccttatgca gagccgtttt atttttttct tttagccatt gcgcttgtac cgatcgttct
    21601 atctttattg atatgggaaa aaagattacc agtttatcaa tcagcagtga cacttttctt
    21661 tttatttgtc agctttggag gtaattattg gcaacaggga gcggcgctga ttgggtatgt
    21721 tttgtggcag acgatacttg tatggggcta ttttgtttat cggcaaagga aaaatcagac
    21781 aggatggttt tttgccgccg ttattttagc gataattcca ctgtttttaa cgaaagtgac
    21841 tccttttttt gaacatggaa aagcatcgtt acttggtttt ttaggaatct cttatttaac
    21901 ctttaaatct gtacaaatga tcatggaaat aagagatggc atgatcaaaa catataagcc
    21961 atttcgttat atccagtttc tgctgttctt tccaacaatc tcttctggac caatcgatcg
    22021 ctataggaga tttgaaaaag atcagctcca tccgcctgtt cgtgataaat atgttgatct
    22081 tttgggaaaa ggcgtccaaa atatttttgt cggtttttta tataaattca ttatcggtta
    22141 ttattttggt caagtactgc ttcctgtagt gggacgcatt gccttacagc atggtggtat
    22201 ttcatgggca ttagtaggtt atatgtacgt gtacagtatg tatttgtttt ttgattttgc
    22261 tggctatagc ttatttgcag tcggtacaag ctacatgatg ggatatcaga caccaatcaa
    22321 tttcaataaa ccttttttat catggaatat caaagaattc tggaatcgtt ggcatatgac
    22381 gctgtccttt tggttccgtg attatgtata tatgcgattg atgttcttca tgatgaagaa
    22441 aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt tgtttcttct
    22501 aatgggtata tggcatggat tgacgtggta ctacatcgta tatggactgt atcatgcttg
    22561 tctgatctgt ctgacagatg catggatccg atttaagaag aagcataaag agcagttgcc
    22621 ttctaacaaa tttacgcatg gcttagccgt atttttgaca tttcaagcag tatgttttag
    22681 cttcctgatt ttttcaggat ttttagataa actttttttt agctaacgaa tagtgacata
    22741 ttaatttata taacaaagga gaaaaggaaa atggaagaac aaattttatc aatcttagaa
    22801 gaaataacag gaacagatga agtaagaaaa aatcgtgata ttgatctatt tgatgaaggg
    22861 ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc tagacggaga gctggatatc
    22921 caagtccctg tatctgaatt cgagcgagag gattggagca caccgaataa aatcattcaa
    22981 caagcaactg cattgaaggg ttgagtatga aaaagaaact ttttgctatt ttcggcccgg
    23041 ttgttttagc tgttggctta ctgtttgtgt tttttacctc ctcctatcaa gtagatatct
    23101 ctaatccaga aacgattaga cgtgcctctt cttccatgtc agtgaacgtg ttgaaaggag
    23161 atgcgataaa aaattatgca ttatctgaaa aacaatatat tccttttttt ggctcaagtg
    23221 aattaagcag aatcagtcca tttcatccgt ctgttctggc agaaaaatac cagcgaaact
    23281 atcgtccatt tttgctggga gcacccggga cacaatcgtt aagccaatat atgatgatgc
    23341 gttctgcagg agatgctatg aaaaacaaga aagtcgtctt tattatttct cctcagtggt
    23401 tcgttaaaaa tggtgtaaaa actgattact ttaatacgta ttattctgaa cttcaaacat
    23461 acgactggtt gttttcaatg aagaaagtaa cgcctgctga tcgctattta gctagaagat
    23521 tattaacttt ctcaaaagta aaagagaatg atacgttgac ggcgattctg caaacgatca
    23581 aaaaagggaa gctaccttta cctgaatctt tgaatcagct gcgcagccaa tggaacatgc
    23641 tgaagagaga ggatgaagta ttcagcaata ttgggttgaa agatcgacag caaaaaatcg
    23701 atcatgagtc aaaacgatta ccaaaacaat accaagaaac agaattatcc attttagcaa
    23761 atcaaattgg agaacgagaa acgacgaata atccattcgg gttgaaaaac gatttttata
    23821 cgcatcgtat ccgagcccac gagccagagc tcaaacagtc acaaaaaaat tgggattatc
    23881 gtttttcgcc agaattttct gattttcagc tggtcttgga tcaattagca aaaaaccata
    23941 acgaagtatt gttcatcatc ccaccggtta acgagaaatg gagcgattat accggactat
    24001 ctcaagaaat gttacaagga tttgccaaaa agatcaaatt tcaattaaat agccaaggat
    24061 tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa ctattttatg gaagacacga
    24121 tccatctagg ctggaaaggc tggttggctg cagatcaaca gatcagaccg tttttagaag
    24181 aaaatcatat tactgctagt aagtatcatc ttgacgatgc tttttttagc aaatcttggc
    24241 aacatcaaat accagataaa ctacaattaa aataaagaaa acaggcgagt gttgctcgct
    24301 tgttttcttt atttttgaat attctgaata aaagataaaa ataccaagtt tgcctgaaca
    24361 aaaatcataa gcaatggtat aatcacaata tgaatataga aatgggggaa aacatttgga
    24421 tacaaaagaa gaacagctac ttattgattt aacttctgca aaaggagtac ctggaaatga
    24481 agaagaggta agagaagtat tcagagaata tgccaaacca ttcgctgatg atatttttta
    24541 tgacggttta ggcagtgtga ttgccaaaca tggtgctggc ggaggcccca aggtctttat
    24601 ttctggacat atggacgaag ttggttttat ggtaacaaaa ataaccgaaa aaggattctt
    24661 ggaattccaa actcttggag gttggtgggg gcaagtcatg cttgctcagc aagttgaaat
    24721 caaaacacgt gaagggaaaa tcgtccacgg tgtgattggt tccaaaccac cacatgtttt
    24781 aacacctcaa gtaagaaata aaccttatga aataaaggat atgttcattg atattggcgc
    24841 atcaagccaa gaagaagcca aagagtgggg catacgtcct ggagatatgg tcactccata
    24901 tattgaatac aaacgaatga atggcagtaa atatttactg gcaaaagcat gggataatcg
    24961 tatcgggaca gctgtttctc tgcgagtatt agagaatcta tctaaagaag cacacccaaa
    25021 tgttttattc gcaggaagcg atgtgcaaga agaagtaggc ttgcgaggtg ctcgaacaag
    25081 tacacacctt gtaaatcccg atattgcttt tgctttagat actggtacag caggagacac
    25141 tccaggaatg acaccaaaag aagcagattc gattttagga aaaggcccac agattttgat
    25201 ttttgatgca tctatgatcc cgcataaaaa attattgaat tttgttattg atgtagcaga
    25261 ggaaatggat attccattcc aatatacagt gatttctggc ggaggaactg atgcaggaca
    25321 aatgcatttg acaagagatg gtgttccgtc tgtagccatt accgttcctg ttcgttatct
    25381 gcattcccat acatcagtga ttcatgaaga tgattatttg aatacagtga aactagtcac
    25441 agaattagtg aaacggcttg atagtgaaac agttgaaaaa attcggagtt atgaataatg
    25501 aataatagaa tacctatttc tgaatatcgg aaaaaagcac gagtttcttt ggaaaatcaa
    25561 tggggaatca atacttggct tgttttctta tctataatat ttacgagcgt catccagaac
    25621 ttttttgacg gtttcagtcc ttttccggaa gaaagcatgc agcagcactt attgagtttt
    25681 ctagtcgata atttactttt attcgcattc aactatgcat tgttttacat tgcgctagta
    25741 gttgtacggg gtggcagacc aaaaagtaac cttttatttt cagttttcca aagaaagtat
    25801 tttggttcga tacttggcat caatttgatc aattcagtca tcaactggct actcaacagt
    25861 ttaatctttc tgcccatgtt tttaatcagc ggagtgagta cttatatgca gctgcttttc
    25921 agttcaaatg cggtacctat gggtgtcatg ccagaagatt ttcttgatat cagctttgtt
    25981 ttatcgatga tagggatgat ttttgtttca gcactcatca tgagtattgt tggcggttta
    26041 ttccaatttg ctgcatggac aaagttcgac tatcctgatt tgtcggttat tcaatgtttg
    26101 aaatatggct ggtttttatt gaaagataga attggtacgt atattttgtt gcagctgtct
    26161 tttatcggct ggtatttgct aggagcaatc gctttgttca tcggtctttt gtgggtcatt
    26221 gtttatgtga atgtgtcagt tgcagaattt tatgaacaag cacggattga aaaaggaggt
    26281 ccagacgcct attttactta gcatgtaagc agtttattca tcaaaaaaat aacttttgtg
    26341 gtaggataaa tctttgaggg ggaatgtacg atgaaattaa ctgttttagg atgcttagga
    26401 gcgtatcctt ataaaaatca agggacaaca ggttatttgc ttcaatctga cgggttcaat
    26461 ttgttgatcg atgcaggtag tgctacgctg atcaaattac aagaacacct tgatccactt
    26521 gatctagatg ctgtgatcct tagccattat catcatgatc atattgctga ccttggtgta
    26581 ttacagtatt actggcaact tcatcctaag agaaatccgg agacattgct ttcaatttat
    26641 ggacacacaa aagattccat tcactttgaa gaattgacgt tggaaaatgt ctcaaaagga
    26701 catccttatt ttgaagctga agaattaaaa attggtcctt ttctgattac ttttatggaa
    26761 acaatccatc cagtagtatg ttacgccatg cgtatcgtag aagaaacaac ggggaaagta
    26821 ttcgtattta caggtgattc aggttatttg gaaagtttcg ctgattttgc gaaagatgct
    26881 gatttgtttc tagcagatac ctatcttttt gaaggaaatg aacatcatca tgcccatttc
    26941 acttcaaaag aagctggaga acttgcgaaa tcagctaacg taaagaaact tgtacttaca
    27001 cacctacctc agtttggtga tttagaacaa ttgaaaaatg aagcgcaaaa ggcagcaggc
    27061 aaagggattg atatagaact tgctgcagtt gacaaagtgt ttaacattta ggaggaagaa
    27121 catgattttt gtaccaaatg aaaaccacga cccaagaatc aatttagcaa ttgaacaata
    27181 tttgctgcaa gaaatgcctt tagatgaacc tattttgctc ttttatatca atgaaccttc
    27241 aatcatcatc ggcagaaacc aaaatacgct tgaagaaatc aatcgagatt atgtggaaga
    27301 aaatggcatt catgttgtcc gtcgcttaag tggaggcgga gctgtctacc atgattttgg
    27361 aaacctgaac tttagtttta tcatgcctga tgatggggat tcattccgtg actttgcgaa
    27421 agtaacacag ccaatcatcc aagcacttca tgaattgggt gttgctgggg cggaattgaa
    27481 aggccgaaat gatctagtca ttgatggcat gaaattttct ggcaacgcga tgtatgctac
    27541 caacggtcgg atgtttgctc atggaacaat tatgttcgac tctgatatca atgaagtagt
    27601 caatgcatta aaagtaaaaa aagacaaaat cgaatcaaaa ggaatcaaat cgatccgttc
    27661 tcgtgtaacg aatatcaaac catttcttcc agaagaaaag caaaaaatga caacagaaga
    27721 atttcgtcaa gaaatcttgt tgaaaatttt cggcgtagag tcagtagaag aagtaaaaac
    27781 gtacgaattg actgatgaag attggaaaaa aatcaatcga atctctgagg aatattatcg
    27841 aaattgggat tggaattacg ggaagtctcc cgatttcaat ttctctcgcc aaaaacgttt
    27901 ttctatcggt tcgattgaag tccatttgaa tgtctcagaa ggaatgatca aagaagcaaa
    27961 aatctttggc gatttcttcg gactaggaga tatctctgac gtggaaaaaa aattggttgg
    28021 acagaaatat gataaagcat ctctatcaaa aatagtcagc caaattgatg tgaagaaata
    28081 ttttggtgcg atagaaccag aagaattgct taccttactt tattgaaaat aaaaaatagc
    28141 ggaatccaca tatatggatt ccgctatttt ttatacagga ttcgggtaat ttcgagagag
    28201 ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc gttagtgttc ctgtgtaacg
    28261 atccgctacg tctttcatca tcattaagtc aaaacgttcc tgatcatcta attcttctgt
    28321 gatgcctcga ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac aaatgacgaa
    28381 atgttcctct tgttcgtgta tcgacaaata aatttttcga tacagtggat tttcgatggc
    28441 atgagtttct ctaaaggaat gctctaaagc gtggatcaaa agttgagcca gatcgtcatc
    28501 gttgatagga agttcttcgg gaagatctgc ttggaaggtt atctgaatat tccgtttggc
    28561 agctaacttc tggtaacgcg ctaagataag atttgtcacc tggtggttgc aaaaagaaca
    28621 cagcatttcg aaattttttg cttcttctaa tatcttttcc acatagtttt tagcttttgt
    28681 ttggtccgta tgcattaaat cttgcagatt ctccatctct tggacaaatt ccaggcgaag
    28741 tgtatttact tcttggatat gctgtcgaag ctgctcataa tgttgctcca acaaatcatt
    28801 tttgattttt aagaaatcta cttgtctccg atctttgtcg gtatttaaga tgtgatcgat
    28861 tagattgtag ctgataataa cgattagaat gataatatat aaaatcgtag accaattgat
    28921 cgttgtttga tagataccta atgccgtttg gatgtaaatc aagcaatgga taatggctac
    28981 caagacgatc caaggtgtac aagctacata aaaacggtta ttcttatatg cttcgccaat
    29041 gcatgcaagt gaagtgacta aagtgcttat aacactagca atattagcaa tcggcattaa
    29101 ttcaggaagt tctgcaggag taaaagttgc ataaagcaga acgccgataa aaatcaaaag
    29161 ttggaacgta caaaaaattc cgtaccattt tttatagtaa aacattcggc tccaatagta
    29221 gcagatgatg aaagctgacg ttaagataat aaataaatga gataaaaaag aatgaacgaa
    29281 tggttcaaaa agaataccac ttaaaatatc ttctgataca gcttcaaatc ctaatgccaa
    29341 agcaaagcaa cttaagagaa taagtgagta ttcaagacgt ttttgtttat acatcaagta
    29401 gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg tcaatacttg
    29461 cggcactgat tgtgaataaa taaaactaat caatggtcca gttgttccta gataaacttt
    29521 cggtggcagt ccagcatagt tctgataagg agatgagact gtaagggtca atttttttcc
    29581 gatataattt gctggtaaat ctatcacagc caatgagagt ccgggattgc tttcttcctg
    29641 ataatctttc cgttggtata aaatcttttc gcctagatat acagtaatcc attgatggtt
    29701 tcctttgatt agtaaatttg cttcgtccaa cggtttttca agtgtcgttt ccataatgaa
    29761 tgtttcgtct ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa acttaaaatc
    29821 aggatcatct tttgtataga aaacccacga atctttta
//
LOCUS       DAFCLG010000020        29341 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFCLG010000020 DAFCLG010000000
VERSION     DAFCLG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.020"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="LND56_000885"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="LND56_000885"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK6742827.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="LND56_000886"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="LND56_000886"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK6742828.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="LND56_000887"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="LND56_000887"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK6742829.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="LND56_000888"
     CDS             complement(3376..4683)
                     /locus_tag="LND56_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBK6742830.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="LND56_000889"
     CDS             complement(4686..5267)
                     /locus_tag="LND56_000889"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK6742831.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="LND56_000890"
     CDS             complement(5839..7131)
                     /locus_tag="LND56_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6742832.1"
                     /translation="MKKKYVQVGLGGRARFFYETIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="LND56_000891"
     CDS             complement(7128..7751)
                     /locus_tag="LND56_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742833.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="LND56_000892"
     CDS             complement(7754..8590)
                     /locus_tag="LND56_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6742834.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="LND56_000893"
     CDS             complement(8607..9494)
                     /locus_tag="LND56_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6742835.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="LND56_000894"
     CDS             complement(9510..10793)
                     /locus_tag="LND56_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6742836.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="LND56_000895"
     CDS             complement(11476..12159)
                     /locus_tag="LND56_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK6742837.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="LND56_000896"
     CDS             complement(12177..12794)
                     /locus_tag="LND56_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6742838.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="LND56_000897"
     CDS             complement(12804..13628)
                     /locus_tag="LND56_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK6742839.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="LND56_000898"
     CDS             complement(13621..14181)
                     /locus_tag="LND56_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742840.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="LND56_000899"
     CDS             14355..15311
                     /locus_tag="LND56_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6742841.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="LND56_000900"
     CDS             complement(15616..16572)
                     /locus_tag="LND56_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBK6742842.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="LND56_000901"
     CDS             16676..17278
                     /locus_tag="LND56_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6742843.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="LND56_000902"
     CDS             complement(17366..18865)
                     /locus_tag="LND56_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK6742844.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="LND56_000903"
     CDS             complement(18881..19405)
                     /locus_tag="LND56_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK6742845.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="LND56_000904"
     CDS             19703..20452
                     /locus_tag="LND56_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6742846.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="LND56_000905"
     CDS             20443..21228
                     /locus_tag="LND56_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBK6742847.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="LND56_000906"
     CDS             21197..21913
                     /locus_tag="LND56_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6742848.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23049)
                     /locus_tag="LND56_000907"
     CDS             complement(22015..23049)
                     /locus_tag="LND56_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBK6742849.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23053..24111)
                     /locus_tag="LND56_000908"
     CDS             complement(23053..24111)
                     /locus_tag="LND56_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK6742850.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="LND56_000909"
     CDS             complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="LND56_000909"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK6742851.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25556..26392)
                     /locus_tag="LND56_000910"
     CDS             complement(25556..26392)
                     /locus_tag="LND56_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6742852.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26576..27793)
                     /locus_tag="LND56_000911"
     CDS             complement(26576..27793)
                     /locus_tag="LND56_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6742853.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(27942..28121)
                     /locus_tag="LND56_000912"
     CDS             complement(27942..28121)
                     /locus_tag="LND56_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742854.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            28176..28259
                     /locus_tag="LND56_000913"
     tRNA            28176..28259
                     /locus_tag="LND56_000913"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28210..28212,aa:Leu,seq:gag)
     gene            complement(28499..>29341)
                     /locus_tag="LND56_000914"
     CDS             complement(28499..>29341)
                     /locus_tag="LND56_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742855.1"
                     /translation="NQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFS
                     LTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFL
                     LTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTE
                     LGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMG
                     FSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIIT
                     VINR"
BASE COUNT         8758 a         6263 c         5035 g         9285 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgtctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    24181 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    24241 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
    24301 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
    24361 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
    24421 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
    24481 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
    24541 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
    24601 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
    24661 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
    24721 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
    24781 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
    24841 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
    24901 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
    24961 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
    25021 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
    25081 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
    25141 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
    25201 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
    25261 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
    25321 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
    25381 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
    25441 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
    25501 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
    25561 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
    25621 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
    25681 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
    25741 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
    25801 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
    25861 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
    25921 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
    25981 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
    26041 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
    26101 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
    26161 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
    26221 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
    26281 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
    26341 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
    26401 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
    26461 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
    26521 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
    26581 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
    26641 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
    26701 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
    26761 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
    26821 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
    26881 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
    26941 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
    27001 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
    27061 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
    27121 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
    27181 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
    27241 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
    27301 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
    27361 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
    27421 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
    27481 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
    27541 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
    27601 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
    27661 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
    27721 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
    27781 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
    27841 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
    27901 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
    27961 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
    28021 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
    28081 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
    28141 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
    28201 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
    28261 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
    28321 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
    28381 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
    28441 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
    28501 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
    28561 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
    28621 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
    28681 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
    28741 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
    28801 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
    28861 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
    28921 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
    28981 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
    29041 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
    29101 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
    29161 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
    29221 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
    29281 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
    29341 t
//
LOCUS       DAFCLG010000021        29108 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFCLG010000021 DAFCLG010000000
VERSION     DAFCLG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.021"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1031)
                     /locus_tag="LND56_000915"
     CDS             complement(<1..1031)
                     /locus_tag="LND56_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6742856.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="LND56_000916"
     CDS             complement(1113..1802)
                     /locus_tag="LND56_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6742857.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="LND56_000917"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="LND56_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK6742858.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="LND56_000918"
     CDS             complement(2329..2832)
                     /locus_tag="LND56_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK6742859.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="LND56_000919"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="LND56_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK6742860.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="LND56_000920"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="LND56_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK6742861.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="LND56_000921"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="LND56_000921"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBK6742862.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="LND56_000922"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="LND56_000922"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBK6742863.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="LND56_000923"
     CDS             complement(6444..7463)
                     /locus_tag="LND56_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6742864.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="LND56_000924"
     CDS             complement(7636..8541)
                     /locus_tag="LND56_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6742865.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="LND56_000925"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="LND56_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK6742866.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="LND56_000926"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="LND56_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK6742867.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="LND56_000927"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="LND56_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6742868.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="LND56_000928"
     CDS             9754..10398
                     /locus_tag="LND56_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6742869.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="LND56_000929"
     CDS             complement(10454..10957)
                     /locus_tag="LND56_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6742870.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="LND56_000930"
     CDS             11554..12519
                     /locus_tag="LND56_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6742871.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="LND56_000931"
     CDS             complement(12618..13790)
                     /locus_tag="LND56_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742872.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="LND56_000932"
     CDS             complement(13777..14922)
                     /locus_tag="LND56_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742873.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="LND56_000933"
     CDS             complement(14936..15868)
                     /locus_tag="LND56_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742874.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="LND56_000934"
     CDS             15985..17064
                     /locus_tag="LND56_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6742875.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="LND56_000935"
     CDS             17061..17690
                     /locus_tag="LND56_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6742876.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="LND56_000936"
     CDS             complement(17800..18027)
                     /locus_tag="LND56_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6742877.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="LND56_000937"
     CDS             complement(18130..20718)
                     /locus_tag="LND56_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBK6742878.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="LND56_000938"
     CDS             complement(20738..21397)
                     /locus_tag="LND56_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6742879.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="LND56_000939"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="LND56_000939"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK6742880.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="LND56_000940"
     CDS             complement(22170..23036)
                     /locus_tag="LND56_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6742881.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="LND56_000941"
     CDS             23145..23786
                     /locus_tag="LND56_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK6742882.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="LND56_000942"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="LND56_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBK6742883.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="LND56_000943"
     CDS             complement(25227..26126)
                     /locus_tag="LND56_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6742884.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="LND56_000944"
     CDS             complement(26128..26925)
                     /locus_tag="LND56_000944"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBK6742885.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="LND56_000945"
     CDS             complement(26903..27607)
                     /locus_tag="LND56_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBK6742886.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="LND56_000946"
     CDS             27738..28313
                     /locus_tag="LND56_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBK6742887.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="LND56_000947"
     CDS             28431..29108
                     /locus_tag="LND56_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6742888.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6169 c         5232 g         9143 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaa
//
LOCUS       DAFCLG010000022        28315 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFCLG010000022 DAFCLG010000000
VERSION     DAFCLG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.022"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            197..1162
                     /locus_tag="LND56_000948"
     CDS             197..1162
                     /locus_tag="LND56_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6742889.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1219..1449)
                     /locus_tag="LND56_000949"
     CDS             complement(1219..1449)
                     /locus_tag="LND56_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBK6742890.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1535..2422)
                     /locus_tag="LND56_000950"
     CDS             complement(1535..2422)
                     /locus_tag="LND56_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK6742891.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2511..3734)
                     /locus_tag="LND56_000951"
     CDS             complement(2511..3734)
                     /locus_tag="LND56_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6742892.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3751..4797)
                     /locus_tag="LND56_000952"
     CDS             complement(3751..4797)
                     /locus_tag="LND56_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6742893.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4961..6688)
                     /gene="ptsP"
                     /locus_tag="LND56_000953"
     CDS             complement(4961..6688)
                     /gene="ptsP"
                     /locus_tag="LND56_000953"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK6742894.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(6688..6954)
                     /locus_tag="LND56_000954"
     CDS             complement(6688..6954)
                     /locus_tag="LND56_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBK6742895.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7433..9667
                     /locus_tag="LND56_000955"
     CDS             7433..9667
                     /locus_tag="LND56_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6742896.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9815..10135
                     /locus_tag="LND56_000956"
     CDS             9815..10135
                     /locus_tag="LND56_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK6742897.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10340..10564)
                     /locus_tag="LND56_000957"
     CDS             complement(10340..10564)
                     /locus_tag="LND56_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBK6742898.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10762..12342)
                     /locus_tag="LND56_000958"
     CDS             complement(10762..12342)
                     /locus_tag="LND56_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK6742899.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12743..14119)
                     /locus_tag="LND56_000959"
     CDS             complement(12743..14119)
                     /locus_tag="LND56_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6742900.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14247..15365)
                     /locus_tag="LND56_000960"
     CDS             complement(14247..15365)
                     /locus_tag="LND56_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6742901.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15507..15941
                     /locus_tag="LND56_000961"
     CDS             15507..15941
                     /locus_tag="LND56_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742902.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(16019..16552)
                     /locus_tag="LND56_000962"
     CDS             complement(16019..16552)
                     /locus_tag="LND56_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBK6742903.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16646..17824)
                     /gene="mutY"
                     /locus_tag="LND56_000963"
     CDS             complement(16646..17824)
                     /gene="mutY"
                     /locus_tag="LND56_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK6742904.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17817..18614)
                     /gene="recX"
                     /locus_tag="LND56_000964"
     CDS             complement(17817..18614)
                     /gene="recX"
                     /locus_tag="LND56_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK6742905.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18721..20103
                     /gene="rlmD"
                     /locus_tag="LND56_000965"
     CDS             18721..20103
                     /gene="rlmD"
                     /locus_tag="LND56_000965"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6742906.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20277..20855)
                     /locus_tag="LND56_000966"
     CDS             complement(20277..20855)
                     /locus_tag="LND56_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBK6742907.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21177..21647
                     /locus_tag="LND56_000967"
     CDS             21177..21647
                     /locus_tag="LND56_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBK6742908.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21755..22981)
                     /locus_tag="LND56_000968"
     CDS             complement(21755..22981)
                     /locus_tag="LND56_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6742909.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23735..23935)
                     /locus_tag="LND56_000969"
     CDS             complement(23735..23935)
                     /locus_tag="LND56_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6742910.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(24069..24677)
                     /locus_tag="LND56_000970"
     CDS             complement(24069..24677)
                     /locus_tag="LND56_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBK6742911.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24762..25232)
                     /locus_tag="LND56_000971"
     CDS             complement(24762..25232)
                     /locus_tag="LND56_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBK6742912.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25280..26194)
                     /locus_tag="LND56_000972"
     CDS             complement(25280..26194)
                     /locus_tag="LND56_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6742913.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26340..27143
                     /locus_tag="LND56_000973"
     CDS             26340..27143
                     /locus_tag="LND56_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBK6742914.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27321..28268
                     /locus_tag="LND56_000974"
     CDS             27321..28268
                     /locus_tag="LND56_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK6742915.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         8546 a         5685 c         4757 g         9327 t
ORIGIN      
        1 tttttttaag attttactca tttaaaacaa ctgataaatc agcatttata atttctttta
       61 ttagaaaaat catctatctc acatattttg attatcaaag tattttctgt gaacagaact
      121 atagaattcc ctaaaacttg tccattattt caaaatttgt tatagtcttt ttattcaaag
      181 cgtaaaagga gatcctatgt taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt
      241 ctatcttatg aaagaccgag atggtaaggt catctatgtt ggtaaagcaa aacggctcaa
      301 aaatagagta agcagctatt ttcatcagaa taaacagcat tcaaaaaaag tattacgtat
      361 gattcatcat ataacagatt ttgatttcgt cgtagtagac acggaattag atgctttatt
      421 gctcgagtgt caattgatcc aacattaccg tcctttttat aaccgacaaa tgaattattt
      481 cagtaattat aattatgtac atattaccaa taagggattt gtattaacag atactcctac
      541 ggctcgaaca tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat
      601 ggaagaaact tatcaaatgc cttggttgtc tgaaattagt ttgttagctt tgcgagtcca
      661 gcttcctgac ctacaggaaa tgacttttga acagaaaaaa aaggaactcc aagggctgtt
      721 tcaaggccga aataaaaagc tactgactta cttgaaaaaa agacaacaac atttcatcta
      781 tcagttgaat ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt
      841 tattagacgg atccaggaac aaaagcagtt tttaagaaca ccatcgctca ccttcagcat
      901 gccattagca gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc
      961 agaaacaatg attacctctg gtgacgtttc tcctgacttc tattatgaaa agaaagaagc
     1021 tcatttatct ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat
     1081 cagttaccgc aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga
     1141 agctgaaaag caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa
     1201 tgtcaattat cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat
     1261 attatcaaat tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc
     1321 tttatcataa gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag
     1381 ttcattatct gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga
     1441 atgtgtcatc ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct
     1501 acttgcattg attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc
     1561 cttcaggcca ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg
     1621 ccaactcttc atatagtttc aatgcttggg tagttgctgg caatacaaga tccatttttt
     1681 ctgcttcttc taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg
     1741 tgtaatcttc ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc
     1801 cacctgaaag cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa
     1861 gcatttccgt cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat
     1921 gctgaccttt cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag
     1981 gcagtacttg atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct
     2041 ttgctcctaa gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg
     2101 ctgtttttgc aattttttct gccaggcttg gtgtacttgt agtcaaatca actaaaatat
     2161 gtcctgagat atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt
     2221 tggggtaacc taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat
     2281 ctgcccagat agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga
     2341 cagctacttc gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg
     2401 ttccgataaa accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta
     2461 tgctagtata cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta
     2521 aatgaattta attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc
     2581 tcttcttcat gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt
     2641 gtagatgaaa tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg
     2701 gaaatataat caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat
     2761 gaaggatgat cgaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg
     2821 gctgccatcg cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa
     2881 taatctgccg ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc
     2941 acaaattctt ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct
     3001 tttcctacga taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta
     3061 atcaaggctt ggatattttt ctcataagaa atcctgctta aagaaagaat catcaaatgg
     3121 ttttcctgaa gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa
     3181 cgtttgaatt tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca
     3241 tagctgcgta atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt
     3301 gcaaataatc tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata
     3361 taatgaaggt agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc
     3421 ttagctacca tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct
     3481 agttctagtt ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga
     3541 tccttgaaag aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc
     3601 gcattaggat cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt
     3661 tttaatgttt tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa
     3721 aagccgattt tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa
     3781 tttgctagag actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat
     3841 tttattgata cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc
     3901 ttgattggta atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca
     3961 gttcttgcta tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg
     4021 tttgcttgct aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa
     4081 gaatagatct gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa
     4141 caggacattt tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta
     4201 catgggaaca tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac
     4261 gaaatcaatg attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc
     4321 cggagacagt ttgaaatatt ctctgaactt ttgttctttc attggatcag ggtgatattt
     4381 ctctaagtcg atcccattgg aaatagcttg aatcggcaca cggataccat agctttccaa
     4441 caactgtttt gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac
     4501 cagatatttt tttacgatag gagaaagctg gttcgatccg ataaaggaat ttcggaaatc
     4561 ttcctctgta gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac
     4621 catattatga ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc
     4681 atctagtgta taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg
     4741 cccgattcca gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt
     4801 acgtccccct tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc
     4861 ctatttttgt ctattcttac tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc
     4921 gtagctcttt tacaagccac accagctttt gatacgttgt ttattttaca tattcatgaa
     4981 caagttcaac gacttcttcc atcgtatcgc attctttcaa tgcacgatca gcaagttcag
     5041 ccattttcgc tgtgtccaag cgtttcatta aactacgcgt tttaagaata gatgtcgcac
     5101 tcattgagaa ctcatctaga cccataccta ctagcaatgg aacagctgtt tgatctccag
     5161 ccatttctcc acacatacct gcccattttc cttcagcgtg tgctgcatca attacatttt
     5221 tgatcaaacg taaaattgat gggttatatg gttggtacaa gtaagaaacg cgttcgttca
     5281 tacggtctgc tgccattgtg tattgaatca aatcgttcgt acctacagag aagaaatcta
     5341 cttcttttgc aaacttgtct gctaagactg ctgctgctgg aatctcgatc atgatgccga
     5401 cttggatcgt atcagatact tccttgcctt cacttatcaa tttttgtttt tcttcttcaa
     5461 agatcgcttt tgctgcgcgg aattctttga gtgtagctac catagggaac atgatacgta
     5521 aattaccatg aacagatgca cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc
     5581 cttgttcaga taaactgata cgcaatgcac gatatcctaa gaatggattc atttcatgag
     5641 gtaattgcag atatggtagt tctttatctc caccgatgtc cattgtacga acaactacag
     5701 gttttccttc cataccttct aatacagctt tgtaagcttc atattggtca tcttctgttg
     5761 ggaagtctgg agaatccata tacaggaatt cagtacggta cagaccaact gcttctccac
     5821 cattattatg gacaccaact agatcttttg gtgttccgat gtttgcagct aattcaaaat
     5881 gcttgccgtc agctgtgaca gtttctgcat tttttaattt ttcccattct gctttttgtg
     5941 ctgcgtaatc tgcacctgct ttttcaaatt cagctttttg ttcatctgta ggatcgatga
     6001 ttacatcgcc ttcgatcccg ttgacagcca aaatatcgcc ttctttgact ttagcagtaa
     6061 tttcttttgt cccaacgatt gctggaattt ctaaagaacg tgccatgata gcagaatgtg
     6121 aagtacgtcc accaatgtct gttacaaacg cttttacaaa attgcgatct aattgtgctg
     6181 tatcacttgg agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc attgatggat
     6241 ttggtaaagt aacgcctaat aagtgcgcta aaatacgttt agcaacgtca cgaatatctg
     6301 cagcacgctc ttgcatataa gcattatctt ccattgcttc gaacataccg atatacatat
     6361 ccgttacttc ttttagcgct gattcagcat ttacgctgtt gtctttaata ttttgcttga
     6421 tttgacctac catttctgga tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt
     6481 ctgcttcacc taagctttgc gctgcttttt cacgaatttg ctgcaattct tcagtagatt
     6541 tagctaatgc agcatccaaa cgagcttctt cagccgcagt atcttctact gttgccttac
     6601 tgaatgttaa atccggttga actagcaggt aagcttttgc aacggctaca ccgtcactag
     6661 cggcaatccc ttttagcatt tcaaccatta ttcagataag ccttctttct tcattgtttc
     6721 aacgattgct gccattgcat cagcttcgtc agcaccttct gcagtaatag taacatcaga
     6781 accttgacct acacctaaag acataacgcc catgattgat ttcaagttta ctgatttacc
     6841 tttatactct aagtttacat cagagttaaa tttgcttgct gtttgtacta atagtgtagc
     6901 tggacgagcg tggatcccag tttctgctac tacgtgaaat tctttctttt ccatattaat
     6961 cggtctcctt taagtgagtg ttgtttttat gtgagggttt tcttttttag taattcagag
     7021 aatcctaaca aatgccaccc tttcatctgt aaagattaac attttttttc gttagataca
     7081 acagttttca ttcaatttca gataaaagta ttcaaattat tccggattct ttttcatcct
     7141 tttcttcaca ttttataaca aacgtgttta tttccttttt cttgtcgctt tctcttctcc
     7201 tgacgtttat cattctttcc tttgaccctc tttgttttca tatgaaacga attacttgct
     7261 tcccttttta tttttagtat aataagagtc aaaggtcagg aatggtcaat agagatttca
     7321 ccttttctac ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc tcatgaaaaa
     7381 cgagatgtaa aatatggatg tacccatttt tgtgaaagga cgtgaatgat tcatgctttg
     7441 tcaaaactgc ggcaagaatg aggcaacgat tcacttgtat gccactgtca atggtcagcg
     7501 gacacaactc gattactgtc agagctgtta tcaaaaaatc aaaaatcaac aaaatggagg
     7561 tctgactaat atggcccaaa atgatccatt tggatttggc agtttggacg atcttttccg
     7621 ttccatgtct cgccaaatgc aacagcaagg aaattatgag caaacaccac caacacaatt
     7681 tggcggaaac aacaacttca atggtggtca gccacctcaa ggcaatacag aaggtttatt
     7741 gggagaatac ggaatcaata tcacagaaca agcacgacaa ggtgatatcg atcctgtgat
     7801 cggacgcgat gacgaaatca aacgagtcat tgaaatttta aatcgccgaa ccaagaacaa
     7861 tcctgtcttg attggagaac caggtgttgg taaaacagca gttgttgaag gtttagcaca
     7921 aaaaatcgta gatggtgatg taccgcaaaa attaatggac aaagaagtca ttcgtttaga
     7981 tgtcgtatct ttagtccaag gtactggtat acgaggacaa tttgaagaac gtatgcaaaa
     8041 attgattgaa gagatccgtc aagcagaaaa tgtgatccta ttcatagatg aagttcacga
     8101 aatcgtcggt gctggttctg ctggtgatgg aaatatggat gctggtaata ttttaaaacc
     8161 agctttagct cgaggagaat tgcaaatggt tggtgctact accttaaatg agtaccgcat
     8221 catcgaaaaa gacgctgctt tagaacgtcg tatgcaacct gtacgggtag atgaaccaac
     8281 tgtcgaagaa acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag actatcatca
     8341 tgtaaaatat actgatgagg ccatcgaagc agccgcaact ttatccaatc gttacgtcca
     8401 agatcgcttc ttgccggata aagccatcga tttgctagat gaaacaggtt ctaagaaaaa
     8461 cttgacgatc caaatcgttg atcctaaaac aatcgagaaa aaactgcaag aagcagaaga
     8521 acaaaaagtt ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt atcgcgatca
     8581 gatcaataaa cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa cacctgtgat
     8641 taccgaaaaa gatatggaaa aaatcgttga acaacggaca ggtattccag tgggtgaatt
     8701 aaaagaaaaa gaacagacac aactgaaaaa cttagcagat gacttgaaag ctcatgtcat
     8761 cggacaagat aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc gtgttggatt
     8821 aaacaaacag aatcgtccaa tcggctcctt cttatttgtt ggaccaactg gtgtaggtaa
     8881 aacagagctt gcgaaacaac ttgcctacga actattcggt tcacaagatt caatgattcg
     8941 tttcgatatg tctgaatata tggaaaaaca tagtgtctct aaattgattg gttctcctcc
     9001 aggttatgtc ggatacgaag aagctggtca attaacagaa aaagtacgga gaaatcctta
     9061 cagtcttgtc ttgttagatg aagtagaaaa agctcatcca gatgttttgc atatgttctt
     9121 gcaaatctta gatgatggtc gattgacaga tgcacaagga agaacggtca gctttaaaga
     9181 tacgatcatc attatgacaa gtaatgccgg aactggtaaa gtagaagcaa acgttggttt
     9241 tggtgcggca cgcgaaggtg ttactcgttc tgtattgaac caattaaaca actactttac
     9301 accagaattt ctgaaccgtt ttgacgggat catcgaattc agtgcattat caaaagaaaa
     9361 cttgatgaca atcgtcacac taatgctaga cgatgtcaac caaatgctgg cagcacagca
     9421 acttcacatc gaagttccta cgaatgtcaa agaaaaatta gttgacttag gctatgatcc
     9481 atctatgggt gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag atggtatcgc
     9541 tgaattttat cttgatcacc cttctatcca tgagttgaaa gcaaaactgg ataaagatgg
     9601 aaaaatcatc gttacttcta aaccagaacg tttggctaaa gaatcagccg aagaaacggc
     9661 tgaataaatc cagttttata gcgtgagacg gtatttcttt gagaaatgcc gtctcattct
     9721 tttttcagaa aagtcaaaat actgttcatt ttataaaaaa gaaattataa taagagaaac
     9781 aagcaattaa aaatacataa aagaggtgag cgccatgaaa ttggtaaatg taacaaacag
     9841 ccattcccgt cttgtattaa accagcttga aaacactgat gcccatctag tcaaggtcta
     9901 cacagccgga aataccacta ttgtctacac tgaagcccct gaacataatg aaattctgct
     9961 gatcaatgac aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa attatttctt
    10021 agataaaatc aaagacgccg tttaccacga ggatgaaatc aaagtaatcg aattggcagg
    10081 ccttgttgaa atctcgattc caaaacatga attaggcgct acattagccg tgtaaacaaa
    10141 ttttgaacat aataattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    10201 ctaaacacgt ataaacggcg aaagcagaag caactccttc ggaaataagc tgaaattcac
    10261 gaaaattgct tttcaaattt ttgtgaattc cagcttattt cttggagtta aacgcttctg
    10321 tctcagcctc tttttctttc tagctttttg atttttctct tttcttttgt acttttctct
    10381 tttcattttc agttctacgc gtgtctttat ttttaacttt ttcctcttta tggatgattg
    10441 atcgtacttt ttgtgcaatt tgctcgtttt tgtcccagtc ttttacatct aataatcgac
    10501 ataatcgtaa catctcttct tttttctgtt tttttctttc acgtctttgc cattctgttt
    10561 ccatgtgtca cactctcctt tctttaatca tatgtacgca aaataatttt tatatttttt
    10621 tgattagaca tttctatgat tttaatatag taaaaagagc cgtcacggaa aatccgtcac
    10681 ggctctaaac aaaatatgag aggctgtttt tgtttggcta ttcttattac ttgatgcagg
    10741 gcagcttgtg tcaagacttc tttacatcaa actttttaat tttacattgg gatatttatc
    10801 cgcaaaccag cgttctgcaa attggttctc gaataagaac aacggttggt cgaaacggtc
    10861 acgagccaag atattgcgac ttgaactcat cttctcatcc aaatcttctg gatcgatcca
    10921 gcgagcgatt ttgtgtccca ttggagacat gattacttcg gcgttatact catttaacat
    10981 acgatattgg aatacttcga attgtagctg accaacagct ccaatgatat attcttccgt
    11041 tagatatgtt ttataaagct gaatcgctcc ttcttgaact aattgataga ttcctttgtg
    11101 gaaggatttt tgtttcatga cattttttgc agtcactttc ataaacagct ctggagtaaa
    11161 tgaagggagt tcttcataag caacttttag ttttccttcg aacaatgtat ccccaatctg
    11221 ataattgcct gtatcatata caccgatgat atcgcctgct actgcttttt cgacgttttc
    11281 tctcgaatct gccataaatt gtgtgacatt gcttaatttc agttttttat ttgtacgttc
    11341 caaccagaca tccattcctc gttcgaatgt tccagaacag attcggacaa aagcgattcg
    11401 gtcacgatgg gctggattca tatttgcttg gatcttgaat acgaaacctg aaaattcttc
    11461 ctcataagga cttaccatct gatcatcttg tgttttgtgt ccataaggag ctggtgccaa
    11521 ttcaacgaaa gtctctaaaa aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa
    11581 tactggtgtc tgatcgccac gagcaatttt ttcttgatta aatgcatctc cagcctcttt
    11641 gattagttct acttcttcta agacttgttc atataaggaa ttttcagtta gaggatgatt
    11701 ttcgggaagc agtccatctt catccaattc tactaatcgt tcatcctgat aattttccgg
    11761 acgataaaac tctacacgat tatggtagat atcataaaga ccttctagcc ctttacccat
    11821 accgattggc caattcatcg gataagattc gatatctaat agctcttcta attcttctaa
    11881 caattccaat ggttcacgac catcacgatc tagtttgttg ataaaagtaa atatcgggat
    11941 gccacgtttt tttacaactt ggaataattt ctttgtctgt gcttcgatcc cttttgcact
    12001 atcgattacc atgaccgcac tatccacggc catcaaagta cgataagtat cttcagaaaa
    12061 atcttcatgt cctggtgtat ccaagatatt tactcgtttc ccttgataat caaactgcat
    12121 aacagaacta gtaacagaaa tcccacgttg tttctcgatt tccatccagt ctgatttggc
    12181 aaaattacct gtttttttgc cttttacggt cccggcttga cggatagccc caccaaacaa
    12241 tagtaattgt tctgtaatcg ttgttttccc agcatccgga tgggaaatga tcgcaaaagt
    12301 tctgcgattg tccacttgtt gctttaattt tggattattc ataacctaac tccatttctt
    12361 ccattcaatt atttatattt tctctaggca cagattgtcc gatctcttaa agaaagacaa
    12421 tttcatatag ggctgtgaca aaagccgtgt agcagtctct ttttccgagt aaacggcgtt
    12481 ctagagacag cctatcagca ataagcccac ccaagccaaa aatacgaaaa gctgtttttg
    12541 cttggctgct cttattactt aatgcaggat agctttttca caacctttgt caactactac
    12601 aactgaatca gtatagcatg gattcagcga atgaaaaagc caccaaacca taagagtcaa
    12661 atattttttt gtcagctgac taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg
    12721 tctttcataa ccaagtttcc aattatcttc ttggctcgtc atcttcgaat tcttttctga
    12781 agtatcttcg ttcttcttca ggttccgctt ctgtttcttc cggatcatga aaaatgacac
    12841 ggattttaag gattcgagaa ccttccattt cttcagaaac cagcgtaata ttatcgacat
    12901 caaacgataa cttttcgcct tcatccggaa tcatgcctaa tgctgtaatc aaataaccag
    12961 ccatcgtatc tacatcactc atatgcagat cactgtcaaa agcttcattg aactcatcaa
    13021 tcagcatacg tccttggatg atatattcgt gttcatcgat tttttcataa agattctcaa
    13081 cttcatctga ttcatcatcg atttctccga cgatttcttc taaaaggtct tctagtgttg
    13141 ctaacccaac gacgccgcca tattcatcta ataaaatagc catctgattc tgcgtctttt
    13201 tcatttcata taacagatca tcaataaaga ccgtttcagg aacgaataaa ggctcctgca
    13261 tgattttctt gatatctaaa ttatcaaagc cgaatttatg ggcagctttt agtaaattct
    13321 ttgtatgaag gattccgact actttatctt tgtcttcgtt ataaacaggg attcttgaat
    13381 agttttcgga taaaacttca ttcacatttt cttccacagt gtcgttgata tcaatcataa
    13441 atgcatccgt tcgtgggacc atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc
    13501 cttggagcat ttctaattct tcattattca atacgccttc gttttcaagc atataacgca
    13561 tttcatctct ggtcattttt gaatcttcat catcaaaagt catcggcgtg atacgcgcca
    13621 ataaacttgt ggaagcagaa agcagccaga cgaagggctt agctactaca ccaatcaagc
    13681 gtatcagccc tgaagtgaac tttgcgactt cttctgattt atttaaagca atacgtttag
    13741 ggtacaattc tccaaaaaca atcgatacat aggtcaaaat agccagcaca atgatatttg
    13801 caatattttt tgccgctgtg ccgccaccta ataccggagc taaccgttcc gataaagtat
    13861 tagctagtga agcaccagac aaaatgttga ccaaagtgat tcctacttgg attgtcgata
    13921 ggaaattatt tggattctgc aatattttta aaagcttttg agatttcaca tctccttcat
    13981 cagctttttg ctcgattcga ttcttattga tcgaaacaac agcaatttcg gaagctgcta
    14041 aaaaagcatt gatcaatgtc aaaacgatca ataataaaat ctgcgctaac agcgactgac
    14101 tctcagggtc agcattcata atcgcattct ctccttataa aattaatttt tagtttacga
    14161 tagaaaaaaa cgtggcacta tagccacgtt aaaatggtat cacaaagcaa ttttctttgc
    14221 aataaaaaca agtgtactaa aactacctac gaggtcgtag caattttctt cttgttttca
    14281 ttttgcttgt cttctttgac gattttgatt atataggaaa tgatcgtctt gcagattgcg
    14341 taaaaaggaa cacctaagaa gataccaagc aatccggcaa tatttccagc tactagaaga
    14401 acgataatga tcgttaatgg atggattttc agtgatttcc caattacatt aggataaatg
    14461 atattcccat ccaactgttg gacgattagc acaaccacac aacaaagtaa tgcttttacc
    14521 ggatcattga agactgttac tagaaatgca ggtgctagtc ctagataagg tcctaaatat
    14581 ggaattaggt ttgtcaatcc cgcaatgacc ccaaataaga aagcataatc cacaccaatt
    14641 accatatatc ctaaaaatgt gaaggtacca acgaataaac attcgattgc ttgtccgcta
    14701 atgtaattag ccaatgtttt attcagttgc cccaataact ccacaatttg gtttcttctt
    14761 ttttctggga aaaactgttg gatgtttggc actagttttt ctccgtcttt caacatatag
    14821 aacaagatga atggtgctgt aatgattact acggttgccg aagcgatcgt tccaacgatc
    14881 gaacctaaac tattggatac tccactaagg aattgctgaa tgattgtccc gtaagaaatg
    14941 tctaattgct caaaatattt attgatatct acttccttga acaaaggcaa ttcggctacc
    15001 tcttttaacc agctttctac ctgtttgatg aaatcaggca tattagatgc caatgaggaa
    15061 atttgctgta ctagacttgg gatgacacta agtaaaatcc aaataattgc tgcaactaaa
    15121 aggagcagca caattgcgac agcccatatt cttttgattt ttgctttcat caacagcttg
    15181 acaataggat tcaataaata gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg
    15241 gaaaaaaatg tcccgatcgg ttcgaaaata aagtttattt ctgatgaaat catgatcaat
    15301 gcagctatga tcaataattc aagcgaccaa aacattagtt tagactgtct aagtttttca
    15361 aacaatagat ttcctccttt ctttcttact agtatatatt atattcgtta aaaattcttt
    15421 tattcttttt ttatttttga aaacttgcca tactttttat ttgttttatt ttccagtata
    15481 ctatacgaaa ggaaggtggc tagaccatga aagttgtaca aacaaaagat acaatgagca
    15541 atatttactt agatgctgta cggatcaggc atcaagtttt tgtagtcgaa cagggtgtgc
    15601 ctttgtctcg agaaatagac aaagatgaag cccattgtat ccacttcgtc ctgtactcag
    15661 ataaaaaaga accgcaagga actgtacgtc ttttgccttt agaaaacgga aaaatgaaat
    15721 tacagcggat ggcgattctt tctgaataca gacaccaagg gttaggaaaa atattgattg
    15781 aagaagcaga aaactttgct aagaatcaag gctataatac gattttatta ggggcacagt
    15841 ccacagctga aactttttac gaaaaactag gatatacagc ttacggcgat ccttttgaag
    15901 atgctggcat gcctcatatt tatatgaaaa agactttata aaaatttatt tcgaaaaaag
    15961 caaaaaacag caagcttttg agaaaaatct ctcactggct tactgttctt ttttgtgttt
    16021 acttacgcga caattgcttg taacggttat accaaatatc aatatagcct tccgagaacg
    16081 ggccatctcc attattgatc cagtcaacca gaatcttcac attttctttt aggatgaagt
    16141 caatatcgtt cgggtaattc atcattttgc tgtgcatctc atattcatcg acatccaata
    16201 gtcgtttctc gccgtcagga aataccttga tatccaagtc atagtcgata tatttcaagg
    16261 cctcatcgtc cagcaaaaaa ggtgaagcta gattacaata ataggaaacc cccttttctc
    16321 tgatcattgc tattacattg aaccagtatt ttttatgaaa ataaacaatc gctggttcgc
    16381 gcgttaccca tcggcgtcca tcagattcag tgactaatgt gtgatcattc acgccgatca
    16441 tagagtgttc gcttgttttt aataccatgg tatctcgcca cgttcgatgc aatttgccgt
    16501 cgtgcttgta actttggatc gtcacaaact ctccttcttt tggaactccc atagcaaccc
    16561 ttccttaagc agcattttgt gctttgtgtt tgggtgttca ttacaccgac atcataatta
    16621 ttatatcaat aaaaatgagc tttgcctaga aaggattcct attgtttcga tttttctcta
    16681 actgatctac cagtttcatt tggaccttag gaaaaacgac cgaatcaaat gcatcaggct
    16741 tcaaccactt cgaagttttg ttctcggtaa attcttgttc tgctccctcg gttgcccgtc
    16801 cgtaaaaaag gagaacatgc catttcagat gactaaaaat atgagtaact tctcctaaat
    16861 gtcgtgtctg ccaaacaaag agttcctgct tttcaaatgg gagattttgc tcttcttctg
    16921 ctactaggtc atcaaacaga tcgatttctg gctttgtttc ccattctttt ttcaatcgct
    16981 catactcctc ttgtgtcact tccatcattg gaaatgtcca catattcgcc agcaattttt
    17041 ggctatctct ttcttcaaaa taatacgctc cagaatgatt ttgaagagct gcactgatat
    17101 aataaacgtc ttttggcttt gctttttttg tttttacagg aaaactcgtt tggattcctc
    17161 gtttgttagc taaacaaaag gcttggatcg gacaagtctc acactttgga gaagttggtg
    17221 tacagatagc tgaacctaga tccatcattg cttggttgaa ttcacctgga tgcttttcat
    17281 caatgatttt ccgcatcgct tcatcaaaga ttttcctgct agaagctttt gcgatgtctg
    17341 cttcaatgca aaacaatctg ctgactactc gcatcacgtt gccgtcaact gcaggttctg
    17401 gaagtccaaa tgcgatactt gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg
    17461 agctaatttc ttcaggcgtt tgcggcattt ccccatcaaa ttctgacatg atctgtttag
    17521 cagcagcttg gatgttccgg gctcttgagt aataaccaag gccctcccat gcttttaaaa
    17581 gcttttcttc tggagcattt gctagctctt caattgtagg aaaccattcc atgaaacgat
    17641 aaaaataatc gatcactgtg tctactctgg tttgctgaag catgatctca gagatccaga
    17701 tacgataagg atcacgatta tagcgccaag gcaggtttct tttttcttgt tcataccatt
    17761 gaatgaactg atcttgaaat tcttttgttt ctttatctgt ccatttttcc cattctttat
    17821 tcactttcct cttccttttc cgtaatgaat cgctggatca tatccaaatc aaatcctttt
    17881 tgatataagc tttgctttac ttttagattt ctttttttag gttcaaacct taaatttctt
    17941 cgccatagtt tatctccttg tttgactaaa taatcatatt ctatttcttg atctgcttct
    18001 tgcggaagat cagctatcgc ttgttggatc acttcttgag aaaagccttt cgtcatcaat
    18061 gtttgttgga tttttctcaa taattctttt tggctttttc cgtgattttt tccaaacgct
    18121 ttttcggctg tctgatgtgc attttgaact tggtcttcaa atgtatattg gctgagcgct
    18181 tgttcaatga tttcaggttt caaacctttt tgctgcatct gctgcgctaa tttctttggt
    18241 cctttgtcac ttaagcgcat attcgtgcgc acataacttt ccccatagac caaatcatcg
    18301 acaaggtcca attcttttaa gcgggctacg attttatttc gatcttcttg aaggatttcc
    18361 ttttctttta agtatgttcg gatttctttt tccgatcgca actgataact taagtaattc
    18421 attgctaggt gaaaaccgaa atcagctccg ctactcttct tgatctcttg gatagtttct
    18481 tcgtctaatt cccgtccttt taataatcga tagcgtacta atacatcttc agatacttgt
    18541 aaagcttctc cttcagataa atctacgcga tagaaagggc ctttgccttt actgattctc
    18601 gtaacggtta acataaacat cctcctcgaa catatattct ctatcatacc acagaaaatc
    18661 cccgtaatca ggtattatat gttagaataa accacagaaa aaagaaaaag ggagatactt
    18721 atggcagaaa caattgtcaa aactggacaa acggttcctt tgaaaatcaa gcgtttaggc
    18781 ataaatggtg aaggaatcgg ttatttcaaa cggttgatca tatttgttcc atatgcacta
    18841 ccaaaagaag aagtactagt gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa
    18901 ttaataaaag tcaaaaaaac aagcaaagat cgcgtagtgg caccatgtcc tgtttactat
    18961 gaatgcggtg gctgtcaatt acaacactta gcttatcaag ctcaattgga ttttaaaaag
    19021 gatctgcttt tgcaagcatt ggaaaaattc aaaccagcag gctttcgatc ttatcaactt
    19081 ttgccgacga tcgggatgga tgagccttgg cattatagaa acaaagcaca atttcagtta
    19141 cgaaaaaaca agcagactca aaaaattgaa gctggtttat acgaagcaaa ctctcatcag
    19201 ttagtcccta taacagactg cttggttcaa gaacctcgaa cacaaaaagt aatgaatact
    19261 gttgttcaac tattgaataa atttgatgca cccatctatg atgaacggac aaacagtgga
    19321 atcttccgca caatcatggt tagaatcggt gttaaaactg gggagttaca agttgttttt
    19381 attactcgca gtaaaaaatt cccgcaaaaa aacgcaatga tccgtgagat caatgaacga
    19441 ctgcccgaag ttgtttcaat catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg
    19501 ggggatgaaa cgatccactt atggggaaaa gaagccattg aagaacaaat caatgaagtg
    19561 actttcgatc tttctcctcg tgccttcttt caactcaatc ctcagcagac cgaattgctt
    19621 tatggcgaag gaatcaaagc attagatgtc cagcctaatg aaacgatcgt ggatgcttat
    19681 tgcggagttg ggacgatcgg cttaagtgta gcaaaacaag ccaaagaagt acggggaatg
    19741 gataccgttc ctcaagcaat cgctgatgca aaaaaaaatg cggaaagatt aggatatcac
    19801 aacactcgat atgaagtcgg gacagcagag caagttctgc ctaaatggtt gaatgaaggt
    19861 tttcagcctg atggtatcat cgtagatcca ccacgtacgg gtttggataa cgcattgatc
    19921 caatcattat tgaaataccc tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca
    19981 cttgccagag atctggttaa tttaagtaaa aagttcaaga ttgaatattt acagtccgtc
    20041 gacatgtttc ctcagactgc acgttgtgaa gttgtcgtga agatgacgag actcgctgac
    20101 taaatgaaag aggttgtgaa aaagtctgcc ctgtatcaag taataagaac agccaaacaa
    20161 aaatagctct tcatgtttta tgaatactgt acttgtgacc gaagagttaa caggaggaca
    20221 ctatttattt aaaaaaaggg ttgtgacata gtttttgtca cagccctttt tgattattat
    20281 tggatattca cgtctatcac ttgataaaat gcatttgtcg tatcgctgat attccatact
    20341 gctaaaatca cattatatcc ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt
    20401 tttggtggaa cagttgcttt gtcgtctatt tttgtgatca attcaaaatc actaaatttc
    20461 aaaggttgat taggattcca agttggtttg gtaatgtagt aatcccaagt acttgtccga
    20521 tgttgtgctg ttaaattcca agtaatattt aatgttcctg gattaaccat gtttttatgc
    20581 catctgttag atgtctgttc atctaaaggc tcaaagccgc tgactcctgc actagcaatt
    20641 tttccatcaa tgaatgtatt tttggtagct tcgatacttt gcggttccca ttgtgctctt
    20701 ccgacatttt gatttaagtt tcctgctgct gatgttccta ggtatgcacg gcttcctgga
    20761 gaagtaatat aaccatgcgc aaaacctgga atgctaaaac cgaacaaccc taaactaact
    20821 aacccaataa acaataacgt actaatcttt ttcatgtaga aacctccttc atttgataac
    20881 cacattgtaa acgctttatt gtattaacac aaacattatg cttttataat tttatgttga
    20941 agtaaaaaag gtgaaaaaag taagatgcag attataacag aagtagacga gaaaagtaca
    21001 aatagaaaac atgagaatca aatcatatga tctttttcaa aaacatcatt catataaaca
    21061 aatgttcaac tttttaataa caacaattat caattttttt cttacttcac aaactaaata
    21121 caggtcttgc tgtgagcctt catggtaaac taatattata gaaaggagtt agtaaaatga
    21181 agatgataaa agatattcgt ttttgggttt gtgttatagg aattgtcata ttaggctttt
    21241 tatcagggtt gcttagcgga aatcccggaa aatattacta ttctttgcaa cttccaccat
    21301 ttgcaccacc tagctggata tttgggccga tgtggacctt actgtatata ttgatgggta
    21361 tttctcttta tttgttactt aatcacaaca acaaaaaaca gcgaaacaat cttgtcggtc
    21421 tttttgtgat tcagttcatt tttaatttta tatggtctgc tctatttttt aatttaagaa
    21481 atattttcat tgcagcaatc gatattacac ttttagttat tttcttgtca gtgcttatgt
    21541 atcagttgtg gctgcatcat cgcctggcaa tgtggctcat gattccttat tatttatggg
    21601 tactattcgc aactctattg aattattcaa tttatttttt aaactaagcg ctaaaaagag
    21661 acgtcctgta atttaacaca tagaaaaagt gccacctaag ttagattttt tgtctaattt
    21721 aggtggcact ctttattaac caaactattt ttttctagta cccacttgtt gttccatact
    21781 cattattctg gtcataacca taaccatttt gttcctgtcc agcgtaagga tcataacttt
    21841 gagtatcttg acttggtgta tagtactgac ttgtatcttc tgtttgctga taataggtat
    21901 tcggatcttg gtactgatca tatgtgtttt ctgaatagga agaataatcg gtgtaccctt
    21961 cactattcga tccatcaccg tttgtcgaat cgccatcttc cgaatcatca aagaacatca
    22021 ttgaagcatt ctcatttttc aaattaggta atgtatcgct tgtttttata tttaattgtt
    22081 tcttcagttc attttggatc gaaagaagat cattttttcc taaaatttga tagtagatat
    22141 cattgatcat ttgtccttcg ccttgcaact ggtcttgttt gattgtttca aacgccgggg
    22201 tgtagttcgt cgctacatca agcatgtcat cccaatctaa gtccgtcgtc acattgttct
    22261 caactgcttt taaaattttt ctataattgc ttacgccatc taaacttaaa actttattga
    22321 cgattttcgt cactacttcc cgctgacgac gttgtcgacc gatatcgcct tctggatctt
    22381 cgtgtcgcat tcgagaatag gctaaacctt tttctccatt caatgtttgt tcacctactg
    22441 gtacatggac accatccaaa gtaaagtcaa ttttattatt cactttgatg ccaccgaccg
    22501 cattgatcaa atcttccatg ccatccaaat tgatcgttac ataatgatca ataggaatat
    22561 cgagcagttg ctcaattgaa tccatggcca tttcgacacc accaaatgcg taggcatgat
    22621 ttaatttatc taccgttcca taacctacga tattcgtata aatatctcgg tccaaactga
    22681 tgatcgttga ttttttttgt ttcggattaa ctgttactac catcatcgta tcagaacgtc
    22741 cttgttccgt tcttcctaat ccaccggtat ctaaacctaa tagcaaaacc gaaaaaggtt
    22801 ctttgtcatc aatgcttact ctctcagaac gtttggagga tattcgatta gagtttttag
    22861 aaattttgtt tactgtttgg ctcgcttctc ccataaatct gattccgtat gctgataatc
    22921 cggcaatcag caaaacgaat acccctaaca aggttaatag aactttttgc catcgcttca
    22981 taatacacct ctgtttcaca ttttctaaaa tattgtctca tataactgtt caatcagcaa
    23041 attcactttt taatcataag aaaactttaa aaaacatttt tgtgtaacga ttattatttt
    23101 ttaaaatttc tcaaaattct taactatcat accataccgt gaaaaagatc gaaagaccga
    23161 atacacgcag ttcgcaatcg ttttatcttt ttttatgtat tttttccaga aaaagcgaac
    23221 aacaaggtat tttccaaata atcgtgcagt aaaatgatcg cacatacctt tcccatcttt
    23281 ttaacagtct ttgcctaccg ttcagattat cattcgattt cacgacaatt tcttcctatg
    23341 actggtcctt ttgttaaacc catcttctat tcgttttatt tccagctcac tgcatggctc
    23401 ttaaaatgac tcgaactagt gattgcatta ccatcatctt ttaatggata gatatgctcc
    23461 tcttcaaagt aaaacgattt tcttttaaag taatagaatt gacatggtaa tgagtatgta
    23521 tactagtggt ttcacaaatg gtcttttatt caacatccaa aaactgaaat ttctttaaaa
    23581 tccattacta acgaagaaaa tgttccttgc ttgataatta gttaggacat gtcaagtgaa
    23641 caaatgcatt tacttagcca aaaaatctaa aaacttcata aatagacaaa aagacacgac
    23701 tctctaagaa gtcgtgtctt tattcaacaa attattataa tttagtcact tcagcagctt
    23761 gaggtccgcg tgcaccttca acaatcgtga attctacttc ttgaccttct tctaggctct
    23821 tgaagccgtc tccttgaatt gctgagaaat gaacaaatac gtcatctccg ccgtctactg
    23881 taataaaccc aaatcctttt tcattgttga accatttaac cgttccgtgt tccataaatg
    23941 tatagcctcc aaaaaagttc ttgcaagtaa ccttgcaaga acattatacc caatttagaa
    24001 aacgctagca agctttcgct cttttatttt ccaaattatt tttaagactg atatgacgga
    24061 ggaaatggtt attttgctgc ttcgaaacgt ttgtttactt catcccaatt aataacattc
    24121 cagaatgctg caatataatc tggtcggaca tttttatatt tcaaataata agcatgttcc
    24181 caaacgtcca aacctagaat tggtgtcttt ccttccatca atggagaatc ttgatttgca
    24241 gtagaggtaa tagctaattt tccattttcc attacaagcc aagcccatcc agaaccaaat
    24301 cgtccagcgg ctgctttttt gaattcttct ttaaaagaag aaaaatcacc aaaagcttca
    24361 ttaatcgctt cttttatttc tcctgtaggt tcgccaccag catttggtgc catgatttcc
    24421 cagaaaaatg aatggttagc atgtccgcca ccattgttac gtacagctgt cttgatatct
    24481 gttggaatag cgtccatatc agataataat tcttctattg ttttttcgcc taattctggg
    24541 tatttctcaa ttgctgcatt taaattcgtc acataagtat tgtgatgctt atcatgatgc
    24601 agatgcatcg tttcttcgtc aatataaggt tccaatgcgt catatgcata aggtagatct
    24661 ggtaatgtgt aagtcataaa aaaattcctc ctcaatgttt tcttaataat gtctacatta
    24721 caaagagtaa caaagagaag gaaattcttc aattgttatg cctagtcgtc tttttcactt
    24781 tgacctactt cttcttcaaa aacctcagtt tctttaaaaa taaacaactt gctgaaaata
    24841 tagttcgcaa ggataacaat aatttgagtg atgattttcg ccacatactc attgctgttc
    24901 ataacattga tcaacaatac cattgccccc atgtccaatc cgtaagaaag gatacggtag
    24961 aagaagaact taccgcattc aataagtgca tgtgtaaaat tcggtgattt tgaatggaat
    25021 acccaaagtt tgttcgttac aaaagcaaac aatacagaac aaaaccacga aatagaattg
    25081 gataaggcca tacttaaatc aaaatattgg cgtgcgagag caaaactaac aaaatttaca
    25141 atcgtagcca aaccaccaaa aaagagatag actagtatct cccaatatcc ttttgcttct
    25201 aaatatcctt ttaactgtcg atactgcttc atgagccaac ctaccttatt aaaaattctt
    25261 atttatcata ctctatattt caataacttt catctttatt taaaaaaatt aatttttttt
    25321 gatcctttgc ctttgtctct gcttggatcg tcacatgtgt aataccatat tttaatttta
    25381 ataaagaaat tatacgagga tatatctttt caatctcgct tagtagctga tcttcaaaat
    25441 caatatgtcc gtcgaaaata attatctttt cgctatactg ccaaatatgg acatgatgaa
    25501 cattctttac cccatcgatt gcttcaattt cttgttccat tttttcatag tcaatagcag
    25561 gtgcactttg tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata
    25621 tatatatcga aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga
    25681 tcaaaattgc tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat
    25741 aactagattt tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca
    25801 aattcgcgag caatccaata actgctactg ttagcatcaa atgactattt atcttttctg
    25861 gtgaagtgaa ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga
    25921 atgagttgat gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat
    25981 ttgcttcttt tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac
    26041 taaggttatg gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa
    26101 attctgataa agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc
    26161 tctgtggctg tgtaggcact attccttcct ccattttatt tatattggat agtctttaga
    26221 caagaataac atttttttga tatttcacca atcattcaga tgtttcccta aagcacttgt
    26281 atttcacttg atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta
    26341 tgacaacaaa acaattaatt atcagttcct ttatacgatt caaagaccta aaggagacga
    26401 gaaatgtccc attttggaaa gccattgtct atcttttagt tttgagtata attatggctt
    26461 tgccaatctc ttttcaagtt tttcaagtct taaacaacat tcaattggat gggcaaaaga
    26521 ttgctactcg cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag
    26581 aaggattcat ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa
    26641 cagaacagga catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa
    26701 acaaacttat catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc
    26761 aattagaact tccctacacc aatgaatctt taaaaaactt aacaggtaag gagctccgtt
    26821 cttttttaag taaagcatct ataccacttt ggataaaagt cctgacattt atattttcta
    26881 tttatcctag ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc
    26941 tctatgcacg ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct
    27001 attcaatttc attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg
    27061 acacaagcgc cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat
    27121 gggcaaaaat atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac
    27181 ccttttagag gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct
    27241 ttctcaaaaa acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa
    27301 agaaaggcgg gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg
    27361 atgaatttat cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac
    27421 aaccagagga tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa
    27481 aactattagc ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc
    27541 taccccttga gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca
    27601 gtgaatcgat tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc
    27661 aagcacaaaa agcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta
    27721 tccaaggaaa aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa
    27781 aatgcaaagc attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag
    27841 gaatggaaaa gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg
    27901 tagtcaatat cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg
    27961 aacactttga tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac
    28021 aagatctggt gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg
    28081 aggaagagcc tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt
    28141 cccacattgc aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca
    28201 atctgaaaag ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag
    28261 gatattaata gctgaaaaca atattttcaa aagaacaaag agaaggtgac aaaac
//
LOCUS       DAFCLG010000023        28277 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFCLG010000023 DAFCLG010000000
VERSION     DAFCLG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.023"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..643)
                     /locus_tag="LND56_000975"
     CDS             complement(<1..643)
                     /locus_tag="LND56_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6742916.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITS"
     gene            complement(1211..2278)
                     /locus_tag="LND56_000976"
     CDS             complement(1211..2278)
                     /locus_tag="LND56_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6742917.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2298..2777)
                     /locus_tag="LND56_000977"
     CDS             complement(2298..2777)
                     /locus_tag="LND56_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBK6742918.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2794..3255)
                     /locus_tag="LND56_000978"
     CDS             complement(2794..3255)
                     /locus_tag="LND56_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6742919.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3269..4519)
                     /locus_tag="LND56_000979"
     CDS             complement(3269..4519)
                     /locus_tag="LND56_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBK6742920.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4897..6348)
                     /locus_tag="LND56_000980"
     CDS             complement(4897..6348)
                     /locus_tag="LND56_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6742921.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6669..7433)
                     /locus_tag="LND56_000981"
     CDS             complement(6669..7433)
                     /locus_tag="LND56_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBK6742922.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7461..8159)
                     /locus_tag="LND56_000982"
     CDS             complement(7461..8159)
                     /locus_tag="LND56_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBK6742923.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8171..8950)
                     /locus_tag="LND56_000983"
     CDS             complement(8171..8950)
                     /locus_tag="LND56_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBK6742924.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8966..9910)
                     /locus_tag="LND56_000984"
     CDS             complement(8966..9910)
                     /locus_tag="LND56_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6742925.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9956..10735)
                     /locus_tag="LND56_000985"
     CDS             complement(9956..10735)
                     /locus_tag="LND56_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742926.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(11115..13190)
                     /locus_tag="LND56_000986"
     CDS             complement(11115..13190)
                     /locus_tag="LND56_000986"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK6742927.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(13192..14109)
                     /gene="glyQ"
                     /locus_tag="LND56_000987"
     CDS             complement(13192..14109)
                     /gene="glyQ"
                     /locus_tag="LND56_000987"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK6742928.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(14495..15313)
                     /gene="recO"
                     /locus_tag="LND56_000988"
     CDS             complement(14495..15313)
                     /gene="recO"
                     /locus_tag="LND56_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK6742929.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(15456..16355)
                     /gene="era"
                     /locus_tag="LND56_000989"
     CDS             complement(15456..16355)
                     /gene="era"
                     /locus_tag="LND56_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK6742930.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(16370..16765)
                     /locus_tag="LND56_000990"
     CDS             complement(16370..16765)
                     /locus_tag="LND56_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBK6742931.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(16749..17225)
                     /gene="ybeY"
                     /locus_tag="LND56_000991"
     CDS             complement(16749..17225)
                     /gene="ybeY"
                     /locus_tag="LND56_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK6742932.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(17242..19434)
                     /locus_tag="LND56_000992"
     CDS             complement(17242..19434)
                     /locus_tag="LND56_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBK6742933.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(19458..20429)
                     /locus_tag="LND56_000993"
     CDS             complement(19458..20429)
                     /locus_tag="LND56_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK6742934.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            21135..22514
                     /gene="brnQ"
                     /locus_tag="LND56_000994"
     CDS             21135..22514
                     /gene="brnQ"
                     /locus_tag="LND56_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6742935.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(22670..23116)
                     /locus_tag="LND56_000995"
     CDS             complement(22670..23116)
                     /locus_tag="LND56_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBK6742936.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(23143..23319)
                     /locus_tag="LND56_000996"
     CDS             complement(23143..23319)
                     /locus_tag="LND56_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK6742937.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(23481..23909)
                     /locus_tag="LND56_000997"
     CDS             complement(23481..23909)
                     /locus_tag="LND56_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6742938.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24001..24861
                     /locus_tag="LND56_000998"
     CDS             24001..24861
                     /locus_tag="LND56_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBK6742939.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(24955..25305)
                     /locus_tag="LND56_000999"
     CDS             complement(24955..25305)
                     /locus_tag="LND56_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6742940.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(25321..27048)
                     /locus_tag="LND56_001000"
     CDS             complement(25321..27048)
                     /locus_tag="LND56_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBK6742941.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(27262..28188)
                     /gene="trxB"
                     /locus_tag="LND56_001001"
     CDS             complement(27262..28188)
                     /gene="trxB"
                     /locus_tag="LND56_001001"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK6742942.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
BASE COUNT         7900 a         5982 c         4768 g         9627 t
ORIGIN      
        1 aacttgttat ttttgaatgt tgcaaatatg aaatatggaa tatgatatct tcagatatat
       61 aatccctttc tgacggaaac ttgatattgt gctgttgaat tatctcagat gaatacagcg
      121 catgccaaac tgacattggc aatataaaat cgactttata actcggctca gtgccaatca
      181 ttccaagcaa cactttttca attatagcgt tatttgtaaa tatcgtacta tcatatcttg
      241 ctggctttga aataatatta ttatcatcat aatattcata atatccagca taacatgtgt
      301 ctgcattact cctttttata ccatttaaca ttgcttcaat ataattagct gcaacaaaat
      361 catcagaatc aataaaagca acatattttc cttttacatg ttcaagtcct gtatttcttg
      421 ccaatcccaa gccagcatta tttttatgaa taatacgaat ccttgaatcc tttaatgcat
      481 agtcttcaca tattttatta gatgaatcct tagatccatc atccacaatt ataatatcta
      541 ggttcttata tgtttgatta cacactgatt ccaaacaact tcctacatac ttttcaacat
      601 tatatacagg tatgattaca ctaattagat cactcgaatt catccttcta tcctttcact
      661 cacgttttct caattagtca atgacggcat caacgatttt cttaaattct ctgaaaaaaa
      721 tagactctga tcttgatgat gtgaatgaca tactatcttc ttctagaaaa attatttcct
      781 ttaaattatt tattaaatca ataatatgat cctcgtaagt atgctttccc ttggaataat
      841 gaaaatatac ttttgtatta cacaatattg caattttatt tcttaatatg attatttcac
      901 tcattcatta ctgccacata tacacaactt ataaccaccc cctgcaaagt ttagggtaag
      961 ttatcagata atatcttcct cttcatcatc cagaatacaa aatctattta tttatagaaa
     1021 tatcattaat gtttttatat aatgtaccgt gcttgtactc ttgctctatt acaagcacta
     1081 aaacactact attcatttga attcttacac ggataaaaat ttacatcgtc tatttcaata
     1141 ttttttctct ctattttatt cacttaataa gttcattaca taatcgaaac attttttatt
     1201 ttccttgtca tcactccttg ctcccttttt gatacagatt taaaattcta tcaaagaaat
     1261 ttccatcgtt aaatttcgtt attgccaaat ccttcgaaca acgtgaaaat tttacgtatt
     1321 tatctttatc attgattttt agtattgcag atgtcaacgc tgatacgtca ccaggttgaa
     1381 acaaatatcc attttctcca tcacgtactg ctgaagaaat atctcctaca tctgtagcaa
     1441 ttacaggcat tccataagaa atcgcctcta gaatagatat aggcaatccc tcgttatagg
     1501 acggtaaaat catcacattt gatcttctat agcactcctc ctttttttct cccacaaccc
     1561 aacctgtaaa ttcaaaatac gattgcaaat tatattcttc tatcaattct ttgagcgata
     1621 tttcttcatc acctgctcca gcaatctcaa aaataaactt tttttctgga tattgttctg
     1681 caatgttttt aactgcttct accaaatcgt gtacaccttt tcgctttatc aaaactccca
     1741 taaacagaaa tcggcacatt tttgaattcc actttatggt atattctgga actacaatag
     1801 aattttctac aacaacagtt ttacaatttg gctcaatttc tttaattatc ttcttccatt
     1861 tttctccaag tacaatcgtg gaatcagact ctcttaacag caagcgaatc tgattttgtt
     1921 tttttacaga aacactatca taccattttt taaattccga tccatgaaga tggatgatta
     1981 caggaatatt taataatcta aaccacttat tcagcacata tttcctacta aaactccctc
     2041 tgtacgacat gtgcatatga ataatattag gtttactcaa taaagagata cctataattt
     2101 tagcaaatgc atacataaaa tataaagatt taaatattgc atttccaccc ttataactcg
     2161 gtataaaagt catctcgata ttcttttttt cccaattaaa attgagcatt tgtgtaatta
     2221 tacttgtaat tccacctttt accgagctgt cattaccaat cataagtact ctttgcataa
     2281 cgctttactc cttatattta tattttttct attaatacct ctatcgaatt aataatcgtt
     2341 tccgtgttag atacatactt gttaaacatc cgaactcttg tttctttaac ggcatcttct
     2401 aaattttcta attcataaca aggatagata atattcatct catcaaattg tctaattaac
     2461 tgcagttggt gatcatcgac atgctcacca tacttagcca atcgaggaac agcaatgacc
     2521 cttttacctt tttttacggc cccaattata gcacctgtac ctccatgagt aatcaccatt
     2581 tctgcttttt gcatagtttc agtaaactca tccctattta gaaatgcttt ataggaataa
     2641 tttagtggtt tataatcact ataaccactc tgtgcaaatg tactttcgat tattactcct
     2701 ttttcaatta aatcatcaat agcttttaac aatctatcaa attgaaattt ttgtgaacct
     2761 aatgttataa atatcatttt tttctcctat tatttaatat atcccaccca aataaatagc
     2821 attaggataa aattgtagca tagtttccca ctgaacataa aattgatctg caaaatgata
     2881 catcaatttt cctgtctgag tagggctggt tacttttgca aacgattcaa tataaatcag
     2941 tttttttccc atcatttttg ttattaagca gatagggatc atagctaaaa cacctgtaca
     3001 aatcacgaga tccggttttt cctttatata tataataatt gatttgaata aattaataag
     3061 tatagtacac aaaaaaaact tttcttttct atttacttgt ctcatgtagt acgttttttc
     3121 gccttttgac tcagctgaat attctgtctg ttctgttata acaaaactct catacttctc
     3181 catcagtggt tttaacatca ttaactgttc ataatgcccc ccagaggaag ccgcaaaaca
     3241 tatctttttg ttcatattaa cctctctatc agtcacgctg aaaaatatct ctagtgtaaa
     3301 ctttttcttt aatatcatcc aagcaggtat catatcgatt ggcaataata gaatcacaca
     3361 tcgccttaaa ctgctctaaa tcgttgataa ccttacttcc gaaaaaagtc tctccatctt
     3421 tcagtgcagg ctcataaatc accacagtcg cacctttagc ttttacgcgc ttcataactc
     3481 cctggattga actctgacga aaattatcac tgttgctctt catggtcagc cggtatatac
     3541 cgattaaaac tggattttcc ctgttgacat catactgatt atattcagcg tagttgtaat
     3601 agcccgccat ctgaagaaca cgatctgcga tgaaatcctt acgggtacga ttagattcaa
     3661 cgatagcctc aatcaaattt ccaggaacat ctgcatagtt tgccaacaac tgcttggtat
     3721 cttttggcaa gcaataacct ccatatccaa aggatggatt gttgtagtga gttccaatac
     3781 gaggatcgag acaaacacca ttgattatct gctgagtatt cagccccttc atttcagcat
     3841 aagtgtctaa ttcattgaaa tagctgactc gcagtgctaa ataggtattt gcaaacagct
     3901 ttactgcttc ggcttcagtg aatcccataa aaagagtatc cacgttctcc ttaattgcac
     3961 ttttctgcag aagtgttgca aaaacatgag cagcatttaa caagcgctca tcagtcatat
     4021 cagtaccaac aataatacga gatggataca gattatcata taaagccttg ctctcgcgca
     4081 gaaactctgg actaaaaata atgttcttac tccccgttgt ctcacggact gattcagtgt
     4141 agccaacagg aattgtagac ttgatgacca tgatggtatc aggattcacg tccatcacaa
     4201 cctcaataac tgactcaaca gcagaagtat cgaaatgctg agtacgactg tcatagttag
     4261 tcggtgtagc aataacaacg aaatctgcgg atttataagc gctccttgca tccaaagtag
     4321 cagttaagtc aagttctttt tctgtaagat acttttcaat atattcatcc tgaattggag
     4381 acttccgttt attaatcagt tcaatctttt cagggataat gtctaccgcg gttacctggt
     4441 gatgctgcgc caatagtgtg gcgatactca aaccaacata accggtgccg gctactgcga
     4501 tgttgtactt tttcttcaca atatctcctc tttcctctct taagtatgga gaatgttact
     4561 cctacggcta ttcatcatgt caaaataaca cagcttagaa tgttgataaa ataattttcc
     4621 agaatgtgat aaaatctctt tcatttataa accacacatc taattttatc tattctccac
     4681 aagacatgta gagtattctt taataacaag atacgacctt actaaatact tcaaaaaatt
     4741 atcatattta attcataaat ataaatcttc attttaaatg tatgcccaaa aatttatctg
     4801 taatgcgtca tcaaaatcta gattccaaat ctttatcaat ttttatccac tcattctacc
     4861 actttagtaa tattttctat ttataacact ttcgtcctac attgatcccc ccctctgaag
     4921 tacagttttt actgtcttaa ataaaatttt aaaatctaac cccatactcc aattttttat
     4981 atattccgtg tcaagttttg ctacttcttc aaaatcagta atatcacttc taccacttac
     5041 ctgccacatt ccagtaattc ctggcttgat agcaattctt gcacgatgat gataatcata
     5101 ttcttcatac tctgatttaa ggcatggtcg aaatcctata aggctcatct ttcctgttag
     5161 aacgttgaaa aattgaggga attcatctaa agacgtggat ctaataaatt gaccgattcc
     5221 tgtctttttt gttccatctg gaagtatttt atttccgata attcttggat caaactccat
     5281 cttaaacata agattattgc tcatcttatt ttctttcatt aattctactt ttctttcttc
     5341 tgcatccata tacatacttc taaacttata catcttgaac ggtttaccat tctgacctat
     5401 acgttcttgt gcaaagaaaa ttgggcctgg tgaattaata taaataagtg gagcaataaa
     5461 gataaaaata attcccgttg caatacatcc aacaatacct gcaagaatat caattgttcg
     5521 ttttataaat gcctgttgag ttgtcataac attgatactt gttgtaagta ctgtatattc
     5581 tcctatacga ccaatagatt gttttcccaa agcactatct gatacttttg caagattcaa
     5641 atgtacagta aggcccattt cagagcatcg ttcaataaat tctttcatac acgaagtact
     5701 tttattcaaa ataataaaga cttcatctac ccatccctga cataagtaat ccgctgcatt
     5761 tttggaatca gaaacaactg tcactccctc aacatcttca ccaatcatgc ttttatctat
     5821 gataatgaca ccatttattt tatacatttt atagttatgc tctttcacat tacgaataac
     5881 tacttcagca atactactag atgtaactat aataagtgat ttgttcccct catttttcat
     5941 tttatgtttc aagtaatgtt tccataagag gcgagttata tatgtaatta caacgtataa
     6001 acctcccatc aagaaaagaa cgactctaga ataatcattt ccaccctgaa cactaatgag
     6061 atacaaactt cctaacagca ccaacataat cgcttgcttt acagaagaag caaattctat
     6121 atagtaccct ctttttaata cacctctata tgcttcaaac atgaaaataa ctaaaagatc
     6181 tgccagttca atgaagactg ccatgttttg atacattgga atcacataag gattccaatc
     6241 tacctggcgt aagacatacg atagataaaa agcaagctgg atacacaata aatctaaaat
     6301 aataaaatca atatgtttaa cccatcccct tgaacttttc ttgtacattc ctttttactc
     6361 ccctcttttt aataaaataa attgtattac ttgttttctc tcctaaatat ctttctgaag
     6421 gattattgac tcgacaaatt ggcttgtgga tattcttata tatagaacat ccgtaagcca
     6481 acttaaaact ccttaacaaa atttttttac tcatattgta gatcaaaaca agtatccaag
     6541 aaatcatatt ttatttcttt tttctgctta ctcaacaaca gctaaaagaa aaatcatcat
     6601 aaacaaattc aactaagcca acgatcccct tgtgaaatca agttttttct tgatttacga
     6661 ataaagagct aaaataaacc aaacttcttc ttttttacct cttcatagac tggataactg
     6721 atttcatccc cgttgatcag atcctttgcg acttgtttca tctgtactac tttttcctta
     6781 ccaaagtcct ttgcaatctg ctcatagcac tcttttaagt aaaaggtgcg cttagagacc
     6841 ccatgggcat cagaagcagc catctgcacg agattatgtt tgaccatcgt tttggctgtt
     6901 ttctggatgt ctttgccaaa taccccaaca taacttggcg cagtgagttg ggccaaacat
     6961 cccatatcta ggaacggaag caaatgattt gggtcttcgc gaaaatgcac gttgcgttct
     7021 ggatggacga ttactggtgt ctttcctagt tctaacaact caaaaaaaag ccgttctgca
     7081 tataacggca cttccagtgt cggaaattcg atcagaagat atgtatcatc taaatctgta
     7141 aataatattt catctcggtg gatgtcttcg atgagcgttc cggtaatacg tacttcttgt
     7201 ccttccaata acgtcaaagg caactgtctt ttctctaatt cggcttgtaa cgaagcgact
     7261 agagaaatga cttgtgattt ttcattgctg tattttccat tattgtggtg tggtgtgcat
     7321 aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca ttgcaatact tgcttctaag
     7381 ttttctgccc cgtcatcgat tcctggtagg atatggcaat gtagatcgat catttctctt
     7441 gtctcctctc ttcttattcc ttaatttccg tagtaataac cagcatcttt gctatgttct
     7501 gctccgttgt acacaacacc taagactcgt gcttgtacca tctccagcag ttcttttgct
     7561 ttagtcaagg attctttacg tgacatattt tcacgtacga ccaataaagt gccatccgtt
     7621 tttaaagaca tgatctgtgc atctgttacg gctactactg gtggcatatc gaatatcacc
     7681 acgtcaaata actgtcttgc ttcggatagg atctgatcca ttcttggcga gcttaacaat
     7741 tctgatgggt tgggtggctt aggtccacta ggcaaaatcg acaagttctc gactggcgtt
     7801 cgttggatca catctgctac acttcctgat gaactaagtg ctgtacttaa tccactggca
     7861 ttgttcagct ggaatgtctt atagacgacg ggtttacgta aatctgcatc gactagtaga
     7921 actcgttgac ctgatttagc aaaaacgact gcaatgtttg ctgcagtggt ggatttccct
     7981 tctccaggac ctgatgaagt cacgacaatc gttttgattt gctgaccagc tgcggaggca
     8041 aactggatat tcgtccggat cgtacgatat tgttcggcga ttggtgaaga tgggtttgtt
     8101 aatgtgatca aactgactgc atgagtttgt gctgcttttt gtttctgtgt tcgtgccatt
     8161 tttcttcctc ctagactctt gagcggcttc tgcgtgatgg ggctgtttta tcgtttgtag
     8221 cttttggtcg tgcaggagct gttccttttt tacggatacc agcgttcaat tctttcgctg
     8281 acatttctgg aactgttcct aagattggga atcctaatgt atctgtcaca tatttttcat
     8341 ctttcactgt ccgatcgagt aattccaata agaatgccag acctacacct accatcattc
     8401 ctaacattaa gccagtcatt aaattcaatt tattatttgg cgaaacaggt gtcgtattgg
     8461 caactgcacc tgaaatgatc gagattttat caacattcat cacatctttt gcgttctctt
     8521 ggaaaacttg tgaagttgtg ttagcgatct gttgagcagc cactgcattc gtacttattg
     8581 cctgaatcga gaacatttgt gaattttctg cctgattaac tgagatggca tctttgattt
     8641 gttcagcagt cattttcaca ttgtcttctg tttctaaacg atctttgact tcattgagca
     8701 ctaaatcact gacgatcata tctttatacg tattgatcat ttgcaggttc gtattgacat
     8761 cattgacatt tgcagtgtct gattgtggca gtgtcacgat caattgtgct tgtgaactgt
     8821 attttggtgt caagatgaaa aatgttacga ttcctgagat ggccaagcca gcaaacattg
     8881 agatgatgat tattaccaag tgtttgcgta atgtttcaaa aatttctttc aagctgattg
     8941 tctcttccat tttttctcca ctttcctatt tattctctag attcatttga tcttttaatt
     9001 cttttttcac acgatcaagt tcttgatcac tgactttttg ataggagata ttatcttgca
     9061 tgaagccttc tccttgcagc tgatcttgct taatcgtacc aaatgcctcc cgataatcta
     9121 aagcaatttt tttcatttca tcaaagttca tgtctgtttt catattcgac tctagagcat
     9181 tcaaaacgga ttgatagttg ctgacaccgt ttagactcaa tacttttttc gctacgcctt
     9241 ctacgatctt tctttggcgc ccttgtctac cgtaatcacc atttgggtct tcataacgca
     9301 tacgagaata agataatgcc tgatccccgt tcaatgtaat tttaccgatt ggaaagtcat
     9361 agccatcttg tgagaaagtg aggtcattat tgacttcgat accaccaact gcatcaacga
     9421 gttcctttag tcctttcata ttgatagaga catagtgatc gattgggatg tctacatatt
     9481 tttgtacggt atccatcgcc atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt
     9541 cttttgtgtc acgacctacg atgtcgacat acgtatctcg aggaatgctg acgatcgttg
     9601 tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt atctgatctt ccttgatcga
     9661 cccgacctaa atctccagta tcaatcccca tcaatagaac agagaaagat tcttgtttgt
     9721 ccagatcaac tggtttcgtt cgttctcctt gggttcgttc cactgattcg taagttttct
     9781 caatagaatg tgagagatcc agatataatt ttgctcctac tcctacaatt gctaatacta
     9841 atacgataag aactgctaaa ataatcaaaa atattttttt ccctcttgat tttcttcttc
     9901 gtcttgccat agtttcctcc gtttgctaac cttgcttgaa tcaacgtttt aactgtcaat
     9961 agcttatatt gctatagccc atttgctgag caacagtttg tgcttttgtt gcgtatgcgg
    10021 ctcttatttt ttcattccgg atctgctcta tttgactgag cgccgtcaaa tattgatcgt
    10081 aagtagcggt agctgttact tgaccgtctt tcagcatcgt atcgaatagc tgatctagtt
    10141 gcgtgacttg tgcaatttgg gtatcagcga atcctagata ttgcataacg actgttttcc
    10201 aagcactatc cttaaaaaag ctcatatttt cacgtacatg ggcgacatct gcacttgcca
    10261 acttttcttt gatgatgaca tcatccacag cttgctgcca gtttgatacg tttttagtaa
    10321 ataatttatt gacagccgtc tggatcttca gttttgattc tgcatcctct aactgcgtta
    10381 agactgcttt cttttcttct tggatcgcag cagcttcttt aggtaattct gattccttga
    10441 tgtcataatc atctgccgaa agtttgatag aagatagttt atgtgacaaa tttgaaactg
    10501 tttcttctgt tatttcagga tttaggaaaa ttttttcttt tgtactgtac agcttatcta
    10561 cttcttttga taaagaggtc acggtgtctt tttgattggt gatggcagat tctgcgtaat
    10621 tgatccgttg atgatattgc cgctgattat tgagatggta tccaaacaag caaatcacaa
    10681 tagcgcctgt gcctaccgcc acagtagcca ataacttgac gaacggcttt tccatagatt
    10741 cactcctaaa tattgatgat agataagtaa agatgtgatt cattcacatc agaatgagcg
    10801 ctttttattt taaaagcata attaaactaa tatattcata gaaatattta taaacaggtt
    10861 ttccctatat aatcatacca catacagttt ttttgactag ggaaattcct aatttaaaag
    10921 taaataaaac agtaacaaga ttataattaa tcgttttcat ttttatttcc tcacttttca
    10981 tgagcgtttt gaaaattcga acaaagaata aaataacaaa aattattaaa atagaattaa
    11041 taacagctat ttataacaaa aaaaacagag acacagcacg tttcgtgcat gctctctgtt
    11101 tttcaaacaa aagactattt tgtgatgatt tgatccaagc ttgctaatga caaagccatg
    11161 gcagctattt gacttagttg tgttagtcgg ttcttacgta ctgctggttc atcagccatg
    11221 accatcgttt gatcaaaata attttcgatc agtggacgaa gagcgacaag ctggtcgtag
    11281 ttttcgtgga tcgtttggtc agcaaaactt gctttcattg cttctactgc tttgtgtaat
    11341 tctttctctg cgtcgttttc gaacagctca gggtctactt ctgattgttt ttcaagctgc
    11401 gcttttttcg ctaagttcac gacacgtgtc aaagcttcca ttgaaggttt gaagtcttca
    11461 tctgcaagat gttgtttgaa caattgtgct gctgcaaata cttttgtcag atctttttgt
    11521 tccgcattca aaacagcatc gatgacatca tgacgaatat tcttcgttgt caataattgg
    11581 cgcagacggc ctttgataaa gtcgatcact tgttgttgcc cttctgataa accaatgccg
    11641 aaacgatccg tatctttact gataacatcg ctaatcgttt cttgcagaac agacaaagga
    11701 aaggcccatt ttttcgattc gatgatccga acgacaccgt atgcttggcg acgtaaggca
    11761 taaggatcgt ttgacccgct tggaatcatc ccaactgcaa agaaagaaaa gatgctatct
    11821 aatttatctg caatcgctaa aacagaacct actgtagact gaggtagctc tccttcactg
    11881 gatataggca tgtaatgctc acgaatcgct gtcgctacag caggtttctc gcctttcaga
    11941 agagcatatt tttcgcccat gatcccttgt agttcaggaa attcccctac catgttggtt
    12001 actagatcaa atttatagat ttcagcagct cgttgcaaat ccatcaattc ttcctcagat
    12061 agtcctactt gttcgccaat gatacgggca atgacttgta cccgttgcat tttctcatag
    12121 atactaccga ttttttcatg gaaagtaaca gtttttagtt tttcaacgta ttggtcgatt
    12181 gaagctttct tgtcttcatt ataaaagaat tccgcatctt ctaaacgagc cgtcaatact
    12241 ttttgattgc ctcgaaccac atcttccaat tttacccgat caccattacg agcagaaata
    12301 aaatgaggaa gcagcatacc ttgatcattc cgtacctcga aataacgttg atgttctttc
    12361 atagaagtga ctagtacttc ttcaggaaca gaaagatatt tttcttcgaa gtcgccagaa
    12421 aagacagttg gatattcgac taaattgttg acttcttcta gtaaatcttc gtctagatca
    12481 ataacccagt gatttttttc tgctaattcg tttgcttgtg taacgatcat ttcttgtctt
    12541 ttttgcggag acgcaatgac gaattgttca ttcaattttt cttcatattc ttttgcatgc
    12601 gcaaatgtca catcttcgcc taagaaacga tgacctcgag aggctctgcc tgttttgaca
    12661 tccaagattt cgaatgggat tacttcgtca tctaacaatg ccacgatcca gtgaataggg
    12721 cggatatatt caaaatcgta ttctgcccaa tgcatcgtta cagggaaagt caaacttttg
    12781 atcacccctg ctaagcctgt caatacttct tttgcttcct taccatgagt gtatttcgtt
    12841 acatagacat attccacacc ttttaattct ctaaaagtga tagcgtctgt cgtcagacct
    12901 tgtccgcgga caaatccttc cgctgctttc gtccagtttc catctgcgtc caaagcaatt
    12961 tttttggctg gtcctttgac ttcttcttca ctgtccgctt gtcgttctgg aatatttgtt
    13021 actttgattg ctaaacggcg cggtgtggaa aatgtttcga tcgtatcata agtcaattgg
    13081 ttttcatcta aaaattttgc tgttttttct tctaattgtt tcatgcttgg tgtgacaaca
    13141 tgggcaggca tttcttccag cccgatttca agtaataagt cttttgccat cttattcagc
    13201 ctccttcacg gtttctggat ttgctgtgtt tttattcaac aaagggaatc ctaatttttc
    13261 gcgttctgcc acaaagattt tggctacagc acgagccatg ttgcggatgc gagaaagata
    13321 accggcacgc tctgttacgg atactgcacc gcgggcgtct aataagttga atgtatggct
    13381 acatttcaag atataatcat acgccggatg aacaagattt tcatcgatac agcgtttagc
    13441 ttctttttca aaacgatcaa agttgcttag tagcaagtct tggtcgctta cttcaaaaga
    13501 atatttggaa tgttcatatt ctggttgttt gaagatctcg ccatatttca cgccatcagc
    13561 ccattctaaa tcatagacac tttcaacttc ttggatataa gaagcaagac gttctaatcc
    13621 ataagtgatc tcagaagtta ctggtttaca aggcagtcca ccaacttgct ggaagtacgt
    13681 gaattgtgtg atttccattc cgtcaagcca tacttcccaa cccacaccgg cacagcccat
    13741 ggatgggttc tcccagttgt cttcaacaaa acggatgtcg tgttctaatg gatcgattcc
    13801 taaaaggcgc aagctttcta aataaagctc ttgaatattc tcaggtgaag gtttcatcac
    13861 cacttggaat tgatgatgct gatacaaacg gtttgggttt tcaccataac gtccatcagc
    13921 tggtcggcga gatggctcta cgtaagcagc attccatggt tctggtccga ttgcccgtaa
    13981 aaaggtatat gggctcatcg tacctgctcc tttttctgtg tcataagctt gcatcagcat
    14041 gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc atatcttgga cggtcaattt
    14101 tttactcatc tgttttgccc ctttcaattt taaagcgttg ctgtcaaata agaatacagg
    14161 aaagaaaacg acaaaaaatc cctatgtctg attacagaca tagggacgaa taattcgcgg
    14221 ttccacccta cttccgaata aagattcggc agcttcattt atcgtactcg caagtgcctc
    14281 ttcaaaaatc gtttgggtcc ggctcccact ctcccgaact cgctaaacca cgtgtttttt
    14341 actgatcttg gtcaatgtac gtattcattt tgcagttttc ttgtttctat aatagatcct
    14401 atcactgaac gatctattta atctatggtt aataatagac ggaactccct gtgtttgtca
    14461 aatggttttg attttttgtt gtatgtttta tttactattc ttctttttca ggtgtctctt
    14521 tttctttttc agacatgtct ttctctgacg tctttaatac gtgtgcccaa gacttcattt
    14581 gattgatgaa ctttttgctt ttcaaatgga taccgacata ttcatcatac agctggtcga
    14641 tgagttcacg gattgcttgt ttggtttctt ctttcagatg gattccacta attttatcat
    14701 aagaaatagc tgaaaacatc cgaacgaaat aaacagctcg aggatctgca tgataacggt
    14761 gatcgtccat cgaccaatgt ctttcgcata tcacaccacc atatttggaa gaatagtcaa
    14821 attttccttt tgtttccccg catacgacgc agtgtgtcca gattggtgca attccgaagc
    14881 gttgcagtat ttggacttca aaaatattcg tgatgatttc tgcatcgtct ccttggtcca
    14941 tccgttgcag tgcttgttgc gtaaactgga acaaatgcgg gtcgtactga tgatcttcga
    15001 ttgccgcatc tactagattc aataaatatg tgccatagcc gctcaaaaag atgtcttctt
    15061 ggatgtgtcg aaacgcatgg acatctttga cactgcttaa aaatgacaaa ccttcacttc
    15121 gaaaattccc gatataaact gcttcagtaa aagggagcaa agccggagct aagggctgat
    15181 tttgccgatg gatccctttg atataaaaca tcattttgcc agaagattcc gtaaagattt
    15241 tgactaattt atctttttct ttgtaattac ggctagataa aatcaagcct tttgattctg
    15301 caagctggct catattctcc ctcctaaaaa ataaatctgg aaaagcagat tctgccgatc
    15361 cagatcattt atgttgcgat cttccgcagc ttttctcaac acgcggattt ttatccagtt
    15421 tatttctata cctaacttac aagaagctca gaatatcaat attcgtcttt tcggtagccg
    15481 aagtcttgca gatagacttt tttgtctcgc cagtcttttt gaactttgac ccaaagttct
    15541 agaaagactt tgtctcctaa aagattttcg atatcttttc tcgctttcgt tcctacttgc
    15601 ttcagcattt ttccgccttt gccgataatg atcccttttt ggctatctct ttcgacaata
    15661 atcgtagcct ggatatggat tttatcattc tcgtttcgct tcatcgaatc aacaacgact
    15721 gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga ctttctcacg gatcaattca
    15781 gaaacgatga agtattcagg atgatcagtc acttgatcat ctggaaaata ttgtgggccc
    15841 tctggcattt cactgactaa tacatccatc aaacgttcga cgttgtttcc ttctgtagca
    15901 gaaattggaa tcacttgtgc aaacggcatc tgcgttgaat aatcttcgat gattcccaac
    15961 aactcgtctg gatggatttt atcgatttta ttgataatca gataaacagg agaatgcacg
    16021 ttttttagac gttcaataat aaaatcgtct ccccgaccgc gtttttgatc agcgctgatc
    16081 ataaataaga ccgcgtccac ttctttcaac gcactgtaag ctgcttctac cataaaatct
    16141 cctaaacgat gctttggttt atggattcct ggtgtgtcaa taaaaatcaa ctgagcttct
    16201 ggtgtagtat atactccttg gattttattt ctagttgtct gcgccttatc actcatgatg
    16261 gcaatttttt gacccacgat tcgattcaat aatgtggatt ttcctacatt cgggcggccg
    16321 acgattgcga caaaccctga tttatgttct ggcatatacg ttctcctttt taattaaaca
    16381 acaaatgata tattttaggg acaaataaaa tcaacccaac gatgacggaa aaacaagcgg
    16441 tcactaaaac tgctccagct gccatatctt tgattttttt cccaatcgga tgaaaatgaa
    16501 aatccgtaaa catgtccacg acattttcaa aaacagtatt gatgatctct actatccata
    16561 cgagaaaaac agctaaaagc agccataacc attctatccg atcaagctgg aaaatcacgc
    16621 ccataaacaa agcgaggatt ccgaacgcaa catgtttcct catgttgcgt tcttcctcaa
    16681 aaacggtttt gactccttgg acggcaaatt ccacggacat caggaaatgt ttgtttttct
    16741 ctacgttttt atcttttaag tccataggca tctaagatct ctttctgtaa accaaacatt
    16801 tctttctcat cctctggtgt catatggtca tacccgttga tatgcaagaa tccatgaacc
    16861 gctaaaaagc caagttcccg atcatacgaa tggccgtaag cttctgcttg ctctgcagca
    16921 cgttcagtag agatcatgat gtctcccagt acacgaggca atggattctc tccatcttca
    16981 aaaatcactg ggatttcgtc ttcgccctct tcttccaagg caaaactgat gacgtctgtc
    17041 ggtgcatctt ttccacggta atcacgattg attacttgga tcgctgcatt gtccatgaaa
    17101 gtaacagaca tttctgtttc ttctggcaac tctaaatatg atgctgcaaa ttgcagcaag
    17161 tcgtcgattt ctttcatttt ctcttctgag acttggtttg tttcatccat gaaagtaata
    17221 tccattatac atcgcttctt tctattcttc ttgttcttct tttatcttct caacttccga
    17281 tttcatttta ggatatttga tccgagaatg gaaggtactg cttaaaccgc tgatcaatga
    17341 tttttctact ttacggattt cttttaacgt caatccactg tcgtccaatt gtccatcgga
    17401 aatacgctct tcaataagat tatggacaaa actttcgatt ttttcgctcg ttggatggtc
    17461 cattgctcgt actgccgctt cacaactgtc agctatattg acgattcctg cttcacggct
    17521 ttgaggtttt ggtccaggat aacggaattg ctcttccgtg acttccgggt tgcgttcctt
    17581 tgccttaacg tagaaataac gcatcagcgt ggttccatga tgctgtctgc aaatatcgat
    17641 caccatttgc ggcatattat aatcttccaa tattttggca ccatctgtca catgtccaaa
    17701 gataatatgt ttactgtctt ctggcaataa gaaattgtgc ggattttccg ctcctgaagg
    17761 caagttctca acgaaaaagc tggcgtgttt gatcttgccg atatcatgat aatagcaagc
    17821 tactcgagtt agtaaagaac gtccaccgat ttctgctaca gcattagcac ttagattggc
    17881 taccatcatg ctatggtgat acgtgcctgg tgcttcttcc agcaactgtt tcaataaagg
    17941 atgattagga ttgctgagtt catttaatac gatcacacta tcatcattga ccatcaattc
    18001 aatatagggg tgcagaccta tcgtcagcaa gaatgagaac agtgcacctg tgaatccgca
    18061 tactagcgtt aaccaagtcg ttgcatcccc aaagctcatt ccttggtaga taatcaaaat
    18121 gacatccatc atcacaggaa aaataatgac ccacatagca gcggataacc cttgttctga
    18181 gatccgtttg cgtctgacga tcgttgccat gagaccagaa aatacatagg acatcaaaat
    18241 aactgtcaga aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg
    18301 aaaaatacca gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa caagtggagc
    18361 aaaagccgcg gggtaaaaca gcggaatata agcagcttgt tctgtttgga agagttggaa
    18421 aaatttcatt agtacgacgc ttactgacat tgccgtcacg tagaaaagta cgtattttgt
    18481 tctttctcct gcattttgga attgaagtga attataaatc agtacaccga tttgaagcaa
    18541 aacagctaaa accatggcaa tgagtgggaa aatagaagtc gtttgattcg tcaatcctag
    18601 taattccaat tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa caataatttc
    18661 tccttggaag atcatcactg gttgtacagc ttctcgtgca gattgtttta attcttctgt
    18721 ctttttctca ttcaatacat cattaactac aatgccttga tccactaagt agcgaatcat
    18781 ttgttgctgc tctgttgtga cattcaaata ttgaatctga tcttctgctt cttgtttaaa
    18841 agcttccaat tcgctttcac gaacttgttt actcatctgt tcgtcaatga gcttcaaact
    18901 ttcatcccta accgtgtgaa tttccgtttc agtcatttca aatatcgtcg aataaaaatt
    18961 gttcggcagt ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa aatttttctt
    19021 cagtgtagca atccgctctt ctgtagtagg tttcgggata ttttcatctt ccttcgcctt
    19081 atctaccttg tcattataag atttgttgat ggtatcattc gtcttatcga tcaattcgaa
    19141 taattgcttg atccgattgt tctggtcatc tgccagatct ttttgataag tatattctgg
    19201 agtcactgct tctgctgcca gttttcttct ttgttcggtt tcttccgtgt tctcaatcgt
    19261 tttattcgca cggatactct cttctgctac ttggccttct ctgaaatcat tggccttttg
    19321 tttgacacta ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca gaaggaaagg
    19381 caatgctgcc tttccgagtt ttttttgtag tttctgcagt gttttattca acatgtgctt
    19441 atcctcctaa gcaaagctta ttcttgatga agatccgcct tttcataagc tttgatgatt
    19501 tcggcaacta cagggtgccg aacaacgtca cttgcttcaa aattaacaaa atcgattttt
    19561 ttgatttgct ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc acgaggcaag
    19621 tctatctgac ttgtatcccc attgacaatc atttttgatt gataaccgag acgggtcaag
    19681 aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac gaatgcatca
    19741 tccaatgtcc gcccccgcat gtaagctaaa ggtgcgattt cgatcacgcc tcgttccatc
    19801 aatcggttcg tatgatccaa tccaaaaatt tggtataaag catcataaac aggccgcaga
    19861 taaggatcca ctttttcttt cagatccccg ggtagaaagc ctaggttttc ccctgcctcc
    19921 acagcgggcc gcgtcaaaat gattttttgc acttcgcctt tttttagggc agcaattgct
    19981 aatactacgg caagaaacgt tttccctgtt ccggctggcc ctacaccaaa tacgatgtca
    20041 tgtttggcta ccgattcaac gtatttcttt tgaccgctgt tttttacccg tataggtttt
    20101 ccattacggt ctttgacgat ctcttcctca tacatctcaa caaaataatc taatgtacct
    20161 ttttgcccca tcttcaatgc cgtcacaaca tctggcgtac ttacgtggat tcctcgtttg
    20221 atcaattcct gtaatgtccg aatgactgag gcagccaagg aaatctggga ttgctcacca
    20281 atgatctgaa tgatttctcc acgtgtatgg atggttgttt ccgttgtttc ctcgatcagt
    20341 tttatatgct tgtcgtgtgc acctaaaagc atgctgacat catcagcagc tgtcaatcta
    20401 atctctaaag aactagactc ttccgtcaaa aatagacatc cccttttttt cgattcattc
    20461 tttcctattt taccatactc agaagaaact atatagcaaa taagaaaagg ttgtaaaaga
    20521 gtcgtttaac catacgtacc aagaaacgtt aaaaaatagc cgtttccatt cgctaaaggt
    20581 ctcgttttga taaaaagaag ctgagaatgt aagttatatg aaagtggaga acaaaatatt
    20641 ccgtttcatt cagcaggtat tctctaggta tttttcttgt ttcaaaaaaa gaaaacaaaa
    20701 tagttaaaat attcttgcat ttcaaaaaaa acaggtgtat tattacgtca acaaataaac
    20761 atacagcaaa gcgtcgagga aaacagtagg ctattcaacg atttattccc agtgagtcat
    20821 gtaagtgaga aatgacaata aatcgaatag acgaacatca tttccgagtc aatgtcgaaa
    20881 acgtagatca ttgtggctgc ctccattatc aggctagcgg ataactttat caaaaagcgt
    20941 ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta ccgttctaga atctagagcc
    21001 cttttcccaa gtggaaaagg gcttttttta tattttaagg tggtggtttt catggaatct
    21061 gatattagtg gcattactaa ttgctgcaac gtttatatcg tcaatcaaaa tgataattat
    21121 tggaggaaaa atttatggaa gaaacaaaaa cttattcatg gaaacaacta ttgatggtca
    21181 gttcacttat cttcgggatg ttcttcgggt caggtaatct aatctttcct gtccacttag
    21241 gtcaaatggc tggcgcaaac tgggtcagtg ctggcgtagg atttgccatc tcgggcgctc
    21301 ttttcccatt actagctatc ttagctgtag tagtgacaaa aagtgatggt ctgtatgact
    21361 tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt aatccttgtt catttgacga
    21421 tcggaccatt tttcggtaca ccacggacag ctgctactgc ttttgaaatg gctgctaaac
    21481 catttttgcc tgaaaaatat aattcattag gtatgttttt attctctgct cttttctttt
    21541 tacttgctta tttccttact gtccatccca cacggttggt caaatacgtc ggaaagtatc
    21601 taaactcctt gtttcttgca ttgttagcaa tcatttttgt cgtttcttta ttccacccaa
    21661 tgggaaatct aaatcaatct gctgcagata cttaccaaac aaacgcttta acaaatggtg
    21721 tcctagaagg ttataacacg gttgatgcag tagccttgct tgccttaagt gtcacttttg
    21781 tccatgcagt ccgtgacctt ggtttcaaag ataaaaaagt cacagaatta acagcaaaag
    21841 ctggtagcct gagcgttgcg ctagaagtca tgatctattt tggtcttgtt cttctcggtg
    21901 cgatgagttt gaataagatg tctttatcag aaaatggcgg aactgcactt tctcagatca
    21961 ctaattttta tttaggtaac ttcggcgcta gcttcttagg agtgatggtc acattaggtg
    22021 tcttcactac cgctatggga ctcgttgttt cttttgcgca agatttccat aaactgttcc
    22081 ctaaagtcag ttatatgaca tggctgcggt taacgacttt cgtctctttt gtcgttgcaa
    22141 atgctggttt agacaatatc atccaatggt ctttaccagt attgatgctt ttgtatccat
    22201 tgtcgcttgc gttgatcttg ctttccttaa cagcaaaatt tttccaaaag acaccatttg
    22261 tctatcaagt aacgatgttg tttgctgctg ttcctgccgt attggatatg ctggcaaatt
    22321 cgcctgcttt agtgagtcaa caaagagttg tagcaagcat gctcgaattt tatcatcatc
    22381 acgttccttt tgctgcatta ggacttggct ggatggtccc aacgttactt ggttacgctg
    22441 gcagcctact gttttattac gcctatcgcc tatcagggta taaacaagaa gcaaatgaac
    22501 taccagaaga ataaccaaat caaacacaat ttaacataag aaatattaaa caggaaaaaa
    22561 ccagctagat gaaagaatcc tattctttcg tctagctatt tttttgttct cttttttacg
    22621 cgcacaaaaa aactgcggac aatgattgtc cgcagtttaa atgataaaat taagattgta
    22681 atagttcttt cacaatggca tttacttggt ttccatctgc ttttcctttg actttaggca
    22741 tgatcgcacc catgactttg ccaaattctt tcattgatga agcaccagtt tgtgtgattg
    22801 cttcttgaac aagttgtcga atttcttcgt cggaaagttg ttccggcatg tatttttcga
    22861 ctatcacaat ttcacttttt actttttcag ctaaatcgtc acgtcctgct tcttcaaact
    22921 catgcaaaga atcacgtcgc tgtttcattt cgcgagacaa caccgttaat tcttcttctt
    22981 ctgtcaagtc gtgaccttct ttaatctgct cgttttgtat agatgacttg atcattcgga
    23041 tgacttgtaa agattcctta tctttcgctt tcatcgctgt tttcatgtca tcgtttaatc
    23101 tacttagtag tgacacacta ccaactccaa ttcatcacag aactagaatt ttttgcgttt
    23161 tctagctgct tcagattttt tcttgcgtct tacacttggt ttttcgtaga attcacgttt
    23221 acgagattct tgtaaagtac ccgcttttga cacggaacgt ttgaagcgac gaagagcatc
    23281 gtcaagtgat tcgtttttgc gaacgactgt ttttgacatg ttaattccct ccctccgagc
    23341 ttaaaaagta accgtaattg ttattgaaat atatctcaaa aaacaagact gtcctgtaaa
    23401 tattatatct aatgctgttt tgaccgtcaa ggaaatcttg ctttttttta aaattatcaa
    23461 atgttatttt atttaaatcc ttattgacaa ttttcacatc ttccgaaaat ctcgaatcta
    23521 tgcccttcga tcgtacatcc accgagctgt tcttcgaaat aattcattgg acacatatgg
    23581 atttctttcg ttgttccgca caccgtacag ataaagtgat ggtgatggcc gatttcccct
    23641 tgacagcaat gaaaacgaaa cttcatctct ccatttaaat cggtttcttc cagtaaatcg
    23701 atttctgcaa aatcatggag atttcgataa atcgtatcgt aacttactcc tttgaattcc
    23761 tgattcatat agtcaaaaac ttctttagcg gatacgtagc gattgcgctt cactaaataa
    23821 gttaacaatg cttctctttt tttagtatat ttaaaaccgt tatttttcaa tgtttctagt
    23881 gattgttcaa tcaatgtctg ttttttcatg acttattcct ccaaatcaaa atgattacga
    23941 agtacacatt ctagagtatg gtataataaa cataaaaaaa atgcaagtga ggttttatcc
    24001 atgtcttccg attctacaga aatcgttact ttttttgtca tctctgactc tgctggagaa
    24061 actgcgacaa aattagcgca agccactatg gcccaatatc ctaccgttga gttcaattta
    24121 ttccgcagaa catttgtgac agatgagaaa acattgatgc aggcactgca agatgcacta
    24181 aaagaaaaag cccttgtcct gcacacattg atcaatcaag aattgatcga gatgacgcgt
    24241 tctttttgtg aagaacatca attatttagt tttgatgtga tgactccgcc gatatcagaa
    24301 atcgagcgct taactggtat caagcccatt cggcaacctg gcgcccttca tttattgaat
    24361 gagaactatt tcaaacggat caaagcaatg gaatttgctg taaaatacga tgacgggaaa
    24421 gatcctcgtg ggtttctaga agcagatgtc gttttgctag gcgtatccag aacttccaaa
    24481 acgcctttga gcttgttttt agctaacaaa aatttaaagg ttgccaatct acctttgatc
    24541 ccacaagcac atattcccaa acagctttgg gagatcgatc ctaaaaaaat cgtgggactg
    24601 acgaataacc cagatatttt aaacaatatc cgtaaagaac ggatgcgctc ttacggatta
    24661 aatcctgata ctgcttattc cgatatcgaa aaaatacgag ctgaactcga ttttgcgaat
    24721 gatctttatg aaaaattggg ctgcgtagta atcaatgtcg cttctttatc tatcgaagaa
    24781 acagcttcac ttattttgaa tgctttagac ctagaagatc atagttatta tggaactgaa
    24841 accaccgaag aaacagacta acagtttttc ttgtcttttt cttaaaaaaa ctggactgtg
    24901 tcagcgaaga agattcgttg atacagtcca gtttgtctct atttgcacct atcgttattg
    24961 atgttccaca tgtttgagcg tctggttcaa aatatccagt acgtgagaat catcaagact
    25021 atacaaaatc gcttttccct cacgatggga cttgacgaca tggttttctc ttaataaacg
    25081 cagttgatgc gatacagcag attgttccat ttctactgct tgggtgattg ctgtcacatt
    25141 ttgttccccg tctttcagat acagcagaat tttgagacga gtggggtcac tcaacatttt
    25201 gaatagttgg ctgactttct ctacttttgt tttttgcgtg tctggcaatc cattcacctc
    25261 caacttgaaa aaagactgac ttctaaagtt taccactttt tccatcagtc tcaagttttt
    25321 ttattcttct gtcatttttt tgataatcgc ttcataagcg gctacttttt cagctgcgtc
    25381 ttctgaagag tttcctttta caccgatata aaatttgatt ttcggctcag ttcctgaagg
    25441 acggatagcg atccagccat tttcttccag cgtatacttc aatacgtcag atggcggtgt
    25501 cgttagtttt tctacgtttc cttcacggtc tgttctcgtc aactctttga agtcttctgt
    25561 ttggatgact tttgctccgg caaattctgt cggtgcttct tcacggaatt tttccatcaa
    25621 agcagagatt tttgcagaac cttcttgtcc gctcattgtc acagaaatcg ttttttctgc
    25681 gtagtagcca tatgtttcaa aaatcgtttg caagccgtca tacaacgttt tgccttgttt
    25741 tttatagtaa gctgctactt ctgctaataa gagcaatgct tgaatcgcgt ctttgtcgcg
    25801 aacgaaaggt ttaactaaat aaccgtaact ttcttcgaat ccgaacataa aagtatggga
    25861 atgatcttct tcaaattgtt gaattttttc agcgatgaat ttgaatcctg tcaatacatt
    25921 gaacatggta gccccatagt gttccgcaat tgctgtaggc aattcgctag aaacgattga
    25981 tttcaatacc gctgcatttt caggtaaagt gcctcgcgat tggtgtgctt ccaaaatata
    26041 ttgagcaaac agggctccga tttggttccc tgtcaatact tcataggagc catttggcaa
    26101 acgaacggct gcgcctaaac ggtctgcatc aggatctgtt gcaatcagta agtcagcgcc
    26161 ttctttctcg ccaagtcgca tcgcatactc gaaagcagag tgttcttctg gattcggtga
    26221 tttgactgta gtgaaatccg ggtctgcgac agcttgttct ggaactagta cgaattgttc
    26281 gaatccagct tgtttcaacg cacgttcgcc caacattttt cctgttccgt gaagtggtgt
    26341 gtacacaagt ttcaaatctt tacccattgt ttccaccaat tcatgatcaa tcgttactgc
    26401 tttgatttct tctagataag cggcatccac ttcttctccg atgatcgtga tcaatccgct
    26461 atgttcagct tcgtcgtcag ataatacttc aacttttaac ggattgctga cttcacggac
    26521 atatttcgtc aatgcatctg cgtctgcagg aggcatttgt cccccatctt ctccgtagac
    26581 tttatatccg ttgtaagctg ctggattgtg ggatgcagtt accatgattc ccgcaaacgt
    26641 cttcaaatga cggactgcaa aagaaagctc tggtgttgga cgtaaacttt cgaatacata
    26701 agaagggata ttgtgatgtg ctaatgtttt ggctgcttcc atggcaaatt ctggtgattg
    26761 atggcgtgag tcataagcga tagcgacacc tcggcgtttt gtttcttcac cttgtgtatc
    26821 catgaaacgt gcaagtcctt ctgttgcttg tcttaccgta tagatgttca ttcgattgat
    26881 cccaggtcct aagattcccc gcatgcctgc agtaccgaat tccaacggtg cataaaaagc
    26941 atcttcaagt ttttcaggat cttcacccag atcaattaat tgttttttta agttttcttc
    27001 taaattttct tcatgtaccc attgttcata cacttgttcc caagacatga aaataacacc
    27061 tctttctgaa atatggttct taataagtat agcattcact tattagatat gaaagtacga
    27121 ggtctaaaaa acgcttaaat caagaaagga ttcttttatc aagcacggtt gcaagtattc
    27181 gcataaatcg gattcagtag atttcacgcg aaaaaaaaga gtaaaagaac aattgaaacc
    27241 gttcttccac tctttttaaa cttatgcagt tgtttcttct aaagattcca agtatttata
    27301 cacttgttgt ccagcgatcc caccatcacc aacagcagta gtgatttgtc gcaaatcttt
    27361 ttcacgaaca tctccgatgg caaaaacgcc tggtatattc gtcttcattt cgttgtctgt
    27421 tggaatccag ccagcttcgt tcgtcaaccc tgcttctcta aatggtgcag ttaatggatc
    27481 cagtccaaca tagatgaata ctccgtctgc tgccaaatca aactcattgc cggttttgac
    27541 attacgtgct ttcacacctg tcactaccat ttcattccca acgatttcgt caacgactgt
    27601 atcccacata aaggaaattt tttcattcgc aaatgcacgg tcttggatga tcttttgtgc
    27661 acgtaattca tctctacggt ggacgatgac tacttcagaa gcaaattgtg tcaaatagat
    27721 ggcttcttct actgctgagt ctcctcctcc gatcactact agacgtttgt tacggaaaaa
    27781 cgcaccatca caaactgcac agtaagaaac gccacgtcca gcaaattctt cctcgccagg
    27841 tacgcctaat ttacggtgga tacagcctgt tgcgataatg actgtttttc cttcgtaagt
    27901 tttatcttca caaataactt ctttgtggct gccgtgatcc ttgatatctt gcacgattcc
    27961 ataagcattt tctgtcccaa atcttgaaac accttcatac atcttataag ctagatcagg
    28021 acccatgatt gaatcgaaac caggatagtt ttcgatttct gccgtattgt tcatttggcc
    28081 gccaggggcg ccttgttcga tcatcaatac ggataagttt gaacgtgaag catataaagc
    28141 agcagtcatc ccagcaggac ctgcgccaat aacgattaca tcatacatat gtagattctc
    28201 ctttacttaa tcaagtaagc atactttaca gccaatgcga aaaaaagtct attgttctgc
    28261 ttgcgtttct cgaatag
//
LOCUS       DAFCLG010000024        27655 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFCLG010000024 DAFCLG010000000
VERSION     DAFCLG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.024"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..1342
                     /gene="lysA"
                     /locus_tag="LND56_001002"
     CDS             83..1342
                     /gene="lysA"
                     /locus_tag="LND56_001002"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK6742943.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1329..2129
                     /gene="dapF"
                     /locus_tag="LND56_001003"
     CDS             1329..2129
                     /gene="dapF"
                     /locus_tag="LND56_001003"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK6742944.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2132..3013
                     /locus_tag="LND56_001004"
     CDS             2132..3013
                     /locus_tag="LND56_001004"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK6742945.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3010..3726
                     /locus_tag="LND56_001005"
     CDS             3010..3726
                     /locus_tag="LND56_001005"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK6742946.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3732..4427
                     /gene="dapD"
                     /locus_tag="LND56_001006"
     CDS             3732..4427
                     /gene="dapD"
                     /locus_tag="LND56_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBK6742947.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4443..5444
                     /locus_tag="LND56_001007"
     CDS             4443..5444
                     /locus_tag="LND56_001007"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK6742948.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5441..6802
                     /locus_tag="LND56_001008"
     CDS             5441..6802
                     /locus_tag="LND56_001008"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBK6742949.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6762..7913
                     /locus_tag="LND56_001009"
     CDS             6762..7913
                     /locus_tag="LND56_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK6742950.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7888..9057
                     /locus_tag="LND56_001010"
     CDS             7888..9057
                     /locus_tag="LND56_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6742951.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9232..10611
                     /locus_tag="LND56_001011"
     CDS             9232..10611
                     /locus_tag="LND56_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6742952.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10892..11689
                     /locus_tag="LND56_001012"
                     /pseudo
     CDS             10892..11689
                     /locus_tag="LND56_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11878..12546
                     /locus_tag="LND56_001013"
     CDS             11878..12546
                     /locus_tag="LND56_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK6742953.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12746..13132)
                     /locus_tag="LND56_001014"
     CDS             complement(12746..13132)
                     /locus_tag="LND56_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742954.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13104..14486)
                     /locus_tag="LND56_001015"
     CDS             complement(13104..14486)
                     /locus_tag="LND56_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK6742955.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14642..15829)
                     /locus_tag="LND56_001016"
     CDS             complement(14642..15829)
                     /locus_tag="LND56_001016"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK6742956.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15836..16867)
                     /locus_tag="LND56_001017"
     CDS             complement(15836..16867)
                     /locus_tag="LND56_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6742957.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16883..18337)
                     /locus_tag="LND56_001018"
     CDS             complement(16883..18337)
                     /locus_tag="LND56_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6742958.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            18512..19417
                     /locus_tag="LND56_001019"
     CDS             18512..19417
                     /locus_tag="LND56_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6742959.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19508..20185
                     /locus_tag="LND56_001020"
     CDS             19508..20185
                     /locus_tag="LND56_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6742960.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20182..20352
                     /locus_tag="LND56_001021"
     CDS             20182..20352
                     /locus_tag="LND56_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742961.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20466..21362
                     /locus_tag="LND56_001022"
     CDS             20466..21362
                     /locus_tag="LND56_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6742962.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21417..22787)
                     /locus_tag="LND56_001023"
     CDS             complement(21417..22787)
                     /locus_tag="LND56_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6742963.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23025..23771
                     /locus_tag="LND56_001024"
     CDS             23025..23771
                     /locus_tag="LND56_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6742964.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23768..24607
                     /locus_tag="LND56_001025"
     CDS             23768..24607
                     /locus_tag="LND56_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6742965.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24608..25558
                     /locus_tag="LND56_001026"
     CDS             24608..25558
                     /locus_tag="LND56_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK6742966.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25699..26451
                     /locus_tag="LND56_001027"
     CDS             25699..26451
                     /locus_tag="LND56_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742967.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26470..27621
                     /gene="queG"
                     /locus_tag="LND56_001028"
     CDS             26470..27621
                     /gene="queG"
                     /locus_tag="LND56_001028"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK6742968.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9081 a         5036 c         5817 g         7721 t
ORIGIN      
        1 aatattattc caataaaatt caaatcttta tcaaatgaaa ttccaataaa aacggaaaaa
       61 aagtgaaaag aggaatgaaa aaatgacaga attgataatt ggcggagtca cagcaagcac
      121 attggcagaa aagtataaga cacctctgta tgtgtatgat gaagaaaaga tgagacaaac
      181 gatgctgtca ttcaagcgag gctttgtatc gaaacgtttt gatacaaaag tgctttacgc
      241 ctctaaagca ttccaaacga ttgagatgct gcatttagtt cgtgaatatg gattcgggct
      301 agatgttgtc agtggtgggg aaatctatac cgctttgcaa gctgattttc ctagagatac
      361 tatctacttc catggtaaca acaagacgcc agaggaactg atctatgcac ttgaaaatga
      421 tttgttgcat attgttgccg ataatttgat ggaagttgaa ttattagcag aattaagcca
      481 acgctattta aaaaaaattt gtatcatgct tcggttgaat gtcggagtag aagcacatac
      541 tcatgaatat atcgtcactg cgcatattga ttcaaaattc ggcatgagct atgaaagtaa
      601 agagtgccaa caagcccttc agttgataaa agaaagcagc tatctagagt tagaaggatt
      661 tcatgctcat atcgggtcac aaatttttga tgttactgct tggttagctg aaatcgacaa
      721 attgacgaat tacttaaccg attttgacca acctctttcc ttgaatcttg gcggagggtt
      781 tgggattcgt tatacacaaa atgatcagcc gatcccagtc gaagaggcac taaaaaaact
      841 gattgaatat acggaagaag ctttggaaaa acgaaaactg accattcgtc aattactgat
      901 cgaaccgggt agaagtattg ttggagaagc tggtacaaca ctatacacga ttggtttcat
      961 caaaaagaca ccacataaaa aatattactt tgtagatggc gggatgacag ataatatccg
     1021 tccagctctg tatcaggcag aatacgattg tgatttagca gcaaaactgt cggaagaaaa
     1081 aacagagaaa gtgacgattg caggaaaaat gtgtgaatca ggcgatgtgg taattaaaga
     1141 agcttatttg ccgaaagcag aatcgggtga tcttttagct gtttattcca cgggagctta
     1201 tggatactcg atgagcagca actacaatcg agcgacacga ccagcagttg tttttgtcaa
     1261 taatggccaa tcaagactga ttgttcgcag acaagcattt tctgatctac taagggggga
     1321 agtttcctat gatccttcat aaatataatg gctgcggaaa tgattttatc ttattagatt
     1381 acgcagaaga cacagattat agtcaattag ccgcttcttt atgcacaaaa gaaaaatatg
     1441 atatagacgg attgatcgcc gtaaaaatcg atccgttgga aatgatctat tacaacaaag
     1501 atggcagccg cgcgccgatg tgcggaaatg gcattcgctg ttttgctcga tacgtaaatg
     1561 atcagaacat gatgaatgaa caggaattca acgtagagac attagcaggc acaagaaaaa
     1621 ttacggtgtt ggatccctca cctttttatt gcagcgtaga tatgggagtg ccagatgatt
     1681 ctccggaaaa aatcaaagct aatcaaactt atccaataaa aaaccaggaa atagagatca
     1741 atggtgaaaa agtaacgatc actagtttat ttttaggaac gattcatacc gttgtttttg
     1801 ttgaaaatgc gcgtgaggaa gtctggagaa aaagaggtga actgatctgt catcatcctt
     1861 tgttcaaaga aaaaaccaat gtcaactttg tagaagtagt caatgaacaa gaattgatcg
     1921 tccgtaccta tgaaagagga gtaggttgga cacttgcttg cggtacaggt tgctgtgcct
     1981 cttatgtggt tgctaaggaa actgggaaaa tttcagcaga tcaggtagtt gttcacttgg
     2041 aacaaggaga cttgatgatc tctggtgagg atgcgataca aatggctggc ccagcagttc
     2101 atgaatggac aagaatattg gaggaatgaa gatgataaaa ggttcgatcg tggcgctgat
     2161 cacgccgatg aatgaagagg gatcagtaga ttatgctggt ttggaaaaat tgatccaatt
     2221 ccatttagat gaacaaacag atggattatt agttttaggt acaacaggag aatcttctac
     2281 gctcacacaa tctgaggagg agcagatcct tcaactgaca gtaaagaaag tggctggccg
     2341 tgtgccggtg attgcaggag ctggaacgaa taatacgaaa gaaacgattg aaaaagcgaa
     2401 gcattttgct tctttggggg cagacgcact tttggtcatc accccttact acaacaaaac
     2461 aagtgatgca ggactagctg ctcattttac agcaattgca gaagcctcac cgattccgct
     2521 tattttgtat aatgttcctt ctagaacagg catgtcgatt ccaatccatg tattagtaaa
     2581 tttagcagaa catcctaata tcattggttt gaaagaagcc tcaggagata tggcttatgt
     2641 gatggatgcc gctcggttga ttggagaaga attcttcctt tacagtggaa atgatgattt
     2701 gatcctacca gtaatgagtg taggaggaag cggtgtaata agtgtatggg cgaatatcca
     2761 acctaaaata gtccatgaat tagtaaaaga tacgcaggac ggaagatggc agcaagcaaa
     2821 agaaaagcaa ttaaatgcac ttgaactgat ccatgccttg ttcagtgaga ccaatcctat
     2881 tcctgtcaaa gcagccatgt cattattaga cttaccttcc ggacctctac gcctgccttt
     2941 agtttctctt tcaaaagaga aaaagaaaca attagctcag cttttgttaa aagaaaggac
     3001 ggttgaaaaa tgaaaatcgg actgattggc agtggacaga tgggaaatag agtcgaagaa
     3061 gttgcacaag aaagaggaga tacggtcctt cttttacgga cggcacctaa agaagcaact
     3121 acgcttgctt ttacggaact gccagaactg cttattgatt tttctcatcc agataattta
     3181 gagaaaatcc tttcgtatag cttatcatat caacttccgt tagtcattgg cacgacaggt
     3241 tatacacaat cacagttcca agaaataaaa gaacatgcca aacatgtacc tgtgatgtat
     3301 agtgctaatt tttcgttcgg catcatggtg atgaatcaac taatcaagca agcagctgca
     3361 atgttgcaag gctggcagat cgaactgatt gaaaaacatc atagtcgtaa aaaagatgca
     3421 ccatctggta ctgcaaatat gctgcttcaa acaatccaag aagtgcaaaa gctgcagccc
     3481 gtatacaact ggcaagggaa aaaaagagaa gaaaaccaaa ttggtgtaca cagtattcgt
     3541 gcaggttctt tacctggtga acatgatgta ttgtttgctg ctactgatga aatatttacg
     3601 atcaaacatg aatctttttc taatcgaatc tttgctgcag gcgcagtaga agcagcggac
     3661 tggttgaaag atcagtcacc aggttattac aaattagaag atttattaat ggacaaaggg
     3721 gtgtaaaaaa cgtggaagct caagaaatca tccgctacat tcaaacggca tctaaaaaga
     3781 cgcctgtgaa agtctatctg aatgtaaccg agccaatcga atttaaagaa agtaaagttt
     3841 ttggtgaagg aagcagcttc gttgtttttg gtgactatga gacgattgct ccggttttag
     3901 aagctgaatc agaaaaaatc attgaaatcg aaatcgaaac tgctgcaaga tacagcgcag
     3961 ttcctttgct agatattaaa aaaattaatg ctcgtatcga accaggtgct attatcagag
     4021 atcaagtgtc aatcggcaac aatgccgtga tcatgatggg agcgattatc aatattggtg
     4081 cagtaatcgg tgaaaacacg atgattgata tgggtgctgt cttaggtgga cgagcgacag
     4141 tgggcaaaaa ctgccatatt ggtgcaggag ctgtcttagc aggtgtgatc gaaccagcat
     4201 cggccaaacc ggttatcgta gaagacggcg tactggtagg tgcgaatgca gtgattgttg
     4261 aaggtgtcca tatcggaaaa gatgcagtcg tggcagcagg agcagtggtt ttagaagatg
     4321 tggcagcaga aactgttgtc ggtggaatac ctgcacgtgt attaaaaata agtgatgatc
     4381 agacaaaaga aaatacagca ttgattgctg cgttacgcga attatagagg agatggagaa
     4441 aaatgcgcga aatagatacc tgtattgtgg gggcaaccgg attagtcggc caaacgatgc
     4501 tcaaagtgtt agaagaatat gattttccag taggacgatt gaaattatta gcttcaaaac
     4561 gttcagccgg taaagtttgt gtattcaaga aaaagaaaca cactgtacag gaattgacag
     4621 aaacttcatt tgaaggatat gatttagcgc tattttctgc aggtgcttct gtatccaaag
     4681 agtttgcgcc aatcgcacga agtcagggac tatttgtcat agataattcc tcagcatggc
     4741 gggaagattc ggatattcca ttaatcgttc cagaggtcaa tctggaagac tatacattga
     4801 atcggctgat tgcaaatcca aattgttcaa ccattcaagc ggtacttccg cttaaagctt
     4861 tacaagaagc ctttggtatc cagcgagtca attattcgac ctatcaagca gtttctggtt
     4921 ccgggcaaaa aggcattgaa gatcttcatc gtacgcaaaa tgacgaggaa ccagttttgt
     4981 atccttataa tattagccaa acggtgattc cggaaatcga tcagcctttg gaaaatgggt
     5041 atacaaaaga agaacagaaa atgataaatg agacccgtaa gattctgcat cagcctgctt
     5101 tgccagtatc agcaacctgc gtacgtgtgc caatcgaaaa tggacatgga gtctctgtgg
     5161 cagttcaact gcaaaaagag tttacgacgg aagaaattag agagtgtctc agccagtttc
     5221 cagggattca attagaagat gatctagcag cacataaata tccgacgagc attcttgcta
     5281 gaggaacaga tatggtttat gttgggagga tcagaaaaga cacttcttta gagaacggat
     5341 tgttgttcta tacgactgcc gataacatac gcaaaggagc cgctgcgaat gccgtacaga
     5401 ttgctgcagc tttaatgcag aaaatagcgg aggtggcaag atgagtaata cgctgaaagt
     5461 agcgaaattc ggcggaacat cgatgtctca tgaagagcaa ttcaaaaaag tccgtacaat
     5521 tgttctttca gatcccaatc gccaagtgat cgttgtttct gctttaggca aacgtacgtc
     5581 agatgatgac aaagtaacag acttacttta tttgatttat gcttaccttc agcatcatgt
     5641 tgattggaca cccctttgga aaaggatttg tgatcgtttc attcaagtaa gagatgttct
     5701 tgccctgcag ttacctattg aagatgaact agcacgcata cagaaacaac tggaagacga
     5761 aaagataact gaagattatt tagtaagtag aggtgaatat ctgactgctc aactgatggc
     5821 tgagtatctt ggttaccagt ttattgacgc aaaagacctg attttctttg actatgatgg
     5881 gaaaatcgat caggaaaaaa cagcacagga aatcacaaaa gcatttaaaa gatatccgaa
     5941 aatggttgtt ccagggtttt acggggcgaa ccctgcagga aaagaaaaat tattgggccg
     6001 tgggggaagc gacattaccg gcgcgatact ggcgaagatg ttgcaagcgg aaagttatga
     6061 gaattggacg gatgtttcag gaatcatgat ggctgatccg cgaatcatcg atcatcctaa
     6121 aatcatcgag gaaatctctt ttagagaact gagagagatg gcttatatgg gggctaacgt
     6181 tcttcatgaa gcagctgttt tcccagttca agaggctgat atccccatcc aaataaaaaa
     6241 taccaatgat cctttcgctc aagggacaaa aatatcgaat cacgaaatag aaaaggaaaa
     6301 tggactgaca ggaatagctg gacgcaagga ttttctttcc atcactttat tcaaacgtca
     6361 tatgtccgat gaaatcgggt ttatctggaa agcaatgagc atttttgcta aacatgagat
     6421 atcaatcgaa catattcctt cagggatcga caatatcgga gtgatcgtct cagcagaagc
     6481 gattgccgat aagttatttc tcatcacgaa agaattaaaa gaaacacttg gcgtggaaga
     6541 aatcgtcgtg atcgaagact tagctttgat ctctgtcgtt gggggtccgc ataaagaatt
     6601 gattggatta tctgggaaag tgttatcgat attgaacaaa ttagaaattc gcacatcgat
     6661 cttatcacaa ggagcacagg agttgaatct gatcatcggt gtaccgaata atcaatatga
     6721 aacagtagtg aagggtatct atgaagggat ggtgaatacg gatgctgaaa gaatatcgaa
     6781 tggcactcca tcaaattcct gaaatcgggt ttcatgaatt caagacaaaa aaatatttat
     6841 atgaacaagt aaaaaacagc gaggccgtta tccatgaaat cggcgaaaca ggactactcc
     6901 tttattttga taatcagcaa gaagaaacga ttgcttttcg aacagatatc gatgcactgc
     6961 cagtcaaaga agaaacgaat ctctcttttg cttctaagca tgaaggttat atgcatgcat
     7021 gtggtcatga cggacatatg gcgatgcttt tagggttgac tgactttata actaaacaca
     7081 ttgaggatct tatttataat gtagtactga tctatcagcc atcagaagaa atttccggtg
     7141 gcgcacaaag cgttattcag tcaggattgt tggataaata tgaagtacaa gcaatatttg
     7201 gctttcatct atggcctgga ctagctaagg gagaagtttt ctctcgacca ggacctttga
     7261 tggcacaaag cagtgaaaca gacattatca ttaaaggcca gtctgcacat attgcttcta
     7321 gtgaaaaagg aatcgatagt ctggaggcag ctgtacgatt tttgaagaat gtttatgatt
     7381 ttgaccggca attaccggca gagtggaatc atctaatgaa atttggccaa atccaaggtg
     7441 gaacgatccg aaatgtttta gctggagaag ttattgtatc cggaagtatc cgttcttaca
     7501 gttcagatat gcaaaattat ttgaagaggg gattagacca tttagcagaa cagtttcact
     7561 ctacagctac tatttctttt cgctataatg acggctatcc agctgtaatc aaccatagag
     7621 gcttatatgc agcactgtta gattcaaaca aactacatga attagatgct ccagttcttc
     7681 aagccgaaga ttttggcata tatacgcaac aatatccatg tgtatttttc tttttaggtg
     7741 tcggtgatac ccctgcttta catgatccga cgttcgattt tgatatggat gttttagaaa
     7801 atggtgtaga atggtacaaa acgattcttt ttacagaaaa tcttcttgta aaaatcgata
     7861 gcagttcaaa agaggagcga gtaaataatg atgaatttat ccaatcgatt tagtcctagg
     7921 ctttctagaa tagaagtatc aaaaatacga caattcgatc agcaaatatc gtcgatacca
     7981 gatgtgatca aactcaccct gggagaaccg gattttccaa ctcctgaaca tgtcaagcaa
     8041 gcaggaatag cagcgattga agaagacttt tctcactata ccggaatgag aggattagag
     8101 gaactgcggg aagcggcttg tatatttcag caacagcgtt atggattaac ttatgatccg
     8161 caaacggaag ttttgacgac agttggtgca acggaagcga ttgcctctgc gttgttatct
     8221 gttttggaag aaggagacaa ggttcttatt ccagcgcctg cttattcagg atatcagcca
     8281 ttagtggaac tagctggtgc tgaattgatc ccgatagata cttctgacac agggtttgtc
     8341 tgtcagccgg aacaatttga acgagcattt gaacaatatg gatcagctgt caaagcagtc
     8401 attttaaact atcctaacaa tcctactggg acgacattgt ctgcaaaaca attgaaagca
     8461 atcgctgagg ttttaaaaaa atatccggta tttgttataa gtgatgaagt atatgctgaa
     8521 ttgacttata gtggaacgca tatgtccata gctacttatc ttccagaaca aacaatcgtg
     8581 ataagcgggt tatccaagtc acatgcgatg actggttggc gagtgggctt tatttttgct
     8641 caaaaagccc ttattgacga actaattaaa gtccatcaat atctggtgac tgccgctaca
     8701 acgatgtcgc aaaaagctgc ggctgaagcg ctaataaatg gggtgaaaga tagcgagaat
     8761 atgaaagagc aatatcaaac acgaagagac tatctggtcc agcaattggc gcctttagga
     8821 tttgaagtga cccagccaaa cggtgcattt tacttgtttt gcaaactccc agctgccatt
     8881 caaactgact cttggcagtt ttgtgtagaa ttagcggaac aggcaaaagt tgcgtgtatc
     8941 ccaggaattg catttggccc agaaggagaa ggttatatcc ggatttctta tgctagcagc
     9001 atggaaaaat tacatgaggc ctgcaaacgg atcaaagact ttttaacaga acagtgattc
     9061 atagatttta tcaaaaaaac aatcgttaat tttgcggtat attctgcaag gagacgattg
     9121 ttttttaaat ttagttcaaa tgaacgcgtt tcttttgatt cataactaat gtaatgttaa
     9181 gtttaaattg tttagaatga ttttaaaaga atattcaagg aggtttcatc aatggaaaaa
     9241 atcaaagttg tcattgtagg tgcatctcat ggagggcatc aatcgatctt ggaactattg
     9301 acacgatacg gtgaaaatgt agatatcacg ctatttgaag caggagacta tatttccttc
     9361 atgtcatgtg gtatggagct gtatttagaa gacaaggtaa cagatgtaaa tgatgtacgt
     9421 aatttcagac cagaaaactt tccgcaatcc aatgttcata ttttaaataa tcacgaagta
     9481 aaaacgatcg atgccgataa aaaaacagta acggttacta gcgcatcaga taacgaaaca
     9541 aaagaatatc cttatgacaa attgattttg agttctggtg tcaaaccaaa caatctgcca
     9601 gtaccaggag cagatctaga aaatgtctac ttgatgcgtg gatacaattg ggcaaccaaa
     9661 atcaaagaaa aattaaatga tccggctatt aaaaaaatcg ctgtcgtagg tgcgggttac
     9721 attgggatcg aagcagcaga agcaagccga aaagctggga aagaagtcgt attgcttgat
     9781 gtgatcgata gacctttagg cacttattta gatgctgaga tgacagatat tttagaacaa
     9841 cacctaaatg aaaacggtat tgaagttttg acaaatgcaa aaatcgaagc ttttactggt
     9901 aacggaaaag ttgaggcaat caaaacaagt gaaaaagaaa tcccagcaga tctagtcatt
     9961 caagctgccg gtgtgaaggc aaatacagaa tggttaaaag ggatcgtcga cttagatgaa
    10021 cgtggctgga tccaaacaaa tgaatacttg caaacgaatc ttccagatgt gtatgcagta
    10081 ggagatgcta cacttgctta ttctatccca gcacgcaaaa aaatgccgat cgctttagcg
    10141 actgttgcac gtagagaagc acgttatgta gtaaaacatc tttttgaaga agtaccaaac
    10201 aaaccgttcg gcggagtcgt aggttcctct gcattaagtg tctttgacta tcattttgca
    10261 gcaagcggat tgaatagctt tacagcagca aaagcaggag tagatgtacg agtgtcttat
    10321 tatgaagata cgttacgacc aaaatatgta ccagaagaac aaggaaatcc aaaagtgtcc
    10381 atccaattat tctcggatcc aatgacccat caattgctag gaggagcagt attatctacc
    10441 tatgatgtta caggtcaagg aaatgtattg gcactagcga tccaaaacaa actgacttta
    10501 gaagaactag cagaagctga cttcttcttc cagccaggct ttgaccgaca atggagcatg
    10561 ttgaatctag ccgctcaaca agcgttagga gaagaaccat ttgtagaata agaaaacgaa
    10621 atatttgaaa agaggttgtg aaaaagctgc cttgcatcaa gtaataagaa cagccaagta
    10681 aaaacagctt cttatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga
    10741 acaccgttta ttttcaaaaa agggactgtg acacgatttg tgtaccgacc cccaaaagtt
    10801 agaccaaaaa atctaacttt tggaggtcgg tattttttat ggttaaatat acttttgaac
    10861 ttaaattaaa gatcgttcat gattatttgg atggcaaagg cgattggacc gtaaaaatcc
    10921 agatcaagag attgaagcgg aaatgcttaa aattcgggag aagcacaagg attatggctg
    10981 tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa agaaagttca
    11041 gcgcttgatc aagaagctag gaataaaagt aacggcatac acgcgtaaat cacgtcgcta
    11101 caattcttat cgaggaaaag ttggagcagt agccaaaaat cggatccatc gtcgtttcta
    11161 caccagtatc tgccatcaga aaatcacgac ggatacaact gaatttaaat accatgaagt
    11221 ggatgccaaa ggcattatcc gtcaaaagaa attgtatcta gatcctttta tggatatgtt
    11281 caatagtgag attttatcct atcgtatctc tgaaaagccc aatgcattag cggtaatgga
    11341 aggtttggag gaagctatcc agaccactaa cgattgtccc tatcgtcgta cgtttcactc
    11401 agatcaaggt tgggcgtatc aaatgaaagc ttatagaaac aaattgaagg agtacaaaat
    11461 ctttcaaagc atgtctcgga aagggaactg cttagataat tcacttatgg agaatttttt
    11521 tggcttatta aaacaagaga tttttcacgg gaaagtttat aactgtttcg aggaattaaa
    11581 atcagccatc gatagctata tctattacta caataatgaa cggataaaac agaaattaaa
    11641 ctggcaaagt ctcgtccaat ttcgtaaaac aaccgtaacg gttgtgtaaa aatatcattt
    11701 tagagcataa aaaaaaggag tggatttctc cactccaaaa aagtctaact tttgggggtc
    11761 actacagtgt cacagtcctt tttttagtaa aaggatacat ttcttccttg tatattccat
    11821 gcgtgttcgt gagaaagcat atataattta attaacagat agttaggggg agaaaaaatg
    11881 attttttcaa ttacaatgaa actggtgttc gggttgatcg gtttattatt agtggtacgt
    11941 ttgcttggga agaaatccat gtcagaaatc acaccgtttg atttagtcta tacgcttgtt
    12001 ttaggtggga tcttagaaga atcaacctat gatgacaatg tccatgtagg gcatgtccta
    12061 tttgccattg cgctttgggc agtaatgatt tatggtatcg aacgtatcgt ccaaaaaaat
    12121 gaaaaggtca atcgttgggt caaaggcgag ccatctgttc taatcaaaga cggcgtcatc
    12181 aacatgacag agttgacaaa taaccatatc gagatggagc agttgcgagc aatcttaaga
    12241 cagcaagaat gcttttttct tgaaaatgcc aaacatgtta tcctagagaa tgccggtcaa
    12301 atgagtgtat tgaaaaaatc agatgaagac aaagctttat cgatcctgct tgtagatgaa
    12361 gggcaaattc agcataaagt gcttcagtcc aatcaattga ctgaagcatg gcttatggag
    12421 aacttgaaga aagaaggcta tgcagatgta aaacagctga tttatgttga atggtcagaa
    12481 gaaaaagggc tctacatact aaataaagaa gacgtcatgc atgaatctta ccgaatagat
    12541 ggatagatag acagaggttg tgaaatcagc gccttggctt gttactcaaa actaagcgac
    12601 tttttcacaa cctctgatat ctaatatacg ttgttccaga agttgcttct tcgaaaaata
    12661 agccaaaatt ccacaaaaat ttaagaagca attttcgaaa aatcctttct tattttttga
    12721 agttgatcac ttctgtcaca acctctcatg tcacaaacca tttttttgtt tcctttaact
    12781 caatataccg tacgtcttcc cacaatatcg gctgatggtc aatcataaag taatcttcat
    12841 aggcttcacc aatgaagtgc ccttcgacct catccaagag tcttccaaat tcatctttta
    12901 aattgagctg aatgattact gctttacgag ataaaaaggc ttctgtcagt atactatcga
    12961 tttcttgcaa tgtcatttgt tgtttaggaa gaggctgttt gcgtgcttct tcttcatttt
    13021 ttttgatacc gttgactagt tcttccatcg caaaagcagt cccccatttt aatccaaatg
    13081 gccgatctcg atactcgtta tagtcaatga atttttttgc tgtccgtctc atcttttcca
    13141 ccttctaatc cactcatacc acctgcatgc ccgccaacga gagaactacg tgcgatggcg
    13201 cgtcctcctt gcaagagact atgcgcatgg atgattgatt gaaagccgta tttttttcta
    13261 atcgtatcga caacaaaatc aactttcgct tcatttatct gcttttcagg atcttcaaaa
    13321 aggttaagct gtaaacctgt cgcgtatacg aggtcggaac agttcacgcc tacacgccga
    13381 atatcctggt agtggtaatg ctggtggaaa atggtcaata aagcatctgc tagatctctg
    13441 ctagcatttg tcgcttcgat tttcatttgc tggtgaaacc cggtcttgcc atttggatca
    13501 agatagccta aagagtatcc tacccataag ctgactactt gtgtttttgc tgcagatcgt
    13561 ctcaagcgag tgccgacttg gtcggccatt tctttgataa cgatctcgat ttcttctcgt
    13621 cttgtataat ctctatctag gatttgacta tttccgatac tttttgattt tggtgtatat
    13681 tcttgtccca aaaaacttcg atcgatgccc caagcatggg cataaagctg agtaccgatg
    13741 acccctagct tgcttttcaa gtgataataa tccgcgtgag ctaaatcata aacagaatgt
    13801 atacccattt gtttcagacg ttttgctgtt cgtttgccga ttccccaaaa gtcagtgata
    13861 tctgagattt tccataactt ttctggtaca tcttcataac gccattcagc aaacatttgc
    13921 tttccgtctt ttgagccatt gtctaaggct agtttagcaa gtagcgggtt atcgccaatt
    13981 cctatagtcg tgtagattcc ggtctgctca aacacatctt tatggatcag tttagccatg
    14041 tcgtacgcat ttgttttacc aaagagtgcc atgctatttg ttacatcaag gaagctttca
    14101 tcgacggaat agacatgatg gttcgcttcg tctgcatatt ttttatagat agaattgatt
    14161 tctctgttct tttccatata ataggccatc cttggggcaa caatatataa atcacttgga
    14221 taggggaagg gaagatctct ggcacggctg atattggtga tgccgtaagc tttttttgct
    14281 gcaggactgg aagccaagat taatccgcta ccccgtgttt ctgtcgaatc ggacggatag
    14341 gacatgacta ctaatttagt tgtaagggga tcaagatcct tttcaacgca ctctacggag
    14401 gcgtaaaaag atttgcagtc aatacataaa atatctcttg aaggttcttt ttcataaaca
    14461 aataccggat tttgattcgc ccacataaca atcacctctt caacacaatt agaacacacg
    14521 ttctctttcc gtgtcaataa aaaacagcaa ttttcaaata cgagcaaaag gctcacattt
    14581 ggaaattgct gagaggttat ttttatctat ttgtttttag ctgagatcgt tgattttttt
    14641 atcaaacatg aacagcttct ggatcgatct ttgttgtccc atttccaatg tgtgcaacat
    14701 aaaattccgg agcatagccg acaactttca tgtattcatc atagacattg tttttgaagg
    14761 caggtatatt ttcttctttg acaagagcaa tcgcacagcc accaaatcca gctcctgtca
    14821 ttcgtgcacc taagacacct tcttgttttt gagcagcatc tactagtgta tctaattcga
    14881 tcccagttac ttcataatca tagcgaagag agtggtgaga atcatttaac agttgaccaa
    14941 atgcttctaa gttcccagct tctaattcag cttttgcctt caaagtccgc tgattttcag
    15001 aaacggcatg gcgagctctg cgtatcaatg tatcatcgcc gatttggtca atagttgatt
    15061 caaattcttc ttcatctaag tctcctaaag aagagatagg caaagttttt tgcaaacgtg
    15121 ccaaggcttc ttcgcattcg ctacggcgtt cattgtattt tgaatctgcc aattcccgcc
    15181 gcttattcgt attcatgatg acgattgcat aaccatctag tttcactgga accatttcgt
    15241 atttcagcgt atttgtatct aataagattg catgatctag ctcaccgaag ccgatggcaa
    15301 actgatccat gatacctgaa ttgacaccga tgaattcatt ttctactttt ttacctgttt
    15361 ggactagttc caagcgatct gtttttaaat caaacagatc ttctaataca acacctacta
    15421 ataattctaa tgaagcgcta ctggaaagtc ctgcaccatt agggattgtt ccttccacta
    15481 ataactcaaa cccatgatcg attgagtgtc cagcttcttt taatttaagg accattcctt
    15541 ttacgtaatt tgcccagttt gcttttttat catattctag ctcatctaaa gtaaaactaa
    15601 tgacaccgat atcgtcaaag tttgtggaat aaactaatat tttattatcg tctcgaggtg
    15661 ctgctacacc ttgcgttcca taagtgatgg aagcggggaa gacatggcct ccattgtaat
    15721 ctgtgtgttc accaatcaaa ttgatgcgtc ctggtgagaa gtattgtgta gtttttttag
    15781 gtccgaatgt ttcggcaaat tttgtgttta agcttctttt gatttccatg atgagttatt
    15841 cctttcttgt tttgattttg aattctgtca ctgtttcaaa tactgatccg gcttttaaat
    15901 gaatcgaacc aaatgctgag aagcgctctg cacctggagc tgtttgagtc tcaaatgtga
    15961 tgccaccgtg atgtgccagt tttcggttgc gcatttctgg tgtttctgtg ccgaaatttg
    16021 cagtgaaaat aaccacacta gaagcatctg tagcaatatc tacttggatt cttttatcgg
    16081 ggctagtcaa gcgagcggct tctttaccca agccggtttc ttttaagaaa aatggatgat
    16141 cgattccgtc aacaagattt ttttgatcca aatcactagc aaaaacggac gctaattttt
    16201 taggtatttg aaaatcgaaa gcacttcctg cagcattttc ttttacccct ataggaatac
    16261 tatctgttcg caaaggagca taagcttcgc tattgagcca aagctcatgt tgatcgattg
    16321 actgagaagc atctcccgtc aagttgaagt aaacatgatt tgtcggattg aataaagtat
    16381 cttggtcact ggtgccgcga gaggtcactc gccagatatt atcttttgtc aaagtatagc
    16441 ggatttccac atccattgta cctggaaaac catgttctcc gtcgggactt gtagtagtaa
    16501 aaatgacaga agcttcattt tcgccattca aaatgacata agaccatttt tttaattcaa
    16561 atccaggagc accaccatgc agattatgtc cagtttttgg atctgtggct aattggtaag
    16621 tttttccatt gagagaaaag tggccatttt cgattctccc tgcggtgcga ccgatagaag
    16681 caccgatata cggatctttt tcgatatatt cttcagcggt gtcaaatccc aaaactaatt
    16741 ctcgatcatt tgattttaaa ctgacgattc tagcacctaa atcagagata gaaagtgcta
    16801 gcttgttttt gttctccaag gttatcaagt gataaccttg gccaaaatct ttttctgtaa
    16861 tcttcatttt caagatcctt tcttaatggg aaggaactac tgcatcgtga agttcatctt
    16921 ccattgtatc agctggagcc aatttgtttt ccaatttctc aacgatttgt gcatgttttt
    16981 tctctgtcaa tgtaatcttc caagcaaaaa taatagcgga gaaaatcatc aaagcagcag
    17041 gagcatagaa tgcataagct ttgaaagtcg ttacgccatg agcagtgata tcagcagcag
    17101 tagcattacc agtcatccca gaagcgatag cgacaaaccc tacgatcccg ttagacaaag
    17161 caccagcgat tttatctagt aaagggcgaa ttgatagagt aacagcttcg ttacggatac
    17221 catttttcca ttgaccatat tcaacagagt ctgtgatcgt catcaaagcg gataagaaaa
    17281 tcaattgttg agggaagtag aacaatacta gcccgatgat aacgattggc aaacttgaac
    17341 ctgcgatcgt aaagaaaaag taagaagaaa tcatcattgc gatccctcca aggtaaacgt
    17401 agcgacgaga gatgacgcgt gtaagcattg ggaatagtgg aacagctact aaaccaagta
    17461 aggcagcaac tagaccaacg atccaaaatt cagttggttg gccaagtaca tatttgaaga
    17521 agtagaataa tacggcagtt gttgctacat tggcaatcgc ataagcgata taagcaaggg
    17581 caagccacat caactgatca ttttgagcaa ctgctttgaa cacatctttg atcccagctt
    17641 tttcattttg ctgacggatc gcagtatcga cttcttttgt gcccaaggca gtgataatcg
    17701 cagtaccgcc tgaaataaca gcagcaatca caccgaacca gaaccagcct gaagcgcctt
    17761 caatatgttt atgagtaaca agataggtaa agaaagtaac gatagggaca acgactagag
    17821 tagtaccatt tgcacctaga gaagagccga aacgagcaaa cgtagcaaat ttttcacgtt
    17881 cctttgtttc cgtactcaaa gctggaatca ttgaccaaaa agcgatatcc ttaaatgaat
    17941 aaaaactatc caataagatg aaaacgattg caaataatat gataaataaa gtcgtattat
    18001 gagtagctaa gccaaagaaa ttggtaaaga ttgctaccaa gagtacggaa ctaacaacgc
    18061 cgccgataac taaccatggt ttgaatttac cgtattttgt acgagtatta tcgatgatgc
    18121 caccaatcaa aggatcaaaa ataatttcaa ctaagcggat acctacaact aagcttgtaa
    18181 ctgtagcaat catttttgca ttcgttgcct tatctgttcc tgcgaacatc tggcttgtca
    18241 cgaataccat aaaataagtg cttaatgtaa catagaatgt gtcgtgtcca aaagcgccta
    18301 aagcgtagga cacacgttgg atcattttgc ttttcataat ctgtattcct ctcctgttca
    18361 atatataata ctaagtaaac cggttacaac gaataacaaa gatcttttaa tgacttaaac
    18421 ttaacaagaa atcgcttcct ttttaatata tttctgtgac ttttatttaa ctttttgttg
    18481 cattatacta aaggagtaag gagggataga cgtggagcaa tcgatttttt taaaaaagga
    18541 acatcaacaa gtagaaaaag aattgtattt tgatttttgt ggtttctcta aaacgatgcc
    18601 gtttcattct tttggtcctg ctattcgcaa tgattacatc ctgcatgtcg tgatggaagg
    18661 caaaggatct tatcatgtga aaaatcaaca attccaattg aaaaaaggtg atctgttctt
    18721 gattcgtcca ggagactcga cgttttactt atcagatgga gaagatccgt ggatgtattg
    18781 ctggctttca tttggtggga gtgctgcaga agaaatcatt cggcagtcgc ttttcagaga
    18841 agaccaatac acgatggtct cttctgacat acaaaaatat atcgacatta ttttagaatg
    18901 catgaaagat acggaagaaa ccttggaaaa tgaattacag ttaacagcat taacctaccg
    18961 ttttttatca gtccttttaa aagatggcgg aaaggtcagc ggtggtgtac aaaaggttca
    19021 ttcctcttta gctgttgaaa cagtcaaata catcgaggca cattatgctg aaaaattgtc
    19081 tgttgaggaa atcgcccagc atttagcggt caatcggagc catttgtcgc gagtattcaa
    19141 aaatcatatg ggtaccagta tcaaggaata tttgatcggg gtacggatca atcgaggggc
    19201 gtttcttctt tctctgacgg atgaatccgt ggaaagtatt gcctatcaag tagggttcaa
    19261 tagtctagtc gtatttagcc gaatgttcaa aaagagtacc ggagaaacgg ctacgaacta
    19321 tcgtaaacgt atgaaaaacg aaaattttaa gaatattgct ttggatacct tgaaaaaaca
    19381 gttggaaaaa caaaccgcag tttcttggac aacctgatta gtctggttaa aatgacaagc
    19441 atcggcaaaa gaaacttgtg gcggttatgc tacactgtaa agcgaagact aaaaaggagg
    19501 aaaagggatg cgtacactta tcatcattag ccatccaacg atcgaagaat ctttaaatca
    19561 gcaatttttc aaagaagctt ctgctggttt accagctact tggcatcatt tggaatcaag
    19621 ttatccagac ggaaaaatag atatagagac tgaactacag ctcttggccc agcatgatcg
    19681 gatcattttc cagtttccgt tttactggta tagttctcct gctcatttaa aaatttggca
    19741 agataatgtc ttggaacgag cagcacatgt attaaaaggg aaagaactag gaatcgtatt
    19801 gacaacaggt gtggcggaaa aagaatacca agctggagga aaagaagaat atacgatttc
    19861 cgaatttttg aggccgtatc agcgaatcgc aaataaattc catatgacct atcttccgcc
    19921 attcgtttta gcacaattta tgtatttatc ccaggaaaaa cgttgggaga agctaattgc
    19981 ttatcagcag tatctctcat tggaaggcaa accatcgctg actcagcgga ttgactggtt
    20041 tattcaaaga gtacaggaaa atcagaaaat gcaggaagaa gaatctgaaa aacaagcata
    20101 tatcgtagaa gcactgactg aggcaaaaga gcagatcgaa gacttgacct ttacactgca
    20161 agaaatgaaa ggaacttcat tatgacaaat gattggctga tccaacaatt agatgaatta
    20221 agcgaaaaat cagaatatca agaccgtgcg ctcttatatg aaataaagaa actgatggct
    20281 gagatagacg aacgaacaat gcagctgcaa ggagaaatcg acggacaatc ttggaatcat
    20341 ctcaactggt gaaattttct tataaataac aaagataaaa atgtgcacag cgacaattat
    20401 ttgctacaat aagaatatga agcgtttgat aacgctatta tacataaaaa ttggtggtga
    20461 aaaaaatgta tgatgaaaat gagtggggag aaaatgttcg gatgacccga agaagacagc
    20521 aaaaacaagc agctcgtaag cggatgatca tgattggggc agcagttgcc gtgtttttga
    20581 tcatagcagg aggagttgcg ggtctatatt ttcatggaaa aaattcgtca gaagcgaaaa
    20641 gtacgaccat aacggaaaca acgacaaaga aaacagagac gaaacaaaca agtgagtcat
    20701 caactaaaac aaaagagaca caaacttctg aagaaaaaac agcaaatggt ttgccagagc
    20761 aacttcaagc aatcgtaaat gattattcga aaaagatcga agaaaagacg cctgcattga
    20821 ttgaagaata ccagacagaa attcagggaa atcaagaggg aatagctggt ttatcagcag
    20881 ttgctaacca aaaagcacgt gagcttcaag caatctccga cgaagggatc agaaaattac
    20941 gtgcagcgta tcaggcagca gaaaataaag acggtgtaga cttagatact ttgatcaatc
    21001 aattatcggc taattatacg aatcatgtcg ctaagatttc cgatatttat ttgcagacta
    21061 gcgcctctct tcaagcagag tcaacaagca cacaagacac aacatcttct gattctgaga
    21121 cagcagagtc ggtagaagct tctgaaatgc aagctcgaac aacacaagac acaacagatt
    21181 cctctgaaac taataccaac agcgcgactt cacaggctgc gacaaccgta gttttggaag
    21241 gagaaggtcc aaatcaaata gccgaacgaa ctggcgtacc agtagaaacg atcttgtcgc
    21301 tgaatgggat gagcatggag aattatttcc tgactcccgg tgagacgcta aaattgaatt
    21361 gataaaagca ataaaaaaga acgaagcttc cactaaggtg aagcttcgtt ctttttttat
    21421 tttccggttc ttttttcgcg tacaaagtaa tggatcgtca tgtaaagcaa gaaaacgagt
    21481 ccaataatca atgagattct cgtttcagga ttgataaaca tgaaaatcaa tacgatagct
    21541 aaaaaagcaa gtgtcacata attcgtataa ggagatagtg gcattttaaa aggatgatca
    21601 tgcattttat ctgcgtgctt ctttttgaag cgaatctcgc tgatcaaaat cactacccaa
    21661 ggaaccatac caggcaaaat acttgagctg tatacgaaaa cgaaaatatt ttctgactgg
    21721 ttgatgaaca ttggtagaag ataatttaag atcagaccaa ttaagattcc acctgagatt
    21781 gctacgacag gataaaaagg cgcaccgtga cgagatagtt tcagaaattt tttagacatc
    21841 tttccattta tggcaagtgt atagaccata cgacttgcgc tgaaaatccc tgaattgcaa
    21901 cctgataatg ccgctgtaat cacgacaaaa ttaatcagtc ctgcagcaaa tgtaatgccg
    21961 actttagaaa aagtttgtac aaaaggtgaa ccaatctcac tcaattgatt ccacggatag
    22021 atactcacaa tgataaaaat agcaccaata taaaaaatca agattcgacc gatagtggac
    22081 tggacagctt caatgatcgt ctttttagga ttctctgctt caccagcagt cattccgatc
    22141 agttcgattc cttgatagga agcaacaacg atcgataatg caaagaaaaa tcctttgacg
    22201 cctccagtga agaatccacc atttttccaa agatttgaaa tccctactgc ctgaccatga
    22261 ttaccaaatc caaataagat taccccaaga cctgcaatga tcatcaaaat gatcgttatg
    22321 actttgatga tcgaaaacca aaattctaat tcgccaaacg ccttgacaga aataagattt
    22381 gccagcataa gaaacaagac gacaatgatt ccgggaataa ttgccggcaa atcaggccac
    22441 caataattca tataagatcc tacagcaatc acttcactta ttcctacgac aatatattga
    22501 aaaatattac tccaagcggt caagtagcca gccacaggat ggatgtactc gctcgcgtaa
    22561 tttgcaaaag aaccagtcga aggatcaacg taaagcattt cgcctaaagc acgcataatc
    22621 aaatacaaaa agaatcctgc aatcgcatag gctcccatca ctgagggccc tgtccaacgt
    22681 atggttgagg aggcacccat aaacagaccg acaccgatcg ttccacctag tgcgatcatt
    22741 tggatatgac gggcactaag acttcgagtt aacttttctt cttccataca aaaactcctt
    22801 tcgttgttga gtcaacaaga aaaaataatc aaacgtttca acacatgttt ttcattatta
    22861 ggttagcctt attgaactct tgatgtgatt ctacagaaaa ttttcataaa atgcgaggaa
    22921 aattcgaaaa aaactttttc atcattctac ttgaaaatgc tttccatttt agttaaacta
    22981 tatttaaata aaaagttagg taaacctaaa aaggagtgta agatttggaa cgtacggcca
    23041 ttgtaattca agacctttca gccgcttatc aagggaaaac tgttttgaaa actatcaact
    23101 tgacgataaa tccacaaaaa attaccggta ttatcggacc aaacggtgca ggaaaatcaa
    23161 catttatgaa atctttgcta gagttgatac cagctactac tggtaaagtc accttttctg
    23221 gacagtcggt aaactcagtc cgtaaaaaaa tagcctacgt ggaacagcga agtgaattgg
    23281 atctttcctt tccaattgat gtattaggcg ttgtactttt aggaacatat ccatctttac
    23341 gaattggaca aagacctggg aaaaaagaaa aagaacgtgc aagacaagct ttgaaaaaag
    23401 tagggatgga agaatatgcc aaaagacaga tcagcgaact atcgggtgga cagcttcaga
    23461 gagtttttat tgcaagagct ctagcccaag gagcagaatg gatcttttta gatgaaccat
    23521 tcgtagggat tgatgcgtta agtgaacgaa agatctttga catcttgcag gaattgaaga
    23581 attcaggaaa aacgattttg atcgtccatc atgatcttca taaagtagaa gaatatttcg
    23641 atgaggttat tcttttaaat aaacagctga tcgcttccgg tccagtacaa gaagtcttta
    23701 catcgaagaa ccttcaattg gcctatggtg agatcatcag acatttagta aaaggaggag
    23761 agaaaaaatg atccaatcct ttattgatgg attaatgaat tatcaattcc tgcaatacgc
    23821 tttggtcact tccatgcttg taggacttgc ttctggagtc atcggttctt ttattatttt
    23881 acgagggatg tctttgatgg gagacgctat ctctcacgca gttcttccag gtgtagccat
    23941 ttcttatatg tttggtttta gttacatctt tggagccact gcttttggga tgctaacagc
    24001 agcagtgatt ggtttcgtga ctcagcggag ccgattgaaa aatgatacag cgattggaat
    24061 tgtctttagc tccttttttg cattagggat tatcttgatt tcatatgccc agagtgcgac
    24121 agatttgtat catattttat ttgggaatgt cttagctgtt agggaaagcg atttgttgct
    24181 gacagcactc gttagtggaa tcgtcttgat atttgtcttt ttcttttata aagaattgaa
    24241 gatcacatca ttcgatccga caatggcaaa agcttatggg ctgaacacat cgctgatcca
    24301 ttatcttttg atgttctttt tgacattagt ggctgtagtc agtttacaga cagtaggaac
    24361 aatcttggtg attgccatgt tgatcacacc agccgccacg gcttacttgc taacgaacca
    24421 tttactgaaa atgatcatta cagctgcagg aatcggtatg ctaagtgcag ttgtcggtgt
    24481 gtttttcagt tatagttaca attggccatc aggagctacg atcgtgttag catgtaccgc
    24541 attttttatc cttgcttttt tattttctcc aacaaaagga attttatttc agaaaggaag
    24601 cagatagatg aaaacaaaaa aatcactctt tttaatcttg gcagttagtt tcttggtatt
    24661 agccggttgc ggaaagcagg cttctgacca agcggatgaa ggaagtaaag aaaagttgtc
    24721 agtcgtggct accaattcga tcttggcgga catggcaaaa gaggtcggaa cagatcaaat
    24781 agatatccac agtatcgtac cagtcggaac agatccgcat gaatatgaag tactaccaga
    24841 agacatcaaa aaggcaagcg atgcagatgt tattttatac aacggtttga atcttgaaac
    24901 aggtaacagc tggttcgata acttgatgga aacggctaaa aaagaagaag gaaaagatta
    24961 ttttgcagtt agcaaaaatg tagaacctct ttatttaact agcggtgaag aacatacaaa
    25021 agcagatccc catgcttggc tagacctatc taacggaata aaatatgtgg aggaaatcgc
    25081 acgtatattc tctgaaaaag atgcagaaaa tgcgacactc tataaaaaaa atgcagaagc
    25141 atatgtggaa aaactaaaag aattagatac acaagcgaag gaaagttttg cttctatcga
    25201 agagaacaaa aaattattag taacaagtga aggggcattc aagtattttt cacgagcata
    25261 tgatttgcca gcagcttata tatgggagat caatacagaa agtcaaggta cgcctgatca
    25321 aatgaaagcg atcattgatc agataagagc aaaagaagta ccggttttat tcgtggaaac
    25381 cagtgtagac tcaagaagca tggaacgggt agcaaaagaa acgggtttga aaatctatga
    25441 taaactgttc actgattcca tagcaaaaga aggagaacaa ggagattctt actatcagat
    25501 gatgaaatgg aatattgaaa cgatccatga aggtttgtca caaacaaaag aaagctgaaa
    25561 agtgcaaggt ctagcttgag gagcagacct tgcatttttt ataagagata tgaaaaaaaa
    25621 ttatgctgtt tttcgtcgta cagatacctt ttttgaagaa attccgttat agttagagta
    25681 aggaaggaag gtgtggctat gaattttaca cgtaaagata aatatacgat taaagaagaa
    25741 gtctttcgat tgatcagcca gcaggaaaat accgacaagg atcaagacct tcaatttctt
    25801 gaagcagaat tagctaaatt attcatagca aatcaaaatg agacaacaga accagatgaa
    25861 gagctgtaca taggcaaatg gttgtttgat gaatttcagc ctttagatga aaataccggt
    25921 atcggttatt ttgtgttcct ttctccagtc ggcttcacac ctggcgtatt agttaaacaa
    25981 aacgacgaaa taaccgtcaa agcatggcct gcattatttt cagtcatcaa agcagaagga
    26041 gtaatcaaaa agatccagtc ggcctattat aaaagtcaat ttattgccaa cagcaaatta
    26101 atgaatatgt tttataatcg tctagtagaa attgctaggc ctgggaaaat cccgcaaaca
    26161 ttcacgtaca cacagcaaaa aaatctctat gaagaagtat tgtattcccg cgatctgtct
    26221 gagttttcgc catgggaaga ggtaaggctc catgaactgt taagtacaaa accagtttca
    26281 ttgaattttt ttaatgcatt agataacaaa agaaaattaa tttcaacaaa ttattgggaa
    26341 gtatttgctg caagaaaaaa atggatggaa gaaaagaaga ttgccgatca gcagatccag
    26401 tttgtccagc tgaaaaaaga aaaactatat tatgggttat acagccgtta acagcaagcg
    26461 aggaagacca tgacattaaa agaagaaatc atacaagaaa gcaaacgatt aggaattgac
    26521 aaaatcgggt tcaccacggc ggaacctttt gattcattaa aagaatcgtt ggaagaacaa
    26581 aaagcagctg gccatacttc tggctttgaa catccaaata tcgatgagcg tctttatcca
    26641 gaaatgacat ttgatcagcc gaaatcgatc atttccattg cacttgcgta tcctactagg
    26701 atccatgaag aagtgcctag ggatgaaaaa cgcgggcagt ttgctcgtgc ttcttggggg
    26761 atcgattacc atgatatact cagagaccgt ttgaaccgat tgatcgaatt catccaaaca
    26821 agagcagaaa aatggcaaaa agaagaggaa tggcgtctag ctccgcaagt agatactgga
    26881 gaactgatcg atgtcgctgt agcacagcga gctggtttag gatttattgg acgaaacgga
    26941 ttgttgatca ctgaagagtt cggttcattt gtctatcttg gtgagatcgt tactaatatt
    27001 gtatttgagc ctgataaacc aggagaattt ggttgcggtg actgtatgcg ctgcgtgact
    27061 gcttgtccga caaaagcatt gcttggtgac ggaagaatga atgcccagcg atgtttgtct
    27121 tatcaaactc agacaaaagg gatgatgccg gaagaatatc gtaaaaaaat gcgaaatgtc
    27181 atttatggat gtgatatctg ccagttagtc tgcccttaca atcaaggaaa agattttcat
    27241 tttcatgaag agatggaacc aaaagtagac gaaatatatc caaaattaaa gccaatgctt
    27301 tcgatgtcta ataaagactt caaacagcaa tttgggcatt tagctggttc atggcgtggg
    27361 aagaaacctt tgcagagaaa cgcattgatt gctttagcta atctaggtga tcgaacggca
    27421 ttgcctgaga tttcagaatg tttaacagat gtccgccctg tcatcagagg aacagcagct
    27481 tgggctattg gacgtctagg aacaaaagaa ccggaaaaat ggcttgatgt attagatgaa
    27541 atattagcta aagaaacaga agaagtcgtt ataaacgaat tggaacatgc ccagaaatta
    27601 ttgcggagaa agttaaaata aaaaaatagt taacattatt caaagttttt gtcca
//
LOCUS       DAFCLG010000025        25798 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFCLG010000025 DAFCLG010000000
VERSION     DAFCLG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.025"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            136..2196
                     /locus_tag="LND56_001029"
     CDS             136..2196
                     /locus_tag="LND56_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBK6742969.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2279..2848
                     /locus_tag="LND56_001030"
     CDS             2279..2848
                     /locus_tag="LND56_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6742970.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3023..3829)
                     /locus_tag="LND56_001031"
     CDS             complement(3023..3829)
                     /locus_tag="LND56_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6742971.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3967..5481
                     /locus_tag="LND56_001032"
     CDS             3967..5481
                     /locus_tag="LND56_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBK6742972.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5884..6867)
                     /locus_tag="LND56_001033"
     CDS             complement(5884..6867)
                     /locus_tag="LND56_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBK6742973.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6872..7519)
                     /locus_tag="LND56_001034"
     CDS             complement(6872..7519)
                     /locus_tag="LND56_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6742974.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7604..8236)
                     /locus_tag="LND56_001035"
     CDS             complement(7604..8236)
                     /locus_tag="LND56_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6742975.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8252..8803)
                     /gene="coaC"
                     /locus_tag="LND56_001036"
     CDS             complement(8252..8803)
                     /gene="coaC"
                     /locus_tag="LND56_001036"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBK6742976.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8800..9537)
                     /locus_tag="LND56_001037"
     CDS             complement(8800..9537)
                     /locus_tag="LND56_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBK6742977.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9534..10523)
                     /locus_tag="LND56_001038"
     CDS             complement(9534..10523)
                     /locus_tag="LND56_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK6742978.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10781..11323
                     /locus_tag="LND56_001039"
     CDS             10781..11323
                     /locus_tag="LND56_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6742979.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11340..12425
                     /locus_tag="LND56_001040"
     CDS             11340..12425
                     /locus_tag="LND56_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6742980.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12437..13243
                     /locus_tag="LND56_001041"
     CDS             12437..13243
                     /locus_tag="LND56_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742981.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13244..14071
                     /locus_tag="LND56_001042"
     CDS             13244..14071
                     /locus_tag="LND56_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6742982.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14068..15141
                     /locus_tag="LND56_001043"
     CDS             14068..15141
                     /locus_tag="LND56_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6742983.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15569..18265
                     /locus_tag="LND56_001044"
     CDS             15569..18265
                     /locus_tag="LND56_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6742984.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18342..18830
                     /locus_tag="LND56_001045"
     CDS             18342..18830
                     /locus_tag="LND56_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6742985.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18842..19336
                     /locus_tag="LND56_001046"
     CDS             18842..19336
                     /locus_tag="LND56_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6742986.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19357..20166
                     /locus_tag="LND56_001047"
     CDS             19357..20166
                     /locus_tag="LND56_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6742987.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20163..20984
                     /locus_tag="LND56_001048"
     CDS             20163..20984
                     /locus_tag="LND56_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6742988.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21035..23134
                     /locus_tag="LND56_001049"
     CDS             21035..23134
                     /locus_tag="LND56_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBK6742989.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23131..24249
                     /locus_tag="LND56_001050"
     CDS             23131..24249
                     /locus_tag="LND56_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK6742990.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24288..25166
                     /locus_tag="LND56_001051"
     CDS             24288..25166
                     /locus_tag="LND56_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6742991.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25357..25509
                     /locus_tag="LND56_001052"
     CDS             25357..25509
                     /locus_tag="LND56_001052"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6742992.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8160 a         4478 c         5439 g         7721 t
ORIGIN      
        1 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
       61 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
      121 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
      181 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
      241 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
      301 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
      361 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
      421 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
      481 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
      541 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
      601 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
      661 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
      721 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
      781 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
      841 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
      901 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
      961 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     1021 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     1081 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     1141 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     1201 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     1261 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     1321 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     1381 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     1441 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     1501 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     1561 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     1621 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     1681 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     1741 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     1801 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     1861 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     1921 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     1981 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     2041 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     2101 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     2161 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     2221 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     2281 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     2341 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     2401 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     2461 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     2521 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     2581 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     2641 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     2701 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     2761 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     2821 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     2881 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     2941 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     3001 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     3061 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     3121 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     3181 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     3241 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     3301 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     3361 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     3421 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     3481 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     3541 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     3601 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     3661 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     3721 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     3781 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     3841 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     3901 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     3961 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     4021 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     4081 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     4141 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     4201 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     4261 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     4321 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     4381 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     4441 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     4501 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     4561 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     4621 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     4681 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     4741 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     4801 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     4861 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     4921 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     4981 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     5041 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
     5101 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
     5161 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
     5221 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
     5281 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
     5341 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
     5401 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
     5461 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
     5521 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
     5581 ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
     5641 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga aaaagagcgt
     5701 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     5761 gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
     5821 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt gatcagcatg
     5881 attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt ccatatattc
     5941 atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc gaatcttgcc
     6001 attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg ctttatactg
     6061 atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa tcaatacacc
     6121 aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc aacgtccatc
     6181 aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat ccaaaataac
     6241 atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt gttttttata
     6301 gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt ctgatcctgc
     6361 agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg ctgttcccac
     6421 accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc caagccagta
     6481 aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa catagtctag
     6541 cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg cataaccacc
     6601 tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat cacttttatt
     6661 ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt tcaaagagac
     6721 tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt tgatcagtaa
     6781 ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat ctacagaacc
     6841 aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat tcttggaatg
     6901 ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa atatccgttt
     6961 cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct
     7021 tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc aatgtgactg
     7081 gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca taatgtttag
     7141 taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca taaacagcac
     7201 tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt ttgattgcgc
     7261 catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa atcaatacat
     7321 cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca aaagagacat
     7381 acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc cctgcaaaaa
     7441 tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac
     7501 ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc attcaatagt
     7561 aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta gtaagagaag
     7621 ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga cctgcgacga
     7681 ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa acagcattta
     7741 aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc atattcatca
     7801 ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa acggaatctg
     7861 ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc accccaatca
     7921 agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct gttccgatga
     7981 ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg ctacggatca
     8041 tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct aaaacgatcg
     8101 aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata gggataaaac
     8161 ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt
     8221 tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta agagtcagca
     8281 tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa tctccacaag
     8341 ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat gtttccagat
     8401 tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca atcaattttg
     8461 gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca atgccgtttg
     8521 ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca gcttgttttg
     8581 ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct gtatggactg
     8641 cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg cttttagaca
     8701 taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca gcgcttttgt
     8761 aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt cctccagtct
     8821 aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt ccactgttcc
     8881 atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc
     8941 gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca gaagagattc
     9001 ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct gccattcacg
     9061 tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct cggtgttaga
     9121 agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat tgtccagttc
     9181 ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa aactaaaagc
     9241 aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt cttttttcgt
     9301 tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa tcgttatatt
     9361 ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg tggtatccgg
     9421 attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat
     9481 agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa tcttcattta
     9541 tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag tttagatgct
     9601 gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc aatctgcaaa
     9661 tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac agaatcaatt
     9721 ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag tttgattccc
     9781 ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag aagactgctt
     9841 agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc taaagctttt
     9901 gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag actttttagc
     9961 catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc tagtttatgt
    10021 aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac aactttttta
    10081 tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt aaaccctgct
    10141 gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac taaccagtct
    10201 cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag cccaaaacca
    10261 gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac gacagttccg
    10321 gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga ctcgctcatt
    10381 gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac ttcattttca
    10441 gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc ctttattacc
    10501 tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc ttttatcata
    10561 ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa
    10621 aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc agcaaaaaaa
    10681 attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt ggtagtaaac
    10741 aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg gtgagaagtt
    10801 aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac gcacagattt
    10861 gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa tggaaacatt
    10921 cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc aagaagcagc
    10981 gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg aagaaaacgg
    11041 gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct
    11101 gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg aaacgtttat
    11161 ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg tggcaaaagc
    11221 aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa
    11281 acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg ggtaaaaacg
    11341 tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat gaaacagtat
    11401 taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt ggtccttccg
    11461 gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct acagaagggg
    11521 acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga caagtaaata
    11581 cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac gttgcttttg
    11641 gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc
    11701 gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa
    11761 gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg ctattagatg
    11821 aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa ctgcgggatc
    11881 tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa gaagcgttgg
    11941 caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt ggtacacccg
    12001 tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg gagagcaata
    12061 tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag caatttgaat
    12121 gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc ccagaggatt
    12181 tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc
    12241 gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg atggtccatt
    12301 caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca gaagatatcc
    12361 atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac agctacgaag
    12421 aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt atattttgtg
    12481 gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt tttttgatat
    12541 gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga catcgctttc
    12601 tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc tgatcatcag
    12661 ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc tgatgctgat
    12721 tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg ggattttcag
    12781 tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga aacagatatt
    12841 attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat ttatgattat
    12901 gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta gtagagattt
    12961 aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat taaatggtgt
    13021 gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc tgactcgtct
    13081 gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt tcttggttac
    13141 acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta tgttcatcgt
    13201 gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg
    13261 gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct tctatctgat
    13321 tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta cttgggaaaa
    13381 ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata cgttcatgct
    13441 ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta tgggaatcta
    13501 ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca tcttattagt
    13561 ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac taggattcgg
    13621 attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa tcgtcgtctt
    13681 gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct
    13741 tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt ctccaggaat
    13801 catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg ttacattttt
    13861 cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg caagacaagg
    13921 aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca tgatattggt
    13981 cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag
    14041 agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt tttaggtatt
    14101 ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg
    14161 agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc tagtttgatc
    14221 acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga ttctaatgaa
    14281 gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat accaagtgaa
    14341 tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca ttcgaagatc
    14401 aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc agataatcag
    14461 tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa attctttgat
    14521 gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga cagtttgatg
    14581 ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg ctattctttg
    14641 aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca tttaacacca
    14701 aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga agaagcctcc
    14761 gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga acatttacat
    14821 tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat gccaaaaacc
    14881 gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc ggaaaatgcg
    14941 gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa agcgttattg
    15001 ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat ctcccatctt
    15061 gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct attcttagaa
    15121 ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta tcaacgttct
    15181 aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc aaaagcggat
    15241 gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta ccagagaaaa
    15301 gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa gattgaaaaa
    15361 gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt ctgtaatcta
    15421 ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa acataaaaat
    15481 catattagat aaaaactaaa ttggtagata aattattttg tcagatatct gaggcaaata
    15541 ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa tatagaatag acatattgca
    15601 gccgatagtt tattatgctt ttagacacat taaactggag gaaaaaatga aaaagagcgc
    15661 taaagatcga gtttttgatg agctgatacg actaacaggg aatatgtctt ctgtcactgc
    15721 cgagaaaatt tcgaagaatc taggtatctc tcgtcagaat gccagccatt atttgacgag
    15781 attagtggaa gataaaaagg tcgagaaatt accgggaaag cctgttttgt ggaagccttt
    15841 agatgaatat gcggttgtag ataatacgga gcaaataaac gaagcctttc attctgtagt
    15901 tggtcatgac ggaagtctga gagaggtcat tcaaaaatgt atagccgctg tcaaatatcc
    15961 gcctaacgga ctcagtgtat taataaatgg agccacgggt gttggaaaaa gttttttagc
    16021 cacaaaaatt tttgaatacg ctatccatga gcaaattatt gaaaaagatg caccatttgc
    16081 tatcttaaac tgtgccgatt atgcggacaa tcccgaactg ctttcggcaa cactgtttgg
    16141 atacaagaag ggggccttta cgggggctga aaaggatacc gatggtttat tggcaacggc
    16201 caacaacggc tatctttttc tcgatgaggt ccatcgccta tcgaaagaaa atcaagaaaa
    16261 gctctttcta tttattgata ctggaaacta tcgtccagtc ggtgaaaatg taaattggca
    16321 ttctgctaag gttcgtttta ttttcgccac gacggaaaaa ggtgaaaatt atttattaga
    16381 cacctttgat cggcgtattc aaattagtgt tatgttgcca acttttgaag atcgccccat
    16441 tcgagaacga ttagagctga ttcaattatt ttttcaaaat gaagcggatg ttttacaaaa
    16501 agatattatt gtttcaagag aagccttgtc tttaatgtta tctcatccct ttagtgggaa
    16561 tattggtaag ctgaagaata tcattaaaat cagttgtgcg gatgtgtata gtcgaacaca
    16621 agaagaacca ttaacgattg ggaaaaatga gatcttgatt cagttaaata tgctggaatc
    16681 cgaagtagaa agcttgccga ttgctagttt attgataaag aaaaatcaaa agataaaaac
    16741 ggatcgaatc aaaccgtttg attatgattc gattttggaa aaaattactc agctgataaa
    16801 tgaaggtaat ttcaatgaaa atttttcttc gataaagaat gaagtacagc atttggtgca
    16861 tcacattgat aaggattctt ttttatcaat gagtagaaaa ttgatgattc agatgtttag
    16921 aaaggtctgg gtatccgttt taggaaaaac gtacggattg acgacgagtg gacctgttgc
    16981 agaaattatt agcagatcct atgtcatttc cccaagctat aagacagact taaccgggat
    17041 aaatcgtaca ttagctgcaa atctacctcg gacgacttac ttgtgtaatc agttcttgga
    17101 gcatttacca ccgctctcag aaaaggacga gaactttcta agactgctta ttgtcgtgac
    17161 tttatctgat tatgtagatg aatccatcga gctgaagggc ctgttgcttg ctcacggaga
    17221 tagtacggct agcagcattc aagcagtagt gaatcaatta tgcggcaatt atgtttttga
    17281 agcgttggat atgccagtag agagtaatgt gtcagagatt gtcgaatcga caaggaattt
    17341 cgtgaaacgg caagtgaata cagatagtct agtgctgatc gtagatatgg gctctttaaa
    17401 tcaaatttac acacagatca agaacgattt aaaaggggag ttacttcttc tgaataattt
    17461 gacgacttcc attgcacttg atattggctt gaagatgacg atgaatgcgc catttaaaca
    17521 gattgcggag gatgcagcag acaagtattc aatcaatgtt caatactttg aaggcttttc
    17581 tcaaagtagc aacattatca tttcctgtat gtcgggtttg gggatttcgg acaaattgaa
    17641 agatgttttt cgtcatcatt tatcggatga atccatcgaa gttttcacga aggactacag
    17701 agaactacgt gaattaatcg accaaaatga tgaaacttat tttgacaaga caaaattggt
    17761 catcacgact tcggatcttc cacgttcctt ttcaattcct aatattaatg tttatgacat
    17821 cttagacggt gatggagcag ccaatttgga gcatattttg agtcctgaaa tgaagccggc
    17881 agaatttaac cgcttgattc aggagctagt aaaattcttt tcgttggaag gaattgcgga
    17941 ccgtttgaac tttctgaacc caacgatcgt gatgagtgaa gtggaaacag tcatttctaa
    18001 atatgaaaat tactatcatt tcactttaaa tggccgtgta aaactaaatc tctatatgca
    18061 tacggcattg atgatggaac gtttattctt ggctcggaca gaaaaatctg aagaaccgaa
    18121 ttatgatttg aaaccagatg aagatgaatt ttattatgtt tctagaagta tttttcaatc
    18181 aatggagatg aaatataatt ttaaagtcaa tgcttatgag ctttcgctgc tctatgaatt
    18241 attaggaccg tatattcaaa aataaagtgt ctaaaaaagt gtctaatctg ttttttttag
    18301 gttagacact ttttttaatg tgaattaacg tgattaaacc aatgttttta aaagttggca
    18361 caggaattgc ttatataaaa gtgaacagaa agaaaggaat taaccatatg aagaaaattc
    18421 taatagcgac tcacggtcac ttagcttcag gctttcttag cagtattcaa ttactgacgg
    18481 ggaaggttca agagattacg gtgattaatg cttatgtgga tgagtgtgat tttgaacagg
    18541 agttgaatgt atttgtaaac caggtgaacg agcaggatca gatttttgtt tttaccgatt
    18601 tatttggcgg aagtgtcaat cagaagatta ccaaaacctt tttggagaaa gagattgctg
    18661 taactatttt gtctggcttt aatctgccaa ttctgctcga aatagtattg gcaaccaagg
    18721 aattaacaaa ggaggatatt caggaactgg taaataaatg ccaacaggaa ttaaagatga
    18781 ccattcttga tgaggtacat gtagtaaatg aagaggaaga ttttttttag gaggaagaaa
    18841 aatgattaaa gcagtgcgaa ttgatgaacg attaattcat ggacaagtag cgatgacttg
    18901 gacaaagagt ctaaatttaa ccgggttagt agtagcaagc gatgaggcgg ctaccaacga
    18961 tattcaaaag atgacattga agatggctgc accatcgaat gtcaaagtca ttattaaaac
    19021 agttgatgac gcgattcatt tgttaaaaga tccgcgggcg aaggacatgc gcttgatggt
    19081 tttagtccct aatgtcaaag atgcggtaaa agttgcgaaa gctttcccaa cggaaatcga
    19141 attaatgaac gtggggaatg ctgggaaaat gacaccgcca tcggaagata agaaaacctt
    19201 atcaaaggaa gtgatgttaa cgacgtcaga aattcaagac ttaaaagaat tagttgaact
    19261 ttatccagat accgtttttc agcctacacc ggcgatggaa aaacgttcag ctatctctat
    19321 tttgagtgat ttttagaaaa aggaaggaga aaaattatgt atgatgcatt tgtagtcgct
    19381 ttggccgttt ttattggagt tgctggacat gaaatgttcg ggatgtccat gctgagtcgt
    19441 ccgattgttg ttgctccgtt agcaggagcg ttgatggggg atttgcagac ggggttgatt
    19501 gtcggggcgt cactagaagc catctttatg ggtgtcgtca atatcggaat ctctagtacg
    19561 gcggagcctt ctttggctgc tgggctggct acagcgttcg caatcaaaat gggaggaaac
    19621 gtggatgcga ttattcccat tgtctttcca ctagcagtat taggattaca gttaatgaat
    19681 atgatttttt cttttgtatg tggaccgatg gctgctaaat ttttcaaatt tgcaaaatct
    19741 ggtgatcaaa aaggaatcat caatctgcac ttctttatgt ggtttgtcca ctatgcgctt
    19801 tatgctctta ttccattctt tgccgtttta tttggagcag atgttgtcaa aagcgtcttg
    19861 gataatattc cagaggtcat catgaacgga ttaacagtcg ctggaaatct gctgccggca
    19921 gttgggatgg cgatgctgtt acggatgctt tggagtaaaa atattgctgt gtggtttttc
    19981 tttggggccg ttgttatggc gtatctcgag ttaccattga ttgcaattgc cgcagttgga
    20041 acgattattg caattgttat agcccaaaga gatctacaac taaaaaatat atctgttcat
    20101 gcagttcaaa gcgatgctgc tgtaaatgca acgcttgacc aagaagagga ggatttcttt
    20161 caatgaaatt agctgatgga atttctaaag atgagaaaaa attggttcgg aagatgtttt
    20221 ggcgctcgat gactatgtat gcttcggtga atccggtgac gatgggcggc ggtggtttcg
    20281 cctattctat gatgcccttc attaatcatt tctacaaaag tgaagaagat cgagctgcgg
    20341 cgctggaacg tcatacacag tatttttcga cgaccattcc ttgcgcgtcc tttgtcatgg
    20401 ggattgcggc atcgatggag aaggaaaatt ctgaaaaagc tggttttgac gtggaaagta
    20461 tcaacgcgat caaaacgagt ttgatgggac cattggctgg aattggcgat tcattgttct
    20521 ggggagtttg gcgtgtcgtt accgcgggac tagcgatcaa cttagccaat gccggaaata
    20581 ttttggcacc aattatcttt ttagttatgt tcaatatacc taattacttg atgcgatact
    20641 atggcggcgt tttaggctat tcattaggct ctaaatatat tgaaaagctg tatgcgaacg
    20701 ggttaattga aattctgaca aaagctgcta gtatcttggg tctgatcatg gtaggtgcaa
    20761 tgacgatcag tatggtcaaa ttcaataccg ttttgagctt ttcaatgaag ggcgaagaac
    20821 taatgaatgt ccaagaaatc ctcgatagta tttttaaagg aattgtaccg ttattggtaa
    20881 cattgagctg tttcaaatta ctagataaaa aagtcggcgt catcaaaatt atttttggaa
    20941 tggtcgtctt gggaatttta ttgtcattac ttggaatcgc atagtaggaa gctgcagcta
    21001 ctaggctgca gtttttttag caaggaggga aaagatgact attaatgaag taatttattc
    21061 acccaaacac ccatttttta atgctgagtt aaacgacgga aatgtgacta cttcaaacga
    21121 atacagctac tatcggaata ttcctgatgt aaagggattg accttcgatg taagtaatca
    21181 aaagacatta gcaaaccttg atgtttgcgg gacgattcaa acgttgacct tttatcgaga
    21241 aaatttacta actgaggaaa aaccaggtgt ttgggtcaat aaacagctct ctcaaaccaa
    21301 ttcgttatcc ttaaaggttg aagtaaacgg gattgtctac catttgtcag aatcggatca
    21361 tcgtattgaa gtcgatgtga ttcaagactg tttgcctaga tatattcatc attattcgac
    21421 gtttaaggtc attgtagtat cctttgctcc catactggct aataagcgtt taagtatgct
    21481 tgttcaacag gtttacattg tgaatgaaac acacgaaagc cttcaagttt cagtgaaaga
    21541 tgtgccttcg tatcaagaga agtattcgga tcagcaaaat gtgttgattt ctcaaaaagg
    21601 aaataaggac acgatcgcac aacaggaagt ggcaagcttc gccattgctt ttgtggaccc
    21661 aaatgctttt gaagaagtaa tgacttttca ggaatctgat atcgaaaaat ggctgaagga
    21721 aacattgaac tattttgctc agctatttgg gcagctgtca atgccaaacg atagagttgt
    21781 ccatctctat cgtcgtgcac tttatcaaag cttttctagt ttcggtatga atcgacaggg
    21841 acaggtcgtc ggcagcaatt ggggcagcta tcctgctact aatcgaattt ggaataagga
    21901 tatgtattac gcatcattgc cattcaccat gtttgaaccg gagttatgtc aaaaaacggt
    21961 tctatggttt gatcaatatg gcgttaagtt tcctggttca aaatttacag gaggtatcaa
    22021 tcattctctg agtaattcgt tatccagtgc gttactggct agtctttatt ttgaacatac
    22081 tggggataca aaattctttg agcggtgtcc gcaggttcta ctgaatgctt caagaattat
    22141 tgaggacata ttagctcaac gtcggtcagg ggaaccaatg ctttttccat ctgtttggtt
    22201 atcagacgcc tttgcacttg gaaagtatca taccggcagc aatctttgtc tttggaaggc
    22261 ctgtattgga cttgcagaaa tttttgaagc cctagaacaa ctgagtctta gcaagcgata
    22321 caaaaacatt gcctgtaaac taaaggaatc aatttgtaaa caaatgacta tcgatggtcc
    22381 ttttggtgag caatttttgg agggaatcgg tgatgaggag aaaacaacct attcagtgaa
    22441 gcactatcaa aaaccgattc ttgagcaagg gttaatcttt ttaagtgatg tgattgtgga
    22501 tggaaaaatt aatttattga tgcatgatgg agaagaatca gacactacgt tgatgccttt
    22561 ttatcaattt ttagataata aagatcattt atatcagaac accatggcct tttctgctag
    22621 ttctttcaat ccaacctata gcgaggaaat caaaggcatc acttggggat tagaatccgg
    22681 cgccactttt cctggcttta tcactgtttt aatgagcgag ctgcacaacc ctcaagcttt
    22741 cgtaacccga ttagaagaac taatttgttt ggcagattta gacggttcgt ggtggtggtg
    22801 gccgtatcgc ttagaggctc aacaaggaga agtcgtgcga gactttggct gcggcaaatg
    22861 cggctgggca tcgggtcttt ttgtcagcct aatgatcagt caatatttcg gattgaaatt
    22921 caaaaaagga gtattgcaaa ttgatcctgt tgataattta acgttttctt ggaagaattt
    22981 gaagttcggt caagccttca tttcgattga atgtacgcaa tccgagttga gtatttcaaa
    23041 tttgtcagaa aagccattga aaataagtga tacgaaaaaa attcttcacg tagaaaaggg
    23101 aaaaacgatt cacattcaaa ggagaaaaag atgagtatac taaaaaatat tacccaattt
    23161 aaaaaaatcc agagtcgagc aatcactccc gaaaatccaa caggcgagaa aggtaaagct
    23221 gcgatgttgc caagcgagct aggaccaagc cgcaaaggtc gtggcgcaat tccgctgact
    23281 tctggtgaag aaactgtgat tgcggatatt actggaacgg gaataattcg tcatatgtgg
    23341 atgacgattc gtgatcgaac agaatttggc agttttgttt tacgtgattt aatcttaaga
    23401 atttattggg atgggtcgga tagccctgct gttgaaagtc ctttagggga tttcttttgt
    23461 aatgggtttg gagagcgttg cgaggtgaac tcgttgccga tagtggttaa tcctacaggg
    23521 ggctttaatt cctatttcga aatgcctttc caaacatccg ctaaaattac gatcgagaat
    23581 ttacatccga aggatatgtc ctcatttttt tatacgataa attacgcttt ggtggacgaa
    23641 ttggaagaaa atagcctata ttttcacgca aattggcatc gggaacgcat taccaaatta
    23701 caggaggatt ataagttaat tgatcgatta aagggtcaag gctattacgt gggtacttac
    23761 cttgcgctta cctgcctaga acgttattgg tggggcgaag gcgagtttaa attctacatt
    23821 gatggggata gtgagtatcc gaccgtaacc tcaacaggtt cagaagatta ttttggcggg
    23881 gcgtgggcct ttcatgagta cgatgagttc ggacgtccac atgcgaaaaa ctattcaaca
    23941 gcctttttag ggtatcctta ccaatcaaat agagatgcca caagagatcg ttttgaaacg
    24001 ggaaaattga atgccgttca tgcctttggt gacgacgcaa tgccacgaca tggtctttat
    24061 cgttggcatc ttcaagaccc catctgtttt accgaagatt tatcggtcac tttacaacaa
    24121 atcggcaacg atgatgttcg cttatttgag cggcaagacg atgttgctag tgtcgcttac
    24181 tggtattcaa cagaagcttc aggaaatact aaacgtcatt tcactgatcg agaacgcttg
    24241 ccgcgttaat gaagtgtttt tcacatattc gaagggagga acctatgatg atatatgggg
    24301 caattgaagc tggagggact aaatttgtct gtgcagtcgg tactgaggaa ttaacgattt
    24361 tggaaagagt cagttttccc acaacaactc cagttgaaac aatgaagcag gtcattgatt
    24421 tttttaaacc ttttaaggaa caattggcag gcatcggggt cggctgcttt ggtccaattg
    24481 atattcaatg caaatcaacg agtttcggtc atatcacatc aacaccaaaa cttgcttggc
    24541 aaaatttcaa ttttgtagga acgctaaagc aatatttcaa tattccgatt gaatggacaa
    24601 cagatgtcaa tgcggcttgc tatggtgaat atgttgctgg ttctggcaaa gggttagcag
    24661 gtgttgcata cttcactgtg ggaacaggta tcggaggtgg tgccttgttc aatgggactt
    24721 tcgtggaagg gttcagtcat ccggaaatgg ggcatatgtt gatcaagcga catccaaagg
    24781 acaatttttc aggaaactgt cccttccacc aagattgttt agaggggatg gcagcaggac
    24841 cagcgattga aaaacggttg ggtgtaaaag gccagaattt attggctgac gattcgtttt
    24901 ggcagattga agccttttac cttgcccaat gtgcatacaa tacaacactg atgttttcgc
    24961 cagatagaat tatttttggc ggtggtgtaa tgaaacaaga acacatgaag aaaaaagttc
    25021 aggataaatt tgttgagctt ataaatggat atgttgaaat accaccaatt gatagctaca
    25081 ttattacgcc cgaattaggt gataatgccg gaataatcgg cggattagca ttggccagaa
    25141 aagcggtaag aaataagcaa ccataattaa tgaatgtttc attgatactc atgtcaaaag
    25201 tatttaaccg agtaatcaaa tgatcaaatg aagttggata aagcgaaaat gaattcaaat
    25261 aaaaaggctc taagtcaaat cgtgttgttt tcattaaatc actgaaagga aatgcttctg
    25321 cgtttccttt tttctttttg ttgtgcgctg tataccttga ggatattcac tggtggcgta
    25381 cacttaaata gtaatatatt gtgttacttt atgtctgtgg taatagttat agttgcttac
    25441 caatgtatgt tcatttttcg aaatgttgac ttcataatcc ttggcttaat gtttgaattt
    25501 ttttggtgat agattttttc agccgtataa aataaatcgt aattctatta tagagagttg
    25561 tgattataaa aagatagcaa gaaaaaaaaa gaaagtttct atacatttta aatattttat
    25621 tcctatttct attgctaaaa aagtagaaat tctatattta cctttaactc tcgcttctct
    25681 tgcaaatgta gttagcattt taatcgataa taaactcagg tagttatatt accttgaaat
    25741 gattgataat tttgtcactt ctattttttt tgcaataaat tttttatttt agtgaaaa
//
LOCUS       DAFCLG010000026        25613 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFCLG010000026 DAFCLG010000000
VERSION     DAFCLG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.026"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..1343
                     /locus_tag="LND56_001053"
     CDS             174..1343
                     /locus_tag="LND56_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6742993.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1816..2370
                     /locus_tag="LND56_001054"
     CDS             1816..2370
                     /locus_tag="LND56_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6742994.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2543..3016)
                     /locus_tag="LND56_001055"
     CDS             complement(2543..3016)
                     /locus_tag="LND56_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6742995.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3028..3564
                     /locus_tag="LND56_001056"
     CDS             3028..3564
                     /locus_tag="LND56_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6742996.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            3604..3975
                     /locus_tag="LND56_001057"
     CDS             3604..3975
                     /locus_tag="LND56_001057"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6742997.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            4259..5530
                     /locus_tag="LND56_001058"
     CDS             4259..5530
                     /locus_tag="LND56_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6742998.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            5775..6800
                     /locus_tag="LND56_001059"
     CDS             5775..6800
                     /locus_tag="LND56_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6742999.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7094..8203
                     /locus_tag="LND56_001060"
     CDS             7094..8203
                     /locus_tag="LND56_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6743000.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            8286..9182
                     /locus_tag="LND56_001061"
                     /pseudo
     CDS             8286..9182
                     /locus_tag="LND56_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            9198..10022
                     /locus_tag="LND56_001062"
     CDS             9198..10022
                     /locus_tag="LND56_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6743001.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10054..11364
                     /locus_tag="LND56_001063"
     CDS             10054..11364
                     /locus_tag="LND56_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBK6743002.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11512..12771
                     /locus_tag="LND56_001064"
     CDS             11512..12771
                     /locus_tag="LND56_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6743003.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12781..13653
                     /locus_tag="LND56_001065"
     CDS             12781..13653
                     /locus_tag="LND56_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6743004.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13665..14495
                     /locus_tag="LND56_001066"
     CDS             13665..14495
                     /locus_tag="LND56_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6743005.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14535..16718
                     /locus_tag="LND56_001067"
     CDS             14535..16718
                     /locus_tag="LND56_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6743006.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDKTVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16779..17999
                     /locus_tag="LND56_001068"
     CDS             16779..17999
                     /locus_tag="LND56_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBK6743007.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            17996..19456
                     /gene="gtfA"
                     /locus_tag="LND56_001069"
     CDS             17996..19456
                     /gene="gtfA"
                     /locus_tag="LND56_001069"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBK6743008.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19533..20639
                     /locus_tag="LND56_001070"
                     /pseudo
     CDS             19533..20639
                     /locus_tag="LND56_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            20961..21170
                     /locus_tag="LND56_001071"
     CDS             20961..21170
                     /locus_tag="LND56_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6743009.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21163..21312
                     /locus_tag="LND56_001072"
     CDS             21163..21312
                     /locus_tag="LND56_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBK6743010.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21466..22131
                     /locus_tag="LND56_001073"
     CDS             21466..22131
                     /locus_tag="LND56_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6743011.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22415..23500)
                     /gene="xerS"
                     /locus_tag="LND56_001074"
     CDS             complement(22415..23500)
                     /gene="xerS"
                     /locus_tag="LND56_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBK6743012.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23834..24067
                     /locus_tag="LND56_001075"
     CDS             23834..24067
                     /locus_tag="LND56_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBK6743013.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24064..24471
                     /locus_tag="LND56_001076"
     CDS             24064..24471
                     /locus_tag="LND56_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6743014.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24922..25431)
                     /locus_tag="LND56_001077"
     CDS             complement(24922..25431)
                     /locus_tag="LND56_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743015.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT         8766 a         3843 c         4809 g         8195 t
ORIGIN      
        1 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
       61 aaaatgatag cgtaaaatat tttgtgtaaa tagatattta gggtagaaaa aaagaaagtc
      121 cattctgtaa aattaaagtt accacaaccg aaattttata ggaggacttt ctcatgccca
      181 attttactac agaaattatg gaaacactaa tcaataaagg tgatttagat gaattatttc
      241 gatgtcattt agaattagct gttaattcat tattgcaggc tgaattaaca gcttttcttg
      301 attatgaaaa atatgatcgt gctgggttca attcaggtaa ttcccgtaat gggaattatt
      361 cacgttcatt cagaacagag tatggagaat taaatttggt aattcctaga gatagaaatg
      421 gtaagttttc tcaacaaact ttacctgctt ataaaagaac caatgactct ttagaaacca
      481 ctattattca gctatttcaa aaaggaatta ctatgtctga aatctctgaa ttgattgaaa
      541 aaatgtacgg gcatcactat acgccacaaa ccatttcaaa tatgactaaa atagtttctg
      601 aagatattat tgcctttaaa gaaaggtcct tagaatccag atactctgtc atttttatgg
      661 atgctactca cattccttta aaaagacaaa ctgtttcaaa agaagcagtt tatattgtaa
      721 ttggcattcg attagatgga accaaagagg ttcttgggtt tagtcttgct ccaactgaat
      781 ctgcttatgt ttggaaagag attcttcaag acctaaaaga ccgtggttta aaagaggttt
      841 tattagtcgt aactgatggt ttaagtggca ttaacgatag tattcatagt gtttatccaa
      901 atgctcaatt tcaacaatgt tgtgtccata tatctagaaa tatcgctcat aaggttcgtg
      961 ttagtgatcg acaagaaatc tgtagcgatt tcaaattagt ttatcaagca agttcaaaag
     1021 aagaagcaaa taatcaaata aggtttatga tagataaatg gaaaaagcag tatccacgag
     1081 tggttaaatt gctcatgaat cctgctatat taacctttta taatttccct ccgtcaatca
     1141 gaagaactat ttattcaact aatttgattg aaggttttaa caaacaatta aagaaatata
     1201 ccaaaagaaa agagcaattt cctaatgaag aatctctgga gagatttcta gtatctcaat
     1261 tcaacaacta taatcaaaag tttctatgcc gagttcataa aggttttaaa gaaatacatg
     1321 atacattaga atcaatgttt taacttgatc aacagaatgg gcttttccat ttacacaaaa
     1381 ttattgacgc tcccgacaaa attatgatct tgcgtaaagt tataaaatat taataaaaat
     1441 attttattaa tgaaaagctt ggtagcacgg ttattcatat gctaatttat ttcactttat
     1501 agtaagggga gttcttagta tattgttcaa acttaaattt catataattt aattatgtaa
     1561 attagctatt ctaagaactt ctacggataa ctgcccgtct aaaatttaaa taagagctta
     1621 gaatcttcat ataagtaagg ttaatttttt tgtacaaaaa agtcggaata ttctgagtta
     1681 ctgaaatgat ataaaatcat aaactaaatc ccttgatttt attgactttc aaaagtttat
     1741 atatataagt taatgattat ggtaaaatat atacataatc attaactcag aatattttta
     1801 agaagggaaa caatagtgag ctataatgta caaccgttaa ggacaaatga agagatacaa
     1861 gattttcagt tttggctaag aagaacaagt aatccggaaa gagatagttt tttatttttg
     1921 tttggaatca ataatgggtt acgtatgtca gatattatag gattaaaagt tggagatata
     1981 cgaggcaaaa gtaagcctat tattgttgaa cataaaacag gtaagcgaaa accgattttt
     2041 atagataatt tgagagaaga aattttatta tatactgaag gtaaagaaga aaatgattgg
     2101 ttatttccga gtcggcaaca aggtcgacat ataacaaggg atcgtgtcta tcagatatat
     2161 gcagatatag cagaaaaatt aggacgagat gatataggaa cacatacatt aagaaaaact
     2221 tttgggtatc actactacaa gaaaactaga gacattgcta ccttaatgtt tatttttaat
     2281 catagctcgc aagcaattac aaaaaggtac attggtataa ctgaagatga aataggagca
     2341 tcacttcgtg gatttaaact aggtgtataa tttaaaaatt tttataaaca gtaaagacaa
     2401 agattaaaag ctatttttat ctagaacgat tttatttcat aaagttcgcg gaataaaagc
     2461 tggggttact ggtacttggt ataggttcgt gttatataaa ctaagtaatt aaataacatt
     2521 tttaggttgg aaaaggatga aattaaaaaa agagaatcaa tacggtctca aaaacgcaaa
     2581 cgctgcggaa taagaaaaat actgataact caacgtttag cagactatct aatagggata
     2641 gtctgctttt ttcgtttttg accatacttt tgaccttctg caaacttcct tgcttttcct
     2701 ctgtagaact catgaaatct gcaaaacgaa ttgccgtttc ctctgtctgc tttttcgcga
     2761 tatgggtgta tatatcatcc atagtggttt taatatctgt atgtccaagt cgaacataca
     2821 cttctttgac agacgcacca gattcaaaga gcagactaca atgggtatga cgaaaaccat
     2881 gaagtgtgat atgttttaag ttattctctt taatgatgat tttcaaattg tgatttacat
     2941 agtctaaata aaggggctta ttttcttctg atgaaaacaa aagttgttca ccatctttta
     3001 taggtttgat tctcattttt agatattatg cccgcacgta ttcatcacat ttctattgtg
     3061 aatcgcttta ttcgaccgac atttgacttt tatcataata tactcggttt aaaattacta
     3121 atgaaaacaa ttaatcaaga cgatcatacc atgtatcacc tgtttttttc agataatcac
     3181 caccgtgtgg gaactgaact gacttttttt gaagtacaag aagggaacaa ccagtttttt
     3241 ggaaccaata cgattgaacg aacgatacta aaagttcctt ccgaagcatc attgcatttt
     3301 tgggaaatat attttgaaac acaaggcgtt tgccattatg gaatcgaatc attttctgga
     3361 cgacctatct tacgttttga agggccagat gcaacacgat tagctttagt tcccttacgc
     3421 gaatttgaag atattgatga ttatttcccg tatgtcacag accagatccc tacagagcat
     3481 gccattttag gaattgatgc cattcaacta cgcgtacaat attccgatgc gactgaacga
     3541 gagttattgt cggtaggctg gtagcacaaa gaatacgtca gtttcttttc gggtcatcaa
     3601 cgagtgacaa tcttagaaaa tcaagacact cagttctatc aagaagttca gattattcaa
     3661 gatcgtgaga atccagttgc cattgaaggg attggtagcg tccatcatgt tgcgtttggc
     3721 gtagaaaata aatctgattt acaacgcatc gacaaacaat tacaagagcg caattttata
     3781 aattcaggaa taaaagatcg tgaatttttt gtatcacttt attatcgtga cccaaatcaa
     3841 ttattgattg aaattgctac aggggaagga aacctagatg caaaagcgta tgaaaatcaa
     3901 agtccacgct ttgaagaaat tcctttattt ttaccgcagt acttaaattt cattcgtgaa
     3961 caagtggagc aataattagc taatcaaatc catgacgaag agtaagaggt tgactcataa
     4021 gtcaaaaaat aaagaaaaac gcatcaaatc cattctaaag aatgttgatt tgatgcggtt
     4081 cctattttta taaactatca ttttcgacta ataaaactac ttatgagtca gcctctcttt
     4141 ttttcttgat ttttatacct ctttctaggt aaactaaaca tctgccaaaa ataccgtgtc
     4201 tcattgaaac attctcaact atttcaaaat aaaggggagt accaaatgaa cgagtatatt
     4261 gaaattatcc acgcaagaga aacaacttaa aaatgtcagt ctcaaaattc ccaaaaataa
     4321 aattagtatt tttaccagcg tatctggttc aggaaaatca tcgattgttt ttgatactat
     4381 tacccaagaa gctggtcgtc aactgaatga cacctatgat agttttaccc gtttattttt
     4441 acctaaatac agtcgtccag aggttgatga gattgataat ctttctacgg cgattgtggt
     4501 caatcaaaag aagattggtg gcagtgcgcg ctcaacgtta gggacgatta ctaacatcaa
     4561 tgcgttattt cgtgtgttgt tttcatgttt tgcaacacct agtttaggat atgcaaatgc
     4621 ttattcattt aatgatccta gtggaatgtg ttctgtctgt cagggaatcg gtaagacgat
     4681 tgcattttta ataggattgc tttatttttc ggctattgat aatgctgatt ttgtattttc
     4741 tgatccattt accgcagaag ttttgtctgg aattgaggaa caggcaaaga atcatggtta
     4801 ttttgtattg gtgcataatg ttaattcagc agaagatgtt aaaactattc agagaaattg
     4861 gcgttttgag gggttcattg ttgttggtgt acgtgtaaaa gaatttttgg aattaaataa
     4921 cgtttttgat ggtcctgttg tttatattga tacgcatctt tcaaaagaga cattaagaga
     4981 acttgaacaa caaatgaatc gtttatttgt aaatacaaat gattaccaag ctagtacatt
     5041 ggcagtggaa catttgattc attatggtca tgagaaaata gcgtttcttt cttttgacta
     5101 cattgtaggt gaaccaagtg ttattcaaga acgacatcaa gcttatatcg atacattaaa
     5161 gaaatatcat ttaaccttta aatcgcattg gatatataac aactctgaat ttgagcgaat
     5221 ttatcaagaa ttagctgaat ttactgcaat tgttgttact ggagattatt tagcggctaa
     5281 attagtaaaa tttttaaaaa ataagaataa acgagtgatg gaacaattat cgattattgg
     5341 atttgatgat attacttttg cagaattaat ggatcctgcg ttgacaacaa tacgactaaa
     5401 tccaggtaac aagggggcga ttgcttttca ccaactgatt ggactggcca aacaagaaga
     5461 aataaaaaat caagtactac tattagaggg agaattgatt tctcgagact ctgttcacaa
     5521 aatcaaataa aaaagctctg tgaaataagt atagttaaca ataaaatatc cgaacgatag
     5581 agagaatgaa ctgtgatcta gcagttcctc gtcgcaaatt cactagaatt agcattattt
     5641 actctaaatt gttcggatat tttttcttgt ttttaattat gattacagtg cctattttta
     5701 acgaaatttg cttattttca aaatgtatga ttatatgtta gtaaacactt tcaaatatga
     5761 aaagaggagc tattatggta ggaattaggg atgttgcaaa acgggcgggt gtgtccattg
     5821 caacagtatc gcgcgtgcta aatgaggatc cgacactttc agtaaccgat gaaaccaaac
     5881 gaaagattgc agaatcggtt aatttttatc attatgtcaa aaaaagtccc gttgcaaaaa
     5941 atttaacaaa tattgcatta ataacgacag tttctgaagt agatgagttg gaagatccct
     6001 attttcgaac gattcgcaga gggattcaag tcgaagcaga acaaagcaaa gtcaatttga
     6061 agaaaattat tcgtttgtca gaaacaccgt tggagcaaga cgatttgcta aaatgtcaag
     6121 gtgtgttgat tattgggcag gtgctatctt ctgtgattga agaagtatca gcaattaatt
     6181 cgaatgtcgt agtgattgat gaccctagta ttgaagctga agtagatgca gtttatactg
     6241 atttaacaaa agcaacgaat cagcatttag atcgattgta taaaaaagga caccgaaaca
     6301 ttgcgtttat cggaggagga cgtgttcgtt tagatagcca aggaaataaa cacgtgtcag
     6361 atgatgatta tcgaaaaata gcctatgaga cgtggatgaa acaaaaagga ttagaaaaat
     6421 atagtcaaat ttatttagaa ggctggacca cacttgacgg aatgcaggca gcagatttac
     6481 ttttgaaaaa acatgctaaa gataagttgc cgactgctat catggtagga aatgatccta
     6541 ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct acatattcca gaagatattt
     6601 cgattgtgag ttttgataat attgaagttg ctgaattttt aacacctgca ttaaatacgg
     6661 tgaatgtgaa tacagaagaa attggaaaac tagcagttcg catggttaat gaacgaataa
     6721 aagaagttag aaatgtttcg atcaaagtga ttgtttctaa taatattatt gttagagaaa
     6781 gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa gggtgtttac ttttttgttt
     6841 actaatattt ttagtaaaaa atctcgacaa aacattaagt aaacagttat ggtgttgtta
     6901 acaaaaagaa aacgcattca aaacaggagg aattaagatg aaacatttca aaaaaatagc
     6961 tttaacaggg atagctgttt taactgggac attactcgca gcatgtggtg ggggagatag
     7021 taattctaaa gacgggcaag taactttaag ctatgcgagt tgagatactg accaagctcc
     7081 aggactaaga aaaatgttag atgaatttga aaaacaaaat cctgatatca aagtagaaat
     7141 ggagacaacg ccttgggatc aatattgggt taaattagaa gctgcaacaa cgggaggtaa
     7201 tatgccagat gtagtaacca tgcactctag cgaatcctat aaatatatgt cacagaatgc
     7261 tttgatggga cttgatgatg ttatcaaaga aaatgatatt gacatgggaa actttactga
     7321 aggaattgct gatttctata cgcttgaaga cgaactatac gcaatcccta aagatgcttc
     7381 tgtagtgggg ctatggtaca acaaagaatt atttgataat gcaggtgtcg attatccaga
     7441 tgaaacatgg acatgggaaa cactcagaca agctgcaatt gatttaacag atgaatcaaa
     7501 aggcatctat ggttttgcat cacatattgg aacagagact ggctactggc catttattta
     7561 tcaaaatggg ggagaagtat ttgccgatga caatacaaaa tcaggtttga ataccccaga
     7621 agcacaagaa gctttagctt tttatcgaga tattgcactt gttgatcatg cttcaccaac
     7681 aattacagaa ttacaagatc ctgataagtt aagtcgtttt caatcaggta gattagcaat
     7741 gatgattgaa ggaaactggt ttgcaccggg atttttgaaa aatgactatc tagttgaaaa
     7801 tgttgcttct gccgtgttac ctaagggaaa agttaatgca acaattacaa atggacttgg
     7861 ttgggctgcc agtgcgagta caaaataccc agaagaagtg aaaaaactaa tggcattttt
     7921 agctagtgag gacgccaata aaatccaagc ggaaacgggg gcatcaatcc cagcactaaa
     7981 aggctttgaa gacgagtggg ctgcttcttc agatacaatt gatttgagtg cttttggaaa
     8041 aatgttggag tatggagtcc ctcgtccata caataaagat ggattacgag ctgaacaagc
     8101 agaagttcaa attattaacc gtattctgtc tggtgaagta actgttgaag atggaaccgc
     8161 tcaaattgaa aaagaagtca atgcgatatt gagcggaaat taaattagtt aattatacaa
     8221 tatacaggcg aaaggattct atttcgtctg tatattaatg gatgaatacc aaaaaggggg
     8281 atattatgaa aaatcgaatt tttggaaaat atcgtaaaga tcaagtgata tggggctata
     8341 tttttatttt tccaacggca ttaggattaa ttttattaaa tatatggcca gcaattcaaa
     8401 cagcaatttt aagttttcaa aaaacggtag ggttcggtga tactgaatgg gtcggattaa
     8461 gaaattacca aaaaatgttt gcagacggtg aagtttttca gtctttatta aacacattaa
     8521 tttatgcagt ggtttcgata cctagtattg ttgtattatc cttgttggtg gcagttttaa
     8581 tgaatagaaa agttaaagga ttgtcgattt atcggacaat ttattttcta ccagtcgttg
     8641 ctgcaccatc cgcagtcgca atgatttggc gctggatgtt caataatgat tatggactga
     8701 ttaataacat gttggctaaa ataggtctcg atggaccagc atggttaacg gatccatcga
     8761 ttgcgattta ttctattatc attgtaggag tttggagtgc tattggttat aacatggtgt
     8821 tattactagc cggcttataa gaaattccta aagattatta tgaagcagcg gaaattgatg
     8881 gtgctagtcc aatccagcag tttttcaaca ttacgttacc attagttaca ccaacattat
     8941 tttttgtagt tgtaacaaca gttatcaatg ctttccaagt atttgatgtt atttttatga
     9001 tgattactcc taatagtacc gccatgttta aatcacaatc attagcctat ttattctata
     9061 aacattcatt tattttaaat gacaagggat atggttcggc aatcgttatg ttccttttag
     9121 tgattatatt aattattact gcaattcaaa tgaagttgca gaaaaagtgg gtaaactatt
     9181 aaggaggcta tcaaattatg aaaaataaat ttttttcaaa tgcgccaatc catatcatgc
     9241 ttattttagg tggtattgca atggcattac cttttatttg gatgatttta acttcaggga
     9301 aaacgttggt tgaatctacc caaattccac ctacaatttt ccctgagaaa tttcagctaa
     9361 gtaactatca acaagtactt gatttattac cattcatggc tttttatatc aatacatttt
     9421 taatggttgt tggacgagtg attggctcgg ttttattttc agcaatggct gcatatgctt
     9481 gtgcgcgtct aagttttcca gggaaaaata tccttttcag cttagtactt attcaaatga
     9541 tgattcccgg ttccattttt attactccac agtatttatt agcacaaaaa ttgggtattt
     9601 taaatacaat tccagcatta atattaccag gggtagttag tgcgtttggt acgtttttat
     9661 taagacaatt tttcttaggt ttacctgatg aattggagga agcggcctat ttagaaggtg
     9721 ccaatatttg gacgatcttt ttcaaaatta tgttgccgct tgctaaatca ggattggttg
     9781 cacttggaat attcacagcg ttgtttgcat ttaaagattt aatgtggcca ttgattgtca
     9841 atacttcaat tgataagatg cctttagctc cgggattagc aaacttacaa ggacaatatg
     9901 ccacgaactt cccacaatta atggcaggtt cgttattagc aatttggcca atagttgtga
     9961 tattcatcgt tttccaaaga aaattcattg aaggaattgc tacaacaggt agtaaattat
    10021 aaaactatta attagtgatt ggagagtaag tacatgacaa aaattacatt tattggtgca
    10081 ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga tgttaactcc aagtctacaa
    10141 gatgcacata ttgcattgta cgatattgat gaaaaacgac taaaagaatc aaatttaatg
    10201 cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg atattgtttc ttattatgat
    10261 cgcaaagaag cgttaagaga ggctgatttt gttatcaatg ctattcaagt gggtggttat
    10321 aaaccttcaa cagtgattga ttttgatatt cctaaaaaat atgggttgcg acaaactatt
    10381 ggtgacacca tggggattgg tgggattttt agaaccctac ggacactacc agtaatgtta
    10441 gactttgcta aagaaatgga agaagtttgt ccaaatgctt ggttattgaa ctatacaaat
    10501 ccgatggcga tgttaaccat gggtgtttta aaagcgacga gtattaagac tgttggttta
    10561 tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc atttaggaat agccgatcaa
    10621 tatgacttaa atgactttca atggaaaatt gcaggcatta atcatatggc attcttgttg
    10681 gaaattacaa aaaatgggga agatttctat ccaacaattc gtcagttagc tgcagaaaaa
    10741 ccaaatccac atccagattc tgtacgtttt gaattgttga aacgttttgg ttactatatt
    10801 acagaatcaa gtgaacacaa tgcagaatac catccatact ttattaaatc aaaataccca
    10861 gaattgattg acgaattagg gattccgttg gatgaatact tacgtcgttg tgaacaacaa
    10921 attaatgatt gggaaagcat gcgagaggac attgtaaata atggggagtt aacacatacg
    10981 agaagccgtg aatatgcttc atatatcatg gattctatta cgacaggaac tccgtcagtg
    11041 attgcaggaa atgtcttaaa taaaggctta atcacaaact taccgacaga tgtttgtgtg
    11101 gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa cctatgtcgg tgaattgcca
    11161 acacaattag ctgctttaaa tcggacgaat atcaatgttc aagaactgac agtagaagcc
    11221 gctttaacac tagataaatc aaaaatttat caagcagcgt atttagatcc acatctatca
    11281 tctgaattgt ctttaacaga tatcaaaaat ttagtggatg atttaatcga agcacatggg
    11341 gactatttac ctgaatataa ttaaaaagaa gctgaaagag taaggaattt tcaattcctt
    11401 actcttttct tagttaaatt tagtaaaaat attgacaaaa cacttagtaa acagttacat
    11461 tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg aggagcataa aatgaagtgg
    11521 tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa cgatctcttt gactgcttgt
    11581 ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag agttttttaa tcaaaaaaaa
    11641 gaaatgactc aaacgattca ggagattgct aaagattttg aagcaaaaaa ccctgatatt
    11701 catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa ttaaaacacg tatgcttgca
    11761 ggcgatgtac cagatgtcat taatctttat cctcaaagta tagaattgaa agaatgggcg
    11821 aaagctggtt atttagagga cttaaccgaa gaaccgtatt tagagaatat caaaaatgga
    11881 tatgcacaac gttttgctat tgaggatcgt gtatatagca taccattaac cgctaatgtc
    11941 tatggttttt actataacaa aactgctttt gaggaaatgg ggattcaagc accagaaact
    12001 tgggcagaat ttgaaaaaat agtagctgag attaaagatc aaaacaaagt accgtttgcg
    12061 attgcaggag cagaaggctg gactttaaat ggttatcacc aactagctat agcgactgct
    12121 gctggtggtg aagaggaagc gaacaatgtt tggcgtttct cagaagttaa tggaattaat
    12181 gctgaaagca aagaaatgca aaaagatttt aatcgattag atttgttaag agaaccaaaa
    12241 gctttgcaaa acaactggca aggtgccggt tataacgata cagtagttac ttttacaaaa
    12301 ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc cgatgattca ttcacaaaat
    12361 ccagattttg aagtaggaac ttttcctttt ccagcagatg aagcaggtca aagtctaacg
    12421 attggcgcag gagacctagc cttatcagtt tctgcaactt ctcaacataa agaagaagcc
    12481 aaacgttttg tagaatatat gacaacacct gaagcgatgc agaaatatta tgatgtagat
    12541 ggtgcaccgt gtgctgttga gggcgttatc gagaatactc aggattcacc attaagtggt
    12601 ttaactgaac ttgcttttac tgatcgtcat ttagtttggt tagccaaaga ttggaatagt
    12661 gaaaatgatt tttatacctt aacaacaaat tatttacaca atggcaatca aaaagccatg
    12721 attaacgcat taaatgcttt ctttaatcca atgaaagctg acggaaggta ggaaaataaa
    12781 atgaaaataa aagaaatttt aaatcgttac tgggcaatca tttttgtagc aattccaatt
    12841 attttgcaga tgattttttt ctactttcca atggttcaag gtgcttttta cagtttaact
    12901 aattggactg gtctgactta taattttgat tttgtgggtt tgaacaatta taagattctt
    12961 ttttctgatc cgaagtttat caaatcgatt ggcttcactt taattgtgac gctttgcatg
    13021 attgctggac aaattggttt tggtattttg attgcgcggg cgttgaacgc aaatattaaa
    13081 gggaaaacat tctttagagc atggttcttt tttccagcgg tcttatcggg cgtggttgtt
    13141 tctttagtat ttaaacaaat ttttaactat ggtttaccaa aaattggtag tatgttaggc
    13201 attgaatttt tacaaaccag tcttttagga acaacatggg gagcgatttt tgcagtaatt
    13261 tttgttttac tttggcaagg aattgccatg ccagtcatca ttttcttagc gggttttcaa
    13321 agtattccac aagaaattat tgaagctgct agaatggatg gggcgaatga taagcaaatt
    13381 ttttggaata ttgaaatcac ctacttatta ccaagtattt caatggtatt tattatggca
    13441 ttaaaaggtg gcttaacagc atttgatcaa atttttgctt taactggtgg tggaccaagc
    13501 gatgccacaa catctttggg gttacttgtt tataattatg cttttaaaaa caatcagttt
    13561 ggttatgcga atgctattgc tttagtctta tttatcttaa ttgggattgt ttcactggca
    13621 caattaaaat tatcgaaaaa atttgaaatc taggaggttt cccaatgaaa acatcaaaag
    13681 aaaaaaatat ttctaaatac ttgcttttat tattaggggg cttcatgatt ttagttccct
    13741 tagtttcaac gctattcggt tcatttaaaa caacacccga tattatgaat aatttctttc
    13801 gttttcctaa tccagcgact ttggaaaatt tccgaagatt gttagcggat ggaattggtc
    13861 attacttttg gaattcggca atcatcacaa tcattgcagt agttgctgtg acagcaatta
    13921 ttcctatggc tgcttattca ctagcaagaa aaatgactcg tgggaaagca tacggattga
    13981 tgtatttatt tttaatttta ggaatttttg ttcctttcca agtaattatg attccgatta
    14041 ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg attaatctta ctctatctca
    14101 cttatgccgt accacagaca ctatttttat atgttggttt tattaaacta aatgtgccta
    14161 tcagtctgga tgaggctgct gaaattgatg gggctagtca ctttacaact tatttcaaaa
    14221 ttgttttccc aatgttaaaa ccaatgcatg cgacgactat tattcttaat gctctatggt
    14281 tttggaatga tttcatgcta ccattattaa ttttaaatcg tgattctaat atgtggacac
    14341 taccattgtt tcaatataat tatacgggac aatattttaa cgactatggt ccaagttttg
    14401 cttcctatac agtaggaatt gtcacgatta cactagttta tcttgctttc caaagaagta
    14461 ttatagctgg aatgagcaat ggagcagtga aataaccaat aaattattct cattataaag
    14521 gagttcataa acttatgatt atatttgaca atcacacaca aacatttcat ttacaaaata
    14581 aaaaaattag ctatctatta ggaatcgaag aaaataatta tttaacgcat ctttattttg
    14641 gaaaaaagat tacgcactat tctggtgggt atcgctaccc ccgaacagat cgttccttct
    14701 cacccaacaa tgcaaatgcg caagatcgtt tattatcttt agacacatta ccattagaat
    14761 ttccaggata tggacatggt gattttagag aaccggcgca taatattaaa ttaggaaacg
    14821 gtagtcgtgt aaatgatttt cgttatgata gctacgaaat tcaaaaagga aagcccagct
    14881 tagcgggatt gccagcctta tatacggaag atgatgatga agctgaaacg ctgattattc
    14941 aattgaaaga cgcgattagt ggattgactt tacagttgtt ctacacaatt tatcaaaatc
    15001 acagtgtaat tattcgttct acgcaattag taaataatag tgataaaaca gtggagatta
    15061 accgcttagc tagtcaatca attgattttc caaatcgtcc actcgagtta attcatttaa
    15121 atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa aattcataca gggataaaag
    15181 tattagatag taaacgcgga tcaagcagcc atcaacaaaa tccgtttgtt gctttagtag
    15241 atgccaaaac agatgaattt caaggagagg catatggatt taatttagtt tatagcggta
    15301 atcacgaaac ggtgattcaa aaagatccat ttgaccaaac acgggtcatt atgggaatta
    15361 attcatttaa ttttggttgg caactaaaaa ctggcgactc atttcaaacg ccagaagtcg
    15421 tgatggttta tagtaatgaa ggtctgaata cgttatcaca gacgtatcat gaattgtata
    15481 atcaccgttt aattcgtgga aactatcgtt taaaagaacg tcccacgtta atcaacaact
    15541 gggaagcaac gtattttgat tttaacgaag aaaaaatcat gaatattgtc gatgatgcga
    15601 agaacttggg cattgaaatg tttgttttag atgatggttg gtttggcggt cgtgaaaatg
    15661 acttcacttc attaggtgat tgggttgaaa cagaaggcaa acttgcttgt ggtttggaaa
    15721 atttagccaa gcaagtgcat gataacggga tgaaatttgg gatttggttt gaaccggaaa
    15781 tgatttctga agatagtgaa ctatttcgag cacatccaga ttgggcattg ggtattccag
    15841 gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga tttttcaaga caagatgttc
    15901 gtgataatat ctatcaacaa atgacggcga tttttgatcg tgtaccgatt gattatgtga
    15961 agtgggatat gaatcgaaat atgacagaag tctattctgt cttgttggac tcaacgatgc
    16021 aaggtgaagt ggctcatcgt tatatgttag ggttatatga atttttagaa aagttaacaa
    16081 cgaattatcc aactatttta tttgaaagtt gttcgggtgg aggcggtcgt tttgatgcag
    16141 gtttcttgta ttacatgcca caaacttgga cgagcgacaa taccgatgcc gtggctcgtc
    16201 tgaaaattca atatggaacc agcttagtgt atccaatttc aagtatgggt gcgcatgttt
    16261 cagcagtccc aaatcatcaa acaggaagag aaacgagttt aactattcgt ggcaatgctg
    16321 cgatggctgg tgtttttggt tatgagctag atctaacgca attaacttct gaagaaaaag
    16381 cagaaattaa agaacaagtt gccttttata aaaagcatcg ccaacttttg caatatggaa
    16441 ctttttatcg cttggaaagt ccgtttgaag gcaatgatac tgcatggatg tttgtctcaa
    16501 aagaccaaac agaagccatg gtcttttatt tccgagtatt agcagaagct gcgcatccat
    16561 tagtcacatt gaaattagca ggattagcag aagaagcaac ttatcaaatg gaagatgtaa
    16621 catttgctgg cgatgaattg atgaatctcg gtttttatat tgactcagaa ctacatggtg
    16681 attatgcgac acaacgcttt tatttgaaac agatatagta gcttgaattt acaatataag
    16741 tgcaacacca aaagagactc attccaaacg aagaaaatat gttttattta cacgcattta
    16801 ctaaaaagca gcctgatttg aattgggaaa atgaagaagt acgtgaagaa atctatgcaa
    16861 tgattaacta ttggttagat aaaggactag gcggatttag aatcgatgcg atattaaaca
    16921 tcaaaaaaaa actagagtat ggcatctttg aatctgatgg agaagatggg ttagccttca
    16981 ttgggccctg gattcttaat cagtcgggca ttgaagtgtg gttaaaagaa atgagagagc
    17041 ggacatttaa accacataat agtatgacgg tagcagaagc cgatgttcct aatgaacgtt
    17101 tagatgaata tattggtgaa gatggctatt attcgatggt ttttgatttt agttatacgg
    17161 atattgatgt acctgaaact ggtgaatggt ttaaagatag ccaatggaca tggacggata
    17221 tgcgtaccaa tatttttacg aatcaattag ttacgcaaga taaaggttgg ggcgccttat
    17281 atttagaaaa ccacgatcaa ccacgttcaa ttaataaata tattccagaa gaatggatca
    17341 atgattatag taaaaaaatg ttggcgacgc tttttatgtt actaagaggt acccctttta
    17401 tttatcaagg acaagaacta ggaatgacca acatagaaat ggcatcatta gaagattttg
    17461 atgatgtagc tacgcattct cagtataaac gggcattaga atatggattg gcaccagagc
    17521 aagcattgaa agctgtcaat aaacgaagtc gggataattc tcgcacacct atgcaatgga
    17581 atacgcaaaa aaatgccaga ttctcaactt ctgataatgt atggttaaaa gtaaatccta
    17641 attatccaga actcaatgca gaagctcagt taaccaatca acagtcggta ttcaattatt
    17701 atcatcaatt aattaaatta agaaaaaata gtgtatacaa agacgtgcta atctacggac
    17761 aatttctacc agtgaaagat aaaaatgaac atattttgct ttatgaacgc caattagaag
    17821 ataaaatcgt attagttatc ttaaacgtta cagcaacaga acaagaatat tatgtagatg
    17881 aacagtacca acaagtatta atcaataatt atgaagatgt tttattagat caaagtcgaa
    17941 aattgcgttt acgaccgttt gaaagtattg tattagctaa ttatggagga caattatgaa
    18001 aattaaaaac gaagcaatgc tcatcaccta tgcagatagt ttaggaaaaa acatgggcga
    18061 attaaaacaa gtcttagaca aacatttaca aggtgttatt ggtggtgtac atctgttgcc
    18121 tttttttcca tcgacaggtg atcgaggctt tgcgccgagt gattatacga cagttgatcc
    18181 agctcttgga acatgggaag atgtggaagc attaggtgaa gattattatt tgatgtttga
    18241 ttttatgatt aatcacattt ctcgtgaatc aaaatttttc caagatttca aaaaaaacca
    18301 tgagcagtca ccatacaaag aaatgtttat ccgtattcat gaatttttcc cagaaaatcg
    18361 accaacacaa gaagatattg atttaatcta caagcgtaaa gacaaagcgc cgtttcaaac
    18421 ggtagaattt gcggatggta cgacagaaga ggtttggaat acctttgggg aagaacaaat
    18481 tgatttagat gtgaccaaag aagttgtgaa agaatttatt cgagaaacca tcaaagatat
    18541 ggcgaaccat ggctgttctt taattcgatt agatgcgttt gcttatgcga ttaaaaaatt
    18601 agataccaat gatttcttcg tggaaccaga tatttgggaa cttttagacg aagtgcgtca
    18661 agaagcagcg aaatatgacg tcgaattatt accagaaatc catgagcatt attccattca
    18721 aatgaaaatt gccaatcatg attattatat ctatgacttc gccttgccaa tggtcactct
    18781 gtattcactt tatagtggaa aatcagaacg tttagccaat tggttgaaaa tgagtccgat
    18841 gaagcagttt acaacattgg atacgcatga tggaattggt gttgtggacg ctcgtgattt
    18901 attaacagat gaagaattgg attacacctc tgaggaattg tataaagtcg gggcgaatgt
    18961 gaagaaggtc tattcaagtg ccaattacaa taatttagat atttatcaaa ttaacagcac
    19021 ctattatagt gcattaggcg ataatgaccg tagctatctg ttagcacgtg cgattcaagt
    19081 atttgcgcca gggattccac aaatttatta cgttgggtta ttagcaggaa aaaatgatat
    19141 tgaactgttg gaagcaacga aagaagggcg taatattaat cgtcattact atgatttagc
    19201 cgaaattgaa gaagaagtgc aacggccagt gattcaacaa ttatttgcac ttttaaaatt
    19261 tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt gaagtagcaa cgccatcaga
    19321 atcagaaata gtgattacac gtaaaaataa aaacggtagt gatagtgcaa cattaacggc
    19381 taacttggat acgaaagaat ttatcattga gtctaaagat ataaaaattt tcactagtgt
    19441 tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc tttatactag cacttggatg
    19501 acttatccaa gaccaataaa aaggactcat acatggataa gtataaaaca aatacagcat
    19561 ttaataaatg gttttctgcc ataaaattag gaaaactttc tgtcaactcc caaaaatgta
    19621 tttttgatta caattcgtat cgtaaaaaac tgtcttttga agctgtgctc aagctctttc
    19681 tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt gagtacagct cttatcgacc
    19741 gatcgctcca actagaaacc gatgtgtcat cgattagtca tacccaattg tctcgagcat
    19801 ttaatgcact tgaacctaaa gtgttagaag aaatttttca acaactgtta gaaaaagttc
    19861 aacatcaaat caaaccaacc aagcgaaatc atctgtatct gattgattcc agtacgtttt
    19921 cgcttagttt aaaacgtcat aagtgggcga ctttccgtca aacaaaatca ggtgtcaagt
    19981 tacacttaaa ctattgttat atggacgata ctacgatgta tccaactgat tttaccttaa
    20041 caaatgccca tgaacatgat ctcaatcaac taccagtatt ggtgaatcaa ccagaggcaa
    20101 cttacgtgtt tgatcgtggg tattaggact ttgaaaagat ggatcagatg cattgggatg
    20161 gctatttctt cgtcactcgg attaaaaaaa acaccaaagt tcacgtgata gatactctgg
    20221 aaacatcgcc agaaacagaa attctacgag acgagctggt tcgtttagga tctaaaacct
    20281 atctaaccgc caatttccga ctagtcactg ttcaagataa gaacggacgt gtgtttcaat
    20341 ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc tgatatgtat cacgctcgtt
    20401 ggcaaatcga attgtttttc aagcacatca aacaacacat gaccattaaa accttctttt
    20461 ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac gatgatttcc gctctcctca
    20521 cgtttctcat caaattggag acgaaaacgg aaaaatcagt gttccaaatc aaacgattct
    20581 ttcgttattt actgtttcag ccctttgaat gttgtctaga aaaattaatt cccacatgag
    20641 aaaaaagaaa tttggctcgt tacttttggt tttcagttga ataacagata aaaaattttc
    20701 tggaaaaagt catcctctgc gtcagcacag ttttttttga cttattttca gaggaaatag
    20761 ttttcagaat attctaaaat gttttatgca acactagtga tattttttag aattagattt
    20821 ctaatgtgca ttttttgagt attaaggagc aagtatacaa agatttataa agctgattaa
    20881 atttgaataa aaatatgcag gaacgctact atttttagta gtgttctttt ttatatttga
    20941 ataaaaaatg tggtggttaa atggtggtta taacaaaaaa tgaattaatg aaattaggtt
    21001 atgggaagta tcagtctgag agaatcatac gacaggcaaa agcaattatg gtttcaaaaa
    21061 gctataccta ttacgaaaat agaagattag gactagtacc tgttgaatct gtagaagaag
    21121 ttttaggtat gaagatagag gtagaggagg attttcagaa tcatggttaa ttttaaaaaa
    21181 gataaaaatg ggaaatatca ttttactgct agtttgggat tcgacaaacg aacagggaaa
    21241 agagtacaaa aaaacgtaga ggggttaaaa cagttaaaga agaaaaaaag cgtataccga
    21301 acttgtttct aattatggaa aagaagcaga aacttctcaa agtactatgt tgtttgagga
    21361 gtttttttat aaattattcc taccttatta tcaagggaaa gtagaagaaa gaacatataa
    21421 aaatcggtta tctacaatca aaaaaacttt tcttatttct ttaaaatgga gttaagagaa
    21481 atacgtccaa ttgacattca aaagtggcaa aataatttat tgagcaaata taaaaattct
    21541 tatgtaagaa acatgtatgg gatttttcag atgtcattag atcgagcagt tgttttggga
    21601 ctacttgaaa aaaatcctgc aaaagttatt ggaaatgtga agaaaaggaa aactacagtt
    21661 gatttttgga caaaagaaga atttacaaaa gttatttcaa ctttttatat tgatgatttg
    21721 taccaacatt ttggttttat ttgtatgtgg ttacttttta tgaccgttat gcgtttggga
    21781 gaagccactg cattaacttg gaaagatatt gattttgaaa ataaaaactt gcgtgtgaat
    21841 aaaacactct attataagag agccaaaacc tatgagtttg tagaacccga aacggcttct
    21901 agtattcgta caatcgaact agacgacaat acaattacct ttttaaaagc ttggaaggag
    21961 cgtcaagttt caataggagg catgtcgttt gtactttctt ataatggaac acctactcag
    22021 aaatatacaa tccgtcatat tatgagaaga catgcaacga tcgttggtgt tcatataatc
    22081 cgcatacacg gattaaggca tagtcatgct tctttgctta tatcacaata aaaagatgca
    22141 cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa cgactttagg aacttatggt
    22201 catttgtatc taaattcatt gagagagata acaaatgatc ccacagatta tatccaaact
    22261 aatttttcta aaatcaaacc gacacctatt tacgataata aaaaataaat gataaacaaa
    22321 agtaggatca caccaaaaga aacacactag aaaaactata atacaggctt ttctagcgtg
    22381 ttgtttctta tttccgccta tttatacatt atactcataa agcatttaat gcatcgatcg
    22441 tagccgcatt gtctatatgg gtatataaag ttgttgtatt ggtacttgaa tgtcccaatt
    22501 gttgggcagt taataccaat gaatttgtct gttggtagag tctagtagct aatgtatgac
    22561 gcagtttgtg agggctgact tgaattctaa agccttttga atattttgct accatccgtt
    22621 cgattgcaat accggatata cgcttggctt ctccttggta aattgctaga aataaagctt
    22681 tttcgttttc tgttacttta tatcgttctt tccgaatagc tagataatta gctaaatatt
    22741 ccattgcgaa tgctgctata ttgacagcat ctcttttatt tccttttcta gttactacca
    22801 cattattctt atctaaagat aggtcatcta aattaatatt tataacctcg gagagtctca
    22861 atccagatcc taaaatcagt gcattgatcg ccaggtctct ttctacatct cgttgataat
    22921 agatcaaagc tcgtttactc aaagtatttt tatattcatt ggcaatataa tctagataat
    22981 tttgtgtatc cttatctaga aataattttt tctcaatggc attggcacgt gcagctaacg
    23041 tagttttatc tgtgatggtt gctacttgat ccataggatt attgtctaag tatggttttc
    23101 ctagaggatt attggaactt ttcgaaagat atttaaatag aacctttaat gccgtgatat
    23161 ttctattgat agtagaagta gatagaccta agttattatt atctttgagc ttagtttttg
    23221 ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa atgagatttg tacaagttta
    23281 ggtcataggc agttaactgt cttatcgagg tgatagaagt atcaggaacg atagactcac
    23341 tcagaagcca ttcataaaag agcttataat tttttagata ttctaaagtg gttcgaggtg
    23401 attgatcttt atcctcacga tcttgaacaa aatcttttat ataatcgggc atatgaagta
    23461 tgattgtatc tattttttga tatatttctg tatttttcat gtcattcatc ctttcaataa
    23521 gtaggggaga agagtatctc tataattgga ctataccaat tatagaatgt tttgtcaatt
    23581 ggtatgctct aaaatttatt aatttgtttc aaaaaaagca tataaacagg tatacgttta
    23641 ataatttaaa ataaatggta tacataaata gggaaggaaa tagaagggag caatctaaat
    23701 tttttgaatt ttcttaatta attatataga aagaacaatc tttctataga gttagacatg
    23761 agagggacta tgcttgattt aaaatcaaca agcgtatata ctgtatatac aaagaaaaag
    23821 gagggagaag actatggcaa ttaaagaaag aaaatttagg gaagtgggga actcagtagt
    23881 attaactatt tcaaaggagt ttcttaaaga aaatgggtta aatcccggag atacggtttt
    23941 tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa caagccttat ctgatgaaat
    24001 tgatttagca atatctcaag cattaactga atatgatact acttttagag agcttgtcga
    24061 taaatgagta ttcattacct agaagccaaa gatattataa aaatgaatgt aatccaaata
    24121 aaaaaatact ctcctaatga accccttggt gtaaaggatg cgaatgcttt agaaatgtgt
    24181 gtagggcaac ttcgagcctc agcttttggt gaagaggttt atccttcgat ttatgaaaaa
    24241 gcagctatcc tacttataca attgataaag aaacatcctt ttcacaatgc aaataaacgt
    24301 actgctttct tagctacttt tgtctttttg aaaatcaatg gacagctttt agctattgat
    24361 caaaaagatg ctgtaaattt agcggtatat attgctacgt atgataagga tttcgatcaa
    24421 ctaaaatatg aagtgatcaa cactataaag acacatacgc agcccattta aggagttact
    24481 gtatgtgtct ttatagggtt attagctgct atagcgaaat aattgaagta aattacaaat
    24541 agctataatt caaatccaac tatatagaaa acgtaaattt tctatatact tggatatgct
    24601 ggaatgttta agagaataat tacatttcta gaaaatacaa taaaaattac aggataatta
    24661 gccatatcct acctcctcta gcagttgctc aaaagttagc actaagtaac ttcttttttg
    24721 ttgctggtaa caatttatca atactaaaat agcgtttaag tatctaataa atactaattt
    24781 taaagctctt aattattatg aaacacgata taacaatatt gctatatcgt gctattgtaa
    24841 tattatacat ttgaagacag cgtattcttg ttctaatatc aggaattttt tataaatatc
    24901 agtcatatta agactcaaaa tttattggta tgttcctctg tatagagttt gaccgtctac
    24961 cattatatag caattattat cattgttgtc ttgcaccaat ataccgttaa gggtagcact
    25021 aggagtagta acaatcactg gaaactcatg agaaagctcg gtcatttttc ctgttctgct
    25081 agaatcgtgt tctattgtca agccatttac tataaacgtg aatactttac ttcctattgt
    25141 tatagtagct ttttgagaaa cagtttctgt agctggagct gtgcttcttt ccgaatttgt
    25201 tagggtttca ctggattcat tgctataaac aattgtagag gattctacgg cttcttgagt
    25261 gtccacgttg gtgctgctag cttcatctag cttccttttg tattgttcgt tactgattgc
    25321 taagctttcc cgttggtcat caagcttatt catttctgta atggtataaa ataatcctcc
    25381 tgctagtgct atattaacaa ctaaactaac cgcaaataat acctttttca ttcttccttc
    25441 tcttctttct ctttttgtaa ttccttactt ttggataggc tacaattaac tagacagaaa
    25501 aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt ctaagagaac
    25561 acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact tag
//
LOCUS       DAFCLG010000027        25363 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFCLG010000027 DAFCLG010000000
VERSION     DAFCLG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.027"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(137..514)
                     /locus_tag="LND56_001078"
     CDS             complement(137..514)
                     /locus_tag="LND56_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743016.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            770..1498
                     /locus_tag="LND56_001079"
     CDS             770..1498
                     /locus_tag="LND56_001079"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK6743017.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1498..1752
                     /gene="purS"
                     /locus_tag="LND56_001080"
     CDS             1498..1752
                     /gene="purS"
                     /locus_tag="LND56_001080"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBK6743018.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1754..2425
                     /gene="purQ"
                     /locus_tag="LND56_001081"
     CDS             1754..2425
                     /gene="purQ"
                     /locus_tag="LND56_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBK6743019.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2426..4648
                     /gene="purL"
                     /locus_tag="LND56_001082"
     CDS             2426..4648
                     /gene="purL"
                     /locus_tag="LND56_001082"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBK6743020.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4633..6072
                     /locus_tag="LND56_001083"
     CDS             4633..6072
                     /locus_tag="LND56_001083"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK6743021.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6095..7147
                     /locus_tag="LND56_001084"
     CDS             6095..7147
                     /locus_tag="LND56_001084"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK6743022.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7144..7722
                     /gene="purN"
                     /locus_tag="LND56_001085"
     CDS             7144..7722
                     /gene="purN"
                     /locus_tag="LND56_001085"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK6743023.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7719..9254
                     /gene="purH"
                     /locus_tag="LND56_001086"
     CDS             7719..9254
                     /gene="purH"
                     /locus_tag="LND56_001086"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK6743024.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9270..10544
                     /gene="purD"
                     /locus_tag="LND56_001087"
     CDS             9270..10544
                     /gene="purD"
                     /locus_tag="LND56_001087"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK6743025.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10666..11580
                     /locus_tag="LND56_001088"
     CDS             10666..11580
                     /locus_tag="LND56_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6743026.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11713..13278)
                     /locus_tag="LND56_001089"
     CDS             complement(11713..13278)
                     /locus_tag="LND56_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6743027.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13271..14722)
                     /locus_tag="LND56_001090"
     CDS             complement(13271..14722)
                     /locus_tag="LND56_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6743028.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14736..15752)
                     /locus_tag="LND56_001091"
     CDS             complement(14736..15752)
                     /locus_tag="LND56_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6743029.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15972..17060
                     /locus_tag="LND56_001092"
     CDS             15972..17060
                     /locus_tag="LND56_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBK6743030.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17114..18640
                     /locus_tag="LND56_001093"
     CDS             17114..18640
                     /locus_tag="LND56_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK6743031.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18642..19847
                     /locus_tag="LND56_001094"
     CDS             18642..19847
                     /locus_tag="LND56_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6743032.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20104..21081
                     /gene="guaC"
                     /locus_tag="LND56_001095"
     CDS             20104..21081
                     /gene="guaC"
                     /locus_tag="LND56_001095"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK6743033.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21345..22769
                     /locus_tag="LND56_001096"
     CDS             21345..22769
                     /locus_tag="LND56_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6743034.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23230..24339
                     /gene="pdhA"
                     /locus_tag="LND56_001097"
     CDS             23230..24339
                     /gene="pdhA"
                     /locus_tag="LND56_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBK6743035.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24342..25319
                     /locus_tag="LND56_001098"
     CDS             24342..25319
                     /locus_tag="LND56_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBK6743036.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8205 a         4317 c         5696 g         7145 t
ORIGIN      
        1 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc
       61 ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat
      121 acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat
      181 ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga
      241 tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt
      301 cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg
      361 ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt
      421 ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata
      481 aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta
      541 tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga
      601 tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat
      661 gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa
      721 caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa
      781 agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat
      841 ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag
      901 tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag
      961 aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt
     1021 acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg
     1081 tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct actataagga
     1141 tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa
     1201 gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga
     1261 attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa
     1321 tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg
     1381 tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt
     1441 ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg
     1501 tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca
     1561 gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa
     1621 tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt
     1681 tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg
     1741 gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga
     1801 tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga
     1861 gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta
     1921 tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc
     1981 tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg
     2041 acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc
     2101 attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta
     2161 tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact
     2221 aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa
     2281 cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg
     2341 tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa
     2401 aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat
     2461 caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga
     2521 agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg
     2581 gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg
     2641 acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact
     2701 agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg
     2761 agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat
     2821 tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga aatatttact
     2881 agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg
     2941 cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt
     3001 aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat
     3061 catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga
     3121 agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat
     3181 ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat
     3241 aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa
     3301 agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac
     3361 accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg
     3421 agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg
     3481 taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct
     3541 gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc
     3601 tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca
     3661 agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac
     3721 ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat
     3781 gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct
     3841 gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat
     3901 tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa
     3961 accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac
     4021 gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc
     4081 aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac
     4141 tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga
     4201 ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa
     4261 ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg
     4321 attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc
     4381 agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt
     4441 tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga
     4501 agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga
     4561 aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga
     4621 ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt
     4681 gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat
     4741 cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga
     4801 gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga
     4861 gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt
     4921 cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg
     4981 atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac
     5041 tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat
     5101 aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa
     5161 acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg
     5221 aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt
     5281 tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt
     5341 gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc
     5401 agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg
     5461 aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct
     5521 ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta
     5581 aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt
     5641 gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc
     5701 gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca
     5761 ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac
     5821 gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc
     5881 aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg
     5941 atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat
     6001 tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta
     6061 tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc
     6121 aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt
     6181 caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga
     6241 tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac
     6301 gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc
     6361 gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt
     6421 agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg
     6481 atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta
     6541 ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt
     6601 attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat
     6661 tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt
     6721 atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa
     6781 aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca
     6841 tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc
     6901 acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg
     6961 tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc
     7021 agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt
     7081 ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga
     7141 acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat
     7201 tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa
     7261 gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa
     7321 gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag
     7381 atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat
     7441 tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat
     7501 ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc
     7561 gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag
     7621 gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa
     7681 gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg
     7741 tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa
     7801 tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga
     7861 tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta
     7921 aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa
     7981 cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga
     8041 ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta
     8101 gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg
     8161 actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac
     8221 aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat
     8281 acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa
     8341 aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc
     8401 ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata
     8461 ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg
     8521 tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag
     8581 cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga
     8641 atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca
     8701 ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat
     8761 tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct
     8821 taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag
     8881 tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag
     8941 ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa
     9001 tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag
     9061 agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg
     9121 aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc
     9181 aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc
     9241 attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc
     9301 gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg
     9361 cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag
     9421 atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc
     9481 ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct
     9541 tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa
     9601 tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag
     9661 cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg
     9721 ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt
     9781 tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag
     9841 gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt
     9901 cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg
     9961 cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt
    10021 taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg
    10081 cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg
    10141 atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg
    10201 atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg
    10261 ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt
    10321 ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag
    10381 ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac
    10441 agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag
    10501 atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt
    10561 tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag
    10621 agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa
    10681 ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat
    10741 gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac
    10801 catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa
    10861 acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg
    10921 atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg
    10981 aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag
    11041 gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc
    11101 agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta
    11161 gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc
    11221 aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg
    11281 catgatatga cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt
    11341 atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat
    11401 caaacgacac caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa
    11461 gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt
    11521 aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa
    11581 agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct
    11641 gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac
    11701 ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg
    11761 agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat
    11821 ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc
    11881 gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt
    11941 actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg
    12001 atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt
    12061 ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg
    12121 gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat
    12181 ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa
    12241 taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta
    12301 cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac
    12361 agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt
    12421 aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct
    12481 gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa
    12541 ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga
    12601 atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt
    12661 ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact
    12721 gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga
    12781 ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa
    12841 atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa
    12901 atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt
    12961 aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa
    13021 tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt
    13081 caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat
    13141 aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga ttggataata
    13201 agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat
    13261 gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca
    13321 gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc
    13381 cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg
    13441 aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa
    13501 tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa
    13561 taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct
    13621 gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt
    13681 caagcatatg gccgtatctt atttttaaga tgactagata atcattgata tggctaattt
    13741 ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa
    13801 gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg
    13861 catccaaact gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac
    13921 tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg
    13981 ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac
    14041 gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt
    14101 cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa
    14161 gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt
    14221 tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag
    14281 tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga
    14341 tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat
    14401 ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc
    14461 taagttcttc taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca
    14521 agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt
    14581 ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca
    14641 gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt
    14701 ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta
    14761 gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt
    14821 cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca
    14881 ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct
    14941 tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc
    15001 gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat
    15061 aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca
    15121 ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt
    15181 agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg
    15241 gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag
    15301 tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg
    15361 gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc
    15421 cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa
    15481 atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga
    15541 ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt
    15601 gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact
    15661 agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca
    15721 acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag
    15781 tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc
    15841 aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc
    15901 ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg
    15961 ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg
    16021 caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg
    16081 taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg
    16141 tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag
    16201 aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg
    16261 cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca
    16321 aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt
    16381 ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga
    16441 aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg
    16501 cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt
    16561 tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt
    16621 ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg
    16681 atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg
    16741 tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg
    16801 ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta
    16861 tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg
    16921 tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata
    16981 cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag
    17041 aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga
    17101 gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc
    17161 tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt
    17221 gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc
    17281 atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt
    17341 gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta
    17401 tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga
    17461 ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga
    17521 cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa
    17581 agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga
    17641 agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc
    17701 catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt
    17761 acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat
    17821 tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg
    17881 agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat
    17941 cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact
    18001 gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa
    18061 gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc
    18121 cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct
    18181 gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt
    18241 attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa
    18301 aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt
    18361 tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg
    18421 gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg
    18481 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
    18541 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
    18601 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
    18661 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
    18721 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
    18781 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
    18841 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
    18901 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
    18961 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
    19021 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
    19081 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
    19141 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
    19201 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
    19261 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
    19321 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
    19381 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
    19441 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
    19501 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
    19561 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
    19621 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
    19681 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
    19741 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
    19801 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
    19861 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
    19921 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
    19981 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
    20041 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
    20101 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
    20161 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
    20221 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
    20281 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
    20341 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
    20401 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
    20461 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
    20521 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
    20581 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
    20641 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
    20701 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
    20761 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
    20821 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
    20881 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
    20941 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
    21001 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
    21061 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
    21121 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
    21181 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
    21241 agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt
    21301 cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag
    21361 agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa
    21421 acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg
    21481 gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa
    21541 cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc
    21601 caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc
    21661 aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg
    21721 agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct
    21781 tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac
    21841 ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg
    21901 gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag
    21961 ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt
    22021 tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc
    22081 ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag
    22141 tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa
    22201 ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta
    22261 tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa
    22321 aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag
    22381 cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat
    22441 ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc
    22501 gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg
    22561 gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag
    22621 tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa
    22681 tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga
    22741 aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa
    22801 cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag
    22861 aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt
    22921 tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat
    22981 tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca
    23041 ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt
    23101 agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac
    23161 agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat
    23221 gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc
    23281 aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat
    23341 ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct
    23401 cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca
    23461 ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat
    23521 gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct
    23581 caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa
    23641 gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta
    23701 ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca
    23761 tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta
    23821 ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca
    23881 aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg
    23941 gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat
    24001 ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact atctggtgac
    24061 gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg
    24121 atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa
    24181 gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca
    24241 aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa
    24301 gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga
    24361 tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt
    24421 ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa
    24481 agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga
    24541 gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta
    24601 ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg
    24661 gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag
    24721 aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga
    24781 ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc
    24841 cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg
    24901 aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta
    24961 ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag
    25021 aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga
    25081 tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag
    25141 cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag
    25201 ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg
    25261 tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag
    25321 aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DAFCLG010000028        24748 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFCLG010000028 DAFCLG010000000
VERSION     DAFCLG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.028"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..173)
                     /locus_tag="LND56_001099"
     CDS             complement(57..173)
                     /locus_tag="LND56_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6743037.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..1332)
                     /locus_tag="LND56_001100"
     CDS             complement(418..1332)
                     /locus_tag="LND56_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6743038.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1347..2348)
                     /locus_tag="LND56_001101"
     CDS             complement(1347..2348)
                     /locus_tag="LND56_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6743039.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(2345..4528)
                     /locus_tag="LND56_001102"
     CDS             complement(2345..4528)
                     /locus_tag="LND56_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6743040.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4531..6978)
                     /locus_tag="LND56_001103"
     CDS             complement(4531..6978)
                     /locus_tag="LND56_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6743041.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6962..7354)
                     /locus_tag="LND56_001104"
     CDS             complement(6962..7354)
                     /locus_tag="LND56_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6743042.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7441..7938)
                     /locus_tag="LND56_001105"
     CDS             complement(7441..7938)
                     /locus_tag="LND56_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6743043.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(8055..8279)
                     /locus_tag="LND56_001106"
     CDS             complement(8055..8279)
                     /locus_tag="LND56_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743044.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="LND56_001107"
     CDS             complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="LND56_001107"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6743045.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11077..12282)
                     /locus_tag="LND56_001108"
     CDS             complement(11077..12282)
                     /locus_tag="LND56_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6743046.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(12305..12458)
                     /locus_tag="LND56_001109"
                     /pseudo
     CDS             complement(12305..12458)
                     /locus_tag="LND56_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12461..13846)
                     /locus_tag="LND56_001110"
     CDS             complement(12461..13846)
                     /locus_tag="LND56_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6743047.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13875..14261)
                     /locus_tag="LND56_001111"
     CDS             complement(13875..14261)
                     /locus_tag="LND56_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6743048.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14277..14591)
                     /locus_tag="LND56_001112"
     CDS             complement(14277..14591)
                     /locus_tag="LND56_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6743049.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="LND56_001113"
     CDS             complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="LND56_001113"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK6743050.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15851..17140
                     /locus_tag="LND56_001114"
     CDS             15851..17140
                     /locus_tag="LND56_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK6743051.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17179..17784)
                     /locus_tag="LND56_001115"
     CDS             complement(17179..17784)
                     /locus_tag="LND56_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBK6743052.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17980..18444)
                     /locus_tag="LND56_001116"
     CDS             complement(17980..18444)
                     /locus_tag="LND56_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6743053.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18466..18786)
                     /locus_tag="LND56_001117"
     CDS             complement(18466..18786)
                     /locus_tag="LND56_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6743054.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="LND56_001118"
     CDS             complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="LND56_001118"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBK6743055.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20208..20558
                     /locus_tag="LND56_001119"
     CDS             20208..20558
                     /locus_tag="LND56_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6743056.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            20743..21696
                     /locus_tag="LND56_001120"
     CDS             20743..21696
                     /locus_tag="LND56_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6743057.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            21709..23496
                     /locus_tag="LND56_001121"
     CDS             21709..23496
                     /locus_tag="LND56_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743058.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            23483..24685
                     /locus_tag="LND56_001122"
     CDS             23483..24685
                     /locus_tag="LND56_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743059.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6992 a         5007 c         4518 g         8231 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgctttg gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc
      541 cttacgattg taaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt
      601 taatatcccg tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt
      661 gaagaacgtc gttaaaaact cattgatttc attggttgta atggaatcaa ccgtaccctc
      721 actttcaata gcttttggtt tatagtctga tttctttggt atgctagtaa tggtcggatt
      781 cttaatgagg accatatttc cagtactgtc tacatagaca ctcactatat aagcagagtg
      841 gacggtcttt gtattttccc cctctgtaat gagttggtct acactgtagg ttacatcaaa
      901 ttgattgtcg ccatctgctt ctaccgtcca tatctgaaat ccttttacag aagacgatac
      961 aggaatatct ttacgaactg tatcgacatt cagagcttga agttcctctg tcagatatgc
     1021 tttcaggttt tctatgcgat tatcaatgga cttgtcgttt agttcccatg aatagtagac
     1081 tttcgcaaag ttctctacaa aattttccac atgatgagta tcgacatatt ccttttctat
     1141 gatggtggtt tcatgaatgg tatgggtatc aatggctgta aagtgcttga atatcgcaaa
     1201 gctgaaacta agtcctaaaa gtacccatag gacaatcaca acctttttac gaggattgac
     1261 cttatagaca cgaggtttct tttcctttgg tgtctgtttt tctgtattct gattttttcc
     1321 gaatttcatc attgaatcgt cctttcttat tgtttgattc gtcctgctcc cactaaatgc
     1381 tgttgccagt aagagcttgt taagtcggca taaccgatag ggtcgcctgc atgaaacata
     1441 cggttatcgc ctaaataaat cccgatatga gtaatataag agccagcgtt ataggtagaa
     1501 tgaaagaaaa ccaaatcgcc ggcttttgct tccaataatg ggatatgccg ggttacatca
     1561 tattgctgtt gtgctgttcg tggtaagttg attccagctt ttccatacgt ccattgtgtc
     1621 agtccgctac aatcaaaaga ggtagtcggg gaagctccgc catagacata cttccagcct
     1681 tcaaatttca gagcttcgtc catgatggct tgtactgtat catcatcaaa ctctgttgtg
     1741 acaagatact gcgttaccag ttgcacataa aacatattcc cgtaattgta tcgccagccc
     1801 ccattgatag gtatggctat gggattgggg taagacactt tttcgtcacc cgaatactct
     1861 tttgagaaac tttgagccag ttcaaaggta tatttattcc cacgattagc cacatatcct
     1921 aagaaaccac caccataatt gtaggactgg ataaccgatt ctaaatctac accaagcctt
     1981 tcgctactgg ctaataactc actgaaatac ttcacacctt gcttaatgga ttcttctgta
     2041 ctcaatgaat taggtggaag accgagggat tccgaggact gcataacatc ttctgctgta
     2101 ccacccgatt ccacctgtat aatcgcaaga agtatattga cgtattcttc aattccatat
     2161 tctttggcaa ctttttctac cataggctta tgagccagca cttctgcgga aacatccaca
     2221 ccgccatagt gaatgttgga aaaaccgcca tcttgttcat ctgaaaataa aatggcgaca
     2281 aacagaagca gtgagaagac catcaagaat aatcccgaac caccaatcac taaagttttc
     2341 agcttcatgg tttcttactg cctttcttaa tggtggcggt tttgattggt ggtctacttt
     2401 ttgtattttg tagtggtact ctttgaacag tagactgacg ttcttttgtg attgaacgtt
     2461 gtgaagctct atctgttggt gcagttgaag ttactggctt ttgaaccgtt ctttcttgaa
     2521 ctgtattact ttgacgttcc gtttttaaac ttgaagaatc ggacttaact gttggacgct
     2581 cttgtttggc ttgttgagat tccctatagg aagcctgtac agttgtttgc cttgaagtct
     2641 gtccatcatg agattgttct tgcttagtgg ctggtctttc atgaacggaa gaagctggct
     2701 gttttttctg tttgacctgt tccatttcag agcgacgctc cgcaatggtt ttccgtcttt
     2761 gttcctgctg tttcttgcgt ccactggctt tatccgcttt ggtttgagaa atacttctgg
     2821 ttaaatcatg gacattatcc gtcactttag attttccttg atatactgca tatcttgcat
     2881 tggtcggcaa atccttaacc tgttctttca aattaccagc agtatctacc attctgtctt
     2941 tggtatcagc tactgtaccg atggtttgac cgatacgctt tcctagtgtt gatttttcct
     3001 gcccgtttgg tcgagagtga tctgcttgtg tactcgcaga acttcccgaa cccgactgtc
     3061 cttttttacc cgtagcactg gcaatggcag acccagcccc taaagcagtc acggagcgtc
     3121 caagtttccg ctgtagacgg tgcatgtgag cgtgcataag catacgaggt tttctcatca
     3181 tacgacttcc cacactttga gaatcgttac tctgtagaga aaacatactc attaaatcgc
     3241 ccagcttaaa gtagattcct gcaaaggtca caatctgtag aaaagcaatc aaaaagaacg
     3301 gataaccagc cgataaggta tagagcatgg ttgaaatact aaatgctgtc gtaataatca
     3361 atgtgattcc agctcgtgtc aaaatggtat taaagagctt tgttatggct cgttttgaca
     3421 taccatcaaa tgatggaatc atgcttaaaa taaagctcac aggcagaaac atagcataga
     3481 tgataaaaag tacctgcgag aaaatcatga ttcctgttaa taggaataca aatatggaaa
     3541 tcccaatatt gaagacaaat aggaagaaga ctgtacctaa acggttaatc gtctttgtaa
     3601 tcgtcatatt ggtattgctt ctatcttcaa tttcttccgc aacaattttt tctctgtctt
     3661 ccccattgtt ggaatctgga ctggttgaga gcaggctttc cacacggtca ataccaatac
     3721 tttcaatgtc tgaactgtta tattgaagca gtagccacgg ttgctgaacc tgtatggaaa
     3781 ataggctgtc acgtatcaag tccacactgt ccttgccttg actatctgaa tggggcatga
     3841 caatttttgt acccagtgac aaactagcgt tgctgatgtc tgatgaaaag tcattgattt
     3901 ttttaatgta gtcgggagcg taggcaataa aggaagccga taggataaaa accagcacaa
     3961 aattcatgag ggcatgaatt gcctttgtgg tttctctctt tatcagtcct gtataggcaa
     4021 cataaacccc aagaaccaaa atcaagagta aaaggaatcc aacatagaaa ccctctgttg
     4081 atagtccact aggcgtaacc cctgcaaggg tctgcatatt cttgccaatg gaatctgctg
     4141 tagcggaaat gaagtctaag gaataggctt cctgtactaa gtaacccgtc gcattggaaa
     4201 cataaagact gattgtccaa ataaaattgg taatggcata tagtccatac atgacttgtt
     4261 ttccaattcc gtccgaccag ttccacggta gccagcccca gctattatcc acataaaaat
     4321 ccagttgata gttttcaagt gggtatcggc tgtattcatt tgccacattg accgtatcat
     4381 ctaccaagcc cgcagcttga accaccgttc ccagcatggc taaaagaaaa atggcaatca
     4441 caagtgtgaa agccactgtc attgccactt tacctagacg tttcagcgtc cagtttgatt
     4501 ttattctgtt taatattgat ggtttcacat ttacacctct tttcgcacag gtggtctggt
     4561 atcaaaggca tggagcagtt cttcaaatac agggtggaac tgtatcacac cgacacgacc
     4621 atataaatca ctgataaggc attgcccgtt ttccaaatca cgcaatcgct tctgattgtt
     4681 ttcgtcctct gggtctacac caaaaaaggc aagggttttt ttaatctcgt taaggtcagt
     4741 ggaacgaaag gcaaatttta agccgaggtt atttttcagt ttttcatcta agaggtcatc
     4801 tgtattttgg gtcacgaaat ataccccagc gttcatagca cgaccagcac gaaccagctt
     4861 catagatagt gttttccctt gtgccacctg taagaagctc catgcttcgt ctaagtctac
     4921 aatcttgaaa atacttcggt ctgtatggat aaagtccaac gcaaaggtac taatgacaat
     4981 cagcattgct accgatagta actccattgt ggtatattcc tcaaaggaag tttccttgtc
     5041 aggaagtacc aagtcggcaa cctgtataat gttcagttgt ttttcaaggc tgatagactg
     5101 ctctacataa ccatcactaa aaagcagatg ggcaaagtca tagtctgtaa aactttcgat
     5161 atggtcggct atgctggtac ttagtggcgt attctcaacc cgtaattctt caatcacttt
     5221 catcaaccct cgcacttcac tattggttac tgcacgaatg gcttttctaa ggattgggaa
     5281 gcgttcccca tcacgagagg aaatccccgt aaggaatgtc aaaatatcaa tagccagtga
     5341 ttcagaatct ttggtatttt tcataattac ataagggtca agtaagcctt tgtttttctc
     5401 atcagaagtc agattgacga tattgatttc atgggaaatc tctggcaagg tttctttcca
     5461 tctgccacgt tctgcttttg ggtctacaat cactgcttgt gccccataaa gcactgcata
     5521 atagacgata aggttattcg caaaggattt accaccaccc agcgaaccga caaaggcaga
     5581 cgctaacgca ttggttactg aacccttaac cccttgactg gcaagagcag gtttcagata
     5641 gacattgcgt ccagtatcta agctgtagcc aacataaatc ccctcatttt cacccagcat
     5701 ttgagtagca ccaaaaccta aaccagcgag gaaatcagag gtcacgtatt gaatatagtc
     5761 attcatatat cttttgctgg caggtaaaaa ttcttcatgt aagcccagca tatccccaaa
     5821 aggtcgtacc agtttcacgc tcaaatcatc atagaaatct ttcacttcat tacaacgacg
     5881 tttaagttcg tcaaggtcat ttgctgatac ccttacaaca taggacagct tatacataga
     5941 ttccttgctt tggtctaaat tggtttccag ctcgttcaca ctttccagag cttccgccac
     6001 attagagctg gtttcattat cactttgcca agcgtggtta tccaagtctt tcagttcttt
     6061 ctttttattg cggacagtag atagggcttt acgattcgct acaatttcca cattcattga
     6121 cgtatcaatc ggaaaggtaa attgctgttg ctggtagtag aagatttcag aggacgggaa
     6181 gtccagttcg ccgacaatac tgttgatggt aaagtaagct acataggcgg tttcgtcttc
     6241 ctgctggatt ttcaaatatc gctgtttttc ttccaccaga cagcgagtag gcttaatgag
     6301 gtcataggtt ttaatcagtg tttcatgttc cagctttttc tttgatagag gatactcata
     6361 ctcttcatag gcagtgcctg tctgtccgta aaggtgttca atcagatagc cgaagtcatc
     6421 cttatctaat cttcggattt tgaaacgacg ggagatttta ttttctaaaa gcttttccat
     6481 cttctgaaaa cgcaggattt catcattact catactaaca aaatcgccca tcagcttatg
     6541 gttcacatca tagacaaaat cagtaaaagc attttttgct tcaacggtaa gacttttcat
     6601 agaaaactcc tgatcgttga gaagcaactt aaaaccgata aagaaacggt agtccacttg
     6661 attttcgcca atcatggata ttaaagcgtc tgtctgttgg tcgattttgt cataggcaac
     6721 cgttttgagc tttccagtga cttcattttt ggaacgttct tgtgcagaaa gtatgctgga
     6781 ttctgtactg atttgtaaag catgaatctt gccatcacga ttttgtgcga taagctgtct
     6841 gaaagaatca tgtacttgta ttttctgttc cggacttaga aatgagtaat tgtagggaac
     6901 aagctcatag taagcgtaac attccccatc tttattccag accagattgt tttcaatata
     6961 tttaattgga tatgccataa aattcactcc taactgctgt aatggcttct tgtggctggt
     7021 ttctgccaag cgttactttt tttcctgcat aggtcagctt tcgtcgcagt gcataagcaa
     7081 tgacagactt caaaaatcca taaggctttt taccatcaaa ggtttttgta gacataaacc
     7141 atgtgaaagc cacaggaatc ccaaagtatt tgagaaatgc tccctctatc atagaaagag
     7201 ggggcaagtt gccaagtatc ataactgcaa agagtgacac gacaaaccat gtcatttgcg
     7261 taaaggttat gggaaacgga agtctaaagt cattgatgga gtacaacact ttctcaacag
     7321 accagatact ggtatagctt cgtattttct tcatgtaatc aatcctttca taaaaatggg
     7381 gatagtcttt tgactacccc gtaaacaaga aaatctgcca gtaagaatgt accgacagat
     7441 ttaatagacg atttcaaaaa tcccgtgatt ggttgagata aacgttcccg aaaggtctaa
     7501 atcccgacca taggattcat aatcaatata gttttgaaga ctggctggca cttcgcctaa
     7561 agcacccgtt tcttcaatgt agtagcgtgc cacgtcagac atatcatcac aatcggaatg
     7621 aatgataata tcttcttgat gttcgcttag ttcttcaata cttgaaaaat gagtgagcag
     7681 agcagatagc tccgattgta gttcttctgg caactccgat accatttccc ataggcgatt
     7741 gagttcgcta atagaagtgt attcgtcaac cgtaaagggt aactcgtaat catgaatggc
     7801 gtattcctca tattcatcat tcaagccgat tttctctttg acttcctcaa agtcaatggg
     7861 aaaggtaaac cacgcaccga ccaattcgcc ctcattgtat ttgcctaaat tcgcaatata
     7921 gacttgcata tcgtccatat attcacgtcc tttctttgta gagattcaaa aatccctacc
     7981 gcacttcgtt tggtgtacca ttcctttgcg gaacataaga aaaccactta tattccacaa
     8041 aagaacggtt ttatttaagc accaataatg cgattgaata gctctagtaa aatgtctttt
     8101 actccagcag cgttgaagac taagccaacc gcaataatcg caataattaa aaagccaatc
     8161 agtttgctaa actcacgctt gaagccaaga tacaagccaa tcacaacgat tgctaaaagc
     8221 accagtgatt gagcgtttga tagaaaccag ttatatatgg ataggtaagt tcctgatatt
     8281 ataaatatct caaacttttc cccccattca cacggagcgt gcgactttca ccgcactccg
     8341 cgttccatcg tgtttatatt gaaaagtatt tatgtagtag acctatatca agtttaattc
     8401 ttgattgcca gctaatactc tatatttatt tagtcttatg agctggactt tatttagctt
     8461 caatagagct atatattttt gtattgtttc ttctgtttta gcgtgtatta gacggtgtac
     8521 cgcctctttt accaaaataa gatttttata atcgtcattt ccaccagctc cagttggtac
     8581 aatatgatga caatgtattt cattagcttg ttgaaattct tccagagtta tagcacattt
     8641 tccatgttga gcagaaaata gcgaaattcg attgtctgcg tattctattg tgcgattttc
     8701 caaagataat ttcattaact ggtacatcag taatttatca acacttagat tggtgtggat
     8761 aaattttctt ccctctagag tgtagcaatt cgctaaatac acctttgcca ttggttttct
     8821 gtgttggata tatccaacag ggaaaatagg ttcgccctcg ccagcaacat accgaagcat
     8881 tttagattta ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa
     8941 tctgcccctt ttgtttgtac ctaatctgtt agtgaagata gccattacag ctctattcag
     9001 tttcatacaa tccagattta cattcgttgc aatctgataa taattttgca tacctaatac
     9061 cattgaatta taaagtcttg tttcttctaa ttccgttttc ccttttgagg gagaagcaat
     9121 tttctttgcc tgtgatacca gcttgtcttt ttggtttttg aattgtttat cacttatatg
     9181 tgatttaaca actaacttct ttcttctcgg tatcatttta atcttgaaac caagaaagtc
     9241 ggagtatctt tttctaacat ttacaattct tgttttttct tgtgatactt ccaattttaa
     9301 tcgttgttca atccattgag tgattgctat tttggctttt tcagcacttt ccttgtagcg
     9361 gcagaaaact ctaaaatcat ctgcataacg aaccatatac atttccttta actttgtttt
     9421 cttcatttct ttgtaagcgt tgcttttaca tgggctaccg cttttattga tatgaaccac
     9481 atagtttttt gtaacaggat tttcctgcca ttggctttct acccaatggt ctaactcatt
     9541 aagaacaatg tttgctagta gtggggatat tattccccct tgcggagttc ctttctctgg
     9601 atatgttatt gtaccgttag tcattttgat tggagctttt aatatccttt ttaaaatgaa
     9661 tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt tttatcagct tggaatggtt
     9721 cacgttgtca aagaaaccct taatatcaaa ttcaatcaca taatgtagct ggctgatttg
     9781 taggcggtta taggttgctt ttattgcatt ttcaactgac ctattaggtc taaaaccata
     9841 gctgttttca ctaaatttag cttcgcagat tggttctaat acttgtttta tacattgttg
     9901 tataagcctg tcccacatac aaggaatacc tagaggtctg gttttaccat ttggttttgg
     9961 tatttctttt cgcctcactg gcttaggtcg atacccatgt ggactgcctg caacaatgta
    10021 tcttactttt ttgacaactg tttctgctgg caatttgcca atatctttaa ttattgtacc
    10081 gtctgttcct gctgttttac tgcctgcatt tgctttgata tttcgatatg ccagtaatat
    10141 attttctctc gataggataa tatccataag atgagtgaaa atatctccat tcttactttt
    10201 ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc
    10261 tacacacaat gtttcatttt tatttttagg cataaggctt cactccttta cttgaaattg
    10321 agtgagcttt tttatcttac tcgtaatcct tattgaaaca acataatact tttctaatat
    10381 atacacgact tgagggcatt cctccattct cattacaaga atttcttagg ttcgccctct
    10441 gcttttcagc attagttcaa ctgcttattg ttcttcgtca gtttattctc ttgatgagaa
    10501 cccaatacct ttcctcgttc cgataatctt atctatacat atatatcctt aggtcactag
    10561 ctataagcct gctggcttga taccgcctgt aacgttatat ggcatttcat aacctatatt
    10621 cttactttgc cacaccagct acgatttctc gtacctttgc atttctacaa agttcaaatt
    10681 tagacccgta cattcgctgt ttcgtcagtt ccttatagaa cattctaacc atagatattt
    10741 tatagacccc cgacctgttg gatacgctac ccacctctgg atagttttcg atagaagata
    10801 tatgttcatc aactattacg gtatctttca gccgtcttta ccgagcttca aacattctga
    10861 gtttcctcat acaatgcttg tcggaggctc agggcaggcg tttcagagcg ttacttcatc
    10921 attccacctg tcagattatc agttctccta ataaccaaac tttttcgttc ttttattagt
    10981 gccttagctt ttcactaagg aacgagtcgc acaaaggttt tgtccaaaat tcataaaaat
    11041 gttctcctct ctatattcaa tgaatttgta tttgagttat ttttttgttg ttatcacgtc
    11101 ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt cggtctgtca gtttcgcatg
    11161 gtcgagaatg tcttttacaa cctgcgtctg gttgatttca tcaagtttaa tcgcaacctt
    11221 taaggtcggg gcaacttgat gagatagcca gttcagcgtc ctttggaagg agtaaggctc
    11281 tggttttgtg gttagtttta atcgttcacg attgttccca ataaaccaag cccattcttc
    11341 attcagtttc caatcagaac gaggtttgga atcgtcttta tctacaaaac ggatataccg
    11401 attgataatt ttaaaggcgg tatgctctgg attgtcatag acgagtaaat cacggactgc
    11461 ataataggca cgctcatttt tcaatcgaat ctcaaaacgg ttttttactt ctgcgtcttc
    11521 aatgggaata tcatttttct tgtactgctc gtagtccttt tcatagatac agaaataaac
    11581 ttcactttgt aatgaaccga tatagagggt gtttcccata cattcctttt cctctttgcg
    11641 taccagttcg ccactgcgat agcttttaaa actgcggaag acggagatac attcttcctg
    11701 ttggcacttt tcagtgagta cagggatatt taaaatccct gtcttatcgt taatggcaag
    11761 gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc ataaagaact cataccagct
    11821 tctttgttgt gccagaagat agctttcaaa ttgtctgcac ccacgacctt tcaattccac
    11881 cagaactcct ttgtccagtt catgggagca aaggacgaat atgtcgccta aagcataatg
    11941 ctctgaataa gaatagaaac catagtcctc atgaagaaaa taggacagtt tcagttgtaa
    12001 gatgttttcg accacctgct gtacgtctgt tgtcggaaag cgaattctta cataatcaaa
    12061 cagcatttca aggggagcgt cgggattgaa gcgttccaga gcttcccaaa gggactgctg
    12121 taaatcctct gatggcttga cttttcctgt ttcaatatcg cttagatact gccttgtaat
    12181 accagtcgca acagctaaac ggttttgaga tagtccataa gccaagcgtt tttcttttaa
    12241 atgctgtaac caagtttgtt cattcagtaa aaatccctcc aatcaaaaag gcgtatgtca
    12301 acttttaaag cccatttgac atacgctgaa attttgtaaa tcccttgtaa ccaaaggatt
    12361 ttctaatgtt tttttgactg tttcctgtcg atttgtaccc ccctgttaga taccgagggg
    12421 ttaagtgctg gcgtggctat tgccacacca gccagcaaga tcagtccaca cctgcgactt
    12481 ccgcttcgca cgtcgcctgc gtggactgtc tgctgttgga taacttttta atttcctcca
    12541 agaaatcata tccttttggt acaaggggag tataaaactc tgatatgaca cttgttccta
    12601 catcaacata gccacgacct ttgattcgct ttaagaagaa atccttttgt acgtcactgc
    12661 caaacatcat gccatagccc atttcagaca tacgacctaa agccactctg aaattaaact
    12721 gatcacggat tccgtcgcct aaatattttg cgtctggacg ttgacaagcc agtattagaa
    12781 agaagccagc ttgacgacct aacatgacaa tctgtttcag cttattcata actgcggtgt
    12841 tttcttttgt tcccagcatt tccatgaaag cgacgtattc atcaaagatt aagaagtgtg
    12901 ccgggagacc taagtaagca taatttttgc cagtcttata gttcttcatc tgcttcattt
    12961 cctcactacg tttcatcatt tcttcataga atgtttcaat gcaagaaagc aagtcttctt
    13021 ttctatagta gacatttgcc atcacagaac ctaagtccgc aaggtcagca tttttcgggt
    13081 caagaatata cagttttgaa tctgtatgaa gcaaggcttc aatcagtgtc agtataaagt
    13141 aagttttacc gccacctgta ccaccagcaa tcaacatatg agggagctta tcatattccc
    13201 accatacgtt tttcattaag cgaagtttac catctttagc ttctacttca tcaatagaaa
    13261 tacgactggc tatggtgtca tagagcaaag tatattccac ataggaatcc tttaactctt
    13321 tatccgtcag ctcacagtac aagccactct ctaatttctt ttccaagtgt aagagttggt
    13381 cttgatattt tcccagcgtg atttccaccc gtatctgtat caagccattt ttaagtcgat
    13441 aatacatttt agggaagtag gttatctttt cctttgtacg accagcacta tctttaaaga
    13501 aaccctctgt tttgacctgt tcagattcat accacttgtt ttcaagtatc atctttgcca
    13561 gtttttgacg gtggtaaagt tgtttaaccg tatcatagcg aacccgtttg aatacaaacg
    13621 ctaccagcaa gcagataaga attgcgacac tgaaactgat aattaaatag ggaatgtcaa
    13681 tcttatctgc ttgtgatagg ttaaaatcct gccagttgat ctgctggatt gtcttcacat
    13741 gaaacagtcc gacaaccagc aggaaaacag gcaggagtga cgctatcgta aaatgaaaga
    13801 ctaaatcttt accagatggg cgaatccttt taccacgctg tttcatgcga aaaagtctcc
    13861 tttctaccta gcgactattt gtcttgtgtc ggttctttct ttgcttgtgg ttgagctttg
    13921 aatgaactag aatcctttgt cagcacaata tcgtctgcct tgatatacca gtcaacatct
    13981 gctccttgat aggtggcagt agcaacggtg tccgcaatgg gattgataag ttccacccgt
    14041 gcgttataat caaactcttt caaaggcacg ctggcaggaa tacttacttg aatcatgcgt
    14101 ccttgtcctt tggattttaa gtcataggta cgttccttga tttcatctga aaccgacccg
    14161 tcttcatttt ggattctcac ttcacgacgt agagcagaga atttcaattc tccaaaagtc
    14221 gtgtctttat ctaatacaat gccatttgct aatctcatca tttttcctct ctttctttat
    14281 tcttttatca tgtcgtcagc atgtaaaagg taatttgtaa aaccacgagt gccgattttg
    14341 tagccctctg cggtaatacg tggattgact aacttcacac gttcctcaaa gccgaaatgt
    14401 ttttcgccag cttcagcagg aagcaccacc acaatatcat ctgctctttg aacatcagaa
    14461 tagagattat agcttcttga taagacagtt agccgtccgt tgattcttcg ctgaacgact
    14521 ttatcctcgc cagcaaattc taaattgccg aatgtttttt ccatgttggg aatcacaaat
    14581 ttaagttcca tatttttacc tatcctttct tttttattgg ctgaatgaat gtttgatggt
    14641 cttaaagagt ggggaacgac cttttgattc ttgatttttt gttttcataa gttcacttcc
    14701 tttcaaaatc gggtaaaaaa atagacacct cattttttga agtgtctacc tattaaatat
    14761 tcaaatttta ttggaagtat ctttatatct tcacttttca aggataaatc gtcgtatcaa
    14821 agctcattca taagtagtaa attagtagta aattgagtgg ttttgacctt gataaagtgt
    14881 gataagtcca gtttttatgc ggataactag atttttatgc tatttttctc ttaaaaaata
    14941 tcattatttg tggtatggag cccccgcgtt gatccggaag gcacggtaga tttgctcgac
    15001 gaggatcagg cgcatcaatt gatgtggata cgtcattttg ccaaaagaga tctgagcatt
    15061 gcttcttttc atgacggctt cgctgagtcc taaagaaccg ccgataacaa aaacaaggtg
    15121 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcgc ttgtgggatt
    15181 tttcccttca attgctaagc catagacgta ttcttgttct ttgattttcg ctaagatccg
    15241 ttctccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
    15301 ttcgtcagga acttcgatca attcgatttt cgcataggca ttcagccgtt tgacatactc
    15361 attgatacct tggatcaagt atttttcttt taattttcct actgaaataa ttttgatatt
    15421 catgttttct ctcctttgat cttcacgctc actttgttta ttataatcaa ttttttcgtc
    15481 tatatgttta ttttgactat ttttgttaaa aagatttccc caacagttat gcacaacatc
    15541 cacaggttta tccacagaaa acagcgttgt tttaccttat ttcatcacat tcattaggca
    15601 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
    15661 ttatttttcc aatcctatcc gcgtagtttt acacaatatc tgtggaaaaa gaaaatcaaa
    15721 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt caaagtctca
    15781 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
    15841 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaaata aaaataatag
    15901 cctctggcgt aagttgggtc tggggcttgt tggagggatc gttggaggac tactgacagc
    15961 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
    16021 tcaaaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
    16081 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
    16141 aaaccaatca agcggttttg gcggattatt cgggcagcaa gaagaaagta gcagctcaga
    16201 tgatagtaat ttggaagctt atagtgaagg aagcggcgta atctacaaaa aagacgggaa
    16261 tactgcttat gtagtcacaa acaatcacgt agtagatgga cagcaaggtt tggaagtcat
    16321 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgcct attcagattt
    16381 agcggtgttg aagatcaatt cagacaaagt cgaaacggtt gcctcttttg gggactcaag
    16441 tgctttgaag gttggtgaac ctgcgattgc gattggttcc ccattaggat ctgaatatgc
    16501 gaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtaacaa gtacgaatga
    16561 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcgatca acccgggaaa
    16621 ttctggtggt cctttagtga atatcgaagg acaagttatc ggtatcaact caagtaagat
    16681 cgcaagtact tctgaatcga cttctaatgt aagcgtagaa ggaatgggat ttgccatccc
    16741 aagtaacgat gtagtgaata tcatcaacca actggaaaaa gacggaaaag tgacacgtcc
    16801 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
    16861 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
    16921 accggcagaa aaagctgggt tgaaacaata cgacgtcatt actaagatcg atgacaaaga
    16981 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
    17041 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
    17101 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
    17161 agacttgtca caacctcttt attttggaaa tacttctccg actacggcat cctcaggcaa
    17221 tactaagatt cctttttttt ctggagcatt tggcaaatgc aattcacgag cagaacaaat
    17281 cattccatag ctggctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
    17341 catggctcct ggttttgcaa cgacaacttt ttgtccagct tcgatatttg gtgcaccgca
    17401 aacaatttgc aatactgtcc cattgtccac ttctgtctca gtaatcgata aatggtcaga
    17461 gtcaggatgc gggacgcatg tttttacata cccgacaacg atcttcggtt catgatcagc
    17521 ttcaagctgt tcttcgaatc ctgcttcttt aatcaatcga ttcaatttcg ctaattcttc
    17581 atctgttaag cttacttggc cgttcccttc aattgttaga tgatcggaga tatggaaaaa
    17641 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttacgttc
    17701 gacagtgttt tctgttcctt tatcgtcggc tatgataacc atcaaagtgt ctccgacgtg
    17761 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
    17821 taacaaaaaa ttgcattcac tgcatgagat ttttttcatg ctttatttcc gaagaagctt
    17881 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
    17941 ttactgtttg attaaaaagg ttatacaagt tccacttgct taattactcg gctgttcttc
    18001 ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atgacgtagc tggctacact
    18061 tcctgtcata aagcgttcga ctgcatttag tccagactgc ccaaccatga ttagatcgat
    18121 tgcatatttt tttggcaatt tgactgccat ggattcacga acagagccgt atgcaataat
    18181 tccttctata tttgtgtaat caacagattt tccatattct ttacattctt cgatcatttg
    18241 cttggctgtt tcagtttgct gatcgactag attttgatca ataggagcat agcccataaa
    18301 attataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc
    18361 cacttctaac gcttttttga aagcagcaag tgcttgttca ctaccgtcaa tacctactaa
    18421 aatgttctta tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttattac
    18481 actttctagg aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc
    18541 taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc
    18601 ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt
    18661 ttcgatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa
    18721 gaaaaataca ctttttccag tttctacgta gcctgctaat tcttctaaac tatttgggat
    18781 aatcattctt tttcctctac tttctgttta tgtatttttc aattaaatta tacacctttc
    18841 tttcgaacta acaaacattg atcctctttt tataaaaaaa atctgacaaa aggttgcttt
    18901 tgccagattt tatagtttca ctatcgcgtt atttcccgta tacgatcgtg ttcactgttg
    18961 atttatctaa acctttcaat gtttgaatca acatttctcg tgcagcttca aaatcgcgaa
    19021 tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc actgtacttg
    19081 ggattccttc attttgtgta tgagcagcac ctgcatctgt accgcctttt gaaacaaagt
    19141 attgataagg gatgttgttt gtttcagcga tatctagcaa gtactcacgc aaacgaggaa
    19201 gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg
    19261 tgccattttt cgtcactgtg tcatctgctg cagagcagtc tactgcaaag aacagatctg
    19321 gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttct tggacattcg
    19381 cgcctgcaat caatgtatga cctaatgttt cattttgtaa cgcttctaaa gcttctaata
    19441 ccattgtaca gccatagcgg ttgtcccacg atttgctgat gatattcttc ccgttggctg
    19501 ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aagaacaccg taactcattg
    19561 cctcttctct tgattcaaac ccagcatcga acaaaatatc tgttacttct actgattttt
    19621 gtccgcttgt tcctcgtaat aaatgtgggg gaacggaaga agaaatacat ggataattct
    19681 taccagtaga ggttttaagt gtaaaacgtt gagcagaaac aacgtaaggg ttccatccgc
    19741 ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg aatccgactt
    19801 catccatatg tgctgcgacc attacacgag gtgcatccgc ttcttcatgg tggcgcagcc
    19861 caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc atatgacttt
    19921 ccatataggc gcgaacgtct tgttcaaaac cgcttgttcc ttgtaattct gttaactctt
    19981 tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag actaaactcc
    20041 tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc gatcgtattt
    20101 attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg agagtgtggt
    20161 aaaataaaca caaagagtag caagtgagaa gaaggaggta tatccacatg tatgaagaaa
    20221 gcgaattaag ctattttaaa ggcgggttag ccgttgggat cggtctagga ttattgagtg
    20281 gggtagcttc aacgatttgg tatcatcgca aaaaagtgtt atcagcagac caagtgcttg
    20341 aaaatatcaa aacatctttt ttaaaagaag gcgcaatcga aggtgcttgg atctcttttg
    20401 aaaaagaacc tattcggaaa ttcgccgttc ggtcgagtgc ttatcggggt gggctttccc
    20461 ggatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat acaggaaccg
    20521 tattagacat ttcaagaaaa aagggcgcaa tagattaata cgtcaaaaaa atgaaataac
    20581 gaatacgcac ccactatcta aaagagaggt atcctatcct tcttttatag tgggtgattt
    20641 ttataaaaat agggctattt cctcttggaa tagctagtat ttgtagttat tatacggtat
    20701 tcttgaaata agattagttt tattatataa ggagagtgac aaatggaatc acgtgaaaaa
    20761 attgccgtgc tcattccttg ttataacgaa gaggcgacaa tttcaactgt tattgaagat
    20821 tttaaaaggg aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga
    20881 acatacgaat tggccgttca aggcggagca atcgtaaaga aagaaccacg tcaaggaaaa
    20941 gggaatgtaa tccgtcaaat gttttttgat attgatgccg attattattt gatggtagat
    21001 ggtgatgata cttatcctgc agaagctgca catggtttgc ttgagaaatt acgttctggc
    21061 gaagcagata tggtcattgg cgatcgtcta tcaaatggaa cctattttga tgaaaataag
    21121 cgtcctttcc atgattttgg gaataatctg gttaaaaata caattactta cttatataaa
    21181 acaaaaattc gcgatgtcat gaccggctat cgaggattca atcgaatttt tgtcaaaagc
    21241 ttccctatta tgagttctgg ttttcaaatc gaaacagaat tgacgattca tgctttagat
    21301 aaaaaattca agttcgttga gattccaatc gattatcgcg atcgtcctga aggtagcgaa
    21361 tcaaaattga atacattctc tgacggattc aaagtgatca tgatgatcat caaaatgtgc
    21421 aaagattata aacctttgct tttctttggg atctggacca tcttcttctt tttatttgga
    21481 ttggtcatgg ggattcctgt gattcgtgaa tatatgctga caagctttat tacacgcatc
    21541 ccttctgcga ttttaagtac tggattgatg actttagccc ttctttcatt agtaacaggg
    21601 ttgattttgg atacggtcgt cacaaatgcc aagaaagaat atgaattgaa tttataccat
    21661 gtctaccacg aatatcaaaa taaagaaatg aagtaggagg ccttttttat gcataagaaa
    21721 aaggagaatc tatttcctac tttttttgca actttatttg cgtcaggttt tgcttttttt
    21781 acttcttatg gtctaggaac tttgaatcca acagtaatca ttggtttagc catggttttg
    21841 ctacttgctt ttaagcaata tggaatagag ttgttttcaa taaaaaacaa agttgcagct
    21901 attgcccgat tgtttttttc tgcagctttt gctcttacac tttctattgg cagacagata
    21961 caatttcagt atgtctcagc aaactataca aagacaacga ttgctgatgg aagtattttt
    22021 tactttcttg tttatttctc catttttgtg tattcttttt tatttttaca atcgatttat
    22081 ttatcaatgc caaaactgga aacatttttt actgctcaaa acacacaggc tttctcttca
    22141 agaaaatatt ttttattctt tactgtcttt ttagttctgt gttggcttcc ttatctgttg
    22201 gcattgtatc ctggagtggt tttgcctgat tcgttaagtt cggtcgccca atctatgggt
    22261 gatgttccta taagcaatca tcatccagtc ttattcacct tactggtcaa gctatttgtt
    22321 catgatttac caactgatac gatcaatcac ggtgtatttt tattttcgat ttttcaaaca
    22381 ttgataacag cttcagcgat aagttattct ctttgctggc tgttaaaaag aaacatccat
    22441 ttttttccgg tactcattag tttatgctat tttgcatttg caccggtttt tccaatctac
    22501 gccttgaaca tgcaaaaaga tactttgttc tctatcgctt gctttttatt ttccttagcg
    22561 gtttttgact ttatttttca acgagaaaag ccaacgattc tcaatcagat ttacgtaatc
    22621 atccttgctt tactgaccac ctatctaaga aataatggga tttatgtagt aaccggcaca
    22681 ggtttgattt gcggcttgat tattttgaaa caaaaaaggt atcaagcatt ctttcaagtg
    22741 gtgctgggtt atacagtgat tgctggacta ctcatccacc cgttgatggc tcgttatgct
    22801 attcctacag aaactgctga acaattaggt gttcctttgc agcaaatttc tagaactatt
    22861 gttatggatg gtgacttgtc taagaaagat aaagcattca tgaatcagct actcccgata
    22921 aaagattatg aagcgtattc tcctagtctt gcagactcaa tcaagtggca taaaaatttt
    22981 aacaatgagt ttttgaatac acataaaaaa gaatttctat cactatgggc aagaaattta
    23041 ccaagtaata taaaaatcta tacagatgct tatattttag aaacgttcgg gttctgggta
    23101 cctggtacaa aaaatagtta cggcttttta gatactcgtg taaacacgaa taattatggt
    23161 atgaagcaag tagatttgat tgaacatctt actggattgt caggaatgaa aaaaatcatc
    23221 gatgctcgtg atttctttgg ttccggcaca ttactgtggt tagctatcgg cagtgcactt
    23281 ttagcaatct ttaagaaaaa atatcggtat ttactccttt ttcttcccgg tatgcttgtt
    23341 tttgctacta ttatgattgc tactcctgtg gcttttagtt tacggtatgt gtttgtttta
    23401 gcattaggtc tacctgttta tatcctctct ccttttttac tagataaaag agaaaaaatg
    23461 aaaacagata taggagatac tcatgaaact atttaaaaag atattttttg cttttgttct
    23521 cttgatcgct agctattgcg tactactgac aattactttt ttcattccgc agcactctat
    23581 tgaatcgaat gcagaaaaat ctctacagat ggttgaaaaa gaaggaatgt atccttctat
    23641 caatcaagga aatatgctag gcactcggtt agataatttt actgatcatt tgatgatacg
    23701 aaaaacaaaa gcagatcctg aattgaactc cttggagaac gcgatgtcta tggcggatta
    23761 tccccgttat tggcatggtt atcagctttt tttacgacct ttactgctag ggatgagttt
    23821 ggggagtatt cgaatgattt acgcagccat tctttttctg ctgattggat tgacgagcta
    23881 ctttttaacc aagtgtagcg acatctattt aacgacagcc ttgttgattt cgcttgctat
    23941 aggaaatgct gcaacctttt tcttcagtat gcaattttca aatatttgga ttttgacttt
    24001 attgtctgta ttactatttt taacaaaacc acaactctcc aacaccaacc aacaattatt
    24061 cttgtatttc tttgtcattg gttctttagc taactttttt gacttgttga caacaccggt
    24121 catctcttgg gggatacctg tgatcattat ttattatgta acaaataagt atttaatgga
    24181 taaacgcagt tcattgagca aacaagtagg cagttttgtt tttaccggta tcttttgggg
    24241 attaggctat ggtttgacat gggtggctaa atggatccta tcatcgatca tattgaacaa
    24301 aaacattgta aaagatgcaa tcaaccaaat cctctttcga acggaaggaa acgacaaata
    24361 cccgttgcat cgattagaga tgctgaaaag caatattaga ttgatgtatc caaaagtagc
    24421 aatattattg ctattgatta cttgtcttgt ttttctctat tttgcttatc gtaaacgaaa
    24481 tagctggcgt ccgttccaac ttctagtttt gtttgtttta gctgctgtaa cgccgtatat
    24541 ttggtacaat attttagcta atcactcaca gattcattat tggtttacgt atcgtacgca
    24601 aatcatcaca agttttgcta tacttagtgc tttcgccttt ttgatccctc ctgagaaatc
    24661 aaaagacgaa ctaaattact tttaagaata ccgatgtact aattcatttt taaacataaa
    24721 aactccagct gtcagagata gccggagt
//
LOCUS       DAFCLG010000029        24288 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFCLG010000029 DAFCLG010000000
VERSION     DAFCLG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.029"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..805)
                     /locus_tag="LND56_001123"
     CDS             complement(<1..805)
                     /locus_tag="LND56_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6743060.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVK"
     gene            complement(1029..2969)
                     /gene="thrS"
                     /locus_tag="LND56_001124"
     CDS             complement(1029..2969)
                     /gene="thrS"
                     /locus_tag="LND56_001124"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK6743061.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3306..3485)
                     /locus_tag="LND56_001125"
     CDS             complement(3306..3485)
                     /locus_tag="LND56_001125"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBK6743062.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3532..5355)
                     /gene="cadA"
                     /locus_tag="LND56_001126"
     CDS             complement(3532..5355)
                     /gene="cadA"
                     /locus_tag="LND56_001126"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6743063.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5633..7912
                     /locus_tag="LND56_001127"
     CDS             5633..7912
                     /locus_tag="LND56_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6743064.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8005..8652)
                     /locus_tag="LND56_001128"
     CDS             complement(8005..8652)
                     /locus_tag="LND56_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743065.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8831..9550)
                     /locus_tag="LND56_001129"
     CDS             complement(8831..9550)
                     /locus_tag="LND56_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6743066.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9681..10832)
                     /locus_tag="LND56_001130"
     CDS             complement(9681..10832)
                     /locus_tag="LND56_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBK6743067.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11049..11789)
                     /locus_tag="LND56_001131"
     CDS             complement(11049..11789)
                     /locus_tag="LND56_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6743068.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11793..12116)
                     /gene="rsfS"
                     /locus_tag="LND56_001132"
     CDS             complement(11793..12116)
                     /gene="rsfS"
                     /locus_tag="LND56_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK6743069.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12173..12766)
                     /gene="yqeK"
                     /locus_tag="LND56_001133"
     CDS             complement(12173..12766)
                     /gene="yqeK"
                     /locus_tag="LND56_001133"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBK6743070.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12750..13400)
                     /locus_tag="LND56_001134"
     CDS             complement(12750..13400)
                     /locus_tag="LND56_001134"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK6743071.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13410..13721)
                     /gene="yhbY"
                     /locus_tag="LND56_001135"
     CDS             complement(13410..13721)
                     /gene="yhbY"
                     /locus_tag="LND56_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK6743072.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13737..14846)
                     /gene="yqeH"
                     /locus_tag="LND56_001136"
     CDS             complement(13737..14846)
                     /gene="yqeH"
                     /locus_tag="LND56_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBK6743073.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14843..15370)
                     /locus_tag="LND56_001137"
     CDS             complement(14843..15370)
                     /locus_tag="LND56_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBK6743074.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15550..16335)
                     /locus_tag="LND56_001138"
     CDS             complement(15550..16335)
                     /locus_tag="LND56_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK6743075.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16328..17197)
                     /locus_tag="LND56_001139"
     CDS             complement(16328..17197)
                     /locus_tag="LND56_001139"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBK6743076.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17198..18577)
                     /locus_tag="LND56_001140"
     CDS             complement(17198..18577)
                     /locus_tag="LND56_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK6743077.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAKQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18585..19004)
                     /gene="fabZ"
                     /locus_tag="LND56_001141"
     CDS             complement(18585..19004)
                     /gene="fabZ"
                     /locus_tag="LND56_001141"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6743078.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19029..19505)
                     /gene="accB"
                     /locus_tag="LND56_001142"
     CDS             complement(19029..19505)
                     /gene="accB"
                     /locus_tag="LND56_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK6743079.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19508..20743)
                     /gene="fabF"
                     /locus_tag="LND56_001143"
     CDS             complement(19508..20743)
                     /gene="fabF"
                     /locus_tag="LND56_001143"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK6743080.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20762..21499)
                     /gene="fabG"
                     /locus_tag="LND56_001144"
     CDS             complement(20762..21499)
                     /gene="fabG"
                     /locus_tag="LND56_001144"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBK6743081.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21511..22428)
                     /gene="fabD"
                     /locus_tag="LND56_001145"
     CDS             complement(21511..22428)
                     /gene="fabD"
                     /locus_tag="LND56_001145"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6743082.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22535..22759)
                     /locus_tag="LND56_001146"
     CDS             complement(22535..22759)
                     /locus_tag="LND56_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6743083.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22812..23774)
                     /locus_tag="LND56_001147"
     CDS             complement(22812..23774)
                     /locus_tag="LND56_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBK6743084.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23784..24224)
                     /locus_tag="LND56_001148"
     CDS             complement(23784..24224)
                     /locus_tag="LND56_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6743085.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6640 a         5418 c         4234 g         7996 t
ORIGIN      
        1 ttttcacttt ttcgctaagc ttttcgtttt tagccatata gaaataaaaa atgatatcga
       61 tcgtagcaaa ttgggccaaa agtgaattag ttgccgcact tcgattcgtg atttcttttg
      121 gtctagatgt ttgtacgagt acatcggcta tcttgctaag cgggttattt ccaaattgcg
      181 tcaacgcaat gatttcgata ttcatgcttt tcgccagctc ggctaatgcg actacatctg
      241 cacttctacc tgagtttgaa acaagccaaa gcactttctt tttctcattg ctgacaagtt
      301 gcgggagcaa gacgtggata tctttttcaa aaatgattgg cttaccgaca cgagaccatt
      361 tttgcagaat atcttctact actaaagaag aggcgccaac cccatataaa aaaacagtct
      421 ctgccgattc caaacactct acgacttttt ggatcgtttc ttcttctaga atatctactg
      481 tttcttttat cgctacttgt gcattgttac caagtttgtt tttgatagag taaaaggact
      541 catttgcttc tatatcacta aaaccttcat cgattccagt ctgtagttct gttgaaatcg
      601 tgattttgaa gtccgtcaga ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg
      661 gcgcactcaa cccggaagct tctgctaatt cattagctgt cattgtaggg accttctcaa
      721 tattaccgat aatgtagttg gccaactttt tttctgctga agaataatct tccatcatac
      781 tttcaatacg actaattacg ctcataagtt cctccaccct tttaatgata gtctatgtaa
      841 gtataccata tcagaccctt attcacggtc gtatactgga taaaaaacaa aaaaacaatc
      901 cgcccaacag aatatcttat cccatcaatc ccacagattc tattgagcat ctgtgtcttt
      961 gaggaatacc tgttatctat tgttcggatt gctttctatc tactactttt ttacaggaaa
     1021 tttacctatt agttttctct gctgtaattt ttgacttctg cttccatagc atcaataaag
     1081 atattcaaat cttctacagc tgtttcttta cttccataac gacgtacgtt cactgttgca
     1141 tcttccaatt ccttgtctcc aacaactaat tggtaaggga tcttttgtgt ttgagaggca
     1201 cgtatcttat agcccatttt ttcattacga tcatcaactt ctacacgcaa ccccttcatt
     1261 tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg catcaacaga aacagggatg
     1321 attgttgctt gtataggtgc caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa
     1381 taagcgacaa aacgttccat tgtagaaaca attccgcggt ggataactac tggacgatgc
     1441 gtattttccc cgtcttctcc aacatatgtc aaatcaaagc gctctggcaa taagaagtct
     1501 aattgtatag ttgataatgt ttcttctgta ccaagagctg ttttgacttg aacatctagt
     1561 tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt aatctagttc taattcatcc
     1621 atagcggctt tcagcattgc ttgggctttt tcccacattg cgtcatcatc aaaatattta
     1681 tctgtattgt ttggatcgcg atagctcaag cggaaacgat aatctgtaat attgaagtca
     1741 gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt catctttgat ttgatcagga
     1801 cgaacaaatg tatgaccatc attcaacgtc atttctcgta cacgttgtaa acctgacaaa
     1861 gcacctgatt tttcatagcg atgcatcatt cctaattcag cgatacgaat tggtaattca
     1921 cgataagaat ggatcgtgtt tttgtagacc atcatgtgat gagggcagtt cattgggcga
     1981 agaacaagca tttcgccatc tcccatatcc attggcggga acatgtcttc ttgataatga
     2041 tcccagtgtc cagatgtttt gtacaactca acatctccca ttattggtgt atatacatgt
     2101 tggtaaccta gactaacttc tttgtctacg atataacgtt cgatcgtgcg tcggattgtt
     2161 gccccttttg gaagccagaa tggcaaacca gaacctactt ctggggaaac catgaacaag
     2221 tcaagttctt ttcctagttt acgatggtct ctttcttttg cttcttcacg gagacggata
     2281 tattctttca aagctttctt gtcaaaaaag gctgtcccgt atacacgttg catcatttga
     2341 ttgtttgagt ttccacgcca ataagcacct gccactgata ataatttaaa tacttgtata
     2401 cggccagtgg atggaacatg tggtccacga cacaaatcga tgaattcgcc ttgttgataa
     2461 gcagtaatca cttcttcttc tggcaattca ctaatcagtt ctactttgta cggatcactt
     2521 gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc gttcgatcgg cagattttct
     2581 ttgacgattt tcatcatttc tgcttcgatt gccggcaaat cttctgcagt tacttggttt
     2641 tgtccattat ctgtatcgta gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg
     2701 atatttggat agaggcgacg catggcttgg gccatcaaat gagctgctga atgacggact
     2761 aagccaagtg catcttcatg gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt
     2821 ggacgagtca aatcaacgag ttttccgttc actttaccag ctaaagcttt ttttgctaag
     2881 ctttttgaaa tgctttcagc aattgccaat gttgttgttc cagactcaaa ttctttgact
     2941 gcaccatctg gaaaagtaat ttttatcata atagtttcct ccttgaaatg ttttgattga
     3001 tcagatcatc aagctgaaaa acaaaaaaat cctagcatct cttgaaaaga gatactagga
     3061 cgaaaagttc gtggttccac ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac
     3121 ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg tcatcctttg gttttctcct
     3181 ggaaaagttt cagcctagct tttcctctct tatcgaagac tctcaaattc agttggcttt
     3241 ctcatcaatc aattatgtgt ttatttaacc actaataaat agaaaaatca agtgggaaac
     3301 taaacttatt ttttccattc cccatttaca ccgtatgttc cttttttcat ctcattgatg
     3361 atgacatgga tgtgttcttt tggtgcctgc gtgtttttat gaacagcttc cgtgatatct
     3421 ttcatcatat tttctaattg ttccggactg cgaccttcta ctaattcgac atgtacgaat
     3481 ggcataatgc tctctcctct ttctgatcgt tcttttttca actcgattct atcatactat
     3541 gtttgtcttg ggtactgaat caggaaaaat ggctggatct ttttttgctc tcaaggcact
     3601 caaaatagag accgtatcca cgacttcttg caaaacagct ccgaataatg cggggattag
     3661 tcccgtactt gcaattaaca tcaaaaaaat acaaataaag atgccaatca gtacagactg
     3721 tttcgctatt ctcatcgtat ctcttgaaat cactactgct tttgctacac gcaagagatc
     3781 atctttcaag atgaccgcat ctgcactttc actagcagct gtagaaccat gagcccccat
     3841 tgcaataccg atatctgcag cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat
     3901 cgcaacaggt ctttgatctt tagggatact ttctaaaatg gtcagtttat cttgaggcaa
     3961 gctacgggca tgtacagaat ctaattggac tttttctgca atcgtttgag caatggcaga
     4021 tccatccccg gtgatcatca tcaaattttt cacatcaagt tttcttagct ccttgattgt
     4081 ctttgctgct tctgggcgaa tctgatccga aaataggatc gttcctgcaa aagatcgatc
     4141 aattgttaca tagattgctg tattggacgt ttctatttct tcagctgaaa caaaagaggc
     4201 acgtccaatc tttacttcat gattattgac tactgcttgg attccttgac cggtcacttc
     4261 tttcagttga ctaacaggat acagttggct gtttttcacg ttgttcacta acgaacgagc
     4321 taagatatgg ctagattcct gttctgcact agctgcatac agaagtagct cttctttttt
     4381 tattccattg actggctgaa tcgcctctac ttccaatatc cctttggtaa gcgttcctgt
     4441 tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca atcgttgttc cagtttttac
     4501 aacgatcccg tttttacttg aacggctcat tcctgcaact aaagcaatgg gcgcagctag
     4561 aatcagtgga caaggtgatg ccactaccag tacctcagca aatcgtaaag ggtcttttga
     4621 gaaccaccaa gaaataccac cgatcaaata agcaatcaac gtgaaaggga ctgcataacg
     4681 atcagctaaa cggacaaatc gggcaggctt ttcttttgat tcttcgacta gtttaataat
     4741 catttgatac tggctgtctg cggcacgttt ttcagcgatc atttggatcg atccgtctcc
     4801 atttaaagac cctgacataa tctgatctcc aggtttcttg gtttccgggc gggactcacc
     4861 tgtcaaagaa gattcatcga ccgaagattc tcctttggtc actcgaccat ctgcgggtac
     4921 aagttcgcca ggcttgacta gtaactggtc atcaatattt atttcatcga ttactatatc
     4981 ctgcaatttt ccttctttga atcgatgggc aattcttggg gtgttatcta acaaagactg
     5041 cagttcttta cccgctcgtt tattcgcgta atcctctaga ctatctccgc ccgtaagcat
     5101 gatcaatatc atcaaactag cccagtattc tttgacggct aatgttgcaa ggattgcggt
     5161 aatcgctaat acatctacgc catatcttcc tgaacgtaac gtcttgatca tttccaaaaa
     5221 catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg
     5281 gtgaaaaaca aattcactta ataaagctat cccaccaata agcaaagtaa gtaaaaattt
     5341 tttgaaatgt gtcattgttt tttctcctct tttatgtgac ttagagaggt tagtaataaa
     5401 tttccagctg cctctttcat cttaagcata gtccttgact agcacttttt aaaacaatag
     5461 gccttaattg aaaacaacta tcactatcag cgtttccaaa attcatcagt tctacaaaca
     5521 aaaatattca gcaaggatta ctttttcgat tcatcagtta aaaagtacct tttttcgaaa
     5581 aactatgcta tacttttaat tcgttattta cggcggaagg agcaaacatg cgatgaatga
     5641 gaaacaacta gaacaaacgc acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa
     5701 tatgctggat acaaaacaac aagaattgac tcaggaaatg aatcattcga caaaagacac
     5761 agctaatcac aagattcgag ctggttccaa tgaagctttt tatgaatctg ctgttgaata
     5821 tcgccaacat gagcaggaat tgcttttgaa ataccatact cttgaagcac agcaaaaacg
     5881 cctgcaaacg ttgtctgtca tgaaagatag tccttatttt gcccgtatcg actttaaaga
     5941 agaccgtgaa acagaaacat tatatctagg tatcgcttct ttaagagatc tggcagaaga
     6001 aacaatcgtt attgactggc gcgcaccaat tgccaatctt tattatgaag gtgagattgg
     6061 tcccgcattt tatgaaactg aaacagatag aatagatgtc gatctgcagt tgaaacgaca
     6121 atttaaaatc caagaaggac gtattttatc tatggtagat acgtcggaag taatcaatga
     6181 tgactttttg ctagagatat tggatgaatc atcaagcaac catatgaaaa acatcgtctc
     6241 tacgatacaa aaagcacaaa atgcaatcat tcgggacaca acaagtaaag taatgctgat
     6301 tgaaggaatc gccggaagtg gaaagacatc cgctttgctt caacgtattg cttttctgtt
     6361 gtatcataat cgcaagtggc tcgaggctga aaacgttctt ctattttcac ccaaccattt
     6421 gttttctgat tacatctcga ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca
     6481 gacattcaaa aattatttgg tgcagctgct gccagagttt tctttactag atgaacagca
     6541 acaagaaagt ggttttttgc taggggaaaa agatccgata caagtgatga aaagcggatt
     6601 gaccttagtc gatcagattg atcgctatat ccaaggcatc acaaactacg gtcccttgtt
     6661 ccgtgatatg aaaataaatg gccgtacgat cctatcaaag gaatcgattc gccaatggta
     6721 taaagaaaca aatgaacaat tacctttaca tcatagactt tctctattac aaacgaagct
     6781 cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct
     6841 ggcggaagaa cagctccaag aactatacgc aacagatcca aatcttgaat atacagaaca
     6901 aaaggagcgt tcattacgta aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg
     6961 aatccatcga gggatcatga attatcaatt tgtcaatata acgaaacaat atctccactt
     7021 tctacaaact gtgccacttt ctcttctaac acgacataaa atcagcgaga ccaggtggca
     7081 ggaacatttg acaactgttc gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc
     7141 tgtactattc tttttactga tgcgccgaac atatccagtg acggttacac acaaagctcg
     7201 ctatatcttt attgatgaaa tgcaggattt cgcaccatca caagtggcat tgctgcaagc
     7261 cttgttccca aaagcaaatt tgactttctg tggagatcta aatcaaaatg tctttggaaa
     7321 tgaaacgatc gttggatcat tggaagacct tttcgtggac cgtgaagtca ctcgtttcca
     7381 attaacgaca agctatcgct ccacaaaaga aatcactgat tttgctaatc attttattgt
     7441 gaaagaacat caagtagaaa caacagcaag aaaaggagac cttcctttgc tcatccaagg
     7501 aacaacagat gatatcaaat tgaattggtt agcagatacc attcgaacga ctcagggaaa
     7561 agcccggtat tggcgtacag caatcatagc taaaacacaa gaagaatgcg aagctcttta
     7621 tgctcaactg ccagacgagt tgaaacaaca agtccagctg attttaaatg agagtgactt
     7681 tatgaagcgt tccattattt tacttccagc ctttttagca aaaggtctgg agtttgaccg
     7741 agttttcttg tgggatgtag atgcagaaca tttttcttct cagcaagaca aattgatcct
     7801 ttacaccatg tgtacacgtg cgatgcatga attgaccctt tttacctcca ataatgaaag
     7861 tcctttgtta aacaatatgg atatttcatc ttatgagaaa aaagtactgt aagtaaaaag
     7921 acaactgcta tcattaatgt tagcagttgt ctttttattt acaataataa ttgcagaaaa
     7981 agtaattcaa tcatttaagg tcatttattc tatatataaa tccaaatctg tttcaggata
     8041 ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg gatattctgg
     8101 gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt aaattcgatt
     8161 ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc cacaatccaa
     8221 aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt ttttcgtgtc
     8281 tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc tccactctaa
     8341 tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga
     8401 acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg gtgcgcggtc
     8461 caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc ctaatcgata
     8521 gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag ttacgttcac
     8581 agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat
     8641 ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt tcacatgata
     8701 cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac acaaaaaagc
     8761 aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa
     8821 aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat
     8881 cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct
     8941 cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta
     9001 atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt
     9061 cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag
     9121 cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg
     9181 aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat
     9241 tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat
     9301 agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa
     9361 taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt
     9421 ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg
     9481 cataaatccg gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt
     9541 tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct
     9601 gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct
     9661 tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga
     9721 atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc
     9781 tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt
     9841 ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct
     9901 tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat
     9961 ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt
    10021 ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta
    10081 gaagataaca accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa
    10141 gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct
    10201 aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga
    10261 tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt
    10321 tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg
    10381 ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac
    10441 agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg
    10501 ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt
    10561 tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg
    10621 ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta
    10681 tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc
    10741 tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga
    10801 ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa
    10861 aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa
    10921 actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta
    10981 tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg
    11041 caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct
    11101 tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt
    11161 aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga
    11221 ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg
    11281 cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg atagctatat
    11341 tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt
    11401 tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata
    11461 tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg
    11521 agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca
    11581 atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag
    11641 gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt
    11701 aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg
    11761 taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact aagatcgact
    11821 agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa
    11881 acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca
    11941 atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg
    12001 ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca
    12061 tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca
    12121 atttttaatt gttctttact atctatgatg atttcctcct aatatgagca gcttattttt
    12181 ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa
    12241 gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg
    12301 caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta
    12361 cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg
    12421 catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa
    12481 gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga
    12541 ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa
    12601 tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta
    12661 ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt
    12721 tttctctaga ataagcagta tattcattac tatagtccat attcatacaa cccttcattt
    12781 tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc
    12841 ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga
    12901 tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc
    12961 agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag
    13021 taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt
    13081 ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct
    13141 aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt
    13201 ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca
    13261 gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc
    13321 tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc
    13381 ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt
    13441 tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg
    13501 atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta
    13561 ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg
    13621 atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct
    13681 ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa
    13741 atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact
    13801 ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca
    13861 gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt
    13921 aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga
    13981 atatttacat catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca
    14041 aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt
    14101 ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt
    14161 tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc
    14221 tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc
    14281 gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg
    14341 atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca
    14401 tgagatttct tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca
    14461 cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact
    14521 ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt
    14581 gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat
    14641 tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttggat ctcattataa
    14701 tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa
    14761 gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag
    14821 ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt
    14881 ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac
    14941 tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac
    15001 gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt
    15061 gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa
    15121 gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa
    15181 cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg
    15241 gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat
    15301 tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat
    15361 ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa
    15421 ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga
    15481 aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac
    15541 aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc
    15601 taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt
    15661 atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa
    15721 ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc
    15781 gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc
    15841 cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc
    15901 aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat
    15961 agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat
    16021 caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg
    16081 atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc
    16141 tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat
    16201 cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc
    16261 ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg
    16321 tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga
    16381 ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga
    16441 attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc
    16501 ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa
    16561 aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt
    16621 cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc
    16681 cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg
    16741 ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga
    16801 taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag
    16861 tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt
    16921 cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt
    16981 catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag
    17041 ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact
    17101 ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat
    17161 tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt
    17221 ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta
    17281 tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg
    17341 tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat
    17401 ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa
    17461 ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc
    17521 ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt
    17581 gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca
    17641 cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga
    17701 ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg
    17761 ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt
    17821 ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc
    17881 gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga
    17941 tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc
    18001 caaatgccgc tgctgcttct tgcttagctg aagaaaaatg ctgtgggagc tcttcttttg
    18061 attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat
    18121 aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc
    18181 ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca
    18241 ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg
    18301 aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg
    18361 tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg
    18421 ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg
    18481 aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga
    18541 tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag
    18601 gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg
    18661 gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact
    18721 ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt
    18781 tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct
    18841 ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt
    18901 ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt
    18961 ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc
    19021 tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg
    19081 ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt
    19141 ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg
    19201 aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc
    19261 tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct
    19321 tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata
    19381 ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa
    19441 tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata
    19501 tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta
    19561 atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca
    19621 agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta
    19681 atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca
    19741 tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg
    19801 cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga
    19861 cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag
    19921 aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga
    19981 tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa
    20041 cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac
    20101 gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct
    20161 cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt
    20221 tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac
    20281 aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca
    20341 ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt
    20401 tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat
    20461 tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat
    20521 agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga
    20581 aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg
    20641 gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa
    20701 taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt
    20761 cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat
    20821 tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg
    20881 gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg
    20941 tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca
    21001 cagattttgt taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat
    21061 ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca
    21121 tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt
    21181 ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt
    21241 ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt
    21301 tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt
    21361 cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt
    21421 ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat
    21481 tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt
    21541 ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct
    21601 taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg
    21661 gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt
    21721 ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa
    21781 ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg
    21841 aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa
    21901 aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata
    21961 atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc
    22021 aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat
    22081 ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc
    22141 tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg
    22201 ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg
    22261 cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat
    22321 ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat
    22381 accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa
    22441 tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca
    22501 ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag
    22561 tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat
    22621 cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga
    22681 tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt
    22741 gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat
    22801 aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct
    22861 gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc
    22921 aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac
    22981 ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga
    23041 tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg
    23101 tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct
    23161 gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt
    23221 ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag
    23281 agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt
    23341 agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt
    23401 ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg
    23461 atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa
    23521 tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt
    23581 tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat
    23641 cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg
    23701 gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt
    23761 ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc
    23821 agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc
    23881 atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc
    23941 aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact
    24001 aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct
    24061 gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac
    24121 agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag
    24181 acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtccacta
    24241 attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactt
//
LOCUS       DAFCLG010000030        24271 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFCLG010000030 DAFCLG010000000
VERSION     DAFCLG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.030"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..572
                     /locus_tag="LND56_001149"
     CDS             <1..572
                     /locus_tag="LND56_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6743086.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="LND56_001150"
     CDS             576..1217
                     /locus_tag="LND56_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743087.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="LND56_001151"
     CDS             1214..2131
                     /locus_tag="LND56_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6743088.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="LND56_001152"
     CDS             2128..2799
                     /locus_tag="LND56_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743089.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="LND56_001153"
     CDS             complement(3340..3828)
                     /locus_tag="LND56_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBK6743090.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="LND56_001154"
     CDS             complement(4029..4295)
                     /locus_tag="LND56_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6743091.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="LND56_001155"
     CDS             complement(4295..4558)
                     /locus_tag="LND56_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6743092.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="LND56_001156"
     CDS             5034..5219
                     /locus_tag="LND56_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6743093.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="LND56_001157"
     CDS             complement(5361..7184)
                     /locus_tag="LND56_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBK6743094.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="LND56_001158"
     CDS             complement(7782..10487)
                     /locus_tag="LND56_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK6743095.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="LND56_001159"
     CDS             complement(10793..11740)
                     /locus_tag="LND56_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBK6743096.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="LND56_001160"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="LND56_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK6743097.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="LND56_001161"
     CDS             complement(12375..12737)
                     /locus_tag="LND56_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6743098.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="LND56_001162"
     CDS             complement(12748..13869)
                     /locus_tag="LND56_001162"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK6743099.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="LND56_001163"
     CDS             complement(13885..14235)
                     /locus_tag="LND56_001163"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK6743100.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="LND56_001164"
     CDS             complement(14365..15876)
                     /locus_tag="LND56_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6743101.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="LND56_001165"
     CDS             complement(16198..17550)
                     /locus_tag="LND56_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK6743102.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="LND56_001166"
     CDS             complement(17703..18779)
                     /locus_tag="LND56_001166"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK6743103.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="LND56_001167"
     CDS             18975..20720
                     /locus_tag="LND56_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBK6743104.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="LND56_001168"
     CDS             complement(21000..21437)
                     /locus_tag="LND56_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK6743105.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="LND56_001169"
     CDS             complement(21421..22794)
                     /locus_tag="LND56_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743106.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="LND56_001170"
     CDS             complement(23116..23604)
                     /locus_tag="LND56_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6743107.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="LND56_001171"
     CDS             complement(23626..24228)
                     /locus_tag="LND56_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6743108.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5163 c         4486 g         7662 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta tagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcat aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAFCLG010000031        23829 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFCLG010000031 DAFCLG010000000
VERSION     DAFCLG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.031"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(53..787)
                     /locus_tag="LND56_001172"
     CDS             complement(53..787)
                     /locus_tag="LND56_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6743109.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="LND56_001173"
     CDS             1114..1524
                     /locus_tag="LND56_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK6743110.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="LND56_001174"
     CDS             1517..2218
                     /locus_tag="LND56_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK6743111.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="LND56_001175"
     CDS             2218..3804
                     /locus_tag="LND56_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743112.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="LND56_001176"
     CDS             3823..4044
                     /locus_tag="LND56_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBK6743113.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="LND56_001177"
     CDS             4041..4802
                     /locus_tag="LND56_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBK6743114.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="LND56_001178"
     CDS             5182..5691
                     /locus_tag="LND56_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6743115.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="LND56_001179"
     CDS             complement(5766..6305)
                     /locus_tag="LND56_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBK6743116.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LND56_001180"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="LND56_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK6743117.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="LND56_001181"
     CDS             7318..8169
                     /locus_tag="LND56_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK6743118.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="LND56_001182"
     CDS             8197..8832
                     /locus_tag="LND56_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBK6743119.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="LND56_001183"
     CDS             8852..9625
                     /locus_tag="LND56_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6743120.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="LND56_001184"
     CDS             10033..10830
                     /locus_tag="LND56_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBK6743121.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="LND56_001185"
     CDS             10808..11221
                     /locus_tag="LND56_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6743122.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="LND56_001186"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="LND56_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6743123.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="LND56_001187"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="LND56_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK6743124.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="LND56_001188"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="LND56_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBK6743125.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LND56_001189"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="LND56_001189"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBK6743126.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="LND56_001190"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="LND56_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBK6743127.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="LND56_001191"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="LND56_001191"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK6743128.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="LND56_001192"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="LND56_001192"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK6743129.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="LND56_001193"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="LND56_001193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK6743130.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="LND56_001194"
     CDS             21905..22483
                     /locus_tag="LND56_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6743131.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="LND56_001195"
     CDS             22535..23017
                     /locus_tag="LND56_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBK6743132.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="LND56_001196"
     CDS             23134..23508
                     /locus_tag="LND56_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743133.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atagaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DAFCLG010000032        23654 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFCLG010000032 DAFCLG010000000
VERSION     DAFCLG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.032"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..1526
                     /locus_tag="LND56_001197"
     CDS             99..1526
                     /locus_tag="LND56_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743134.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1618..1983)
                     /locus_tag="LND56_001198"
     CDS             complement(1618..1983)
                     /locus_tag="LND56_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6743135.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1980..2345)
                     /locus_tag="LND56_001199"
     CDS             complement(1980..2345)
                     /locus_tag="LND56_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6743136.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2537..2785)
                     /locus_tag="LND56_001200"
     CDS             complement(2537..2785)
                     /locus_tag="LND56_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743137.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2784..2912
                     /locus_tag="LND56_001201"
     CDS             2784..2912
                     /locus_tag="LND56_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBK6743138.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2969..4663
                     /locus_tag="LND56_001202"
     CDS             2969..4663
                     /locus_tag="LND56_001202"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBK6743139.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4667..4786
                     /locus_tag="LND56_001203"
                     /pseudo
     CDS             4667..4786
                     /locus_tag="LND56_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5437..6570
                     /locus_tag="LND56_001204"
     CDS             5437..6570
                     /locus_tag="LND56_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK6743140.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6601..6921
                     /locus_tag="LND56_001205"
     CDS             6601..6921
                     /locus_tag="LND56_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBK6743141.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6950..7414)
                     /locus_tag="LND56_001206"
     CDS             complement(6950..7414)
                     /locus_tag="LND56_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6743142.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7545..8798)
                     /locus_tag="LND56_001207"
     CDS             complement(7545..8798)
                     /locus_tag="LND56_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6743143.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8827..9144)
                     /locus_tag="LND56_001208"
     CDS             complement(8827..9144)
                     /locus_tag="LND56_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6743144.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9170..9490)
                     /locus_tag="LND56_001209"
     CDS             complement(9170..9490)
                     /locus_tag="LND56_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6743145.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9954..11369)
                     /locus_tag="LND56_001210"
     CDS             complement(9954..11369)
                     /locus_tag="LND56_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6743146.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11386..12702)
                     /locus_tag="LND56_001211"
     CDS             complement(11386..12702)
                     /locus_tag="LND56_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6743147.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12852..13550)
                     /locus_tag="LND56_001212"
     CDS             complement(12852..13550)
                     /locus_tag="LND56_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK6743148.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13839..14799)
                     /locus_tag="LND56_001213"
                     /pseudo
     CDS             complement(13839..14799)
                     /locus_tag="LND56_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14913..15746)
                     /gene="msrA"
                     /locus_tag="LND56_001214"
     CDS             complement(14913..15746)
                     /gene="msrA"
                     /locus_tag="LND56_001214"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6743149.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15980..16723)
                     /gene="truA"
                     /locus_tag="LND56_001215"
     CDS             complement(15980..16723)
                     /gene="truA"
                     /locus_tag="LND56_001215"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6743150.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16914..17549)
                     /locus_tag="LND56_001216"
     CDS             complement(16914..17549)
                     /locus_tag="LND56_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK6743151.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17696..18937)
                     /gene="sstT"
                     /locus_tag="LND56_001217"
     CDS             complement(17696..18937)
                     /gene="sstT"
                     /locus_tag="LND56_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK6743152.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(19258..19935)
                     /locus_tag="LND56_001218"
     CDS             complement(19258..19935)
                     /locus_tag="LND56_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK6743153.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20186..20728)
                     /locus_tag="LND56_001219"
     CDS             complement(20186..20728)
                     /locus_tag="LND56_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6743154.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20733..20960)
                     /locus_tag="LND56_001220"
     CDS             complement(20733..20960)
                     /locus_tag="LND56_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6743155.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(21039..22919)
                     /locus_tag="LND56_001221"
     CDS             complement(21039..22919)
                     /locus_tag="LND56_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6743156.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23205..23540
                     /locus_tag="LND56_001222"
     CDS             23205..23540
                     /locus_tag="LND56_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6743157.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT         7342 a         4846 c         3992 g         7474 t
ORIGIN      
        1 ttatatgctt tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc
       61 ccatattgtg caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga
      121 aaaatatttt aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc
      181 tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata
      241 acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca
      301 ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt
      361 cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat
      421 taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag
      481 cttattttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg
      541 aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc
      601 tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg
      661 gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc
      721 tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac
      781 gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata
      841 aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt
      901 cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc
      961 ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa
     1021 accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat
     1081 tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag
     1141 tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta
     1201 attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc
     1261 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
     1321 aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata
     1381 taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac
     1441 cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta
     1501 tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta
     1561 tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca
     1621 cttattttgg acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg
     1681 gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc
     1741 gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt
     1801 tttaatagca ttgttctcgc tgactcggtc gctttgcact tttgtatgga caaataaata
     1861 tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat
     1921 atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt
     1981 catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa
     2041 gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc
     2101 ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa
     2161 cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg
     2221 acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg
     2281 atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga
     2341 aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt
     2401 taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt
     2461 ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag
     2521 caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt
     2581 tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac
     2641 ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt
     2701 tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa
     2761 gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag
     2821 gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca
     2881 gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac
     2941 atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc
     3001 cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga
     3061 tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac
     3121 tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc
     3181 tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg
     3241 aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc
     3301 aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg
     3361 cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc
     3421 tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc
     3481 acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg
     3541 tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat
     3601 cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc
     3661 tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga
     3721 ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac
     3781 caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt
     3841 cactaatggg tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga
     3901 aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa
     3961 cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac
     4021 cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc
     4081 aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga
     4141 tgtcaatatg tctatcgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga
     4201 aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca
     4261 gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt
     4321 agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa
     4381 taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga
     4441 tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga
     4501 gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc
     4561 tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga
     4621 tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa
     4681 agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat
     4741 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttgtga
     4801 aaaagctgcc ctgcatcaag taataagaac acccaagcta aaactgcttc ttatgttttt
     4861 gacttggttg gacttattac cgcagatgca agcttttgaa caccatttac caagaggtta
     4921 tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt
     4981 tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
     5041 ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac
     5101 gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt
     5161 tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat
     5221 ccaacatcat ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa
     5281 acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc
     5341 gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat
     5401 gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga
     5461 aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca
     5521 atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt
     5581 gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa
     5641 gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt
     5701 agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat
     5761 ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt
     5821 ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat
     5881 aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg
     5941 agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca
     6001 gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta
     6061 gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc
     6121 ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat
     6181 gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat
     6241 ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa
     6301 aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg
     6361 caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta
     6421 atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact
     6481 cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat
     6541 aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt
     6601 atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa
     6661 atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct
     6721 gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc
     6781 cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct
     6841 attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca
     6901 agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg
     6961 tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg
     7021 tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc
     7081 caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac
     7141 gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga
     7201 agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat
     7261 accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa
     7321 cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt
     7381 gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa
     7441 cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa
     7501 aaaaaaaacg gaaaacaatc acttgttctc cgttttttga ttaatcaagc atctaaagct
     7561 tcttttcttc tttcttggat cgtagctacc ttgacgaacg gtagatagat caaaatagaa
     7621 agaaccaaat tgacagcggc tagtacacca cctgcaatac tttttgttgc caatacaccg
     7681 ccaataattg gtggagttac ccatggaggc ataacggtag ctgctggcac taaacctgta
     7741 gaagtcgcaa agtaagcaac actaactaag accattggtg tcaaaataaa tggaatgaac
     7801 atgatcgggt tcaacacgat aggtaaccca aacatcgttg gttcattaat attgaagaca
     7861 cctggcgcaa tcgacaagtt tgttacaacc atatatggtt tgtttttacg tcctactaaa
     7921 taaatagcta acagtaaacc taaagttgca cctgttcctc ctaagttgac aaagctgtca
     7981 aagaaaggtt tgttcacgat aaacggtgcg ctatgcccag ctgcgattgc attaacgttt
     8041 gcttcaatgg ctggcgcatt gattgtttgc atcaaaggat cgaccatatt cgctccgtgt
     8101 aaaccaaaga accataagaa tggtgtgata aatgcaagca gtaaggcaga tggataagag
     8161 tttgcaagtc ccataaatgg ttcttggacc actgtataga aagaacctac gatatcatca
     8221 acaccgaatg cagcaaaaat tgcagcaact aaagcaaacg cagagatagt gatcattgct
     8281 ggtagtaatg aagcaaatgc ttttgcaact gccggtggca caccatcagg cattttgatg
     8341 atcaattttt catttccgct taaacgtaca aagatttctg ttgaaataat cccaacaata
     8401 atagcaataa acaaaccgtt tgagctcata cctaatgctc cgcctaaagc gaagaaagag
     8461 cctaaagaaa tcacacctgc ggaaacaccg tcttttcggt agccgttagc taaatgataa
     8521 gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg taccgttcca agcagcagcc
     8581 ccgaatcctt tccatgattc accaccaaaa attgagttca tcaattcttg gaacccggga
     8641 atcggtaagt tattgatcat tactgctaat gctcctaaga tcattaacgg catactcacc
     8701 ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac caatttttga tgctacaggt
     8761 ataaaatgtt tctccataaa tgcaacaaat ttatccataa atcttccctc ttttctttac
     8821 tgtagattac gcgttcaatc gacgatacag atcaatgatc tcttttgcta aatctgtaaa
     8881 agctatagat gtcatcagat gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg
     8941 atttccttgt gcttcttgtg tcagcatctc tgtttgagag tgatgtgctt ggactaaaga
     9001 ttcctctgca tctttgattt tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc
     9061 aatcgcttcc attgcatcgc tttttgcatt tccgccaaac atgatcagac ccatgatggc
     9121 ttctagattt ttttgatctt ccatttattg tatcctccga taatttttat tatttgttta
     9181 ttagtttttc tgcttggtcg agtacttttt caccattcat catgccatag tcagccatat
     9241 tgattacatc taaaggaatg cctttaggtg ctagcttttc agcaaattga ttttgcataa
     9301 agcgtacttg cggtcctaaa agtaaaacgt ctacattttt agattctagt tgattatccg
     9361 catctgaagc agatacagca aagatgtctg cttccattcc ttttgcttct gctgcctttt
     9421 gcatttttgt gactaggaga cttgtactca ttccagctga acatacaagc atgattgttt
     9481 tcttttccat tttaaacacg ttcctttcta tacttacact tgtatataag caagtagcgt
     9541 gccaacttta aaagcgcttt aaaaagttga tatcatcaca tttaaggtct ataccaagtt
     9601 gcatacacac taaaaaagaa agcgcacact aaacacacaa attaattttt attacctttt
     9661 ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt ttttcaaata
     9721 aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataagat
     9781 gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca caacctctca
     9841 ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga ttcacaacct
     9901 cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc tatttattta
     9961 tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata ccacgaagca
    10021 gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa tccatatcga
    10081 ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat atagccttcg
    10141 caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc gataaattcg
    10201 atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc atattctctt
    10261 cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt tagatacatg
    10321 gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt cattttcttc
    10381 ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc ttcattccat
    10441 tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag gaaatctact
    10501 gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat cgattgtttt
    10561 tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc aaaaaatact
    10621 cgattataaa acaaatcata catataagca gctttttggt cttctggaga attggatcga
    10681 gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt tccgctgcgg
    10741 taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat catccacttt
    10801 tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg
    10861 attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc aaaggcaatt
    10921 tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc atatttttcc
    10981 aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac gtctgcttcg
    11041 atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac tatacagttc
    11101 tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt cagatggatg
    11161 gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc tacggctggg
    11221 ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat aaacgaatcc
    11281 tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc agcaccaaga
    11341 ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga tcccatcatt
    11401 ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa caataataca
    11461 aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag cattgacgat
    11521 cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta aagcggttgc
    11581 aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag ctaaaattgg
    11641 attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga tcccaggagc
    11701 aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca atactgcaat
    11761 cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc aaaatggcat
    11821 agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta cagcaattgc
    11881 cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga taccaatcac
    11941 ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt gtacgacacc
    12001 ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc ctgtaagttc
    12061 tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc
    12121 agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct gtagtttctt
    12181 ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa
    12241 taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa cagtctgatt
    12301 ttcattcact aattcataaa cagttggatt aacagataaa taagacatca ctgcgatgat
    12361 accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta tcagaaaaac
    12421 agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga taggctttaa
    12481 ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt caaagaccat
    12541 tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg cagcaaatgc
    12601 gttttttatc accatgatat accttaaatt attgattctc gttgccacgt tccctaaaac
    12661 attggctaat ttatccataa aactcattcc agatcccctc attcctgctt cttctccttg
    12721 acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga aactatttac
    12781 tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca taaaatacat
    12841 cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc tctccgattt
    12901 attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc
    12961 tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat atcataattt
    13021 ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa ggctacgttt
    13081 ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg
    13141 ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac gatttgattc
    13201 ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata tctcagttca
    13261 aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag tgggtcattg
    13321 gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc aaggatattc
    13381 ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc taaataatcg
    13441 gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc ctttaaatat
    13501 ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa accacatacc
    13561 tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc atctgatgat
    13621 tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa gatatttacc
    13681 ataaaaaaga ctaggataaa attcactcct agccttcgat catttttatt ttgaacgaca
    13741 aaaaatatga tgttcaataa cagacgatct tcattcgatt cttgatgtca tcttttgttt
    13801 tttttaatat atggataaaa agtttgatta catcgtttct attctagaat ttggtcgatt
    13861 ctcaattctt cttctggtga gaaatctaaa ttatccatca ttcgaacact ttcttgtaac
    13921 tgacttaacc gacttgctcc gaccagtaca cttgtaacag atttttgtcg caaattccat
    13981 gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg cgattgtttg cagtgctttg
    14041 attttttcca gagttgatcc aacttgttct tctgaaagga atggtatttc cttacgatgt
    14101 gctctagaat cttcaggaat accatgtaga taacgatttg tcagcaaccc ttgagcaagc
    14161 ggactgaacg tgattgcccc tagcccttct tcttctaata ctggagatag atcgtcttct
    14221 agtagtgcac gagaaaacat attgtagcgc atctgatgaa taatgaacgg tgcttccttt
    14281 ctttttaaaa tttcagtcat ttttttgtat cttctccatt atagttggat ataccaatat
    14341 ataatgcttt tccttgtctt acaagttgca tcaacgcttc tgctgtttct tccaaaggtg
    14401 tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga
    14461 aactttggtc acaacttgat atgatggatt tacgagagcc ccattcccca tatggccctt
    14521 cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat
    14581 cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat
    14641 tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat
    14701 tatggatagg atcataatca ccaaaatttt gccataatcc gagaggagag ataaaatcgg
    14761 taattttaaa cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc
    14821 tgcaatatac aatttttctc ctcctcatca aatcttttta aaacgctttc ttgttttcta
    14881 tttttagttt actacatttt aaagaaaagg agttaatcaa aatgattttt tcgtatattt
    14941 ttttgccatt tgttttttac tcgttgatag atcagaaatt gattacgtgc ttgctggatt
    15001 tttttatagc gtttcggttg ttttttgtaa aattgctgat gatagttttc tgctggccaa
    15061 aacgtaccgg ctggttcgat ttttgtgacg attggttggt ggaaagttcc tgaactgtct
    15121 agtttctgtt ttgattgttc cgctaattct ttttgtcgtt cgtcttgata aaaaatcact
    15181 ggacgatatt gcttccctct atcttgaaat tgtccaaatg tatctgtggg atcagtaatt
    15241 tgccaataga ttgtcagcaa ctcgctataa cttatttctc ttgtgtcata aatgatctcc
    15301 acagcttcca catgacctgt gtaaccacca cttacttgat cataattcgg tttttcaaca
    15361 tgtccgcccg tgtatcctga caaaacagat aggatccctt ttttcgattc aaacggctcg
    15421 accatacacc agaaacatcc ccctgcaaat actgcacgtt cttgatagtc agcatcttgc
    15481 atggcatgct tggaaaagtc atatttttgc tccttatcaa atccttccgt aatcaataaa
    15541 taaaagtcca ttacatcagg cgtcagattc attcggatag ctaatggacg tagtgttact
    15601 tctagttttg ataactgttc tttaacggat atattttctc ttgtgtcatt ctttgtctga
    15661 atcaatactt ttcgttccca atctcgtgta ttcggattca aaatcagatt ataaagtgtt
    15721 tgaatgaata tttgttcctc atccatagat tctcctcctt tccgaagaag acaagctagt
    15781 gactttagct gtaatttatc ataaagtgag gaaataatcg attgaatcgt atttattcaa
    15841 agaagataag cagcttcaga tgatataaga tgatctttat cttaaaaagc atactctatg
    15901 gtttggttta actaaaaaac gacctctgaa aaaagggatg ttctccaatt ctcagaagtc
    15961 gagtaaagat gattcttttt tatgagcgat aataaacttc atccaaaaac agaccttgtg
    16021 cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt
    16081 ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt
    16141 tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag
    16201 ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa
    16261 tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact
    16321 cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa
    16381 cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt
    16441 cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt
    16501 ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc
    16561 cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata
    16621 ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat
    16681 atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct
    16741 gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg
    16801 cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa
    16861 aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt
    16921 ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa actgattctt
    16981 tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt
    17041 ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg
    17101 tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca
    17161 ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca
    17221 tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg
    17281 gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc atacaatgca
    17341 ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt
    17401 acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga
    17461 taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag
    17521 aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt
    17581 ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca
    17641 tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac
    17701 gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg
    17761 cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca
    17821 ttgaatcctg gataacaccg ataataaatc caataccaac gatttgcatc gcgacatcat
    17881 tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac
    17941 ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaatg
    18001 gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg
    18061 caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca
    18121 atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa
    18181 agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt
    18241 taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga
    18301 tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg
    18361 attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt
    18421 gtgacaacgc tatagatatc tgatccacca actcttttgt cggttctgaa cgtgctcgaa
    18481 aagctagtcc caacaataca gcccagaata aaacgccaag gtaattcgca tcaataagag
    18541 attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt
    18601 gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta
    18661 caatgactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct
    18721 tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa
    18781 ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgtccccaat attgaaataa
    18841 atgtccacgt ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac
    18901 gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta
    18961 acaatcattg tatcatttta tctaaagaaa ctaaaggtgc aattttctaa aaattctaat
    19021 aatacaattg gcaattcatg ctcttgcaac attcaatttt agtattggag ggtttattca
    19081 atctctaaag aagtcgagag aaaactattt atcgttatta ttatagttat ccaagtaaaa
    19141 cgtaactaag atgaaggacg aaactagaaa tttcatcctt catcttaatt cttagacata
    19201 gcaatcaatt aatattttcc actgagtctc ttctttctct cactctcact ggtttactta
    19261 cccataaacg atttgattaa agccattttt caattcatta ttcgctgtct cgttaaagta
    19321 aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg gctgattttg
    19381 ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg catcggaata
    19441 gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt ctgcagaaac
    19501 aggcactcca ccaatagcca tcttccactc cccgttcaaa ataacttgag ggactttaag
    19561 accatataag tccacactca tttgattcgt atagcttttg tattgttgtc ccattgtttg
    19621 gccccatgta gccataaatg cctctaattg tgcggatttg tctgcattcc acagtgtttg
    19681 ttcgacttgt tcgctgcttt ctgaagatga agcctctaca gaagaacttg aagtagatgt
    19741 ttgagttgtt tcagtcgttt tggaagactc tgacgaagta gaggtatctg ctgtttccgt
    19801 acttgctgca gttggcttgc tctctgtgct agttgttgta gtagttgcaa ttgttgcttt
    19861 cgctgtttct tttgttgatt cttttttcga ttcattcccg ttggtacaac cagctaacaa
    19921 aaaagctaga ccaattgtta ctaaaatcct ttttttaaat taaaaaactc ctttcgatta
    19981 ctcccacttc tttattctat caaataagca atgtgatttt attgattttt tgtattttct
    20041 catccatctt ttaaaccttt taaaaactaa gaagcacccg ctgatgttgt ctgtaataca
    20101 tcagcgggtg cttcttttat tttaatcttc gcgattttcc aatttacctc acgctttata
    20161 tttgtatttg aagaaacata gacttttagt catcatcgtc ttcctcatcc aaatcttgtc
    20221 ggacagtttt tccttggagt ctttgtactt ggtagatgat ttctttattg aaacttaaaa
    20281 gttcaacaag caattgtttc aagccatatt tttcttcttt ttcagctaat ttgattgccc
    20341 gtgtgataaa gacattttgg aaatcaaaat cttttgcaaa gttttccaac attgttgagg
    20401 ctggtaaata tttgttttga ccatgttcgt aaatttttcc ataacgcatg acttcttcta
    20461 aagtagacgg aacgatttca ttttcaatca ctaactcatg agagatttta ttgaaccatc
    20521 tcgcttcttg atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca
    20581 aaacatacaa ttgtgcttgg atcaatttca tttcttctaa tttaaaattt gccaatacat
    20641 gccccaccat ggcgccagca gttggtttat gatgatcgat atctgcttgt tttgtttctt
    20701 gagcaaattt ttcttccact atattcactg atttacgcct cttttacttt tgttgattgg
    20761 acttcataac ctaatttttc cacaactgca gctaattgtt cagggtggac ttcatctgga
    20821 tcaaagtttg ctttgatttt gctcgcatta aacaaaactt tcgtattgct taccccaggc
    20881 tgttgagaaa ccgcatgttc tattttttgc atacagcttg gacaagctag tgtatctaat
    20941 tgcataatca tttttttcat cgtagttctt ccttcctttt tcttgatagt cttattctag
    21001 attacagctt ccataaaaaa attgattctt atcaagtttt acgatttttc ttctttgctt
    21061 tcgtgtccaa aatacaataa acgcatcgcg ttaaagataa cgatcaagat actagcttcg
    21121 tgaacgaaca tcccactagc catgtaaata aacccggcaa ataagccgat gagtaaaaat
    21181 aagacgacac caatggcaat cactacattt tcacgggtat tcaaaacagt tttcttagcc
    21241 aaacggtaag catgaaccaa cgatccaaaa ctagattgca tcaagacaac gtcactcgtt
    21301 tcgatagcga catccgttcc tgatcccata gcaatcccga tatcagccgc agccaaagag
    21361 ggagaatcat tgattccatc tccaatgaaa gcaacgtgca atccttgttc ttggtatttt
    21421 ttcacaaatt gaaccttttc atctggcaat aattcagcat ggacttcatc aatgcctaac
    21481 atgtcagcta cataatcagc agtcatctga ttatcacctg tcagcatcac taaatgtttt
    21541 gctcctgctt gtttcaacat agctaattgt tcagcgactt ctggtcgaat cacatctgac
    21601 acaccaatga tttgagtgat ttttttgtcg attgctacaa taatgactga gctgcctctt
    21661 tgttgtaatt gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt gtgagcggca
    21721 atcaatttgg tgttgcccgc acaaactgta tgaccatcaa tctcagctgt gattccttgt
    21781 cccttgatcg tctgattatc tttgactgct aattgttgaa aatcaatgtc tttctcctga
    21841 gcataatgga tcacggcgcg ccccagagga tgatcggata acgtttctaa tcgagccact
    21901 aaagctaagc tgtccccttc atcctctgta tagttcttga tagtggatac agcagtagtt
    21961 cctttggtca atgtaccggt tttatcaaag acaaaagtat cgattttgga aaaagtatcc
    22021 attgcttcac cgcctttgac taagatgccc cgtttcgctc cattaccgat tcctgctaca
    22081 ttcgaaacag gcgcaccaat gactaaagcg ccaggacaac caagaaccaa aacagtgatg
    22141 gctaaacgaa aatcacgact gatcagacca acaagcaacg caataagtaa aacggttgga
    22201 gtataatact gagaaaattg atcaatgaat ttttccgcat gtgatttggt atcttgtgct
    22261 tcttcgacta attctataat tttggcaaag gtcgtatcat ctccgacctt agttgcttcg
    22321 atcgtcacgt agccatctgc taaaatcgtt ccagaaaata cgttatctcc actgattttg
    22381 acgacttgtc gtgattcacc cgtcactgcg gcttcatccg catacccttt cccgtcaatg
    22441 actcgtccgt ccacaggaat agctccacca gtttttacta agacgatatc gccttcttcc
    22501 acgtcatcga tttccacttc ttcggtcgtt ccatcttctt ggacaacgct ggcagtcgat
    22561 ggggccatct cggtcaaagc cttgattgat tcccgcgttt tggtcaatgt tttttgttcc
    22621 agataactac caaacaaaaa caagaatgta acgatagctg actccttgta ttctccgatc
    22681 ataaaggcac caagcacagc aatagtaacg agtaaatcaa ttgagataac tttgactttt
    22741 actgcctgat acgcatggat tataataggt atgaccgcaa taatagaagc tatggctaga
    22801 gcaatgttat agaacaagac aaatccagta aatttcccga taaaaccaaa aataatcaac
    22861 acagctgata aggcggtgat atgatttcga tgctcttgga tccacttttg taatttcatc
    22921 tttctccctc ttttcctttt cattcttacg agactaagta tagatactta tccatcataa
    22981 aaaattgacc actatcaagt ttattcaaaa aggcggtttt cttttggtat actgaattcc
    23041 tacagtcatt aaacgatcat atagaaaaag gaagcgccga aacgcttcct taggtagttc
    23101 tattttttta ttcaaagata agatgctttg tcatcttacg actgaagaac aacaatcaaa
    23161 aaagggcagc agccactcat tcacaagaaa tatcgttttt tgatatggga gttgaagcag
    23221 tggacaaata tctctactta ttagctggga ataagatcca aaaaagctta atggatttta
    23281 tccaagaatt ggaatgtact ttccacaaaa aattcactca ttccattctt ttaaaacttt
    23341 tgattcatac agcgtgtcta attgagcgta cattgataaa cggtcatgaa cttaaaatta
    23401 tatcagaaga tgacacaaga ccttctcatg aaacaatttt tcatgtaaaa aaggccttca
    23461 aaaatattga aacagaattt gggatcactg tttcttatga tgaatgtttc tttatctatg
    23521 atattatcgc atctaaataa tcttttttta tgtaaacgag tattttggga gcgtcaataa
    23581 ttttgtgtaa ataaattgtc ctcctgcaaa ataattagtt actcagtaaa cattgaaact
    23641 aatgtatcgg ttac
//
LOCUS       DAFCLG010000033        23575 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFCLG010000033 DAFCLG010000000
VERSION     DAFCLG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.033"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="LND56_001223"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="LND56_001223"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK6743158.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="LND56_001224"
     CDS             complement(1173..1868)
                     /locus_tag="LND56_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBK6743159.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="LND56_001225"
     CDS             complement(1881..2597)
                     /locus_tag="LND56_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBK6743160.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="LND56_001226"
     CDS             2927..3532
                     /locus_tag="LND56_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBK6743161.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="LND56_001227"
     CDS             3857..4180
                     /locus_tag="LND56_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK6743162.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="LND56_001228"
     CDS             4362..5732
                     /locus_tag="LND56_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBK6743163.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="LND56_001229"
     CDS             complement(5862..7163)
                     /locus_tag="LND56_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6743164.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="LND56_001230"
     CDS             complement(7393..9351)
                     /locus_tag="LND56_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBK6743165.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="LND56_001231"
     CDS             complement(9528..9794)
                     /locus_tag="LND56_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6743166.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="LND56_001232"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="LND56_001232"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK6743167.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="LND56_001233"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="LND56_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK6743168.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="LND56_001234"
     CDS             13138..13701
                     /locus_tag="LND56_001234"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6743169.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="LND56_001235"
     CDS             complement(13954..14475)
                     /locus_tag="LND56_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBK6743170.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="LND56_001236"
     CDS             complement(14478..15320)
                     /locus_tag="LND56_001236"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBK6743171.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="LND56_001237"
     CDS             complement(15313..16038)
                     /locus_tag="LND56_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK6743172.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="LND56_001238"
     CDS             16214..16510
                     /locus_tag="LND56_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743173.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="LND56_001239"
     CDS             17078..18670
                     /locus_tag="LND56_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6743174.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="LND56_001240"
     CDS             18683..19423
                     /locus_tag="LND56_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6743175.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19591..20940
                     /locus_tag="LND56_001241"
     CDS             19591..20940
                     /locus_tag="LND56_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6743176.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21068..21658)
                     /locus_tag="LND56_001242"
     CDS             complement(21068..21658)
                     /locus_tag="LND56_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6743177.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21805..23157
                     /gene="brnQ"
                     /locus_tag="LND56_001243"
     CDS             21805..23157
                     /gene="brnQ"
                     /locus_tag="LND56_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6743178.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6754 a         4746 c         4490 g         7585 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaaat tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa
    19561 ttaaactttt ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga
    19621 ctgaaaaata gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt
    19681 ttaggttccg gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt
    19741 actggatgca tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc
    19801 aataaccatt cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc
    19861 actggctgga cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc
    19921 ggaatgtatg tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca
    19981 ttaggtcttc ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt
    20041 tggtttgcat tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg
    20101 atcctcaccc actatacgac agatattggc acagccagta tcacgaatct atggtctcat
    20161 ggcggattct ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt
    20221 gcttttgctg ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa
    20281 gtgttgccgg aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct
    20341 ctttttgtca ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt
    20401 gtagaagtat tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta
    20461 ttaagtgcag cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt
    20521 tctttagcgc aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg
    20581 ccagggaatg ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac
    20641 gtgatgccta gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt
    20701 atctggagta tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa
    20761 gctcattcat ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg
    20821 gcctttgtgt gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca
    20881 cctgtgtggt tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa
    20941 gaaagtagtt gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg
    21001 cttcttaaat ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc
    21061 ctcttttcta tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa
    21121 aaatccaaat accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga
    21181 caccggcaat caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca
    21241 aaactctcac gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac
    21301 caaaagaaaa actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt
    21361 cagggttacg cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa
    21421 agatattcga ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat
    21481 ggttcaccca gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc
    21541 ggatgaccca taaagaatga aagctagatg tattagggat cttgatctct aatacaataa
    21601 ctctcaaaac aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga
    21661 tgattcctcc tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat
    21721 cgttttatat agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt
    21781 aaaaaaaccg taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt
    21841 ataggctcca tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat
    21901 ttgggtcaag aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt
    21961 atcggtcttc ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat
    22021 gaattagcaa aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta
    22081 gttatcggac cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta
    22141 gcaccattta tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt
    22201 tttgtgctgg cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag
    22261 ttcctgaatc ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat
    22321 ccactaggtt ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc
    22381 acagggttta ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc
    22441 atcattgtca caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac
    22501 acgatcaaat ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct
    22561 tatttaggtg ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg
    22621 gctcagattg ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt
    22681 atattagctt gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca
    22741 gatttgtttc cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt
    22801 ttagcggcaa acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc
    22861 atctatccat tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag
    22921 cgtacggctg tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg
    22981 ttgaacgctt gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt
    23041 gcggaaagct acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc
    23101 ggttttgtta tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa
    23161 aagaggttgt gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct
    23221 tctcacattt ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgttta
    23281 ttgagaggtc gtgaaaaagc cgcttagttt caagtattaa gaaatatttt gtgaaaatgg
    23341 cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt gaacaccgtt
    23401 tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa gtagctccat
    23461 atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac aacatttctt
    23521 ttataatata gattttagac atatatatga tataaaaata gttttcgcaa aaaaa
//
LOCUS       DAFCLG010000034        23243 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFCLG010000034 DAFCLG010000000
VERSION     DAFCLG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.034"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(219..500)
                     /locus_tag="LND56_001244"
     CDS             complement(219..500)
                     /locus_tag="LND56_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743179.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
     gene            complement(551..757)
                     /locus_tag="LND56_001245"
     CDS             complement(551..757)
                     /locus_tag="LND56_001245"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743180.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            921..1652
                     /locus_tag="LND56_001246"
     CDS             921..1652
                     /locus_tag="LND56_001246"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743181.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKELT
                     SFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            1706..2851
                     /locus_tag="LND56_001247"
     CDS             1706..2851
                     /locus_tag="LND56_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6743182.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(2936..3241)
                     /locus_tag="LND56_001248"
     CDS             complement(2936..3241)
                     /locus_tag="LND56_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBK6743183.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(3266..3697)
                     /gene="ruvX"
                     /locus_tag="LND56_001249"
     CDS             complement(3266..3697)
                     /gene="ruvX"
                     /locus_tag="LND56_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK6743184.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(3694..3960)
                     /locus_tag="LND56_001250"
     CDS             complement(3694..3960)
                     /locus_tag="LND56_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBK6743185.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(4215..4940)
                     /locus_tag="LND56_001251"
     CDS             complement(4215..4940)
                     /locus_tag="LND56_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK6743186.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(5301..5371)
                     /locus_tag="LND56_001252"
     tRNA            complement(5301..5371)
                     /locus_tag="LND56_001252"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5337..5339),aa:Gly,seq:ccc)
     gene            5488..6240
                     /locus_tag="LND56_001253"
     CDS             5488..6240
                     /locus_tag="LND56_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6743187.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            6256..6735
                     /locus_tag="LND56_001254"
     CDS             6256..6735
                     /locus_tag="LND56_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6743188.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(6986..8566)
                     /locus_tag="LND56_001255"
     CDS             complement(6986..8566)
                     /locus_tag="LND56_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBK6743189.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(8559..9302)
                     /locus_tag="LND56_001256"
     CDS             complement(8559..9302)
                     /locus_tag="LND56_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743190.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(9478..10254)
                     /locus_tag="LND56_001257"
     CDS             complement(9478..10254)
                     /locus_tag="LND56_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743191.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(10502..11338)
                     /locus_tag="LND56_001258"
     CDS             complement(10502..11338)
                     /locus_tag="LND56_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBK6743192.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(11349..12278)
                     /locus_tag="LND56_001259"
     CDS             complement(11349..12278)
                     /locus_tag="LND56_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK6743193.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            12470..12823
                     /locus_tag="LND56_001260"
     CDS             12470..12823
                     /locus_tag="LND56_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6743194.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(12897..13553)
                     /locus_tag="LND56_001261"
     CDS             complement(12897..13553)
                     /locus_tag="LND56_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6743195.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            13779..15713
                     /locus_tag="LND56_001262"
     CDS             13779..15713
                     /locus_tag="LND56_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6743196.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(15799..17022)
                     /locus_tag="LND56_001263"
     CDS             complement(15799..17022)
                     /locus_tag="LND56_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6743197.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(17016..18914)
                     /locus_tag="LND56_001264"
     CDS             complement(17016..18914)
                     /locus_tag="LND56_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBK6743198.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(18908..20026)
                     /locus_tag="LND56_001265"
     CDS             complement(18908..20026)
                     /locus_tag="LND56_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6743199.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(20109..20942)
                     /locus_tag="LND56_001266"
     CDS             complement(20109..20942)
                     /locus_tag="LND56_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6743200.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(20935..21852)
                     /locus_tag="LND56_001267"
     CDS             complement(20935..21852)
                     /locus_tag="LND56_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6743201.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(22079..23203)
                     /locus_tag="LND56_001268"
     CDS             complement(22079..23203)
                     /locus_tag="LND56_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6743202.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7329 a         4561 c         3803 g         7550 t
ORIGIN      
        1 atctatttct tccatttatt cctcttcttt ctaataaatg ttaaaatgta gtcaaataaa
       61 gttttcttag aactttattg tcgagtgttt cctgtggtcg ccaaactgtc gggaacactc
      121 cttttaattt ttttcaaagt atttttatat tttctatact tcatcattat tcaaacaaac
      181 acttcctttt ttctatcaaa agcacgccac gcaacttttt atacttcata ttctttgata
      241 aaccttgcta attcttcttt tctaataaat tgacaatctc cgattatgct tacttttaaa
      301 cctttctctc tgataaattt ttttaatgta cttctgctac agcttaaata cttacaagct
      361 tcccccaatc taaaaacttc tttgcttaga gatgtttcaa ttcgaacttg atctactgct
      421 tcttttgcaa ctcgatagaa atatcttcgc aattcttgat ctaaatccga tggaagaata
      481 acttgaatct cagatttcat tttctttgcc cccactaatt tgactacatg tattcaaatt
      541 caacttttcg tcacttttca ttaaaaaaaa tttgatcaat tgtgatattt tcaattcctt
      601 tttctttcac aagatacttc aaaataatca tttcttctgg agaatagttt gttttaccgc
      661 tttctttgtt tcttaccgct tgtactgaaa tgccatattt ttcagcaagt tcagattgtg
      721 ataatccaca aaactttcta aaatctttag gcttcatgac tcctcactcc tccttctttt
      781 caacttttcg tcactaaaag aataacatat atttattcat accgcaacgt aaaattcaac
      841 ttttatctac tttttcattt cattatatga gaatgtagtg taaactgtag aatagaatag
      901 atgaaggtgg tgaaaccatt ttgtttgata aaaacattaa ttctgaaatt ggaaaacgaa
      961 tacaagaaat acgtgtttct ttagggtata ctcttgaaga atttggcgaa aatctgacac
     1021 ctcccgcaag caaaagcaat gtttctagat gggaaaaagg aaagaacata cccaacaaag
     1081 cgcggctact aagtattgct aaacttggtg gtgtttctac tgaatatcta ctgacaggta
     1141 attctcaaaa aactaagaaa tatatcaagt atttattcaa tgagattttt tttgacaacg
     1201 gtaccttcca taacaaattc aaagatttcg gtaaagacag agataggctt ggtctatatg
     1261 acttattttt agcagtatcg aaaacatata gtctagataa atcaccttct ttaaaagata
     1321 actttgtaga aatgattata gatgatgtat ataaaaaact cttgcaaact gataaatcag
     1381 atgaccaact catcctgggt ttcatatata tttgtcttga tgaaaaactt tcaaacagac
     1441 agaaaagtct taaaagtatc aattatgagg tattcaatta tttaggtgtt gcatttggtt
     1501 taattgatag cgaaatcaaa tctacaaacg caggtaacga tgaagattta ttaaattatt
     1561 taaaagaatt aacatctttt attgatgaaa taaaaacaac tacattcaac aaaaaaaaat
     1621 attttcaaaa tcaatatgat atagaagaat aaagattaca ttttcctatc aaaagcacgc
     1681 cacgcaactg ataggaggaa aaacaatggc aacaattaaa caatatgaaa agaaagacgg
     1741 aaccaaagct tggatgtttc aaacatacct tggaatcagc ccgctaactg gtaaagaagt
     1801 ccgtactaca cgtagaaatt ttaaaacaaa aaaagaagct caaatagagc ttaatagact
     1861 acttgtagac tttgaagaga atggacttaa aagggaaacg aacgaaactt tccaagaagt
     1921 gtatgatctc tggtatgaaa gctataaaac tactgtaaaa gaagtcactc aattaaaaat
     1981 tgagatcaag tttaataaat ggattcttcc aaaatatggg cagtttagaa ttaaagaaat
     2041 tactacaaaa catgttcaga agatattgaa tcagtgggct aaaacaacag atcaatataa
     2101 agtcctacat tctaccgtaa gccgtatttt caaatacgca atgactctag ggctgatcac
     2161 taaaaatccc tgtgagctcg taatcatgcc caacagaaat gttgaaaaaa agaaggatac
     2221 tgtcaaaatt tattcacgag atcaattaga aaccttattt caatatttgg aatccaaaag
     2281 aagtacgtat agggcagagt acgataaaac tttactgcgc tttctatttt ttagtggctg
     2341 tcgaatcagt gaggctttag ccttagactg gtcagatatt gactttgatt caaacaccgt
     2401 cactataaac aaaaccttaa gccaaacaaa atatgggtac aaaatttcaa gtccaaaaac
     2461 gagaaatagc tatggttccc tatctttaga caataaaacc attaattggc taaagaaatg
     2521 gcaaatcaat caaaaaagat atatgttaac cctcgggatt actgatccaa caatgatttt
     2581 ttgtggaatc tacaaagaaa tcattactaa acacgctatc tattctcgta tgatcacaat
     2641 ctgcaagaat tctggtattc catttctagg taaccatgta accagacaca ctcatgctag
     2701 tatgctgtta gagtcgggag tctccatgaa ggaagttcaa gaacgcctta gacactcatc
     2761 aatcaaatta acaatggata cctatgctca tctaactaaa gaaaccaaag aaaaaaccgt
     2821 tgaaaagctt gcagagcacc taaatttcta gcggaacgta ttcaatcccg tattcaattc
     2881 aaaacaaaaa aaagctgaac ccttatctac caagggttca gctatcattt acagcttatt
     2941 cttcttcttc cgccatgaat gtattgaata cttcttcaat catgtcccat tctgcgtctg
     3001 tttcgatttg ttctaactcg ccttctgttc catcttcgtt ttcgatgtat gcataagctt
     3061 gtaattctac atcttcatct tctgaaacac cagcaggata taaaagaaca tagttacgtc
     3121 caaattcttc ttgtccgtca actgttagta ggatttcata taatgtttca tttccttggt
     3181 catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg ggaatggtta tgttctgtca
     3241 taagattacc tcttttcttt tattattaaa ataattgctt acctgagccg tctaaataat
     3301 tttgtaagat catcacagct gctaatttat cgatgacttt ttttcgtttg gctcttgaag
     3361 tatttgcctg ctctactagc atacgttctg cctgaacagt ggttaatcgt tcatcttgat
     3421 aatcaactgg caaggagaat agttctttga tcttttctcc gtatgacatg gaagcttctg
     3481 cccttggtcc gatggaattg ttcatatttt taggtaatcc aactacaaac cggtctactt
     3541 catattgtgc tactaattcc ttcaaccgat caaagccaaa ctcttcttct gcttcattga
     3601 tccgaatgat ctctactcct tgagctgtcc agccaaaggg gtcactgata gcaacaccta
     3661 ctgtcttaga accgacatcc aagcccatta ctctcataaa ttcactccat gatttgagag
     3721 gtagtccttg acaattactt ccatgatctc atcgcgttcg tgacggcgaa tcaaatttcg
     3781 agcatcacga taacgaggaa tgtaggccgg gtctccagaa agcaagtagc cgacaatctg
     3841 attgattgga ttgtagcctt tttcttccaa agcccgataa acaatcgcta atgtttcgct
     3901 cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg gtttcatctg taaaacccat
     3961 gattctacac ctctttcccg caaggtaatt ccagcttacc ttctacaacc ttattttact
     4021 cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc gaataaatta agaaacacta
     4081 tttgtatgtt tttttccttt tttctgtagc tttatcaaaa gtactctttt cataaagtat
     4141 acgctatatt tctttgaatg tatgttttta cacaattttt tcataatatt cataaaaaat
     4201 tcgaatagct gctactaatt ttttgttttt gttgcttgtc ctgtctcata attgatcgtg
     4261 tagccatctt gaacattgta gataaagaca ttgaattcaa tttttttgtc ttcaatgctt
     4321 tctgcttcca attgcactcc cctggctacc agttcattcc cctcaaaatg aggagtgaca
     4381 cggtagcgta catgatgatt tgtttctttg atatatgccg caatatcatt ttcgtgagca
     4441 agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca agtttttaat gttgttattt
     4501 tgaccagtca gctggaaacc aattaaatgg ctgcgattat ataaccattg cccgtcactt
     4561 aatttctttt gtttccaacc tgtcgggtca atatacaaag gctctctctt ttcagtaggg
     4621 aacaaatctt ttccaagcat agcattggct tgtcctactc tgttcaattt atcaatattt
     4681 gaatagcttt cccatgttcc tttggaaagg ctaagatcct cttttgtaaa ttccggctga
     4741 ttcccattga tttctatttc ttgatgtttt ccatcatatt ctggcacatc cgctacttga
     4801 gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct gaaagatatc tgaatctttt
     4861 tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag cgacgatccc cacgatcgcc
     4921 ttggttgttt ttttgctcat ttctttactc ctttactaac agctccctaa atcttaacat
     4981 atgttcccct ataaataaat actagaaaga aatagaaaca gcaaaaaata aaacctgttt
     5041 taagatggct taaaaataca aaaaagagac ggattcctcg tctctaatca gtcaccactc
     5101 tatctgtaag gtatttatta ttattcaaaa attttcttca tactcaagac aatacactga
     5161 tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat ctgcggtaat aagtccatcc
     5221 aagttaaaaa cataagaagt tgtttttgct tggctgttct tattacttgt tgcagagcag
     5281 ctttttcaca acctatttta agcgggtgac gggaatcgaa cccgcgacat cagcttggga
     5341 agctgggatt ttaccactaa actacacccg caatacaaaa acagtatacg cctcttttat
     5401 tccttcctca agttttttta ttctttacgc ttctggtatg ttttgtagta aaataaaact
     5461 aatcaatcaa ttattggaga agatgctatg acgggaaaag aattagatct gttgctggac
     5521 acttgtttac tcgctgggaa aatcatgatg gaaagcaatg cggagatgta tcgggtagaa
     5581 gatacgatga gtcgaattgc tttagcttct ggaaatttcc gtttggtcag ctatgtcact
     5641 caaaccggtc tctttattgg tcttgatcgt acctcgacca tccgaatgga acaaattacc
     5701 aaccgttcga ttaatttaga aaaagtagta aaagtaaatg atctttcacg taaatatgtt
     5761 gcttcagagc taacattaga agaactttac tgcgaactgc aagcaatcga ccgagaccgt
     5821 tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag tgatcagtgg aacaattatg
     5881 gtgttatttg gcggaaagat gaccgattta cctgtgactt tagtcattgg aggattggga
     5941 tacgcagctt atctatatag tttgaaattt tttagaatca aatttttatc ggaattttta
     6001 tcttctttat tgattggcat agccgctatc tacagcgtac gttttggctt aggaagcaat
     6061 caggatctaa tcattatcgg atgtgtgatg cctttagttc ctggagttca aattacgaac
     6121 gctatccgtg atttgctagc tggacattat gtctcaggag tttctcgcgg aacagaagcg
     6181 atgatgactg ctacgatgat cggttttgcg attgctttta ttttccagct tttttattag
     6241 gaaaggaaca caagtatggg gacgatattt attcagtttt cttttagttt tttagcgact
     6301 gcggcatttg ccgttattac aaatgtacct aggcgttcat tgatttgttg tggtctttct
     6361 ggtgcttttg gctggatgat ctactggatc tgtgttcaaa taggcggatc tgctgctttt
     6421 ggttcattgc ttggttcttt aggagtagca ttcatcagcg atcttttctc taaacgtttg
     6481 aaaatgcccg taacgatttt caatattccc gggatggttc ctctagtccc tggtggttta
     6541 gcttatcaag cggttcgaaa tctcgttact gaatcctatc gagaagcggc tcattatgcg
     6601 gtccaagcaa ttatgattgc tggagcaatc gctttaggtc ttgtattatc agaagtcttt
     6661 aaccataaca tccgtaattt caaagtaaag cgtgacgcaa tcatgatgaa acgaaaaaaa
     6721 cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg tgaaagagac gttcagcctt
     6781 gagcgatgac aaaatatctc acaaacattg cgacaagtaa ccgcaggagc aataagaagt
     6841 aattatgagt attaaaaaaa gttttgtaaa aatagcttct cacattttta cataaaattg
     6901 acttaatact gagaaactga cttttgaaca ccctctatct gtaaaaaagg ggctgcgaca
     6961 tgatttttgt cacggcccct cttctttatt caaatttttt ccaaaacgtt cgttgataat
     7021 aaacgatcca tagagcggaa agtattagaa ctaaaccgat cacaggaata ttcactaacc
     7081 aaaatggata aaacaaagcc aacattccgt ataagacgat aagaataata ctgatgatca
     7141 atgaacccat catagttgcg actaaaccca aacttcctga acctctgtta aataattggt
     7201 taatattggt ccagttcagc agcagcaacc gataatctct agcgaaataa cgtagtgaca
     7261 ataagtagca gccgaataaa gaaccaaata gcaagctagg gataaaaaac aaaggcagct
     7321 gaaaagacag tccgcttaat aatgcgatcc caccagttaa aaaactctgc aatatccatc
     7381 cgaatctgaa cttttctttt aaatattggt tcatagagat tggcaaagaa cggataaaca
     7441 gaaaattttc ttgatccaaa gaaataagat tgctgataaa agaagtctga ttaactgtta
     7501 agaaagccaa tgcgatccct accaggaaaa caacaccgat taacttatta tctaaatcag
     7561 ttaattttat ttcgcctcct agggcaaaag caagaataaa aatgattgga gttaacaagg
     7621 aagaagaaag aacttgcata atcaaattag gttcttttac taattgagca ttgtaactaa
     7681 ataaaagctg atgcacattt tggttctttt tatgggttct ttttattttg aactcactgt
     7741 ttgaatcagt cagttgctct gtaagctttg gtactatcag ctgttttaca gcaaggaaaa
     7801 atcccagagc cgtgatagct agtattcccc aacccaataa accttgttga gaaagtggct
     7861 gactggcagc atcatagaat ggcaataata tcgaaatcgg atgccgatct gttaactcat
     7921 tttcaatagt ccccgtttga tgattcatcc acatgattcc agcaaatgcg atcagcatcg
     7981 agctgccaag caagatacta gaaactgttt ttctatgttt tttaaacact gctgttcgag
     8041 ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat aatcaaaaat agagaaaaca
     8101 aaaggattga gataatgata gtaagcaaaa tccctagatc agctcgccat ccggttaaaa
     8161 taaacatgac tagaagcgga aaaacaaaag gaatgatggt caaagcaact accaaaatct
     8221 ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa aaatacttgc aggtcatttc
     8281 cttcaaaaaa gatattataa atgactgata tactttgagt taaacctaac ataccaaaaa
     8341 gtgccacgta ataagtaaag aaacctggca atttaacaaa gttcatagca aacatagcaa
     8401 atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt ttgagacaaa agatagatag
     8461 ttaatcgctt tccctttttt cctttctctc ttgctttatt cgtgagctgt ggattaacat
     8521 ataaaagatt aactcgaatt aattctaata attgtcgctt atacatacgg tccatctcct
     8581 gtcgcttgcc ttccagccat tttcaaataa atcttttcta gcgtttcatc aggtgagcca
     8641 ttcaataatt cactaactgc cccctgataa atcaattcac ctttcttcaa aatagccagt
     8701 ttatcgcata actgttgtgc ggtatccaat acatgtgtag aaaaaatgac tgtttttcct
     8761 tttgcggcat gagccttcat catttctttc aaatcaaatg ctgcttgagg atcaagtcct
     8821 tgcaaaggtt catccaatac ccaaatatct ggatctggca gcaacgtgcc gatcaaaatc
     8881 gttttttgac gcattccatg tgaaaaactt gcgattgttt cattttgatg actatacata
     8941 tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt tttgtgtgtc taattcatag
     9001 gcagctgaaa tcaagtccca atattctcca gccgtcaatt gtaaaaaaat atctggggaa
     9061 tcaggcacat aaccgatttt ttgtttgata ctcaatcgat tttccgtcaa tttcattcca
     9121 tctactaaaa tcgttcccga tgatggttca ataatactaa ctaaactttt aatcgtagtt
     9181 gattttccgg caccattatg tcctaaaaat ccaaaaattt ccccttgtct tatcgagaga
     9241 gacaagtctt ttaatgcttg ttttttcccg taacttttac ttacattgat gaattcaatc
     9301 atttctgtac tccttttgtt tgttattact ttttattgta cccttcgtta ttggtggagt
     9361 aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg actaggctgc tacttgggtt
     9421 gttctcttct acaggaaaac actcttctaa caggtaagat atgagtcttt gttttcttta
     9481 aaatgattca ttttctatct tttcttgcat aaaattcttt agtttatcaa tacttctctt
     9541 tcccatctct agttgttctc catttgcaaa tgttactatt tcgtttactc tgctaataga
     9601 tgtaatgttt tcagtattaa tgatataagc ttttaaatct ttacataaat actcatattc
     9661 gagtgatttt ttatattttt taattgtgcc gtttacgaga atctcttcat ctttcaatac
     9721 taggcctatt ttatttttat aacctggcat gctcattaga taaaggattt catcgtaatg
     9781 gatatagtga tcgctattac cataacggac agttgtccac ctttttttcg tagcatgttg
     9841 aacaggatag ttttcttttt ctttcaataa gtaactttgg atgctttttt tatttttttc
     9901 ataagtgaat tcttttatca aataaccact aggcgtaatc tttcgattaa taacagttat
     9961 tgctttattc ggatgagacg ttagataaac aattttacac ttttcattct tttctctaag
    10021 catttcgcct agttcgatac cgctataata ggcgttcaaa tcgatgtcta ggaaaaaaat
    10081 atctgaggaa tagatttcat tatttttaat gaattcaaca aattgtataa cgttcaatcc
    10141 ctgaacgatc tcaaatttcc aaggacttaa taaaattctg gattcttcca aaatttctac
    10201 tagcattttt tcaaaaacca aactatcatc caaaataaat atacgcgtta ccataaaaag
    10261 tacctccact cgatatttta ctatcaaaat catttcatac aacttttcaa catacatgtt
    10321 aggaataaat tgtaattctc ttcacaatct tttccaacac ctttgaaacc gatatcaaat
    10381 actttcagga tgctgcttct aaaataaata tcaaacaaat gaatacatca tgcaaaaaaa
    10441 gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt attttgttct
    10501 tttaaaaaga acgatacaca tactgatttt tcggagcaga gactttctca gcgtttgtaa
    10561 caactacaga tgggatttca cgcttgaatg gttgataata gtctgattgg atggttcctt
    10621 ctattttata ccattcatca tttttaaccg ttactccttc cggcatatgc gtcaacaaac
    10681 caaagactcc tgaatctgcc acacaatgga taatgccgaa tcggaacaag aagatatctg
    10741 tattgctatc gttcggtgtt tggtaaacaa atccttcata gctgattttt ttgccaatga
    10801 attcgctagg gtaattataa attagctcca taacttctaa atagttttca tctgtgatct
    10861 tgatagtttg cttatcccca tacttttctt tcaactcatt catttggtct aagtagtctg
    10921 atttgttgaa ataaatactg gtatccggct ttaagtattg tgtctgcatc tccggatccc
    10981 ctactgattc tttacttact gggaaattaa atcctttggc ttctacgatc gtagtgtcga
    11041 gactaactgt tggaaacaac gtccccacga ttactggcaa agctagcagt acataagcga
    11101 ttccacgttg ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt gccttttctt
    11161 ctgcttttcc gtttttgacc cacaaataca gctgaactaa agccaaaaga gcagataaaa
    11221 tcattgaaag aatagctaag tagcgataat ggacattgat atattgatcg agtttgccag
    11281 aaacttgcaa gtacatcatc aattcgaaat acccagataa aatcaaaaac cgcagcatat
    11341 tttccctcct aaataaataa tgtgaatcca ataacgctaa gggcaatcag tccgatcagc
    11401 gataaaataa agcgtcctgt aaaatatcgt ttcatcatca gtagattttt gatatcgacc
    11461 attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact taacaaagaa
    11521 gatcctataa acgcatctgc ttctgagcaa agagacatcg taaaggccaa taacagcatg
    11581 atgataatgg ctaaaatctt tgttgagccg agcgttagca taattctagt tggtaaataa
    11641 gtctgcatcg cagctgcgat taatccgcca atgatcaaat aacgacctgt atcaaaaaat
    11701 tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga tcagctgatt ttttggttca
    11761 ttcttctcac tttgttcttc ttcatggaca tgatgatggt gattttctgc tactttagac
    11821 gtgatttttt ctttcaatac agcgtctttt tgaaaatagg caagccagac accaatcaca
    11881 agggcaacaa aaaagctgcc tatcacccgc cagcttgcca tctgccaaga attgccaaat
    11941 gcgataaaag tggaaaaaat cacgattgga ttgatgatcg gagcagtcag cataaaagca
    12001 aacgctgtgt gaactggtac gcctttttta acaaattgat gaacgatcgg tacgatgccg
    12061 cattcacatg aaggaaagaa aaatcctaag atcgaaccag taaaaataga gaagatacga
    12121 ttttcaggaa gccatctctt cacacgttct ggggtcaaaa atacttgtaa ggcgcctgag
    12181 ataagacagc ctaataatac gaaaggtaat gcttcaataa cgatcgataa gaaaatcgta
    12241 ctcatctgca aaaccgaatt tggaataaaa gaaaacatga gtttcctcct ttttatttct
    12301 agttcattcg ctttgcaaat atacgctttt attcaaaaaa aacaagttgc tctgtaaatt
    12361 tttacacaac tcttattttt taccaaagca cttcaattga ctttttctgt tattcccatt
    12421 aaattaaggt ctcatacata tcacttctat cataaaaggg agaataaaaa tgaaacaaac
    12481 cactaacttg actgaagttc aagacatact gcaacagtca tccgttgccg tcatttatct
    12541 ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca cgactagagg aacttctcac
    12601 acattatgat attccggcac ttcatttgga tgcatttgaa acaccagaag tagctagtaa
    12661 atttgaagtg ctgactgcgc cagttgtcct gatttttcat caaggaaaag aagtctttcg
    12721 gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac tgacagcaga
    12781 agatgattcc ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt ccaaacagaa
    12841 aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt caatcgcttt ttatttttat
    12901 tctgctgtat tttcggaatg cgcatttgcg tcagaatgca agaaatagat caaggtttgc
    12961 aattcattcg tcaaatcaac attttggatc aatacatctg acggagcatt caatcgagca
    13021 gcggtaaagt tcaagattcc tttgattcct gcttccgtta actgattgac tacttcttgt
    13081 gctctttcag cgggtatcgt taaaattgct acttcgattt gttggatctt gatttgttct
    13141 gacatatctt ccattggata aacggggata ccgtctacga tgcgcccaac gatttcctca
    13201 ttcacatcaa aagcacaact gatacgtatg ctattacttt gatggaattt gtattttagt
    13261 aaagcacttc ccaagttccc tacaccgatc agcgcaacat ttgtcaattg atcgtcgttt
    13321 aatgtctttg caaaaaagtg cataagatct tctacatcat aaccataccc acgttttcct
    13381 aattcgccaa aataagaaaa atctcgacgg atcgttgcac tatctacttg aacggcttca
    13441 cttaattcag ttgaagacac ttttttcttt ccagcattat ttaaaatcct taaatagcga
    13501 taatacaaag gcagtctttt agctgtcgcc tttggaataa tatgatcttt cacgaaatga
    13561 cctccacgta ttgtctaatt acatctttga tttctccatc atagcagtat ttataggaaa
    13621 aaatcaattc cattgctcag atattgtgaa attttattct ttattcattt atttatcatt
    13681 tctgcaaatc tcgcagtata atggaattta tggtagacta ctaattgaaa aatagttttt
    13741 tatttgagga gaattcacaa agtagaaaag aggattccat gatattatta caagccaatc
    13801 agattgctcg ccattttggt gcagaagtct tatttgaaaa catacattta gaaatcagta
    13861 caggcgctcg tgtcgcttta gttggcagaa atggcgctgg aaaatcgact ttattaaaga
    13921 tcattgctgg aattgaagca cctgatgaag gaaagatcgc taaaaataaa acagcgactc
    13981 tcggatacct cgctcaggac acaggacttg attccaatga aacagtttgg aacgagatgc
    14041 tgaaagcttt tgaacaagtt agacaaatgg aagagcggat gcgcgagcta gagattttca
    14101 ttagcgaagg tacgccagac acacctgctt atagttctta tttaaaagaa tacgaccggc
    14161 tgcaacacga tttttcagat cgaaacggct atggttatga aaatgaaatt cgttccgtat
    14221 tgcatgggtt taaattcgat tcttcatttt atgatcagaa aatcgatact ttatcaggcg
    14281 gacagcgcac acgtttagct ttagctcgca tgcttttgca acaccctgac attttgattc
    14341 tagatgaacc aacgaaccat ttagatatcg agacactttc ctggcttgag aattatctgc
    14401 aaagctatac tggtgcatta ttgattgttt cccatgaccg ttatttttta gataaagtag
    14461 tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt aactattcga
    14521 aatatctaga tttgaaagca gaacagttag caagtgaatg gaaggcttat gaaaagcagc
    14581 aaacagaaat ccataaatta gaagacttcg ttgctcggaa ccttgttcgc gcctctacca
    14641 caaaacgagc gcaaagccgc agaaagacat tagaaaaaat ggaccggctg gatcgtcctc
    14701 aaggggatga aaaacaagca aactttctat tcagtataga aaagccttct ggaaatgtag
    14761 tcttgcaaat cgaagatgcc gctatcggat atgataatgt caccttatct gaaccaatca
    14821 atctagatat ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt atcgggaaat
    14881 cgacgttgct gaaatcaatc attcaccaga tcccttttat ccgtggaaaa gaaacgctag
    14941 ggacaaatgt caccatcggc tattacgatc aaggtcaagc tgatctgcat acaaacaaga
    15001 cgatcctagc agaattgtgg gatgagcatc ctaccactcc agaaaaagag atccgaagtg
    15061 ctttaggtgc tttcttattt tctggcgaag atgtagaaaa accaatccct ctgctaagtg
    15121 gcggtgaaaa agcacgggtt gctttagcca agttagcgat gaacaaagaa aattttttga
    15181 ttttggatga accaacaaac catttagata ttgacaacaa agaagtatta gaaaatgcat
    15241 tgatcgatta tgacggcaca ctgctttttg tttcccatga ccgatatttc atcaatcgga
    15301 tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta ggagactatg
    15361 attattattt agagaaaaag caggaagaag aagaaatcgc cgaattgttg gcacaagatg
    15421 agccagaatc tgctcctgct cccacgaaca aatctgatta ttaccaaagc aaagaacagc
    15481 aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa atggcgcgga
    15541 tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc gccagaaaac ctggaagatc
    15601 acgtaaaact caatgagctg aaccaagatt tagaagctgc tagacaagat caagaaacaa
    15661 aattgacgga atgggaagaa ctaagcttgg aattagaaga atatgaaaat taacgaaaaa
    15721 accaaaacag aaaccattca tgctgttttg gtttttcttt ttttatttta tcaaacaaaa
    15781 attccatatt tacctttttc accaataaga agaccaatta ctagtcattc tcactaaagc
    15841 ttctaaataa tagtaatcac cccaaataca aggtgtattt accccattat ttttgggctt
    15901 attataaaca gactgtgtta aaaagccatc ttcttcttcc gttgtagcat atctttcttt
    15961 caattgtcgc ataattttcc aaacagcaga ctggtataag ttcttttctg ttttgtctaa
    16021 tagacctgat tctattaatt ctaataatcc acaaaccatg atcaccatac tagatgtatc
    16081 tttttcttct ctgctagaat catcaaagat caaatcccaa taacaaatgg tatcttctgg
    16141 caaccgattc ataaaataat tggctaatat tttgcttaaa tcaaaatatt tttgttcttt
    16201 caaataacga taacttaatg caaaaccgta aatcccccat gcttggcctc tagcccaaca
    16261 ggaattatca ctataacctt gatgcgtact tccatatctt ggttttccat ctttgacatc
    16321 catataaaat gtatgataag ttgaaccatc ttctcttact aaataatctg ctgcattttg
    16381 aatatgagcc tgagcagctt ttttatattt ttgatttcca gtttcttcac tcgcccaata
    16441 caataatgga agattcatgc aacaatccat gatcattcta ccttgttgtg aaggatcact
    16501 aggatctccc caagcttgta taactcctgc cttctcccaa aaacgctgaa gtaatgtttc
    16561 tgcagcatca attgaagctt ccttcatttt tcgatttccc gttattttat aatctgctac
    16621 ggttgataaa gaatacaaaa aaccaagatc gtgatgatta gtgataattt gacagtcaat
    16681 cctgtggata aaatctatat ctgctttttc agctgtttgt tgaaataaag gatcacccgt
    16741 cttctcaaat aacaaccaat taattcctgt ccaaaatcct tctgtccaac caatattgtc
    16801 tcttaatgga taacgtcctt ttataggaca ctcgcttggg aattgatact gactgttact
    16861 gatacgtttg attaacctct ccgtgtgtct gcgacaacgc tcgatctcat tattccattc
    16921 tttttccatt aattctttat aacattcatt tatccatttt tctggatgta ttatctcttc
    16981 aaacatcact ttatcaagca tttccccacc agcctttagc ataattttag tcttttttta
    17041 ttgtttttat tttggatagt tagcccggtt gtcccatcaa aatcaatttt gggacgattg
    17101 ttccatactt ctaaactata atcccgatct tgatgagcaa ccactcctgt tccgagccaa
    17161 tgggttcctt ttccaaatgt accctctaat ccaggaataa aagtgttttc ccatgtcatc
    17221 aagtttgtat tgggcaaacc cataatagct gtggattttc gtttaatagt atcgtcactt
    17281 aaacagataa tacctgaaaa gccagcttca ctcaccctat agttttcctt gtcttcttct
    17341 atgattattc cttctttctc aggagaacat tcaatagcaa accctgtctc atatagaaca
    17401 tattctgtat ctaattctac tttgtgtacg cgaatctgcc agccttcaaa aggaataagc
    17461 caagttctga tttttgcctg taaagtaggt tcccatgtag aggcaagtac ctctttctta
    17521 catacaatgt ctcgtacatt atttcgaacg atgaactgtc cataggtttt gatgctctca
    17581 ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat tgaatccatt
    17641 gccatttctg ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg actagaataa
    17701 gcaaatttta aatatttttc attggtcaat gtatggaaat ttggccctaa ttgtcccgca
    17761 ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca aattggcttc tgaaagaagt
    17821 ttagttcctt cacgaatagg cataggcatt tctttagccg tccaatactc atggtcttca
    17881 ggtagcgcca acaataaaaa aatcttattc agccataatg gagataacgt actattatag
    17941 ggttcagtca atgtcaattg agggtaggta taaccaatat tcaggatacc tttttcatca
    18001 aatatttctt tttgcatcca ccatcttata ttccgattga ctaacccctt gatttcacct
    18061 aatttaaaag gcaaaagatc ggcccacaac atagctgacc aaaagcttga aactgcaaaa
    18121 cgataaatca tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa aaaatgaata
    18181 tagtctctag aaaaatcaat agctcggttc ttatatatct ggctaatggg cttttctggt
    18241 tctagtacgc tataaatcaa accataataa tgaaatgcaa aaggattata ataatcttgg
    18301 cgacctcttg aactgtctcg ataccagcca ttttgaagat aacaattttc tatattatca
    18361 aatgcttctt tcagtgctga ttctttaact ggataaccaa atttttggat aattgttccg
    18421 acaattatct taaaaaattg ccaattacca tctgcacatt catattcaaa aatttgcgag
    18481 aaccaatgaa taatctggct tttttctcct gtatctaatt ttttccataa taattgttca
    18541 tgatgatata acgctaataa cattgcgggc atttctacca tacgctggtc tctttcatgt
    18601 atttctcccc aataatcggg atgggaagga tctgttccag cttttattcc tgatagaatt
    18661 aatttcaacc atcgttcatc taattggtca attgcgggcc ccgctcccca taaaacccga
    18721 gagaatcctt ccattcctgc aattttattt ccatatccta ccccttgatg tatataatca
    18781 atttgtgcgc cacttgctcg ataaaacggc tggagtggtt gatttatctt tttaaataag
    18841 tcaaagtaat cctgtctggt ccttaatagt ggtactggat gtttcattgt attttctcct
    18901 ctttttacta ccaaatagct aattccgact gttctaatcg aagtaagcct tcaataaaaa
    18961 aataatcaga atagatcagt ggcacattat aagtcagttc tctatggtat gcttctgctc
    19021 cgcctccaat gatcccatct tcatccatat tccaatttgc atagtgcttt tctgtctttt
    19081 ggagtatatt gatagcagct tcatgataca tttttgatcg tacagaggaa acatatcctg
    19141 ctaattccag aagaccgcat gcagcgcata ctccagcact ggtatcaata ttcgaatctt
    19201 ttacagcaaa atcaatgaga ggaatataac cagttttttc gattttctga ataaaattta
    19261 ctgctatctt ttctgctata gctagatagt acggattttt tgtgtaacga taacttaacg
    19321 cgaatccata taaagcccat gcatttcctc tgctccaaca agaattctca ttgtatcctt
    19381 gcccgccaag taaccgaata tatttccctg attctgcatc aaattctgcg atatgcccgg
    19441 ttgatccatc tttcctcact agatacttag ctgttgtatc tgcatgctgc cgagcaattt
    19501 tagagaacct cggatcacca gtttccctcg aagcccaata cagtaatggt aaattcatca
    19561 tactatcaat aatcatccaa ccaggtttat ggtcattcca tgctactaaa tagttccctg
    19621 aaatattata gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt cgactatgtt
    19681 tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa cctacatcat
    19741 gatgaacgtc tgaaaaatgg tacagggcag attctaataa ttgttcttgt tgtattgctg
    19801 cctctttgaa cttttctcgt tttgtcttgt gaaagagctg ccataaaata ccactccaaa
    19861 aaccattggt ccaccagtcg attcctctgt cctttattac gtctgtatat tttccgttgt
    19921 aagaaatata tggaatttta ttccccattc tgtctaattc cgcatctatc ttttcagaag
    19981 ctacattcaa aatttttctt gaccattcct tcgtctgttc attcatggat atcccgccat
    20041 tcttttaatt tttatgaatg tggagttgct cctagaacga actagatggt taacattcgt
    20101 tatttttttt acttatttgc agctccaatg tcattctcct taaatttaga ctgcgcccac
    20161 acataaatca agaccattgg gatcactgaa gttgctaaaa ttagcataat tgcgccataa
    20221 ttaattggct cacctgtact agaaaccgtc atatttttga taattaaggg aaccgtatat
    20281 ttatctttat ctgaaagcat gactaatgga aatagatatt gattccaact tcccataaag
    20341 ataagtaaac cactagtaat aatcgtaggt gataaaatcg gtacagcgat tttcgtaaaa
    20401 atcacccact cattggcacc atcgattctg gcagcctcca tcagtgctgt aggaaattga
    20461 ctgctatatt gtcgcatcat aaacactgaa gttgtactgg caatcgaagg gatgatcaca
    20521 cctgcataag tattcgataa tcctaaacga ctcataatgt aaaataaagg aatcaatgtg
    20581 gtaaaaaaag gcaccatcct agatagcata actgtattga acaacagttt attccccata
    20641 aaacgatact ttgctaatgc ataacccgcc attgtagaac tggctgtagc taaaactgta
    20701 ctgattacag caataacaat cgaattccaa ataatcaagg gataattata tctcgccatg
    20761 atggcttgcc agttttttaa tagcatatcc ccaaacttga aattgattcc gccacttaaa
    20821 atttcttgat tactttgcgt tgaagctaca atcatatata aatatggcat caaaaatatt
    20881 aaggcaccaa acacaataat gacgtacgta aaaattctgc tttgtatctt ttgtttagtc
    20941 atttttatca cctccgaatt taaattgaat agccgataat ataacagata caaacaccat
    21001 cgtccaggca atagcagagg cttttccaaa atcaaccatt tcaaaacttg ttttatataa
    21061 attgactcct acgaccaaag ctgaattttc tggtccattg gaagtcccaa agaatatatt
    21121 tgggatattg aaattaccca agccattgat agtggctaaa actgtggcca ttaaaatgat
    21181 tggctttagc atggggatcg ttaataaaaa gaattgccga aatttcgatg ctccatctat
    21241 ttcagcactt tcgtatatct ctttagaaat cccttgaaga ccagctaaaa ataaaatagt
    21301 gttatatcct acactaagcc aaatcatcgt gatgatcatt gatattcgta tccaaaaagg
    21361 atttgttaac cactgtacac cgttaattcc tagcgcattc aaaaatccat tgaataaccc
    21421 agtcggattg aaaaaagtca tgaatacgga agctacagcc accgttgatg taatggttgg
    21481 aatataatag acagttctaa aaaatccttt tccttttaac tgtgctgaat tcaataaaac
    21541 agcaaagtaa atagctaata gcatcgttat tggaattgat cctgccatga taatcaaaac
    21601 attcgtgtaa gatttaataa acaatggatc tcggaagata gctagataat tgtcaaatcc
    21661 aacaaattca aatgagtagc caattttcat aaaactaaaa gcaaacgaga taatgatggg
    21721 aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg accaatccat aagcactttt
    21781 tttatgagac gtatctgttg cttttttctt gctgttcttt attgtcttgc ttggtaacga
    21841 agattgtatc atcgttaagt cctcctaata ttttttaatc accagttaat tttacgccgt
    21901 acttatgagc aaaattttct gcttctttta gatatgaatt ttcaaaatca cctgtttgcc
    21961 aatagttata tgcagcggta gtcaaataag acgtagcatc tgaataagtg gctggtaaaa
    22021 cagtatttga tgtattttca ttgattttat tcaatagttc atagtatttt tgtcctcctt
    22081 agtagtcaaa ctttttctca gctgcttctg tttgataggc caatgagtta gatgtagcca
    22141 cgcccagctc cagtgcgttg gctaagcatt cttcatctgt cagcataaat gtaagaaatt
    22201 gttgtgccac ttgtggattt ttggcatctt tagggacata aaaacttgat cctcctgata
    22261 ctgcaccttt tcctggattt tcttttgaaa aaggaatcag tggagccatg cgccatttgt
    22321 cagatgcatc aggaaaattc aaagacatat tcgtagcata ccagccgcca gcaacaaaca
    22381 ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt acaatatctg
    22441 agtcgatcat ttctttgatt aactttgcag catgtttcgc ttctttagta ttcagacgga
    22501 tatttccttc ttcatcaagc aaagattgat tctgctgggc tagcatgttg ataaacaatt
    22561 cttggtcccc agattctggc aaggcaattc caaaactacc agttttttct tttaatgctt
    22621 ttgccacctg gatatattct tcccaagaac tgatttcttc aaaatctaca cctatttcag
    22681 caaatttttt tggttgataa taacaaagtg ctggggtaaa atcactagcc caaggaataa
    22741 tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt ttgatcataa aactgatcgc
    22801 catatttgtc ataaaatcca tattcatcag cactataaaa agcatcttga aatttttcca
    22861 gcacacctgc tgctgaagtg tctgccatgg agataatatc cggcatatct tcgtgtgtta
    22921 ccaattttgg ggtgatcatt tgtactgttg ctgttcccga agctacattc acaattttta
    22981 agtcaatatt tgctccagtt ttgcggttat agatttttgc agcattttca acatagtttg
    23041 tcagtttgcc gtttgcgaca tatacttcta atgtagtggc tttttttatc attggatctg
    23101 cgatgtttgc tgttacttct tcgttagttt ttccacatgc acttaagctg aataataggg
    23161 taatgtttag caaagctatc accaatttgt atcttttttt catgtctatc ccttctttct
    23221 tttcaagttc ttatataaaa cgc
//
LOCUS       DAFCLG010000035        21422 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFCLG010000035 DAFCLG010000000
VERSION     DAFCLG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.035"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..808
                     /locus_tag="LND56_001269"
     CDS             68..808
                     /locus_tag="LND56_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6743203.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="LND56_001270"
     CDS             complement(1232..1402)
                     /locus_tag="LND56_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6743204.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="LND56_001271"
     CDS             complement(1596..1775)
                     /locus_tag="LND56_001271"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743205.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1785..1961)
                     /locus_tag="LND56_001272"
     CDS             complement(1785..1961)
                     /locus_tag="LND56_001272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743206.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2162..3274
                     /locus_tag="LND56_001273"
     CDS             2162..3274
                     /locus_tag="LND56_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6743207.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="LND56_001274"
     CDS             complement(3352..3645)
                     /locus_tag="LND56_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK6743208.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="LND56_001275"
     CDS             3813..4127
                     /locus_tag="LND56_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBK6743209.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="LND56_001276"
     CDS             4148..6127
                     /locus_tag="LND56_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6743210.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="LND56_001277"
     CDS             6140..6466
                     /locus_tag="LND56_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBK6743211.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="LND56_001278"
     CDS             6482..7000
                     /locus_tag="LND56_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743212.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="LND56_001279"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="LND56_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6743213.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="LND56_001280"
     CDS             complement(8436..9941)
                     /locus_tag="LND56_001280"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBK6743214.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="LND56_001281"
     CDS             complement(10033..10623)
                     /locus_tag="LND56_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6743215.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="LND56_001282"
     CDS             10802..12448
                     /locus_tag="LND56_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6743216.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="LND56_001283"
     CDS             12497..13216
                     /locus_tag="LND56_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6743217.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="LND56_001284"
     CDS             13695..14642
                     /locus_tag="LND56_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6743218.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="LND56_001285"
     CDS             14654..15574
                     /locus_tag="LND56_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6743219.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="LND56_001286"
     CDS             15615..17072
                     /locus_tag="LND56_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6743220.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKTKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="LND56_001287"
     CDS             17417..18031
                     /locus_tag="LND56_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6743221.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="LND56_001288"
     CDS             18024..19748
                     /locus_tag="LND56_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6743222.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="LND56_001289"
     CDS             19745..21196
                     /locus_tag="LND56_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743223.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6907 a         3633 c         4331 g         6551 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac agttcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac catcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaaccaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tc
//
LOCUS       DAFCLG010000036        20706 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFCLG010000036 DAFCLG010000000
VERSION     DAFCLG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.036"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            352..1041
                     /locus_tag="LND56_001290"
     CDS             352..1041
                     /locus_tag="LND56_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743224.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="LND56_001291"
     CDS             1028..2236
                     /locus_tag="LND56_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6743225.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="LND56_001292"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="LND56_001292"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK6743226.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="LND56_001293"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="LND56_001293"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK6743227.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="LND56_001294"
     CDS             complement(6339..7034)
                     /locus_tag="LND56_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBK6743228.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="LND56_001295"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="LND56_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK6743229.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="LND56_001296"
     CDS             complement(8233..8424)
                     /locus_tag="LND56_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBK6743230.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="LND56_001297"
     CDS             complement(8445..9335)
                     /locus_tag="LND56_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK6743231.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="LND56_001298"
     CDS             complement(9322..10089)
                     /locus_tag="LND56_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6743232.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="LND56_001299"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="LND56_001299"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK6743233.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="LND56_001300"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="LND56_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK6743234.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="LND56_001301"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="LND56_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK6743235.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="LND56_001302"
     CDS             14660..15043
                     /locus_tag="LND56_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743236.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="LND56_001303"
     CDS             complement(15211..15969)
                     /locus_tag="LND56_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBK6743237.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="LND56_001304"
     CDS             complement(15987..16805)
                     /locus_tag="LND56_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6743238.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="LND56_001305"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="LND56_001305"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK6743239.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="LND56_001306"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="LND56_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK6743240.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="LND56_001307"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="LND56_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK6743241.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="LND56_001308"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="LND56_001308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK6743242.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5930 a         4220 c         3800 g         6756 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaa
//
LOCUS       DAFCLG010000037        20664 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFCLG010000037 DAFCLG010000000
VERSION     DAFCLG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.037"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..982
                     /locus_tag="LND56_001309"
     CDS             131..982
                     /locus_tag="LND56_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6743243.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            1103..2968
                     /locus_tag="LND56_001310"
     CDS             1103..2968
                     /locus_tag="LND56_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6743244.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            3016..4425
                     /locus_tag="LND56_001311"
     CDS             3016..4425
                     /locus_tag="LND56_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBK6743245.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            4533..5234
                     /locus_tag="LND56_001312"
     CDS             4533..5234
                     /locus_tag="LND56_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6743246.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            5393..5872
                     /locus_tag="LND56_001313"
                     /pseudo
     CDS             5393..5872
                     /locus_tag="LND56_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            6720..7295
                     /locus_tag="LND56_001314"
     CDS             6720..7295
                     /locus_tag="LND56_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBK6743247.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            7295..7735
                     /locus_tag="LND56_001315"
     CDS             7295..7735
                     /locus_tag="LND56_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6743248.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            7751..8452
                     /locus_tag="LND56_001316"
     CDS             7751..8452
                     /locus_tag="LND56_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6743249.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(8940..9365)
                     /locus_tag="LND56_001317"
     CDS             complement(8940..9365)
                     /locus_tag="LND56_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743250.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            9739..10710
                     /locus_tag="LND56_001318"
     CDS             9739..10710
                     /locus_tag="LND56_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6743251.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            10742..12139
                     /locus_tag="LND56_001319"
     CDS             10742..12139
                     /locus_tag="LND56_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6743252.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            12298..12921
                     /locus_tag="LND56_001320"
     CDS             12298..12921
                     /locus_tag="LND56_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743253.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(13147..13671)
                     /locus_tag="LND56_001321"
     CDS             complement(13147..13671)
                     /locus_tag="LND56_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6743254.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(13664..14137)
                     /gene="tsaE"
                     /locus_tag="LND56_001322"
     CDS             complement(13664..14137)
                     /gene="tsaE"
                     /locus_tag="LND56_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK6743255.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(14260..15240)
                     /gene="pta"
                     /locus_tag="LND56_001323"
     CDS             complement(14260..15240)
                     /gene="pta"
                     /locus_tag="LND56_001323"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK6743256.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(15393..16073)
                     /locus_tag="LND56_001324"
     CDS             complement(15393..16073)
                     /locus_tag="LND56_001324"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6743257.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            16199..17068
                     /locus_tag="LND56_001325"
     CDS             16199..17068
                     /locus_tag="LND56_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6743258.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            17094..17609
                     /locus_tag="LND56_001326"
     CDS             17094..17609
                     /locus_tag="LND56_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743259.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(17656..18249)
                     /locus_tag="LND56_001327"
     CDS             complement(17656..18249)
                     /locus_tag="LND56_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6743260.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(18274..18453)
                     /locus_tag="LND56_001328"
     CDS             complement(18274..18453)
                     /locus_tag="LND56_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743261.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(18530..19039)
                     /locus_tag="LND56_001329"
     CDS             complement(18530..19039)
                     /locus_tag="LND56_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743262.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(19135..19515)
                     /locus_tag="LND56_001330"
     CDS             complement(19135..19515)
                     /locus_tag="LND56_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6743263.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            19845..20594
                     /locus_tag="LND56_001331"
     CDS             19845..20594
                     /locus_tag="LND56_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBK6743264.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         6544 a         3710 c         4166 g         6244 t
ORIGIN      
        1 aaaattcgat ggtatgatga ttttgaataa gaaatggatt gttactgtac ggcaggcaaa
       61 accagaattc agctaagaaa cagtaggcga attctaggtt tttttatttt tcttgagagg
      121 gcgaagcaaa atgaaaattg ataaaatttt gaataacaat gtcgtcattt cgaaaaatgg
      181 attcggggaa gaagtcgttt gtatgggaaa aggactagct tttcaaaaaa agattgggga
      241 cgagatttcg ccagaagcgg tccaaaaaga atttgtttta agagattctt ttgctaaaaa
      301 ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg tcaaacaagt
      361 agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt atctgacatt
      421 gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac tgcctaatcc
      481 attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga aagaggctct
      541 tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg gttttattgc
      601 ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga tgaccgctac
      661 cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg tgttcgatga
      721 agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc agcgttatct
      781 caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc aaggaaagta
      841 tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa cgcaaaaaca
      901 ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag tagtaagtga
      961 gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc tggattgaaa
     1021 aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat tttttaaata
     1081 aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc
     1141 ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt tgcgattcag
     1201 attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg acgtgatcca
     1261 agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag cagatgttta
     1321 tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag aagagtccaa
     1381 aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt ttctaggggc
     1441 gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga tgggctggct
     1501 gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg tctttacctt
     1561 cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc agtttttagc
     1621 agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga
     1681 agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata cttcttctgt
     1741 cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg taaaaaaagt
     1801 gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag tgatggttcc
     1861 attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt taggcggtct
     1921 cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg
     1981 gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt ttttgaatat
     2041 cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct tggcacaagg
     2101 tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag cattagggat
     2161 ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg gtgtgacatt
     2221 acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg gagctattat
     2281 cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga cgattccgac
     2341 tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg
     2401 aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg aacaaacaca
     2461 agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa ccgattactt cagcacggca
     2521 taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac cagaccaagt
     2581 tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag gagaactagt
     2641 tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg tagggatcac
     2701 aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag aattaaatgg
     2761 gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag atttattgat
     2821 tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc cagttgtcat
     2881 tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga ttattgcaaa
     2941 cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca cgtttttcag
     3001 aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc ttatgatcga
     3061 gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg ctttgcgatt
     3121 atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta cccgaatcat
     3181 cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga
     3241 ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg agacgaaggt
     3301 actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg cttaaaatat
     3361 gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa
     3421 tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc tcagacagta
     3481 ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat caattcggcc
     3541 tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg agagtaccaa
     3601 aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt aaaaatcggc
     3661 cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac aacttacagc
     3721 atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga attcaatttt
     3781 ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg tacccataaa
     3841 aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca gttgctaaaa
     3901 gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat caatgagact
     3961 gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa tccgtttctt
     4021 gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc attgaatgaa
     4081 ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg agccattgat
     4141 catatggaag aggatggcac gattattgat gattatcgga tcgattattt acgtcgtcat
     4201 atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta tacgccttgg
     4261 ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta cggctttatc
     4321 tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa aaaatctttt
     4381 gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat
     4441 gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt atgtgctatt
     4501 cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta ctatacttgg
     4561 aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc ggttcgatgt
     4621 tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact gtccgaaagg
     4681 caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt gggacgacgg
     4741 ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg caaatcttgg
     4801 agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc catataaaga
     4861 aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag cgcttatatt
     4921 atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa atgatgcaat
     4981 tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa aaaggatata
     5041 ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa gaacaaggaa
     5101 ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct acgcaaattg
     5161 gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct taccatattg
     5221 attatgaaac ataaataaaa aaagaggttg tgaaaaagct gccctgcatc aagtaataag
     5281 aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat taccgcagat
     5341 acacggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac gattttcaga
     5401 aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc cattcggctt
     5461 gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atactcaatc gtcaagaaat
     5521 cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg gatgtttccc
     5581 tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg ggacagtagg
     5641 tggcggatta agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc
     5701 agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg gttcttttgt
     5761 cacaggaata accattttat tattgaaaaa accagtagat ccgaatgcag aaatcatcca
     5821 agaagaaaaa gaggaagaag acatatcttg ggatgatttg acaattagtt aggagaggca
     5881 gaatggatta caagaaaaaa gtacaataga tgtaccttga ggcaggaatg gtatgaaaga
     5941 atcagagtta gaaagaatcg aatatacaga ttttggttta ggagcgatcg aaactgtcca
     6001 gagcatcggc accctccttg aaatgacgat gaaggaaaaa tgagaacctt tcgttgtcga
     6061 aagggtctgg tataccttat gtcgaaggaa aggaaacaaa aaaactttcc tgtactattc
     6121 caaaaaggaa agaaagttat tatacagtaa aaaaagaaat tattcttcat gctagagatc
     6181 agtacacgtt cgagcccaat atcaaacact agttccaggc aggcgcaaca ggtgcggtta
     6241 tcactgaatt ttcttccagc agtgacgatt caagtgatat ttttacaaat ccagctattt
     6301 atcgttaaca aaaagatttt taggagaagc tgatgaacaa aaatgatcaa attttgatcg
     6361 tttagttcgt cggctttttt atttagagaa acatcattta tcagtgaaac ataacacgag
     6421 aacaaaatga ttttttcgtt aataaaagaa acaagtattt gttgtttaaa ataatacacg
     6481 ttattggcat ataggacgaa atcccagtat cttctataag taagtcattt catatcgatg
     6541 aaaacacgtt atttattttt gttttatcta taaaatggct gaaaaatgat ttttttctaa
     6601 atactatata tagtggcgtc tatcctatat accactaaaa aactacaaaa tctagtcttt
     6661 tattttaaaa aaacacgtgt agaatgaaga tagacttttg agttagggag tgttttttta
     6721 tgaaacttac tgttttttgc ggttcccgtt ttggtaataa agaaagttac aaatttattg
     6781 cgcaaacgtt aggaaaatac atggcacaag aagaaatcga actggtttac ggtggttcat
     6841 actccgggat tatgggagtg attagtaaaa cagtactgga aaataacgga aaagtcacag
     6901 gtatctatcc gaatgggctt tttgaaactg aattgcctgg aaaagatgtt actacattta
     6961 ttcctacaga aacgatcgat gaaagaaagg tattattatt tgaaaaggga gatgctgtcc
     7021 tcgttttccc tggaggtcta ggtacattgg aggaattctc acaacttctt tcttggatag
     7081 caatcggtct gacgcccgac aagccaatcg gaatattgaa tattggcggt tattacagcg
     7141 gcttgcaaca attattagaa acttttgcaa aagaggattt tatggataaa aaatggttgg
     7201 atcaagtaat cttttccaac aatccattgg aactggtcaa tgaattacga gcagtatcta
     7261 ttaaaaatca gttattattg aaggaggcaa aataatgagt caaatatatt tagcaggacc
     7321 atttttttca gaggagcaaa tcgatcgagt aagtcgtatc gaaaaagcgt tggaggaaaa
     7381 caaaacagtc acaagtttct actctccacg tcatcaccaa gaaagcaatt atgaactatt
     7441 cagcgctgga tgggcgcagg aagtatacga aaaagatatg gaagaactga caaatgcaga
     7501 atttgttgtt gctatccttg actttgagca tcaaacaatc gatccaggaa cggcttacga
     7561 attaggtgtg gcaactatgt tgaaaaaacc gatgatcatt gtacaagaag agacggtacc
     7621 tacgaacttg atgattacac aaagtctcca tacgtattta aaatcagacc aagctgtacg
     7681 tgaatatgat tttgaaacat tgcctgttga aacttacgta ggcgagtact tatagaagag
     7741 gaagggttgt atgtcagtga ttttgatttc aggaacgatt ggtgcaggta agagcagttt
     7801 gacggatatg cttgcaaaag agattgattc taaaccattt tatgaaaatg tcgaagataa
     7861 cgaagtattg ccgctctttt attcaaatcc tgagcagtac gcttttttat tgcaaatctt
     7921 ctttttgaac aagcgatttt tagcaatgaa aaatgcttta gtgaacgatg acaatgtgct
     7981 agaccgatcc atctatgaag atagcctgtt gttccatcta aacgctgatt taggtcgggt
     8041 aacggatatc gaagtacagc agtacgatag cttattagat acgatgctaa acgagttaga
     8101 tgatgttgct ccaaaaaaac ggcctgattt gatggtccat atcaaagttt ctttggacac
     8161 gatgctagaa cgtatcaaaa aacgtgggcg tgactatgaa cagctagaaa gcgatgagac
     8221 gttatatacc tattatgaaa cgttgaacac ccgctataat caatggtatg aagattttga
     8281 tgtatgtcct aaaatacaag tggatgggga taaattcgat tttgtagaaa atccagaaga
     8341 tgcaaaaaag gtgatccgtc agatccaaga aaaattagct gaattggaag gaaagcctgc
     8401 tggaaaatat tttacggata accagcatgc aaaaggtcaa gaattaggct aaaaataaaa
     8461 aaccaaacga cgggaaagaa aaattactac gtgatgtaga ataagccttt ctatcgtttg
     8521 gttattttat attcaaatat ttgaaataaa taaatcttta ctctttgatt tgtaaactga
     8581 aatgatccac tgtttgtttt actgatgaag cttgatcaaa ggaagaagct gagtttgaag
     8641 ttcataaaaa tcagccatag tgagttttgc ttggatttgt ttatcttctt ctacttttta
     8701 cataattttg aacaggataa gattcaaatg ttttaaatga atctttgact agtttgtgac
     8761 agttgtcgca tatgctcaag cttccttggt cattaaattc tgtatggata tttttgttgt
     8821 tattacatgt acaataatca ggactatcga tcatccattt cgtctccttt acaatcatct
     8881 tgcttcttta ctatagcaga aaagaagggg agaaacaaaa gaagatccaa cagacggcac
     8941 tatagactgt agatgttgcc atcctcatcc ttcaaacggt aattaccgat ttttgtaaat
     9001 atgactgttg tttctttttt attttctttt cctgaaataa tatattcgtc atctttggta
     9061 gtgatcttga tatctgtcag cactgttttt tcttcagatg attcagaaga atcagttcct
     9121 tgtgtctgat acgtgcgtgt ccattccatt tttttatctg taaatgtcaa ctgtcccgcg
     9181 gataaatcca tatctttacg tacttttagc gtatatacac tgttggtggt atctgctttt
     9241 tccgctttta agacagaaga atcggttgtt tcgtccgagg attggtcaga gccgccaaac
     9301 aaatcacagc cagtcaatgt caaaagtccc gcagacaaaa taaatagtac gattaatttc
     9361 ttcaaaatac ttcccccatt tgataggata aagaaattat accattggaa tgtaaaactt
     9421 caaacggatg gaagagaaaa acgcacaaaa cacccacaaa tagagtaaat tttagaataa
     9481 aattttaaaa aggcaaaaaa tccgccactt tcagaaagcg ttttaaaaaa gtgttgcaat
     9541 cttttaaaaa aatgctatat taaaaatgaa ccggttcaaa tacttgttta agtataatta
     9601 atagtgagag gaagttgctc tactgaagac tttagttata tggatattga tgacaagcaa
     9661 cactatgcaa agaatgcggt catcataatc gaacaacaag aaagagaaat caaatgtaac
     9721 tggcaggagt gagaatgtat ggcatcgatc cgtgatgtgg ctaagatggc tggtgtttca
     9781 gtgggaacag tttcccgtta cttgaatggt cagcaactga aggaaaagaa catgaaaaaa
     9841 atagcagaag cgatttcagc gttggactat aaagaaaaca tcattgctaa agggttgaag
     9901 aataatcgaa gcttctctat tggacttttg ataaacggga tttccagccg ttttgggtcg
     9961 gaagttgtct cagggatcga aagagtagct gaagaaaatg ggtatagctt attattaagc
    10021 gggtttgccg atcagccaga aaaaattgaa caaaaaattg aatacctgat gaagcatgtg
    10081 atcgatgggc tgattgtctt cttatccgaa gaagagtggt ctggatttga aatgctcaca
    10141 aaaatgagta ttccagttat cgcattgaat tgtccagaag gaccagcagg tattgataca
    10201 atattagtga atgatcgaga aagtgtagca aaagtgatcg ctcatcatgg tgaacaagga
    10261 cataaaaaaa tcggatttat tgctgccact caaacagact acgtagcaag agaacgttta
    10321 gcaggagcca aagaagcggt ccaaaatgat ccatcgatcc aattagaggt ctttacagga
    10381 gactattcta gaaaaagcgg ctattacggg gcaaaagcat tattagaaaa aggggcaact
    10441 gctatttttg tcagcaatta caatatgtcg attggtgcca tcgaatgttt caatcaagaa
    10501 ggtgtcagaa tcgggaagga tattgcgttc agtcactatg attatttaga taaaaacgat
    10561 ctgagtatcc ttccgaaaat cacgatcacg ccaccaacag ataaaatcgg ggaacgagca
    10621 gcagaacagc tgttaaaacg aatcaaaaaa gaagaggaag aaaatgaaaa agtgattgtg
    10681 atggaaaata tcatacatgg aatcaactaa aacgaactaa aaaagaaaaa gaggtgcttt
    10741 gatgaaaaaa tatcaatttc cagcaaattt ttggtggggt tctgcagcaa gtggcccaca
    10801 aacagaagga aggatttcag gcgatggaaa aggagagaac atttttgatt attggtacaa
    10861 aaaagaaccg caaaagttct ttgatcaagt aggacctgaa aaaacctcgc aagtttatac
    10921 gaagtatcaa gaggatgtac agttgatgaa acagactggg cataattcat ttcgaacatc
    10981 tatccaatgg agccgtttga tacctgatga atctggaaca atcaacgaag aagccgttcg
    11041 tttctatcat cgttattttg atgagttgat tgaaaacggt atcgaaccct ttgtgaatct
    11101 gtatcatttc gatatgccga tgtatctgca agaaaaaggc ggatggttga accgagaaac
    11161 ggttgaagcc tatgaaaaat atgccaaaag ttgttttgaa ttgtttggtt cgaaagtaaa
    11221 aaaatggttt actcataatg aaccaatcgt tccagtcgaa gcaggttatt tatatcgatg
    11281 gcattatcca gaagagtcag atatgaaaaa agcgatccaa gttggctatc atgaagcgtt
    11341 ggcttcagcg ttagccatca aatcttacca tgaaatggag caaggtggtg ttattggtat
    11401 catcttgaat ctgacaccaa gctatccaag agacgaatca aaccctgaag atgttaaagc
    11461 agcgcaaata gcagatgcgt tcttcaatcg ttcattcctt gatccagctg taaaaggcga
    11521 gtttccaagt gaattgattg agatagtaaa agaacttgat ctcatgcctg tgatccatgc
    11581 agaggatttg acggttatca aagaaaatac agtagatctt ttaggtatca attattacca
    11641 gccacgccgg gtaaaagcaa aagaaacacc aatcgatact aaaaacggtc caatgcctga
    11701 ggattatttc gacaattatg aaatgcctgg tcgtaaaatg aatccttatc gcggatggga
    11761 gatctatgaa caaggaatct atgatatttt gatcaatgta cgagatcatt atggaaatat
    11821 cgactgcttt atttctgaaa acggtatggg tgtagaaaac gaagcacgat ttatcaaaga
    11881 agatggcatg atccacgatg attatcggat cgaatttgtc caatctcatc tgaaatatgt
    11941 ccatcaagcc attcaagaag gtgccaattg tttaggctat catatgtgga catgtatgga
    12001 taactggtcg tggaccaatg catataagaa tcgatatggc tttatttcag tagacttagc
    12061 acaagatgga aaaagaacaa tcaaaaaatc gggtcattgg ttcaaagacg tatcagataa
    12121 aaacggattt gaggcttaag aaagaatagt tgagagcaaa tgcgtgaaat tctccattct
    12181 taccattttg tgaaaaagaa gttcaattat ttacgatctt tttcaacttt tttgtctttc
    12241 tatttcattt tttgtatata atagaatagt caaaattaaa taggagtgga ggaaagcatg
    12301 aggaaaaaaa gaccgtttct tctcttttgg ttattcggtt ttttcttact gcttatttta
    12361 tccgcagcat tttcaggaaa aatgacagca gatcttgctc agaaagaaac agaaaaaatc
    12421 gtagcaagca aagaatcagt agataatttc aaaaaagaat tctcaactta ttggtgggcg
    12481 gagtataaaa taactgccaa ttacgaaaaa ggcgaattgg acatttattt gccaaaagga
    12541 aaagaagaaa atcaatttat taatatcgat gacgtactta gtgcgatttc tctcagacaa
    12601 tatcaattta aagacaagca tttgaccgtt ttgcttttga ctcatgatgg cgacaaagta
    12661 gcccgtttga aaaatggaaa agtgatcacc gcttattctg aaaaagtaga caaaaaagtg
    12721 gtcgccgatc gtttagcgga atggaaaaag acaaatgaac aagtcaagaa aacaactgtc
    12781 tcttctcaaa aaaacgagtc gacaggaaca agtccttcag attctggcag tggacaaacg
    12841 ataacgggaa gtgaagaaaa tcaagcctca attattacga atgagtcatc acaaggacaa
    12901 tatcaatcct atggacaatg aaaaacagag gttgtgaaat cagcgttttg acctgagtat
    12961 tagagaaaca aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    13021 ggtcagctga ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac
    13081 agtcccttcg tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta
    13141 tttcttctat tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt
    13201 tcttgcccct ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac
    13261 ggctctttca ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt
    13321 cgcccaactg atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg
    13381 cagaatactg ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac
    13441 agatgctgtt ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct
    13501 ttcgtatata taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc
    13561 atccaaaact aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt
    13621 tgcatctcct ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag
    13681 cccatttttc tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt
    13741 tcacataccg cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct
    13801 ccggcagtgc ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa
    13861 aatattcatc taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat
    13921 acaacggaat ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca
    13981 tttgcgttat ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta
    14041 gatcaccagt caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga
    14101 attgttctgt cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct
    14161 ttctttttgc cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag
    14221 aacagcagct gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc
    14281 tgctgtgata atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga
    14341 aaccggtttg ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg
    14401 agcaatttta tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg
    14461 accagctact tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc
    14521 aaattgcaat tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc
    14581 ttcaactact ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat
    14641 tgctactttt ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc
    14701 agccaattct tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc
    14761 ttgatctctt ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt
    14821 tttgatgatc tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac
    14881 cattccgtct gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa
    14941 aatcttttct gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac
    15001 catttcatcc cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat
    15061 tcctctggca tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac
    15121 aagttcttct gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg
    15181 gaaaacgatt ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac
    15241 aagatgacct ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca
    15301 gtttatttta tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc
    15361 gaactgcact ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca
    15421 atcggttctt ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca
    15481 aaaaagccac gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga
    15541 ctcttatcga tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt
    15601 ggtttttccc gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc
    15661 ccgcgatgag aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact
    15721 ccctgtttag cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca
    15781 taaagctctt tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa
    15841 aaagataagc catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact
    15901 ttaacttctt cataaggagt caattccaat gcttcataga tatgatacat atctggatgg
    15961 atcttttggg acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt
    16021 gaaaattctg aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc
    16081 acctcctaat aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct
    16141 cagcacttga ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat
    16201 gatcaaatta attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc
    16261 tcctgaaaat gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc
    16321 tactggtcga aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc
    16381 gatgatcact ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt
    16441 gtcgattcca aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta
    16501 ctatgaagta gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag
    16561 ttttgcttcc atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat
    16621 ggcctcagct aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt
    16681 ggaagaaaag aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt
    16741 attaggtcca gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg
    16801 tcaaaacaat atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt
    16861 cgctcacgag cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga
    16921 tgtcagcatg ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt
    16981 aaaagattat gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat
    17041 attgcgcgcg attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag
    17101 aatttttcat tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa
    17161 aaggacattc cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc
    17221 tttatgccat gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta
    17281 cgcgttttga gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt
    17341 ggcccggtga cttttattat attcgtagac ttcactggac agtccaaggt gatatctata
    17401 atcatcaata tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac
    17461 ttttttcagg agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta
    17521 tttgtattgc tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc
    17581 ctgtttctta ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat
    17641 ttcaggtaca tctttttaga tattcttttg catatgatca taaagatgac cactgatcat
    17701 ccgctgaccg acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt
    17761 ttcatccaca cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc
    17821 ttctaataat cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc
    17881 caaataccat tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag
    17941 ctgatatttc tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt
    18001 ataaccgtaa gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata
    18061 actgtaacga taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc
    18121 ccctgtctgc ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc
    18181 gatttctgga gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca
    18241 ttctaacatg cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc
    18301 agtaatgaaa taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac
    18361 gactggcatt tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt
    18421 ttttccgcaa ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac
    18481 tctcttagct tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata
    18541 tccatacctg acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat
    18601 ctttgactag aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt
    18661 ccttcatcgc tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt
    18721 ctgcggcctt ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta
    18781 tgacttgtcc ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat
    18841 ctttttcaac tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga
    18901 ttgctgctgc ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa
    18961 aagcggcatt gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac
    19021 caaacatcat ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa
    19081 atgaacgaat gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc
    19141 gtgcagttct aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa
    19201 ggtatcttct ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc
    19261 ttcaatatcg tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg
    19321 tcgttgtggc gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc
    19381 taatttatag tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt
    19441 attaacataa aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat
    19501 cgttgtaagg ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct
    19561 ccagttccat tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa
    19621 aaaaggcaaa aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat
    19681 aaatgaaagt aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat
    19741 tatttataat tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta
    19801 aatacagaac acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc
    19861 agtttttagc tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca
    19921 gtcttggctg ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta
    19981 catttatcgg taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag
    20041 tcaacggtat tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa
    20101 agaactattt aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat
    20161 tgttaagtaa agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa
    20221 gctggtttat cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg
    20281 gtcgttatac agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga
    20341 ctgctggtat catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag
    20401 ggcttgctca aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga
    20461 tgttcgtgaa cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt
    20521 ggtataacca aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac
    20581 aacaacacgc ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    20641 tctaaacacg tataaacggc ggaa
//
LOCUS       DAFCLG010000038        20608 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFCLG010000038 DAFCLG010000000
VERSION     DAFCLG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.038"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            295..3684
                     /locus_tag="LND56_001332"
     CDS             295..3684
                     /locus_tag="LND56_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK6743265.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3687..5108
                     /locus_tag="LND56_001333"
     CDS             3687..5108
                     /locus_tag="LND56_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6743266.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5105..6982
                     /locus_tag="LND56_001334"
     CDS             5105..6982
                     /locus_tag="LND56_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6743267.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7074..7916
                     /locus_tag="LND56_001335"
     CDS             7074..7916
                     /locus_tag="LND56_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6743268.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8068..8943)
                     /locus_tag="LND56_001336"
     CDS             complement(8068..8943)
                     /locus_tag="LND56_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6743269.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9150..10181
                     /locus_tag="LND56_001337"
     CDS             9150..10181
                     /locus_tag="LND56_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6743270.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10270..10575
                     /locus_tag="LND56_001338"
     CDS             10270..10575
                     /locus_tag="LND56_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBK6743271.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10754..12769
                     /locus_tag="LND56_001339"
     CDS             10754..12769
                     /locus_tag="LND56_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6743272.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12771..13055
                     /locus_tag="LND56_001340"
     CDS             12771..13055
                     /locus_tag="LND56_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6743273.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13081..14454
                     /locus_tag="LND56_001341"
     CDS             13081..14454
                     /locus_tag="LND56_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6743274.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14537..16552)
                     /locus_tag="LND56_001342"
     CDS             complement(14537..16552)
                     /locus_tag="LND56_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HBK6743275.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16574..17788)
                     /locus_tag="LND56_001343"
     CDS             complement(16574..17788)
                     /locus_tag="LND56_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6743276.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            18009..18953
                     /locus_tag="LND56_001344"
     CDS             18009..18953
                     /locus_tag="LND56_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743277.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19249..20193
                     /locus_tag="LND56_001345"
     CDS             19249..20193
                     /locus_tag="LND56_001345"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6743278.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7041 a         3772 c         3909 g         5886 t
ORIGIN      
        1 ttgtcagaaa tggacagaat tcgaatgatt ttttccatct ttctatagtt gcattccaac
       61 aacctgatca tatgtttttc tccctcaacc cttttctttc aatgaaacgc tcattaatcg
      121 aaggaatcaa cttcataaat gaaaccaatt ggtatgaaaa ttggtgtgat tccctttagc
      181 gcccttcgta taataattat catattaaaa taaataaaat tatgtaacgc ctgtttattt
      241 taataatttt cactattcac gtcaaaacag aaaaaaagaa aaaggagggg aaaaatgacg
      301 accacaggga agaaactgaa agttattttc atgctgataa tattgagttt atcaaacttt
      361 gtgccattat ctgcaatagc agacactaca gatgatccaa cagttttaga aacaatttca
      421 gctgaagtca tttcggatca gtctggaaaa aaagcactga acatcaagct aaatgcgaat
      481 aacaccagtg ctgaaaagat agaaaaagaa attggtctag tcgaaaatta cttaagtgat
      541 gtggaaagaa aagaaggaga tggctatgct tatcaggtaa atagcgggaa aattacgttg
      601 gaaatctcat caaacactaa acaaactatc gatctgagtt ttccaatcga tccagcactt
      661 taccacagcc aggcaaacaa gctgatcgtc gataataaag aatatgacat tattgatgag
      721 acagaaaata agaaagatac agatgtgtca gtaccaaagc cagacgaaat agaagaagaa
      781 tcatcaaaag aaaacgaaaa ttctgtcagc ccatttacat tgcctacatt atccttgcca
      841 gctgtgagtg tgccatctaa tcaaacgatt cctacagaat atacaacaga tgatcagggc
      901 acttatccta aagccagttg gcaacctaca ggaaatacaa atgttcttga tcatcaaggc
      961 aataaaaacg gaacaaatca atgggatggt ataaattctt ggaatggaga tcctaatgat
     1021 cggacccatt cgtatatcga atatggagga accggtaatc aagcagacta tgcgatacga
     1081 aagtatgcaa aggaaacaag tacacccgga ttgtttgatg tttatttgaa tgctcgtgga
     1141 aatgtacaaa aagatatcac gcctcttgat ctcgtattgg tcgtagactg gtcaggaagt
     1201 atgaacgaca ataatcggat cggtgaagta aagattggtg tcgatcgttt tgtcgatact
     1261 ttagcagata gcggtatcac agacaaaatc aatatgggat atgtcggcta ctcaagcgaa
     1321 ggatatagct acagtaacgg tgcagtacag atgggttcat ttgattcagt gaaaaatcaa
     1381 gtaaaatcca ttacaccttc acggacaaat ggtggtactt ttacacaaaa agcactaaga
     1441 gatgcaggaa gcatgctatc cgttccaaat ggacataaaa aagtgatcgt tttgctgacg
     1501 gatggtgtac caacattttc ctataaagta cagcgggtac acgcacaatc aagcagcaat
     1561 tattacggaa ctcagttttc taatacgcaa gatcggccgg gaaatacttc tctaatctca
     1621 agaatctatg atgcacctga ccaaaacaat ctatccagaa gaatcgacag tacgtttatc
     1681 gcaaccatcg gagaagcgat ggcactcaaa gaacgaggaa tcgaaataca tggtcttggc
     1741 atccaacttc aaagcgatcc ggcagctggt ctctcaaaag cagaagtaga gtctcgtatg
     1801 cgacaaatgg tttcatcaga tgaaaaaggc gatctttact atgaatcagc tgatcatgca
     1861 acagatatct ctgaatacct agccaaaaaa gctgtacaga tctcagcaac tgtaagcaat
     1921 ggacaaataa atgatccaat cgcagaacca ttcatttatc agcctggtac actttcagtc
     1981 aagagtgtgg ggacaagtcc tacaacggtc actccatcta tttccataga aggaaatacc
     2041 atcaagagca atcagatcta tttaggaaaa gaccaagaaa tccaaatcca ttaccaagtg
     2101 agaatccaaa cagaaaatga ggacttccat ccaaatttct ggtatcaaat gaacggcagg
     2161 acaactttcc agccaaacat tgataccaat gaattagctg aattcggtat accatctgct
     2221 aaagctcccg gagtcagtct tcacatcaaa aagttatggg aagaatttga caacaatcta
     2281 gctgatcgtc cagatcaagt tacttttgag attcaacggg aacatacgac aaatgctgca
     2341 gcttggaaaa acggatatat tcgaatcatt aaaccagcta aagatacaac aaatacgtgg
     2401 gaacgtgcag acattgacaa attatctgca aatagcggag aaagttatca agagatatta
     2461 tcactacctc aatacaataa tcaaggtcaa gcattcagtt accaaacaat caaagaatta
     2521 cctgtaccag gatacgattc tcaacaaata gatgcaatga catggaaaaa tactaaacaa
     2581 ttcacaccgt taaacttgaa aataacgaaa aattcctcta caggtgaaaa ggatcttatt
     2641 ggcgctgttt tcaaattaac aggagattct attgatactt tactaacaga tcatggcgac
     2701 ggaacctatt ctcttccaga aaatgtcaaa ttgcaaaaag aaatgaccta tacgctgaca
     2761 gaaacaaaag ctccagaagg gcatggatta agcaaaaaga ctacttggga aatcaagatc
     2821 gcttctgatg gtacggtaac cattgatgga aaaacagtca ctacttccga tgatacgatc
     2881 cagttgacta ttgaaaatcc ttttgttgaa gttcctgtag cagtacgtaa gtatgcgatg
     2941 caagggacgg acaaagagat aaatcttaaa ggagcagcat tttccctaca gaaaaaagaa
     3001 gcaaatggta cttatcagcc aattgacagc caaacaacga atgaaaaagg tcttgccagt
     3061 tttgattcac tcacacctgg taaatatcga gtcgttgaaa cagctggtcc tgccggatat
     3121 gatacttcgc cgggaaatta tgaattccaa atcgataaat atggaaaaat catttacacg
     3181 ggaaaaaata ccgagatgac aaataatgta tggacgctca ctcatcaaaa tcgactaaaa
     3241 gcgtttgatc taacggtaca caaaaaagaa gacaacggac agacattaaa aggagcaaaa
     3301 ttcagactgc agggaccaga aatggactta gaatcgccaa aagatggaca agaaacagat
     3361 acctttctat tcgaaaattt aaaacctgga acttatacgc tgaccgaaac ttttacacca
     3421 gaaggatacc aaggtctaaa agagccagtt actatagtta tacacgaaga tgggtcaatt
     3481 caagtggatg gacaagatca tgaatctgtt ctgtcaccag gagccaaaaa caaccagatt
     3541 tctttagaca tcacgaatca ggcaaaagta ccattacctg aaacgggagg aattggccgt
     3601 ttaggaatct atctagtagg gatgattggt tgtgcgtttt ctatttggta tctttttttg
     3661 aaaaaagaaa gagggggcag ctaaatatga aaaaacttgg ttggcttagt atgtgtctct
     3721 tcttgttact atttaaacca gcttttactc aggtagcaac agaaacagaa acagaaatgg
     3781 ttcagattac tttacacaaa ttgcttttcc caaacgggca actgccgaaa aatcatccaa
     3841 atgacggaca agaaaaagct ttattacaaa cgtatcgagg attaaatggt gtcacattcc
     3901 aagtttatga tgtcacagat tctttttacc atctacggga aaagggcaaa acggtagaag
     3961 aagcacaagc agagatcgca aaaaacggtg cgtcttccgg tatgtttacc gcagaagcaa
     4021 caactacaac tcttaacaac gaagatggta tcgcttcttt ttctctggcc gctaaagatc
     4081 aagaaaaaag agataaagcg tatcttttca ttgaatccaa agtaccagaa gtcgtcaaag
     4141 aaaaggcaga gaatatggta gttgttcttc ctgtacatgg acaaaacaat caaaaacttt
     4201 caactatcca tttgtatcct aaaaatgaag aaaacgacta ccctgatcca ccttttgaga
     4261 aggtattaga agagcctaga aatgatttta cgattggtga aaaaatcact tattccttgc
     4321 atacgacaat tcctgtaaat atccttgact atcaaaagtt cgaattgtca gatagtgcgg
     4381 atgaagcatt aacgttttta cctaatagtt taacgatttc atcgaatgga gaaaagctga
     4441 cagaaggctt tgtcatacac aagaaacctc acggatttga tgttttattt tcgatccctt
     4501 cgttggaaaa atatgctgga aaaaaactga ccatttctta tcagatgcag ctaagcagta
     4561 cagcacaggc gaacaaggaa atcaacaaca acggaacact ggattttggt tttggtgtca
     4621 gtacaaagaa agtctctgta tatacaggga gtaagcaatt tgtcaaaatc gagacaaata
     4681 aaccagataa acgattagct ggcgcagtat tccttattaa aaacaaagca ggaaattacc
     4741 tccagcaaac agccaacgga tacaagtgga caaagaacga atcagatgcg cttcacctga
     4801 tttccgataa aaatggcgct ttttcaattt ccgggttgaa aacaggaagt tatcgattaa
     4861 aagagatcga agcaccttct ggttatattt taagtgaaac agaaattccg tttaccattt
     4921 caacttttct ttctgaggat aaagaggcgg acagtatatt gaaagtagtc aataaaaaag
     4981 aaaatagccg tccatttctt ccaaaaacaa acgaaacgaa aaatacactt ttaggcgttg
     5041 ttggtatggt attcgcaagc tttgcaatct ggttgtttat caaaaaaaga acaggagtga
     5101 aaaaatgaaa aatcataaaa aaataaacgt tatgttagga gtccttttcc ttattttacc
     5161 attactcaca aacagcttcg gcgcaaaaaa agtgtttgca gaggagacag cagctcaagt
     5221 catccttcat aaaaagaaaa tgactgattt acccgatcct ttaatccaaa acagcgggaa
     5281 agaaatgagc gaattcgatc aataccaagg attagccgat atttcatttt cagtttataa
     5341 cgtcactcaa gaattttatg cgcaacgaga taaaggagcg tccgtggatg cagcaaaaca
     5401 agcagtccag tctttgactc ctggtacacc agttgcttca ggaacgacag atgctgatgg
     5461 aaatgtcact ttatctttac ctaaaaaaca aaatgggaaa gatgcagtct acacgatcaa
     5521 agaagaacca aaagacggag tgtcagctgc cgcaaacatg gttttagctt tccctgtata
     5581 tgagatgatc aaacaagcag atggctctta taaatacggg acagaagaac tagatactat
     5641 ccatctctac cctaaaaata cagtctgtaa tgatggaacg ttgaaagtta caaaaatcgg
     5701 tactgccgaa aacgaagcac taaatggagc agaatttatt atttctaaag aagaaggaac
     5761 accaagcgtc aaaaaataca tccaaagtgt cacagatgga ttgtacactt ggacaactga
     5821 tcaaaccaaa gccaaacatt tcattactgg tcattcttat gacatcggca acaatgactt
     5881 tgccgaggca tctattgaaa aaggccagtt gatcgttaat catttagaag ttggaaaata
     5941 taatttagaa gaagtaaaag ctcctgataa tgcggaaatg attgaaaagc aaacaatcac
     6001 gccttttgag atcctggcaa atagccaaac accagtagaa aagaccatca aaaatgatac
     6061 gtctaaagtt gataaaacaa cacctcaatt gaatggaaaa gatgtcgcaa tcggtgaaaa
     6121 aattcaatat gagatttctg tcaatatccc attaggtatc gctgataaag aaggaacgca
     6181 aaacaagtac acaacattca aacttatcga tactcatgac gctgctttaa catttgataa
     6241 tgattcttca ggaacgtatg cttatgcctt atatgatgga aataaagaaa tcgacccagt
     6301 aaattattct gtcactgagc aaacagacgg attcacggtt tcagttgatc cgaattatat
     6361 tccttcatta actcctggcg gtacattgaa attcgtttac tatatgcatt tgaacgaaaa
     6421 agcagatcca accaaaggat tttctaacca agcaaatgtc gataacgggc atacaaatga
     6481 tcaaacacca ccgtcagtcg atgtcgttac tgggggcaaa cgatttgtta aagtagatgg
     6541 tgacgttaca tcagaccaaa cacttgctgg agcagaattc gtcgttcgtg atcaagatag
     6601 tgacacagcg aaatatttat cgatcgaccc atccacaaaa gccgtcagct gggtatcggc
     6661 gaaagaatca gcaacggttt ttacaaccac aagtaacggt ttaatcgatg tgacaggtct
     6721 aaaatatggc acgtactatc tggaagaaac gaaagcgcca gaaaaatatg ttccattaac
     6781 aaaccgtgta gcatttacta tcgatgaaca atcttatgta acagcaggac agttgatttc
     6841 tcctgaaaaa ataccaaata aacacaaagg tacacttcct tcaacaggcg gtaagggaat
     6901 ctatgtgtat atcggtgcag gagtagtcct tctactgatt gctggactgt actttgctag
     6961 acgcaagcac agtcagattt agtctttttg caagaattgg aaaaaggagc agcggattac
     7021 gcaaatgctc ctttttcttt tattatcaaa cagtcatcac aaggagaaca caaatgaaac
     7081 caaagaaaag gaaacttcta gatctgctga tgcttttctt gcttctctca ggaataggga
     7141 tactagctta tccatttgtt agcgacgcgc tgaataatta tctggatcag cagatcatct
     7201 ctcattatca gcaacaagcg gtcaaagaaa atgaagaagt catggctaaa atccaagaaa
     7261 acatgacaaa gaaaaaccag caattagcaa aagaaggtgg aaatccaggt gcagatccct
     7321 ttactaagaa aaaagaacct gcgaaaaagg atagaaccta ttttgagaaa catacgatcg
     7381 gtattttgac gatccctgag atcaatgtcc atttgccaat ttttgatgaa acgaatactg
     7441 tgctgctaga aaaaggtgcc tcattattgg aaggaacgtc ttatcctacg gggggagaaa
     7501 atacacatgc cgttatttcc agccatcgag gactgcctca agcaaagttg ttcacagact
     7561 taccgaaact gactttaggt gattctttct atatcgaaat caatggacgg acactcgctt
     7621 atcaagtcga tcagatcaag acggtagaac cgactgatac aaaagatctg agaatcgaaa
     7681 aagggcagga tttcgtgaca ctcctaactt gtacacctta tatggtcaac agtcatcgct
     7741 tacttgtcag agggcaccgg gttccttatc atgcaaaaga agttcaaaaa gaagtacaga
     7801 aagtatccga gaacaaacga tttttcctgt atggattgtt agctgcatgt atactgattt
     7861 tcctccttct tatatatctt tttcgtcaat cgttcaaaat aagaaacaga agttagctat
     7921 tcctcatttc tataatatat ctattcgaat cgttccgtaa acaaacaggc tgaggtttga
     7981 aaaagaaaac ttcagcctgt ttttatgatg atatcaaaga gagcctatca aacactatct
     8041 ttcttattac taaatgagga tcttttctta agttttccga tattctttaa gaaggccttt
     8101 ttgcatcaat tcttgtttgt ttttcaagtt gcggatattc tcttcaaaat tttgagaata
     8161 tatcactgag aataacgtat ttaatacgta atcgaaggca atttgagagg agaaagtcgc
     8221 tactttagca aaatcatatt cctcctgcga tataacaaga cacatatcag cctgtttggc
     8281 catttctgaa tgctgatttc cggtaatcag caaggtaggg gcctctacat gcttcaaata
     8341 ggtcattatt cgttcataat gatggatgcg accgctgtag ctaataaaaa tggcgcaatc
     8401 ctcgctagtc aaattggctg cattccaaga agaatcactg tattcttcag ccatgatcaa
     8461 aaacttattc aacttgacca ttttattctg gaatttcctt gccgtgattt gagaatcacc
     8521 ttttgcaaaa agaaagattc gctgtgcctt gttcaatact ttaccgattt gatctaaatc
     8581 ttcatgttca atctgcaatt gtgctttttt tattgactgg atcgataaat ccgccatttt
     8641 ctttgcaatt tgctgagtgc tatcttcact tgaaaaagga aaattaggat caatcgtttg
     8701 attgaactga gcaatcaatt gatgaacttc ccgccccagc tcgaaacgga attctttaaa
     8761 tccgctgaat cccatttttt tgcataaacg aatgatagct gaatgggaag tatacgtagc
     8821 ttttgctaat tcttcaatcg tcatgtagac tgcagcttcg atattcctac ggatgtaatc
     8881 gacaatccgt ttctctgtcg tggtaaacaa ctcttgtttt gttagttttt cttcaataag
     8941 catgatctga cctccaaatt ctatcttact gataaacact tggttccgct actatctttt
     9001 tgatttttat ccttactttg aacattaatt tcaccaaacc gaatgataac aagtgttttt
     9061 ctgaactttt ttaccagaat agctattata aatactttct gcgcttttct aggatatact
     9121 gaaaacatca ataaaaggag gcaccactca tgcttacaat cgcttatctt ggcaatggaa
     9181 agagtacaaa tcgctatcat ttgccttttt caagaaaact tacgaacaaa atcaatatca
     9241 aaaaaatttt ttccccatct gaaccagtgt ggaaaacatt cgaagacatc gaatatactg
     9301 atcagctgtc tgatctttgg gatgatcctg agatccaatt agttgtcatc actacaccta
     9361 gtcaatttca ttatgaatat ggaaaaatgg cactcgaaca tggtaagaat gtactagtag
     9421 agaaaccttt tgcagaaacg tcaaaggaag cagaagagtt atttgcatta gcaaaagaaa
     9481 aaggtctatt tatccaatgt taccaaaaca gacggttcga ttctgacttc ttgacagtcc
     9541 aaaaagtaat cgaaagcgga aaattagggg atattttaga agtagaaatg cattatgatt
     9601 atttcagacc ggaagtacca gaaaatacgc atgaattttc tactgcgaac agctatttat
     9661 atggacatgc ttgtcataca gtagatcaag ttctttctta tttcggcgaa ccagatgata
     9721 tcagctatga tgtacgtcaa cttttaggga aaggacgaat gaatgattac ttcgatctgg
     9781 attttttcta tggaaatatg aaaatttcga taaaatccag ttatttccgc atcaaagaac
     9841 gtccaagttt cgtcgtttac ggaaaaaaag ggatgttcgt gaaacaaaca aaagatcgac
     9901 aagaagaaca tctgaaaatg ttttacatgc ctgatcatga agactttggt atcgatttgc
     9961 ccgaacatta tggtgtattg acctatatag atgaacaagg gagttatcat gaagaaaaag
    10021 taatttccga ggttggtgat tacagccgtg tctacgaagg gatttacgag acattagtta
    10081 atggcgcaga aaaagtcatc aaagatgaag aaacaatctt acaaatgaaa attttggaag
    10141 aaggaatcaa aagcattcaa gaatcagaag gaaaaatatg acatatttgt aagaatcggg
    10201 tttgtttttt gacgcataaa ctgtcttatt gtacgatgaa aaagtataga actactagga
    10261 ggaaccaaca tgacaaatta tgacggtgaa aaacacaaac gattaagagg ttcaatggcc
    10321 cctaaaacag aagaagaagt ggctttagca ggaactaatt ctggtgaaac agaagacaaa
    10381 gaagaatcat tagctggcac aaactctggt gaaacagaaa ataaagaaac agctttatct
    10441 ggttcaaatt ctgtagaaga ggaaacaaag cctaatgagc ccaatcactt tgaaaaagat
    10501 atcgaaaaag cggttgaaga tgtcaaagaa aaatttgaag aactgacctc aaaaaataaa
    10561 catgatcatc attgatcgga cgaaatcaat tcataaaagc aggagacatt tggtcacctg
    10621 ctttttttct gtatccattc gatagaaatc gttcggtaca gtcgtaaaaa aaatccaact
    10681 gtaagttgta ccaaacaccc ctcgatttaa aagcgctacc atactagaat ataagtagga
    10741 ggtgaacagc gctgtgaact acaaaattag ttatctcctg aaaacaccaa gagaaatcag
    10801 tctagaaaaa gcagcagaga tcatcgaagt aaatacaagc gaggcgatca aacaattaac
    10861 tccttttgcc gaatatgtgg atgatacccg tatccgtata aagaaattcc gagcaatcga
    10921 ctgggcagaa aaatttttgg atattactcg atttgaagct gtgtataccg aacaagagcg
    10981 aagagatctt ctttatttga tggtttattc gactgcaacg tctgtttctg tttttcattt
    11041 tcaagatttt cttcaggttt ctaaagggac agtcctgtca gatatcaaaa agataagaaa
    11101 tgaattagaa gtggaagatt gtcagctgtc gtatactagg aaaactggtt ttaggctgct
    11161 agggtcagaa gcaatattaa gaagagaagc aaaaaatcgc actgctcgtt tgatgcagtc
    11221 acttgctgga agatttgggc tcacgtattt aaccgcaacc ctttccattg attattacgc
    11281 caaagttaga gatttgctag ataaaacgat ttcactatca gatcttcgat tcgcaccaaa
    11341 tcgaatggat gaattagctt attatttgag tttgacaacc attcgaattg gaaaaacatc
    11401 catggaactg gtaaaagatg ctgaactgtt ggcttctctt gcagtcactg acattagtcg
    11461 acaagtattg actggccttg gctattccga acatcaaaat tcggagatta tatttttctc
    11521 tctttgctta gcatcgatcg ttgaaggaga tgtccaggag cctgcacttg attttttgct
    11581 ggaatgttct tcagaagtca tccatcgcat ggaattcctg atggccgtag aatttgcttc
    11641 gtttcgtgaa ttattgatga atgtctttta tcacttggtc cctgcctatt ttcgtatttc
    11701 ctattctttt tatttaccta atgtgatgat cgatcaaatt aagcatcaat atgcttccat
    11761 ctacgaaatg acaagaaaag cattacttcc gttagaaaga cgaataggga agagcatccc
    11821 tgaagaagag attggttttt tcactatact atttggcgga gagatcagaa aagcggacgc
    11881 agaagaaaga aatagaaaaa taagagccgt gatcgtctgt ccaagtggca ttagctcttc
    11941 attgatctta aaaagtgaac ttcagcaatt attcccgatg atctttttta cagagactaa
    12001 ttcagctgac cgcttacacg aggttgacga aacttcttac gatattattt tttctactgt
    12061 acctctcaca cttactgata agaaaaagtc tctctatgtt ctgcatccaa tcatgactga
    12121 cttggaaaaa aatcagctga tcaatcaagt acaaagagat tggctgatac caggattttc
    12181 aatgccttct gcccaagaat tgttgtcggc gctactgcct tatatcgaat taaaagaagg
    12241 cgtagacgaa gaaaagctct atcaagtatt aaatcgtaaa atgaacaaac ttttagaaaa
    12301 gaaggaggat caacgtccta tgctatctga attattaaca gaagaaatga tccaattatc
    12361 ggatcaacca aaaaactggc aagaagcaat cacatttgcc gcacagccgc tattatcaga
    12421 aaataaaata gaagaaagtt atatcaaagc tatgatcgag cgcgtggagc agtatggtgc
    12481 gtttatccat attggcgatc acatcgcatt acctcatgct cgtccagaag atggcgtaaa
    12541 cgaagtaggg atgtctttgc tgaaacttca acaacctgtg gatctgcttg ataatccagc
    12601 ccatcccatt agtttgttca tctgtttagc tgctatcgat aatgaggcac atcttcgggc
    12661 tttggcaaat ttaacaaagc tactttctaa taaacaaaat cttcaagatt tattacacgc
    12721 atcaacaaaa gaagaaatat tagcgatcat aagaaaagga gacgaatgat atgaaaattt
    12781 ttgcagtatg tcaaagtgga ttaggaacaa gttttatggt agagatgaac atcaaacaag
    12841 cacttgcaga tcttggagca gatgtggacg agtttgaagt aagtcatacg gatgttggaa
    12901 gtgtatctag cgatatggct gattatttct tcattgagca ttctttgaaa gatacgctaa
    12961 cttctatacc tgatgaaaag ctcgtacctt tgcaatcgat cattgacagc gaagaagtaa
    13021 aagaaaaagt aacgaagatc ttgtcaaaag aataaaaatg attcaaggga ggaactatca
    13081 atggataaat tgttgaattt gtttgtgtca atcgtcagtc agccctccat cctcgtttct
    13141 ttgattgcac tgcttggtct cgttcttcag aagaagaaat tttcagaagt gatccaagga
    13201 actatcaaaa catttgtcgg attcctagta ctgatcggcg gtgctggttt aatcagtaat
    13261 tcactaaccc cttttgcttc gatgttccaa acagctctgc atactcaagg agtagttcct
    13321 agcaatgaag cagtagtggc gattgctttg caacaatacg gtacacctac tgctttgatc
    13381 atgctagtag ggatgatcgt gaatatttta cttgctagat tcacaagata taaattcatt
    13441 tttcttacag gtcaagcaat gctttatgtt tcttgtctaa cagcagtcat tttagtaagc
    13501 gcggggttta ctgcaaattt gtggatgatc ttattaggcg gggtcttcga aggaacacta
    13561 ttaactatta cgcctgctct ttgtcaaccg tttatgaaga acatcactgg aaacaatcaa
    13621 gttgcgttag ggcataccgg aaatatcggt tatgcttcta gtggatggat cggaaagttt
    13681 tttggaaaca aagaccgttc tacagaggac ataaagattc ctaaaagttt cagctttttg
    13741 cgagattcta ctgtatcgat catgctcttg atgtcgatcg tatatgtcgc acttgctttg
    13801 ttagctggtc ctggatttgt tgaaagagaa ctaagcaatg gagacaacgc tgtaatcttt
    13861 gcactgatcc aagctggaac attcacagct gggtttgtta tcgtccttca aggggttcga
    13921 atggtgttgg cagaaatcgt tccagcattc caagggattg ctaaaaaact cgtacccgat
    13981 tctaaaccag cattagatgt tcctatcgtt tttccatatg cacctaatgc tgtattaatt
    14041 ggcttttttg ttagcttctt tgtaggaaca ctatccatgt ttgctatgat aggcttgcaa
    14101 actacagtta tcattcctgg ggtagtagga catttcttct gcggagctgc ttctggtgtc
    14161 ttcggcaact cgactggcgg tagaagaggg gcggtattag gttctgcttt caatagctta
    14221 ttgattagtt ggctgccatt atttatcctt ccagtattag gagatcttaa aatcgcttct
    14281 tctacatttg ccgatactga ttatctagta ccaggacttg tacttgggaa actaggagaa
    14341 tacggtcaaa tagccttgac agttggaatc atcgctttct ttttacttgt cgtattatct
    14401 agctttcttt ttgccggaaa aagaacaaac attgtttcta aagaacagaa ttaagcaaac
    14461 ttcaaaataa aaattgcttg gacaatatca gaagaagatt taaagtcttc tgatatgtcg
    14521 caagcagttt tttgttttat gcatttattt ttgaatagct tgttaagaca agtggctgtt
    14581 ttgctagaag aggataactt ttaccattca ttttcactgt tccgcctgtt ctatcagaag
    14641 taatcacgat cttatctttt gttttttcaa gcgaaatctt tccatctttg ataggtagcg
    14701 tacaattcag tgtttcaaat gaatctagat taggttgtat cataaattct tcataacctg
    14761 gctttgttgg atacaaaccg ataaaatagc gtcctaaaag atagattggg cttgctcccc
    14821 atgcatggca taagcttttt ccataaggat ctccatacat actgtacttt tcatttcctg
    14881 tttgagtagg gtcgtattct tcccagaaag ttaccgcatc atgagataac atccctcccc
    14941 aataatcaag aatcgtttgg tagacgatat tcgtctgtcc gagtttgcat aaagcatctt
    15001 gttcatagaa tttgaaatat ggagtagtaa tctgtgtgat agtgtcattc aataagacat
    15061 tctttaagat cgattgctgt tggtctttgt tagcaaaatc gaataaaaca gcgaaaatat
    15121 tcgcatgacg cgtcacattt cttctcccag attcatagca atcgataaaa gcgccaagtt
    15181 cttcatccca aaagtagtca aataggtttt gtttcaatag ttgatatttt tcttgataga
    15241 gatgagtatg ataattttcg ttctctccat atgtttcagc taagatatct ccgcaagtga
    15301 tcatggaatg ataggctttc agtaataaga cttgttctgc agcaagcgtt ccttctttat
    15361 ccatttcaga ccaatcaata aatatccaat cttttgctct cccataaata aaccctaatt
    15421 cattcgtttg ctccatcaag tatctcatta atgattgcat cttagggtag acgatgcgta
    15481 aaaattcata gtctttcgtc atcatatagt ggttttcgat accaatcaac cacaagatcg
    15541 aatagtcaac gatagtattc atatgctgct tgatttcatt ttgtcctctt aatgctaata
    15601 tcgttcgttt attgatttcc tcgtcaaaaa aaagatattg attgatcatg aatgattgat
    15661 acgcatctcc agaccaaatc cagcgatctc gtttgatacc gtcgataaaa aacaaatcac
    15721 tacataactt gaaggtttct tctgatactg accagatctg attcagtaaa gaagaatcgc
    15781 ttgaaaaagc agcttgtttc gaaagagata aaaattcatg aaaaacttga agttgaacca
    15841 ttccagtaga aacctctggt ataaacaaat agcgaaaagc gcgtttacga gctttcattt
    15901 ttcttgtcat tcctatttgc ttatagtagc aataatcgat atccatagct tctacatcgg
    15961 actctccata acatagagtg atagattcga tatggtcatc cagagatatt agttctacac
    16021 agccattcac tgctcgccca aaatcgaata agattcctcc gccaatctcc gtttcagtga
    16081 ctggatggac gcattctttt tgataataga tttgatttgg atcttttgcg atatcgacat
    16141 aaagcggact ccagccagct ggatattctt caataaaatt acttgctgtc cagccaatat
    16201 ctgattttac ttcgtctccc tcgataaaca tcgctggtag ccctgatgta tgtcctacaa
    16261 agatacgaat ttttgcttta ccagctggac aagtcagcca ttttccacat ggatgttttt
    16321 gtccattaat ttctacatac ccaactcctt ggatcgtcac tttaaacatg gaaggttgat
    16381 caagaaagta atatctctta aacttgacgt tccgatggca atcatccatg taccagtacg
    16441 ctggccagtc aaaacctcgt tcttcccgtt ggaaattttg tagcagtcca tggcggatct
    16501 caaagtctcc aggataccaa agccatctat ctttatgttt ttcatttgtc attttacacg
    16561 acctccatca aatttatttt gttgtcaatt gttcttttat ttcttcatct actcttatca
    16621 ctgtctttct attcgtcctg tgtactacta gaaggctaat aagcgcgcta atcaacagca
    16681 agatactgcc attgacgaaa acagcaagtg ttttactccc aagagaagca aaagagagaa
    16741 agagcaaacc atatggtgca atgaaagtac ctaaattagc gccaaccaat aagattgaag
    16801 tattgaaact ctccgcgtac ttgggcgctt ggatacttac ttggttgaat aagtaaggaa
    16861 tgaataacga aaaagaaatt cctgttagga tcgcaccaaa cccaatcaca tacaggttta
    16921 tcacagtggc aatcaataca aagccgattc ccatcagtag taaagaaagt gtaagcatat
    16981 agcgatgtgc ttttgtctga agccagccaa aacaaactcc ggcagccata cttgccaatt
    17041 gcatgattgt taagatattc gctgcattag tttcgtttcc tagagaataa gtcgtcagga
    17101 ctgtggctaa tttaatcgat acagcattat ataccacgat gaccagcaag agaaaaatac
    17161 cataactaat cattttagga ttcgttttga ttacttgtct tgttggggct gtgccatgtg
    17221 attgaagttc agttgtttca ggtgtaggaa caaacaaaac aaacaagaca agaataggta
    17281 agctgatggc gtagactaaa aaggtatatt gccaaccgaa ctgtactagc tgtcctgcaa
    17341 caaatgtcat taaagctgca ccaaatcctt gaaatgcact ttgaaaacca atcatagagg
    17401 ctcgttcatc tccgtgataa aaggcagcaa tcatgctgac tgccaaagaa ttgatcaatc
    17461 caaagccaat acctagtcca gctcttgaag ctaagacaat actgtaactt tgtgagaaga
    17521 gggggaggat accataaatc gatgcaatca ctaatccgat gatcacagtt tgtttttgtc
    17581 cgattttcct agcaataaaa ctactaatca aaacaaaaat agtgaccata aaagagggag
    17641 cagtcgttaa taattcaaca gcagataaag aacgcgtggc ataagtagct gacatactgg
    17701 gaattgcaga cgtgactgca ccagcagaag tcaaaataag agagactgac agcagagaaa
    17761 atttcatcat ctttttattc ataagcatgt tcctttcatt tttgtaagcg cttatcaaaa
    17821 atatagtatg aagtggttat tctctcaata gcggaatcac aggaagttca tgtcccaacc
    17881 atagaaagtc tttatgaaac caaaaatagc tttgcatata atagcagtac agcgtgaact
    17941 aataagcgaa agaaaccgct tacaaaaagg agggagtcaa ttggaactag ctgaattaga
    18001 catttttttt gcgtaacgaa gaatcaatcg aaactctgca gcgtgaacaa ggaatgaata
    18061 tcaatgattt aaacttagct taccatcttc agacaccgcc aaaaatccac cgaggggatc
    18121 tatttcaaca gggaaatatt catatcagta aacaccgtcg atatgcggaa gttcccgagc
    18181 atacacatga ttttgttgaa atcaattata tgtatagcgg tacatgttta caaattatta
    18241 atggagaaag gatagagatg gaagaaagtg acgtggtgat cattgataaa gaagctactc
    18301 aaagaatcga atacacaggg caagaggata ttttaatcaa catcttatta aaagaagaaa
    18361 ccttatctac agagatattg aatcatttgg caagcacaaa taatctactt tcttcttttt
    18421 tgataaatgc atcaaaagaa aaaggggaac atacacaatt cattcatttt cgcggcggaa
    18481 aaacaagtcg tttgacgcat ttgattgaaa atatgctgtt gatttatttt gatacataca
    18541 gtcataagat ccgttcgtta aatctctata catcactatt actgatcgaa ttgactcatt
    18601 tattagaaca acaaagcttt tcatcagcag atggatgcgc agaaaatgag atgcttagag
    18661 tattagatta tatcgataag cattttactt cttgtacgtt aaaagaaaca gcagctcatt
    18721 ttggctacaa tccggtctat cttagtaata aattgaaaat gtattttgat gcttcctttc
    18781 aagaactggt tctaaagaaa aaattcgact ttgcgttgga actgattcaa gaaacgagtt
    18841 tttcgttgga agaaatcgct aatcgtatcg gatataaaca aacgacttct ttattcaaat
    18901 tattcaagaa gtattcaaca tgtactccca atgaatggag aaataaacat taagaagaaa
    18961 atctttcact aaagcatatg aaggaaaatt gttttaggtg cgattgctgt cttattatat
    19021 cgttgcagcg aatttttttg gatttatttt cagttctaaa cttttggttg tattatcagg
    19081 ggatataggt gtaaacattt gtcatttatc tgttatgcgt attcaggaat tcgtttccat
    19141 caaaaattat aataacaact attataatta acatgttgat aaaatgaata aacaagacta
    19201 tacttttcat gaaagtaaca aatagttatg aaaaggagct gtttattcat gaaaaaaaca
    19261 agtcgtaaag tagtaatcgt cggtacaggt tttgttggaa caagtatcgc ttatgcaatg
    19321 atcaaccaag gaatctctaa tgaattagta ttgatcgatg tgaatcaaga aaaagcagaa
    19381 ggggaagcat tggacctttt agatggtatg gcttggggcg atgaaaatgt ggctgtatgg
    19441 tcaggtggct atgaagaatg taaagatgct gatatcgttg ttatcacagc tggtatcaac
    19501 caaaaacctg gtcaatctcg tttagattta gttaaaacaa atgcgtcaat catgcgccaa
    19561 atcgtaaaag aaatcatggg atcaggattc gacggtatta tcgttgttgc ttcaaaccca
    19621 gtagatattt tgacatatat cgcttggaac gaatctggtc tgccaacatc acgtgttatc
    19681 ggtactggaa caacattgga tacaactcgt ttccgtaaag aaatcgcatt gaaattaaaa
    19741 gttgacccac gcagcgtcca cggctatatc ttaggtgaac atggtgattc agaagttgct
    19801 gcatggtcac atacaactgt cggtggtaaa ccagtgtttg aaattgtaga aaaagaccat
    19861 cgtattgccc aagatgaatt agatgtgatt gctgataaag tccgtaatgc tgcttatgag
    19921 atcattgatc gtaaaaaagc aacttactat ggtattggta tgagtactgc acgtatcgta
    19981 aaagctatct tgaataacga acaagctgta ttgcctgtat ctgcttattt aactggtgaa
    20041 tacgacgaaa aagatatctt tacaggtgtg ccatcaatcg ttgacgaaaa tggtgttcgt
    20101 gaagttgttg aattatcaat caacgaagaa gaaaaagcaa tgttcagcaa atcaacaagt
    20161 gctttaagag aagtattgaa cactgtatta taagaaacaa tgaatatgaa ctaaaaatcc
    20221 taaaactgct taacagctga aatagctgtt aagcagtttt ttattgatat ctaataggta
    20281 agcaaataaa cagaatggtt aaaattaatc ggtccgaaaa tgtatccgta catatgaaca
    20341 tatgaaattt aatctgttga ttacaataag cgaatcgtta tcaatgttta tctgtggtta
    20401 attaaaaaat ccttccgcaa cggcaaatac aatgattcat atctaattcg ttcatatttg
    20461 attaactgaa taaagaaact ggtttttatc attcaattaa acgacaaaaa aagtaagaaa
    20521 aactgcaaaa tttttttata attttgcata agcagatttt gatggaaaaa tgacgatttt
    20581 tgataaaaaa ccgttcgaga gaggttta
//
LOCUS       DAFCLG010000039        20571 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFCLG010000039 DAFCLG010000000
VERSION     DAFCLG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.039"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(44..2101)
                     /locus_tag="LND56_001346"
     CDS             complement(44..2101)
                     /locus_tag="LND56_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBK6743279.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2215..2427)
                     /locus_tag="LND56_001347"
     CDS             complement(2215..2427)
                     /locus_tag="LND56_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743280.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            2614..3081
                     /locus_tag="LND56_001348"
     CDS             2614..3081
                     /locus_tag="LND56_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743281.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            3078..3320
                     /locus_tag="LND56_001349"
     CDS             3078..3320
                     /locus_tag="LND56_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBK6743282.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            3338..3640
                     /locus_tag="LND56_001350"
     CDS             3338..3640
                     /locus_tag="LND56_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743283.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            3712..5766
                     /locus_tag="LND56_001351"
     CDS             3712..5766
                     /locus_tag="LND56_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743284.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            5766..8378
                     /locus_tag="LND56_001352"
     CDS             5766..8378
                     /locus_tag="LND56_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBK6743285.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            8375..10753
                     /locus_tag="LND56_001353"
     CDS             8375..10753
                     /locus_tag="LND56_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743286.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            10814..11356
                     /locus_tag="LND56_001354"
     CDS             10814..11356
                     /locus_tag="LND56_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743287.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            11434..11766
                     /locus_tag="LND56_001355"
     CDS             11434..11766
                     /locus_tag="LND56_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743288.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11767..12360
                     /locus_tag="LND56_001356"
     CDS             11767..12360
                     /locus_tag="LND56_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743289.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12382..14076
                     /locus_tag="LND56_001357"
     CDS             12382..14076
                     /locus_tag="LND56_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK6743290.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            14764..16641
                     /gene="ltrA"
                     /locus_tag="LND56_001358"
     CDS             14764..16641
                     /gene="ltrA"
                     /locus_tag="LND56_001358"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6743291.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            16772..17149
                     /locus_tag="LND56_001359"
                     /pseudo
     CDS             16772..17149
                     /locus_tag="LND56_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            17149..18354
                     /locus_tag="LND56_001360"
     CDS             17149..18354
                     /locus_tag="LND56_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6743292.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            18370..19014
                     /locus_tag="LND56_001361"
     CDS             18370..19014
                     /locus_tag="LND56_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743293.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            19025..19831
                     /locus_tag="LND56_001362"
     CDS             19025..19831
                     /locus_tag="LND56_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743294.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            19853..20083
                     /locus_tag="LND56_001363"
     CDS             19853..20083
                     /locus_tag="LND56_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743295.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         7387 a         3249 c         4188 g         5747 t
ORIGIN      
        1 aagatttctc tttaccaaca tcatttgaca aagagccttt cttttatttg aagattggat
       61 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
      121 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
      181 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
      241 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
      301 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
      361 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
      421 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
      481 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
      541 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
      601 gatcagtcat gtttttccaa ggagtagcaa agctatcccc ttcgtatact gtttttgatt
      661 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
      721 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
      781 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
      841 tgtcaccgtg agtgacattg actatctgtt ctttcaaaat attaactcgt ccttcgtttt
      901 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
      961 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     1021 tatctttagc gccttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     1081 aagttacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     1141 tagtaaccgt atattctttt ggtgtttcag gtttgtgttg caacccttta aatgcagcat
     1201 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     1261 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     1321 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     1381 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     1441 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     1501 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     1561 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     1621 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     1681 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     1741 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     1801 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     1861 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     1921 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     1981 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     2041 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     2101 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     2161 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
     2221 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
     2281 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
     2341 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
     2401 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
     2461 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
     2521 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
     2581 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
     2641 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
     2701 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
     2761 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
     2821 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
     2881 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
     2941 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
     3001 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
     3061 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
     3121 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
     3181 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
     3241 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
     3301 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
     3361 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
     3421 cgcaactagt ttgaaaaata atctgatgat ttttgctgct gttgcggcag tggcttccat
     3481 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
     3541 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
     3601 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
     3661 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
     3721 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
     3781 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
     3841 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
     3901 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
     3961 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
     4021 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
     4081 cttttacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
     4141 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
     4201 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
     4261 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
     4321 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
     4381 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
     4441 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
     4501 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
     4561 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
     4621 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
     4681 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
     4741 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
     4801 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
     4861 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
     4921 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
     4981 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
     5041 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
     5101 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
     5161 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
     5221 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
     5281 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
     5341 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
     5401 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
     5461 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
     5521 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
     5581 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
     5641 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
     5701 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
     5761 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
     5821 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
     5881 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
     5941 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
     6001 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
     6061 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
     6121 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
     6181 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
     6241 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
     6301 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
     6361 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
     6421 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
     6481 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
     6541 tgagtgataa tccattggca ctcattgtga aatactggaa gcgaggagac atagacaccg
     6601 caacacagtt aaccaatact tttacgaata cgatttatta tgatgcaaat gcttcagaca
     6661 ataaatactt taacgaaaat gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg
     6721 aacaagcgga tcaaacaggg gattactcca aattaacacc aaataatctc gttgaactac
     6781 tttcagaaat tggcggtttt aattattatc cagatcctga gaatgaattc aagcagctca
     6841 atgccttaga tgagttcatg aatcagttac cccctggaaa tatagcaaaa aaacaatatg
     6901 gggcaacaaa aattggatct gataaagcga aagggaatat cttgtctacg gcaattacag
     6961 gattgaatcc atttacccta acgaaaattg cgaagatgac ttctcaatcg accattgatt
     7021 acaagtccat tggttttccg aagtatttgg atctcaaatt acatgaaagc ctattaaatc
     7081 aacgcattgt cgtggaattt tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag
     7141 ttggctatcg tggtttttgt gaaatcaact ttgattgtca tttagagcag ggagatcatg
     7201 tttgcttaaa atacaatcat caaggaaaac aatatgttgc ttggtatgag ttttctcaaa
     7261 aagtattgaa agacgaagag ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt
     7321 tgccagaact agaaaaagaa gccgttctaa cattagatga gaagaaaagt aacctattag
     7381 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg ttaacgcctg
     7441 attatgatcc atctaaccat atcattgtta gcatctttct tagtcagttg tataaagaat
     7501 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgtaggatt cattttattt
     7561 tagatgaatt tggaaatatg ccaccgatgg acaacatgga aggaatcatg acggttacgg
     7621 caggtcgtaa tatgttatgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
     7681 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
     7741 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
     7801 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
     7861 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
     7921 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
     7981 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
     8041 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
     8101 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
     8161 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
     8221 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca acggatcgtc
     8281 ttcaagaata tgttgaaaag ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac
     8341 ggctgttatt ggaagctgaa aaggcggtga aagaatgatc attggaggaa ttttctctgg
     8401 catagccgac tggtggtcag gtttattcgg agaaggggat gcagatccca aaaccgtaac
     8461 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattca gttggatttg
     8521 taatactttt ttatggggat tgatcaaagg gttatataat ctcaatactt tattagaaaa
     8581 aacaatttat caaagctttg atttaaaaga cttattgaac gccgctggtg ttaatgaact
     8641 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
     8701 aggcttgaaa ttttttatga gtaaacaccc accgaaagtc aaaaatatct tggttaattt
     8761 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
     8821 cagtaaaaac ttttatggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
     8881 tgcttttcaa attattcaaa ataatgtgat tgatgtcagt acattactag cgactgaccc
     8941 gaataaagta gaatccttac caacagataa aagaaatgct ttaacagcga aaaatttcaa
     9001 aacagcgaat atcaatggga ttattacacc tgatcaagct aagaaaatgt ctgaatcaag
     9061 tgatattagt acagtacaaa aagatcggtt aaaggcatta cagtatcgac tagagttaga
     9121 cgataatggg aaagaaatcc ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta
     9181 tacgtcaggc tatcgtcgat acattagtag tccagggatt attttggctg gggaatcttc
     9241 tttgtcggta gcctatttat ttattttgtt tacaatcgtt acttgcatta ttgagctagt
     9301 atttaaaaaa ttctatttag taattgccgc gtcaacggac tttgagacag gacaacggat
     9361 gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta cgattttaga
     9421 attacgaatc tatacattaa tgctttcagg tatcggtgat ttgcatgcgg taggaaagat
     9481 caatggattc ttgtatgttg ttgctttaat tgtgctaact attgcgctat tcaaaggctc
     9541 tcaatcggtt acgaagattt tcggtgttga tacgagtctg aaaaacggca gcaattctct
     9601 tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct
     9661 tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaga atggatttgg
     9721 ccatcgaaaa gatattaccc atgataacgg ggaagaacaa tcagacgata aagctacaac
     9781 tgtacctaat agtaagaaga cagtttccga aatgttcggg aaagcgaaaa atggtgtttc
     9841 taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga
     9901 tcttacagaa aaagccaaag acaccttaga agagaaatta gacggcacga gagctaaaga
     9961 atttgctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag aaaaagcttc
    10021 agcagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg cagcagtgaa
    10081 ggcaaataca acaagtgaaa aagaaaatca agaaggtgca gcgattcctt cagccaaaga
    10141 tcaccaagat gagttacatt ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg
    10201 acaacatggt cagttaccaa aaaccgatcc agaacaatat cgttataaat ctaacattaa
    10261 ttctgaaaac gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga
    10321 tgaggattcc atcgatctgc caaatgataa tggcatcgaa gttggaactg aaaaatcaac
    10381 gaaaattggt ttgcctaaga cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt
    10441 caaagagaat gatcaaagct ttgttcatca atcacctgca ggtactgttc aaatgaaatc
    10501 aagtcgtttg cctgaaaatg gaaatctgac aactgtttct tacaatttag ctgaaagtgg
    10561 tgaaacagag aatgcgtccg ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat
    10621 caataaagtt cataatggat acagaccagc aaacgtagca tttactgcac cgacaaaaat
    10681 aaatgtgaca agagaaacta aaaacaggaa caatgaattg aaagaacaac tattgaagga
    10741 tatcaaacca taaaaggcct atttaagggc ctttttcttt ataaaacaag tttaaaatgg
    10801 aaaggagaaa attgtggagg atattttaat agctacgttt ataactgcac tgggagcatt
    10861 tttaacgata tttgtggtga ctattaaagg atttaaagat acggatagtt tacaaaaaga
    10921 tcatcagtct attttagatg gtcaaaaaac acataccgaa agcattattg tacacctttc
    10981 agaaggattc acttatttga aagatgctat gcccacattc acagtaaaat taacggaaat
    11041 atttcattct gttgagcaaa tatcgtcaga attgaaagag caaagacaac aaaaagatcg
    11101 tcaacttggc tcacttaatt ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa
    11161 tcgattaaac gatgaaatgc aacgagttca tttggaaaat gaagaattac gcgcacaaaa
    11221 ttatgattta attcaaaaga aaagaaatct ggaacaaaaa attgttgaat taaaagaaga
    11281 aaatcgtcaa ttgaattatc aattacgacg aggaaaaaat cgttctcgtg atttaggaaa
    11341 tgaattagag ttgtaatatt tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct
    11401 aaaaatacaa aatataaggg gtatggttat attatggata aatacattca tatgaaaaat
    11461 cctccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga tctattaacg
    11521 cttggtggct gctttttttg ggcgaaaata gtcaataatc ttgtccactt accactcttt
    11581 tttgcactct tgaattatgg attatggctt gcgatagcgt tagtgattgt ttggcgaccc
    11641 tataataacc ctggtcaacg ccaaattttt gcgattatcg aatctatttt ctttacggat
    11701 aagaaccact atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    11761 gaataaatgc gagtcaaagg acaaaattca gttgataaga aatccatttt gcagtttgga
    11821 aaaccagaag agcgctctat cttggactta tttccatata aatcctgtac agaagaggga
    11881 gtcttaatta ctagagaaga tcattttcag cgttattatc gtgtcgtttc cactgacgtg
    11941 gagggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    12001 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    12061 atcgtgcaaa agcggcagtt attagataaa aatcgtatgg aacagtcact aggaagaaat
    12121 ctacagcgat taagaaaata tgaaaagaaa ttagtagagg agatccagga attaaaacgg
    12181 gcagaacaag aacgtcctga cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag
    12241 gatttacaaa ctagaaatcg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    12301 ctctacaaaa aagaattgat taagattctt tatcgaatga acaacatgaa tgacgaataa
    12361 aaaagatgga ggaaatgatt catgctagat gtgataaaaa cagctttttc aacgcaaagt
    12421 aatcaaaagg tgattgatcc attaaaacaa cttggtaatc gtgaaataga gattttaaaa
    12481 tcaaaaggtt atgatttaga tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt
    12541 gatgaagaat atgtgaccta tggaaatggt tattcacgat gtgtgacagt ttatcattac
    12601 acaaaaaatc ctactctttt ttggttggcc aatctcatga acaatcctca tacagtggcc
    12661 acactagatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta
    12721 gatgaattaa atgatcgatc agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg
    12781 gaagaatacc tggaactagc ggaatatgcc cgaaaattga cttctaatgg ggaaatttct
    12841 aaacaagtca aagtgagagt acatgtttac ggaaatacga tccaagaagt ggatgaacgg
    12901 gcgcaggaac tgatcgaaaa tctaaaaggg atggattatc gtgcggtgac ttatctgcat
    12961 aaaaccaaac gagattggca atcgatgttt accatgttcg atgaacaagc tgaatggttt
    13021 ggaaatatca aagggcaatc acttccatcg ttaaacattg gcggaggatt cccgttccac
    13081 caccaaacgc taaaagatcc aggaggcaca caatatgggg aaaccatgac aggcggccca
    13141 tttgtctgga atgccacgta taaaaatagt gtacgtttat ctaccaatac cttgcttcta
    13201 ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc ccatttagct
    13261 gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccttt cttaaaggaa
    13321 tataacggta ttatggtgcg tttagacgga tctgacggaa tgatcaatcc tttagagata
    13381 tttgccacac ggacgtatga tgaagcatcc agtttatatg acgatggatc agtcaaagat
    13441 ttgaagatca atgaagcagc gtcctatcaa acacacttag acaaagtggt ctatcaggta
    13501 cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac ctacttaaat
    13561 cgtttttatg aagagtatgg aacagtacca cgcggcttta cttcctcaaa ttcgcgttca
    13621 aatactgata cgcaagtcac agggaaagat ccagaagctt atccgacgtt caaagaattt
    13681 ttagcctttt taggacaact ggaactacca ggggcttccc aagagaaaaa gaatcggaaa
    13741 gaagaaatgg agtctatcgt ggaatcctta tgtgagactt atggcatgat ttttgatggt
    13801 catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat tgactctatt
    13861 acaggcctag atcgaggggt acaacaatgt cagatgtaca tggccatgac gttgatttgg
    13921 aatcaggcat tgattcaagg acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa
    13981 gaacaagact tcaaacgctt cttggcgatg attgatgagt gccataatgg tgcgacctgt
    14041 tctgcagtga aaagctgtgg gttgcaagta gtctaaaaac tattgtaata actcttataa
    14101 cgagaagtca aatgagtggg taacgccaca aagggcttcc actgatactc cgacatgcaa
    14161 caagtagttt atgcagacca acgttgtgtg aagctcggtg aagtcggctg aaggataccg
    14221 taaggtaaaa gaaatagtga ataccacgaa agttctctag aggtaaaggg tgtatccgat
    14281 aagtcgggtg tccaaaaaaa cttaatatgg tgagtatgta caaacgtact gacgaactag
    14341 cgaatgtact gtccgaaagg catagctcct agaaatggga gagacagtga gcaaaaaatc
    14401 actcattgtg ctaacttgtg gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt
    14461 acaggcgtag gagaagttag ggggcaaaag ctagcatcta agttaaggca tgtggataga
    14521 tataagggaa caggggaagc catcaacatg gagagtgcat actcgatgaa gtggtagtaa
    14581 gaaatatgtg aatgacaaat aacttacttt agtgatggtg aaagtagagt cacgactgat
    14641 gaaggttctg taatggaact ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc
    14701 cgttcaacca tgggattctc gtatgactaa aaaggtcacg agccaattgg agcgtgataa
    14761 accatgacaa aagaaacaag tccaaaagat aggaagttgc ggcatgcaga atattatggc
    14821 atgacggaaa tcttcgataa gatatactcc aaaagtttga agggagataa tttcaagaat
    14881 ctaatgcaga ttatcatttc tgataacaac attgtcctag catatcgtaa tatcaagaga
    14941 aatcacggta gtgtgacaag aggagaagat ggagtaacga ttaaagatat tgaaaatctc
    15001 aaccgtaatg aattcgtaga aattgttagg aagcgattcg cacattataa ccctcggaaa
    15061 gtaaggcgtg tagaaattcc taaatataat ggtaaattaa gaccgttggg tattccatca
    15121 atatgggata gaatcgcgca gcaatgcatc ttacaagtat tagaaccaat atgtgaagca
    15181 aaattcaata atcacagcta tggtttccgc ccaaatagat cagcagaaca cgcaatagca
    15241 gattgtatgg cccgaatgaa tcaaagtcac atggaatatg tggtagatgt agatatcaaa
    15301 ggtttcttcg acgaagtcaa tcatactaaa ctcatgagac aaatctggtc tatgggtata
    15361 cgagacaaac agcttctctt aatcattagg aaaatgttaa gagcacccgt agtacttcca
    15421 aatgggaagt tgatataccc aacaaaagga acaccacaag gcggaatact tagcccatta
    15481 cttgcgaaca tcaatctcaa cgaatttgac tggtggatag caaatcagtg ggaggaaaaa
    15541 gatgccaaag agctaaaaca tcattttagg aaaaatggag cttgggataa aggtaatatc
    15601 tatcacaagc tcagaaagag tacaactcta aaagaaatat acctagtgag atacgcagat
    15661 gattttaaaa ttttctgtag aaattataat gaagcgaaaa aagttttcta tgcaactaaa
    15721 atgtggttac aggagcgtct aaagttacca atatctgaag aaaagtcaaa aatcacaaat
    15781 ctttcaaaag aagaaagtga atttttaggc tttacgatga aattagtgac caaaggtaac
    15841 aaaaaggttg ttaatacgca tataggtaca aaagcacaaa gcatgattaa agtgaaacta
    15901 aaaaaacaaa tcaaacgaat tcaaaaaagt cctaatagca aagatacctt tatctatatt
    15961 ggagtctata acagtatggt tattgggatt cataattact tcaaaattgc gactcattgt
    16021 agtgtagcaa tgcgaaaaat tgcaagagaa ctcagaccaa gttttgaaat acgactacga
    16081 gaacaaggaa tctcaaaaga aggacaatac ggaaagtacg aaggatacaa gccttaccta
    16141 cggtcaggac aaatacgata tataagaggg tttccaatat taccagtagg gtatattcaa
    16201 cacagacatc cgatgtgtaa aaaaagaaat attaataaat acaccgaaga aggtagggaa
    16261 gccattcata agaagcaaca aaccgtagaa gaatggaaga tatcgtggtt gtgtagcaat
    16321 ccagtgctta gtaagagagc aacgattgaa tttgcggaca atcgaatttc aaaattcatc
    16381 gctcaaaagg gaagatgtgc agtaacaggt gaggagctta tcttatctga aatgcattgt
    16441 catcatatta tcccttatca tgaaagcaaa agtgatagct atgaaaacct agtgattgtt
    16501 actgaggagg ttcatcgggt aatacacgct acacaaagtg aaacgattga ggaacttcta
    16561 aaatatctaa aattaaatcc aaaacaaaaa gaaaaactca atgagttgag attaaaagtt
    16621 ggtaacgaag aaatatctta ataaatattt gtaggacggg aaaacagaat gtcttttata
    16681 gacgagggaa cgccgtatgc ggtgaaagtc gcacgtacgg tgtggactgg gggaaaagac
    16741 ggagataata tcaaactctt acctatcagt atcgattaat ccagatttta ttgaaacggt
    16801 aaattatgtc acaaactttc aacgagaaat gcgtaaagta gaagcaatga ctgttttagc
    16861 tacacaatcc ccacaagaaa tggtgccaga aaatatgtct tctgataact tctcgaaact
    16921 gaagaaagtc tttgaatttt cttctacgaa gatctttatg cgtatggatg aatctatttt
    16981 acgtcatata gaaggattag tgggtaaatc aatgacgaac tcagaattag aatccattcc
    17041 tcaacaaaat caaggagacg ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca
    17101 ttttagtccg aacaaacgtc aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa
    17161 aatgaaagtt taatcaagca gtatgttaag cgtcgtgcaa agcggcgctt atttttgtgg
    17221 ttgtttggta cttctgcagg tttgattact attttgatca ctgtgttcgt gaccctattt
    17281 ctaatacttg cggcgggctc gattgataat tcggattcag attcaagcag tggtggcgaa
    17341 gcttttacag gagaatattc ggaaggctta ccgatttata aagaaatcaa aggaagaggc
    17401 cctttttctg atgagattgc gcaatatgcc gtaggtgcag cagtgaaata taaattatta
    17461 ccaagtgtga tcctttcgca atacggttac gaatcagcgt ttggtacatc ggcgtccgca
    17521 agaaatgatc tcaactattt tgggattact tggtttgatg gttgtctgtt tcctaaagga
    17581 actgcacgag gaattggtgg cattgaaggt ggctggtaca tgaagtttcc caactcaaaa
    17641 gctgcatttt cctattacgg ctttatggta gccacacaat ccaattttaa tgcttgtgta
    17701 ggaaataaaa gccctggcgc aagcttattg atcttaggac gaggcggtta tgccgctgca
    17761 ggaattacgg aagatagtcc gtattataca ggttgtatgt caattatcac ttctaataaa
    17821 ttaaccgaat atgatgaatt cgccatcaaa cattggggtg aaggtggcaa taacaacggc
    17881 acgattactg gtgaatggac gaatccattc cctggcagca gtttagataa aagttccttc
    17941 agcggtggtc aattatttgg aacgaatcca ggcggtgaat tccgtccgaa cggcttccat
    18001 gatggattag attttggttc agttgatcat ccaggaagtg agattcatgc ggttcatggc
    18061 ggaaaagtcg tgtacgttgg caatcctggt atttcaggtt tgggcgcgtg tgtgatcgtg
    18121 attaattacg atggcttgaa tatggtgtat caagaatttg caaatagtac aggaaattct
    18181 cgagtgaaag taggcgatca agtgaaagtt ggtcaagtaa ttgggatacg agatacagca
    18241 cacttgcatt taggatttac tcgaatggat tggcgtcaag cacaaggaca tgcattcatt
    18301 gatgatggta catggatcga tccgttacca tttttgaata gcagtaaaaa atagatcagg
    18361 agaaaaaata tggaaaataa aacgttaaaa atcgtcttaa cagctgcaat tagcgttagt
    18421 ttaatcggca atattgcgtt gttttggcag ctgaaagagg aacaagcagc aaatacctat
    18481 aaaattgaag aagtggctaa aaacgcgtct gagaagctca aaaaaaattc ctctgaggca
    18541 caggcgaaga tcgacaagtt aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag
    18601 ctgaagcaac aagatggcac aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg
    18661 gcagaagagt tcggtcaatt ggccattgat aaatcattaa gcagaaatga attaacggaa
    18721 aaactaaaag atgtcgcaac ccaagaagtg atcgacaaat taagtcctac agacgatgat
    18781 caccaacatg atgattacgg aagttactcc tttacattag gtgacgtgca aacttacgct
    18841 caaacaagca gtgtaaaaga ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc
    18901 aatccgcaat tcaaagatgt caaaccacaa aaaataaaag gtggattaac tgtcactgaa
    18961 aaacaagtag atggtcaatg gaaagtcata gactttagtt atttcactcg ttaggaggaa
    19021 gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa gttatcttag
    19081 atggctggga atctttatga ttctcggctt tttgattctt gctagtccga tttatacgaa
    19141 tcagaaggta gatataccag atgaggtaag cacgaacgcc tttttaaaca cgaaagagct
    19201 gacaatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat ttgcgatcga
    19261 tgatgaagat caaagcttgt taaaagaact ggctaatctt aattatcgcg tagaagtaaa
    19321 gacagctaaa ggggattatc aagcaatacg agccaaagtg attaaggtgt ctgatgatta
    19381 ttttgcagtg aagctaacgc atgtaccaga agaatatatc gccatgcgtt taaccattat
    19441 tcctgaaaaa attgatccaa aagtggatat gcaagaacca caagatctga tttattacgt
    19501 gcacgaagat aaggttaaag atcaagtgaa agatgaagat tatgaacaac acgcgatcaa
    19561 ttacaaagtg aaaggataca agaaagaaca aaaagcagct caaaaacaaa tggaatcttt
    19621 ccaagcaacc attgatttga atgaagaatt agtgaagaag ctaaaagatc aattgcctta
    19681 tcaagtcgca gatgatcagg aaaatactga aaacaagatt aatagctacc agcaagaaat
    19741 tgaaacatcg aaaacgcaac tgaaagatca agaagagatc attcaaaaac ttcaagaaaa
    19801 aattgatcgt atgacaaaag aaaagattta gaaggacagg tggagtgaaa aaatgaagcg
    19861 aacaaacttg gctgacaaag atactttcgc tgagcagaaa gtattgatta aagcctatct
    19921 caaagttgta aaagctaaag aaatatgtcg agaagtgaat ttatctaaca caacaatatc
    19981 tgagttgaag aatggtcgaa agaatttaga tactttaaaa ttcagatcat ttgaagctct
    20041 atatgtagct gctaaaaaat attttacagc tgataacaaa taaaaaacag gagtatttta
    20101 tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat atcaccttaa ccatttgtta
    20161 atgagatggg agaaaacatt aaccacgatt ctaacgcaaa catcaagcac ataaaacatt
    20221 taacactcct cctttctggc tgaagatttc aaccaaacag gaagacagcg tatcaattat
    20281 agcatacact ttttcttaaa ttcatttttc atatgacaaa atatttcctt ctaatcgaca
    20341 aagttagaag gattttttat tgtctaaata cacaaataac cttaattttt aaagagcata
    20401 aggaaacgtg atatcacttt ctcacgaaag gtgttaggta ggctgtgcct accacgtttc
    20461 taagaaagat aaatgatatc aaaattttgg atttgtgtgt agatttgtgg gtaggatgaa
    20521 agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa aatcaaaaaa t
//
LOCUS       DAFCLG010000040        20285 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFCLG010000040 DAFCLG010000000
VERSION     DAFCLG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.040"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="LND56_001364"
     CDS             140..460
                     /locus_tag="LND56_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743296.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="LND56_001365"
     CDS             912..1112
                     /locus_tag="LND56_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6743297.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="LND56_001366"
     CDS             1259..4048
                     /locus_tag="LND56_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6743298.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="LND56_001367"
     CDS             4095..4622
                     /locus_tag="LND56_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBK6743299.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="LND56_001368"
     CDS             4643..5833
                     /locus_tag="LND56_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6743300.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="LND56_001369"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="LND56_001369"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK6743301.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="LND56_001370"
     CDS             8224..8898
                     /locus_tag="LND56_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6743302.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="LND56_001371"
     CDS             8895..9236
                     /locus_tag="LND56_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743303.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="LND56_001372"
     CDS             9266..9625
                     /locus_tag="LND56_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK6743304.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="LND56_001373"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="LND56_001373"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6743305.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="LND56_001374"
     CDS             12541..14007
                     /locus_tag="LND56_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6743306.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14622..15161
                     /locus_tag="LND56_001375"
     CDS             14622..15161
                     /locus_tag="LND56_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBK6743307.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15235..15675
                     /locus_tag="LND56_001376"
     CDS             15235..15675
                     /locus_tag="LND56_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBK6743308.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15688..15897
                     /gene="copZ"
                     /locus_tag="LND56_001377"
     CDS             15688..15897
                     /gene="copZ"
                     /locus_tag="LND56_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBK6743309.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15897..18083
                     /locus_tag="LND56_001378"
     CDS             15897..18083
                     /locus_tag="LND56_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBK6743310.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18103..20274
                     /gene="copB"
                     /locus_tag="LND56_001379"
     CDS             18103..20274
                     /gene="copB"
                     /locus_tag="LND56_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBK6743311.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6777 a         3527 c         4407 g         5574 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aagtttcggc ttgtttttcg cagaagctac ttctggaaca ccgtgaatca gaggttgtga
    14161 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagcccc tcatattttt
    14221 cgttttgctg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggta
    14281 gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacaa cttctcatgt
    14341 ttgttgacta ttgtgccttg tgaccgaggt gttggcagga gaacaccgtt taaggaagag
    14401 gttgtaaaat cagcgatttg acctgagtat tagaggaaca aacgaaaaaa tagctcctca
    14461 tattttgccg ttttttgagt taatgctgaa ggtcagctga ttgaacacca tttattcgaa
    14521 acaagggact ttgacacgac ttttgtcaca gtcccttgtt tttttgcggc gttataagtg
    14581 gtatgatcga accaaattcc aaagagaagt gagggagaag aatgaagatc atcgtgattg
    14641 gacaaagtgg gtcgggaaaa tcgaccctcg caaggaagat caaagaaatc accgactttc
    14701 cgctactgcc gctagacctt ttatggcata caacagacta cagtattcag gcaaaaaagt
    14761 ggtttttgca agaacagcaa tcgtttatgg tatctaatcc gtcatggatt gttgaaggaa
    14821 attatacttc tacattagag gaaaggatta aagaagcaga taagatcatt tggcttaaag
    14881 tgccgcgtta tctcgccatc taccgagtag tttggcgttc tctaaaaaga aagataaaca
    14941 aaaaatctcg gccagatatg cctgaatcat tttcagagaa actaaacagg gaatacttag
    15001 aatttttatc ttttatctgg cattttgaag aaaacaatga accgaaaata cagcagctga
    15061 tccaagtagc taatgcaaga aataaactga tcatccttaa aacaagacga gagaaacaag
    15121 aatttttaac taaattgaaa atagagagca aaaataattg acagtttttg tttacaagcg
    15181 taatctgtat tcataagcta atgattacat ttgtaaacga aaggtgtgaa ataaatggaa
    15241 aaagtcaaaa cgccagtaaa gatcagtgat tcagagtggg agatcatgcg tgttatttgg
    15301 acacttggac agacgacagc ccaagaaatc acgcagatct tagctgattc gatggattgg
    15361 aaaccagcaa cgatcaaaac tttgctggga cgtttggtaa aaaaagaagt gatctggaca
    15421 gaacaagaag gaaaaaaatt catttatcat ccagctgtct cggagatgga aacagttcgc
    15481 agtgcgacgg aaaatctttt ttcccatatc tgtgcaaaaa ggattggaga gacgattgcg
    15541 gatctagtag aagaagccac tttgactcaa gaagatatca atgagatttt gaaacaactg
    15601 gaacaaaagc aaccagtaga tacgatcgaa tgtaactgta tcccaggtca atgtgaatgt
    15661 aaacaacatc aataggagga agaaaaaatg aaacaacaat tttcaatcga aggtatgagc
    15721 tgcaatcatt gtgcagcaag agtagaagaa acagtaagcg cacttgacgg cgtgcaaaaa
    15781 gtgaaagtaa atttgaaaaa agcaaacggt acggtcaagt ttgatgaaac aaaagttcaa
    15841 tccgaaaaga tttgtcaagc aatcaatgga ttgggttata aagcagaggt gatttgatgg
    15901 caacagcaga aaccaaaatg gatacgttcg tcattacagg tatgacttgt gcaaattgtt
    15961 ctgctaggat cgaaaaagag ctgaacggac aacctggtgt ggtcaatgcc acagtcaact
    16021 tggccactga aaaagcaagt gtcaaatatg aagggacgac cactgaaaaa ttaatccaaa
    16081 gcgtagaaaa catcggttat ggtgcaattt tgtacgatga agcccacaaa caaaaaattg
    16141 cagaagaaaa gcaagcgtat ttgaaaaaaa tgctattcga tcttatattg agtacggttt
    16201 tgactttgcc gttgatgttg tcaatgattg ctatgatgtt aggaagccat gcggcgattg
    16261 ttcacttttt tcattttcca atcgtccagt tagtgctgtc tgctcctgtc caattttatg
    16321 tcggtgctcg tttttataaa ggtgcgtacc atgcaatcaa gacgaaagca ccaaatatgg
    16381 atgtattggt agcaattgga acatcggctg cctttgcatt gagcatttac aacggatttt
    16441 tccgtggaca cccccaagat ctttattttg aaagcagcag tatgatcatc acgctgattt
    16501 tgctcggcaa atatttggaa catacagcaa aaacgaaaac aggtaatgca atcaaacaat
    16561 tgatgtctct tcaaacaaaa accgcccaag tgataagaaa cgggaaagaa gaaacgttag
    16621 cgattgaaga agtagtcgtt ggtgatcaac tagtcatccg tcctggtgag cagattccag
    16681 cagatggtcg aattatttca ggaagcagtg cgatagatga aagtatgctt actggtgaaa
    16741 atcttcctgt agaaaagaat ccggatgaca cgctttttgg cgggacaatc aatacgaatg
    16801 gtctgctaca catggaagtc acacaagtcg gcaagcagac agtattagcg caaatcattc
    16861 aaatggtgga agatgctcaa gggagcaaag ccccaatcca aaaaatcgca gatcggatct
    16921 cagggatttt tgttccgatc gtattagtca tcgcgtttat cacgctcata gctacaggtt
    16981 tgatcactgg tgattggcaa ctcgcattga tccatagtgt ctctgtatta gtcattgcgt
    17041 gtccatgtgc attaggttta gcgaccccaa cagctattat ggtaggaacg ggtgttggtg
    17101 cacgaaatgg aattttaatt aaaggcggag aagcattaga agcagctgct cacttagaca
    17161 gcattgtttt ggataaaact ggaacgatta cagaaggaaa gcccaaagtt acagatctag
    17221 tcggttcaaa agaagtccta tctatttttt atactttaga acaagcttct gaacatccac
    17281 taggtaaagc aatcgtggaa tacggaaaac tgcaagaagc agcaacatac gatatgattg
    17341 attttacagc ccacccaggt gctgggatca gcggtacgat taatggagta cgctattttg
    17401 cgggtacacg taaacgatta attgaattga acctatcatt tgatgaatac caagaacacg
    17461 cgttagaatt agaacagcag ggaaaaacag tcatgttttt agctgatgaa aagcaagtga
    17521 tcgggtggat tgccgtggct gatcaaatca aactagaagc caaacaagca atcaagcagc
    17581 tccagaataa aggattagat gtttttatgc tgacaggaga caacaaactg gcagccgaaa
    17641 cgattggtaa acaagttggt attgatccaa agcatatctt tgctgaagta cttccagagg
    17701 ataaagcagc ctacgtagaa aaacttcaga aagatggcaa aaaagtcggt atggcaggcg
    17761 atggaatcaa tgatgcaccg gccttagcat tagctgatgt cggtatggca atgggaagtg
    17821 gaacggacat tgcgatggaa acagccgatg tgacgctgat gaatagcagt ttggcatcca
    17881 ttgcacaaac aatcgagttg tctaggttga cattgagaaa aatcaaacaa aatcttttct
    17941 gggcttttgt ctataatacg attggtattc cttttgccgc actaggcttt ttgaatccaa
    18001 tcattgcagg aggagcgatg gcgtttagtt ccgtcagtgt attgttgaac tccctgagct
    18061 taaatcgaca catgaaaaag taagagaagg gatggagtaa aaatgaatgg gaacgatatg
    18121 gagaaaaaac atgagcataa aaaagaagta gtaaacaaag aaagtacaga atctcatgaa
    18181 aaacagatgg agatgaaaca tgaccatagt cagatggatc attccatgca catgggacat
    18241 aaccatggag atatagcaaa cagcaaacaa atggaccatt cgatgcacat ggatcacgaa
    18301 cacggcggca tggatcattc catgcacatg gggaatttca aacaaaaatt ttggctatcg
    18361 cttatcctag ctatcccaat cattcttttt tcaccaatga tgggaatgga atttccattt
    18421 caagtaacgt ttcctggttc tgattggctc gtattgatct tagcaactat actatttatc
    18481 tatggcggtc agccattttt aagtggagct aagatggaat tgaaacaaaa aagtcctgct
    18541 atgatgacac tgatcgcgat ggggattaca gttgcatata tttatagtgt ctattcgttt
    18601 atcgcaaatc tgctcaatcc acatacacac gtcatggatt tcttctggga attggctact
    18661 ttgatcgtta ttatgctact aggtcactgg attgaaatga atgctgtgtc gaatgccagc
    18721 aatgctttac aaaaactagc agaattactg ccagaatcag tgaaacgctt gacgaaagat
    18781 ggaaaagaag agacagtttc attgaaagaa gtaaacgaag gtgatcgttt gatcgtccgg
    18841 tctggtgata aaatgccaac agatggtgta atcttgaaag gtgaaacaat tgttgacgaa
    18901 tcagctgtga ctggcgaatc aagaggaatc aaaaaacaag ccaatgacaa agtgattggc
    18961 ggttctatca atggggatgg aacgatcgaa atcgaagtaa ctggaactgg tgaaaatggt
    19021 tatctagcga aggttatgga aatggtgcga aaagcacaag gagaaaaatc caagctagag
    19081 tcattatctg acaaagtggc aaaatggctg ttttatgtcg cattaattgt gggtatcctg
    19141 gcattcatcg cctggttgtt cttgacagat cttcctaatg cattagaacg tatggtaaca
    19201 gtctttatca tcgcctgtcc tcatgctcta ggtttagcta tcccattagt tgttgctcgc
    19261 tccacatcaa tagcagctaa aaacggatta cttttgaaaa accgcaatgc actggagcaa
    19321 gccaatgatc tagatgtgat catgctggac aaaacaggaa cactaacaga aggaaaattt
    19381 actgtaacgg gtgtcgaagt acttgatgat gcgttcaata aaaatgaaat ccttcaatat
    19441 ctaggagcct tggaagcgaa tgccaatcat ccattggctg ttggcattat gaattatttg
    19501 aaagaacatg aaatcaagcc ttatcaagct gaaaatctaa aaaatctttc aggagtcggg
    19561 ctagaggcaa cagtcaaaaa acaacaagtg aaaatagtta atgaaaaaga agttgagcgt
    19621 ctcggattaa acgtagaaca agcattattg aaaccttacc aagaacaagg aaataccatc
    19681 agcttcctga ttttagaaaa tcatctagct gcaatcgttg ctttaggtga cgtagtaaaa
    19741 acagaagcaa aagaatttat ccgaacattg aaagaaagaa aaatcactcc tgtcatgctt
    19801 actggtgaca ataaaaacgc tgcccaagca gtagcagatt atttagggat cgaagaatat
    19861 tatggcggtt tattaccaga tgataaagaa gctgttgtcc agaaatacct tgaccaaggg
    19921 aaaaaagtaa tcatggtggg tgatgggatc aatgatgcgc caagtttagc ccgtgcttca
    19981 atcgggatgg caatcggtgc agggacagat atcgcaatcg attctgcgga tgtcgtattg
    20041 acaaacagcg atccaaaaga tattttgcat tttcttgatc tagccaagca aacgcgaaag
    20101 aaaatgatcc agaacctctg gtggggggca ggttacaata ttcttgcaat tccgctagca
    20161 gccggaattc ttgcgccaat tggtatcata ttaagtccag cagttggtgc tgtcttgatg
    20221 tcgttaagta cggtagttgt agctctaaat gctttgacat tgaaaatcaa ataataatga
    20281 aaaaa
//
LOCUS       DAFCLG010000041        20209 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFCLG010000041 DAFCLG010000000
VERSION     DAFCLG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.041"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(119..547)
                     /locus_tag="LND56_001380"
     CDS             complement(119..547)
                     /locus_tag="LND56_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743312.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(575..1213)
                     /locus_tag="LND56_001381"
     CDS             complement(575..1213)
                     /locus_tag="LND56_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743313.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1217..3199)
                     /locus_tag="LND56_001382"
     CDS             complement(1217..3199)
                     /locus_tag="LND56_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBK6743314.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3379..4044
                     /locus_tag="LND56_001383"
     CDS             3379..4044
                     /locus_tag="LND56_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743315.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5979..9128)
                     /locus_tag="LND56_001384"
     CDS             complement(5979..9128)
                     /locus_tag="LND56_001384"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HBK6743316.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9141..10340)
                     /locus_tag="LND56_001385"
     CDS             complement(9141..10340)
                     /locus_tag="LND56_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBK6743317.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10330..11925)
                     /locus_tag="LND56_001386"
     CDS             complement(10330..11925)
                     /locus_tag="LND56_001386"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK6743318.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12509..13216)
                     /locus_tag="LND56_001387"
     CDS             complement(12509..13216)
                     /locus_tag="LND56_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743319.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13281..14117)
                     /locus_tag="LND56_001388"
     CDS             complement(13281..14117)
                     /locus_tag="LND56_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6743320.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14135..15544)
                     /locus_tag="LND56_001389"
     CDS             complement(14135..15544)
                     /locus_tag="LND56_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6743321.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15558..17381)
                     /locus_tag="LND56_001390"
     CDS             complement(15558..17381)
                     /locus_tag="LND56_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6743322.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18224..18550)
                     /locus_tag="LND56_001391"
     CDS             complement(18224..18550)
                     /locus_tag="LND56_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743323.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18596..18958)
                     /locus_tag="LND56_001392"
     CDS             complement(18596..18958)
                     /locus_tag="LND56_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBK6743324.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18995..19210)
                     /locus_tag="LND56_001393"
     CDS             complement(18995..19210)
                     /locus_tag="LND56_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743325.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19337..19672
                     /locus_tag="LND56_001394"
     CDS             19337..19672
                     /locus_tag="LND56_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743326.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19748..>20209)
                     /locus_tag="LND56_001395"
     CDS             complement(19748..>20209)
                     /locus_tag="LND56_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743327.1"
                     /translation="FNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLM
                     TEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSF
                     LIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         6189 a         4076 c         3155 g         6789 t
ORIGIN      
        1 tgtttttatt atgatatatt tttatacaac taaaaatgaa tatattaaaa ctaccaaaaa
       61 taacttttaa cgctgtttca ttatatgttt tttctatttt atataaactt ttggatactt
      121 aataccaacc ggttggattg taccaacatg agaatgtgtc agttctattt cctgtcgcac
      181 ttgcataggc ccatactcct tttgttgcat ttgctgatcg aatagagttt ccaactttcg
      241 cttttgcacc gtgacgatta tagacacgac tatagtggtt agaatgacct gtttttctcg
      301 tacaagttgt atcactataa taaacccatc ttccagagct atgattaatt gctgttctag
      361 aaatttcccg gtctactggt aaatttgact taataatatt acctttagca tcaaaatgaa
      421 tctctcctgg ctttaaagat ttgtaataga tttcaccgtg aactgaattt gcttccactt
      481 cagatgacat agcgtttcct ccaacaaaac ctatccctag tgcacaaata ataactgctt
      541 ttttcataaa acgacatcct ttcctttttg atttttaaag catgatcttc ttgtttccta
      601 ttttggtcag atatggatca tgcgtgacta cgataactaa cgtaccttgg gaagcttgtt
      661 gcgataagat ttcaactact ttatccttat ttcttttatc caatgaccca gtcggttcat
      721 cggctaaaat gattttgcta ttcttcaata agactctcgc acaggcgatt ctttgcttct
      781 caccaccaga taatgtgtag ataggcgtac ttagcttctt gtttatattt acttttttca
      841 atgcctcata ctttgctgtt cgcttatctg aaacatttaa attcgaaaac ttcaatccaa
      901 tatctaaatt aaaatcgatt gtttcatatt caatcaaccc aaaatcttga aaaagaaaag
      961 atatggtatt tctttttatc aagcgcttct ctctcccagt taattgtttc gtctcttgat
     1021 cgaacaaata gaattgtcct gaactgaact gatcattcaa tcctaatata ttcaataacg
     1081 tacttttacc tgctccactt tctccaacaa cagtcacaag atccccagca tccgcagtga
     1141 aacgatccaa atgaagaatc gttttgtttc gaatgatttt tttcgcacct tttatcacta
     1201 tcaacggctc caattgttaa cctccattca tcagcaataa ctgacttttc ttttctgtag
     1261 acaaaataaa aacaactgaa agaataaaat caatgaagct aaagaaaaaa atattaaaaa
     1321 gatatttagg ctctctttct ataagaaaaa ggaagattac acttgtcgtt ccccatttga
     1381 tgaggttcat catgaagaaa tctttatacg tttcaaagag cgaataacca cttgaacgaa
     1441 agatagcgat tttccgacaa ttgtagacaa aatatatgca taacatgaca tagctcaaca
     1501 agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa gtaatgaaat gatctgctta
     1561 acactttgac agctatctct tcagtctgag tatacggaaa tgataactgc aagttatcac
     1621 gtaactgact ttcttcaatc agctcaaata acctttcttt cgacaaagag cctatcttga
     1681 tctttaacgg tggatagatt tctccgctga gaatatttct ttccaaatac atactgtttt
     1741 tcaatgtcag tatttcaaca accggataat gttcgatcca ggggatagaa ggaataaatg
     1801 tataaatggg ttgattgttt tttataaata gaaatttcgg ttctttttca ctgatttctt
     1861 gataatattc tgttatttgc gccaggtcat tttttaaatc tgtatgtcta tatcgttcag
     1921 ggatcaatat aacagggtgc gtttcttttt cggttatttg gatcaacctt ccagagacat
     1981 caatcaaagg aaatttctta agataattca gatttatttt tatccctctg ccaaactgtt
     2041 cattttcttc attcaagtaa tctgatacat tgactaatat acttccgttt ttattcaaag
     2101 tttcatataa ttcctgatct gctgctgatg caaaacgatt ggaataaata aaatccacgt
     2161 ggtttttccc gatggttact ggataaaata catgatagtc ctcaccagcg atttcttttg
     2221 gtggttcatg ggaaacagct aactgtgaag aagaaaaaac gatagttgct aattcgacat
     2281 tggcaacggt caaaagaaga gaaaacaaca atccaaatgt aaaaagaatg tattggatat
     2341 agttgaacgc ttctcttaac gaagcgattt tttcaactaa taaaagagaa atcaaagaga
     2401 taccatatat tccgctgaac aagaaaacgt acatctggaa caaataaaga aattgctcct
     2461 tataatacat aaaaataaaa ccaccaatga ttcctgcaat tatgaatgta ataatgattt
     2521 tctttttccc caataaacac cacaatactt gatgtaatga aaagtcatta ttcaacaata
     2581 aactgatttc tttattcatc aataagaaaa aggatgataa caaaaggaat aaaagaatca
     2641 atccaatagt taaatacact cgataattat atacgtctgt atttgaaata accgaatacg
     2701 aatagctatt ttcaaagtct tcaaaaatat tagtggcata atcagtgtga aatgcttgat
     2761 tgaatcgatc agtgaatagt gaaatgaccc gttcataggc ttctttttct ctcgtcatca
     2821 agaaaaattc accttcaaaa tcttttattt tgatcgattc ttcaatggac gttacgcgaa
     2881 aggaggtatt agaatagggt accttctcgt gagaagattt gacatcccct tgataataca
     2941 atgtcatttc aagctttggc tgtatcttga agttccaaga tgtatctact tcattggatt
     3001 catttactgt ttttttcata taagaaagct cagtttctga tgagacattt tcaaatacca
     3061 tgattagctg ctcatattct tcttgcccaa taaaatgttg tggcaatcga atcggagtat
     3121 aagtatctgt ttgatggaaa aattcatttg tccgatagag tacctgttta tttatactaa
     3181 ccaaggcaaa accactcatg ataccggtca ctatgattgt gagtaaaact gtgaactttt
     3241 ttttgattat caccactctc ccttacttaa atgattcttt gataaaatca taccgttttt
     3301 catcaaaaaa acgtatctat caagtgtata ggtcatgtag aataaatcta tatactgtag
     3361 aatcgaaagg agaagaagat gaacaccgga gaaaaaatac gaacttaccg cagaaattgt
     3421 aagcttacgc aaaaagaatt aggcgaaaaa attggtgttt ccgataaaac gatttctagt
     3481 tgggaaaatt ctcgtacaat gccagacttt gaaatgctgt cacttttaca tcatacttta
     3541 ggtctgccga ttgattttcc ttctattgaa tcagctaccg aatcaataga accaacattg
     3601 ccagatgaac ctcttgttaa caagaaatct tattcatcaa aacaaccctt ctcactcaaa
     3661 aagacactat ctttctttct gctcgtcttt tgtttggtct atctttttct tatcaattat
     3721 caatacaaaa atgaaacgat cgatcgattc aatccttttt tgaaaccaca aatcggctat
     3781 accacactcc ctgtaggata cgtggacaat tccatcgaac gttgggtatg taatgatatc
     3841 tttggccatg gtacatggct gtcttttcac gctggagagg tagaaaaaga gaaaaaccat
     3901 gctgtaatca aacacaaagg aaaacacgtc atgaacgttc gcctgctcac ttgggaagcc
     3961 attccctcag attaccaaga aatcctcagc caagactatg acgacgcctt aaaaagaagc
     4021 cctcaggagc cagataggca ttaagtagga gatgaacgta gagatagaaa aaatcaatgc
     4081 acatgatctt atccaaaaaa caggggtaca aattaattga aaaagccttg agatgaaagt
     4141 tcctaaataa aaaagttcag aatgataggt atttctggct tatagtataa tatttacgcc
     4201 acagcctccc aatgattctt tttggaatca taatcaagga tggacattca caaaaatatt
     4261 ttgctcattc gatccgaata gattaacgac caatctagta agtaatatag attggtacgt
     4321 agtcaagctg cacataattt agatcaattt tagttctttt ttatatttct atgatctgct
     4381 catttacttt tatatgatgg cttacagtaa cataaataaa tatgataatc gctggtagtt
     4441 tatccaagag tactcaataa aatacaaaat ggattgttca agcgcctatc ataatgataa
     4501 aatgaaacaa ataaacgata caaggaggaa gagactttga gtaatggcag aacaattttt
     4561 tcattgtaaa aattgttcag ttgttaatct atgggatttc ggtagcatgg gcattttttt
     4621 atagaggaat cacaccaacc tggccgtttt atctcttgtt agtgggaata ttagtaggaa
     4681 attttgcccc tcttcactat cgtacgaact tttttccaac tgattctggc ctttttctta
     4741 aaacaaagac gcgaaaatta gaaaacatta ttgaaatgac aattacctgt ataatcattt
     4801 tcttagttat attcctaaac gtcttctaat aagaatctct taaaattagc ttgttacagt
     4861 tagttaccta gtttatataa cacagttatc ttatcttcct tatcaacatc ttttgccaac
     4921 tctttgtctt aaattcgaaa catataagca gagctacgaa caccaagact gtttcggctc
     4981 tgttttttgc atcaaattca ctcttatata gctgataatc tcggctcttt aaaagaaatt
     5041 attttcgctt cattttatta taaaaaggct aggaaccttg atgttatcag gttcctagcc
     5101 tttatttaag tattacccca cagacccttc catatagatt tttaaattac acactagttt
     5161 aaactaaaac aaacaaggaa aaacttacca tttaatcaaa aaattagctc atatgaatat
     5221 attagaatgt aaattagact ggtcaaaatc tggtcaaaaa agaggacgaa tacaggtagt
     5281 cctctttttt gttattttga tattctaaat cattttttat tggattattc ccttgttcga
     5341 tatagaccca aattgatcta tcaataatta tcaaactgag tcatatagag acaaaaattt
     5401 caaaaatgac ctgttaaatg gtcatcgaat atcatttaaa atcaatacga cggagtcaga
     5461 cacggagtca aaacaagccc gctgagagta aaaaatatac agtcccatag gtaaagtgcc
     5521 acaggactgt atattttttt atttgagatt acctgaaatg attccattta atttttatct
     5581 cattttttcc tactagttag tatcaaatac tctcctattt tctcaataaa ctattttggg
     5641 ttacatggta tctgaaacag ctaaaacctt gtcattatag caaaaaatca ctttatctcc
     5701 gtttcatttc agatacattt cacttaagcg gattccaaaa aaacaaaaga aaaaggaacc
     5761 cctagttagc ctatgctttt tattggttcc tctcaaaaca cggtcatata acaacggggt
     5821 ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa tagctgtctt aaatacttcg
     5881 ttataaaatg tgaacaaata aaagacatct atccctattt tctacacttc cacttgacta
     5941 ccatctttga atcgtacaat tttcttaact cttctcgttt acctcaattc attctcaagt
     6001 ggaatcaact catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta
     6061 aatggcttcg cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt
     6121 gctttcatat taggaattgg atcttcacct ggtagatatt gcagattaga agcatgtaat
     6181 tctttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc actcgcttgt
     6241 gtaaaatagc gacgtttaat gacaaagata tcttcattac tatctaaagt ccccacatca
     6301 aatgcattta aaatttgtcg gtaaacttct ttgactcctt ctgttttatt caatttagcc
     6361 aaagcttttt caatttcttc tcgtgcatct gaactattcg cagcataagt tcctagtaat
     6421 tgatcaatat aagttgaatc aatatcatac aacttagtgg tattttcact ataaaattcg
     6481 atcgttgaaa aatcttctcc ttctttttct ggttcttcct ctattaatga acccttcacg
     6541 gtctcgtaca cgccaatttg ttcttccaaa agttgaattt gattgttcaa agttggagat
     6601 ttcaccatgt catcattgta atcgtcataa gtcactaacg attcataagc acgattcaat
     6661 tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc atcaatggat
     6721 tccatggatg aaacctcttt taacttacca tatgcttgtt tgaatctttt tttggattcc
     6781 tgataacttg gataaatcaa acctgattct tcttttgctt ttgaatacag ttttgtcgca
     6841 tcctcgacat tcttctccat tgtcgctggc ttacgaaacg tcacaatcaa tccttttgtc
     6901 ttatcaggat acgtacgatt cgtacgtgaa aaagcttgga tcaatcctgc atactctaga
     6961 ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa aacctgttaa tagacgatcc
     7021 actacaatcg ctaaatcgat ttgtttccca aattgtttga attccgctct ttttcgtgcc
     7081 aaacgattat tgatgtctcc gttataacgc tcaatgtctg ctaatgacca ggaagtatca
     7141 taatactgat tatattcctc aataattttt tgcatttctt cttgttgggc acttgaatct
     7201 cggctatttt cttctaatga ataggtaata gcgattcgtg gaaaatcagg atcctccatc
     7261 gttcgtcctt cacgaatgag ctgattggga aactcttttg ttaaccaatc aggctctttc
     7321 gtcatctctt tgatggcccg ataatacttt ttggccatgt caatggaact cgtggttaag
     7381 attgcagatt tcgttggcct gccatttcga aaatcaaatt ttgtataggc attatctgga
     7441 cggaacattt tatgaatgac tttttggata tgttcatctg cttcaaaaac agctgggtcc
     7501 aaataacttt cttttttgac aggttccatt tgatcaatca tacgattgat ctgttctgat
     7561 gaatactctg catatgtttc tacttctttt aatttccgat agattttatt ctctagtgaa
     7621 gtcggctcca cggtgttttc atgctctact tgaaatccta agacagagcc atcttctagc
     7681 gcattcttaa tggtataggt atgcaagacc tctccatact gatcatgggt tgtacgtgcc
     7741 aattgccctt tggcttgttt ccgattctca ggaaaaatcg gtgtacctgt aaagccaaac
     7801 caagtggatt tcggaaacat ttttttgatt tcttccatat tttcagcact taacgctcga
     7861 tgacactcat ctactacaaa cacgatatgt tggcccatta atttttggaa gcgattggtt
     7921 ccttttttct cttcttgttt ctttgcatat ttcaacgccg catctagttt ttgacgggtt
     7981 gtaatgatta caacatttgc attggcatct gccaacaggg tttcacttaa ttctttggca
     8041 cttcccgttc caacgatcaa gctattcgct ttggcgttcc cggaagaaat cccagtatta
     8101 aattccgaag caaattttgt aaactctgtg gtggtttgat tatctaaatc tttgcggtcc
     8161 actaacatga tcgttcgatc gatccccgat tttcgtgcta aaagcttagt agatacaaaa
     8221 ctagtcaacg tctttcctga accagtcgca tgccacacat accctgattg atgtttattt
     8281 gcggcaataa acaaggcttg aatcgcatgt acttgataag gatgtaacac catgagggtt
     8341 ttattatctt gatcttcact gacgattgta taatccgcaa tcaaacgatg agcatccggg
     8401 atatttaaca cttgtttaca aaactcatat aagttttcta ctttttcatt gtctttcgtt
     8461 cgccaactaa ataaaaactt agggtgcaag tctttcggta aggcatttgc aaaataccga
     8521 gtggtttgtt cattggaaac cacaaacaac tgtaaggtcg aaaagatatt gttccgaaac
     8581 ataccttctt ctgcatattt tttgatttgg ttaaatgcct gataaacacc atcttttgca
     8641 gttacttgtt tcagctcgat ttggacaatc ggtaatccat tgattaataa ggtgacgtca
     8701 aaacgtcgat cccgtgcttc tatgttactt ccacgttttg caatttgatg gaccacttca
     8761 taagtagaga ttcctccacc aatgtcttga ttggaataaa ggataagtga gacagaaccc
     8821 aattgcgaat cttcccgttc aatcgtgatc cgagccatcc cattttcacc ttttaaccat
     8881 ttcgcagcat caaatggggt tttcgttcgc agcagtaatt ccgttttgat cgtttcaaat
     8941 tctttatcgg tcaaaggaaa atcattcagt tctgcttgat tatttgagat aattttttgg
     9001 cgtaaattga cccaaaggtc ttcttctgat tttagatccg gacgatacgt ccactgatta
     9061 tgcccttctc caagtacacg aatcaactgt tcttcgactt ctgcttcctc acggtgacta
     9121 attttcattc ttcttccctc ctagacaaac atcttttgta agaagctttt tttcgtttct
     9181 tttagtaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg tttgaagaaa
     9241 gtaccgattt tttgttgttc atcctttaca ggaaccttaa cactgatatt ctcaatatca
     9301 gaactgttat aagaaggcaa tgcccctact tgggagtatc ttggcaaatt tatagtttca
     9361 aacaatattt taccaaaata gatatcccaa gtattattaa ataaataagc catggtattg
     9421 ttatctatta agaaagggct tgtgaccaaa cgttttctat taagcattat tgctgcgcca
     9481 actttagcaa atataatcgc tggcacatct tttattggct tccatttttt acgctcaatt
     9541 tgctcattag atacataatg atttgcattt ctcatttcat actcattacc aatgttattc
     9601 atgtcggaaa ctttaaaaaa tggagtacct tcagaaccac cttgctcggc atcaggaaat
     9661 ccaattcccg attgagtttt acctaattct cccaacttac gctcttccca atcttccgta
     9721 aaaccaggaa accggatttc tggcactttt gctccgtttt cagggaacat tttttgtaag
     9781 aatccttttt tcgtttcctt cagcaaatct aacttacgtt gatgaagagc gatagtgtcg
     9841 tcgagttttt tgaagaatat gccgattttt tgttgttctt ttaaatcatt gggaatatag
     9901 aatactgtat ctgaaacttt tttccagtct gaacgaggca tttttgttcc agatgtatca
     9961 tttgcagcct tttgatatct atcagcttga ataagagaat aaataaagtc cgagtcgctc
    10021 ttcttagctc taaaaaccca gaaatcccca atcgctacgc ctgtaaaagt tgccttgagc
    10081 caatttttta aatatggacg taactttcca tatagaatat catttggtaa aaattcaatc
    10141 ccttttctat tatcaaattt ctggctcaca tctttattca gtctaccttc tcctgccacg
    10201 atatcttcaa attcaacttt tggaagtcta ctggagtttg tacttttatt aagtctaaca
    10261 actaaatcaa ttaacttacg ctcttcccaa tcttccgtaa atcctgggaa acggatttct
    10321 ggtcgtgtat cattgctcat gtttgaacac ctccagcgca ccttgtaacc acatttcgtt
    10381 ttcaggtgtt gtttgcaggg aagagatagc ttctaaaagt tctttttcta gcacttgctg
    10441 ttcttgtcgt atcttcttga tgtcatttcc gacatgaacc atatcaattg gttcttcttc
    10501 ttcgaaagta tctacatagc gtgggatatt taagttatag tcattttctt tgatttcatc
    10561 aaaggttgcc aaatgagcgt atttctcgac atcttttctt tcagcgtagg tttcgaggat
    10621 tttttgaatg ttttcttccg atagtttatt ttgatttttc cctttaacaa attcacgact
    10681 agcatcaatg aataatacat ctcgagtttg acgattcttc ttcaacacaa tcaccgtcgt
    10741 tggaatcgat gtcccaaaga acaagttcgc aggcataccg atcacagcat aaatactgcc
    10801 atcttctagt aatttttgac ggatgacccc ttctgcagcg ccacggaata aaactccgtg
    10861 tggtaagacg atggccatgg tacctgtttc cttcaaatga tagaacccat gaagaagaaa
    10921 cgcaaaatct gctttggatt ttggcgctaa tttcccataa cgattaaacc gagaatcatc
    10981 taagaaagtc gtatctgctg accaattagc cgaataaggt gggttcatta ctactgcatc
    11041 aaatgtgtaa ggttcatctg ttggccagtc cttattcaac gtatccccat tgcggagatt
    11101 catttcttcg gcgtcgacac catgtaaaat caagttcatt tttgctagat tgtatgtcgt
    11161 tgtatttagt tcttgcccat gatatttaac gttatctggg tgtgttaagt agttacgtac
    11221 gtttaacatc aatgaacctg aacccatcgt tggatcaaaa acactaaaga atcgtcgttc
    11281 cttttgatcg agcgttacga tttgcgccat catatcagaa accatatgag gtgtgtaaaa
    11341 ttctccggct ttcttccctg cttctgaagc aaattgactg atcagaaact catacgcatc
    11401 cccgatcacg tccccatcgt gttctaaaac atctacatca ttcaatttct tgatgacttc
    11461 ggtaatcgtc acatttcgtt gttgctcatc tgttcctagt ttttttgatt ggagatccac
    11521 atcatcaaat aacccattaa attgattgta ggtagatgcc aattgcacaa aggctttgtt
    11581 tagatcgttc aattggaacg ttgcttgttt tgcttgatcc gctaagacgt tgaaaagata
    11641 ctctggtgca attgcatatc ccaaaatatc aacgattgtg gcaatcagat cttcttttga
    11701 ttcttcatct gaaagtaatt cacgatacaa cattgtttgt ttgcttactg tgtcatattc
    11761 ctctaaggat tcatctgcta aaagaaccac ttgttccaac aatcgatcgg ataaatattt
    11821 atagaaaatc agtcccaata aataattttt atattctgac gcatccattt tactacggag
    11881 attatctgcc gcactaaata atttttgatt taattctgca cccaaaaaga tacactccta
    11941 tctcttattt aaagttgcta tataccctct tattttagca tacccccttt tataaaaagc
    12001 aaaaaaaaaa gaaagaaact tattcttgct aataaactac gcgaaaggag tctaacgttg
    12061 tttgatataa cttagactga tcgttaactt attttttata tttctacata gttatcttta
    12121 gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa aaacagtact
    12181 gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat cactacctga
    12241 aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt catttcagat
    12301 acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag cttatcctaa
    12361 gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat aaatgaaaaa
    12421 gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca gttacctgtc
    12481 cgttcttaaa taccacagaa taatcttttt acccattttc aagtaaccga agtggaatgt
    12541 ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct aataaatctg
    12601 ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt cctttatctt
    12661 catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat gaatcaattc
    12721 tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat gaatcgattc
    12781 tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg gaaactctct
    12841 tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt cccacgcttt
    12901 tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat ttcacactat
    12961 catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct acataataat
    13021 cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct gatatctcta
    13081 taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc aagctagtag
    13141 tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc tccgcactgt
    13201 tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt tatatattgt
    13261 gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag ataaagcagt
    13321 tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat tttcaaggcc
    13381 gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc atccttctcc
    13441 ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt cacaaatctt
    13501 aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc tagcatagcc
    13561 aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc ttgttcactt
    13621 tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa ctgaacattc
    13681 atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt tgggtaatac
    13741 ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact gcgttcaatg
    13801 gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt aaagtcaaac
    13861 ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc cggagcagcc
    13921 tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa tttaaatact
    13981 ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc aatccctttc
    14041 cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact gttgtttaac
    14101 gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt cttgcgatta
    14161 ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta agattctcat
    14221 cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg gtactaacaa
    14281 catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt aaagctgctt
    14341 gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta attcgtctat
    14401 cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata ggtaaatcat
    14461 agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact acccagccat
    14521 atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct tctcccaaca
    14581 tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg ctggcagcga
    14641 tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag gataaaattg
    14701 cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag ttttcaaaca
    14761 aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc aatgtttccc
    14821 aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata gccgttaaat
    14881 tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga attactctag
    14941 cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa ggcagctctc
    15001 cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc tcattaattg
    15061 tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca tagtccacaa
    15121 aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa taaggtaaat
    15181 cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca tcaattactt
    15241 gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc ataattcgcg
    15301 tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc tgtatatctt
    15361 ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct tctgtatgct
    15421 gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc tctgcctggt
    15481 aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc tgtttgtatt
    15541 gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt taagcttccg
    15601 cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat cactgtatca
    15661 tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg tcctgctgac
    15721 acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt cacggtgtcg
    15781 atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat cgcatgttta
    15841 gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt ttcttctgga
    15901 tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt cacttcagaa
    15961 ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc tattgccgtt
    16021 tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat aaaggttgca
    16081 acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt ttctccaata
    16141 aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa accgctcaag
    16201 ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg ttttttcagc
    16261 ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt gatcccagtg
    16321 gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc aaaggtagca
    16381 cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc agaacccgta
    16441 ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt aaccaccaaa
    16501 aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc gttaatccat
    16561 tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat gacaaccaac
    16621 gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt aataaacgaa
    16681 ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt aaagataatc
    16741 ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa agaaacggca
    16801 tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc aacagtaatc
    16861 gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag tactggcata
    16921 aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact actgtctgat
    16981 agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc acctgttata
    17041 gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga aataatactt
    17101 tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt ttgaatttcc
    17161 ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc gctatccacc
    17221 acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct tttatctttg
    17281 agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc ttttccccca
    17341 acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc tccttaaagc
    17401 agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag tttgtcttgg
    17461 tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc caataatagc
    17521 acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt tgaccgtata
    17581 gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact tactgctaaa
    17641 ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc agcgggaaat
    17701 tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt caacaattct
    17761 gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag cattccaaca
    17821 atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca atagccaatt
    17881 tcaatcattc cgagatataa aggtatcaat agaatgattt gggctagtat tcttaaaaat
    17941 taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt atttcaattc
    18001 caccaattat caataaaaat accaaacata tccataataa ttttttcttt ttcatttctt
    18061 ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta atattttttt
    18121 gtcacctgta ttagtattta atttatagga aactataaaa actccctagt gaatttacac
    18181 ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt aacattaaca
    18241 cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac tcaatattta
    18301 tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta tctccatttt
    18361 catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct tgtaaaatct
    18421 gtgttaccaa tggttttgat gacttctcta atccagatgt acctgtcatt tttgaaaaaa
    18481 taatacctat aatagccaag acaacaatac ctataatgac aaacttcttg ttcatattta
    18541 ctttattcaa ctttttatct cctatctcca aaataatttg ttcttatccc tcttatcagt
    18601 acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct atcatcaaaa
    18661 tcgtacccat tttctcatct ttttctcgat ataagtagcc cataataaca gcaacagcac
    18721 caaatacaac aggaataaat gctaacgata ctaccataga aatccaacca ataatcaaat
    18781 acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta attgtctgct
    18841 cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt agtgctccac
    18901 aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta caatacatgt
    18961 cttctcctcc taaaatcatt caattgctta ttagttaact gtttacttta ttaaatacat
    19021 cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc atttgatagg
    19081 gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta acattcattt
    19141 tttttgctaa ctccgtttga gaaagtcctt tttcttttct gtatcggctg agattattta
    19201 cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg tatttcaact
    19261 cataaaatat aaaaaacttc aaataccatt acaaatacaa caacttgttg tactatactg
    19321 agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag aaaagcgaaa
    19381 agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac aacaggcagt
    19441 agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaacag ctctttctag
    19501 agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt atgaggaaga
    19561 gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa ttgactatat
    19621 gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa
    19681 taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat
    19741 aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt ccatcatctc
    19801 ttgttacact tgtaaattga tacagaggaa ctatcccagc tcctgataat atcaattctt
    19861 ttattgtttc atctatccct gcccctttcg ctaaatatat taaaaaacta ctacttgctt
    19921 tcacattgta tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa
    19981 caaagtagcc tctgtttttt attaaagcat ttttcacgct gttatcccca acattacgta
    20041 ggcgttcaca aacagtgtta ataagttctt tctctgtcat tagatatgtc ccttgccacg
    20101 ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctgagtt
    20161 ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaa
//
LOCUS       DAFCLG010000042        19886 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFCLG010000042 DAFCLG010000000
VERSION     DAFCLG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.042"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(369..995)
                     /locus_tag="LND56_001396"
     CDS             complement(369..995)
                     /locus_tag="LND56_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743328.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="LND56_001397"
     CDS             complement(1290..1625)
                     /locus_tag="LND56_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6743329.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="LND56_001398"
     CDS             complement(1612..1896)
                     /locus_tag="LND56_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK6743330.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="LND56_001399"
     CDS             complement(1952..3475)
                     /locus_tag="LND56_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6743331.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="LND56_001400"
     CDS             complement(3468..4643)
                     /locus_tag="LND56_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6743332.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="LND56_001401"
     CDS             complement(4647..4832)
                     /locus_tag="LND56_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743333.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="LND56_001402"
     CDS             complement(4798..6492)
                     /locus_tag="LND56_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6743334.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="LND56_001403"
     CDS             complement(6489..6962)
                     /locus_tag="LND56_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBK6743335.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="LND56_001404"
     CDS             complement(7031..7186)
                     /locus_tag="LND56_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBK6743336.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="LND56_001405"
     CDS             complement(7320..7700)
                     /locus_tag="LND56_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6743337.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="LND56_001406"
     CDS             complement(7704..7919)
                     /locus_tag="LND56_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743338.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="LND56_001407"
     CDS             complement(7922..8329)
                     /locus_tag="LND56_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBK6743339.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="LND56_001408"
     CDS             complement(8595..10070)
                     /locus_tag="LND56_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBK6743340.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="LND56_001409"
     CDS             complement(10060..10908)
                     /locus_tag="LND56_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBK6743341.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="LND56_001410"
     CDS             complement(10945..11307)
                     /locus_tag="LND56_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743342.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="LND56_001411"
     CDS             complement(11308..11466)
                     /locus_tag="LND56_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743343.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="LND56_001412"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="LND56_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="LND56_001413"
     CDS             complement(12228..12539)
                     /locus_tag="LND56_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743344.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="LND56_001414"
     CDS             complement(12583..12876)
                     /locus_tag="LND56_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743345.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="LND56_001415"
     CDS             13070..13717
                     /locus_tag="LND56_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743346.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="LND56_001416"
     CDS             13778..14923
                     /locus_tag="LND56_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6743347.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15362..16180)
                     /locus_tag="LND56_001417"
     CDS             complement(15362..16180)
                     /locus_tag="LND56_001417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743348.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(16280..17605)
                     /locus_tag="LND56_001418"
     CDS             complement(16280..17605)
                     /locus_tag="LND56_001418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743349.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(17774..18703)
                     /locus_tag="LND56_001419"
     CDS             complement(17774..18703)
                     /locus_tag="LND56_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743350.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIH
                     QQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVIT
                     EIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRK
                     DNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
     gene            complement(18951..19301)
                     /locus_tag="LND56_001420"
     CDS             complement(18951..19301)
                     /locus_tag="LND56_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743351.1"
                     /translation="MIFENESEEIGRMAEENKVVPFETLVINKEEFEQLFLDFFIQYD
                     RIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
     gene            complement(19342..19836)
                     /locus_tag="LND56_001421"
     CDS             complement(19342..19836)
                     /locus_tag="LND56_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743352.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
BASE COUNT         5860 a         3951 c         2975 g         7100 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaagtt aagctcatta aagataattg acgatagttg
    15061 gtatttcaac gatttttgaa tagaactaat cactttatta agtctaattc accttaagtg
    15121 gttttttagt cgtattcaaa aacgtattca agttgcgtgg cgtgcttttg ataagaaatc
    15181 gtaagaacta gttaatcaaa cgacttataa ataatgtaac accactgatt aatcagctat
    15241 atccaaaaaa acaagcattc attcagatac atcaatctct atagaataca aagctttcat
    15301 tatctcaaac cattaccaca acttggcaag tcggttaatt gaagaacacc tcattctcca
    15361 cttatagcag tggagcaaca agttgagcta ttgttgcaac agaagctaag aattctgtac
    15421 tccatttaag tttttttaga ctatctaccg caaacctcag catagttttt ttaggctttt
    15481 ctttaatagc ttcatcttta atggtttcga tatactcctg agcctcttct acctcttctt
    15541 caggtaagga atcattcaaa ttctccaaaa gcaaatcaag ctttgataat aattccgaca
    15601 tatcatcaat agaaacaatc atattattat tgtggccttg ctgtatatta gtgttgtaac
    15661 tatttccaaa tatatttact ttattatata ttgaattgtt gctcatatag tcctccagtc
    15721 gtcctgctgc attacttgca tcttgtaacg tcacaaaaaa atctctccca tgttggtaaa
    15781 ttgattcaac gatttcaaat tctggatttc catattcgcg ccattcgtat tcttccttaa
    15841 agtattttcc gtaactatgg ttaaattgca cagaattttt accaattttc cttagaagtt
    15901 ttatctctct ctcagaaata aaattttcct caaaagctga ttcaactatc tgaaaatcac
    15961 taagaaatgt tcctaaacag aagattgatt cattaatagt ttgttgaaaa ttccatggta
    16021 ttttgttcca aaaaccattc ttaattaaag cctctttttt ttcaacaatc tgtgatttta
    16081 cattttctgt atatatcttc caattattga cgtcttcctt aatgtccgaa atcgattgac
    16141 cttcataaga agtccctcca ccaaaaagtc cttgaaccat tttatctcat cctctctata
    16201 ataattactc taaatccaat acttgctaca aatgatagca tttcgtgtgg taacatgatt
    16261 gccatcgttc ctgttttatt tactctgact tcaatacttg gtttgttgat gaaattttac
    16321 caattgattc catcgatcgt ttcatggaat ctgtatttac tattttcatt gattgtagag
    16381 attgatttat tccctctgtt gcacttctga tacctttagt atcaaagttt tgcatgattg
    16441 ctcgctgagc tgataaagct gataatgttt gacctattga ctctatcgac ctcttcatgg
    16501 aatctgtatt taatattttt attgattgta gagattgatt tattccctct gttgcccttc
    16561 tgataccttc agtatcaaag ttctgcataa tttcttgctg gactgataat gtttgaccta
    16621 ttgactccat cgactttttc atggaatctg tatttaatac tttcattgct gatagagact
    16681 ctgacaattt agtaacacct gctaaatcga tattactaaa ataatttcca atatatcctt
    16741 caaaatcatc tgacatatca ctaaggtcct caccagcaat atctccagcc aatatttttt
    16801 gaaaagcttt ctctagttga tttgtatatc tcttcaattt ctttattaat ttttggtata
    16861 aagccatttc tatttctttg ttatggctta caatattcct accttctctg attaaactca
    16921 tgtctgtttg aatttcttct agcttaacat ccatatattt actaaatact tcactccaca
    16981 aagaatatga atcagatata gtgtgactgt tatcttgaaa taacttcaat aattgatcct
    17041 gagatatttc tttatacctt acaatattct cttcattttc ttgtgtaact aaaatataat
    17101 tttctccaaa gagatattct tcaaaatcat ataaatctat ccattgtaaa agattctttg
    17161 agtcaaactt tcctattgct tgtatttgtt ttctttttat tgacgaaatg atttcatttg
    17221 gaattgattc ctctgcccag ttatcagaaa aatatgccgg agctaataaa tagattaatt
    17281 ttcttaatgc ccattcgtaa ctatgcaaat acggtaatag ttttttcgaa aaaaacaacg
    17341 catttgtatc taaaagggta gtgtagttta attttgattt acttgcttca atattgacct
    17401 tagctaggaa aatttgaaat atatgtacct cttttattcc tatcttacca gtcttattat
    17461 attgaaatac tacgtgtaac gaatcattat agttctttat gtcaaagcca aatttataat
    17521 ttttttcctg ctcctgaaat cttaagatag aatcactaat atatggtgac aacactttca
    17581 gaaaggtttc ttcgatattt tccattcttc cttttttagg ataatataaa tattcaacaa
    17641 cttgcttact aatatgtcat caacttcttt cctttcaaca attttaattt tcctattaac
    17701 ttgatatttg ctacataagc cacaactagc aaacagaaag agaacttttt ctttaccttg
    17761 ataactgtgt taattactgg tttctttttt ctatttcttt atagtattgt tcttctactt
    17821 ttaatatttt tttaacttct ctttttatgt cagcatacat agatatactg ttgtcctttc
    17881 ggttgaagca gtgataattc tcaccatcaa actcataaaa aagcatatca cttgagcttc
    17941 catctgaatt aattaactga aatttggtat tgagtagtaa ttcatttttc tcgtcattca
    18001 actttattaa aataggtttg atctctttta aataattgaa atatatttcg gttattacta
    18061 aattaaattt cgctactgta taaacataag agatacgtgg ggaatctcta aattggttat
    18121 atatatcttt ccttaaaaca tctaactcct tttttaattg tccgttaaaa tcaaaatgag
    18181 gcattgctag aatttcatcc aaatcaaagt acactctttg ttgatgaata tttactggca
    18241 gatgaccatg ctgagaataa tttctcatat gtaataaaaa cctatacgaa aaatgctcat
    18301 cataaggctt actcagaatt ttattcttaa aagatttaaa atctttttct gctaaaaagt
    18361 ttttcataca aacttcaatg gattcagtca aagttttggc tgaactaatt aagtttatcg
    18421 tcaaagcatt tatctgataa taatactggt cttccgttaa tttttcacct gtactttcaa
    18481 ttaaatcatt agtaaaaatt gtgaagtgtt tgagcaaatt agttaaatta tatctaaaca
    18541 cattaaaaag tttatccaac tctataatat gacgtgttaa ttctacataa tttaaaaata
    18601 ttcttttatc agattctgtt attgtttcta ataatgaatc aatctgcctt attagctcgt
    18661 catcttgtaa attactgaca aaaaacctac tatcacttct cataattata ttctcctaat
    18721 atgatttttt caaaataatc aatatgtaac atagaagtat actctattgt tacatacctt
    18781 atttcttatt atatcagtat ttctctgtga cacataaggg tgtaacataa ttattttaaa
    18841 cgaaatgact acaagtagtc attgaagcaa atctccataa gacatttgta aaatctttta
    18901 aaaagaagcg tcgaaagaag gaacttttct tcaacgcttc ttttattgac tcaattttca
    18961 atattacttg aacgaataat ctgatttcta aactctcgaa aaacttcttg tggttgataa
    19021 tagtctggat ctatttcaaa tgtttctgag aataattcat tgaatttttc tactgaaata
    19081 atgattttac cattcttctc tcgactgttt tctattattt ctgcttctaa ttcaaataaa
    19141 tctagtgacg aactaaaatc ataaatcctg tcatattgaa taaaaaaatc aagaaaaagt
    19201 tgctcaaact cttccttatt tatcactaat gtttcaaacg gaacaacttt attttcttca
    19261 gccattcttc caatttcctc actttcattt tcaaaaatca caaaaatgat attaacttaa
    19321 aagccactaa caaaataaga cttacaaagt aaagggtaat ccaacaaaat aaccattcgc
    19381 tctacgtact acgatttggt agtcctgcct ttcaatcggt aattcataat agtaatttac
    19441 tgtttcttct ttgtcacttt caatatactc tggattatct aatatcatag catactcata
    19501 aagctcttca ccatccttat tttcaatcga ccattgttgt ggacgtattg gtttttctgt
    19561 atcattttta agtgtatagg tcacttgtaa tacacgatca tgttgtgcaa attcatttct
    19621 tttttcggtc acttcaatat ttttaaatgt tagttcaacc ccatctgcct gataagtttc
    19681 acctattttt aaatctattg ttacaatttt tattggcttt tcttctggaa aatgtctggc
    19741 tttaattgtt agataacttc caaaaagaat caatcctagt agcactaaaa gaattccttt
    19801 aacgagccac tgaaacggga agtaaacttt aaacatcttt tttctcctcc gttttgtatt
    19861 tgttttttag agaatacgat aacttt
//
LOCUS       DAFCLG010000043        19237 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFCLG010000043 DAFCLG010000000
VERSION     DAFCLG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.043"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..634)
                     /locus_tag="LND56_001422"
     CDS             complement(38..634)
                     /locus_tag="LND56_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6743353.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="LND56_001423"
     CDS             984..1328
                     /locus_tag="LND56_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK6743354.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="LND56_001424"
     CDS             1329..1748
                     /locus_tag="LND56_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBK6743355.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="LND56_001425"
     CDS             complement(1840..2190)
                     /locus_tag="LND56_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743356.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="LND56_001426"
     CDS             complement(2156..3487)
                     /locus_tag="LND56_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBK6743357.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="LND56_001427"
     CDS             3742..4308
                     /locus_tag="LND56_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6743358.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="LND56_001428"
     tRNA            4453..4540
                     /locus_tag="LND56_001428"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /gene="bsh"
                     /locus_tag="LND56_001429"
     CDS             4876..5850
                     /gene="bsh"
                     /locus_tag="LND56_001429"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK6743359.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6175..8070
                     /gene="asnB"
                     /locus_tag="LND56_001430"
     CDS             6175..8070
                     /gene="asnB"
                     /locus_tag="LND56_001430"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBK6743360.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8265..8711)
                     /locus_tag="LND56_001431"
     CDS             complement(8265..8711)
                     /locus_tag="LND56_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK6743361.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8939..9088
                     /gene="rpmG"
                     /locus_tag="LND56_001432"
     CDS             8939..9088
                     /gene="rpmG"
                     /locus_tag="LND56_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6743362.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9176..9694
                     /locus_tag="LND56_001433"
     CDS             9176..9694
                     /locus_tag="LND56_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6743363.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9771..10727)
                     /locus_tag="LND56_001434"
     CDS             complement(9771..10727)
                     /locus_tag="LND56_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6743364.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10893..11699
                     /locus_tag="LND56_001435"
     CDS             10893..11699
                     /locus_tag="LND56_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743365.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11696..12685
                     /locus_tag="LND56_001436"
     CDS             11696..12685
                     /locus_tag="LND56_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6743366.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12682..13686
                     /locus_tag="LND56_001437"
     CDS             12682..13686
                     /locus_tag="LND56_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6743367.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13818..14360
                     /locus_tag="LND56_001438"
     CDS             13818..14360
                     /locus_tag="LND56_001438"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK6743368.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14380..15078
                     /locus_tag="LND56_001439"
     CDS             14380..15078
                     /locus_tag="LND56_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK6743369.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15099..15311
                     /locus_tag="LND56_001440"
     CDS             15099..15311
                     /locus_tag="LND56_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBK6743370.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15311..16273
                     /locus_tag="LND56_001441"
     CDS             15311..16273
                     /locus_tag="LND56_001441"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBK6743371.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16291..16686
                     /locus_tag="LND56_001442"
     CDS             16291..16686
                     /locus_tag="LND56_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6743372.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16849..17214
                     /locus_tag="LND56_001443"
     CDS             16849..17214
                     /locus_tag="LND56_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBK6743373.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17211..19022
                     /locus_tag="LND56_001444"
     CDS             17211..19022
                     /locus_tag="LND56_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBK6743374.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19084..19230)
                     /locus_tag="LND56_001445"
     CDS             complement(19084..19230)
                     /locus_tag="LND56_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBK6743375.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
BASE COUNT         6220 a         3278 c         3927 g         5812 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     6121 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     6181 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     6241 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     6301 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     6361 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     6421 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     6481 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     6541 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     6601 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     6661 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     6721 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     6781 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     6841 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     6901 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     6961 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     7021 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     7081 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     7141 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     7201 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     7261 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     7321 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     7381 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     7441 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     7501 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     7561 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     7621 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     7681 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     7741 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     7801 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     7861 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     7921 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     7981 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     8041 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     8101 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     8161 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     8221 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     8281 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     8341 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     8401 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     8461 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     8521 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     8581 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     8641 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     8701 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
     8761 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     8821 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     8881 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     8941 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     9001 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     9061 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     9121 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     9181 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     9241 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     9301 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     9361 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     9421 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     9481 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     9541 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     9601 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     9661 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     9721 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     9781 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     9841 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     9901 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     9961 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    10021 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    10081 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    10141 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    10201 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    10261 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    10321 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    10381 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    10441 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    10501 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    10561 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    10621 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    10681 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    10741 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    10801 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    10861 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    10921 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    10981 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    11041 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    11101 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
    11161 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    11221 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
    11281 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    11341 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    11401 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    11461 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    11521 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    11581 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    11641 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    11701 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    11761 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    11821 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    11881 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    11941 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    12001 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    12061 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    12121 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    12181 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    12241 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    12301 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    12361 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    12421 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    12481 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    12541 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    12601 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    12661 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    12721 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    12781 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    12841 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    12901 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    12961 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    13021 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    13081 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    13141 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    13201 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    13261 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    13321 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    13381 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    13441 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    13501 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    13561 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
    13621 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    13681 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    13741 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    13801 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    13861 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    13921 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    13981 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    14041 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    14101 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    14161 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    14221 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    14281 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    14341 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    14401 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    14461 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    14521 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    14581 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    14641 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    14701 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    14761 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    14821 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    14881 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    14941 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    15001 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    15061 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    15121 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    15181 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    15241 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    15301 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    15361 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    15421 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    15481 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    15541 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    15601 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    15661 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    15721 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    15781 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    15841 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    15901 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    15961 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    16021 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    16081 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    16141 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    16201 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    16261 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    16321 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    16381 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    16441 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    16501 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    16561 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    16621 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    16681 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    16741 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    16801 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    16861 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    16921 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    16981 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    17041 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    17101 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    17161 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    17221 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    17281 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    17341 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    17401 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    17461 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    17521 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    17581 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    17641 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    17701 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    17761 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    17821 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    17881 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    17941 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    18001 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    18061 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    18121 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    18181 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    18241 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    18301 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    18361 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    18421 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    18481 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    18541 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    18601 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    18661 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    18721 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    18781 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    18841 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    18901 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    18961 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    19021 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    19081 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    19141 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    19201 agcggctgtt gccagacttc ctactaatat tcagata
//
LOCUS       DAFCLG010000044        19059 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFCLG010000044 DAFCLG010000000
VERSION     DAFCLG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.044"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..676
                     /locus_tag="LND56_001446"
     CDS             98..676
                     /locus_tag="LND56_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6743376.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            676..1605
                     /locus_tag="LND56_001447"
     CDS             676..1605
                     /locus_tag="LND56_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743377.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1602..3338
                     /locus_tag="LND56_001448"
     CDS             1602..3338
                     /locus_tag="LND56_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6743378.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3401..4237)
                     /gene="pheA"
                     /locus_tag="LND56_001449"
     CDS             complement(3401..4237)
                     /gene="pheA"
                     /locus_tag="LND56_001449"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBK6743379.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4234..4743)
                     /locus_tag="LND56_001450"
     CDS             complement(4234..4743)
                     /locus_tag="LND56_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBK6743380.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4736..6031)
                     /gene="aroA"
                     /locus_tag="LND56_001451"
     CDS             complement(4736..6031)
                     /gene="aroA"
                     /locus_tag="LND56_001451"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK6743381.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6044..7135)
                     /locus_tag="LND56_001452"
     CDS             complement(6044..7135)
                     /locus_tag="LND56_001452"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBK6743382.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7152..8318)
                     /gene="aroC"
                     /locus_tag="LND56_001453"
     CDS             complement(7152..8318)
                     /gene="aroC"
                     /locus_tag="LND56_001453"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK6743383.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8320..9384)
                     /gene="aroB"
                     /locus_tag="LND56_001454"
     CDS             complement(8320..9384)
                     /gene="aroB"
                     /locus_tag="LND56_001454"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK6743384.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9417..10439)
                     /gene="aroF"
                     /locus_tag="LND56_001455"
     CDS             complement(9417..10439)
                     /gene="aroF"
                     /locus_tag="LND56_001455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBK6743385.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11098..12072)
                     /locus_tag="LND56_001456"
     CDS             complement(11098..12072)
                     /locus_tag="LND56_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBK6743386.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12053..13936)
                     /locus_tag="LND56_001457"
     CDS             complement(12053..13936)
                     /locus_tag="LND56_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBK6743387.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13954..16179)
                     /locus_tag="LND56_001458"
     CDS             complement(13954..16179)
                     /locus_tag="LND56_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6743388.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16294..17328
                     /locus_tag="LND56_001459"
     CDS             16294..17328
                     /locus_tag="LND56_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6743389.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17395..18408)
                     /locus_tag="LND56_001460"
     CDS             complement(17395..18408)
                     /locus_tag="LND56_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6743390.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18486..18674)
                     /locus_tag="LND56_001461"
     CDS             complement(18486..18674)
                     /locus_tag="LND56_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK6743391.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         5351 a         4114 c         3485 g         6109 t
ORIGIN      
        1 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
       61 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
      121 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
      181 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
      241 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
      301 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
      361 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
      421 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
      481 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
      541 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
      601 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
      661 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
      721 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
      781 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
      841 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
      901 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
      961 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     1021 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     1081 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     1141 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     1201 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     1261 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     1321 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     1381 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     1441 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     1501 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     1561 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     1621 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     1681 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     1741 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     1801 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     1861 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     1921 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     1981 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     2041 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     2101 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     2161 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     2221 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     2281 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     2341 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     2401 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     2461 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     2521 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     2581 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     2641 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     2701 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     2761 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     2821 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     2881 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     2941 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     3001 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     3061 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     3121 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     3181 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     3241 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     3301 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     3361 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     3421 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     3481 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     3541 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     3601 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     3661 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     3721 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     3781 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     3841 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     3901 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     3961 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     4021 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     4081 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     4141 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     4201 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     4261 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     4321 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     4381 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     4441 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
     4501 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
     4561 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
     4621 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
     4681 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
     4741 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
     4801 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
     4861 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
     4921 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
     4981 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
     5041 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
     5101 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
     5161 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
     5221 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
     5281 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
     5341 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
     5401 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
     5461 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
     5521 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
     5581 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
     5641 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
     5701 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
     5761 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
     5821 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
     5881 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
     5941 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
     6001 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
     6061 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
     6121 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
     6181 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
     6241 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
     6301 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
     6361 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
     6421 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
     6481 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
     6541 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
     6601 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
     6661 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
     6721 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
     6781 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
     6841 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
     6901 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
     6961 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
     7021 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
     7081 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
     7141 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
     7201 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
     7261 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
     7321 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
     7381 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
     7441 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
     7501 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
     7561 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
     7621 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
     7681 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
     7741 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
     7801 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
     7861 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
     7921 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
     7981 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
     8041 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
     8101 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
     8161 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
     8221 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
     8281 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
     8341 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
     8401 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
     8461 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
     8521 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
     8581 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
     8641 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
     8701 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
     8761 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
     8821 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
     8881 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
     8941 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
     9001 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
     9061 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
     9121 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
     9181 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
     9241 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
     9301 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
     9361 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
     9421 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
     9481 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
     9541 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
     9601 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
     9661 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
     9721 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
     9781 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
     9841 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
     9901 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
     9961 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    10021 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    10081 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    10141 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    10201 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    10261 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    10321 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    10381 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    10441 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    10501 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    10561 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    10621 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    10681 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    10741 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    10801 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    10861 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    10921 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    10981 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    11041 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    11101 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    11161 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    11221 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    11281 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    11341 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    11401 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    11461 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    11521 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    11581 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    11641 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    11701 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    11761 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    11821 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    11881 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    11941 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    12001 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    12061 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    12121 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    12181 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    12241 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    12301 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    12361 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    12421 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    12481 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    12541 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    12601 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    12661 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    12721 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    12781 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    12841 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    12901 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    12961 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    13021 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    13081 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    13141 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    13201 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    13261 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    13321 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    13381 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    13441 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    13501 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    13561 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    13621 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    13681 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    13741 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    13801 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    13861 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    13921 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    13981 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    14041 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    14101 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    14161 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    14221 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    14281 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    14341 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    14401 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    14461 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    14521 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    14581 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    14641 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    14701 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    14761 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    14821 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    14881 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    14941 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    15001 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    15061 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    15121 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    15181 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    15241 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    15301 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    15361 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    15421 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    15481 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    15541 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    15601 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    15661 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    15721 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    15781 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    15841 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    15901 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    15961 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    16021 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    16081 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    16141 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    16201 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    16261 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    16321 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    16381 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    16441 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    16501 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    16561 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    16621 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    16681 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    16741 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    16801 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    16861 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    16921 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    16981 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    17041 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    17101 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    17161 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    17221 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    17281 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    17341 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    17401 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    17461 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    17521 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    17581 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    17641 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    17701 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    17761 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    17821 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    17881 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    17941 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    18001 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    18061 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    18121 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    18181 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    18241 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    18301 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    18361 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    18421 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    18481 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    18541 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    18601 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    18661 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    18721 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    18781 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    18841 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    18901 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    18961 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    19021 cttattggca aatcgccaaa ataatgtaga ctaactaaa
//
LOCUS       DAFCLG010000045        18639 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFCLG010000045 DAFCLG010000000
VERSION     DAFCLG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.045"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..995
                     /locus_tag="LND56_001462"
     CDS             99..995
                     /locus_tag="LND56_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK6743392.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1145..2086)
                     /locus_tag="LND56_001463"
     CDS             complement(1145..2086)
                     /locus_tag="LND56_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6743393.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2212..3333
                     /locus_tag="LND56_001464"
     CDS             2212..3333
                     /locus_tag="LND56_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6743394.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3591..5093
                     /gene="eat(A)"
                     /locus_tag="LND56_001465"
     CDS             3591..5093
                     /gene="eat(A)"
                     /locus_tag="LND56_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6743395.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5121..5687
                     /locus_tag="LND56_001466"
     CDS             5121..5687
                     /locus_tag="LND56_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6743396.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5898..6959
                     /locus_tag="LND56_001467"
     CDS             5898..6959
                     /locus_tag="LND56_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6743397.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6962..7858
                     /gene="murQ"
                     /locus_tag="LND56_001468"
     CDS             6962..7858
                     /gene="murQ"
                     /locus_tag="LND56_001468"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK6743398.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7872..9854
                     /locus_tag="LND56_001469"
     CDS             7872..9854
                     /locus_tag="LND56_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6743399.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9919..10365)
                     /gene="nrdI"
                     /locus_tag="LND56_001470"
     CDS             complement(9919..10365)
                     /gene="nrdI"
                     /locus_tag="LND56_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK6743400.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10576..12000
                     /locus_tag="LND56_001471"
     CDS             10576..12000
                     /locus_tag="LND56_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6743401.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12181..12954)
                     /locus_tag="LND56_001472"
     CDS             complement(12181..12954)
                     /locus_tag="LND56_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6743402.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12951..13856)
                     /locus_tag="LND56_001473"
     CDS             complement(12951..13856)
                     /locus_tag="LND56_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6743403.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14010..14330)
                     /locus_tag="LND56_001474"
     CDS             complement(14010..14330)
                     /locus_tag="LND56_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK6743404.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14804..15490
                     /locus_tag="LND56_001475"
     CDS             14804..15490
                     /locus_tag="LND56_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK6743405.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15492..16436
                     /locus_tag="LND56_001476"
     CDS             15492..16436
                     /locus_tag="LND56_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK6743406.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16635..17279
                     /gene="pcp"
                     /locus_tag="LND56_001477"
     CDS             16635..17279
                     /gene="pcp"
                     /locus_tag="LND56_001477"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK6743407.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17358..18635
                     /locus_tag="LND56_001478"
     CDS             17358..18635
                     /locus_tag="LND56_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743408.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5971 a         3294 c         3971 g         5403 t
ORIGIN      
        1 ttaaattttc atacaaatat aggaaacgtt ttactggctt tttactattt aaacaggcta
       61 tcataggaaa aagtgacaga gaaagtgagg cggaaaagat gaacaaaaat gacgtactgc
      121 tagttgcaaa aaataaatca ctttattttt tgccttccat ccgctcttta gatgtttggt
      181 ccggaaaaga tttactacag tcggaattca ttaaatcagc tagacaacag ttggcaaatt
      241 atcagctgat cgttttttta gattatggat ttgatatgga gatggctcga cgcgtgcgcc
      301 catttaccaa tgcaaaaatt gtgttatttt tttggaatca cttcaaagca gaacacgagg
      361 cgatgctgaa gcaggcacaa gatgaaccgg caatcgatga gatttatcat tttgattttt
      421 tggaagcaaa agaacttggg ctgaaacata atagttcatt ttattcgaaa catatgaatt
      481 tttcttctag cgaaccaaag acggatctat tttttggtgc aacgaataat gggagaaaag
      541 aacgagcaga aagctataag caggaattta taaaaagaga tgttacgacc aactatttca
      601 ttcttccaca tcgagggaat gaacaacctg gatatcttac ttatgaagag tacctgaaaa
      661 aaacagggga atcgcgcggg attctggaac tgttgcggga gggtcagcaa ggagtgactt
      721 tgcggacatt tgaatccatg tattttcaaa agaaactagt gacggataat caagcgattg
      781 ctcattatca tttttatcgt cctgaaaata tttttttgct tcgagaacgt gatttagatg
      841 aattaccgga atttctgcat acaccattta agccaatcct atcagagcta ttggaatttt
      901 atgatgcaga aaattgggca aaacgattta tcactaacga ttccactatt tatgagcaat
      961 acgaatatga cctctcctct gttgaaaaca aataaaaaac ctacattttg aagagattat
     1021 gtactgaaac aaacaagcac aattcttatg gcatcatgag agttgtgttt tctttttgat
     1081 cttttattgg gcttaaaaaa agagaaccag ctgctgattc agctgattct catcagagag
     1141 ccttttattc tgcgccaagg attccttctt ttgcatggac taattgggtc aaaaaggcac
     1201 aatatggtgt agcaatatca tatttttgtc cttttcgcca aatggcacca ttgatgtagt
     1261 cgatttcagt caaacgatgg tttttgatca ggtcttggta catagatggg taatgcaacc
     1321 cgatgttgtc aggatcataa gtagaagcaa tatgttggta cacttcttct tgatctagta
     1381 tgattccttc ttttgctgcg atagaagcaa attcagatac gattgttcga accatgctct
     1441 ctgccgcttt ttgcgcgcct agttcagcaa tattacaatc caaaatcgtg cataaaccat
     1501 tcaatgttcc atttacacaa gcttttcgcc aaatggaata acgaacgtta tggctataga
     1561 tcggatttaa atttgcttct tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt
     1621 ctggttccaa gttctctagt tcgatttctc catcacctaa caatgtgact tttccgggac
     1681 cagctagacc agctgtccac atcgtgatcc caaataagat atttttcttt ggcacatatt
     1741 tttccagtac atcttcatga cctaatccat ttagtaaaca caaaacataa gtattgtcag
     1801 taataatgga ttggatcgaa caaaacatat catctaattg attggctttt gttaatgcta
     1861 cgatcaagtc cacgtgttcg ttgctttcga tgatttcttc tggtgaatag attggcagtt
     1921 ttgcgaccac ttctttaccg ttaaagtcag caatcaaccc atttgtacga atggcttcaa
     1981 tgtgagcagg ccaacgatcg atcaagagga cctcatttcc gctttgatgt agcatcaatc
     2041 cgattcggct gcccattgct ccggccccag ctattgcgat tttcatgttt tttctctcct
     2101 ttattcaatg agttcccttt tagtatatag aaaaatggaa gaaaatagca ttgttttgtt
     2161 tgaaaaaaat gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt
     2221 ttggcggcaa ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt
     2281 ctaaatggct gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga
     2341 gaaaatactg cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg
     2401 atggatctct tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa
     2461 aacaccgcag tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg
     2521 gatcaaacta tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg
     2581 cttcaagaag tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt
     2641 ctaggattac tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta
     2701 ttatctacta aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg
     2761 attatcgctg cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt
     2821 ggaagacaaa aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag
     2881 attgctcagc aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga
     2941 gcagcaggcg gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc
     3001 gatttagtca gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt
     3061 acaggagaag ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca
     3121 gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag
     3181 ctagaaggac tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt
     3241 ttagaagaag caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca
     3301 cttagcagat ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat
     3361 cgtaatttca aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga
     3421 tgaaagctgc attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg
     3481 attggcttgg ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct
     3541 ttagccagtt ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa
     3601 tcgaaataaa aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag
     3661 caaatctaaa ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa
     3721 agacaacttt actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc
     3781 agcaagaatt tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg
     3841 tgttaaatga tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc
     3901 aaacagatcc tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa
     3961 aagtcctact ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa
     4021 cgaatcattt ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac
     4081 aaggcttcat cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt
     4141 tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac
     4201 agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca
     4261 gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata
     4321 aaacaaagaa acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag
     4381 tgggtgctga tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga
     4441 cccagatcag cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga
     4501 tgaatagcca agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt
     4561 atgaaaatct gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga
     4621 tttcaggtcc taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca
     4681 acggcaaggt tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc
     4741 aaaattatga agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact
     4801 accaagcatt tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca
     4861 agatcgagca gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac
     4921 agccagctga actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag
     4981 aacaattaga acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc
     5041 aaaccttcct ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt
     5101 aatctcaaga acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca
     5161 tgctttatgt aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac
     5221 gggcagatgt gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg
     5281 tagtagattt gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag
     5341 atacagcaca agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg
     5401 tagaaatgga ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag
     5461 cacgtttagc gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac
     5521 ggatcgttcc tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt
     5581 gccataatgc cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt
     5641 tctttacatt catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg
     5701 ttatttaaat aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat
     5761 gacaattctt ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa
     5821 aagtattgaa attttgtttc tttagatata tactctagtc atagaaatga agacggatac
     5881 agaaaaagag ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag
     5941 agaagaaaca gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca
     6001 tgcatatacc agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg
     6061 aagcgaaaaa gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag
     6121 cgggtttttc attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa
     6181 tggattatca tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt
     6241 tgaatgctag cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt
     6301 tctcacagat tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag
     6361 aatggtatca gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg
     6421 taccaggaga tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac
     6481 atcgagggat ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg
     6541 tttacatcgg agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa
     6601 aagaacagac agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat
     6661 tagggaagca tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac
     6721 gttttcgaga gattccgcct atcccagcaa gaaatacagc tacgcgtatg aaagggtcta
     6781 ttactttaga taacgaaaag tatttgcgct atatgggtga gatccaatta accaaatatg
     6841 atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa gagctaccat
     6901 tgatcaacca aatcaaagct ggtatgaact atcaatttat aagaaaagaa ggaagataaa
     6961 aatgagcaaa atcaatttag agaacctaac aacggaacgc agaaaccaag aaacttttgg
     7021 attagatgag atgagtattt ctcaagccgt tcaactaatg aacaaagaag atcagaaagt
     7081 ggccgctgcg gttgaaaaag aactgacaag tatcgaacca gtgattgcta acacgattga
     7141 gtcctttaaa aaaggtggac gtctgattta tatgggagcc ggaacaagtg gaagactagg
     7201 tgtgttagat gctgctgaat gcgtaccgac attcggtgta gaaccggaaa tggtcatagg
     7261 tctaattgct ggtggagaag aagcaatgac tgtagctgtc gaaggggccg aagattctta
     7321 tgagctaggg caagaagatc taatcaagct gaatttgaca aaaaatgata tagttattgg
     7381 tatcgcagct agcggtcgta caccatatgt gatcggtgga ttagattacg caaaatcgat
     7441 cggtgctgtc acaggaacga tttcttgcaa taaacaagcc gaaatcagta aacacgcgga
     7501 tttcccaatc gaagtcgatg caggtccaga atttttgaca ggctctactc gattgaagtc
     7561 aggaacagca caaaaactta tcttgaatat gatttctact atcagtatga ttggtatagg
     7621 taaagtattc aataacttga tggtcgatgt caaaccaaca aatgaaaaac taatcgaacg
     7681 ctcaaaacga atcatcatgc aagcgacaga agctgattac gaaacagctg aaaagtactt
     7741 tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg attttgacaa atagtgataa
     7801 agaggaagct caagcaaaat tagctgaagc aaacggattt gtaaaaggga ccttatgagg
     7861 gggaaaagac catggcaaaa aacgaactga ctttggaaga actggcaaaa caaatttata
     7921 aaggggcagg cggcatgggc aatgtagaaa gcattgtcca ctgcatgact cgtgtgcgta
     7981 tgaagatcat caatgatgat cttgtaaatg tctctgaatt aaaagcgatt ccaggagtgc
     8041 ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg acctgggaaa gtgaacaaag
     8101 tagcacagca aatggttgat ttggcaggcg tgaaattagg agaaaaactt tcttctgtaa
     8161 gtacagctac agcaaattca tcagcatctg gaaaagaccg tgtaaacgaa cgggcacaag
     8221 aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa aatcaaatcg gtattaaaag
     8281 acatttctaa tatctttgtg ccaatgattc ctgcatttgt cggtactggg atcgtggcag
     8341 ggattgccgc agttttagca aacttagtca cagccggaac acttgatgca gcaacatggc
     8401 aacaatatat cgatgtgatg aacatcttga aaaacgggat gtttagctac ctagttatct
     8461 atacaggtat caatgctgca caagtttttg gtgcaacacc aactcttggt ggagttatcg
     8521 gggccgttgt gatgctgaca gggatgaatc cggaagcacc actgaaaaac ttatttactg
     8581 gtgaagctct tgcagctgga caaggtggga tcctaggtgt catcttcgct gtttggctat
     8641 tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga tgcggtagat atcatcgtta
     8701 caccaactct ttcattgatt gccatcggtc tgattgaaat tttcttcatc atgcctttag
     8761 caggtttcat ttctgatggc atggtcggtg ggatcaactg ggtacttggc gttggtggag
     8821 catttgccgg cttcgttcta ggcacattat tcttgccgat ggttatgttc ggactgcatc
     8881 aaattttgac tccaatccat gtgcaaatga ttgatgaaac tggtcgtacg ctattgcttc
     8941 caattcttgc gatggctggt gcaggacaag tgggtgcagc attggcatta tggattcgct
     9001 gcaaaaaaga taaagaatta acagaaatga tcaaaggagc tttaccagtc gggatcctag
     9061 gaatcggcga accattgatt tacggagtaa cattacctct aggacgccca tttattactg
     9121 cttgtatcgg tggaggtatc ggcggggcag tgatcggaat gatcggaaat gtcggggcta
     9181 tcgctattgg accttcagga gccgctttga tcccattgat ctctgacggt aaatggttag
     9241 gctacatcct tggattattg gcagcttatg ctggtggctt cgttgctaca ttcttcttcg
     9301 gtattcctaa agaccgttta gaaaaagaag aattagcgac tgaaacagcc acagtaaatg
     9361 aagtgcctga aaaagtagta cctcaaactt cagctgaaag tgtgactatc ctctcatcag
     9421 ttgctgacgg aacagttgtt tctttagata aagcaagtga tccagttttt gcacaaaaga
     9481 tgatgggtga aggctacttt gtagaaccag aaaacggaca tatctattct ccagttactg
     9541 gggtagtcag ctctgtcttc ccaacgaaac acgctattgg gatcacaacg ccaaatggtt
     9601 tagaaatctt gcttcatatg ggaatcaaca cagttgactt aggcggtacg ccatttgatg
     9661 tgaaagtaac agaagggcag caagtgacac ctgatacgct tgtagcagaa gctgacttag
     9721 aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt cttggtaacc aatatggatc
     9781 gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa agcaaaagca cctgtgatgg
     9841 atgtcaaagc ttaatatgat agaaacaata gaaacgaaag aagaaactga aatctatttt
     9901 tcagcttctt cttttttatt atttattgtt ttctgctaaa atctgataaa ttcgtgtcac
     9961 atcagatggt gttccccgta attcataatc tgccaagaaa ggaaagccaa atgtttgtgc
    10021 atattgtttg gctgttagac aatactgatt attgaagttt ttatttccgc taccgactac
    10081 accaagacaa agtttatgat tgtcttcatg ttccagatat tcacgcatcg tttcggtcaa
    10141 gatttctgta tcaccgttat ctaaaccatt tccgccgtct aaataagtag gaacgaaagt
    10201 aaaaaatggt tctgtttctt tcgtaaaatc actattttca tggatttctt ttaaagtaac
    10261 ttcaggattg acaggatctt ctgcatgcat tttttcagca tattctgcta gatgtttggc
    10321 aaaggcacgc gtatttcctg aaatcgatat atataaaatg ttcatggatc atcctccaat
    10381 tctttccttt tagtatagca tcttttttaa gttttttggt ggtgtaattt ggaaagggac
    10441 taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa ttatatttct attaaagttt
    10501 ttattaatta agtaaaaata aaaaaggttt gttatacttt ttgataaaag cggttttatt
    10561 ttggaggatg cttacatgga tacagagcaa cagaaaaaag gaattggttt aattccactt
    10621 gctgccttag taattggttc agcgattgga ggtggcgtat ttggtattat gacggacatc
    10681 tccggatcgg ctggcggccc agctattttc agttgggtac tcgtaggaac agcaatgttg
    10741 atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc cagatttaaa aggcgggatc
    10801 tttagttacg cagaagaagg ttttggccgt ttcgctggtt ttatcagcgg ttggggttac
    10861 tggctcacat gctggttagg caacgtagct tttgcaacat tgttgatgag cgcattagga
    10921 tatttcttcc cagtatttga aggtgggcaa aatctggcat cgatcgtgat gagtacagtc
    10981 tttttatggg gatatgcatg gctagtgaat cggggagtcg aaaatgctag tttcgtcaat
    11041 gctatcgtca cattctgtaa attagtgcca ttatttttat ttattgtagt agctatcagc
    11101 acgttttcga tccaacaatt cattgataac ttttctagcg acattatatt gaatgcgaat
    11161 ggacagccga tcccgttttc cagtcaagta ttgagttgca tcatggtgat gctttgggta
    11221 tttgttggta ttgaaggagc ctctgtcgta ggttcacgag caaaaaaaca atcagatgtt
    11281 gggaaagcaa cgattctagg gatattcggc ttgattatta tttacgtctt ggtttccatg
    11341 cttccatatg ggacaatgac gatgaatcag ctgcaaacga tcaaggcgca gcctgctatg
    11401 ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag tcgtgatcaa cggcggtttg
    11461 atcatttctt tagttggtgt ttggctctca tggacgatct taccgattga aacaatggga
    11521 aacatggctg aagacggact tttgcctaaa gcatggggaa aactcaacaa aaatggggca
    11581 ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg tctttttact ttcattatta
    11641 ttcactgaca gtgcttataa ctttgcttac acacttggta cagcagctat tttcttcacg
    11701 tggcttttct taggattgta ccaaatgaaa ctttcttatc agcggaaaga atgggtccaa
    11761 tttttagtag ggcttttagc aagtgccttt caagtatggg ctatgctagt cgtagccttt
    11821 aatgaagtaa tgcttgttct tgttcttttc ttgccaggca tttattttta cctcaaagca
    11881 cgtaaggagc aaaacgagcc tgtacaacat atccacttag ataagatttt acttataggt
    11941 gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca ctgggatgct tcacgtatag
    12001 aagtataaaa gaagtataaa gagaggttgt gagcctgtcg tttattcaaa aacatactag
    12061 gaaaggagct gtgacacaaa tgttgtcaca gctccttttt cagaataaac ggtgtttaag
    12121 agacagccaa tcggcaataa ggctgaacgt ctctttcaca gcctcttttt tgatgagtca
    12181 tcatacattt tttcgcttaa atacgagata tcctaatgct aagaacaaaa cgatgtaagc
    12241 taaattaccg aatagcattt gattagtaga aagatgagtc aattgcttca tgatctcttc
    12301 gtttccaatt tggttttgca gattcaacat actgatcgga ttccatttga tccactccca
    12361 tttttggatg agtgcaataa ggataccaga tatgattgac gaagcaaaat aaaagacgat
    12421 tcctgctgag atggatgcgc cggaactatt gataaagcaa gcaatcatca acactaaact
    12481 taaaatcagc cataaaccga tataactacc caaagtataa gtgaacaagc tttggatcag
    12541 tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa gggaaaagaa tcagtttcat
    12601 caacacagtg acaataatag ctaaaaggta taaataaaca gaataaataa ctaatgtgat
    12661 ccatttgctt actaatattt ctcctcgaga atactttcga taaaggagat ttttcaatgt
    12721 accatattgt gcttccatcg aaatgatcgt gcttgcagct gcaatcatga taaagacaat
    12781 ccaagatgtg gcactaaaaa gctgagcagt catttcaact ggatcaatga tcgtcgtgta
    12841 ttttttggct agaagtgctg ttcctatgag caaaacaact aatagaacag ataaaacagc
    12901 agttgacttt ttcttgatca atttaaataa ttcttgtttt acaagcaatg tcatttttct
    12961 tcttccccct ttactgaatc cagaatttcc aatagagaag attccaaatc attcgtcaga
    13021 tgctggacgt ctgtcacatg gatattttgt gcagacaata gctggatcac ggttcctaat
    13081 aattcttcgt tttcttttaa aagcttgatt ttatcaggat cttccaaagt atatccagct
    13141 tctgtcaaga tcgttcgagc agctagatca tttgttgtag agataacgat gaaatcttgc
    13201 gctgtttggt tcaattcagc cattgtacat tgtttgatga tctttccttg attgatcagg
    13261 atcagctcat cgaccatttt ttcaagctcg ctgagtaagt gactggagat caagaaagag
    13321 gtgccctgag tagccaagtc ggtaatgatt tttcgcacat ctcttgttgc ttgtggatcc
    13381 aaaccgttca tcggctcatc taaaataacg atagacggat ggttcagtaa ggcaagagca
    13441 attcccaatt tttgtttcat tcccaacgag taggatttcg cttttttgtg aatgtacttt
    13501 tccatcttga gctgattcac gatagtgtcg atagcagata catcgttggt ttcagagaat
    13561 aacttcagat gatcatagcc tgttaagaat ggatagatac ccgggtattc gatcaatgct
    13621 cctacttgtt ccaacccttt atgactagag ggagaaatct cattttcacc aatacagatt
    13681 gttccatctg agtaatggat cagtcctaaa atagctttca tgatcgttgt ttttccagcg
    13741 ccattaggac ctactaatcc aacgattttg ccaggggata agctaaaact gacatcgctg
    13801 agggcttgtt tgtgaccaaa gttcttggat aattcttgga ccgaaagact tttcaaaaat
    13861 attcactcct tttctataga tagtacaagc atacacctga tgagtggaga agtcgctagg
    13921 tcattggact tatttcttat tttttcataa aaaaattgaa tattaaataa aaaggcttat
    13981 tttttctgat cagtacttat tccaagtgtc tagtggccgc tagtaatctt gatgccaatg
    14041 atactgatga gtagcatccc cacaaagaac caagtggaaa gattcagctt atcatgaaac
    14101 aagaccacac caattatgac cgaaccaact gcgccaatcc ctgtccagat aggataagag
    14161 agactcatcg gcaaactttt gattgccttt gacaaaaagt aaaaactagc aatcatgcca
    14221 atcactgtgt aaaaagaata attgatttta gaaaaaccat cactccattt catcatcgtt
    14281 gaccagaata cttccaaaat tcctgctatg attaattcca tccatgccat acttacattc
    14341 ctccttaact ataaaaaaat acacaaaaag aggaaaatca tcccttcaaa gacctaatgg
    14401 aatgatcatc ctctttatcc tacccggtgg cagattgtat atctttttct gtagtatagc
    14461 acacctatcc agatatcggc aatctcatac tttttagtaa agtgtaccaa gcactgacgg
    14521 atatatcgtt agcggaaaag aagatgaatg ctttgcaatt tcatagaaag aagttgttat
    14581 ttattaatca tttaaaaaaa acagtaacta gaccttattt ctatatatta tcgggcaata
    14641 aattaaaaaa agctttacct tacaataagt aagataattt ttgtgataaa aaaataacga
    14701 aaaatattta aaaatagtgt ctatttcgtg agaaaacaac tatatttttt atgtgttttg
    14761 ctttataatt aaaaaaatat tgtcatttta tggaggaaaa tatatggaaa attatcttag
    14821 ccttttaggc gtcttgattg ttattctcgg gtttgcattt aaattagact cgatattgat
    14881 cgtaatggtt gctttagttg ttacagcttt gactggcggt cttggcattc aaggaatgtt
    14941 agaactttta ggtacaagtt ttgtaaacaa tcgcgggatg gcgattttta tcatcattat
    15001 gttggcaaca ggaacgcttg aaagaaatgg tctgaaagaa tcagcggctt ccttgatcaa
    15061 gcgattcaag aaagtatctg ctggtatgat tgtagatatt tatgggatct ttcgaatgat
    15121 ctttgcggct tttaacgtga gttttggtgg agttgctgga tttgttcgtc caatcatttt
    15181 accgatggca ttggggacca tcgaatcaaa agggttagaa gtccatccta agcatgaaga
    15241 agaactaaaa ggtatggctt cagctatgga aaatgtctgc tggttctttg gccaagtact
    15301 attcatcgga ggagcagggg cgttgctagt ccaatcaaca ctgaaagatc taggatatga
    15361 cgtaacattg ggacagcttg ctttagtgga agttccagtg gctatcacgg ctttagttgt
    15421 ggcaagtgtc tacttctatc ttaaagaaaa gaaattagcg aagaaatatt atccaggaaa
    15481 ggggcaataa gatggacttt tttacaaatc ctgatatttt attaggcgat aaattactgg
    15541 agatccttta tatcgtgatg ggactcgtat tgatttacac tggagtaaga aatttaacag
    15601 ataaaaccaa tcctcatcgt tatgggtcag cctttttctg gacggtctta ggggtagtta
    15661 ttgcaggagg aagatggctt ccggcaatcg tgaacggtgg cttgatcttt gcgatgacca
    15721 taccagcaat tgctaagaaa gtcagcaaag gagaaagccg gcttccatca aaagaatata
    15781 tggaaaaaat gtcagacaaa ctaggaatga aaatttttat tccagctttg agtatcggtg
    15841 tctttgcgat cttgtttgct ctatttacca atttaggtgc tttagtcggc gtgggcgtcg
    15901 gtgtttttgc agccatgcta atcttgatgt tcttttctcg agataatcga ccaagtacat
    15961 ttttagatga tgcagcagat atgctgggca cagtagggcc gttaagcatg ttgccaatgc
    16021 ttttagcttc tctaggtgcg gtatttacaa gcgctggagt tgggacagtg atttccaatg
    16081 cagtaggtac gatcgttcca gaaggaaatg tgaacatcgg catcatcatt tacgctcttg
    16141 gcatggtgat atttacggta atcatgggga atgcatttgc ggccatcacc gtgatgacgg
    16201 ttgggatcgg tgcaccattc gtatttagtc atggagcgaa tccagcgtta ataggaatgg
    16261 ttgcattgac ttgcggtttt tgtgggaccc tgctgacacc gatggctgct aacttcaata
    16321 tcgtgcctgt agcgatgctg gagatgaaag acaaatacgg agtgatcaaa aaccagttgt
    16381 ttattgcgtt atttatgcta gttttccaaa ttgcttatat gattctattt aaataataga
    16441 agagaggggt tgtgacagaa taattgtcgc aacctcttct aacattgtag tggaacacta
    16501 tttttttata agaatatatt aagtttgatc tgttatgtta tttaaactat tgtttccttt
    16561 atttacgctg ttcaaggaaa atggtatgat aaaagataac catatgttaa atcaatcgga
    16621 cggaggaaca acagatgaaa gtactagtaa caggttttga tccatttggt ggggacaaag
    16681 tgaatccagc ctatgaagca gtaaaaaaaa tgccagatga aatcagcggt gctgaaatca
    16741 tcaaagtgga agtaccgacc gtattcgaaa aaagcagtca agtagtcaaa gaagccatcc
    16801 aacagcacca accagatgtc gtgatctgtg tcggacaagc aggtgggcgg tcagcagtct
    16861 cgttcgaacg tgtcgcaatc aatctagcag aagcaagaat ccctgataat gaaggaaatc
    16921 agccattcga tacggcttta gaagaaaacg gtccagccgc ttactttacc tcattgccaa
    16981 tcaaagcaat gacaaaaaat gtacaagatc atggtctacc agcgtacatc tcttatacag
    17041 caggaacatt tgtctgcaat gatatcatgt atcgtttgct tcatttgatc gaaacacaat
    17101 tcccggcaat tcgaggtggg ttcatccacg tgccattctc acccgatcaa gtgatcgatc
    17161 gtccagtggg tacgccttct atgtctttag aagacattgc ttcttctttg acttatgcag
    17221 ttgaagcagc agtagaaaat aaagaagata tttccggaaa tgccggaaca actcactaat
    17281 ttagcaagaa acactggctg gttgctaacg ttatgttagt agccagcttt ttgtaattag
    17341 aaaggaaaga aacagccgtg gaagaatggc aatccgtctt tgaagaatgg tttccaaaag
    17401 aaatcagcaa aagctatccc atcaaaattt ctaaacaata cacaagcagt caacgttggg
    17461 agatctatgc aaaattaacg aaaaaacagc gggaactagt cgataagcac cgccgatatc
    17521 taatcagctc tcgttttatg gaagaacatt atttagctgc aacagattgg gtgttttctg
    17581 acttcaagat caatccgttt tttcgaacaa aacgaagcca gcaaaaattg tattgtgagt
    17641 gtggtagaga attaaaagtg caatacatcg ttaaatcgcc aaaaaccgga aagatcctaa
    17701 aactaggcat caaccacttt gccgatcacc tgcatgtctc tccgacagtg gcagcttcga
    17761 tccatcaggg aatgacaaaa gttgatctgg ccttagacga attattatgg cttaagcaga
    17821 agaatatcga ttttcctgaa ggactatggc aaaaatactg ttttgtcttg taccaaaatc
    17881 gtcggatgaa acagccatat ttaccggata taaaattagc tcagcggctc gcagaatttc
    17941 gccaagtaga aatgccgatt tacatagcgg attatcaagc attggaaaat gaaatcaaga
    18001 aaataagtga acacatcaat gggcagtcaa aaaagcgaca gataaaaaaa gaactatttg
    18061 acgactttgc agaagaatta gtaaaggatg tagaagaatt cttgatcaac tatcgcgcat
    18121 ttttacgtaa agattggcaa tccatcgtat atgaagaagt gccagtacat cctaatgctt
    18181 attttgaaac atttatttcc gtgttgagaa agacaaaacg tcaacgaaca ccagaagtca
    18241 ctgctcagat ggaatatttt gcgaaaaacc aacgattcat ccaaccaaaa atctatctat
    18301 ttatttggaa gcaatattgt cgctatggtt ttacagaagg gttcttcgac agcattcctc
    18361 ggatagtcag aaatggtttt ttaaaagtat tgcgtaaaga acgagaagct atacaatctg
    18421 ctgataagaa agaccgtaca gtctcaaaag aaaaatggca actagtagtg aaagatatcc
    18481 aatctggaaa tgtgcaagag acaatcgaca aatggaaagg aaaacattat cgttttactg
    18541 aagctcagaa acaagcgttg gaatattatc agaagttaga agaatcactg cgtttcaatg
    18601 atgaggcaag aaagtatctg aaggaattac tataaaaaa
//
LOCUS       DAFCLG010000046        17977 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFCLG010000046 DAFCLG010000000
VERSION     DAFCLG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.046"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            97..2424
                     /locus_tag="LND56_001479"
     CDS             97..2424
                     /locus_tag="LND56_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6743409.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2550..2879
                     /locus_tag="LND56_001480"
     CDS             2550..2879
                     /locus_tag="LND56_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK6743410.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2919..3923
                     /locus_tag="LND56_001481"
     CDS             2919..3923
                     /locus_tag="LND56_001481"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6743411.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3920..5349
                     /locus_tag="LND56_001482"
                     /pseudo
     CDS             3920..5349
                     /locus_tag="LND56_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5555..6728
                     /locus_tag="LND56_001483"
                     /pseudo
     CDS             5555..6728
                     /locus_tag="LND56_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(6890..7345)
                     /locus_tag="LND56_001484"
     CDS             complement(6890..7345)
                     /locus_tag="LND56_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743412.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7934..8356
                     /locus_tag="LND56_001485"
     CDS             7934..8356
                     /locus_tag="LND56_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743413.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8522..9715)
                     /locus_tag="LND56_001486"
     CDS             complement(8522..9715)
                     /locus_tag="LND56_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6743414.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9939..10316
                     /locus_tag="LND56_001487"
     CDS             9939..10316
                     /locus_tag="LND56_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6743415.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10304..10642
                     /locus_tag="LND56_001488"
     CDS             10304..10642
                     /locus_tag="LND56_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6743416.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10776..11219
                     /locus_tag="LND56_001489"
     CDS             10776..11219
                     /locus_tag="LND56_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK6743417.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11481..12296
                     /locus_tag="LND56_001490"
     CDS             11481..12296
                     /locus_tag="LND56_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6743418.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12306..12968
                     /locus_tag="LND56_001491"
     CDS             12306..12968
                     /locus_tag="LND56_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6743419.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13173..14837
                     /gene="alsS"
                     /locus_tag="LND56_001492"
     CDS             13173..14837
                     /gene="alsS"
                     /locus_tag="LND56_001492"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBK6743420.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14850..15560
                     /gene="budA"
                     /locus_tag="LND56_001493"
     CDS             14850..15560
                     /gene="budA"
                     /locus_tag="LND56_001493"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBK6743421.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15690..16187
                     /locus_tag="LND56_001494"
     CDS             15690..16187
                     /locus_tag="LND56_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK6743422.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16372..16632
                     /locus_tag="LND56_001495"
     CDS             16372..16632
                     /locus_tag="LND56_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743423.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16994..17233)
                     /locus_tag="LND56_001496"
     CDS             complement(16994..17233)
                     /locus_tag="LND56_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743424.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            17519..17833
                     /locus_tag="LND56_001497"
     CDS             17519..17833
                     /locus_tag="LND56_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743425.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         6094 a         3103 c         3540 g         5240 t
ORIGIN      
        1 aaagccccaa actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt
       61 aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa
      121 aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa
      181 agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc
      241 ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca
      301 gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct
      361 agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa
      421 gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag
      481 acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat
      541 acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat
      601 aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc
      661 cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc
      721 tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa
      781 agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact
      841 ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat
      901 gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg
      961 gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca
     1021 aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc
     1081 actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta
     1141 gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat
     1201 caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt
     1261 gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt
     1321 tcagcgacag aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat
     1381 ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat
     1441 aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct
     1501 gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac
     1561 aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa
     1621 aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca
     1681 aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca
     1741 tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca
     1801 tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt
     1861 tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca
     1921 gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac
     1981 aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact
     2041 tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat
     2101 gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa
     2161 tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg
     2221 attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct
     2281 aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc
     2341 tttgtctttt taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag
     2401 aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg
     2461 ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat
     2521 cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca
     2581 gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg
     2641 ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta
     2701 gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag
     2761 aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca
     2821 aagatgagca aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag
     2881 taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata
     2941 aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat
     3001 taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc
     3061 tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata
     3121 cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca
     3181 agattcatac atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa
     3241 aagatcttag ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag
     3301 aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag
     3361 tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa
     3421 cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc
     3481 aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt
     3541 ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg
     3601 agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa
     3661 aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa
     3721 atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa
     3781 aaaatggagg aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag
     3841 ggttgttgct agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa
     3901 acaataggag ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac
     3961 tgctatcatg ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta
     4021 tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca
     4081 gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc
     4141 gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt
     4201 ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg
     4261 actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag
     4321 cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca
     4381 tcagaccctg acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa
     4441 agcaatatca aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg
     4501 caattccaga tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt
     4561 gtcgctctct atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc
     4621 atcagtgtca atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat
     4681 ggttatcaat taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg
     4741 caacaaacaa ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt
     4801 gtagtcaatc gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac
     4861 tacggtgatt tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca
     4921 gaattttcta ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat
     4981 cttgcctcag gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa
     5041 gaagtgaaat tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg
     5101 gcatactgcc ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct
     5161 gcgtacactg aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat
     5221 aaccaaccta aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg
     5281 gtaggtactg ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca
     5341 gaagcttaaa ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg
     5401 ggagaaaggt tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag
     5461 gaggaactat tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc
     5521 tcttcatata catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact
     5581 ggacatcttc atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt
     5641 agaagattca ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc
     5701 atcgaataag aattcagagg aaataaaaaa aacagcagga aaaaatgaca aagaaaaacc
     5761 aacagttggc agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg
     5821 caaaagcatt aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca
     5881 tcgaaatccc aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt
     5941 tgcaaaaagg aacgtcatta ttggaagaaa gttcttatcc gactggcggg aaaaacaccc
     6001 atgccgttct ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta
     6061 agttgaaaaa aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag
     6121 tagaaaaaat ccaagtggtc ttacctgatg aagtagattc tttgggtatc caaaaaggca
     6181 gagatctcgt gactttgctt acctgtactc cttatatggt caatacacat cgactgcttg
     6241 tgacggggca tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg
     6301 atcaatggaa aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg
     6361 gcttagtttg gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc
     6421 ctttatcttt ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg
     6481 taaaaacgct caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt
     6541 ctgatgaata cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac
     6601 atgaggagct cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga
     6661 aattgaaaaa aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga
     6721 aagaatgaat gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa
     6781 gctgacgaaa tggacgtgta aactgctatt tttggtttac tatctggaac gccagcttct
     6841 atttttttat gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat
     6901 aacatttgga gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat
     6961 acgtaccatt tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag
     7021 tttgttcatt attttgctca ggagctgcgg cttgtgcctg ctgagaggcc tgttcagctg
     7081 ctgcttgctg tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7141 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7201 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7261 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7321 acaaagcaga ttccgcttct gtcacttttt tttctagtgc agctttttcc gctttttgtt
     7381 tctcttcttc ttttttcttt tcaatttgtt cttgttcttt cgatgaatct gccgtacttt
     7441 cggagatcgt ggtactgctg ctggaagcct gagctgaact tgtgctttga gtagtagacg
     7501 tagtagcacc acacccactc aacaaacttc ctgcgaccaa aaacaatgca actaattttt
     7561 tcactaaatt tcctccttaa gtaaaaaaca accgttttca ataaatgtcc ttttcaattg
     7621 tttacttact tgttataata attgtgttat gaatagtgat tgaggaaact taatctaatc
     7681 ccgttttgat acacgggatt ttttgctata gttctgttat taagtatgca ataataaaat
     7741 atgtacgtta ttttattatt agattattga catttatata ataaacgatt aaatgattca
     7801 tatcaataga atacttagga aaaaaagaga aataataaca ggtttatgca ctattgacgg
     7861 tgtgacaata tgatatttgt atccgttttt ttcttgtatg tagtatagta aagtagatac
     7921 aggaggagaa aatatgcaga cttttttaac aaaagtttct caaaagaaaa ttaaaatgat
     7981 tcatttactg cttgaaacag aacgttggtg ttcaatagaa gaactacaaa acgaattgaa
     8041 ggtatcaagt aagtctatct tgaattatct aacagattta gaagaattgt tccaaaaata
     8101 tcctgataag gtcattttga agaatgagaa taatcggcgc ttttctatgg aaaagcagga
     8161 gaacttccca atctacatta tttatcttca tttctacaga aaatcatata attatcattt
     8221 gattgaattc atgtatcaac atcctgagaa aaatttggaa gattatgcag acgctcaata
     8281 tacgagtgta tccacagttt ttcgttatgc taagttatta gtaaagtact ttgaaatgac
     8341 ggacgtcaaa gaatagtagc cgccagatag aaggaaatta gcagttgaaa aagctgccct
     8401 gcatcaagta atacgaccag ccaaacggaa acagtgtctc atgtttttga tttggctggt
     8461 cttattaccg cagatgcaag cttttgccac acctctttct tgtcatttac agactcaact
     8521 atcaatgctg caagctttcc cttaatgctg taacaccagt aagagttgtc aagaccagca
     8581 gactgactag gtaaataccg ctcaaaaaca agattacggc agttaaacta taatgatcca
     8641 tcaagaaacc gatgacgata gaagaaaaac caccaacagc tcgtccaagg ttcataatga
     8701 aattattcgc cgtcgcgcgg atttctgttg gatacaaact actaatcact gcgccatatc
     8761 caccatacat gccattgata aagtagccga ctatcacagc ggctgctacc aaactccact
     8821 ggctatgggc taacacaagt agatagacag ataaggatga acaaattaaa aacaaagaaa
     8881 aggctagttt aggccccaat ttatccatga ttatgccaaa agtcatcatc cccaaagaca
     8941 tgcctaaaat agtactgacc atccacaatg aagacccaga aaccgaaagc cctaatcgat
     9001 tttgcataat tgaaggcagc caattcatta acccaaaata gccggcaatt tggacagtta
     9061 ccatgatact aaggccacat gtttgccaag caagatgttt tgattcaaac agtctgctaa
     9121 gtttggtttt cttttcggca gattgtacag atttgaattc ctttggttca gtcaagccgc
     9181 gacgaatgaa caagaccatc aacaccggca gtacacctac gacatacaac atcttccaac
     9241 caaacgttgg aatgatcaca gcagctaaaa tcgcagccaa agccgcaccg acctgcgcac
     9301 caataccagc aaccgaagaa gctcgtccaa tttgtttttt tgaaaaactt tcggaaatca
     9361 aggacataca cgccccgtat tctcctcccg ctcctacacc tgcgataaag cgaaagagat
     9421 aaaccaagta gatgtttgaa gcaaaataca tcaataaaga agcaatcgaa aacagaagga
     9481 ccgtttgaga aaatactttc acacgtccat atttatcagc tatgatgcca aaaaaaatac
     9541 ctccaatgag catccccaaa ttagtgatcg ttgaaatgaa acccgcttgc gtaccactaa
     9601 tatgaaaaga agtaatcatt gaagataatg aaaaagctaa aaacataata tccatattct
     9661 ctaaagcaat tcccgccgat gtcgataata ataactttcg ctgatacgtt gtcattttta
     9721 tacccctttt cctaaaattt ccctaaattg ttaaaagtat atccaaaaaa gaataaaaaa
     9781 acaagacttt tttatttgaa ataatacaca aacaaccgat atagataatc cagcttagtt
     9841 ttaatagaaa tgagtattga cttggagtac acttaaagtt gtagaataaa taaaatacat
     9901 aagtaaagtg gcaagaaatt aagagaaaga gtgatcagat gaatatcaaa caagtcagtg
     9961 aagaaaaggg catttcggcc gatacattgc gctattatga acgaatcggg ttgattccac
    10021 cagttaatcg gacaaaaggc ggaatccgtg attatacaga agaagaccta aaatgggtcg
    10081 actttacgat ctgtatgcga aatgccggat tatcgatcga gtctttgatt gaatatattc
    10141 gcctatctag cgaaggagaa gcaactgtat ttgagcggcg taagcttttg attgaagaaa
    10201 gtgaactttt gacgaaaaaa attgctgaaa tgcaagaatg tttggaacgt ttacaaggaa
    10261 aaatcaagaa atatgatcgt gtgttatcta gaaataaaat ggaatgtatc aagtaaaaaa
    10321 atcaaggagc atcgctatgg agttacgctt actaaaatat ttttggacaa ttgcagaaga
    10381 aggaacaatc tctcaagccg cagaagttct acatcttaca caaccaacct taagccgaca
    10441 actgaaggag ttagaagagg aactaggaac agaactgttt gtacgcgaaa accgcaaaat
    10501 gatcttgaca gaagcaggtt attttctaaa aaagccgagc agaagaaatt ttagatttaa
    10561 cgaatcaaac cactcaagaa ttcgagaaca gaaaaaaaca attattcagc ggccgattta
    10621 cgatcggctg tgtggaagct gataattcga atacattggc actattacta gaaaagttga
    10681 tccgtgatta tcccagcgtt caatttcata tccacagcgg aacaagtgat gatatcacgg
    10741 agtgactaga aaaaagcctg ttagatttag cgattttgct agatccagta gatacagaga
    10801 aatacgaaac gattttgtta ccaagaaagg aaaaatgggg attgctcatt ttagaaaatt
    10861 ctgttttagc aaaaaaagac gtgataacaa aagaagactt tgcgaatgta cctcttttga
    10921 tctcaaagcg tccagaaata cagcgaatgt ttgctgactg gttaggcata aaaaaggagc
    10981 agttgctgat cacaggaacg tacaatttga ttttcaatgt atttgcgcta attgagaatc
    11041 aggtaggtgc tgctttagtc atagaaggtg ctgtaagtca tctttcaaag cgagtgacat
    11101 tcgttccgct tgacccaatg attgaaacga actgcatgct agtctggaaa aagaatcgga
    11161 ttttatcacc tgtagtcaat gaattgattc accgtttcaa agaagctttt gaacagtaat
    11221 aagtatctct aatcgaagaa atttcaatac aaaagcgaga cgtatgacag tcttgctttt
    11281 gtattttttc atttttaaat caaaaaaaat aaagagattt acataacttg ttaaataagc
    11341 gcttacaaac ataagaagaa cggaaatgaa tcagctataa taaccgttgt tctggattgt
    11401 aatcattaca aagaaatgaa gattataact ttggaggtgt tcgaaatgaa agaaaaaaag
    11461 caaaaattcg ttcaaaaaac atgcccttta ttcaatggtt gggtatgatt ctgctgattg
    11521 tattagcttt attgacgatc attccttttt tatggatggt ttctgcttca ttcaaagcaa
    11581 ataatgaagt gttcacgatt ccgattcaat ggatccctaa aacttggcat ttagagaatt
    11641 atctggttat ttggtctcgt attcctttag tgacattttt taagaataca gcttttttaa
    11701 gcgttgtgat cacgttgatt cagttgttta cttctagttt tgctgcttac gggttttcta
    11761 aactaaattt taaagggcgc gatattttat ttttagcata tatcggcacg attgctgtcc
    11821 cttggcagtc ttatatgatc ccgcagttca ttatgatgag aaaagtgggt ttaacatata
    11881 cgttgtggtc gttggtgcta ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc
    11941 aatactacag tagtatccca gattctttat gtgagagtgc gcggatcgat ggcttatcag
    12001 agtggggaat ctatcgcaag atcattttac ctcttacaaa accggcgatt gccagtttga
    12061 cgatcatcac attcgttaat acatggaacg attatatggg gccattcatt tatttgtctt
    12121 ctacggaaaa taaaacaatc cagttagggt tgaaaatgtt tattgggcta tttgatgcag
    12181 agtacgcatt aatcatggct gcctcagttg tttctgttct tcctgttgct tttatatttt
    12241 tagcgatgca aaaatatttt gtagaaggga tcgcttcatc aggaatgaag gaataggagg
    12301 tgattatgaa agagcagcgt tttgacaaaa atgtttatat ggaattcttt tattggttgt
    12361 atcaatcatt ggcaagtagc ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg
    12421 cgttcctttt gtcgatagat ggaaggaatg tcttttcctt tattttttct ttacttttta
    12481 ttggtccagg ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt
    12541 tttcaccagt acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt
    12601 attggctatt tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac
    12661 gtttttctta tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt
    12721 cctttagcat caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag
    12781 ttctacggat agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt
    12841 tactattgac aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag
    12901 gtccttctat cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg
    12961 atctgtagac tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact
    13021 gtcattcttt ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac
    13081 ttttgaatga aacaggttta gaaactgtat caattatttg tgattatttc taactaataa
    13141 ttaaaaataa agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga
    13201 aatctgtagt agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg
    13261 caaaaatcga tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc
    13321 gtcacgaaca aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag
    13381 gagtagttat cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag
    13441 cgacagcaga aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat
    13501 tgaagttgac ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca
    13561 gtgcagaaat ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg
    13621 ctaagagttc aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac
    13681 ctgtccaagg gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag
    13741 atgatatccg ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag
    13801 gtatgcgagg atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag
    13861 cattgcctgt tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc
    13921 atttctttgg tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa
    13981 gtgatctagt tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg
    14041 cggaaaaaga tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta
    14101 tgcagcctga aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt
    14161 cattagagcc tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcaa
    14221 aattaacgga gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag
    14281 aagttattaa cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag
    14341 gtagtcatta tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat
    14401 tcagtaatgg tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg
    14461 tgcgtccaaa tacacagatc gtatcagttt ccggcgatgg gggattttta ttctctgccc
    14521 aggatttaga aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg
    14581 gacactacaa tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag
    14641 actttggtcc agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag
    14701 ctacaagtgt agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg
    14761 taattattga tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac
    14821 cagatcaatt ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga
    14881 cattaggagc tttgatggcc ggcttgatgg atggaacaga aacgattgct catatattag
    14941 aaaaaggaac tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag
    15001 atggcgtagc ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac
    15061 ttacccccta tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa
    15121 ctgcagatgg agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat
    15181 ttttggcagt gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc
    15241 aagaaaaacc ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa
    15301 gcaatgtcca aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg
    15361 cagcgggatt tcacctgcat tttatcgatg atacacatac atttggcgga catgtaatgg
    15421 attttgaagt ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt
    15481 ttccagttga tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag
    15541 aaatccaaca agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg
    15601 tatctcgttt ttttgaggag aaaacctagc gtttacaaaa aaactgagct ataatcttct
    15661 ccatagatca aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat
    15721 acgaaatttt attattacag caacaaggat tattagaagc agaagataac tggttagcaa
    15781 atttagcaaa ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat
    15841 attatttatt cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca
    15901 cacgtatcaa gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga
    15961 tcgttgatga tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag
    16021 aaatcgtagt gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg
    16081 gagtaacgaa agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt
    16141 tattagcaaa ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga
    16201 aacaaaaaag aagagaaatc aagaataaaa agagccggaa agacagtggg catttctgtt
    16261 tttcggtttt tttatttttt tataaaatca caagtgttat tgattttcat aatcatattt
    16321 taatgttatt atcattttaa ataatgaaaa aacattaagg atggatgaaa tatgaataac
    16381 gaaactatca ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg
    16441 attggagaag taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat
    16501 gacgtcgatc gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac
    16561 gtttatgagt tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact
    16621 ttagtttcat aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag
    16681 gcactttatt tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct
    16741 agagaatctg tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca
    16801 gaactgtcct attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc
    16861 atttcaaaaa atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc
    16921 atttgtaaag gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa
    16981 gaagatgaag aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct
    17041 ttaccctgtt ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt
    17101 tctcatttgt agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt
    17161 tctttgatgc agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca
    17221 gaattctttt cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag
    17281 tattaggcaa aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa
    17341 tacatatatg gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat
    17401 taacgattat gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta
    17461 tgatattatc tagaggtaat ttttttgggt atttaaatag ttgctaggag gatagctttt
    17521 ggatttttac agtaatttta tcttaataat agcaatatta ttacttctaa atatttggtt
    17581 ttttgataag agtagaaatg cagggatagg ttttagaacc aaacgaagta cgtcttctga
    17641 aaaaaagtgg gtatattcac agactatttt ttacggaggg gttatttcaa tcagtttact
    17701 ttcttcaaca ctttattctt ttaatgttat agacgtttcc atgtcaaatt ttatatctat
    17761 tattggaatc cttatttctg caattattac acaactactt cttgtgtttg aagagaagtc
    17821 aaaaaataat taaagcaatt atgcctttta gtacaggaat tattacttct tacaaatgta
    17881 tagcgaatgt gaagcctgct gttttaattg ttctagaaaa acttttgagt gaacaattat
    17941 tttatgttag gatgaattaa tttctctaga gtttata
//
LOCUS       DAFCLG010000047        17770 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFCLG010000047 DAFCLG010000000
VERSION     DAFCLG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.047"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..871
                     /locus_tag="LND56_001498"
     CDS             59..871
                     /locus_tag="LND56_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBK6743426.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            868..1668
                     /locus_tag="LND56_001499"
     CDS             868..1668
                     /locus_tag="LND56_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK6743427.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1829..3097
                     /gene="rseP"
                     /locus_tag="LND56_001500"
     CDS             1829..3097
                     /gene="rseP"
                     /locus_tag="LND56_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBK6743428.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3165..4874
                     /locus_tag="LND56_001501"
     CDS             3165..4874
                     /locus_tag="LND56_001501"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK6743429.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5086..9438
                     /locus_tag="LND56_001502"
     CDS             5086..9438
                     /locus_tag="LND56_001502"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBK6743430.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9582..10055
                     /gene="rimP"
                     /locus_tag="LND56_001503"
     CDS             9582..10055
                     /gene="rimP"
                     /locus_tag="LND56_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK6743431.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10079..11254
                     /gene="nusA"
                     /locus_tag="LND56_001504"
     CDS             10079..11254
                     /gene="nusA"
                     /locus_tag="LND56_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK6743432.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11276..11569
                     /locus_tag="LND56_001505"
     CDS             11276..11569
                     /locus_tag="LND56_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBK6743433.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11566..11877
                     /locus_tag="LND56_001506"
     CDS             11566..11877
                     /locus_tag="LND56_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBK6743434.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11890..14196
                     /gene="infB"
                     /locus_tag="LND56_001507"
     CDS             11890..14196
                     /gene="infB"
                     /locus_tag="LND56_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK6743435.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14223..14570
                     /gene="rbfA"
                     /locus_tag="LND56_001508"
     CDS             14223..14570
                     /gene="rbfA"
                     /locus_tag="LND56_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK6743436.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14678..14899)
                     /locus_tag="LND56_001509"
     CDS             complement(14678..14899)
                     /locus_tag="LND56_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6743437.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15347..17710
                     /locus_tag="LND56_001510"
     CDS             15347..17710
                     /locus_tag="LND56_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBK6743438.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6059 a         3150 c         3914 g         4647 t
ORIGIN      
        1 tagatattgc tataatactt aagatagtgg gaatctttta agtataggag gaaaaagaat
       61 gttacgtttt tttccgcaaa aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa
      121 taccgatgga ccaattcctc agcacattgc catcatcatg gacggcaatg gtcgttgggc
      181 gcaaaaccgt cgtttgccac gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa
      241 agtcacaaaa aaagcatcaa ggcttggagt gaaagtgttg acgctttatg ccttttcgac
      301 tgaaaactgg aagcgaccaa aagatgaagt aagttttctc atgcaactac ctgtcgactt
      361 ctttgatacg ttcgtaccag aattgattaa agaaaatgtc aaagttcatg tcatgggtta
      421 tgaaaacgta ttgccagaac atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa
      481 aaacaatact ggaatggtat tgaattttgc tttgaattat ggaagtagag cagagatcgt
      541 gactgctgtc caagaaattg ccgaagaagt agcaaaagga gaaatacacg cagaagaaat
      601 cgatgatgaa ttgattgcta agcatttaat gacaggtttc ttgcctaaag agttacaaga
      661 tcctgagctg atgatccgta caagtggaga agagcgaatt agtaattttc tgttatggca
      721 gatcgcatat agtgagctgt attttacaaa agcgctttgg cctgattttg atggtgcgca
      781 tcttgaagaa gcaatcgcct cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa
      841 aacagaagat gagaaagggg atcagtcatg aaacaacgtg tgatcacagc agctgtggcg
      901 ctagtcttgt ttattccgat tatatggatc ggaggcatag cggttgagct tacagcagca
      961 atcttagcgg tagtaggagt ttacgagctg ttccggatga aaggattgac actcttaagt
     1021 tttgaaggag ttttatccgc cgttggtgca gtgttacttg ttttgccaaa agaaagatgg
     1081 tttttctttc ttccagagaa tgtaagtaca tttatgttat tttatcttat agttatgatt
     1141 cttttaggaa catcagttat ttctaaaaat acgtatacga ttgatgaagc aggttttcct
     1201 gtgcttgtca gcttgtatgt gggaataggt ttccagaatt ttgtcaatgc acgaacagaa
     1261 agcttggctg ttttgatatt tggtctattt attgtttggg caactgatat cggagcatat
     1321 atggttggtc gacgttatgg tcagcataag ttaagtcctg atatttcgcc caacaaaacg
     1381 attgaaggtg cgctcggtgg tatcggtagt gctgttgtag tcgcatttct ttatttattg
     1441 ctatacccag caaaagaact attcaatcat ggtatgtttg tcatgcttct gatgacgatt
     1501 attttttcag cagtcggaca atttggtgat ttggtcgaat ctgcaatcaa acgtcattat
     1561 gatgtgaaag actcgggaaa tatcttacca ggccatggtg gaatcttaga ccgtttcgac
     1621 agtttgctgt ttgtatttcc tatcatgcat ttatttggca ttatttagta agtatacctg
     1681 cgtttctttg tgaatttcat aaaagacgca ggtttttatt tttcattata ttgatactat
     1741 tggtgtcttt ggtatactat ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca
     1801 caaaaaacaa gtaaaggaga aaaaagctat gaaaacgatt ctgacattta tcatcgtttt
     1861 tggtatatta gtgattgttc atgagtttgg tcatttcttc tttgcgaaac gatcaggaat
     1921 cctcgtccgc gaatttgcta tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga
     1981 cgggacaaca tatacgcttc gcctattacc tattggcggt tatgttcgaa tggcaggcaa
     2041 tggggatgat gaaacagaaa tggcaccagg aatgcctctt tctcttttac taaattctga
     2101 cggcatcgta gagaagataa atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat
     2161 ggaattgagc cgttatgatt tagaagatga gctgactatc actggttatg tgaatggaga
     2221 cgaaacagaa gtagtaacct accctgttga tcacgatgcg acgattattg aaaacgacgg
     2281 aacagaaatc agaattgcgc caaaagacgt acagttccaa tcggctaaat tgtggcagcg
     2341 tatgctgacc aactttgctg gacccatgaa taactttatt ttggcaatcg tgttattcat
     2401 cattctggca tttatgcaag gtggtgttca agtgacgaac actaatcgcg taggagagat
     2461 catgcctaat ggagctgcgg cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt
     2521 ggacggaaaa gaaatccatt catggaatga tctgacaaca gtcattacga aaaatccagg
     2581 taaaacatta gactttaaga tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc
     2641 gaaatctgta gagtcaaatg gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa
     2701 tacaggtttt atggataaga tcattggagg gacgcgtcaa gctttcagcg gttcattgga
     2761 aattttcaaa gccttaggat cactatttac aggtttcagt ttagataaat taggcggacc
     2821 tgtcatgatg taccaattgt cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag
     2881 tttgatggcg cttctttcaa tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt
     2941 agatggcggg aaattagtct tgaatatttt tgaagggatt cgcgggaaac cattaagtca
     3001 agaaaaagaa ggaatattga cattagctgg ctttggattt ttaatgttgt tgatggtgct
     3061 agttacatgg aacgatattc aacgattctt tttctagaaa aagttgacaa atagggtata
     3121 ataacaaacg ataactattt tgaggaaata aaggagtata tagaatgaga caatcaaaaa
     3181 tgttgattcc aacattacgc gaagttccaa atgatgcaga agtattaagc catcaaatct
     3241 tgctaagagc tggatatatc cgccaagtat cagcgggtat ttattcatat ctgccattag
     3301 caaatcgtgt attggaaaaa ctgaaacgca ttatgcggga agaatttgaa aaaatcggag
     3361 cggtagaaat gctgatgcct gccatcttgc cagcagaact ttgggaagaa tccgggcgtt
     3421 acgaaacata tggaccaaac ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc
     3481 ttggaccaac tcacgaagaa acttttactg atttgatgcg taatgaaatc aattcatata
     3541 agaaattacc tttaaatctt tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc
     3601 gcttcggttt gcttcgcgga agagagttta tcatgaaaga tgcatattct ttccatgcaa
     3661 cggaagaaag tttggacgag acatataaag attacgaaaa agcctatacc gaagtattca
     3721 agcgttgtgg cttagaattt cgctcgatca tcggagacgg cggcgcaatg ggcgggaaag
     3781 actcaaagga attcatggcg atctcagaaa tcggggagga tacgatctgt tattcgactg
     3841 aaagtgatta tgctgcaaac ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc
     3901 atgaaacaca gcttgaattg gagaaagtag ctacacctaa cgtaaaatca attgacgaag
     3961 tagcagattt cttttctgtt gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg
     4021 atgaacaacc tgtccttgtt ttggtacgtg gagaccatga agtcaatgac gtaaaactga
     4081 aaaacttttt atctgctgac tttttagaag aagcaacaga agaagaagca caaaaatatc
     4141 taggtgctga gtttggttct gtcggaccag tcggtgtttc agatgacgta aaagtatatg
     4201 cagaccgtta tgtccaagat ttagcgaatg ccattactgg agcgaacgaa acaggattcc
     4261 actataaaaa tgtgaatcca aaacgcgatt tcactgtgct ttcttatgaa gatcttcgtt
     4321 ttgtacaaga aggagatcct tcaccagaca acaacggtgt tcttgccttt actcgaggaa
     4381 tcgaaatcgg acatatattc aaaatcggca ctcgttacag tgaatctatg ggagcaactg
     4441 tactagacga aaatggacga gaaaaatttg tgattatggg ctgttatggt atcggtgtaa
     4501 gtcgtttatt atcagcaatc gtagaacaac attcagatga acaaggaatc aattggccaa
     4561 aaggaattgc gccatttgat ctgcatatcg tccaaatgaa tctaaaagac gaataccaaa
     4621 caagcctgac gaatgaacta gaagaagcga tgacaaaagc tggctatgaa gtactggttg
     4681 atgacagaaa tgaacgcgca ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa
     4741 tcagaatcac tgtcggtaaa aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa
     4801 ctggcgaaat ggttgaagta cgtaaagaag aattggcgaa tactttacca atcctcttga
     4861 accaaggcga ataataagcg ttaaaacaca gaaaataaaa atagctgaat cagctgtttt
     4921 tgacaatctg atgattcgct tttaaaattg gaactgcgat ttgaatcgca gttccaattt
     4981 tttaatgtaa aactcagaaa aaacgagtat actaaaaggt actgaaaaag aagaggataa
     5041 gatgcgatca attgcattgt ttctcatcag aaagggcgta tatctttgtc agaaaaacga
     5101 gatttatttg aaaaactgtt ggaacaaatc caactagagg aagcagaaaa aaatcatcca
     5161 ctgcttactg ctggtgaaat ggatagtgta gtggtccatc gtaaatcgcg tttatgggaa
     5221 tttacgctac atttttccaa aattttgcct attatgctat atcggtcatt gactcagcat
     5281 ctgactatcg cttttaaaga tattgcacaa gtcaaactga atatcttagc agatgaccag
     5341 acattcgatg aacagctctt gcaagattac tgggcccaag ctttggaaaa tcagcaatgc
     5401 gatacaccac tagcacagca agtattgaaa acgcaggtac ctgtgataaa agatcgtaaa
     5461 gtcatcttgc caatcgacag tacaggagct atttcttatt tgaagcaaca atacttgcca
     5521 ttagtagaag agttgttcgt cagttacgga ttcccaaaat ttcgtatcga accagaagtg
     5581 gatgagcagc aagcggaacg tgtattgaaa ctttttgaag aacgcaaaca agaacaggca
     5641 gaagccttta tgaagcaagc agctgaaagt ctagttgtcc acgaacaaaa gaaaaaggaa
     5701 agaaaagagc aatcgccagc tcttgaagga cccatccagc taggacgaaa tattccagcc
     5761 gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt cacacttgag
     5821 gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat cttgactttg
     5881 aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg cgaaaaagat
     5941 gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg aagtattcaa
     6001 gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga agtcaaacat
     6061 acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca tgttcatagc
     6121 aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca agcaggaaaa
     6181 tggggacacc gagctatcgc cattacagac catggtggtg cgcaagcctt tccggaagca
     6241 catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc gaatgtcgta
     6301 gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga agccacatac
     6361 gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat catcgagcta
     6421 gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt tatcgatcca
     6481 ggacatccat tgtcacgaac aacggtagat ctgactggta tcacggatga aatggttcga
     6541 gggtcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa agatacaatt
     6601 ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta cgcaaggtat
     6661 ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag atatctgtat
     6721 cctcaattca aacgtttcgg attaggtgtg ttatctaaaa agtttggtgt tagcttggaa
     6781 cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg gatctttgtg
     6841 aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga gcatatcgga
     6901 gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc aaaaaatcag
     6961 gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata cttcgaacgc
     7021 gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt gatcggctca
     7081 gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga agaggcaaga
     7141 aaccgagcaa aattctatga ttatattgaa gttatgccaa aagcggtcta tgcgcctttg
     7201 atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca aaatttggta
     7261 gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca ttatctgaac
     7321 gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc gaatccgttg
     7381 aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct aactgcattt
     7441 cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa taaaatcgca
     7501 gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa gattccaggt
     7561 tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta cggagatccg
     7621 ttacctgaga tcattcaaaa acgattagaa aaagagttga attctattaa tggtaatggc
     7681 ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga agatggctat
     7741 ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac aggaatcact
     7801 gaagttaatc cattagcccc tcattattat tgtccagagt gccaatattc cgagtttttt
     7861 gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc aaaatgtggt
     7921 gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg attccatgga
     7981 gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga agcccataac
     8041 tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat tggtacagtc
     8101 gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa tctgcagttt
     8161 cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg gacaaccggc
     8221 cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga ttttacgccg
     8281 atccaatatc cagcagacga tcaaaattca gaatggaaaa cgactcattt tgatttccac
     8341 tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc aactgttatt
     8401 cggatgcttc aagatttatc agggatcgac ccacaaacga tccctacgga tgacccggaa
     8461 gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca aatttattcc
     8521 aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg gatgttagaa
     8581 gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc tcatggtaca
     8641 gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac actggcggaa
     8701 gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt agatagcggg
     8761 atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat ccctgatgaa
     8821 ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg gtatatcgat
     8881 tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta cgtattgatg
     8941 gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg tgcatacttt
     9001 tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga tgctgtgaaa
     9061 caagcaatga aagaaatcac ggacaaggga ttagatgctt ccgtaaaaga aaagaatcaa
     9121 ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt tggaatgatt
     9181 gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt gatcgcacca
     9241 tttagagctg tcccaagttt gggaaccaac gtggcgaaac aaatcgttga agcaagaaaa
     9301 gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc aaaaacattg
     9361 atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa tcaattatcg
     9421 ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa gcagagtatg
     9481 gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg ctcactcttt
     9541 ttaatggaat tacgcactta atttttaagg aggcgaaact tttgagtagc gtcgttgaaa
     9601 cagtcacaga aatggtgaca ccgattttag atgaacaaaa ttttgaactt gtcgaagtcg
     9661 aatttgtaaa agaaggaaaa agctggttct tacgagtatt tattgacaaa gaaggcggta
     9721 tcgatatcga agaatgcgcc tttgtcagtg agaaactgag tgaaaaactt gatgcagcag
     9781 atccagatcc gattcctcaa gcttatttcc tggaggtatc ctccccggga gcagagcggc
     9841 cattgaaaaa agaaagcgac tatgaaaagg cacttggtga ttatgtccat gtctccttat
     9901 accaaccagt agatggcgaa aaacaatatg aaggatttct tcaatcattt gatgctgaac
     9961 agcttacatt gaaaatacgc ataaaaacac gagaaaaaga attgactttt gaccgaaaaa
    10021 atattgcaaa agctcgctta gctatccaat tttaatcacg gagaggaatc agaaaaaaat
    10081 gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga tctcaaaaga
    10141 aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc actatggcca
    10201 agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg tctatgcggt
    10261 aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga aagatgcact
    10321 tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa caccaaaaga
    10381 ttttggacgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg tacgtgaagc
    10441 agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca tgcaaggaat
    10501 cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg aggcagttct
    10561 ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa tcaaagttta
    10621 tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc gcagccatcc
    10681 agacctattg cgtcgcttat ttgaacaaga agtaccagaa gtctatgatg gaatcgttga
    10741 aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc gctcaaatga
    10801 tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag ttcaagcaat
    10861 cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag atccagctgt
    10921 attcatcgca aatgcattga atcctgcaca agtcatggat gtgatcttcg atgagacaaa
    10981 tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa tcggtaaacg
    11041 cggacaaaat gcacgtttag ctgctaaatt gactaaccat aaaatcgata tcaaatcaga
    11101 atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag aagaactgca
    11161 tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa ttgcattcaa
    11221 caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca aagagatgaa
    11281 acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac ctaaaaaaga
    11341 gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa ctggaaaaat
    11401 gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag cttgggataa
    11461 aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc aagaacttct
    11521 tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa tacagttaat
    11581 cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact aatcacgggt
    11641 gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt tgtggcaacc
    11701 gatgcaagtg agaatacaag aaaaaagata aaagataaga gttcatatta tgaagttcct
    11761 tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc tcgcaaggta
    11821 ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga aggttaggaa
    11881 ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa aaaatctagt
    11941 aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca tatgggtgcg
    12001 attactagca gtgatgagaa gaaattgcaa caagcgttca aaaacccaag cacgaaacaa
    12061 caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga tcaacgtaaa
    12121 aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca agatcgcggt
    12181 caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa taatacggga
    12241 aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa ccgtactaac
    12301 aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa ccgtaatcgt
    12361 tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc tgtaccacca
    12421 cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa cgtagcagat
    12481 attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt tatgcttggt
    12541 gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt agcgacggac
    12601 tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga taaattcttc
    12661 gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtagt aacgattatg
    12721 ggacacgtag accacgggaa aacaacctta ttggatacat tgcgccattc acgtgtaaca
    12781 agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga tattgatggt
    12841 aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat gcgtgcacgt
    12901 ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg cgtcatgcca
    12961 caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat cgtcgctgta
    13021 aataaaattg ataaaccagg tgcaaatcct cagcatgtta tgcaagaatt aagtgagtat
    13081 gaattaattc ctgaagcatg gggtggagaa acgatttttg ttgagatctc agcgaaattc
    13141 ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt agaagattta
    13201 aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct tgataaaggt
    13261 aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcacgttgg tgacccaatc
    13321 gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg ccgtcgcgat
    13381 aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt acctcaagca
    13441 ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg tgaagaacgc
    13501 gcaaaacgtg cattattgga aaaccgtgct gccacaagcc gtgtgacttt ggacaactta
    13561 tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa agcagatgta
    13621 caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga aggtgtccgt
    13681 gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac cttagcagca
    13741 gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc aaaagtgcaa
    13801 gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc catcgaagaa
    13861 atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat caccggtcaa
    13921 atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg tgcctatgta
    13981 acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg aatcgtaatc
    14041 tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga agtcaaaatg
    14101 ggttatgaat gtggtgcgat gattgaaaaa ttcaatgaca tcaaagtaga tgacgtaatt
    14161 gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg aggccaatca
    14221 ttatggctaa ctaccgtgat cgccgagtcg gtcaagaaat cttaaaagaa gtcaatgata
    14281 ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca gatgtgaaag
    14341 ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac ttagcatcag
    14401 ataagaaaaa agcacaagat ggattgaata aagcatcagg attgattcgt cgagagttag
    14461 ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat gaatcagttg
    14521 tctatgggaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa tcaaaaaatc
    14581 ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa gacgtattac
    14641 ctaagagttc catgacttta gtctggtagc gtgtcagcta accaaaaaag agaaaaagaa
    14701 aagtaaaaaa ttcgactttc ttttttctat agtatataaa tttttgcata tagataaaga
    14761 tcaagatacc gataacggaa gctagattgt aatctatttt agtgacagtc aggtatagcc
    14821 aagtaactag aagatcagtg gaacctgcac cataaaagtt tgagtttgtc ggtccgttga
    14881 cagttaaaag aaaaatcacg ttgaagttat tgatatttcc gattacgccg cttcgatttg
    14941 gtcacgatcc aaattttgga taatccctgt agaaattagc atagtagctg ggataccaat
    15001 tcacatattg acaaagataa cagagacttt tacataaaac ggatcaatca ggaaaggaat
    15061 tgctcgatcc agccccagtt ttccaacaat gtgttaactg gcctagcgtc attcagcaaa
    15121 ttatgcatga tgaggagcga aatgaactgc ggaacagcca ttgtgataac gacaagtttt
    15181 ttggatcaat gtaaaaaacg tcagcgaaag gaaaaatttc gtggtttttt atacctgttc
    15241 atttggctag atataaaatc caaacaaatg aacagaagtg cttattaggt ttcaattggg
    15301 agtatctgcg ctagaataag agtaataatg aaaaggagtg ttatgtatgg attattctag
    15361 taaagaatac tttgataaaa tgacggcttg gtggcgtgct gctaattatc tttctgttgg
    15421 gcaactttat cttaaagata accccttact gagacgtaca ctgaaaccag aagatgtcaa
    15481 aaaacatcca atcggtcatt ggggaacgat tcctggccaa aattttattt atgtccattt
    15541 gaaccgagtc atcaataaat atgatttgaa catgttttat atcgaagggc caggacacgg
    15601 cggacaagtc atggtatcga atgcttattt agatggaagt tacacagaga tctatccaga
    15661 agtaacggaa gatgagacag gaatgcaaaa attattcaag cgattttcat ttcctggagg
    15721 cattgcttct catgcagcac cagaaacacc aggttctatc catgaaggcg gagagcttgg
    15781 atactctctt tcacatgcag tcggagctgt attagacaat cctgaagtaa tctctgctgt
    15841 cgtgattggt gatggtgaag cagaaaccgg tcctttagct ggaagctggt tctcaaatgt
    15901 ctttatcaat ccagtgatag atggagctgt tttaccgatc cttcatttaa atggtgcaaa
    15961 aatagccaac ccaacgatct tggcacgtaa atcagatgga gaattagcaa attactttaa
    16021 cggacttggc tgggagcctt tctttattga aggaaatgat ccagaaaaat taaatcctgt
    16081 tatggctgaa aaaatggatc aagcaatcga gaaaatcaaa tccatccaaa aagaagctcg
    16141 cctgaagaca gcaacagatg tcgtgatgcc aaaatggcca gtattgatcg tacgcacacc
    16201 aaaaggatgg accggtccaa aagaatggga cggggaacca attgaaggaa ccttccgagc
    16261 acaccaagta ccgattcctg tagatcaaga acatatggat catgcagatg ctttactccg
    16321 ttggctgaaa tcctatgaac cagaaaaatt atttgatgca caaggacgta ttttagaaga
    16381 aatcagagaa attgcgccta ctggtgacca aagaatggca aaaaatccta tcaccaatgg
    16441 tggaatcgat ccaagaccgc ttattatgcc agattggaaa aaatatactt tgcaatttga
    16501 aaaaccaggc tccgttaaag cagaagatat gaccgaactt ggtaaattcg ttcgcgagat
    16561 cattgagaaa aatccagaaa atttccgtat atttggacca gatgaaacaa aatcaaaccg
    16621 attgaatcaa gttttcaaaa cgacaaatcg ccaatggatg gaaaaaatcg aaccagaaaa
    16681 tgatgaatgg ctttcgccat ctggtcgcgt catcgacagt caattatcgg aacaccaaga
    16741 tgaagggttc ttagaagggt acgttctgac tggacggcat ggtttttttg caagttacga
    16801 atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga tacggaaatc
    16861 acatgacctt tcttggcgaa aagattatcc ttcgctgaat ctcattgcaa gctcaacagt
    16921 cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga cacatttggc
    16981 agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata cattgctcgc
    17041 agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct cttccaaaca
    17101 tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg aaggcttgaa
    17161 aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca ttgctgctgc
    17221 aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg aagaattccc
    17281 aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc atccaagtca
    17341 agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag acaaaccgat
    17401 cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg atcgtcataa
    17461 ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac catttgacat
    17521 gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt taaaaatcaa
    17581 aggaagtcaa gcagaagatt ttgcggaaaa aatggatcag aaattacaag aacatcaaaa
    17641 ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat ggaaaaatct
    17701 tgaccaataa aatgataaaa tcgatcccgc aaaacagaat tttttacatt gtattatttg
    17761 tcaaattaag
//
LOCUS       DAFCLG010000048        17727 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFCLG010000048 DAFCLG010000000
VERSION     DAFCLG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.048"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..122)
                     /locus_tag="LND56_001511"
     CDS             complement(<1..122)
                     /locus_tag="LND56_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6743439.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKE"
     gene            complement(227..1090)
                     /locus_tag="LND56_001512"
     CDS             complement(227..1090)
                     /locus_tag="LND56_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6743440.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1286..3385)
                     /locus_tag="LND56_001513"
     CDS             complement(1286..3385)
                     /locus_tag="LND56_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6743441.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3605..4714)
                     /gene="rpoD"
                     /locus_tag="LND56_001514"
     CDS             complement(3605..4714)
                     /gene="rpoD"
                     /locus_tag="LND56_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK6743442.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4738..6579)
                     /locus_tag="LND56_001515"
     CDS             complement(4738..6579)
                     /locus_tag="LND56_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK6743443.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6761..9076)
                     /locus_tag="LND56_001516"
     CDS             complement(6761..9076)
                     /locus_tag="LND56_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6743444.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9261..10121)
                     /locus_tag="LND56_001517"
     CDS             complement(9261..10121)
                     /locus_tag="LND56_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBK6743445.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10180..10812)
                     /locus_tag="LND56_001518"
     CDS             complement(10180..10812)
                     /locus_tag="LND56_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBK6743446.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            10979..11740
                     /locus_tag="LND56_001519"
     CDS             10979..11740
                     /locus_tag="LND56_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6743447.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11766..14357
                     /gene="mprF"
                     /locus_tag="LND56_001520"
     CDS             11766..14357
                     /gene="mprF"
                     /locus_tag="LND56_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6743448.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14545..15732)
                     /gene="tuf"
                     /locus_tag="LND56_001521"
     CDS             complement(14545..15732)
                     /gene="tuf"
                     /locus_tag="LND56_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6743449.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16100..16456
                     /locus_tag="LND56_001522"
     CDS             16100..16456
                     /locus_tag="LND56_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6743450.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(16697..17701)
                     /locus_tag="LND56_001523"
     CDS             complement(16697..17701)
                     /locus_tag="LND56_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBK6743451.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4841 a         3682 c         3087 g         6117 t
ORIGIN      
        1 tgctctttct cattttccaa aagcacaagc agagtagctt ctctttgcgg tgtcagctta
       61 aaccccgctt catgtagctg ctgttttgtt tttttcatcg ccgaaatagc attcatgacc
      121 atttactcaa ccccaacaat ttataataat tacaaataaa aaaagatata acactatgtt
      181 aattaaaatt attataagct agttaagaaa aacatgcaag ggattctcaa ttagattttt
      241 tgactagttt tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa agattaccta
      301 atgcgcgctt aaatgctcct ttgctgatac caaaagcttt catgatttct tctggatccg
      361 atttatctgt aaagttgatt tgctgatctg cagaacgttc taaaatggtt aaaatcattt
      421 gtgcatcatc tgatatagct tcatatgctc ttggcttcaa tgaaagattc agtacaccgt
      481 ctggtcggac accaatcact cgacctttta tctgctcgcc tagacgaggt tcattatatc
      541 gttcagaagg atggataaac ccaagataga aatcttccgt taaaacgtac gtacctgtta
      601 atttcaagcg gtatgctgtt cctatcacat ctttgttttt catttcttct gttccatatt
      661 tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat
      721 caactctaag ggaaatcatc aagcgatccc cttttttagg ccatagctct gtcattgtcg
      781 gcaattcatc aagagatacg acaaagtctt tatctggaag accgatatcg acaaacgcac
      841 ctaaatcttt tcgtgtagca gtaacgattc caaaagcgta atgaccgata cggcttttag
      901 gaatttgtgt agtgatccga ttttcatgtt tttgattttg gtaaccgaat ccttcaactg
      961 cttcaccgat tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt
     1021 ggacaaagta gtttttttca ttttcatcaa taataatacc tgtaaatata gttgctaata
     1081 aatcgttcat ttttttctcc ctctatagtg aaaggttttc ttaatacatt tctttgctat
     1141 cataacataa tgttatctac ttgaatagct gagccttggt ttggaaaggt gaaaaaagga
     1201 gtatgacaac agttgtgtca tactcccctt tttcgaataa acggtgttaa acggcaggct
     1261 cacagcctct ttcatttgcc taaaattaat tgctcccatt tcctccgaac catccagttt
     1321 tacagttgat ggttacgatg taaaatggca aagcactttc tccaatgcct ttcatcaatg
     1381 cagattttgt tgaagtggca tatagattat aatagccttc cccattttcg attctggctt
     1441 tttctaatct gtaacctcct tcaacgatat atccacgttg gatacatgta gcaatcactt
     1501 tgctttggac acctggtgcc cacgtccatg ctggattaga tgcatcggca atatcacttg
     1561 ctctggcaga tttgattggc tgattcgctg agtttgaaga ggaaggggtc gaggatgccg
     1621 cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct gcagctgctg
     1681 cttgagcggc ttgttttgct tgttcttcag ctgctttttt cgcttcttct gcttttttct
     1741 tttcctcttc ttcttttttc tctttatcag tgagcatctt atctacttgt ttcaattgtt
     1801 cattcaactg agcagataga tctgtatttt tcactttttc tactgcttct tttgccgtct
     1861 tgtattggtc gcgtgttgct gaatccacta cttttccgtc tttaaagact atagctgttt
     1921 tttctttagc tgtttgcaat tcagcatatt gatttttagc ttctgtttgt gcttgttgaa
     1981 tcaattttcc aaaatctgtg ttatccgttt cgattgcttg gatctcttta tctgctttca
     2041 acttgatgtc atctgctaga tgatcatcga cgatggcagg tgctatgaac cactcattca
     2101 ctttttggat atctcggatc ttagattgga tatcttggta agttgtttct aactctgaat
     2161 atcctttttc attcttaaga ctggataaat cattttttag ttttgataaa tcttgatgaa
     2221 tcatatctgc acgaataaat tgatggtgat catctgtata aaaggcagca agtttctcat
     2281 gaagttcttc catttgttgc tgtctttttg cttctgctgc tacttcggct tctacttttt
     2341 gatgatggtc ataataagcc cagcttcctc caccgagtag aagtactgct gctaaaacga
     2401 gataaggtac tttttttgat tttttctttt gcgctttatc tggatctgtt ttttctaagt
     2461 ttgattcttg atgtgttttt tcagtatctg gcacgattgt tattgatttc gcttctgctt
     2521 caaacagctt aggctggttc tcttcttttt ctatttttgc ttcaagatca gtaccctctt
     2581 tgcttacaga ttgattttca gcatcggatt cactatttat ttcatcgggc tctgttgact
     2641 ttttgtctgt gttttcgagt atttcttttt tctctgcttc cggcagttta ttcgtttttt
     2701 ccttttctaa ttgtttttca gtaaatactt cagttttttc tgtgatatcc gtttttacgt
     2761 cttttatttt tactgtttct ttacctgtgg cgttttctct atcagagtct ttttcagttt
     2821 cttgttcctt ttctggctct actttgtttt cctgtgcaat tttttcactg atcattttga
     2881 gcagctcttt cgcgtgctct tcttttgtat cttcacgatg tgctttgata taagcggata
     2941 gtatcgaatt ttcttcctca ttatcgacga tttctttcag ttcagctact cttctttttt
     3001 gttttgattc aagcgtttct tcttgattct ttcctgattc ttccgaagtc tcatgaacag
     3061 ttgctttctt ctgttcattt ttctctatat tagttacttg atcgtgagca ggtggttctt
     3121 cgtcttctgg ttctgctttt tcgaataatt ccattacaga ttctagaggt aaatctttca
     3181 attccgacca ttcgattgga tcgtttaatt ctgtatcagg aaagactgca tcttcagttg
     3241 atttatcctg catagctgca tctgtatttg tttcttcaat tgattctttt atttgctgat
     3301 cttctgcagt tatttgatga ccgcagtctg gacagatagt attctgctcc atttttttcg
     3361 atccgcaatt tggacatttt ttcatcactt agcgatcggc tcctttacta ggtttacgct
     3421 accatttaat ctatcatatt ctatacgtca cgccaatagc ttatttttag gatttactta
     3481 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
     3541 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
     3601 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
     3661 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaacact ttccctactt
     3721 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
     3781 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
     3841 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
     3901 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
     3961 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
     4021 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
     4081 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
     4141 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
     4201 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
     4261 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
     4321 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
     4381 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
     4441 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     4501 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     4561 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     4621 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     4681 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     4741 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     4801 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     4861 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     4921 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     4981 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     5041 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     5101 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     5161 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     5221 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     5281 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     5341 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     5401 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     5461 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     5521 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     5581 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     5641 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     5701 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     5761 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     5821 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     5881 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     5941 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     6001 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     6061 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     6121 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     6181 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     6241 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     6301 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     6361 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     6421 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     6481 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     6541 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     6601 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     6661 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     6721 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     6781 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     6841 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     6901 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     6961 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     7021 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     7081 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     7141 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     7201 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     7261 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     7321 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     7381 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     7441 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     7501 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     7561 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     7621 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     7681 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     7741 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     7801 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     7861 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     7921 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     7981 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     8041 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     8101 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     8161 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     8221 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     8281 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     8341 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     8401 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     8461 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     8521 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     8581 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     8641 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     8701 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     8761 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     8821 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     8881 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     8941 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     9001 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     9061 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     9121 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     9181 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     9241 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     9301 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     9361 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     9421 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     9481 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     9541 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     9601 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     9661 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     9721 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     9781 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     9841 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     9901 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     9961 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
    10021 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
    10081 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
    10141 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
    10201 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
    10261 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
    10321 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
    10381 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
    10441 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
    10501 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
    10561 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
    10621 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
    10681 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ctaaaaacaa
    10741 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
    10801 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
    10861 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
    10921 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
    10981 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
    11041 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
    11101 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
    11161 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
    11221 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
    11281 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
    11341 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
    11401 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
    11461 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
    11521 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
    11581 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
    11641 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
    11701 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
    11761 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
    11821 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
    11881 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
    11941 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
    12001 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
    12061 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
    12121 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
    12181 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
    12241 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
    12301 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
    12361 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
    12421 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
    12481 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
    12541 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
    12601 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
    12661 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
    12721 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
    12781 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
    12841 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
    12901 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
    12961 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
    13021 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
    13081 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
    13141 tcgttattgg ggtatacaat ctgccagatt tccctcatcg gcgtcaccat tttatttcct
    13201 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
    13261 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
    13321 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
    13381 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
    13441 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    13501 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    13561 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    13621 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    13681 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    13741 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    13801 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    13861 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    13921 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    13981 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    14041 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    14101 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    14161 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    14221 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    14281 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    14341 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    14401 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    14461 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    14521 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    14581 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    14641 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    14701 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    14761 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    14821 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    14881 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    14941 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    15001 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    15061 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    15121 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    15181 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    15241 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    15301 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    15361 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    15421 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    15481 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    15541 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    15601 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    15661 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    15721 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    15781 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    15841 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    15901 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    15961 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    16021 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    16081 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    16141 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    16201 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    16261 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    16321 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    16381 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    16441 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag gctgggacag
    16501 aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    16561 tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttttt gttatattcg
    16621 tcttttccga tccttctctt gacacaattc atttaactga tcactggtac ttgaacacct
    16681 aatattctag tcatttttat aaacgattca atacttcatc cacatttttg atcatcacgg
    16741 atatcgtacc atccggatta ttcacaaatt caatcaaatc tgggttgcga tatacatcta
    16801 ctggtacgat cagttcgatc ccgtttgata gcttgaattt ttgttttcca aattttttct
    16861 cagaaatttc ttgtacttct tttacaggag gtgcttctgg gacaaatcct tgctcttgta
    16921 cttcttcttt aaaagccatt tttgccgtga tgttttcctt gaagactttt tccgcaatca
    16981 tctcatgatc caaccgacct ttttcttcta tcgtatcata gacagcttct ttcaaatcag
    17041 ccatgatatc aaattcttct gtctcaaatt tttttccaag atgcttggct actttttgga
    17101 tcacttttac attttcttct aaagaaggtg caggaacact ttcgatcact cttccagaaa
    17161 aataaagctc ttttttgcca gaaaactcat attttttttc aattaattcg taggtccaat
    17221 cagtcaaatt cacagcgaat gcttcatctg ctttttgtgt tttcgcccct agaattgctc
    17281 gattcaaaat caagcggttg tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag
    17341 cctcacgata attgactttc aataaagata agtacatcac tgtatctaat tcatacaaga
    17401 caacaaatac atctgcacta ggtgcttctt cactttcttg gtagatatcg aaccaccgct
    17461 gaacgattgc ttcacttgct tccacaaaat tttcttgagc gattttaagt gattcaacga
    17521 tcatgctttc ttctgctagt tgtccagttt ttgtttgagc agaagagatt ttttggatct
    17581 tcttttgaag aaaatcacga atatattctt ttgtcagatc cagctctttt tgtgaaaaga
    17641 ctggagaacc agtttctctg tcaataatat gcagaattgc tttttttaaa tagatatcca
    17701 ttttctcatt cccttctgtc ttagttt
//
LOCUS       DAFCLG010000049        17444 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFCLG010000049 DAFCLG010000000
VERSION     DAFCLG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.049"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..187)
                     /locus_tag="LND56_001524"
     CDS             complement(59..187)
                     /locus_tag="LND56_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6743452.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="LND56_001525"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="LND56_001525"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6743453.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="LND56_001526"
     CDS             complement(1674..2729)
                     /locus_tag="LND56_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6743454.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="LND56_001527"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="LND56_001527"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBK6743455.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="LND56_001528"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="LND56_001528"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBK6743456.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="LND56_001529"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="LND56_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBK6743457.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="LND56_001530"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="LND56_001530"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK6743458.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="LND56_001531"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="LND56_001531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBK6743459.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="LND56_001532"
     CDS             10491..12050
                     /locus_tag="LND56_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBK6743460.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="LND56_001533"
     CDS             complement(12161..14092)
                     /locus_tag="LND56_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6743461.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="LND56_001534"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="LND56_001534"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK6743462.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="LND56_001535"
     CDS             complement(15021..15773)
                     /locus_tag="LND56_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6743463.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="LND56_001536"
     CDS             complement(15932..16141)
                     /locus_tag="LND56_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743464.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17378)
                     /locus_tag="LND56_001537"
     CDS             complement(16152..17378)
                     /locus_tag="LND56_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743465.1"
                     /translation="MIQPTFIRSFTQQADPYDLRKLQEKIMLASVRDESWLVVGNDEN
                     ECSFRLEDNQLLIKNVLSISVFKEKQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTK
                     QISQRLLFEKKDEIEHLPKMKQQNGEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYY
                     HQIIPKPIFLDIQQVEKVKELENEVICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHL
                     AWDNGVGLLKPPFVEYIYTDHTIQSVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKE
                     RRVRGTLNAQAYFPWVDENRSRMMCYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEK
                     YQEYLLSLRIYVPSESLSELPLKEIKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQ
                     FLDYRELEQLITLQKI"
BASE COUNT         4568 a         3775 c         3166 g         5935 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgag
//
LOCUS       DAFCLG010000050        17196 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFCLG010000050 DAFCLG010000000
VERSION     DAFCLG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.050"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            208..2250
                     /locus_tag="LND56_001538"
     CDS             208..2250
                     /locus_tag="LND56_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6743466.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="LND56_001539"
     CDS             2261..2881
                     /locus_tag="LND56_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK6743467.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="LND56_001540"
     CDS             2893..3351
                     /locus_tag="LND56_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6743468.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="LND56_001541"
     CDS             3370..3648
                     /locus_tag="LND56_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6743469.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="LND56_001542"
     CDS             3673..5040
                     /locus_tag="LND56_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK6743470.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="LND56_001543"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="LND56_001543"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBK6743471.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="LND56_001544"
     CDS             6227..6718
                     /locus_tag="LND56_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743472.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="LND56_001545"
     CDS             7161..8360
                     /locus_tag="LND56_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6743473.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="LND56_001546"
     CDS             complement(8855..9613)
                     /locus_tag="LND56_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6743474.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="LND56_001547"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="LND56_001547"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK6743475.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="LND56_001548"
     CDS             10713..12158
                     /locus_tag="LND56_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6743476.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="LND56_001549"
     CDS             12186..12641
                     /locus_tag="LND56_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6743477.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="LND56_001550"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="LND56_001550"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK6743478.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="LND56_001551"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="LND56_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="LND56_001552"
     CDS             14450..15064
                     /locus_tag="LND56_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6743479.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15305..15688
                     /locus_tag="LND56_001553"
     CDS             15305..15688
                     /locus_tag="LND56_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6743480.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16051..16560
                     /locus_tag="LND56_001554"
     CDS             16051..16560
                     /locus_tag="LND56_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBK6743481.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16660..>17196
                     /locus_tag="LND56_001555"
     CDS             16660..>17196
                     /locus_tag="LND56_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743482.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDW"
BASE COUNT         5503 a         2753 c         3629 g         5311 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttaggcg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaataacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatccaaca ataaaaatat ttttgtattt attagtatat
    15241 atactttaca aaaatttgta tatatactaa tttttttgta ggatcaatga aatgagggga
    15301 aaatatgcca aaatataaag aaatagcctt ggctctaaaa gagaggatcg ttgagggaaa
    15361 atatcaggaa ggttctaaat tacctgagca agaaagtctg gctagagagt ttaaaacaag
    15421 ccgtgttact atccgcaaag ctatacaact ccttattgat gaaggtttgt tatatacgag
    15481 acgtggatct ggtactttta tacgaagcaa tatcagacag gacaatgaga atgtcactca
    15541 agtaaataat gtatttggca cttcctcaca agaaaataca aatgtgacaa gtaaaataat
    15601 ccgttttgat gtccggtttc cgacagatta tgaaaggcgg ctttctgcaa cctacttccg
    15661 atccccttcc tgcaaatcat gggtataaaa aataggcata ttaagcgggc taggaggaga
    15721 aatattcaca tatcattttt tcattccgca agctgcgtca agttatttgg aatttgtaga
    15781 acagatgaga gaggtagaag cagcactaca aactactttt caataagtag gaattataga
    15841 aattttactc aaaaaatctt cattaaaaaa tctgactaaa taagttgaga aaacagaata
    15901 tacttcaata tccagtgatt tataaatatc attcactcat gatacccagc tccgtatcat
    15961 aagtgatgcg gtttttatca ttgatcctaa aaatcttagc aagacaaaaa gtacgtaaga
    16021 aagtaaagac taaatacttc ggaggaacaa atgaataaca atgataggtt attgcgattg
    16081 cgttatgcga ttgatttgaa agatacggat ttgattaaag cttttgagct aggcggtgta
    16141 ccactgacta aagaagaagc tcaggcggta ctgaccaaag tacaggataa gaacaaagat
    16201 aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc aagtttttga cgcattccta
    16261 aatggcctga ttctcttggc gcgtggtccg caaaaagata agccgatagt agacacgccg
    16321 caaaaaagca aggaaatcaa atatatcaac aatgtgttgc taaaaaaatt aaaaatcgcc
    16381 ttgtctatga ccactgatga tattttagaa gtttttgctg aggcagaact ctatccatca
    16441 aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac gaaattttaa gccttgcgga
    16501 gataaatata tgcgtaattt tctcaagggg ttaggaattt ataatcggag aaaagtataa
    16561 agaaatttca tgagaaatca aaacaatggt tgttttgatg agagctagtt tttgtatgag
    16621 attaaaatat tatcttaatc gaggagctgg attggtttta tgaatgatga atataagaat
    16681 gatgaagata agatgctttt tgaagagata gagaatcgat gtaggctcaa ttttgagctt
    16741 cggggaaaga tgtctttaat acaacaaaaa aagtatttag cgaataaatc agagtttact
    16801 ctgggacatg ttgaaaaatt aatctcggat tggattagta gtagaagcga atttacaaaa
    16861 ataaaacagc cgattaaatt tgacatgaaa aaacttttgt taaataagag tgagataggt
    16921 aatagagatc aatatattag agctaaaggg caagaaataa ttgattcatt aggtgaagtg
    16981 aggtcataca attatttgta tgtaacacat agagctgatg gaatggtcat tactgtgggg
    17041 aaatcttctt ctaatgatat ttttttagat ggagatcttt tttatcaact caatacaaat
    17101 cacttgtctg gcactgaaaa tataatttta aggacggaat atggcaatga aatatttgca
    17161 aaatatgatg agattttaaa aaattatttg gactgg
//
LOCUS       DAFCLG010000051        17180 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFCLG010000051 DAFCLG010000000
VERSION     DAFCLG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.051"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="LND56_001556"
     CDS             complement(543..2048)
                     /locus_tag="LND56_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6743483.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LND56_001557"
     CDS             complement(2045..2731)
                     /locus_tag="LND56_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743484.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LND56_001558"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LND56_001558"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK6743485.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="LND56_001559"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="LND56_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK6743486.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="LND56_001560"
     CDS             complement(4752..5321)
                     /locus_tag="LND56_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBK6743487.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="LND56_001561"
     CDS             5507..6409
                     /locus_tag="LND56_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6743488.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="LND56_001562"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="LND56_001562"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK6743489.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="LND56_001563"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="LND56_001563"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK6743490.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="LND56_001564"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="LND56_001564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK6743491.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="LND56_001565"
     CDS             12977..13162
                     /locus_tag="LND56_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBK6743492.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="LND56_001566"
     CDS             complement(13224..14420)
                     /locus_tag="LND56_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6743493.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="LND56_001567"
     CDS             complement(14590..16026)
                     /locus_tag="LND56_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6743494.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="LND56_001568"
     CDS             16319..17128
                     /locus_tag="LND56_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6743495.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5042 a         3595 c         2983 g         5560 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct
//
LOCUS       DAFCLG010000052        17101 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFCLG010000052 DAFCLG010000000
VERSION     DAFCLG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.052"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..1421
                     /locus_tag="LND56_001569"
     CDS             48..1421
                     /locus_tag="LND56_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6743496.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            1421..2347
                     /gene="dnaI"
                     /locus_tag="LND56_001570"
     CDS             1421..2347
                     /gene="dnaI"
                     /locus_tag="LND56_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK6743497.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            2476..2916
                     /locus_tag="LND56_001571"
     CDS             2476..2916
                     /locus_tag="LND56_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK6743498.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3060..3761
                     /locus_tag="LND56_001572"
     CDS             3060..3761
                     /locus_tag="LND56_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBK6743499.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            3758..4879
                     /locus_tag="LND56_001573"
     CDS             3758..4879
                     /locus_tag="LND56_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBK6743500.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            4894..6126
                     /gene="pepT"
                     /locus_tag="LND56_001574"
     CDS             4894..6126
                     /gene="pepT"
                     /locus_tag="LND56_001574"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK6743501.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6258..7166
                     /locus_tag="LND56_001575"
     CDS             6258..7166
                     /locus_tag="LND56_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBK6743502.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7201..7473
                     /locus_tag="LND56_001576"
     CDS             7201..7473
                     /locus_tag="LND56_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBK6743503.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            7484..8530
                     /locus_tag="LND56_001577"
     CDS             7484..8530
                     /locus_tag="LND56_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBK6743504.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            8781..11390
                     /gene="clpB"
                     /locus_tag="LND56_001578"
     CDS             8781..11390
                     /gene="clpB"
                     /locus_tag="LND56_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK6743505.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(11541..12227)
                     /locus_tag="LND56_001579"
     CDS             complement(11541..12227)
                     /locus_tag="LND56_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743506.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(12205..12699)
                     /locus_tag="LND56_001580"
     CDS             complement(12205..12699)
                     /locus_tag="LND56_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6743507.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(12719..13939)
                     /locus_tag="LND56_001581"
     CDS             complement(12719..13939)
                     /locus_tag="LND56_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6743508.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14086..15921
                     /gene="lepA"
                     /locus_tag="LND56_001582"
     CDS             14086..15921
                     /gene="lepA"
                     /locus_tag="LND56_001582"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK6743509.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(16015..>17101)
                     /locus_tag="LND56_001583"
     CDS             complement(16015..>17101)
                     /locus_tag="LND56_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6743510.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         5629 a         3111 c         3574 g         4787 t
ORIGIN      
        1 agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg gaaaatgctt
       61 ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg ctgagagaag
      121 acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa gccttgagta
      181 tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct gaagggatgc
      241 atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat gcaagaaaaa
      301 agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat ctgggacctc
      361 gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa gactctgttc
      421 tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt acccaattct
      481 ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa tttttagatg
      541 tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa tcgctgaata
      601 aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca ttcgattggc
      661 agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat ccaatcatca
      721 aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt ggcgaactga
      781 tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg caaaaagcca
      841 ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg gtttctgaaa
      901 acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag ctgataaaag
      961 aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa aaaggcggat
     1021 atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca ggtctttcca
     1081 gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat gctagcttaa
     1141 atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc gccacagcag
     1201 aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca aaacaaaaga
     1261 agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt cctgattggg
     1321 tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag attgaccgcc
     1381 gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg taggcaaaga
     1441 attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa tgatcgctga
     1501 agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag aattgacaca
     1561 agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa agcgtaaata
     1621 tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga cgttgaattt
     1681 tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc aaagacaaga
     1741 agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat cggcttcttt
     1801 tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc tagattttgt
     1861 agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag taggaagttt
     1921 tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga ttgctggatt
     1981 caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg caatcggaaa
     2041 agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga tgatcgatga
     2101 cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg ttatccttca
     2161 atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa tggatgaatt
     2221 ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag cgaaacggat
     2281 aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca accgacgcaa
     2341 tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc aggtcagcga
     2401 atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg cagaaactta
     2461 aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg tctatttttt
     2521 cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc gattgatgcg
     2581 ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt tgtcggaacg
     2641 attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa actcgtcgat
     2701 gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga cggagtgatc
     2761 ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat cggtgacaaa
     2821 gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc gcaagttcct
     2881 ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt tcaaaaataa
     2941 gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg gaattttttt
     3001 attcctagtc tccttatttt atttggaaaa tgctataatt atctagaagg agtgaagcta
     3061 tgaaccattt agatttatct cagcgcttag ccgcagtggg tgactttgtc cctgaatccg
     3121 ctcgtttagc agacattgga tcagatcacg cttacttacc cgttgcattg atattaagag
     3181 gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg tccttatgaa tcagcacgta
     3241 agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt acgccttgca aatggtctag
     3301 atgctatcca gccacaagat ggtattacag ctgtcacgat tgcgggtatg ggaggttcac
     3361 tgatccgaga tatcctagaa gctggcaagt tgaatcaacg cttaagcgga caagaacgtt
     3421 tgatccttca gccaaacatc ggagagcgta cattacgatc ttggctgcaa gaaaatcagt
     3481 ataaaatcca atcagaaaaa atacttgaag aaaataaaaa aatatatgaa atcattgtag
     3541 ccgaaaaaga acaaaaacca gttacttaca atgaaatgga gctgatgttc gggccatttc
     3601 tgcttgaaga gaaaagtgag atcttcaaat caaaatggac aagagagctt caacaaagaa
     3661 aagctatccg tgaacagctc aaacaagcag ctaatcaaga ggcacgtcaa aaacaaatcg
     3721 agcaggaaat ccaaatgatt gaggaggttt taaagccatg acaatcagcg gacgcacttt
     3781 catccaaaag tttgaaagct attgtcctca gtggctagct gaagaaggag acccagtcgg
     3841 actgcatatc ggaacattgg ataagccaat ccaacgagta atgatgacat tagacgttcg
     3901 tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat ttgctgattg ctaaacatcc
     3961 tccaatcttt cgtcctgtaa aacggctggt tactgatcag ccacaagaaa aaatgtatgc
     4021 agatttgttg aaacatgata ttgccgtcta tgcagcacac acgaacatgg atattatttg
     4081 ggatggactg aatgactggt tttgtgaact tctcggtatt gaagtggaga gttatttagt
     4141 aaagactcat gagattcatt ataaaaaact ggccgtttat gtacctgtgg atcatgcaca
     4201 gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca caaggtgatt ataccggtac
     4261 aagttttacc tcgattggac acggtcgatt tactccggaa gctggtgcac aaccagcgat
     4321 tgggaaagta gggaagacag aacaagttca agaggcaaaa gttgaggtta ttttgccgga
     4381 aactatcgaa aaacaagtga ttcaagccat gcggtcagct catccttatg aggaacctgc
     4441 ttatgatctt ttcgctatag atgaaccagt cgaaatgttt gggttaggac gagtaggtga
     4501 attgccgcaa gaaatttcta tagaggcatt tgtagaacaa gtaaaagaag cgttccaact
     4561 agatggactg agaatagtcc agccaaaaaa tgcaaaatcc tctgtgaagc gcattgcgat
     4621 ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc gcccaaagag cagatgtcta
     4681 tatcacggga gatatctatt atcatacggc tcatgatatg caaagcgctg ggttgattgc
     4741 aattgatccc ggtcattata ttgaatcgtt atgcaagcaa cgatttatcg agaaatttga
     4801 atcttggaaa caagaagaaa attgggatat taactttttc gtttctgaaa caaatacaaa
     4861 cccatttcaa tttaattaaa aggagagaat actatgtacg aaaatctttt gccacgtttt
     4921 ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa gcaaaacgac tccatcgact
     4981 cagacacaag ttgcttttgc gcaagaattg aaaaaagaat tagaagagtt aggcttagct
     5041 gatgttttct ataatgaaga taacggattc gttatgggaa cattaccaag caatgttgat
     5101 catgaagtgc gttccattgg atttatttcc cacatggata cagcagattt taatgcagta
     5161 ggagtcaatc cacaaatcat tgaaaactat gatggcgagt ctacgatcgt actagatgcg
     5221 gaagaacgct atacattgaa tacaaaagat tttccaaatt tgaaaaatta tgaaggccac
     5281 acattgatca caacagatgg cagtacatta ctgggagcag atgacaaatc agggattgcg
     5341 gaaatcatga ctgcaatgga gatcttgatc aataatcctt ctatccctca cggtaccatc
     5401 aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag cggataaatt cgatgttgag
     5461 caattcgatg tagattttgc ttatacagtc gatggtggtc ctttaggtga gttgcagtat
     5521 gaaacgttta gtgctgctca agcaaatatt acgatccaag ggaaaaatgt acatccaggg
     5581 acggcaaaag atacaatgat caatgctcta cagttagcaa tcgattttca taatcagctt
     5641 ccagccgatg aagttccaga aaaaacggaa ggatacgaag gtttcttcca tttgatggca
     5701 ttggatggga atcctgaaga agcttctatg agttatatca ttcgcgatca tgatcgtgaa
     5761 aaattcgaag cacgcaaagc acaaatcaca catatccaag aagagttgaa tcagcgattt
     5821 gatcaagaac gaatcaaagt agatatgtat gatcaatatt acaacatgaa agaaatcatt
     5881 gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa tgatcgagct tggtatctct
     5941 ccagtgatcg aaccagttcg tggaggaaca gatggttcaa aaatctctta catgggtatt
     6001 ccgacgccaa atctatttgc cggaggagaa aatatgcacg gacgattcga atttgtttct
     6061 ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa ttgcagaatt gaacacaaag
     6121 agttaaaatc gaaaaagaac agcttatgac aaggcgttcg tcccaatgat ggaatgtcat
     6181 ctgtctaagg ctaaagggca ggacaatgga attggttgtc ccgccctctt ttttgaagag
     6241 taaataggag gtaaagaatg gaaaagctgc tagtttttta taacaaaagt tcagggaaag
     6301 atgagggtga gaaattagca cgctggtttc aagattatgt aaaagaaaag cagccagaat
     6361 tatatgtaga actagctgaa actggcccat cgattgatga tgcggaatta gtcaagaaag
     6421 cagaagccat agaagctgat actttagtaa ttataggtgg agacggcacg attcatcata
     6481 tcgtccaagc gttccaaaaa acattatctc aataccatgt cggattactt ccaggtggaa
     6541 ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa aaaagaagaa gctgctgacg
     6601 tgatcctgga aggcaatgta cggaaaatag actatggtca agtcaatgat gaagtcatta
     6661 tctctacact gacaatcgga atcttagcag atacagcagt ttggatcagt caagaagaaa
     6721 aacagcgtta cggctcgtgg atctttatca agaagttttt taagttattg ctgaagaaga
     6781 aaaaatatca tttggattta gaaacagacg aggagcattg gaaaggaaaa tcccaactgt
     6841 tgacagtcac gatgtctaac tccgtgggcg gttttacaaa ttttgacgac tcagcgaccc
     6901 tagatgatgg aaaatttcat gtaacgatcg taccttcctt agatattttc cgatttattt
     6961 tttatttgcc tagaatcatg agaggcaaat tctatacgat ccccggcatt acttatttca
     7021 cagccaaacg catgaaaatc caagcgcagg aaaaatctgt gggcactcga acggatggag
     7081 acacgacaga tgatcttcca gttgaattat ctgttgtatc aaaaggatta acagtttatt
     7141 caccaaagaa agaagaaaaa gcttagaaat agtagttaac agaaagttgg aagaagagta
     7201 atggatgtat tgttacacaa tcctttgtgg ttttaccttt tcattgtgaa tttttactta
     7261 ttttgtctga tggggtatga taaacatcag gctagaacta gcggctggcg tgttcctgag
     7321 aataatttga tatttgtcag cctgttaggc ggtggtttgg gaggattgat ctctcgaaaa
     7381 gttttccatc ataaaactcg gaaaaagaag tttactatag gattcgctgc aggtgtactt
     7441 gttgatttga ttctcattta ttttttccat taaggagcag gagatgtcgt ctaaaggaac
     7501 gaaaactaaa atcattctga atctgctttt gctgattgtg atttttgcca ttatttatta
     7561 tttaatcaat caatcatttg cagatatctt tgccgaattg ctgtctacca gtgttgttgt
     7621 tttggcagcg gctgttatat tagggacagt ttaccaaatc gctgaagggc gctcaatcaa
     7681 agaaattgct gaacctttcg cgccgaattt tacaacgaaa gatgggttct tgacctctag
     7741 ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga acgctgatct cggagattta
     7801 tttttataac aaaaaaggaa tgaagctatc ccagggagca ggggtgacag cactccatat
     7861 gattatgtac aaactggcag tgatcactta tgcattagct ggcttggtca tccaattctc
     7921 tcttttttat tctaatggtc catcaatgat ctggtttatc atcgctggta tccttttgac
     7981 gatcgtgatt gttgcctttt tattggcgat gtccgtcagc ttgaatgttc aaatattttt
     8041 tgtcagctta tcgaataagt tatttaaaag tgagcgtttg cggaaatggg tggatacatg
     8101 caatcagcag atctactctt taagggaaac ggtccaaacc attatccaag acaggactgc
     8161 gctattgcgg atttatctat ggaatatgtt aaaacttgta ttttggtatg tacttcctta
     8221 tttgattctg attgaaaacc atcctaaaat agattttcta ctgaccattt cgtttatcag
     8281 ctttgcagta gtgctttcag gggttattcc tacgcctgct ggtattggtt cctttgaatt
     8341 tgtctatttg ttattattca gaccagttgt tggaacagtt gacgcagttt cttcgctttt
     8401 actgtatcgt ttcagttcct ttatcttgcc atttctctat ggatttgtgt atgtgattgc
     8461 tgaacggcga agagtgatca aagaagaaat aatagaagta aaaaaagaga ataaagaaaa
     8521 tcaagggtag gtcttttata agatctaccc tttttatcaa atcaaagaaa aacgttatgg
     8581 ataagataat tttacgtttg tttggctctt tttttatata catacaatca attttgtttt
     8641 tttaagattt atataagtcg agaaaaaaag tcaaaaaact atgtgtatat tttgaccaaa
     8701 attgaccaaa gcgatataat agtttttgta aagatcaaag aaataagtga tcttaccttt
     8761 aattaggagg gtatacctgt atgaatattg agaaaatgac tacgacgctt caagaagcga
     8821 ttgctgaagc tcaacagatt gcggtcactc gaaaacatca agatatcgac attgcccatg
     8881 tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa tttctatacg gatgccggat
     8941 tggatgtcga atcttttgaa catgaaattg atcgtctact ggatgagtat ccagttgttt
     9001 ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa tttctttcga ttattgaatg
     9061 aagcagacca aatacgtgaa agcttcggcg acgagtttct ttcaacggaa gtcgttattt
     9121 tagcgttgat gaaattaaaa aattatccgt taactgttta tttgaataaa aacggattat
     9181 ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg aggagaaaga gtgacctcac
     9241 aaaaccaaga agaacaatac aaagcattag aaaaatacgg cgttgattta gtacagcaag
     9301 tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt gatgtcatcc
     9361 gtattctttc acgaaaaaca aaaaataatc ctgttctgat aggagaaccc ggtgtaggga
     9421 aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg cctgaaaatt
     9481 taaaagataa aacgattttt tcattagata tgggtgcatt gatcgctgga gcaaaattcc
     9541 gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa agtgatggac
     9601 gtatcctctt gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa acagaaggaa
     9661 gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta catttgatcg
     9721 gtgccacaac tttagatgag taccgtcaat atatggaaaa agacaaagcg ttggaacgtc
     9781 gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga tacgatcagt attctgcgtg
     9841 gattaaaaga acgttttgag atccaccatg gtgttaacat ccatgacaac gcattagtag
     9901 cagcagctac tttatctgac cgttatatta ctgatcggtt cttaccggac aaagcaatcg
     9961 atttgatcga tgaagccagt gcaacgattc gggtagaaat gaactctatg ccaacggaat
    10021 tagatcaagt tactagacgt ttgatgcagt tggaaatcga agaagctgca ctgaaaaaag
    10081 aatcagatga tgcaagtaaa aaacgcttga aaaatttgca agaagaatta gctgaacttc
    10141 gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa gtcaattctg
    10201 tttcagcaaa acgtgctgaa atcgataaag caaaacacga attagaagat gcagaaaata
    10261 actatgattt agaacgcgct gctgtattgc gtcatggtac gatacctcaa ttagaaaaag
    10321 aattgaagga attagaagct aaagcaaaag acagcgagat taagatggtt caagagtccg
    10381 ttaccgaaaa tgagattgct caagtcgttg gcagattaac gggtatcccc gtaacgaaac
    10441 ttgtagaagg tgaacgagaa aaactaatta aattgaatga aacacttcat aaacgagtga
    10501 ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt aattcgttca cgggctggat
    10561 tgcaagaccc aaatcgtcct ctaggttctt tccttttcct aggaccaact ggtgtcggaa
    10621 aaaccgaatt agcgaaagcg ttagctgaga acttgtttga ctcagaagat catatggtac
    10681 ggatcgatat gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc ggagctcctc
    10741 caggctatgt aggttacgaa gaaggtggtc aattaacgga agctgtcaga cgaaatcctt
    10801 atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc aatatcttgt
    10861 tacaagtcct agatgacgga cgcttgactg attcaaaagg gcgagtagta gatttcaaaa
    10921 atacggttct gatcatgacc agcaatatcg gttcacagtt actgctggaa ggcgtgactg
    10981 ctgatggaac gattccagaa gcagtggctg aacaagtcaa cacgctatta cgtggaaact
    11041 tcaaaccaga attcttgaac cgaatcgacg acacgatttt atttacacca ttgagcctag
    11101 acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt ctggaacatc
    11161 aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa aatgcttatg
    11221 aaccagcata tggtgcaaga ccattgaaac gatttatcac taaggaagta gaaacaccac
    11281 tagctaaaga aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg attactttac
    11341 ttgatggaca acttcatttc aaaacagaag agctagaaga aatcgtctaa tcttccattg
    11401 agactataaa agaaaaaagg ggtctgacac aactagtgtc agacctcctt ttttcaaata
    11461 aacagtgttc cagaagtagt ttcttcgaaa aataagccga aatttcttga agctgcccgc
    11521 ttctgtcaca acctctttta ttaggtattt ttcacttttt ctaatttata actttcaaag
    11581 taacgctcat cttttttact ttgagaccat gcaaacaatc ccattaaggc agcacctatc
    11641 gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac aaacagtgtg
    11701 ccatcagaag cagcaaaacg ttgaagattc atgcctaaga actggtcgca taagaattcc
    11761 cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa gaggaacaac
    11821 caaggactgg tttttgatat ctcagcatct ttcatcaagt aaccaatcaa tccatagcca
    11881 agagctgtca ggaccattgg actcaccgca tgtaagattt tgtcccaaaa agagatgata
    11941 ctgatcaaat gcatccctgt gcctaaaaag acagagatga acaagaaaaa ccagtaaaac
    12001 aaaacgattg ctgctggtat tttaagtttg aaaactttca aaatgacttg tggcatgaaa
    12061 atcaatgcaa tccctaataa agattccacg ataaatgtga ttccttgaga agtcgaatat
    12121 ttttgtgtga taaattcata aaaattatag cagacaacga atacaccaaa agcaagtaaa
    12181 gctatttgca cctttttgat ttctttaatc actgaaagtc cctccatcta aaaatctttt
    12241 tacttctgaa agcaactcaa tttctgaaga aaccactttt ccgttcaatt tgatcaaacc
    12301 tattgtatat aagttcaggt agtgaaattg attttcagct gtttcttgta gtgcagcgat
    12361 ttttttcggg tgggttccgc cttgctgtcg agtatcagta tataagccga tcactgggat
    12421 gcctttcgca taagccacac cgatttccga tgctaccccg gcatcgatcg tcagcccatc
    12481 tagtaaagca atcattaaat cactagccaa cactttttct gtgtcagcaa gagcaatcat
    12541 tcgactgtct gcataagcag ttttgtcatt gattgcttca ttttcttgag gaagatagat
    12601 cgaaagctca gaagagagct ctctgatttc tttaactaac tgttggttat ataacaaatc
    12661 acttttagca aacattggtg cggcaaaata aattttcatc taaacatcat cctttttatc
    12721 aaaaataatc gctaaagtaa gcttccatgt ttggaatgat acgctctagt gacttcagtg
    12781 tttcttcatc tgtttctaac cgttcgattc ttgccaaata agaagcacgg cgataattca
    12841 tcatcaagca agtcaatgtt tggatatcca gtcgaacgga ttttcctata ggttcattta
    12901 agacacggac ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta ttccaaggag
    12961 cgacaggatc ttcaatgatg aaatgaaaag catcagctgt tccaacaaac ggaaaccgtt
    13021 gaaggaattc ttttacatca acgatccgag ccataaaata tggttcgatc tgctctttga
    13081 tctgactatc ttcaatcaaa aatgctaaag gctcattttt ataaatatct ccatgtaccc
    13141 agtagatcat ggaaaaatgt gcggaaataa agttccataa gccatttcga gcttcttgat
    13201 caagatagaa catttctttt acgtgaaata tttcttcagc tacccagtag aacagaacac
    13261 ctcttggttc atggttgcta tcgtagtaga cagcggctgt gcgctcatct tcattttcga
    13321 aacgccaata ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg ttttgttggg
    13381 caaatttgtc ataaacggaa aatacgtctg gatgatcaac tggtaatcgc tcaaccatac
    13441 cacttactgg gatttgttta ggtagctgtg tatctctgat tttaaaggaa agtttatcag
    13501 acatgatttc ccatccttta cgccgatagt aggggatact gtaaggatac aaataagaga
    13561 tccattgctt attcttgcgc atattatcca atgcaacgat aattagatct tgcatcaatc
    13621 catggttagc atattctgga taagttccaa ctccagtcac accacccatt ttataacgtg
    13681 caccgtgaat attgacttca caaggataga tagcgatttg agagatcagt ttattttcgt
    13741 ggaaccatcc gaacactttc gatacttcca aaatcggttt tttggaacgg ataaaagctc
    13801 gtttattctc aaacccgctt tcctcgatgt ctgcttcagt cacttggaac acataggaaa
    13861 gcaattcatt gaattgatcg atatgttctt gagtgaccgg ttttaattca agattttctc
    13921 taaaatcttt ttgatccatt ttttcatctc catttattag cttgataaca gtatagcaaa
    13981 attctacaaa aaaaacttct atagctgact aattaaaaac tctttaaatt actgcttttc
    14041 gttggtataa tggaaagtgc tgattatatg aaaagaggag acgctatgga tataaagaaa
    14101 atgaaagaac gccaagagca gattcgtaat ttttcgatca tcgctcatat cgaccatggg
    14161 aaatctacat tggccgatcg tattttagaa atgacgcaca ctgtgacgtc tcgcgaaatg
    14221 caagatcagc tgttagactc gatggatttg gaacgtgaac gtggaatcac gattaaatta
    14281 aatgctgtag aactcaatta cacagcgaaa aatggagaaa catatatctt tcacttgatt
    14341 gacaccccag ggcacgtcga cttcacctat gaagtctctc gaagccttgc tgcctgtgag
    14401 ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt ggccaatgtt
    14461 tatttagcgt tagacaatga tttggagatc ctgccagtca tcaataaaat cgacctaccc
    14521 gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat cgatgctagt
    14581 gaagctgtat tagccagtgc caaatcaggg attgggattg aagatattct cgagcagatc
    14641 gtggaatatg ttcctgcacc aagtggagac ttagatgcac cgctaaaagc tttgatcttt
    14701 gactctattt atgatagtta ccgcggtgtt gttctaaatg tccggatcac ggacggtatg
    14761 gtcaaacccg gagacaaaat caaactgatg agtaatggca aaacatttga tgttacagag
    14821 gtcggggtct tctcgccaaa agcagttgct agagactttt tgatggtcgg tgatgtcggt
    14881 tatatcacag caagtatcaa aactgtccaa gatacacgag taggggatac agtaacgtta
    14941 gcagacaatc ctgccgctga agctttacca ggttaccgaa aaatgaatcc gatggtttat
    15001 tgtggtcttt atccaattga tacttctcgt tacaacgatt tgcgggaagc tttagaaaaa
    15061 ctgcaattga atgatgctgc tttacaattc gaaccagaaa cctctcaagc attaggtttt
    15121 gggttccgct gtggattcct cggacttctt catatggatg tcgtccaaga acgcttggaa
    15181 cgggaattca atctagaatt gatcacgact gcaccatccg ttatttacca tgttaataaa
    15241 acggatggta cgactgctac agtagataac ccggcagatt ttcctgaacc tgtaacgatt
    15301 caggatgtag aagaaccatt cgtcaaagca cagatcatgg tcccaaatga ttttgtcggt
    15361 gcagtaatgg aactttctca acgaaaacgg ggagaattca ttacgatgga ttatttagat
    15421 gattaccgag taaatgtcgt ctataatatt ccgctttctg aaattgtctt tgactttttc
    15481 gacaaattga aatcaagcac gaaaggctat gcctcattgg attatgaaat gtctggttat
    15541 caaaaaagca agttagtgaa gatggacatc ttgctgaatg gagaaaaagt cgatgcatta
    15601 agctttatcg ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt tgaaaaactg
    15661 aaaaaactaa tcccacgcca acaatttgag gtgccaatcc aagcagctat tggacaaaaa
    15721 atcgttgccc gttcagacat caaagccttg cgtaaaaacg tcttggctaa atgttatggt
    15781 ggagatgttt ctcgtaaacg taaactctta gagaaacaaa aagaagggaa gaagcggatg
    15841 aaacaaatcg gctccgtcga agttccgcaa gaagccttca tggccgttct gaaaatggac
    15901 gaggacgaac cgaagaaata gcaagggttt gtttgaaaac agtgtaaaaa ataaaccatg
    15961 tttacatgga tttgccaccg gtttgccacc ggtggttttt ttcggtggca aaaatcaaga
    16021 attctttcta agattttcca tgatatcgac cgtttcattt ctcatcttat ctgttacatg
    16081 cgagtaagta tccatcgtaa ttgaaattct gctgtggccc aatcgttcag agatttcttt
    16141 catttttgcg ccattttcga gaagaagtgt agcatgagta tgtctgagag aatggaagtt
    16201 aaaagagagg gagagtgcat tcgatatcct cctagtattc catttcacta cacttggcgt
    16261 aactagctca ccgtcttcct tcgtacatac tgcatttgaa tcaatgtaga gctttccata
    16321 cttcattcga ttttctaatt gttgtttctt atgttttttc agaattgcta gcaaggtttg
    16381 tccaataaaa atcgttcgat tggaactgct tgtctttggc gtaccatata cccatgcgcc
    16441 gtcattcttt accatttgtt tctctacagt aattgttcca tttgaaaaat cgacatcatc
    16501 ccacgtcaga ccacaaactt cgccaacgcg cattcccgta taaaatccaa tattcaaagg
    16561 aatatagaaa ggatgtcctt caggagtaat ttctagcata tgatcaaagt cctcaagaga
    16621 aatgattttt agatcttttt tagtcgttgg tcgtgcttca tattttggta tttttacata
    16681 cagcatagga ttttgcttga ttaatcccca aggataaacc gccatattca gcgcattctt
    16741 aaggacagag tgagtaatag tcattgtttt cttcgagtaa ccctttttaa attcagcatt
    16801 gatgaaattt tgtaaaagag caggggagag atccgtaagt tttttctttc ctaaataacc
    16861 gtttatatga tttttgatgg taaatcggta gttttcatag gtattgtatt ttagatttag
    16921 tttaacgtat tcctccatcc aaaaatcaag gtattgtttt actcgagtat ccgtacctaa
    16981 aaagtattgt cctgtttcgt caatatctga taaaactttt cgtaaagcag cttcggcctc
    17041 tggtcgggtg tctccgccaa ctttctccac tttttttctt gagccatcat cattgatatc
    17101 t
//
LOCUS       DAFCLG010000053        17008 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFCLG010000053 DAFCLG010000000
VERSION     DAFCLG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.053"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..127)
                     /locus_tag="LND56_001584"
     CDS             complement(<1..127)
                     /locus_tag="LND56_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBK6743511.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="LND56_001585"
     CDS             complement(231..542)
                     /locus_tag="LND56_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6743512.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="LND56_001586"
     CDS             complement(544..741)
                     /locus_tag="LND56_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBK6743513.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="LND56_001587"
     CDS             complement(1135..2862)
                     /locus_tag="LND56_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBK6743514.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="LND56_001588"
     CDS             complement(2855..4048)
                     /locus_tag="LND56_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6743515.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="LND56_001589"
     CDS             complement(4235..4879)
                     /locus_tag="LND56_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6743516.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="LND56_001590"
     CDS             complement(4973..5107)
                     /locus_tag="LND56_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBK6743517.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="LND56_001591"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="LND56_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBK6743518.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="LND56_001592"
     CDS             complement(7238..7711)
                     /locus_tag="LND56_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBK6743519.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="LND56_001593"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="LND56_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK6743520.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="LND56_001594"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="LND56_001594"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK6743521.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="LND56_001595"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="LND56_001595"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK6743522.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="LND56_001596"
     CDS             complement(11758..12897)
                     /locus_tag="LND56_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6743523.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="LND56_001597"
     CDS             complement(13206..13919)
                     /locus_tag="LND56_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK6743524.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="LND56_001598"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="LND56_001598"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK6743525.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="LND56_001599"
     CDS             15108..16640
                     /locus_tag="LND56_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6743526.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="LND56_001600"
     CDS             complement(16831..>17008)
                     /locus_tag="LND56_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6743527.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3496 c         2910 g         5323 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ccggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DAFCLG010000054        16993 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFCLG010000054 DAFCLG010000000
VERSION     DAFCLG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.054"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="LND56_001601"
     CDS             <1..1152
                     /locus_tag="LND56_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBK6743528.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="LND56_001602"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="LND56_001602"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK6743529.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="LND56_001603"
     CDS             2728..3003
                     /locus_tag="LND56_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBK6743530.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="LND56_001604"
     CDS             3009..3776
                     /locus_tag="LND56_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBK6743531.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="LND56_001605"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="LND56_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBK6743532.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="LND56_001606"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="LND56_001606"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBK6743533.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="LND56_001607"
     CDS             7920..8222
                     /locus_tag="LND56_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBK6743534.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="LND56_001608"
     CDS             8225..9388
                     /locus_tag="LND56_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6743535.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="LND56_001609"
     CDS             9414..12842
                     /locus_tag="LND56_001609"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBK6743536.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="LND56_001610"
     CDS             12863..13987
                     /locus_tag="LND56_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743537.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="LND56_001611"
     CDS             13977..14414
                     /locus_tag="LND56_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6743538.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="LND56_001612"
     CDS             14398..14736
                     /locus_tag="LND56_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBK6743539.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="LND56_001613"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="LND56_001613"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBK6743540.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="LND56_001614"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="LND56_001614"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK6743541.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="LND56_001615"
     CDS             15893..16936
                     /locus_tag="LND56_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBK6743542.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5752 a         2944 c         3750 g         4547 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcg
//
LOCUS       DAFCLG010000055        16709 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFCLG010000055 DAFCLG010000000
VERSION     DAFCLG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.055"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(146..1111)
                     /locus_tag="LND56_001616"
     CDS             complement(146..1111)
                     /locus_tag="LND56_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBK6743543.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="LND56_001617"
     CDS             1345..2190
                     /locus_tag="LND56_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6743544.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="LND56_001618"
     CDS             2381..3568
                     /locus_tag="LND56_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6743545.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="LND56_001619"
     CDS             3574..3930
                     /locus_tag="LND56_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBK6743546.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="LND56_001620"
     CDS             3932..4270
                     /locus_tag="LND56_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBK6743547.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="LND56_001621"
     CDS             4665..5699
                     /locus_tag="LND56_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6743548.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="LND56_001622"
     CDS             5692..6378
                     /locus_tag="LND56_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743549.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="LND56_001623"
     CDS             6402..7250
                     /locus_tag="LND56_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6743550.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="LND56_001624"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="LND56_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK6743551.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="LND56_001625"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="LND56_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK6743552.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="LND56_001626"
     CDS             9516..10751
                     /locus_tag="LND56_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6743553.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="LND56_001627"
     CDS             10738..11208
                     /locus_tag="LND56_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBK6743554.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="LND56_001628"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="LND56_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK6743555.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12699..13841)
                     /locus_tag="LND56_001629"
     CDS             complement(12699..13841)
                     /locus_tag="LND56_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6743556.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13880..14614)
                     /locus_tag="LND56_001630"
     CDS             complement(13880..14614)
                     /locus_tag="LND56_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743557.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            14846..15121
                     /locus_tag="LND56_001631"
     CDS             14846..15121
                     /locus_tag="LND56_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743558.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15219..>16709
                     /locus_tag="LND56_001632"
     CDS             15219..>16709
                     /locus_tag="LND56_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743559.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKI"
BASE COUNT         5494 a         2966 c         3500 g         4749 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12661 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12721 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12781 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12841 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12901 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12961 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    13021 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13081 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13141 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13201 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13261 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13321 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13381 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13441 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13501 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13561 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13621 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
    13681 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
    13741 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
    13801 acttgaccgt ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt
    13861 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
    13921 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
    13981 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
    14041 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
    14101 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
    14161 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
    14221 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
    14281 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
    14341 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
    14401 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
    14461 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
    14521 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
    14581 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
    14641 tacctcaaaa aaggggtgtt gtaaataaat tttacaaagc tgttgacacc ccttttttat
    14701 cgaggtatta tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa
    14761 taacaaaata cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca
    14821 agcaatcacg aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat
    14881 acaagttgaa caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat
    14941 tatttctgga gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt
    15001 atcagtagga acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga
    15061 tacggtcatt agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg
    15121 agtaaacaaa taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt
    15181 tttagtcttt aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata
    15241 ttccaattta tgtagatcaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa
    15301 ctgatgtaag agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag
    15361 agatactaaa ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca
    15421 aggagagttt tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg
    15481 agaaggggaa ggtagtagat ttgagtaaag atgatagtag atacttaagc atagaaaagg
    15541 ttttatctat caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact
    15601 ctgaaaatct agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg
    15661 gtaaaatggt tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg
    15721 taaatgcttc gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta
    15781 atgaagacaa catgttagat attactgagc ttccgattga ttttaccatg acatgttcta
    15841 aaaattgtca aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta
    15901 agttgataaa aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg
    15961 attcctccga catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata
    16021 aattacttaa gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg
    16081 ctagtatttt taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg
    16141 atatcagtga aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag
    16201 aaacatttaa ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga
    16261 aagaggaatc aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga
    16321 caataaggaa ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta
    16381 atttttttga tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta
    16441 aaaaaatgag ttcttactat ggtaaaactt atgacataga taaatgtaat aagttactat
    16501 cacttatgac ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga
    16561 tgctcgaata cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aatcataaga
    16621 gaagcaatgt ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaatg
    16681 aagatatcct tccacttctg gaaaaaatt
//
LOCUS       DAFCLG010000056        16039 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFCLG010000056 DAFCLG010000000
VERSION     DAFCLG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.056"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..641
                     /locus_tag="LND56_001633"
     CDS             <1..641
                     /locus_tag="LND56_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743560.1"
                     /translation="KNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGKSTLLSL
                     LAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIRLVNKSA
                     DESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAG
                     EIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            641..2017
                     /locus_tag="LND56_001634"
     CDS             641..2017
                     /locus_tag="LND56_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743561.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2303..3925)
                     /locus_tag="LND56_001635"
     CDS             complement(2303..3925)
                     /locus_tag="LND56_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBK6743562.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            4577..5548
                     /locus_tag="LND56_001636"
     CDS             4577..5548
                     /locus_tag="LND56_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK6743563.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(5626..5862)
                     /locus_tag="LND56_001637"
     CDS             complement(5626..5862)
                     /locus_tag="LND56_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6743564.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(5831..5962)
                     /locus_tag="LND56_001638"
     CDS             complement(5831..5962)
                     /locus_tag="LND56_001638"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6743565.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(6038..6421)
                     /locus_tag="LND56_001639"
     CDS             complement(6038..6421)
                     /locus_tag="LND56_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK6743566.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(6454..7239)
                     /locus_tag="LND56_001640"
     CDS             complement(6454..7239)
                     /locus_tag="LND56_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBK6743567.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            7417..8517
                     /locus_tag="LND56_001641"
     CDS             7417..8517
                     /locus_tag="LND56_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6743568.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8507..9331
                     /locus_tag="LND56_001642"
     CDS             8507..9331
                     /locus_tag="LND56_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBK6743569.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            9328..10143
                     /locus_tag="LND56_001643"
     CDS             9328..10143
                     /locus_tag="LND56_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6743570.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            10140..10970
                     /locus_tag="LND56_001644"
     CDS             10140..10970
                     /locus_tag="LND56_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743571.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            10977..12008
                     /locus_tag="LND56_001645"
     CDS             10977..12008
                     /locus_tag="LND56_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743572.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(12115..13388)
                     /locus_tag="LND56_001646"
                     /pseudo
     CDS             complement(12115..13388)
                     /locus_tag="LND56_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            13615..13800
                     /locus_tag="LND56_001647"
     CDS             13615..13800
                     /locus_tag="LND56_001647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743573.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            13924..14640
                     /locus_tag="LND56_001648"
     CDS             13924..14640
                     /locus_tag="LND56_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK6743574.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(14542..15062)
                     /locus_tag="LND56_001649"
                     /pseudo
     CDS             complement(14542..15062)
                     /locus_tag="LND56_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15350..16003
                     /locus_tag="LND56_001650"
     CDS             15350..16003
                     /locus_tag="LND56_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743575.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         4900 a         2822 c         3462 g         4855 t
ORIGIN      
        1 taaagaatgt agcatatagt tatgcaaatt ctaaagaaaa agttttgtca ggagtaaatc
       61 aaaaatttga acttgggaag ttttatgcga tagtaggaaa gtcaggaaca ggaaaatcta
      121 cacttctttc cttacttgct ggacttgata aacctcaaac aggaaaaata ttgtttaaga
      181 atgaagatat acaaaataaa ggatatagta atcatagaaa aaataatata tctttagtat
      241 ttcaaaacta taatttaata gattatttat caccaattga aaatattaga ttagtaaata
      301 aatcagcaga tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa ataaaaagaa
      361 atgttatgaa attatctggt ggacagcaac aaagagtagc tattgctaga gcattggtat
      421 cagatgctcc aataatacta gctgatgagc ctactggtaa tctagacagt gttactgctg
      481 gagaaataat taatatatta aagacattag ctaaagatag aaataaatgt gtaatagttg
      541 taacacatag taaggaagta gcagattctg cggatatcat tttagaacta agtggtaaaa
      601 agttgaaaaa agtaaataaa atgaatttgg aggttgaata atgataaaaa atgcatttgc
      661 ttatgtaact agaaaaagtt taaaatcatt aattatcata ttagttattt tatctatgtc
      721 aactttaagt ctcattagtt tatctattaa agatgctacg gacagagctt caaaagaaac
      781 atttgctaat ataacaaata gtttttctat ggagataaat agacaagtaa atccaggaac
      841 acctagaggt gggggaaatg taaaaggtga agatattaaa aagatatctc aaacagatag
      901 tatagactcc tatgtaaaaa gaataaacag tgttgcagat ttagttgatt atgatattat
      961 tgaaactcaa gagactcttg cgaatcaatc acctgaaaga gcaaagaatt ttaaaagaac
     1021 agttatgtta actggggtta acgactcaac aaaagaaaca aaatttgtat cagaagcata
     1081 taaattagta gaaggaaagc atttagaaaa tgaagataaa aataaaatct taatgcataa
     1141 agatttagct gaaaaaaata accttaaagt tggagataag ataaaaataa aatctaattt
     1201 atttgatgct gataatgaga aaggtgcaga tgaaacagta gaagtagaaa ttaaaggtct
     1261 attcgatggg cataatagcg gtgtagtaag tgcagcacaa gaactatacg aaaatacatt
     1321 aatcactgat attcatacag ctgctaaagt ttatggtaat accgaagata cagctgtata
     1381 ccaagatgca acattctttg taaaaggtga taagaatctt gatagtgtaa taaaagatct
     1441 tgggaaatta gatataaatt ggagagaata taatttgatt aaaagttcat caaattaccc
     1501 tgcattacaa caatctatat caggtattta ctcaatttca aataaattat tttttggctc
     1561 attaatattt gcaggagttg tagtttcatt attattattc ttatggatga atgctagaaa
     1621 gaaagaaata gcagtattac tatcattagg tatatcaaaa ttagaaattt ttggtcaatt
     1681 cctaattgag atggtattta tatcaatccc cgcattcgtt ggttcctatt tcttagctca
     1741 atatacagct gacaagcttg ggaataatat attgaataag gttactggtg atatagctaa
     1801 acaaatagct aaacaatctg catctagcca attaggtggt ggggcagaag ctgaaggatt
     1861 taacaagaca ttatcaagtt tagatatcaa tgtattacct aaattcatga tttatgtagt
     1921 tatatttatg agtttagtac ttcttgtatc attgattatt tcttcattca atatattaag
     1981 aaggaatcca aaagaattat taattgacaa taattaaaat tttcggtgct ttttagcacc
     2041 aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca atcctaggcg ggtggttgct
     2101 tcacggtttt tatttttcta aaataagatc aagaggtgat tacacgatgg ctagaaagaa
     2161 aagaaatcct gatgctgaga agttagctga atccattttg aatacttatc aacccgaatc
     2221 tgtagaggat atgcaagatg ttttaaaaga tgtgtttggt cccttatttg aaaaaatgct
     2281 tcaaggagaa ttgaataacc atttagggta tgacgcccat tctaaagagc caaagaaaca
     2341 tgacaatcga cgaaacggtt atggcaataa gacgcttaaa acgagctttg gtaaagtaaa
     2401 cattgatgtt cccagagacc gggaggcttc ttttgaacct gagttgattc ctaatagaaa
     2461 acgagatgtc tccgacatcg cagactgtag acaaaagctg ttccaaactc gtaactcgag
     2521 cttggaacag ctttttatct acttcgtttc gaatttttga acaaaaaaat aaaggctcct
     2581 tgcctttcca tagccaggtg gctaatttct ttaaattcat tgcagcaaac actagcatcg
     2641 tatgcgtcgc gactttttcc aagcctcgat acttggtcca acgcatccca tgcttttctt
     2701 ttgcatccgc aaatattcgt tcaatcgttt gtttccgttt tttgtaaatt gatttattca
     2761 gatctgttag gcgaagatgt tccacttcat ccatcagatc tccccaaaca tgtctcgtga
     2821 tgactcgttg atgattttta cttatggtac attggtttag cagaggacag ttcacacatt
     2881 cttttggatt ggatttgtat tctcggtacc cttctcgagt aaccgttgaa aaagcgagca
     2941 tttgatcttg cggacaaata taacaatcaa aatactcgtc atagagaaac tcattttttg
     3001 ataatagctc ctttttccct ttgggccttg aataaggcaa acaaggtcga aggttcaaat
     3061 gagtcaaaaa gtgaacaaac ttaggtgtct tatacccagc atcagcgaca acacaattga
     3121 tttcaggaaa aagtctcttc gattgtttga cgagctgaaa ggcgacttga ctgtcatgaa
     3181 tatttctagg agtgatgatt gtgctaagaa taaatccatt gtcgtcgcat gaagtatgga
     3241 cagaataagc aaattgtttt tctcgttcgc ctttgacgta gtagccgctt tccggatcag
     3301 ctttacttat ttttctttct ttcagcttca cttccttttc taagtccaaa gtaaaagggc
     3361 gttttccagc ctctattcgt tgacgattga tctcatcttc taattcatcc tgataggctt
     3421 tcgcttcatc atgggtcatt tctttcgtaa acttatgttt attggcgtta gctttgatgt
     3481 gggtagaatc cagatacaaa ttatcctctg ttacaaaacc tgctttaact gcttgttcta
     3541 aaatataggc aaaaatatcc tcaaacaccg tcgtttctct aaatcggcga acataattct
     3601 ttccaaaagt tgagaaaggg gggattttct cctcgaagga gtagcctaaa aaccagcggt
     3661 aagcgacatt ggtatcgact tccttaatgg tttgtcgcat ggaacggata ccgaataaat
     3721 actgaataaa caccaatttg atcagaatca ctggatcgat acttgggcga cctaaatcgg
     3781 agtaagtggc ttcgacgatt ggataaataa attcaaaatc aagcgcggca tcaatctttc
     3841 gaacgagatg atcttgagga actagatcat caatcgtaag catcgcaaat tgatttctac
     3901 cattcgtatt gtttttagac atcatagcta tcactccttg cattcgtttt cataaatcta
     3961 ttataaaaga aaagactgta gacatcatcc ataaaatgga ctttgtctac agtctggata
     4021 acgtgatttt ttgctgtaat gacaaggttt tagctgtttc agataccatg tatccaaaaa
     4081 tagtttattg agaaaatagg agagtatttg atactaacta gtaggaaaaa atgagataaa
     4141 aagtaaatgg aatcatttaa ggtaatctga aataaaaaat atacagccct gtggtacttt
     4201 acctacaagg ctgtatatat tttttactct cagcgttttt gactccgtgt ctgactccgt
     4261 tttattgatt ttgaatgtta ttcaatgacc atttaacagg tcaaatttga tgtttttgat
     4321 tccgtatgac gccgtttgat atctagtgat atagtgattt gagtctacat cgaataaggg
     4381 aataaaccaa taaaaaatga tttagaatat taaaataaca aaaaagagga ctacctgtat
     4441 tagtcctctt ttttgaccag attttgacca gtctatttta catcctagta tatttatatg
     4501 agctaatttt atgattaaat ggtaagtttt tccttgtgtg ttttagttta aactagtgtg
     4561 taattcaaaa atctatatgg aaggaacagt attaagaata gaacagggtt ctctacatga
     4621 tggtgctggt ttacggaccg ttgtttattt aaagggctgc ccattacgct gtgcctggtg
     4681 ttcgattccc gaatcacaaa gcaagcagat tgaaaaagga tttggccaga ccatgaccgc
     4741 tgaagaggta atggatgaga ttgagaagga tgctgttttt tactttcact cggatggcgg
     4801 agtgaccatt agcggaggcg aggcactggt tcaggcggat tttgctaaag aaattttgca
     4861 aaaatcgaaa tacattggaa tcaataccgt actggaaacc agcttttgtg gtgcttataa
     4921 tgaaatccaa aaggtagctc cctatgtgga tacattgttt gtggatgtaa aaatgtttac
     4981 cagcaagctc cacaaacaat ggaccggctt agataaccaa caaattttga agaatattcg
     5041 tcgcttttta attgagtatc ctaactgcga agtgcgaatt cgagttcctg tggttccagg
     5101 tatcaatatg aacctgaccg agctgctgac gattgcttgt tttgtggcag atcttgatcg
     5161 gttcgtaccg ttagaattac taccgtacca ctgctatggt atgcacggtt accaagcatt
     5221 gggactggag tatccgttgg cagacacgcc agcgccttca cctaaagaaa tgtttgcatt
     5281 ggcagatcaa ctggctcgca cggttcctca tttgctggtc acagctttaa ccaaaacatt
     5341 tatttattta ttgaatccaa gctttatctt atggataaat gaaagacact cactagctgc
     5401 agccagctca agaatagagc ttttgctgaa gtcagtgagt gtctttttac gatgtttttt
     5461 cgaagttaat ttgtcgaatg aacactggct caatcggtac atcttcactg tttgtcggct
     5521 gagctgcgat cgctgctacg atttctaacc cttggtatac ctgtccaaaa acagtataac
     5581 gaccatccag ctccggtagt cctccaagct ccaagtatgc ttgctttaca ttagcaggtc
     5641 agcctgcgcc tttgtgtttt gcacaataaa aaactggttg ccgttggtgt gggggccgtg
     5701 gtcccctaag gccaaggcgc cattaaaatg ataaagtccg cgatgcactt catcagcgaa
     5761 aaagccctca tagctggaaa cagcttcatc accagaacca tccaaagcgc caccttgaat
     5821 aacaaattcc ttaatgacac gagccgaaag gtgtgttgtc ataaacgcct tgctcagcta
     5881 gggagcgcca gttggctaca gccaggggag cttggtcagg aaataagcgg agacgaattt
     5941 caccagcatc ggtgaccagc acacaaactt cttcctgctg cggtttttct aattgaaaga
     6001 gaggaaggtt tgcaactgaa acgccttgat cctgccatca aatatcgaaa atccgccact
     6061 cgaccttcgc ctgatcacca aggcgagcca ttcccaataa aacggtatag gcggtttcat
     6121 ccgcttgaaa ggtcaattgc agctgtcctc gtaaatcgtt gtagtcatct ggggcggcta
     6181 cttgataaaa aagatgaaac tgcgcatgca aatattccaa aggctgatct ttaaaggctg
     6241 caaaataagc ctgaactttt tcccgctgca agctgtcagc caccaaaaaa ttttccagtt
     6301 gttgttttgg aacggctgct aacaaggatc ggcaaaccgc ctcaattttc aacgcatccc
     6361 aatgtcgcgc aattaaataa tggatgggtg cctcttgatt gtagtcccac aggttgctca
     6421 tttttttcac cactttcttc tattatatag gaatcacaaa atcggcagcg gcgtcaggcg
     6481 gcgttctttt ccttgaatga ctagcaactt ttctacctga cagtctgcca acagctgact
     6541 cattttttta aagcccagct gataatcttc ggcaacatga gcatccgagc ctaaggtaaa
     6601 gagctttccg ccgacacttt gatacaaagg aacggcataa cggtaaaggt cttcgttgcc
     6661 gtatttcaag aagctcttgg catttaactc catcgccaat tctcgttcaa caaccttttt
     6721 gaaaatcgtt agcagctgag cctcaaaatg ctccgcgaat tcttccgctg tgaaggtgaa
     6781 gcggcgtaat ccataatcaa agtgagtcaa aatatctcct tctggaaaat ccgtcaagac
     6841 ctgcaacatc tgatcaaaat attccttggt agtttgaatg ggatctttgg ttaaaaccac
     6901 atcgtccata taatcaaagc ggccattttg atgaatactt accagcttta aatcataagg
     6961 atgctgagcg aggtaatcca cgatccgctc ttcttgtcct ggaacaacgc caatctcgat
     7021 acctcgcaga aatttggtgg aataggtttc ttttaaagag tccagtttgt ttatataggc
     7081 tggataatcc ggaatgtcat cttttccggt agatggattg tttaaatcaa agtgatccgt
     7141 agaaacgaaa aactccggct gataggctaa atagttttca aatttttcct ctgaatcaaa
     7201 ggaaaaatac gtgtggagat gctgatcata atactgcata gggagcctcc ttttttctac
     7261 tatcataccg caaaagcttt agaaggttaa cgggaattcg tcgaaattta caaaccatat
     7321 acaaccttta cattgttcat gaaacctttt caagaaactg attctctcat tacaaagctt
     7381 tgttagaatg gaattatcga aaggggagaa gaatgaatga aaatgaatga aaaaattcgt
     7441 aacatgacta tcggtttatt ttgtgcagca acagtaagta tcctgctgac aggctgcggc
     7501 tcaggtgaag caacgaagca atcggctgcg gcagacgact ctttagatac gattatagaa
     7561 aaagccaagg aagaaggaga gattgccagc ttgggaatgc cggatacttg ggcaaattgg
     7621 gtaggcactt ggaacgacct tgaaaaagaa tacggcttaa gtcacgtgga tacggatatg
     7681 tccagtgcag aagaattggc taagtttgaa tcagaaggaa aaaatggaac cgccgatatt
     7741 ggtgatgtcg gtatcagctt cggtccgttg gctgaaagca aagagttaac cttaccttat
     7801 aagaccaact attgggagga aattccggat tgggcaaagg atgatgatgg ggattggttg
     7861 ttaagctata cgggaaccat ttcctttatc accgataaag agaatgtgaa gcaagcaccg
     7921 actagctgga aggagctgca agagggtgac tatcaagtta gcgtggggga tgtgacgatt
     7981 gccaaccaag cacaatttgc tgtattggct gcggcgattg ccaacggagg tgacgaaaag
     8041 gatattcagc caggtctgaa ttactacaaa aaaatggcag aaaaaggccg tttgtccacc
     8101 gttgatgcca gcgtagcaaa tcttgaaaaa ggtgaaatcg atgtggcagt tgtttgggac
     8161 ttcaatggac taaattatcg tgaccaaatt gactccgagc gttttgaggt aactgtgcca
     8221 acagacggct ccatcattag cgggtataca accttaatca ataaaaatgc ccctcatccc
     8281 aatgcggcaa agctagcacg ggaatatatt ttatctgatg aagggcaaat caatttggca
     8341 aagggctatg cgcgacctat tcgcaaggta gagctgccta aagaagtagc tgaaaaatta
     8401 ctgccggagg atgcttatga agcggcggtg ccagttgaag acaatgaagc ttggaacaag
     8461 acctctgtag agcttccaga gctttggcag gcggaggtac tgacctatgc aaagtagact
     8521 tgacaaactg attgtagtta tactagacgg ctgtcgctac gacacagccg tcgcccagct
     8581 tgggttcatg aatcatttag ttgagcacca gcaagcaagc ttattgaagg taaaaagtga
     8641 gctgcccagc aattcacgtc cgttatacga agtactgatg accggcgttc cagcctatga
     8701 aaatggcatc tatagtaatt accatgctca gcgatccaaa gagtggtcgc tgtttgaatt
     8761 agttaaggaa gctggcggaa aaacaggagc cgccgcttac cactggtaca gcgagttgta
     8821 caatcaagcc ccctataatc gtttggttga tcgcatccag catcaggagg agcgactgat
     8881 tcagcatggc atcttttata gtgaggacac ttaccctgat tcgcatttat ttgcggatgc
     8941 ccattattta gtgaatcagt atcagccaga ctttttggtc gtacattcga tgaatattga
     9001 tgatgtcggt cacaaattta ccgccgattc tcatgaatat gcagcagcgg ttaatcaggc
     9061 ggacagatta ttggctattt accttcctga atggctgcaa aaaggttatc aagtcgtggt
     9121 gactgcggat cacgggatgg atgcgcacgg actgcatggc ggcagcttgg ctgcacatcg
     9181 tgaagttcct ttttatttga tttcaaataa acgccaaaag ctgaaggaac aaactgtacc
     9241 acagctgctg gcggctccat tgttttgctg gttattagga attgcaccgt cagagaaaat
     9301 gcaggacatt caagaattga tgaggggatg aaagagatga aaaaacgctt ggttctggtt
     9361 cctgcctcct ttatcttctg tgcattttta ctgctgccgc tgctttttat ggttctgtgc
     9421 agcttgcaca gtgatttaac taaccagtgg acattggcca attacagtga tattttcagc
     9481 aacaaatatt acacacaggc ctttgttaac agcgtactga ttgctgtcag ctccagtgtg
     9541 attgggttaa tcgggacgtt tattttatgt ttatgcttaa tgaatctatc gactaaattg
     9601 caggagaaaa tcacctttgt ttccaatttg gcagctaatt ttgctgggat tccgctggca
     9661 ttctctttta ttattttgtt ggggaatgta ggtgtgctga aaatgatttt gccatttcta
     9721 gcggacttca atttgtattc ttggtgggga ttgaccatca cgtataccta tttccaaatt
     9781 ccgttagggg tcttgttttt gtatcccagc atgaaggaag tgaaaaagga atggctggag
     9841 tccgcacaat tgctgggcgc ttcatcagcg tatgcttggt tcaaggtggc tttgcctttt
     9901 ttgcgaccat cgattgccag cacctttgtc atcttatttg ctaatggcat ggggacctat
     9961 gaaacagcgt atgccctgac cagcagtaat gtaaatcttt taacgattcg aattgcggct
    10021 ttagtgtcgg gagatgtgtt cgccaagcct aatttaggca gtgcgttggc agtcgcttta
    10081 ggtgtcctgc tggtaacggc aatgttgatt attcaaaaga aaggcaaggt ggtgcagacg
    10141 tgaaaaagtt aataaaaccg attttgttct gctttgtcct gtattacttg ctgccgatta
    10201 ttggaacgat tttgtatgcc agctccacta aatggtcaaa atcgctttta cctagtgatt
    10261 ttaccttgca atggttccaa cagctgctga cggacaggga atttattgct gcggtgggtc
    10321 gctcactttt attagcaggt gtggtcttgg tgacgattct gttgttgatg attcccacca
    10381 ttatttggat tcatttgtat ttccctcgtt tgaatcgttg gctggaaaag ctgctcctgc
    10441 tgccgtatgc tttaccagga gtgattttgg taacggcttt gcttcgaacg tatgctgaaa
    10501 ctggtatccc aatgtttgta gtgttggtag gtgcattgtt tattacggcg ttgccgattg
    10561 tctatcttag tcttaacaac cagatgcgct taattaatct gaaggaatta gtggatgcag
    10621 cagaaacctt gggcgccccg atgtctacga ttattatcca ggtgctgttt ccaaacatcc
    10681 gtatcggtgt gaccctagtt tctctgatga ttttttcttc ggtttttggg gagtacatgc
    10741 tgacgaacct gttgattggc ggtcgatttc agacggtacg catctatatg ctgcggcgga
    10801 tgaacgaaaa cggccatttg gctagcgctg tgatggtttt gtatttgctg tttatggtgg
    10861 gaattgcttt aactaccttt atgctgactt atcgacaaaa gcaacagctg tcccttaagc
    10921 agacgaggga agagaagcag tcgaaaaaga agcccttaaa ggagttgtga cggaaaatgt
    10981 ttttagaaat acatgatctc aatctgtttt ttgatgaaaa acagatttta gctattgatg
    11041 aaattcagat tgaaaagggt gagctggtta ctttactagg tcccagcggg tgcggaaagt
    11101 ctacgttgct gcgctgtatc acgggattgg aacaaccaaa gagcggtgag attgtgctgg
    11161 acaatgagaa tattgccgat aagcctacga aggatcgcaa tatcggcttt gtttttcaac
    11221 agtatgcatt gtttccaact atgactgttt ttgaaaatgt agcctttggt ttgaaggtaa
    11281 agaagctgag tcctgaggtg attcaggaaa aggtttttga gatgctatca ttggtggata
    11341 tgacggagca ggcagataaa aatgtccagt ttctttctgg cggtcaacga caacgggtgg
    11401 cactggctcg ctcattggtc actgaaccga aagtcctgct gctggatgag ccattaagtg
    11461 ctttggatgc ccgaattaga aaacagctgc agcgggattt gcgcgctatc cagcaatcgc
    11521 tagggatgac gatgattttt gtgactcatg accaagaaga ggcgatgcga atcagtgatc
    11581 ggatttttgt gatggaagct gggcgggtgg ctcaggtttc cacctcgaag gaattgtatc
    11641 agaatcctca gagccgattt gttgcggaat ttatcggcaa ctataatcgt ttcgaatggt
    11701 atgagctgaa tcagtatacc cacgagtttt ccgctaaggg caatcgctgc atttattatt
    11761 taagaccgga gctgattcaa tttgaaccgg ttcctcaggg ggttaagatt ccggttctat
    11821 ggaaggaatc atttatttta ggcaacattc aacggtatgt ttttcaaacg ctggagggca
    11881 aagaaatttt agtggatcgg ttgaacgatc gggaagagaa aattagcgat gccttataca
    11941 ttcagctgaa agatattcta gcaatccctg tggaaattga tactcagcat tttgcacaac
    12001 aaagctagtg tatagtcggc aaacatttct ggctctttgt caaataggac tgatagagtg
    12061 atataagaac atctaaggta gaattgattt tctgctttag atgttttttt atgtttaata
    12121 agtgatcaga tgattgatta agaaaattct ggttctcatc ctaacccgta agctattcgt
    12181 ttcaatgctt tgatgtgggt atgcagattt tctattttgc catttgagta aggcaactct
    12241 aaggctaaac gaatgccgga ttcatagttt ataaacgctt tttttatatg atgcagttct
    12301 cttggaaccg acttgggcat tgtttttaca agctgaaaga agatgtccgc ttctttgtca
    12361 taaaaagctt gaagaatttc ttgatagtaa gcataagcta ttcgtagttc atcagaagta
    12421 gaaagtagac gttctacgac ttcactttcg gttaaatagg cccaaccaaa attacgccat
    12481 tttgtccatt tatcataaac aagcgtgtcc tgtcgtttga tcagaagtcg agataaagcc
    12541 ttcacttgtc gatagtgttt ggcttgtatt ggatcgtttc ggtcgaagct tttcatgact
    12601 catagtcaaa tatccttaaa actgcgaatg aggtgcttga cgatgtgaaa gcgatccaca
    12661 acgagtttgg catgaggaaa gcactctttt atcagcaaag cataagaggc gttcatatct
    12721 gttacgagta tttttacgtt taaacgtgca cgtcgttcaa attgcataaa ataagaaatg
    12781 agatctttct tcttacgtga aggaagaata tccagtaatt tcccagtatc gccatccata
    12841 caagaaaaag aatagggacc gacttgattt ttcaatgaac gaaattcatc taccaatgaa
    12901 cagcgaggca accaatttat tttcacgttt gtcatgggga gcagagaagc gagtgttcgc
    12961 gaaacggttg accaagaaac gccgcaatgt ttagaaatcg tcgtcattgc ttgtttttca
    13021 gtcaattcct ccaatatttt ataatgtact tgtttggcga taaaacagtt tttttcaaca
    13081 atgtaggtat tcgccgtaaa ataggtgtga cagccacgac agaggtaccg ttgctttgcc
    13141 aaagcaagat aagtccgttg attcgcacac ttattgagaa ggaccagcga ttttttcttt
    13201 ccgttcttaa cgatcagccc atgttgattc acctgtttac actgaggaca gggctgaatc
    13261 gttttaatga gcgtggcaga aacaacgata ctgttgattg aatgtatttt tttcttctcg
    13321 acatgagtga tttgaatgtt tccatctttg attcctagta atagtttgat aagatgaaga
    13381 tcaggcatac agaacgctcc tttgattgtg ttgtggtgat tcaattctaa cggagcttct
    13441 gtatgttttg tttttttatg ctcaaaagga ctgatagaat gatttactat cagtcctaga
    13501 aattatagcg ccacatttct ttcacctttg acttttcaga cggcagtttt tatgctgata
    13561 ctgtagagag agcaggacac agcttttgct tgttttgggt aagggggaaa gaagatgacc
    13621 aaagcactta tttttgattt tgatgggacg atgattgaca gtgaatggat gttttacgag
    13681 agtattcatc actatttact gaagcctatt tactggagca gttcggctat aaaattcctt
    13741 ttgcagacta tcaacaaggt gttgggacgg cagcagaact gtccttttat gaagggataa
    13801 gtcaagcggt tgggagacca gtggatgctg aacattttga gcagcggatc agcagcactg
    13861 gaaaatcgga gtagtctcaa ttctgaactg cgggagctag actatttttt tgaagcacat
    13921 ccgatgatta aggaaaaaat tgattccttt gtgacgatcg atgatcttca ggtcggcaaa
    13981 ccagatccag aaggttattt gttgtgcttg aaacggctga gggttgaaca ggctgttgta
    14041 gttgaggata gtccagtcgg tgcccaagca gcaccgcata tgacagttga tgtacaagcg
    14101 ttggatgcag attttttcgc ctttagcggg cataaaatgt gcgggccgac aggaatcggt
    14161 gtattatatg gcaaacgtca attgttagaa caaatggagc cagtcgaatt cggcggtgaa
    14221 atgatagatt ttgtcaatct ttacgatagt tcatggaaag agctgccatg gaaatttgaa
    14281 gctggtactc cgaacattgc aggagcaatc gattacttga atgagattgg tatggagaat
    14341 attcatcgtt atgagcaaga actggttgcc tacgttttac ctaaattaca agcgatcggc
    14401 ggtatcacca tttacggacc gcaagatcct gaacatcata ctggcgtggt tgcttttaat
    14461 ttggatggca tccatccccg tgatgtggcg actgctctgg atatggaagg tgtcgctgta
    14521 cgttcaggac accattgtgc acaacggttc atcgacgatt tctgtttgac taattggtcg
    14581 tttaaacagt cgatgataat gccgtttgag cggctccaat gtcgtggaat ctttgagtaa
    14641 gagtttccaa tatcgcttga gccgacggta cttcttctga ttttcagatt acgtgattga
    14701 ttttcgtaca gttctatgag aatcgcctgc ttcagttcct ttgttagttg atgatgttca
    14761 tcaactaagt ccgttttcgc agaaaaggtc tgcccacaat catagcatag aaaacgggta
    14821 cgttttaatt ctaaaatagt caacttttct ttgactttca acatctgagt acgtgtcgta
    14881 taggaaccgt tgcggataat acgaatcgat ccacagtgtt cacaatggga aggtgagtca
    14941 tccaatctcc ctttaataaa ctgcgcttga acgcctttat attctttttc aatcagccat
    15001 tcttcatcaa aaaataaatg tttatctgtt aatccgagta attttctagt atgattatcc
    15061 atagagcgac tcctttggta gggtgatgtt ttggtcgact aaattttacc aaaagtgagt
    15121 ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg attttccaac
    15181 aacacaaaat attatagaag ccttttttct aaaggatttc aaggtgtctg tttgcattta
    15241 ttaaaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagagc
    15301 cataatatgc tgtataatat ctagtgtata ggcttaagtg gaggtatata tgaaaatatt
    15361 aactgttgaa gatgataata tgataagaga gggaataagt gaatatcttt cagaatttgg
    15421 atatactgtt attcaagcta aagatggaag agaagccttg tcaaaattta atagcgatat
    15481 aaatttggtt atcttagaca ttcagatacc ttttataaat ggtttagaag tgttgaaaga
    15541 aattagaaag aaaagcaatt taccaattct aatcttgact gcatttagtg atgaagaata
    15601 taaaattgat gcatttacta atttagtaga cggatatgta gaaaagccat tttcattgcc
    15661 ggtcttaaaa gctagaatag attctttaat taagaagaat tatggacatt tagagaagtt
    15721 tgaatataag gatctatcgg ttaattttaa tagctataca gctaaaataa atggtgaaga
    15781 aatagatgta aatgcaaaag aactagaagt attaaaatgt ttattggata ataatggaca
    15841 agtgttaaca agaatgcaaa ttattgatta tgtatggaaa gatagcgaag aaactcctta
    15901 tgatcgagta atagatgtat atataaaaga acttagaaaa aaattacaat tagactgtat
    15961 aactactata agaaatgtag gatataaatt ggagagaaaa tgaaaaaatt aaagatattt
    16021 ccaaaaatgt tcatacaaa
//
LOCUS       DAFCLG010000057        15932 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFCLG010000057 DAFCLG010000000
VERSION     DAFCLG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.057"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..1152)
                     /locus_tag="LND56_001651"
     CDS             complement(13..1152)
                     /locus_tag="LND56_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBK6743576.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="LND56_001652"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="LND56_001652"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK6743577.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /locus_tag="LND56_001653"
     CDS             complement(2965..3720)
                     /locus_tag="LND56_001653"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK6743578.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /locus_tag="LND56_001654"
     CDS             complement(3824..5014)
                     /locus_tag="LND56_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK6743579.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="LND56_001655"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="LND56_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6743580.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="LND56_001656"
     CDS             complement(6166..7257)
                     /locus_tag="LND56_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBK6743581.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="LND56_001657"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="LND56_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK6743582.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="LND56_001658"
     tRNA            8800..8871
                     /locus_tag="LND56_001658"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
     gene            complement(9063..9947)
                     /locus_tag="LND56_001659"
     CDS             complement(9063..9947)
                     /locus_tag="LND56_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6743583.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10034..10933)
                     /locus_tag="LND56_001660"
     CDS             complement(10034..10933)
                     /locus_tag="LND56_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBK6743584.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10950..11456)
                     /locus_tag="LND56_001661"
     CDS             complement(10950..11456)
                     /locus_tag="LND56_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBK6743585.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11645..12265)
                     /locus_tag="LND56_001662"
     CDS             complement(11645..12265)
                     /locus_tag="LND56_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBK6743586.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12341..12778)
                     /locus_tag="LND56_001663"
     CDS             complement(12341..12778)
                     /locus_tag="LND56_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBK6743587.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12941..13771
                     /locus_tag="LND56_001664"
     CDS             12941..13771
                     /locus_tag="LND56_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBK6743588.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13755..15122
                     /locus_tag="LND56_001665"
     CDS             13755..15122
                     /locus_tag="LND56_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6743589.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15141..>15932
                     /gene="yidA"
                     /locus_tag="LND56_001666"
     CDS             15141..>15932
                     /gene="yidA"
                     /locus_tag="LND56_001666"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK6743590.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAI"
BASE COUNT         4308 a         3333 c         2938 g         5353 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgtccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt tcgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtccg acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aaatctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 agaaaattgg tggacagttt tttgttgtac ctgtatagat attgttaagc ctttgcaagt
     9001 aaggtagtta tgtgttcaac tacttattaa aatgtgttat ttattaaaga tacttgtctc
     9061 acttactgaa ctggccgatg atcgtttctg ccataatagt tctcgacagc cgttggatgc
     9121 cctaataaat ccccaactgt cgtgatgcga tacccttgct ctttcagata atcgatcact
     9181 tgaggaacgg cacgaatcgt ttcaggatag atatcatgga ataaaataat tgccccatcg
     9241 tatactgttg cttggatctt ctgcaaaatc aaatcaggat ttcttgtctt ccaatcttct
     9301 gaatcaacag accattcgat gatcggtcgc tggatgatcg ttgctcctac attcgtgaca
     9361 gaaccatagg gcggacgaac aaaagtgggg gttttgccag tgactttttt gatctcagtg
     9421 tctgctttcg tcacttcctc cgtcatcgac tgggcagaaa gagtggccag attctgatga
     9481 tcgtatgtgt aattgccaat ctcgtgccct gcttcagctg tctgcttcac gatttcagga
     9541 tgctgcgccg tattttctcc taagacgaaa aaagtggcat gcacgttttt ttcttttaag
     9601 attcgcagaa gctgcggtgt tgtggtcgct cctggtccat catcaaagct gattgcgacg
     9661 cttttgacag ccggatctcg gctcgttttt gtactgtctt gtgttgattg tgatgactga
     9721 ctttgtacct tttccgctga ttcttgaatg gcttctttga cgttagctgt ttcagttgat
     9781 tggcttttaa tggttgattg ttctgattgt ttgaaagatt gtgacgaatg ctccgaagag
     9841 tgtgtttgtc cttttacttc ggtgatcaag gaggccaccg aagtaatttc gaaaaacaaa
     9901 agcacggata aagcaataat actgataact aaataatttc ttttcactat atctcttctt
     9961 tctctttttc tcttcttatt atttacttgt acttacttga aaattgaaac tgctagtcgc
    10021 aactacttct cgtctaatga tccaaataat cttccgatgc caaaatcgtt tttgtcacgg
    10081 acttaggaat gacaaattct ggtgtaccca tataaccagg agcaatttcg tattgagaaa
    10141 aagtcaaaac taattgatgc tctttgttca aataaaaact tcgtgtaggc gtaacaagtg
    10201 ttggtttttc tatcgtccct tcttgaatat cttgctcagt ccaatagtac ttagatggat
    10261 cttccttgac ttgttcggcc atctgccgtt tgacttcttt tgtcaaaaca gatagatagg
    10321 tgtcatcttt gaataaaagt ggaagtgaga gctggacact gtttttcttg tcgattgtgt
    10381 cgtatctttt ttcaacatgg ctatctgctg ctgtttgggt gatctgcctt tcaatcacga
    10441 gaaaccggtt atcgtctacg attttctgat aatctcccaa aacttggtaa tgatccgttt
    10501 caaaatttcc atattctttt tcagcttttt caaaatctgc ttgtccttcg cgaaagtatt
    10561 tttgatttaa tccatgaatt gttttatttt ctttctcatt ttgtgtattg atcttcggta
    10621 cctgaatatc gattttttca gatcgatcag taaagcgttc gcctaagatc aattcaatga
    10681 ctgatcctaa gatgggtaaa tcttctgcaa agcttcgaac ttgactatta aaaagaaggc
    10741 tacttgttgt aatcattacg atagccaaag atactccgaa cccttggaga cgtctctttc
    10801 tccgctttgc ttttataaat gattgctctt cgataataaa acgctggcgg atctcctctt
    10861 taagcccttt gggaattgct tgttctttat aagttttctt tagttttttt aaattttctt
    10921 tttgggaata catggcactc ttctctcctt cattctagga tttgacgtaa agcattcaat
    10981 gagcggtaca ttttcgtttt aactgtgttt tcattcatat ccaagatagt cgcaatttct
    11041 ttcaaacgaa atccttcaaa aaagtagagg atcagaatct ctttttccgg actgtctatt
    11101 ttatctaaaa tcatatggag ctctgcaacg gaagtttcta aaggtaactg ttgggggata
    11161 tcttgaattt caaataagtc cacaggcgtc cttttttgtc tgcgccaact atctaatgcc
    11221 gtgcggatca atatccgata aaaccatgca ctaaaatgct cgatctcttt ttctttttca
    11281 aaatagttca gggctttatg aaaactatct tgcatcacat ctaaggcctc ttctttatca
    11341 tgtacgtaat tcatcgtgat acggaacatt ttttgataat cagtttcgat gagtccattc
    11401 agcaggtgac ggtatttttc gatctgccgt tttttcttat atccagtaaa aatcattctc
    11461 cactcctttc tgcctcactt aatatgacgt acaaggtgcg ctaaaagttt caattttttt
    11521 attttttatg tatttacttg ggatttttat atacaaaaaa gaaggtgtga caaaaatcgt
    11581 gtcacacctc cttattctga taggctgatc gtctctgtca caacctctcg taagaaaata
    11641 atgtttattc tttttcagag tcagaaaatt cttcctcttc ataatcttca tcatcatcgt
    11701 cttcgtctat gatacttaaa tcttcttcga tatttcctgg caagtcttct tcatcatctg
    11761 tcgggtcatc tgctccgatt tcttgcaagt cagaattata agcagcgatt tcttcatctt
    11821 catcatcatc gtcatcaaac agatcatcgt catcttcttc tgtcagatct gtatcttctg
    11881 ggtcatcgtc attgtaatcg atcgcgtctt catcatcatt gataaaggcg ttgactttct
    11941 tgcgtttacg gcgacgtgga gtatcttcat cctcttcgtc cattccatgg tttacttctt
    12001 catcgatcga atcgatagca taccatgaac gcaagcccca acgattatcc cctaatgaga
    12061 taaagctgcc gtcaatattc aagtcagtat aaaattgtgc gagtgaatca cgaatttcac
    12121 tgtctgattt cccaagatag ttttggatct ggttagctaa atcagaaaaa tccatgacat
    12181 cgccacgttg ttctaagatc gcgtgtgcta cttcgatcat tgataattcg cttttgtttg
    12241 caccttcaaa tacactaatt tccaatcagg acacgtcctt tcacagtcta ctccttatca
    12301 tacaaaatca gacacaaaaa atcaaacttt tcttttatat ttacgctgta aaatccaagg
    12361 tcaaataata ttcgccgatt ttttccggtc ccatgaacag atcataatcg attcgaacct
    12421 tccctcctgt gggacggtct ttcaaactaa catgcagttc tttcgtaaaa gtagcaatct
    12481 ggatcatacc gtatggtgtt cgataggatg tttctaaccg ttctttatag ccaaatttca
    12541 aacgcatccg catttctcct gcacggatca gctgtacatg accatctgga actaatttga
    12601 tcgttacagg aatctcatcg cccgtctcct cctgaatctc tttataacgt atgtacagcg
    12661 tatcgccaat tgtcaccatt tgccctttca aatcaaaata aaaatcttgc acttcccctt
    12721 gctgttttac ttttgtacgc aatttgattg aaatcggtgt tccttgtgat aattccatta
    12781 ttcattctcc tttacttctc atagctacat tgttcctagt ttcttttgtt ttgtcaacca
    12841 acttgtccat ttttacagaa aacctttttt ccaaaacaac tctttgattt tagagtataa
    12901 tattgtagaa aatatgaatt tatacaaagg agccggttga atgaaccctg cgattcgact
    12961 agatcagcac atttatcaaa aaaacgactt ttcccctttt gcattaacag atatattaac
    13021 ggaatattct aaagaacacc agcaaatctt tttacatttc tggcaatatg actgcacagt
    13081 catcttaggt atgaaggatg ttcgaactcc ttttctaagt gacgggatcc aaacactgaa
    13141 agatgcaggt tacgctccag tgatcagaaa ttcaggtgga ttagggatcg tatcagatga
    13201 aggaattttg aatatttctt tgattttccc ccaaagtgac acaaaaaaaa taaccataga
    13261 tgacgcttac gagcaaatgc tcctattgac taaatacgct tttccaaagg caccgataca
    13321 agcattcgaa atcacccatt cttattgtcc tggaacttat gatttaagca tccatggcaa
    13381 aaaatttgcc ggtatcgctc agcgtcggat caaaaatggg atttctgtta tgatgtactt
    13441 aagtgtcaac ggtaatcagg aaaaacgcgg gcaagtcatg aagcgatttt atcaatctgc
    13501 tttaaaagaa gactttggta caaatggcta tcctgcagtg gatcctgcta caatggcaac
    13561 acttgaagaa ctgcttgacc aacccctttt tgtaaaagaa gtcaaagaag catttgcaaa
    13621 agcttttgaa aagctgtatt ctgcaagtga tttacaggat tctaccgact ggctaaaaaa
    13681 agaaatccag ctggagcaat ggaacaacca aatccagaga atggaacaac gtaataacat
    13741 aaaggagcta cattatgaca accccttata aatatcaaat gctgcctatg gaaaaagttt
    13801 ttcgtgatcc agtccataac tatatccatg tccagcatca agtaatctta gatttgatca
    13861 attcgaaaga agtccaacgt ctacgacgca ttaaacaatt aggcacttct tctttcactt
    13921 ttcacggagc agaacatagc cgttttactc attcattagg cgtttatgag atttcaagac
    13981 gaatctgcga tatcttttct cgaaattttt caaaagaaaa aataggcaac ggaggctggg
    14041 atgatcagga acggctggtg actttatgtg cggcactgtt gcatgatgtc ggtcatggtc
    14101 cttactctca tacatttgaa catatcttcc atacgaatca tgaagccatc actgtagaga
    14161 tcatcacttc tccagaaaca gaagtgtatc agattcttaa ccgagtagaa gaaggttttc
    14221 ctgaaaaggt agctagtgtg atccaaaaaa cttaccccaa cccacaagtt gtccaaatga
    14281 tttctagtca gatcgatgca gatcgaatgg actatttgct tcgggatgct tatttcactg
    14341 gaacagaata tggaacattt gatcttaccc gaatattacg tgtgattcgt ccttatgaag
    14401 gcgggatcgc tttttccatg agtggcatgc atgcagtgga agattatatc gttagccgat
    14461 atcaaatgta tgtgcaagtg tacttccatc ctgtttcccg tggtatggaa gtaattttgg
    14521 agcatctgct tcatcgcgca cacgagttgt atcaagaaga tgccagcagt tttgaactcc
    14581 attctcaatt acttctgcca tttcttgacg gaagctttac tttagaagaa tacctcaaac
    14641 ttgatgatgg ggtcttgggc acttacttta ctcaatggtc tgaatctgct gatcctattt
    14701 taaatgattt agctaaacgt tttttgaatc gtaaaccact gaaatctgct actttttctg
    14761 gcaaccggga ttcaaaatta gtccaagaac tcactttgtt agtagaaaag gtcggatata
    14821 atcctgttta ttacactgct gtgaattcta gctacgatct accttatgat ttttatcgcc
    14881 cagaacaagg cagacatcgc acacaaatcg agattttgcg aaatgacgga actttgatag
    14941 aattatcaca ggttagtcag cttgttgctg cattagcggg acaagaacaa ggagatgagc
    15001 gatttttctt tccaaaagaa atggttgatc cgtccttgcg tgatcattat gatttatttg
    15061 acgaaactta tcaagaattt gccagccata tccgtaatgg cgcattgatt gaaataaatt
    15121 aaaatatatt ggaggaaatt atgtctatca aactagtcgc tatcgacatc gatggtactt
    15181 tattaaatga gaaaagagaa gtaacaaaag aagtaaaaga agcgattgcc gctgctgttg
    15241 caaaaggtgt ttcgatcgtt ttatgcactg gccgtccctt accaggtgtc caggaacagt
    15301 taaacgagtt gaaccttttt caagataatg attacgttat tacttataac ggggcactcg
    15361 tacaacaaac gaaatcaggt aaaatcatcg cccgtcacgg actgacacat gaagatttcc
    15421 tagagatcga agtgatggca cgccgtgtcg gcagtcatct ccattcgatc gatgacaaag
    15481 cgatctacac gcctaatcga gatatcagcg cttatacgat ccacgaagca agtttagtca
    15541 agatgccgct aaaatatcga acacctgaag aaatgacacc agacatgaac atcgtcaaaa
    15601 tgatgatgat cgacgaacca gaaattttag atgcagcaat tgcgcgttta ccgcaaactt
    15661 tccgtgataa atacacaacg gtcaaaagtg cgccctatta cttcgaagta ctaaataaag
    15721 aagcaagtaa aggtgcggca gttgcgaatc tcgcacagca ccttggtatt gatcaagatg
    15781 aaatcatggc tatcggagat aacgagaacg acttatccat gattgaatat gctggtctag
    15841 gagtagcaat gggaaatgcc gtttctcttg tcaaagaagc agcaaatgtc atcacttcat
    15901 caaacgacga gcacggtgta gctgaagcaa tc
//
LOCUS       DAFCLG010000058        15926 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFCLG010000058 DAFCLG010000000
VERSION     DAFCLG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.058"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..3654
                     /locus_tag="LND56_001667"
     CDS             127..3654
                     /locus_tag="LND56_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBK6743591.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="LND56_001668"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="LND56_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBK6743592.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="LND56_001669"
     CDS             7442..7783
                     /locus_tag="LND56_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743593.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="LND56_001670"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="LND56_001670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK6743594.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="LND56_001671"
     CDS             9140..11560
                     /locus_tag="LND56_001671"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK6743595.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LND56_001672"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LND56_001672"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK6743596.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="LND56_001673"
     CDS             12994..13731
                     /locus_tag="LND56_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6743597.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="LND56_001674"
     CDS             13745..14560
                     /locus_tag="LND56_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6743598.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="LND56_001675"
     CDS             14573..15220
                     /locus_tag="LND56_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6743599.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSTLAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="LND56_001676"
     CDS             15236..15892
                     /locus_tag="LND56_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6743600.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
BASE COUNT         5342 a         2745 c         3375 g         4464 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagca ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaa
//
LOCUS       DAFCLG010000059        15825 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFCLG010000059 DAFCLG010000000
VERSION     DAFCLG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.059"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(266..466)
                     /locus_tag="LND56_001677"
     CDS             complement(266..466)
                     /locus_tag="LND56_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6743601.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="LND56_001678"
     CDS             complement(771..2003)
                     /locus_tag="LND56_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK6743602.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2260..2829
                     /locus_tag="LND56_001679"
     CDS             2260..2829
                     /locus_tag="LND56_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6743603.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3007..3447)
                     /locus_tag="LND56_001680"
     CDS             complement(3007..3447)
                     /locus_tag="LND56_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK6743604.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3605..4369
                     /gene="map"
                     /locus_tag="LND56_001681"
     CDS             3605..4369
                     /gene="map"
                     /locus_tag="LND56_001681"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK6743605.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4401..5324
                     /locus_tag="LND56_001682"
     CDS             4401..5324
                     /locus_tag="LND56_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBK6743606.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5400..6539
                     /locus_tag="LND56_001683"
     CDS             5400..6539
                     /locus_tag="LND56_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBK6743607.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6532..7332
                     /locus_tag="LND56_001684"
     CDS             6532..7332
                     /locus_tag="LND56_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6743608.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7332..8159
                     /locus_tag="LND56_001685"
     CDS             7332..8159
                     /locus_tag="LND56_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6743609.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8137..8871
                     /locus_tag="LND56_001686"
     CDS             8137..8871
                     /locus_tag="LND56_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6743610.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8971..9837
                     /gene="rfbA"
                     /locus_tag="LND56_001687"
     CDS             8971..9837
                     /gene="rfbA"
                     /locus_tag="LND56_001687"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK6743611.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9851..10423
                     /gene="rfbC"
                     /locus_tag="LND56_001688"
     CDS             9851..10423
                     /gene="rfbC"
                     /locus_tag="LND56_001688"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBK6743612.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10445..11473
                     /gene="rfbB"
                     /locus_tag="LND56_001689"
     CDS             10445..11473
                     /gene="rfbB"
                     /locus_tag="LND56_001689"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK6743613.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11571..12422
                     /gene="rfbD"
                     /locus_tag="LND56_001690"
     CDS             11571..12422
                     /gene="rfbD"
                     /locus_tag="LND56_001690"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBK6743614.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12456..14489
                     /locus_tag="LND56_001691"
     CDS             12456..14489
                     /locus_tag="LND56_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743615.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14533..15813
                     /locus_tag="LND56_001692"
     CDS             14533..15813
                     /locus_tag="LND56_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK6743616.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4862 a         2732 c         3280 g         4951 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccctcaataa acagtcgaag agaaaatagg caaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caacagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatccg acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aatgactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg cagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccag ccgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac actgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc ctttaaataa aaaaaagagg
     2101 ttattttttt aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa
     2161 gaaaatcaaa aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg
     2221 gtggaagcgt tataaaaaaa ttaaggagat taaaaaaata tggcaagaaa aaagacgatt
     2281 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     2341 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     2401 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     2461 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     2521 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     2581 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     2641 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     2701 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     2761 attgaaaaga tgatacaaga cagtatagat gctatcttga aattcaaaga accaatagaa
     2821 atcgattgaa tatactcaaa agttccacaa atcagtagat aacaagagat gtgtcaaaat
     2881 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     2941 gaatgcaatg aataaaagag ccgtacagat tgaataaatc tggatggctc ttttattgca
     3001 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     3061 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     3121 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     3181 tccgcagaca ccgtaaattt ttcccgatag atccaattct tgcaagtctt cataaaagtc
     3241 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     3301 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     3361 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     3421 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     3481 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
     3541 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     3601 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     3661 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     3721 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     3781 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     3841 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     3901 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     3961 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     4021 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     4081 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     4141 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     4201 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     4261 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     4321 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     4381 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     4441 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     4501 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     4561 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     4621 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     4681 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     4741 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     4801 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     4861 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     4921 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     4981 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     5041 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     5101 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     5161 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     5221 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     5281 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     5341 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     5401 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     5461 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     5521 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     5581 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     5641 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     5701 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     5761 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     5821 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     5881 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     5941 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     6001 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     6061 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     6121 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     6181 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     6241 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     6301 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     6361 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     6421 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     6481 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     6541 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     6601 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     6661 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     6721 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     6781 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     6841 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     6901 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     6961 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     7021 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     7081 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     7141 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     7201 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     7261 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     7321 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     7381 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     7441 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     7501 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     7561 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     7621 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     7681 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     7741 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     7801 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     7861 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     7921 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     7981 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     8041 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     8101 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     8161 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     8221 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     8281 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     8341 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
     8401 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
     8461 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
     8521 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
     8581 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
     8641 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
     8701 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
     8761 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
     8821 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
     8881 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
     8941 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
     9001 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
     9061 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
     9121 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
     9181 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
     9241 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
     9301 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
     9361 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
     9421 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
     9481 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
     9541 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
     9601 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
     9661 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
     9721 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
     9781 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
     9841 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
     9901 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
     9961 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    10021 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    10081 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    10141 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    10201 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    10261 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    10321 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    10381 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    10441 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    10501 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    10561 aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    10621 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    10681 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    10741 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    10801 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    10861 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    10921 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    10981 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    11041 tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    11101 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    11161 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    11221 cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa
    11281 cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt
    11341 aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    11401 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    11461 aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag
    11521 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat
    11581 taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc
    11641 tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg
    11701 cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag
    11761 ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa
    11821 atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg
    11881 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    11941 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    12001 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    12061 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    12121 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    12181 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa
    12241 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    12301 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca
    12361 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    12421 aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    12481 gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc
    12541 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    12601 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    12661 ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac
    12721 ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa
    12781 acggacaaaa catgatcatt gcgtataatt ctcctgcaaa ggatattacg gttatcggaa
    12841 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    12901 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    12961 gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    13021 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt
    13081 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    13141 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    13201 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    13261 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    13321 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    13381 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    13441 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    13501 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    13561 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    13621 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    13681 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    13741 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    13801 catggtctta tttctatgcg attacgcatt cgaaaataga taattatttt tcggattttg
    13861 aaaccatcgc agttgctgtg ttgggaacag tgattttagc atttgcatta tttggctgga
    13921 gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc
    13981 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    14041 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    14101 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    14161 aggaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    14221 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    14281 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    14341 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    14401 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    14461 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    14521 gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    14581 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc agggttaggt
    14641 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    14701 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    14761 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    14821 ggagatttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
    14881 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    14941 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    15001 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    15061 ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca
    15121 gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc
    15181 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    15241 gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    15301 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
    15361 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccaa
    15421 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    15481 ttgataggat ttgggattgc tactttgctg cttatccttt ttattggtcg cttaacgttg
    15541 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    15601 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    15661 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    15721 gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc
    15781 gctcattctt ccaggatcca acgctatcat tgataggcac aaaat
//
LOCUS       DAFCLG010000060        15688 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFCLG010000060 DAFCLG010000000
VERSION     DAFCLG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.060"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..2046
                     /locus_tag="LND56_001693"
     CDS             28..2046
                     /locus_tag="LND56_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6743617.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2116..2322
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2473..4887
                     /locus_tag="LND56_001694"
     CDS             2473..4887
                     /locus_tag="LND56_001694"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK6743618.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5235..6788
                     /locus_tag="LND56_001695"
     CDS             5235..6788
                     /locus_tag="LND56_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6743619.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7169..8953)
                     /locus_tag="LND56_001696"
     CDS             complement(7169..8953)
                     /locus_tag="LND56_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743620.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8968..10689)
                     /locus_tag="LND56_001697"
     CDS             complement(8968..10689)
                     /locus_tag="LND56_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743621.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10789..11550
                     /locus_tag="LND56_001698"
     CDS             10789..11550
                     /locus_tag="LND56_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6743622.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            12517..12785
                     /locus_tag="LND56_001699"
                     /pseudo
     CDS             12517..12785
                     /locus_tag="LND56_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            13457..>15688
                     /locus_tag="LND56_001700"
     CDS             13457..>15688
                     /locus_tag="LND56_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743623.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         5188 a         2739 c         3227 g         4534 t
ORIGIN      
        1 agataaattg aagttggagg atagaagatg gaaaacattg caagaaaaga acggagacgt
       61 ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt gggaactgcg
      121 atggtaggct gttctgtcgc tgccccatta gtacagccgg ttcctgttca agcggatgaa
      181 acacctgttc agttcggcgc aagaatcaat acagcggcat ttatcacaga aattgctact
      241 tatgctcaac caatcgctca agccaacgat ctatatgcat cagtgatgat cgcacaagca
      301 gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag caccttatta taatcttttt
      361 gggataaaag gcagctatca aggacaaacc gtctacatgg atacgcttga atttttaaat
      421 aatcaatggg taaataaaaa ggagcctttc cgacaatatc cttcttttgc tgaatctttc
      481 aatgacaatg cctatgtact tagaaatact tcatttggca acggttatta ctatgccggt
      541 acttggaaga gcaacacgaa atcctataca gatgcgacag cttgtttgac agggcgttat
      601 gcgactgatc ctggttacgc aggcaaatta aataatatca tcgcaactta cggattgacc
      661 caatatgata caccagctag cggaaacgca ggcggtggag cgactacagg tggcaataat
      721 actggaagtg caggtagtac tggcggttca agcaattctg gtaatacaaa ctcaggttca
      781 gcaacttcga ccacttatac tgtccaatct ggtgattctg tttggggaat ctcaaataaa
      841 ttcggtatca caatggatca attgatccaa tggaacaaca tccaaaacaa tttcatttat
      901 ccaggacaaa aactgacaat caaaggtagc caggaaaatg gctcttcgac gaataattcc
      961 ggtaacaata ccaactcttc aggaaatgct ggtacatcaa acggaggaca aacaactggt
     1021 gcgaaataca ctgttcaatc tggtgattct gtttggaaga tctcaaatga tcacggaatt
     1081 acgatggctc aattgattga atggaacaat atcaagaaca atttcgttta tccaggacaa
     1141 cagctaatcg tatcaaatgg cggttcttct aatgctggag caaatactgg ctcaaatact
     1201 gggaatacaa gtggctctag ttcgtccagc tcaagttctt ctaatacagc ttcaggaacg
     1261 aaatatacag ttaaagccgg agactctgtt tggagcgtct caaataaata cggaattacg
     1321 atggctcagc taatccaatg gaacaatatc caaaataact ttatctaccc aggacaacaa
     1381 ctgactgttt caaacagtgg atcaacaaac agcacgtcag gtaatacagg ttcgtcaacc
     1441 aatacaaata acagcagttc agttaatact ggcagcagtt cgtctcaagg cacttatacc
     1501 gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac gatggatcag
     1561 ttaatcgaat ggaataatat caaaaacaac ttcatttatc caggacagca attgatcgtt
     1621 aaaggtggaa cttcggcaaa tacaaataca ggttcaacta cttctgcagc gaaaccaaat
     1681 acaccgaata cgtctgctac gacaagtaca tcatccactg gtaatacgat gtataccgtg
     1741 aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc atatcacgat ggatcagtta
     1801 atcgaatgga ataatatcaa aaataatttc atctatccag gacagcaagt gattgtgaag
     1861 aaaggttcat ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac ctatactgtg
     1921 aaatctggag aatctgtttg gagtgttgct gacagtcatg gaattacaat ggctcaacta
     1981 atcgaatgga acaatattaa gaacaacttt atttatccag gtcaaacgtt gattgtagct
     2041 aaataaaata gctgttcgaa gaaataacga ttgaattcca caaaaatatt tgttactata
     2101 aggctactta aatatgacaa cacttgaaaa ggaagtagta aaaagactga agtttcagag
     2161 agcgcatggc tggtgagaat gtgcacggat gcttttgaac tcgcctttga gttgcccttg
     2221 ctgaataccc gagtaagcaa gcggcggtga cggccgttac aacgttcgag gccttttcac
     2281 taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat aaaaagagca atctttttat
     2341 aggatttttt tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa ataaaaataa
     2401 acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt caatagaaca
     2461 ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca aaaatactgg
     2521 gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt ttacgcactg
     2581 gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga aggttatacg
     2641 gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct gcatccaatg
     2701 gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg aaacgatcca
     2761 gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc tttaggattt
     2821 agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa atggacacaa
     2881 tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt agcggtaaac
     2941 tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg gaaaagtgaa
     3001 cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa aatcactgct
     3061 tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag cattaaagaa
     3121 atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa agtggctggt
     3181 acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg tgcaacttac
     3241 tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga acaaaaagct
     3301 gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag aacggatctt
     3361 gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt taatggcaag
     3421 gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg agccatcatg
     3481 gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg actagaaatt
     3541 cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga tggtacgcac
     3601 atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa aatgaatgaa
     3661 tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg tgactggcta
     3721 ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga ggacggaaca
     3781 gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa aaacatcaag
     3841 ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa tgtcgttgat
     3901 cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg ggcaggaagc
     3961 tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc agattatgaa
     4021 aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca tgcggtactt
     4081 catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt tgttcctaca
     4141 aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa caacgaaaaa
     4201 atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac ttacggtgca
     4261 gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat tgcttggagc
     4321 gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt gatcgttgat
     4381 gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct tgataaagtg
     4441 tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt caatacagca
     4501 atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct gccttatgaa
     4561 tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg tgaagaacta
     4621 tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac atacgatgaa
     4681 gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa agttcgtgcg
     4741 aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc attagccaat
     4801 gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc ggtaccaaac
     4861 aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact gatgagttgt
     4921 gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt gttatttcaa
     4981 attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca cgttcaatgt
     5041 ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt tgtctattaa
     5101 tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca aattcataaa
     5161 ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat gaagtatgag
     5221 aaaaggagag acaaatggat caagaactat ttattatcga agtattatca gctaaacaaa
     5281 aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt ttaagcttag
     5341 tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc catatgagtg
     5401 cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac ttttcgtggg
     5461 gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt tggctccgtt
     5521 accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa cagtttttac
     5581 ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact tcgacgagtc
     5641 aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt gatgatttac
     5701 ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat atttttttac
     5761 tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac ctgacaacta
     5821 agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca atctattatg
     5881 ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac atgacaatga
     5941 actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct gatcctaagc
     6001 aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca gagacgcact
     6061 ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt gattcgcaat
     6121 ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt tattggatgg
     6181 acaacacata tgatatttcc gccgatttct catggtttat ctattcttct ggggatgatg
     6241 tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt atcacccaat
     6301 cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt ctatttatct
     6361 gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact atggatggcg
     6421 gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa aatgtcagct
     6481 tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat acaaacaacg
     6541 gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag acagggggga
     6601 tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg ttagagacaa
     6661 aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc tgcctcttcc
     6721 gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa gtagaacaag
     6781 gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa gaaacaaatt
     6841 caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag ttgacaagag
     6901 caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg tcttattgcc
     6961 gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa gtcttttgtc
     7021 actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta ttattctttg
     7081 tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac taatcataca
     7141 tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta ttataaagac
     7201 tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg acgatatcgc
     7261 cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg tgtgcaatca
     7321 caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat gcttctgttc
     7381 gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc gctagaatga
     7441 ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt tcgctagaaa
     7501 tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt gcgatttcag
     7561 ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata ttatcagcta
     7621 ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga cgtagttctc
     7681 cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta ttcagttcat
     7741 aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga ccaacgatcg
     7801 ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt ttatctgatg
     7861 tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct aaaagtcttg
     7921 catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc ctatcgattg
     7981 ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt ggttcagaaa
     8041 tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca cctgatagaa
     8101 caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg aaacgaatgg
     8161 ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat tgctttttat
     8221 ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg accactagat
     8281 taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac tgattctgtt
     8341 cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg ccaacgatca
     8401 gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga ccagcagcag
     8461 ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact tgagaaagtt
     8521 gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg tcaaaaaacg
     8581 acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat gtgatctttt
     8641 cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt aagactgaac
     8701 agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg aaagaaagct
     8761 gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt cccatgatca
     8821 agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat ccgtaaaaat
     8881 atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg ttcaacgttt
     8941 ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa actcctgata
     9001 agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt ttccttcatc
     9061 taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa caatcacgag
     9121 agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg ttttatagtc
     9181 taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca acgctcgagc
     9241 aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag caacaaagct
     9301 gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct tgatcgcttt
     9361 agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta aaattgtccc
     9421 gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct gttcttgagt
     9481 catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt cactaagtct
     9541 caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga tcgctgttgt
     9601 ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga tttcagtata
     9661 tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg gttcagctac
     9721 tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt ctatccgtcg
     9781 taatgaggca aacgatttag ggataatgac caataccata gctgcgatca tcaagtaagc
     9841 aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta atgctccttc
     9901 tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca tgagcaaaaa
     9961 agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat tagcatagtc
    10021 agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg cacggatcat
    10081 attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt ttggctggat
    10141 actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata cataaaaaac
    10201 aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca tgagaatggt
    10261 gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa tcatttgctg
    10321 gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa aacgactgat
    10381 atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt ggaatccaaa
    10441 cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg ttcctagaaa
    10501 cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc cacttgctat
    10561 acctgtgtcg atcagctttg ctactaattt tggtacacct aatgtaccgg cgatttctaa
    10621 acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga atgtttttaa
    10681 gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc ttcaagtaac
    10741 ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat gaaaaaaatg
    10801 tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat ccgctattgg
    10861 gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg attgtttgat
    10921 atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga tataccgatc
    10981 aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac acttgatgaa
    11041 acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa agagataagg
    11101 gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt tcctgtagaa
    11161 gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga aatcaaaaaa
    11221 tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag ttcacagacg
    11281 atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga cgtgatttgg
    11341 gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat gaaccatgcg
    11401 gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg gaaaacagga
    11461 gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag aactgaatat
    11521 tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg
    11581 atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta atgtggggga
    11641 ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct ttaaaaatgg
    11701 aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa agtatggaaa
    11761 aataattgta ttgggtgtta tgtataattc atggtgaact gagactaatg agaagaaaaa
    11821 ttcatattgt tatgagtgag attgtatcag atgagaatga taaattaaat aggtgcataa
    11881 aagcattaca cttaaaatac attgttgctt acattattac aatatgttaa taaggcaaaa
    11941 ataaatagat ggattgaata aacatatttt ttaatcaaat agtccctact gtgactttcc
    12001 tcagctatcg gtgatactac cgatagcatt tttttgctta taaaataatt ataagtattt
    12061 agagattttt cttctaacat tcttcaaaca agaaaaatca caaaggttat tgttattttt
    12121 atatattctt tttaagaatg attgtttttt tatagaaatt ttaaaaaata aaacacgtta
    12181 ttgatggata gttagtttgt tatttggtaa tattatagaa gttgactggt agtgatttat
    12241 ttttcgtcgt tttataaaaa tgttattttt tggaaaaatt cacaaatatg taacgaatgt
    12301 ttttctggaa tttgctgtgt taaaaagcag tatgactaat ataaatacta ttgtcattta
    12361 tgcaattttt tatggatagt aattttagag gggaatcaat gaaggaagaa tagaagggaa
    12421 aaatagttgc aaatacgcca gttagcaaaa atagcaatac atcttcaagc tttaaaattc
    12481 gagagaatca tcttggtagt ggaatcaact acaacaaaag gattaaacca attaatagat
    12541 agaattacca atggaaaaat agaaaaggta gttattctat acaaaagttg tttaattcgc
    12601 ttcggttatg aacttagtga aaccctttgc aatatatggt acagaatata gaaattattg
    12661 acattacaga aaatgatgaa gatttaatac agattgtcac tatttgtaga taccttttac
    12721 aaggaaaccg agccaataaa gctaagaaga tgattaagga gttgatggaa gacgatacaa
    12781 gttaagaaag tgaaactacg cccaaataaa gagcaaaaag agtaattgga cgtggaaaat
    12841 gcaagaaatg aaccatcgat tcggtggaca gtttattagt aatgacggtt tgagaacatc
    12901 catgacgaga atgaaggaac atcctaaatg cgtttagttg aaccaagtgc caaataatat
    12961 ggccaaataa gcgatcagtt atcgatgaat cccaagtatt ctaaactata tattcatttt
    13021 gatgctaagc attgatatat ttttttggga ttgatgaagg ttaaccaata cagggactca
    13081 ctgatgaaat tctagagtct gaccttggta tcaacgattt agtgattgtt tctaatggaa
    13141 aagtataaaa gtatataagt acattaacaa aataactatt agggttacgc tccagcttgt
    13201 gcaaggcact ggcaggagga atcgtgaaat cataattatg gttaagccac acggacaact
    13261 aaaagtcata caaagctatc gaatcaatca acagaagaca atcggcaatc attagctttg
    13321 tttacagctt acattgcaac gcttgagttg aaccgaaaat acgagggagg taacgcagcc
    13381 attttgagtt ggggaatctt tttagaaaat tatcgggtac cttgttgttt agtcaaaatt
    13441 ataaggggga gtaatagtga ggaacaaatt tcataaactg ttaagtatgt ttctagttat
    13501 gcttattgtg atcaatcaat ttgggaacat aacggttcta gctgagtctt tagcagaaca
    13561 gccagagcgt tcgctgacga tcgaagttga tcaagaggag gctgctttgg gtgagcagtt
    13621 ccagttgcga gtcagaggaa caccagacct gctaaatgca ttggtcattg ataagagtga
    13681 ggatttgatt gaaggggaag tgaagcaact caatgattac gaacgtctga ttacttattc
    13741 ttcccagcat tcaggtaaat acgaattgac cgcaagaagc ggtgatattc aatcaaatac
    13801 ggtctcaata gaaatcagtg taccagaaga aagcacaagt gagtctttta atgtaccaga
    13861 agaaagcaca agtgaatctt ttaatatacc agaagaaagc ttaagtgaat cttttaatga
    13921 agccagatca tctgagccat ctataacgca ggaatctcag attgaaagac aagaggagct
    13981 cttgaattat gacatcggca tctctgctga gcgccccact ttgccgagca ggcatacaca
    14041 aacgttaaag ggatacattc aaggaggcgc tggcgatatt cttgaagaat atggggagat
    14101 ggtcgtacga gttcctagag catcgattgg cttaattgag ttcattagag tggaggtcgc
    14161 tgatgattca cctttaaagc cactggcatt ttaccaagaa ggcacagacg ttgtttttcc
    14221 atttaccttt gattatgaag gattaaatga agccgctcag tcgtatacat tcagtatcac
    14281 gtttgaagtt gctttctttc aatcaggcgg tactgagcca aataatgact ttcaagtgag
    14341 tcttacttat gctgagcgag cagcaaacac ggaattttca attacgccta ttccttcatc
    14401 aagacccatt ttttcgaaat tatactatgg ctctagagaa atcgacagcc aaggaaatct
    14461 tgggaatctg ctgattgacg ggcaagacgt gaccaacagt aataaattta ttttgcaggt
    14521 aaattatgaa ggatcacgaa aagacggtgt agtggttacc gatgtactac cgcaaggaac
    14581 aactttggca ccgaatccga ctacatggag ttccactgga aatagagaac ctatttcagg
    14641 tggggttcgg atcgcagaag taacgaatcc aggtgaagca aacgttagaa ctcattatgt
    14701 ttccgaggaa tttgtggaca aagtatcttt taataaggaa acgaatacac ttgttgtgga
    14761 ctttggtaat ctagcagagg gtcaatcgta ttttattgaa tatgctcttg aagtgaccga
    14821 tgtcgacttg ttcaatgaag gaagacaaga ggatgggttt tctcggaatc gagcgagtat
    14881 gacctacaat aatcaggtaa tgagccgaga ctaccgcttg agaatccctg aaagtaatct
    14941 tccggcaatc tctttaagaa aaagtgtgga caaaaatgtt atcaatctca atgaaaatga
    15001 actaacgtat actctgcatg tctcgatgtt ttctggaaca agaaatacgc ttgttattga
    15061 agatgttttg gaagaaggca tggaatttgt gtcatttcta tcagatattc cctctttcat
    15121 tgatgccgaa gtcaataatg gaatagttcg tttcagtgcg aataatacga ttggtggtcc
    15181 gacaagtttt accctctctt ttaaggtcaa tgtagaggcg tatgcggtcg gcgatcaaat
    15241 tttaaacttt gcaacgcttg tgaatagtga caatcgattg aatacgagta ctgtaagaac
    15301 aaggaaaatc gatgggcgta ttaaagtaat taaaactgat gagaatggcc aaaggctggc
    15361 aggagcaaaa tttgaaattt tatcaagcga ttcacaagtg atacaagaag gtataacggc
    15421 agaaaacgga gaatttttaa gtgaaccgct tcctgtaggt agctatcaag tccgagaaac
    15481 agaagcaccg gaagggtatc ttttagatgc gacggtacat gatgttttta taaatgaata
    15541 ctccatagaa gaagtgacgt taaccattga aaacatcctt gatacgggtg gtgttgaact
    15601 aattaaacat gctgaaggca gtggtgaagt ccttcaagga gctgtttttg agttacagaa
    15661 ccgagaagga gaaacgctac aaaccggt
//
LOCUS       DAFCLG010000061        14258 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFCLG010000061 DAFCLG010000000
VERSION     DAFCLG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.061"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            272..2743
                     /locus_tag="LND56_001701"
     CDS             272..2743
                     /locus_tag="LND56_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6743624.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2846..4582)
                     /locus_tag="LND56_001702"
     CDS             complement(2846..4582)
                     /locus_tag="LND56_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBK6743625.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4579..5283)
                     /locus_tag="LND56_001703"
     CDS             complement(4579..5283)
                     /locus_tag="LND56_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743626.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5414..5917
                     /locus_tag="LND56_001704"
     CDS             5414..5917
                     /locus_tag="LND56_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBK6743627.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5877..6215
                     /locus_tag="LND56_001705"
     CDS             5877..6215
                     /locus_tag="LND56_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBK6743628.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6236..7654
                     /gene="ffh"
                     /locus_tag="LND56_001706"
     CDS             6236..7654
                     /gene="ffh"
                     /locus_tag="LND56_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK6743629.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7766..8344
                     /locus_tag="LND56_001707"
     CDS             7766..8344
                     /locus_tag="LND56_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK6743630.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8348..8869
                     /locus_tag="LND56_001708"
     CDS             8348..8869
                     /locus_tag="LND56_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6743631.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8948..9502)
                     /locus_tag="LND56_001709"
     CDS             complement(8948..9502)
                     /locus_tag="LND56_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6743632.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9755..10030
                     /gene="rpsP"
                     /locus_tag="LND56_001710"
     CDS             9755..10030
                     /gene="rpsP"
                     /locus_tag="LND56_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK6743633.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10042..10293
                     /locus_tag="LND56_001711"
     CDS             10042..10293
                     /locus_tag="LND56_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBK6743634.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10418..11209
                     /locus_tag="LND56_001712"
     CDS             10418..11209
                     /locus_tag="LND56_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743635.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11379..11900
                     /gene="rimM"
                     /locus_tag="LND56_001713"
     CDS             11379..11900
                     /gene="rimM"
                     /locus_tag="LND56_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK6743636.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11890..12651
                     /gene="trmD"
                     /locus_tag="LND56_001714"
     CDS             11890..12651
                     /gene="trmD"
                     /locus_tag="LND56_001714"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK6743637.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12787..13134
                     /gene="rplS"
                     /locus_tag="LND56_001715"
     CDS             12787..13134
                     /gene="rplS"
                     /locus_tag="LND56_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK6743638.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13392..13685
                     /locus_tag="LND56_001716"
     CDS             13392..13685
                     /locus_tag="LND56_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBK6743639.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4770 a         2566 c         2918 g         4004 t
ORIGIN      
        1 gagagactcc tttggtagga tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc gccttcgatt ttccaccaac
      121 acaaaatatt atagaaccct ttttttttgc tatatgacag gaatgaaagg tgtttgcaag
      181 cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag tttggaaata
      241 tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa atcgaagtaa
      301 aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc catcgtataa
      361 agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag caaataattc
      421 agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat ttacgtattt
      481 caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct ataagaactt
      541 gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg aaattcgatc
      601 caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg tcatacgaga
      661 cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg ttattttaga
      721 gactggatat caaattccag cagatgcaga ggtcgtggaa gggaaactgc aagttaatga
      781 ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggtgatc cattaatgtc
      841 cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg gagcagattc
      901 gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac agtcggagat
      961 gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc ctatcggtat
     1021 cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc
     1081 aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga cgacgattgc
     1141 attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca atatgaaaag
     1201 cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga
     1261 aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc
     1321 agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag acaatgcaac
     1381 aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc accatgttat
     1441 tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg tgtatgtttt
     1501 aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag agtttgtttc
     1561 tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga
     1621 accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta atcctatcag
     1681 aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca tcaaagtgat
     1741 ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga tttcacatgc
     1801 tgatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa
     1861 atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc aattgataaa
     1921 gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag ccatgaaaga
     1981 agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg cctcacaagt
     2041 ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag gacggcgcgt
     2101 tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt tctccttttt
     2161 gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat cacaaatcac
     2221 attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag aagaaaacaa
     2281 aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc caggtggact
     2341 aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga atctaaataa
     2401 aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc tcgttctcta
     2461 taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct gtgctagcgg
     2521 taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta tctcagctgt
     2581 atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta ggcatttgac
     2641 cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg
     2701 cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa
     2761 aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc atccaacttt
     2821 aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg aaagtcgaac
     2881 ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg tagtctttta
     2941 caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt
     3001 aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca aagtcttgga
     3061 cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg tcgctttttg
     3121 aatattggac agcattttca atcagatttt tgataattgg ataaaggata tctgttttag
     3181 tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg
     3241 gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc tggatctcgt
     3301 aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta tctaaccgga
     3361 tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac
     3421 catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt aattcgtgag
     3481 agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc gttaagtcat
     3541 acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc aaactcaagt
     3601 ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga tgctcaggag
     3661 tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg taccgctgtt
     3721 caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc agtagtaaac
     3781 gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt aacgtatata
     3841 attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg ctcaacgcat
     3901 taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact tccgcctgat
     3961 taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc tggttttttt
     4021 gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa
     4081 agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac gcttgtcggt tctgcgatac
     4141 gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat aaaagatcct
     4201 cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc aatacggctt
     4261 tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg tcaaataaga
     4321 tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg aattcttcca
     4381 aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg atcagcagtt
     4441 gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga aaataatggg
     4501 aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag aagagacgtt
     4561 cataaatttt tttccatttc atcgtttggg ttcctgaaac ttatagccaa atcctctaat
     4621 agtcaccaag tactttgggt gttttggatc tcgctcgatt ttttctcgca aatgactaac
     4681 gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa
     4741 tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta attcaaattc
     4801 tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg tcaaatcagc
     4861 agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt gcttacgagg
     4921 ttcaattcgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac taaatggttt
     4981 ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt ggtcatcttt
     5041 agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct gagtgatctc
     5101 catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg attgttctaa
     5161 tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc cttctttttc
     5221 caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta gtattttttt
     5281 cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag aatcagtaca
     5341 aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc tgaatagaaa
     5401 ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct gtcgctatga
     5461 tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag aagctgtcat
     5521 ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg aaatcattgg
     5581 tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa gcggcgaaga
     5641 tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc aggaaaaaca
     5701 cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt cgatttatga
     5761 cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt attttattca
     5821 gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg agcagaatgg
     5881 aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc ttattgacag
     5941 aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta ggtgagatcg
     6001 cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg acaagcaaga
     6061 tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt cgagaacagg
     6121 tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa aaactacttg
     6181 aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag aaaatatggc
     6241 atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa aaaaaggaaa
     6301 agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct tactagaagc
     6361 cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg
     6421 ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga
     6481 attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa aaataccaac
     6541 tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg gtaaattagc
     6601 caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg acgtatatcg
     6661 accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc ctgtatttga
     6721 tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt tagcaaaaga
     6781 aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt
     6841 gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt tgcttgttgt
     6901 cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc agcaattagg
     6961 tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg cagcactatc
     7021 gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa aattgactga
     7081 cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg gggatatgct
     7141 gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag aacttgcaca
     7201 gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg atcaagtgat
     7261 gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc aagtccctgg
     7321 aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga tggtttattc
     7381 tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc gtcgccgaat
     7441 tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac aattcaagga
     7501 atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag gtatggacca
     7561 aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa
     7621 gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct tcggtacaga
     7681 ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta ctaatacaat
     7741 tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa cagaaagtat
     7801 gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa agctgttccg
     7861 aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt taaataaaag
     7921 tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa attatggaga
     7981 aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga
     8041 agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc
     8101 agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc attcacaaga
     8161 agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa agaaaagagg
     8221 atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg ggatcatcaa
     8281 tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca gaaagggcga
     8341 ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct atttggcaac
     8401 gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa ctacttatga
     8461 agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc aggaagcaaa
     8521 aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt tttccaccca
     8581 cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa
     8641 aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa
     8701 accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc ctgtattatt
     8761 aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga
     8821 cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga agtaaataaa
     8881 ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt tattaccgtt
     8941 atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa atcgtttgga
     9001 agaaacttaa tgttccttca acgatattac cattttcatc aattagattc gcaacgtttc
     9061 cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt gattggcgta
     9121 gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg actaaacctg
     9181 gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa gcatttttca
     9241 atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg tctagtcccc
     9301 tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt tcgagatcct
     9361 cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct tcaggaagca
     9421 atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa ctaccaataa
     9481 aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat aataatctta
     9541 caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac atagaagata
     9601 gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa aaaaatcgaa
     9661 tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt tacactacat
     9721 cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc gtttaaaacg
     9781 tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac gttctcctcg
     9841 tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt
     9901 agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac cttctgacac
     9961 tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc
    10021 aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac aatcgttcgt
    10081 ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa agacttttac
    10141 gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa acaagggcgt
    10201 gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg
    10261 cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc gaattttcga
    10321 gtgcagcttt tttgtttttt ttattaattg aagaaatgat tatttggcaa aacaagcaag
    10381 aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa tgttatttat
    10441 tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag acaatacaca
    10501 tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta ccaaaacggc
    10561 ttccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg ctttagacca
    10621 gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct cttcaggcta
    10681 tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt acagaacaga
    10741 tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg caacctatct
    10801 atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc catttgagat
    10861 cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc agcaaggtgc
    10921 agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa
    10981 taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt atttggaaga
    11041 aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg gtgatacgac
    11101 gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga tcactcatca
    11161 agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa atcagcgcgc
    11221 tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata gcacgccgca
    11281 tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc ttcacacatg
    11341 ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac ttgaatgtag
    11401 gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca acaacggatt
    11461 tcccagaaga acgttacaaa aaaggaacgg tgctaacact attccaagac ggaaaagcac
    11521 ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta ttgagttttg
    11581 aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta aaagtgtcaa
    11641 aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc atcggtctgc
    11701 atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca ccaggtgcca
    11761 atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca tatatcgctt
    11821 ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt ccagaaggac
    11881 tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga aggtcctatg
    11941 ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa tatctctaac
    12001 ttccgcgatt attcagataa caaacatcag acagtagatg attatccata cggcggtgga
    12061 gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat tgaaaaagct
    12121 gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg attcgatcag
    12181 aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg tcattatgag
    12241 ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg agattatgtc
    12301 cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg gcttttgcca
    12361 gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg tttattggaa
    12421 catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga agtattgaca
    12481 aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag aaggacctat
    12541 caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac gaaattttta
    12601 gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg actttttcta
    12661 aaatcttctt tacagtaggt tgataatatg ttagtatttt atggtgagtg caaaagcact
    12721 taactattac gatattccgc tgtgagaaaa gctcataaga atatttggaa taaggagaag
    12781 aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc tgacatccca
    12841 gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa
    12901 cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat cagcgaaact
    12961 tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc attgcataca
    13021 ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac
    13081 tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatccgtcg ttaatcattg
    13141 ttatcagact gagaaagaac ccttggtatt caagggttct tttttcttag catgtaattt
    13201 gaacaccctg atccagtttt ttctttaatt attggtaggg gagaataatg gattagaata
    13261 aatctaccta taagtagtag taaaaaaaag agcactatga taatatcttt cttaagagta
    13321 accgctttaa taaaaaatcc agaatattct aacataaatg gagtattctt agaatcaaaa
    13381 ggagaaagaa gatggtttat acttatacgg agcaagaaaa gatgaaagtt ttagacacat
    13441 tcatgaatga tggaaaagta aaacaagtac caagttcaac aaaaaagaaa tacatcctat
    13501 tgaaagaagt cttgaaacgc tttgaccatg gagtgactta tacagaaaca gaggttaatt
    13561 cgatactgct gaacgtattt tctagtggcg attatgtcga acaaagacgt tatctaatca
    13621 catttggctt tttcaaaaga agttcagacg gtcgggcata tcagatgatg ggaattgaaa
    13681 attagaagtt ttaatttata ctcattgaaa aaagttcaag tatgcgatat tggttctaca
    13741 ccgcatactt gaactttttt tgtcatctgt atctataata caaagccaag aacgttattc
    13801 aaaatatgga tagctatgat aaaagctaat gagcgattcg cagttaaata agcaatcgtc
    13861 agtaaaatcg ttccaggagc gtacatgaaa aaatcaattg gatgcgttac atgaataaag
    13921 ataattctct tttatttgta cgaataaata ccaaaacgta cctcctaaaa ttagattttg
    13981 gacagatgcc ctttaaaaag ataacaagat ttcgttcttg aaacaatata tatgctttta
    14041 ttccaatgag gttgtgaaaa cagtattttg tcctgagcgt tagaaaattg aaaatgggga
    14101 atagcaactc gtactttacc attttttaag tgaatacaag atcagctaat tgaacaccgt
    14161 ttattcgtga tgagggacac aacactgcag tcgacacatt tctcttttat gttcaaagat
    14221 aacaaatatt taaaacaaag aaaaatagta gataatcc
//
LOCUS       DAFCLG010000062        14118 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFCLG010000062 DAFCLG010000000
VERSION     DAFCLG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.062"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..556)
                     /locus_tag="LND56_001717"
     CDS             complement(<1..556)
                     /locus_tag="LND56_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK6743640.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="LND56_001718"
     CDS             complement(572..1051)
                     /locus_tag="LND56_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK6743641.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="LND56_001719"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="LND56_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="LND56_001720"
     CDS             complement(2098..3204)
                     /locus_tag="LND56_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6743642.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="LND56_001721"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="LND56_001721"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK6743643.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="LND56_001722"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="LND56_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK6743644.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="LND56_001723"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="LND56_001723"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK6743645.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="LND56_001724"
     CDS             8175..8873
                     /locus_tag="LND56_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6743646.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="LND56_001725"
     CDS             complement(9041..11680)
                     /locus_tag="LND56_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6743647.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="LND56_001726"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="LND56_001726"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK6743648.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="LND56_001727"
     CDS             13523..>14118
                     /locus_tag="LND56_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6743649.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DAFCLG010000063        13937 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFCLG010000063 DAFCLG010000000
VERSION     DAFCLG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.063"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(334..696)
                     /locus_tag="LND56_001728"
     CDS             complement(334..696)
                     /locus_tag="LND56_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743650.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="LND56_001729"
     CDS             complement(770..1150)
                     /locus_tag="LND56_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBK6743651.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="LND56_001730"
     CDS             1214..2407
                     /locus_tag="LND56_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6743652.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="LND56_001731"
     CDS             complement(2484..3068)
                     /locus_tag="LND56_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6743653.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="LND56_001732"
     CDS             3269..4267
                     /locus_tag="LND56_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6743654.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="LND56_001733"
     CDS             4379..4888
                     /locus_tag="LND56_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK6743655.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="LND56_001734"
     CDS             complement(4918..5577)
                     /locus_tag="LND56_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBK6743656.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="LND56_001735"
     CDS             complement(5579..6343)
                     /locus_tag="LND56_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBK6743657.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="LND56_001736"
     CDS             complement(6351..7028)
                     /locus_tag="LND56_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBK6743658.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="LND56_001737"
     CDS             complement(7065..8450)
                     /locus_tag="LND56_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6743659.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKIPKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="LND56_001738"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LND56_001738"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="LND56_001739"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="LND56_001739"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK6743660.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="LND56_001740"
     CDS             9389..10246
                     /locus_tag="LND56_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743661.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="LND56_001741"
     CDS             complement(10327..10662)
                     /locus_tag="LND56_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743662.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="LND56_001742"
     CDS             complement(10729..12099)
                     /locus_tag="LND56_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBK6743663.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..12713)
                     /locus_tag="LND56_001743"
     CDS             complement(12177..12713)
                     /locus_tag="LND56_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6743664.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13022..>13937
                     /locus_tag="LND56_001744"
     CDS             13022..>13937
                     /locus_tag="LND56_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6743665.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMT"
BASE COUNT         4262 a         2787 c         2267 g         4621 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct tagggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
    12721 tccgtctctt ttcatctacc cataaataaa taacataaat aattctaaaa atattattga
    12781 taattatcaa aacatattta gttatatcca ttaaaaatca atttttccgc cttaaaaaca
    12841 agaatatctt gacaaaatac cctaggaaat aaacaataga ataaacaagg taacaaaaaa
    12901 attaaataac gttatataaa aaagaaggcg acactctgtg agaataacac agactgaaat
    12961 gactttccac tcacagtttt tgtctaacta tatacaaaaa cagaataaaa aggaggtatt
    13021 tatgctaatt gaagcaagaa aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa
    13081 taattgttct ttacaagaat tatccaatga cttacatatt ccaaaacgga caatcaaaga
    13141 actgatccga aaaataaatg tatcactcaa gcaattttta ttgattgatg aatttattta
    13201 ttctaatcat aaaggagaga tccaaaccaa agaaaccgat caaactaaaa agttagctgt
    13261 tttttctaag ttaaaattat gctacctaaa agaatccaat cgttttaact atttgatgtt
    13321 actaatcaat tatccacaaa ctgctgtacc aaaaaaatat ttacttgaac aactttatat
    13381 atcgccctcc tatctcgaaa aactgacaaa acagctcaat cattccttaa aaaaattcca
    13441 tttacggatc ctatctgtaa aaaattgctg tactcttgaa ggaaatgagt tatctatccg
    13501 tctctatctt tacttttttc tttcagatac atttggaggt aacgattggc cattttcctc
    13561 acttgaatta aatgcactaa aaatgaacag tacagatgag caaatagaaa aaatttcaaa
    13621 acaagaaaaa aatatctact tgctgttaac tttattcata ttacgaaaca aacaacaggc
    13681 atattttcca aaaacttctt ctgaaatcac tgaattgatg gacttattgc agcatacaca
    13741 tgattattca gaatacttta cttctttttc agtgagtcgc ccttgtgaag agacagaaac
    13801 agagagagcc tactttaatt attttttcta ttgttttttt ccaaatcaaa tctcttttcg
    13861 acaaaaacag gagattggtt ttttattttc cacaagcaaa aaccttattt gtcgacaagt
    13921 cggtcaattg atgactt
//
LOCUS       DAFCLG010000064        13793 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFCLG010000064 DAFCLG010000000
VERSION     DAFCLG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.064"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..318)
                     /locus_tag="LND56_001745"
     CDS             complement(25..318)
                     /locus_tag="LND56_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBK6743666.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(346..1011)
                     /locus_tag="LND56_001746"
     CDS             complement(346..1011)
                     /locus_tag="LND56_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBK6743667.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1151..1783)
                     /locus_tag="LND56_001747"
     CDS             complement(1151..1783)
                     /locus_tag="LND56_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743668.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1790..2857)
                     /locus_tag="LND56_001748"
     CDS             complement(1790..2857)
                     /locus_tag="LND56_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6743669.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2854..3585)
                     /locus_tag="LND56_001749"
     CDS             complement(2854..3585)
                     /locus_tag="LND56_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBK6743670.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3753..4232)
                     /gene="greA"
                     /locus_tag="LND56_001750"
     CDS             complement(3753..4232)
                     /gene="greA"
                     /locus_tag="LND56_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK6743671.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4368..5543)
                     /gene="mltG"
                     /locus_tag="LND56_001751"
     CDS             complement(4368..5543)
                     /gene="mltG"
                     /locus_tag="LND56_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBK6743672.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5708..6868
                     /locus_tag="LND56_001752"
     CDS             5708..6868
                     /locus_tag="LND56_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6743673.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6908..7855)
                     /locus_tag="LND56_001753"
     CDS             complement(6908..7855)
                     /locus_tag="LND56_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK6743674.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7988..9379)
                     /locus_tag="LND56_001754"
     CDS             complement(7988..9379)
                     /locus_tag="LND56_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK6743675.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            9505..9915
                     /gene="tagD"
                     /locus_tag="LND56_001755"
     CDS             9505..9915
                     /gene="tagD"
                     /locus_tag="LND56_001755"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBK6743676.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            10095..11384
                     /locus_tag="LND56_001756"
                     /pseudo
     CDS             10095..11384
                     /locus_tag="LND56_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11451..12878)
                     /locus_tag="LND56_001757"
     CDS             complement(11451..12878)
                     /locus_tag="LND56_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK6743677.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(12875..>13793)
                     /locus_tag="LND56_001758"
     CDS             complement(12875..>13793)
                     /locus_tag="LND56_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBK6743678.1"
                     /translation="RIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQLYFQTWHGT
                     PLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKKYVSAFNIVPSKILEVGT
                     PRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFKKQQQ
                     ELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEIDVLITDYSSIF
                     FDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDVFKNQQEKLLKS
                     SQELLDYIGNYETGASTEKVLELIERDIK"
BASE COUNT         4502 a         2652 c         2053 g         4586 t
ORIGIN      
        1 aaacttgtgt caccctctct ttttttattg ttttttaaag tgataggttg gctcctccag
       61 gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat ctgcttcctc
      121 gataaaaaaa agtgtcccat ccatttccac tttctttaca ggatgttctg gctgttcaac
      181 tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt ttccaaaaaa
      241 acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt gtgcttttgg
      301 cgttaccgtt aattccatcc caacaaccta ctttctcttt tctttctatt gattcatctt
      361 atcgtctaga atatccacat cgcctgtctc tccaatcacc agtagattat cattttcgcg
      421 aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc gtatggcgac
      481 tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt tgaaaaattt
      541 aggattggag actcggattt ctgctaaaga atatttatct gacagctcta agtagtctag
      601 aatattcttt gatactaagt tatgtgcaat ccgtatcccc atgtctcgct cagggtgtac
      661 gacacgatct gctccgatct tctctagtac acgggcatgg tactcatttt gggcttttgc
      721 taaaatattc ggtacgccca tttcctttac catcagagta actaagatac tggcttggat
      781 atcttctcca atagcgacaa ttacatgatc gaaatttctt atacctaatg aacgaagtgt
      841 catctcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat attcattgac
      901 tcgatcttca ttactgtcaa tagccagtac ttcttgtcca gcttcgatca gtgtacggca
      961 gatgcttccg ccaaatcgtc caaggcctac aattgcaaaa ttttgtttca ccattttcct
     1021 cctttagtat cttcttttct cagtataaca aagcctttcc aaaaacaaaa gcaagcatca
     1081 ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat tatcctcttt
     1141 atcatcgttt ttatttcacc aaaccatgtt taaacgcata gatagctgct tgagttcggt
     1201 cttctacctc taacttagct aatatattcg acacatgcgt tttaactgtt ttcagtgtga
     1261 taaacaattc atccgcgatt tcctgattac ttttcccttc agaaatcagc atcaaaattt
     1321 cattttctcg attcgttaat tcttcatgca atactggatc atttcgccga ctcatttttt
     1381 ccatcatttt tgttgtcact tcaggttcca atactcgctc gccgcgttgc gtagccctta
     1441 tagcatcagc tatttcatgt gcggtagacg tcttcaacaa atatcctgct gctcctgctt
     1501 caattgcagg ataaactttt tcatcatcaa taaaacttgt gacgataatg attttggctt
     1561 ttggacaatc ttttaagatt gcttttgttg attcgatgcc atccatttct tccatgacta
     1621 gatccattaa aatgacatct ggtcgaagtg ccatcgcctt ttcatagcct tgacgtccat
     1681 tttcagcttc tccaatcact tcaatgtctt cttggataga taaataagaa gagacgccta
     1741 agcgtaccat ttcatggtca tctactaata aaacttttat catctttatc tatgcctcct
     1801 tcatcaaagg gaccttgatc tccacgctcg taccttgccc tttaaaacta atgattttta
     1861 ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa cttcccgttt
     1921 tggtttcatt catattaaat cctgtcccgt cgtcaatgat acgtaataaa agattgttat
     1981 ctattttatg caaatagact tctaattcat tagccttagc atgtcttaat gtgtttgata
     2041 acaattcttg aacgattcgg aacaagtgat cctcaatgga actagttaat tttacatctt
     2101 ctacatccca ttttagcgaa atctggattt tattctgcaa ttccttcaat aactgctcaa
     2161 tgccttgttt taaacttttt tcttctaaat tgactggacg cagatgaaga agcagcgcac
     2221 gcatctcaga ctgggaagcg ttgatgatct ctgccaccat tttcaactgc ttacggaaca
     2281 tttctggtgt ctcacttttt tctgcttgct ccgtcaatgc cgacatcatc atcatcgctg
     2341 caaataattg ctgtgaaact gaatcatgaa gctcacgagc taatcgatgc ctttctaatt
     2401 ctaaaatctc ttctttggtt tgtccatcta catattgagg acggctagtc ataatctgta
     2461 attcactgga tacttcgatc attttttctt ttattttagc aatttccata tcaatatctt
     2521 tgatgtacag atcatccgac gcgcttggga tcggctgatg caaaagtttg gattcataat
     2581 tgccagaaga aagtgcacga agcttttctt cgattttccc gtattttgcc ttttgaataa
     2641 gtgataacaa tagaaatacg atcaatccta cacctaatga aattgccatc aaatggaaga
     2701 taagcggaac gtataataaa cgcgcacgca gcaattctcc ccaccaatga ctttggttac
     2761 tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca atccccgaat
     2821 aaacagctag cattgctttg gatattcttc ccatcatatt cgtatcacct cgacatctcc
     2881 aaccaaggta ttggtaatga tcttcaatct gcgaggattt tcatcataat cactgctgca
     2941 gattttgatc tgttcgtttt ttaaggaaaa ctgctcttct tcaaacaaga catttcctac
     3001 taatgtcgca tgctctaaac gaatagccac tcccaggggt actaagatcc gtgttctgcc
     3061 tatccctttt cttacaatca caatattatc atcttttggc aaaagtgtat tacccaaatc
     3121 gatgatagta tcaccagaaa tgatggcaat gttgatatca tcccattcat agacgtcgtt
     3181 tccaatacgt tggttaccaa ataattgctg tcttttacgc tcattattat gtgtttttaa
     3241 ttgctctgtt tggaccatca tgatctgttt ttttcgccaa aaagcatttt ttgtcaaatc
     3301 tattcctgaa atctctacac ctttcagccc gataaataaa acggccaata caaccatcat
     3361 ccataaagca ggactgttga ctaagctaaa aaaaatgacc aagctcccta agatcaattg
     3421 aaaattttga aatttagtac gaggatattt gcgcatagct agatatatat tgaaaatacc
     3481 gaatattact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag caaataacag
     3541 cagcagtgct tcgactacca caaaaaaacg ccaagaacta ttcatcgctt tttctccctc
     3601 ctgacacatt ctcatttcat catacctatt tttttgactg ttggctatag gactttagat
     3661 gtaagatcgt tcagtgaaac ctgcaatact caaagagaaa agatctagag tgcgtcatca
     3721 gtaagatgaa ctctctagat ctttcagtca gactacgctt gttctacttt gacgatttta
     3781 actaacatat cgccacctgg tgtagcaatg gttacttcat catttttacg tttgccaatc
     3841 aatgcttgcg cgataggaga atcattggaa atctttccag aaaacgggtc tgcttcagca
     3901 cttccgacaa tcgtgtactc ttcttcatct ccatcaggca actcgatgaa cgtaactgtt
     3961 ttaccgatcg atacctcatc tgcatctacg ccgtcattat cgatgatttg tgcaaaacgg
     4021 atcatatttt caattgttgt gattcttcct tcaacaaatg cttgttcatc tttagctgat
     4081 tcgtattcgg agttttcgga aagatctccg aaaccgcgag cgattttgat tcgttcaatg
     4141 atttctccgc gtttaactgt ttttagttct tctaattctt tttctaattt ttctttacct
     4201 tctagagtca ttggataaac tttctctacc atgtgtaacg tctccttttt tcgataaaat
     4261 accgcaaaag gtaatctttt gaggaatata gtccaaaaaa gattaccacg cttttcttaa
     4321 aatgtaaatg tttttctaaa aatgatcaat gactagcccc tgtttttcta agaattattt
     4381 acatattttt gcactagttc catatgttct tcatacgttt tagcgaaata aacatttcca
     4441 gtgctcgtat ctgctacgaa ataataatag tcattttctg ctggttctaa tactgccttg
     4501 attgcctttt cgcttggatt gtcaaaagga ccaggcccat agccggtatt ggtatacaaa
     4561 ttgtatggtg aatctacagc tgtatcttca tacgtcacca attctttatg ttctcctaat
     4621 gcatataaaa cagagatatc tgactgaagt ggcatatttg ccttgattcg gttaaagaag
     4681 acttgtgcaa tatttttacg atcattttct tttacgcctt ctttttcaac tagagaagcc
     4741 aaagttaaaa cttcttgtac agtcaaattt ttctgtttga tagctgaata atagttttgc
     4801 atgaccgtat ttgtcttgtt caccatctca atcacaagat ccttaagcga agtactcgtg
     4861 tagtaatcat aagtagctgg gaataagtag ccttccaaac ggtattttgt gttctcagct
     4921 ttagaggcac tttccaacaa ttctgggaat ttttgataca actcgttaaa gaaagtctca
     4981 tcatttacta aatctaaaaa ttctttcttg tctttacctg ttaccttcgc tacccgctca
     5041 gcaatctgat cgatgtcgta gccttctgga atggcaattt tcccatctgc aacttttgtt
     5101 ggctcagatg tgccgccttc ttgcaactgc ttgccaatct catcaagagt catattaggc
     5161 gctaactgat aataaccagc ttgaaagcca gttaagtttt taaattttgt ataataattg
     5221 aaaacaatcc cacttttaat aatattgtct ttctccaaaa tttcgccaat ctgcttatta
     5281 gaagagccgt ttgggatttc cacttggact aattggtcgt ccgatttatc taaaggcttc
     5341 aacccgctat ctacataacg atatacggta aacccgagaa atccgccaat aatcaataat
     5401 gtaagagcga tcactaaaat gatcttcctc acgatcttat cttctttttt acgcagattt
     5461 ctccgcttgt cgattatatc tgctgtagct gccttactgg cttctttttt gtctttgctt
     5521 gggtgttgtt tgtttcctga caaaacaact cctccttact atcatcttcg taactctgtt
     5581 tattatacat gaaatttcta ctgccgtgaa ttattttacc aaatattatg tcaaaattgt
     5641 aagtggcaaa tgctcttttt gaggattaca atgaaaaata gttaaaaatg gatagaaagg
     5701 atttaatatg gcaaaaggaa aacatcaaaa aaaacagcag atctctcttt cttttctcgc
     5761 agttttgttg ctggctgtca gcttcttttc aacaggcatc attacaggat ggcaattaac
     5821 aaaacaacct caaccacaaa tttataaaac ggaacccgca caacttgctt ccaatttaac
     5881 atctaatgag cagatgcttg ccagcaaaat tgatcaatta ctgcaaaaat cagattatat
     5941 cggttcgatt tatgtacgca aaaataatcg agtgatatta gaaaaaggat atgggtacgc
     6001 aaatctacgc ataaaagaat cgaattctcc ttctctttac tatcaaatcg gttcgatcca
     6061 aaaagcgatg actgctttac ttatcctaaa acaaattcaa tctggcaaga taacttttga
     6121 cacaaagcta tctgaatttt atcctcagat tcctggaagt cagcaaatct caatcaaaaa
     6181 tatgctttac atgcgctctg gtcttcatcg aaccgcttct ccaaaaaaac ctatgtcaga
     6241 tagtgagatc attcagtttg cccttcatca tttgaaattt actggttatg atacgtatca
     6301 ttatgaacca ttaaattata cattgcttac aggggtattg atcaaactaa tgaatcagcc
     6361 ttatgaaact cttttaaaaa aagaaatcat tcggccactc catctagaac atacagattt
     6421 ttatgaaaat gtaaaaaatt cccctcagca tgctgtatct taccaaatgt ctgcaactga
     6481 cgattattca aaagcattgg tcgagcctga aacagacatc cgaaatgaat taggcacagg
     6541 aaatataagt atgtctgttt atgacttgaa caaattcttt accagtgttc tgtctggcga
     6601 tattatacca aaagaactac ttttctcatt gtggcaaaat aatggtgata aacatcctta
     6661 cagcggcggt gtttacagtg gaaatgatta tatccttgct cagggaaata tcaatcgatt
     6721 ccatgcagta gcggcgatga aaaaagacac aaaagatgca atcgtcatgg aaagtaatat
     6781 ccaatctgat aaaaacatca aattgccggc cactgattta agaaatcaga tttacgaact
     6841 gatggaaggt gttaatttaa aaagctaagt cgagacaaaa aaaccagcag gatgctggtt
     6901 ttttgtttca atcgatttct gtctttaaat aacgttccat tccgttcaat atatcaaatt
     6961 tgggttgata acctaaagaa cggatctttg aaatatccgc taaagaatca cgaatatccc
     7021 ctgaacgctc cgcttcatat ttaagcgcta gctctgttcc taagatttga tcaattgaat
     7081 gaatcaattc aagtagcgta gtgctttttc ctgtaccgac attgaattgc tgtcctaaag
     7141 ctttttcttc cttagcaaca agtaaaagtg cttgtataac atcttctata taaacgaagt
     7201 ctctagattg gctaccatca ccaaataaag taaattctgt tttttctcct gccaattgtt
     7261 ttttgtatcg gtccactaga atcgaaataa ctccgctata tggagagtta ggattttgat
     7321 ttggaccata aacgttaaaa aatcggacag cactagtcgg aacatcatat aaatgacagt
     7381 aatccaaaac atattgttct gatgcaaact tatcgatggc atatggtgtt aaaggacgga
     7441 tgaccgattc ttcttttttt ggcaacgttg gttcatcgcc ataaactgca gctgatgaac
     7501 taaataccaa tcgttttaaa ccagattgat attttcgtac caattcaagc aataaaagcg
     7561 cactgtcaaa atttacacga tgagtttcta gaggacgttc tacagaatct gccacactag
     7621 ctacagcagc taaatgaaaa atataggcaa actgatactg tttcatgatt ctttccatca
     7681 attgaggatc tgatacatcc ccctcaatga aagtcacttg tttctccata tccaagtttt
     7741 ctttttttcc cattgataaa tcgtcgacta ctacgatttt gttttctttt cccaaataat
     7801 tcgctagcgt tgaaccgata aaaccagctc caccagtaat caatatagta tccatcaaat
     7861 tcactccaat tatgcattct catacttaat aaagttagtt taccattttt atattgtaaa
     7921 aactataaaa tttgttgaag ttgggataac tagttttctt ttctacttta tatacctaaa
     7981 aatagtttca attagttact ccttcttctt tagatatagt tttttggata gaacccagat
     8041 atccccaaaa aataaatgaa cctatcgtac taaccaaaat gagttctgtg ataaataatg
     8101 ccgaaactgc tattgtaaga atcattaaaa tagcaaaaaa gtgcttatct tttactagat
     8161 ttggttgtgt gaaacaataa cgcaaagttt taaagcattg gataaataag aaggttaaca
     8221 atgtcaatag acctaatcct cctgtagcag ccataactaa gaaaataaaa ttatgagcag
     8281 ttaaagtatc ttttcccatt aatgtatatg gtagtttatc atgtgcgtat tcaatatagt
     8341 atcctggaga accgccggat atcggcttac tcttgaatat ttcaattgca cttttccaaa
     8401 gtccaaacct taaattagaa acatccccgc catcagtgtc agggcgtttt aaatcaactt
     8461 tattgctgct agttgttcct gatgaatcag atatatcttt ttttatttct gtaatatgcg
     8521 gaacatgtgc ccatgcttga gcagttatat ctgttacata atacatcgca attatactta
     8581 gcaatcctac tactaatgca gttagccatc tttttaaagt attagtttta agaaaacgac
     8641 tactataact tgtaaagaaa ctaaaaacaa aagttgctat taataaacaa actaatgtgg
     8701 tgcgtgagcc cgataaagca ataaacgaga attgaataaa aatattaatt gcaaaaaata
     8761 ttttaaatag aagatttaaa tgcttgtaag tgaccaaata atatatagca ataatgatga
     8821 caattaatga tataaccgca ccaaaatttg gatctgtata tacaccaaat aaacggttgc
     8881 ctaaccaccc taaacgaact gggtaatact tatattttat tggaattttt atgccatatt
     8941 gaatgacaaa ccagtataat gatactaaat tagctaaaaa ccataccaat gaaagtatcc
     9001 aagtaaatac tttgaaaaaa aagttactac tgtagtcttt gccaaactga taaatcataa
     9061 agaaaaagat taattgccac atgattaact tgatattttc gctatatcca tattttaaat
     9121 tcattaaact gcttactagc atcgcgccaa taaaagcaat taatagccaa tcatattttt
     9181 taagattttt ttctctaaat gcaataattg catctataat gatcaagaca actcccgcta
     9241 ttccagcagg ccaaaaaagt aaagtatcat atctagtagg taatagtaaa tacgggccta
     9301 ttgctcgtaa cattagaata aaaccaaaat aaataaaata ccaacctttc acatatttta
     9361 aaaatttttc attcaacatt tttcctccat ccacctattt ctcataaaca tttcttatcc
     9421 tttttaggat aaactggttc tttcaattct aacaaaaaaa tgctaaacta acaatgtata
     9481 ttaatgaatt tcgagaggtg aaagatgaaa agagtaatta cttacggtac ctttgatttg
     9541 cttcactacg ggcatatcaa tttactgaaa cgagcaaaac aatatggaga ttatcttatt
     9601 gttgcccttt ctacagatga attcaattgg aatgcgaaac aaaaaaaatg ttattttagt
     9661 tatgaaaaaa gaaaacagtt acttgaagct attcgctacg ttgatttagt tattcctgaa
     9721 gaaaattggg aacaaaaagt aaaagatata aaagaatacc atgcagatta tttcgtgatg
     9781 ggcgatgatt gggctggaca atttgatgaa cttgaacaac taacagatgc acaagtaatt
     9841 tatttaccaa gaacacctga gatctctaca actcaaataa agaacgaact taattcttta
     9901 aatagtgata aataatattt tttaaacaat gaaagaacaa tatatctgga taagacgtaa
     9961 aaaaatggct ctataatttc taggactgat agaattttct gtcgatccta gattttcttg
    10021 taaaaaaaac agacatacaa aaatttcgat aaaatagagt cgtctaaaac atcaatctga
    10081 aaggaagatt ttgtatatcc atttctcgtt ctatcaaaga gcacttcact attaaagata
    10141 aaaatattga aattaattct tttcaaaaaa ctttttataa ggacaagaaa gccatcgtaa
    10201 tcgaaggtac attagttcgc acttttagac attgtctatt ttgtaaagaa actgcgaagc
    10261 agctcgtgaa aaacggcaag aagatttcaa tgattatctt aaatcgcagt gggaataaat
    10321 aaacctattt gcgtcttaga aaacaacatt accactacca atcatgcgga aaatatttta
    10381 ctgctcatac gtatcttgtc gttccttcat gttttatttc gaaacaagtt cattacacga
    10441 tattagaaga gttgaccgag cgtcaagcgt taaagacact tacgaaacgt tgtgatcttt
    10501 cagtaacgac atttcaacgg acattatctg ctctatcggc aatgctgcga cagatgctag
    10561 actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt tcccatggaa
    10621 agttctcctt ttcttgtatg aatagagaga cagggaagct ctttgacatt ttgtccagcc
    10681 ggagaaaaaa agatttgatt gcttatttca tgcgttttac aagaaaagct catatggggg
    10741 ttcgatacat tgtcacaaat atgaatgccc cttatttttc attagtcaaa gagtcctttc
    10801 caaacgccca aatcatcatt gattgctttc acattggaca acacttgaac aagtttttcg
    10861 atcgtgttcg aaaacgtgtg atgaagcagt tgaatcaaaa agacacttca caagctaagt
    10921 actatcgaca attgaaacct ttgtataaat tacttctaaa atcagatgac gagttggatt
    10981 acacaacatt caagaaatgg cagaattttc aatggcgcta tttaacagaa agtgaagtag
    11041 ttgatcgtct accctcaatc tcttatgaac ttaaaattgc taagcagttt atcaaaagct
    11101 gcttagcaat tatcacaaga aacagctagc tacttcgaga aacgaccaaa gaagttccct
    11161 cgaaacttca ctatctcaaa aagccttttt catatctgaa aaaggctttt aactagcatt
    11221 ttcgaaaaag tattctaata acaaaattag agaatcttcg cacacatatc aaaacattga
    11281 aacgatttgc ttatgggttt cgcagtttta ccaatatgcg cttgtggatt tttctaatca
    11341 acaggttaat cagctatgca ttaaaagcta gtaactctta ctaactttgt tgttcataaa
    11401 tgaccttatc agcccgattt gacaaagaac tactttttta caacattatt ttattttcga
    11461 gtaatctttc ttatttcatt tcgtatatct ggaaaaagaa aataaacaaa cggaagataa
    11521 ataatgaaaa tcattattga tttaattgcc atatctaaaa atgaagaaga gaataattgt
    11581 ggaagaaagt aataggccca agtagtcaaa gtaagattga ctataaaaaa taagataacg
    11641 ttcttcccaa agaaatagga tatttttatt tcttttcgta caaaaaatac tctaaagaaa
    11701 aagactaata attctactac cacgagtacg atcgtagccg ctacagcgcc ataagtaggc
    11761 acaaaagcta catttccaat aatacttaca atcgcaccaa tgatcattgg ataagcatac
    11821 tccttatctt tttctatagc aattgcaaat tggttagcaa aaactccacc aattggacta
    11881 acaactataa ttagacaaga aataagcatg tttattgtca tcggtttgaa attctcccca
    11941 aaaaaccatg gaacaaattc ttttgtattg ataaacacca cactaaaaag aattaatgca
    12001 atgataatcg tgtaatcaaa agattttttt gtgtaaacta aaatattttc tgatttcccc
    12061 tctgcctgtg ctttagatat tttaggcaac ataacaatag taattgatgt taagactgcc
    12121 aatataatac gagcaatttt ttgagactga tcataataag ataattccgt tggatccaca
    12181 aaatacccaa ctatcgtttt gtctaaagtt gtgtacactt gcataaacaa ctgaggagtc
    12241 aaaataataa aagctggttt ccacaactgt ttaaaaacat tagagttaaa gattggttta
    12301 cctacttttc cgtggatact taaccagaaa actaaacttc ctaaaaagtt agtaattgaa
    12361 gtaatgaaca cgtaaatcca caaatcatct ttagttgaaa caaaaatgat tattaacaat
    12421 accgatgata atttgataac tgtatttcta ataaccactt tcgaaaattc ttcaattcct
    12481 tgaaaaaacc aagaaatatc taatgttagc cctacaataa atatcaattg aattaagtat
    12541 aaatttttat actttacatc aaaaaaataa atataggcta tatagagaaa ggaaacactc
    12601 aagcctatta aaaattgaat aaagtaaata ttccagaatt gtaaagatag tgctttttta
    12661 tctttccttg atttagcgat atttcgaata cgaaaaggat tcattcccac agcaatgaca
    12721 gtcattaaaa actgtgatat tgagaaaaca tacccataaa tccctaaacc agttgaacca
    12781 attctacgac taagtatagg tatagtaagt aagggtaaaa caatcaaaaa gacttgatat
    12841 atcgcattat aaaatatatc ttttactgtt tttttcactt tatatccctc tctatcaact
    12901 caagaacctt ttctgtactt gctcctgttt cataatttcc aatataatca agtaactctt
    12961 gagaactttt caaaagcttt tcttgttggt ttttaaaaac atcaacaagg ccataactat
    13021 cttgtacgct tataattggt aactcactta actgtatata aaaacctctt tctaagctat
    13081 attctttttc atcatagtta aaaagaataa ttggtttttt tgttagcgca aaatcgaaaa
    13141 aaatagatga ataatctgtg attaaaacat ctatctcttc aaaaagcatt tcaagaggaa
    13201 tcccatcatt aattaaagta atttgatgtt ttttcatata ttctatactt ttagaatcag
    13261 ttgctaattt actatgaaca ttaggatgaa atttagtcat aaaatttaat tcttgttgtt
    13321 gctttttaaa aaattcacgt aagttatcca cacctaacca ttctagttgc gtaattccgt
    13381 tgtcctcaat attatttcga aaagttggag caaataagac attgatttgt ttctttttca
    13441 agtcaaacac ttgctcttta ctagaggcta attcatcgtt tctaggagtt cctacttcta
    13501 atattttaga aggaacgata ttgaatgctg aaacatactt cttagaacta tagcgattac
    13561 cagataataa atatgtaatt gactcactat cttttttcgc atagcttagg taacgtttag
    13621 gtaacttatc ttttgcatca aattcaattt tttttaaagg cgttccgtgc catgtttgaa
    13681 aataaagttg ttttttcctt tttgcatact taatcgtttt ccgactatcg tctatccaat
    13741 acttagctgt atataaataa aaggcagcta aaaaagaatc ggagtcaatt ctt
//
LOCUS       DAFCLG010000065        13700 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFCLG010000065 DAFCLG010000000
VERSION     DAFCLG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.065"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..901)
                     /locus_tag="LND56_001759"
     CDS             complement(<1..901)
                     /locus_tag="LND56_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBK6743679.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="LND56_001760"
     CDS             1184..2152
                     /locus_tag="LND56_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6743680.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="LND56_001761"
     CDS             2265..2543
                     /locus_tag="LND56_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6743681.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="LND56_001762"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="LND56_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK6743682.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3000..3755
                     /locus_tag="LND56_001763"
     CDS             3000..3755
                     /locus_tag="LND56_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6743683.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="LND56_001764"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="LND56_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK6743684.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="LND56_001765"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="LND56_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK6743685.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="LND56_001766"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="LND56_001766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK6743686.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="LND56_001767"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="LND56_001767"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBK6743687.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="LND56_001768"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="LND56_001768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK6743688.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="LND56_001769"
     CDS             8492..9034
                     /locus_tag="LND56_001769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK6743689.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /locus_tag="LND56_001770"
     CDS             9057..10613
                     /locus_tag="LND56_001770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK6743690.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="LND56_001771"
     CDS             10627..11529
                     /locus_tag="LND56_001771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK6743691.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="LND56_001772"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="LND56_001772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK6743692.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="LND56_001773"
     CDS             12972..13394
                     /locus_tag="LND56_001773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK6743693.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2450 c         2988 g         3732 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatttggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctgcttcgc cgtccaccag
     9901 gtcgtgaagc ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgtctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgttgctca gccgtcctgt taagaaaacg ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttacaac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcgg
    13561 aggttgtgaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
    13681 aataagggac tgcgacaaga
//
LOCUS       DAFCLG010000066        13591 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFCLG010000066 DAFCLG010000000
VERSION     DAFCLG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.066"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..1760
                     /gene="yfcC"
                     /locus_tag="LND56_001774"
     CDS             21..1760
                     /gene="yfcC"
                     /locus_tag="LND56_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK6743694.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1917..3101
                     /gene="iadA"
                     /locus_tag="LND56_001775"
     CDS             1917..3101
                     /gene="iadA"
                     /locus_tag="LND56_001775"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK6743695.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3212..3415
                     /locus_tag="LND56_001776"
     CDS             3212..3415
                     /locus_tag="LND56_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6743696.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3516..4178)
                     /locus_tag="LND56_001777"
     CDS             complement(3516..4178)
                     /locus_tag="LND56_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743697.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4191..5693)
                     /locus_tag="LND56_001778"
     CDS             complement(4191..5693)
                     /locus_tag="LND56_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743698.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6241..7920
                     /locus_tag="LND56_001779"
     CDS             6241..7920
                     /locus_tag="LND56_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6743699.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8047..8526
                     /locus_tag="LND56_001780"
     CDS             8047..8526
                     /locus_tag="LND56_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBK6743700.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8745..9425
                     /locus_tag="LND56_001781"
     CDS             8745..9425
                     /locus_tag="LND56_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK6743701.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9433..10764
                     /locus_tag="LND56_001782"
     CDS             9433..10764
                     /locus_tag="LND56_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6743702.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10779..11741
                     /locus_tag="LND56_001783"
     CDS             10779..11741
                     /locus_tag="LND56_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBK6743703.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11852..12190
                     /locus_tag="LND56_001784"
     CDS             11852..12190
                     /locus_tag="LND56_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBK6743704.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12269..12976
                     /locus_tag="LND56_001785"
     CDS             12269..12976
                     /locus_tag="LND56_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBK6743705.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13079..>13591)
                     /locus_tag="LND56_001786"
     CDS             complement(13079..>13591)
                     /locus_tag="LND56_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBK6743706.1"
                     /translation="FYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLR
                     EKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQ
                     LFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMK
                     KGYENKTKRM"
BASE COUNT         4305 a         2401 c         2968 g         3917 t
ORIGIN      
        1 aaagaaagga agaaataaat ttgaagaaaa caaattcttc aggaaaaccg aaacgcgact
       61 tcactaaatt atcgacaccc catacgtatg tcattatttt tggtgtagtc atctttgcat
      121 ggatactgac ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt gaatacaaag
      181 atgcaaatgg cgaaacaagt actcgtactg ttttacgtca agattccttc cgttatgctt
      241 acgagctgga taagtcgtat gtgtttgatc aattagaaga actgcaagat catccagcag
      301 aacgtgaaaa actggatgtt ccggaaaaag gattggaaaa agtcattgct gacggagaaa
      361 aaaatcttac gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg ctttacgatc
      421 agtacggcga aaatatctat gatacttcta aaaaattgca taaaacagcc aaaatatggg
      481 gaacagatga ttttggcggc tttggtttct taaattttgt ttttgaagga ctagtatctg
      541 gagataagta tggatctgct gtagggatcg ctgcattgat ccttgttgtt ggaggtgctt
      601 ttggtattat tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt atcagtaaaa
      661 ctaagggact ggagcgttta gctttaccgc tattattctt tgctttttca ttcggtggcg
      721 ctacattcgg gatggcagaa gaggtaattc cattctcaat ggtgatggtt ccatttgtca
      781 ttgcgttagg atatgactcg atcgtagcag taaccgtgac ttatgtcgca tctcaagtcg
      841 gaaatgcgac ctcttggatg agcccgttta gcgtagcggt ggctcaaggg attgctggaa
      901 tccccgtgtt gtctggtgcg acattccgat tgattatgtg ggtagttgtt actgcattag
      961 ccgctggtta tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa agttcgttga
     1021 cttacaaatc agatgattat ttccgttctc atatcaaaaa gacatctgat gaaaataaaa
     1081 aattcatgct tgggcataaa ttgatcttat tagagatgtt agttgtcctt gtctggatcg
     1141 tttggggcgt gactcaaaaa ggctattaca ttccagaaat cgcttctcaa ttctttgtga
     1201 tgggattagt tgctggaatc attgctgttc ttttcaaatt agatggaatg aaagtcaatg
     1261 acatcgcttc ttctttccaa tctggtgcag ctgatttagc tggtacagca atcgtagtag
     1321 gtatggccaa agggattctt cttgtcttgg gaggatctga tgcatctgtt ccttcagctt
     1381 tgaatacgat tttgcatggg atcgggacgg cgttgaccgg tgttccagct gtcattggtg
     1441 cttgggcgat gtacatcttc caaagtttgt tcaacttggt tgttacatca aattcaggac
     1501 aagcagcttt gactatgcca atcatggctc cactggcaga tttagtaggt gtatccagac
     1561 aagtagcagt tcttgcttat cagcttggtt caggttttat ggacgctttt acacccgtat
     1621 cagccagctt gattggtgtg ctaggtgttg cacggatcga atgggcaaaa tgggcaagat
     1681 tccaaatcaa gatgcaagga ttcctgtttg ttttaggaac tattttcatc atgattgcca
     1741 ttgcaatagg tctgcagtag tactggagac ctgtaaataa aaaagtaatc caaacagtag
     1801 ataaggactc caagaaccgt cattttacgg taaaatcaaa ggagtaaaga tcctatcgtt
     1861 tggattattt tttaagatcc tatcatcagt tatacttaaa gaaagaggaa aaatgtatga
     1921 aaatcattcg acaaattgaa gtgtttgcac cagattactt aggaaaaatg gatgtactga
     1981 ttgccggtgg gaagattttg gctgtagaag aacagctaga aggcggctac gaaggagtgg
     2041 aagtagaaga actttcaggt gagggaaaag tactaacccc gggtttcatt gactgtcatt
     2101 tccatatttt aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca gaagtcacat
     2161 tgagccaatt gactacagct ggtgtaacga cagttgtcgg ttgtttggga acagacggag
     2221 aaggtagaga tatgactgcg ttgatcagca aagcgaaagg tctggaagca gaaggaatct
     2281 ccacttatgt atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca gattctatca
     2341 tcaaagactt tttgacaatt gataaaatta tcggaatcgg agaaatcgca gtatccgatc
     2401 accgttcttc tcagccaagt tttgaagagt tcgctcgtgc agtagcagat gctcgtgtag
     2461 gaggcatgtt atccggaaaa gctgggatca tcaatgtcca cttaggcgga ggaaaaagaa
     2521 agctagagct attgacacga atcgtagaag aaacagaaat tccgatcaca caatttttgc
     2581 ctactcatgc aaatcgcaca ccagaattat ttgaagcttg cgtggcattt gccaaacgtg
     2641 gtggcacgat tgacttcact gctagcgaag atcctgattt ttgggagaag acagatggtg
     2701 aagttcgctt cagcaaagca ctgaaacgat tgatcgagga agatgtttct ttagataatt
     2761 tcacgatgac ctcagatgga caaggaagct tgccttattt tgatgaaaac aatcatttcc
     2821 tagggttagg agtaggaagt gccaaggcat tgctagtagg gatcaaagaa gctgtgcaaa
     2881 aagaaagtat ccctttggaa atcgccttac gagctataac cagtaatcca gcacgtatat
     2941 tgaaattgga taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg tgtatcttgg
     3001 ataaagaaac gcttgatatt gatacagtta tcgctaaagg agaaataatg gtccaagaaa
     3061 aagaagtcaa agtatggggg actttcgaaa aaagttttta atcaacagct ctaactatct
     3121 tttttagaaa acgagcgttc tgtttgtctg tatctgttgt tgatgttacc ctgaaggtag
     3181 atcaataaac aaactaagag gaggaagcac aatggcagat ttaaaaggac gttctaaaga
     3241 tatgaaagat aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa cagacgacaa
     3301 gagcaaagaa acagaaggga aagcacaatc actaggtgct gacatcaaag aaaaagctcg
     3361 aaatttaggc gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa aataaaaacc
     3421 acttattact aattgaatca agcaaataaa aaatccgaac catatctgcg agacagatat
     3481 ggttcggatt ttttatttga tcattatcct gttttttaaa cttcgatttt ttgaaatttt
     3541 ttatttttta gttcaaagac ttcatcacag ctttttgcta cttcaatctc atgagtcacg
     3601 atgataatac agcgattttg ttcatgggcg atttcttgga aaagtttgac gatatcttgt
     3661 gaagttgttt catctagatt tccagtaggc tcatccgcta cgattagttg atgttcacag
     3721 cagatagcac gaacgatagc gactcgctgc tgctgacctc ccgaaagttt ggtcacctgt
     3781 ttttttgcta atatttcatc aattcctaca cgagccagtc gatctaaagc aacttgtcga
     3841 taattctctt gcttggtctt agtgatttcc attgctgcta tgacgttgtc tagagccgat
     3901 aaataaggaa gcaagttgta agcttggaaa ataatcgaga catcattttt acgataactt
     3961 cttaacccga ttttgttcag tgaacgatct tgatagtata cctttccttc ttttggtgca
     4021 tctagtccag aaataagcga aagaaaagtc gtttttccag aaccacttgc tcctaaaata
     4081 gcgtacattt ttccagcttc gaaattcagt tctacatctt tataaagcgc atcttgcgga
     4141 tcttgatacc aaaatcccaa ttgttctgtc cgtaacatca aattcctcct ttagtttaat
     4201 aggatttttc ttgggtttaa tcttaatatc cctatagaag ctaaaaagac agaaaacaga
     4261 ctgattccaa atgctactcc ggctaatagt cccaattgtg caggtttgac attgatttcc
     4321 aattcgctga cttgatcaga aacagcaaat ggattattcg tggttccttg ttgaccatcg
     4381 cgttgaccgc cttgttcttg tctgttacct ggcatttgtc caccgccagg agcttgctgg
     4441 ttttgtgtcg tggtttctgt ctgttggtca agtagctgct gtcccacggc attcgcgacg
     4501 acgtttccgc taagcgaagc aactcctaat gcaagaatca tacaaatagc cacttcggta
     4561 aacaattgga gaatgatttt acttctggat tctcctaaag atagcaagac gcctaactca
     4621 tgccgacgct cccgaatggt aatcataatg atcagagtaa gtatgataat accggctaca
     4681 gccactagta aaacaacatt tttagaaaaa cttgccacgt tgtttaaagg ttccaacatt
     4741 gactgataca tggaatcatt tgattgtaag ctaaatgtat ctgtgtcgat cagcttctca
     4801 gcttctttga gaaactcatc cattttatct ggatcagata gagtgtaaat agcagaatct
     4861 atcgtgttat cgtcagatgt cccatttagt tcatttgcaa aagtgtaaga agtgtaaagt
     4921 gtattcgacg gattcatgaa gttgaatttc atacccatac tgccgcttgt ttcgctggat
     4981 tcataaattc ctttgatcgt catttcataa gtggtatctt catcttcagt actcgtgatt
     5041 gtgaaagtat ctccgactgc caggtcattt gtttctgcta gagtagaatc aatgactaca
     5101 ttgtttgtgc ctttgtcgct gtcatctatt ccttcaccat ccgtgatttt tgctgttcca
     5161 tcagaaaaat tactattttg tgcagttgct gacacaccac tgatttgaaa gtctgcttga
     5221 gtcatttgcg gattcccgcc cattcctcca gcaggttcac cgcctgcatc agtagtttct
     5281 gaactgtctt cacttgaatc agaactagtg atggcagtta ttccatcttt tgccatagct
     5341 gaggcggaac tttcaaagga ataactcttt acattatcta attcagctat tttttccgcg
     5401 tccgttaagg aaactggtgt aagagaaaaa ctgcctggat ctggacgttc ttcagtggta
     5461 gaaccgcttg aagaagtttg ttcctgtttt ttaaatgaag cttctcgatt ggcacttaaa
     5521 gtcactgttg ctccaacttc tttttgtgct tgattcgctg cttcttctgc agcgcttcga
     5581 atcgtgaggc cagctagcac aaaaataaga atggctgtga acacggcaaa caataacaat
     5641 gaacgtcccc attttacttt tgtacttttc catgcacgtt tcataaaatt catattttgc
     5701 atccctccta gtagatgcat ttgataatag acaacctatc ttaaagctag cttaaatctt
     5761 atgctgtttg cacaccatca acatgaggtt ctgtatatat aaaaagtcac tatagttatt
     5821 gcaaaaaatg tggtaaaatg aaaaaagtat gtttcaaacc gacgaagaag aaatgaaaga
     5881 ggaatagcaa aagaaatcct agatgctaca gagggatttt tacgtgcata gttgttctaa
     5941 caattgctga tactagcgat ttagtttatt tgttgtataa taatggggaa cagctgaata
     6001 cttatataag tattctggct atagcgtttc gcaaaagatc cattttgtta atgaagtata
     6061 tccaaaataa aatgcccatc caactgtcag aacgcggatg tgctggtaat tttcctgctg
     6121 attctccata agttgagaaa tagtgatttt gcatatgctt cattggaatg gctcatatat
     6181 ttagataaag aagaaaatcg acatctttaa acttggatgt cataagaaga ggtgaagttt
     6241 gtgagtacca ttaaaattat cccattaggc ggcgttcgtg aaaatggaaa aaacatgtat
     6301 gtcgcagaag tgggcgagga tatttttatt ttagattgtg ggctaaaata tcctgaaaat
     6361 gagttgttgg ggatcgacgt cgtaatccct gattttacgt atttagaaga aaacattgat
     6421 cgtgtagctg gtgtcttttt aacacatgga catgcagatg cgatcggggc acttccttac
     6481 ttgttgtcaa aaatttctgt accagtattc gggacagagt tgacgatcga gttagcaaaa
     6541 ttgaatgtca gtcgtaatga agcagccaaa ggatttaagg atttccatgt ggtagatgag
     6601 catacagaaa tagactttgg tgaaacagta gtgagctttt tccgtaccac tcatacgata
     6661 ccagattcag tggggatcaa tctgaaaaca aaagaaggta gtatcgttta tacgggagat
     6721 tttaaatttg accaaacggc tatcccaatg tatcaaacag attatgcacg attggcggaa
     6781 atcggcaaag aaggagtctt agccttacta agtgaatcgg caaatgcaga aaatcctcag
     6841 cctgtagctt ctgaagccca aatagcagat gaagtatttg atacgatcaa atattgggaa
     6901 gggcgtatca tcgtcgcttg tgtagcaagt aatttgcaac gagtgcaaca agtattggat
     6961 gcagcagaaa aagcacaacg aaaagttgtc ttgactggtc aagatttcga acgaattatt
     7021 cgcacagcga tgcgtttaga aaaacttcaa ttaccaagtg aagatctgct tgttacgttg
     7081 aaagacatga aaaactatga tccagaagaa ctgatcattt tagaaactgg ccgaatgggt
     7141 gaacccatca agtctctgca aaaaatggca aacggtacac accgtaatgt caagattgaa
     7201 gaaggagatt tggtttatat cactactacg ccaagcatcg cgatggaaac catcgtggcg
     7261 aaaacggaag atatcattta tcgagctggt ggaacagtga agttgatctc tgagaatatg
     7321 cgcgtttctg gccatgccaa tcctaatgac ttgcaattaa tgatcaattt gatgaaacca
     7381 acatattttg tgccagttca aggagagtat cgtgaattag ctgctcatgc agatttggct
     7441 catgcagtag ggatgccata caaaaatatc tatattactg gacgcggaga cgtgctggaa
     7501 tataagaaca agcgtatgag tgttgcagga actacaagtg cagaaaatgt aatgattgat
     7561 ggattaggca taggtgatat cggaaacatt gttttacgtg atcgaaaaat cttgtcagaa
     7621 gacgggattt tcgtagcggt tgtcactatc agtcggcgag aaaagaaaat catttcaaaa
     7681 cctcaaatta cttcacgggg attcgtttac gtaaaagcta gccgtgatct gatgaaagaa
     7741 agcagcaata tgattgaaac aatcgttgag aaacatttag aaagcaatga ttttgagtgg
     7801 agcaaattaa agcaagacat ccgagatcaa ttaggccgtt acttatttga acaaacaaaa
     7861 cgccgcccag tgatcttgcc agtcattatg gaagctactc aacgtcgaaa taaaaaataa
     7921 acaaaaagag taagatgaac ctctctatag agtgctcgtc ttgctctttt tttgcgaaaa
     7981 acaaataatt tttgaataca gcatccctcg ctttataata aacgatagaa taaagggagt
     8041 gaatcaatga tgagtcaaac aaaagtcgaa cgttatttag aagaacaaaa gatcagctat
     8101 caaccacttg attttagtca tgtggtagaa gaatctttca cagaagaact acaaaaaaga
     8161 gggattgatc cagctttgat atgtaaaact ctcgtagtga aaggaaacaa aactggggtg
     8221 atcatcgcat tgattccttt gaacgatcac ttagattaca aaaaaacgag acaagtgacg
     8281 aaagatcgta aagttggttt tcctggaatg gattttgtat taatgcatac aggatatccc
     8341 cacggagcaa acacccctat tggaataaaa ctcgcacatc ctgattattg ttttttagca
     8401 gacagttctt taaaagaaaa agaagaagta attgtgtcaa gcggtgaaat cgggagaagc
     8461 cttcggataa aagtagccga tttagaaaag atcgtccatc ctatttacgc tgatttaatt
     8521 agttaaggga cgagaagaga agctaagcag agcagagacc tcaaacttga gattatatct
     8581 gcttggtact tggtgcatcc tgtattatct gttacaattg ctttgtacat actgtactat
     8641 caatccaatg aaaatatatt tagcagctat attttttcat tgatgtgcaa gtgatgaaat
     8701 cgtttttaat aaaaaagata cagagaaaac gcgaggtgta aaatatgaaa attacgtatc
     8761 atggacattc ctgtttaaca attgaaatgg aaaatggcca acgcgtgatg attgacccgt
     8821 ttattacagg aaatccattg acagacttaa ctgcagaaga agtaaagaca gattggttgc
     8881 tgattactca tggtcatagt gaccacatag gagatatgat tcctattgca aaagaaaatc
     8941 aagccacaat tatcagtatt gttgagattg ctgattatgc gaacagcaga ggagtagatt
     9001 cattcggtat gaatatcggt ggaaaacatg cgtttccatt cggcgtggta aaattcgtac
     9061 atgcacagca tagttcaagc tatgaagtag acggtatcgt tcagtatatg ggcgagccta
     9121 gcggaattat tattcaagca gaaggaaaaa cgatttatca tgcaggggat actgcttatt
     9181 tcagtgatct aggattgctt gcagaagaat tcgatattga tgtcgctttc ttgccgattg
     9241 gcgataacta tacgatgggg ccagaagatg cagcaaaggc tgcgcaaaca attcgtgcca
     9301 agaaagtagt tccaatccat tacaatactt tccctgtcat tgaacaagat cccgaatctt
     9361 ttgtagagtt actggaagat agtgaaggaa aaataatgag cgtctcggaa acgctggaag
     9421 tgtaggcaga aaatggcgac aaaacatgat ttgattttag aatatattga aagtttacct
     9481 gttggcaatc gaatttctgt aagaagcatc gcaaaagaat tgaatgtcag tgaaggaaca
     9541 gcgtaccgtg caatcaaaga tgcagaaaat gtcggactag tttcaacaat tcaacgagta
     9601 gggaccatcc gtatcgaacg caaattaaaa aaacatattg aacgtttgac atttggcgag
     9661 gtcgtcagga tcattgaagg ggacgtttta ggcggggcgg caggattaga caaggtcttg
     9721 aataaattcg tcatcggcgc gatgactaaa catgccatgg aacgctatat tacgccagga
     9781 tcactaatga tcgtcggcaa ccgggcggaa gtccaaaaat tagcattgga agatggcgcc
     9841 gcagtattga tcacaggagg attcaatacg actgctgaga ttgcaaagct agctgatgat
     9901 cttgaaatgc cgatcttacg cacctcttat gatacattta cagtcgcaac catgatcaac
     9961 cgagcgttaa gcgaccaatt gatcaaaaaa gatatcatgc ttgtcagtga tatctatatg
    10021 ccagctgaaa agactcatta tttgcagcag acagatacga tttctgatta tcagcgtttg
    10081 tcagaagaaa cgcagcattc gcgttttcca gtcgtcaacc gccatcatcg tttgatgggg
    10141 attgttacgg caaaagatgt tttagggaaa tcgccaaacc agatcatcga tcgggtgatg
    10201 acgaaagaac caatcagtgt aaagaaaaca atgagtattg cctctgttag ccatcaaatg
    10261 atctgggatg ggctagaagt catgccggtc gtttccgatg acttgacatt ggaaggcttg
    10321 attacccggc aagatgtaat gaaagcgatg cagcttgtcc agcgacaacc gcagattgca
    10381 gatacgattt cggatcagat atctggaaat atccatacga ttgatactga ccgtgaagga
    10441 aatcgtttgg aaaaccctaa gttcaaattc gtagtagcac cacaaatggt caatggcgtt
    10501 ggaaccattt cttttggcgt acttagcgaa atcatttctg atgtcgcaca aaaaacaatg
    10561 gtgatgaacc aaaaacgaaa tatcttgatt gaacaagtga acctacatta tttgcggctg
    10621 atccaattgg aaagcgaatt agacattcgt ccacgtgtat tggaaatcgg cagacgttcc
    10681 gctaaactgg atatcgaagt gtttattgaa aacaccatcg tttccaaagc aatcgttgtt
    10741 tgtcaagtaa tggaacgttc ttagaaagca ggagtgaaat gacagtacag caagaaatag
    10801 tagatacaat aaaaaaatac gataaaatca ttatccacag gcatttgcgc cctgatcctg
    10861 atgcattagg atcacaagta ggattagccg aactattacg ggaaagtttc ccagaaaaag
    10921 aaatacgtca agtaggtgaa acgattgaag ggcttcgctt tttagcagaa atgcaagaag
    10981 ttgaagatgt cttctaccaa ggagcgctag tcatcgtgac tgatacagct aattcaccca
    11041 gaatcagtga tcaaagatat cagttggcag aaaaactgat caaaatcgac catcatccca
    11101 atgatgaccc ttatggagat cttgtgtggg taaatacaaa agcaagtagc tgtagtgaga
    11161 tgattgcaga atttgctttg atgtttcctg aatctttaaa gatgaacgtt aaggctgcac
    11221 ggttacttta tgcaggaatc gtcggagata cagggcgctt tttgtatccc tccacgacgt
    11281 cccgtacatt agagattgct tcgatattga gaaattatcc atttgatgca agtgctttga
    11341 acagagagat tgaacaaatg ccgatgaaag tcgctaaact ttccggttat ctttatcaaa
    11401 atatccaggt ggatgaaaat ggtgcaggaa gaataatcct tccgcaagaa attcttgata
    11461 aatatggaat cgatgattca gagacagctt cgatcgtttc acttcctgga gtgattgacg
    11521 aagtgctggc ttgggggatt tttgttcagc agccttcagg gtattaccgc gtacgtttac
    11581 gttctaaggg gccgatcatc aatgaattgg ctaaacgcca tcatggagga ggccatccat
    11641 tagcaagtgg tgcgaatgca aaagatttag cagaagtaga ggaaatctat aaagagatcc
    11701 aagatatatg ccaatcgtat aagagcagaa aaacagaatg atacttcgtt cgaaaacgtg
    11761 ccttttgtag taaaaatcac aaatgtatct tttcaagatc ttctttttcc gttacaatag
    11821 tgctcactaa agattttaaa ggagaaagaa gatgacatta ccaaattgcc cagaatgtgg
    11881 ctcagagtac acatatgaag atcgaggctt gttgatttgc ccagaatgcg ggaacgaatg
    11941 gaacaaagaa gaacaagaaa ctgtcataga agacggatta gtggtattag atgcaaatgg
    12001 aaaccgtctg ttagagggcg atagcgtgac agtgattaaa gacttgaaag taaaaggtgc
    12061 gtcaggtgcc attaaacaag gaacaaaagt caagaacatc cgtttagtag aaggcgatca
    12121 taatatcgat tgcaaggtag aaggatttgg accgatgaaa ctgaaatcgg aatttgtgaa
    12181 aaagaattaa agaatattca aggtcttttc acattctttt cctactacta atgataaaat
    12241 aaagtaaacg aaaaggagtg aaacgaaaat gtatggttat ggaatgtatg gttttttaga
    12301 tccgacattt cttttggtca ttcttggatt agttatttcc ggaattgcgt cagcgtacgt
    12361 caatagtact ttccgaaaat acgatcaagt gagaagcaaa aacaatgtga ctggtacaca
    12421 agcagcacaa tatatattac aaagtcaaca gataaataat gtcggtgttc aacaaattgc
    12481 cggagatctc acagataact acaattcagg taataagatg ttgagtttgt ctcaagcaac
    12541 tgcacagtct acttcagttg ctgcgatagg tgtagcagct catgaatgcg gacatgcggt
    12601 acaagatgct tcgggttatg tcccattgaa gattcgtgca gcacttgttc cagcagctaa
    12661 ctttggttcg atgatttcgt tccccttgat tatggtaggt gtattgctta gctggaactc
    12721 tacgttaatc aatatcggga ttttcgcttt ttctttagca ttacttttcc aattggtgac
    12781 attaccagtc gaatttaacg cgtctcggcg agcaatccaa attttgagtg aaggcggact
    12841 actgacggaa gaagaagtac cgatggcaag acacgtatta tttgcagcag cactgactta
    12901 tgttgcagca gcgctttcga cattcctgca gctgttgcgc ttgatcttat tgttcggcgg
    12961 caaccgaaga gattaaaata aagaagttgt gaaatcagcg ctttgatctg agctaatacc
    13021 gagagttagc tgattgaata ccatttattt ggaataagag gatgtgacaa gaattttgtc
    13081 acatcctctt tgttttgttc tcataacctt ttttcatata cagttcatga accgctttta
    13141 ttaacttgcc aaataacact gtattgtgcg ttgtatccag atagaaagtc tccgtttcat
    13201 ctgaaatttt ctcatgctga tctgtaatga tcaaatcagc atgttctgaa tatgcggtaa
    13261 ttttaacagc atctttgcga aataactgct gcaaatggct ttgaagatga taatttcctg
    13321 taaactgttt ggttatctgc aaatggatga gtaactgctc tttcgtttct gtttgtagta
    13381 agtgttggat caattgacta aggtattgac tgatttggtt tctttcccca ttttttgaaa
    13441 aaattgtatc gattttttct cttaatttct tttcctgtat aaaagaacta gaatgattgg
    13501 acgtggcttc tggaaagcaa agtgtagaaa aactactgct ggattcaccc aacagttcga
    13561 aaaaaacaac tgccaacaca agaaaataaa a
//
LOCUS       DAFCLG010000067        13418 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFCLG010000067 DAFCLG010000000
VERSION     DAFCLG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.067"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="LND56_001787"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="LND56_001787"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK6743707.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="LND56_001788"
     CDS             1011..2258
                     /locus_tag="LND56_001788"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK6743708.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="LND56_001789"
     CDS             2334..2678
                     /locus_tag="LND56_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743709.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="LND56_001790"
     CDS             complement(2795..3121)
                     /locus_tag="LND56_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743710.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4695)
                     /locus_tag="LND56_001791"
                     /pseudo
     CDS             complement(3494..4695)
                     /locus_tag="LND56_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4812..5327
                     /locus_tag="LND56_001792"
     CDS             4812..5327
                     /locus_tag="LND56_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6743711.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="LND56_001793"
     CDS             complement(5526..5972)
                     /locus_tag="LND56_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6743712.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="LND56_001794"
     CDS             complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="LND56_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6743713.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="LND56_001795"
     CDS             complement(7851..8480)
                     /locus_tag="LND56_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6743714.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="LND56_001796"
     CDS             complement(8464..8928)
                     /locus_tag="LND56_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6743715.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="LND56_001797"
     CDS             complement(9415..10683)
                     /locus_tag="LND56_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6743716.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="LND56_001798"
     CDS             complement(10914..11651)
                     /locus_tag="LND56_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6743717.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="LND56_001799"
     CDS             complement(11718..12287)
                     /locus_tag="LND56_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK6743718.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="LND56_001800"
     CDS             12415..13287
                     /locus_tag="LND56_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6743719.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         4046 a         2759 c         2429 g         4184 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactat
//
LOCUS       DAFCLG010000068        13374 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFCLG010000068 DAFCLG010000000
VERSION     DAFCLG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.068"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..576)
                     /locus_tag="LND56_001801"
     CDS             complement(34..576)
                     /locus_tag="LND56_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBK6743720.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            711..1100
                     /locus_tag="LND56_001802"
     CDS             711..1100
                     /locus_tag="LND56_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743721.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1104..1868
                     /locus_tag="LND56_001803"
     CDS             1104..1868
                     /locus_tag="LND56_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6743722.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1997..2944
                     /locus_tag="LND56_001804"
     CDS             1997..2944
                     /locus_tag="LND56_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6743723.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3074..3814
                     /locus_tag="LND56_001805"
     CDS             3074..3814
                     /locus_tag="LND56_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743724.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4105..4773)
                     /locus_tag="LND56_001806"
     CDS             complement(4105..4773)
                     /locus_tag="LND56_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6743725.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4907..5194
                     /locus_tag="LND56_001807"
     CDS             4907..5194
                     /locus_tag="LND56_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743726.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5610..7148
                     /locus_tag="LND56_001808"
     CDS             5610..7148
                     /locus_tag="LND56_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBK6743727.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7145..8110
                     /locus_tag="LND56_001809"
     CDS             7145..8110
                     /locus_tag="LND56_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743728.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8097..9419
                     /locus_tag="LND56_001810"
     CDS             8097..9419
                     /locus_tag="LND56_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6743729.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9482..9586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9660..10403
                     /locus_tag="LND56_001811"
     CDS             9660..10403
                     /locus_tag="LND56_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743730.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10418..11410
                     /locus_tag="LND56_001812"
     CDS             10418..11410
                     /locus_tag="LND56_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6743731.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11422..12159
                     /locus_tag="LND56_001813"
     CDS             11422..12159
                     /locus_tag="LND56_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743732.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12149..12871
                     /locus_tag="LND56_001814"
     CDS             12149..12871
                     /locus_tag="LND56_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6743733.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12955..>13374)
                     /locus_tag="LND56_001815"
     CDS             complement(12955..>13374)
                     /locus_tag="LND56_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK6743734.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4400 a         2291 c         2785 g         3898 t
ORIGIN      
        1 atttagatat tagacagttt ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt
       61 tttagtccag aaaccacctg aaatatattt tggctcataa gaaatgataa aagcacgctc
      121 ttcgatttct ttgatcaggc gatacagttc acgttcgctt tttctaggag aaagtatttc
      181 gagaactagc cgctcacctt ccagccccat tgcagaagtc tgtgtcacac cataaccgtg
      241 ttctctcagt atacccggca aacttttatc ttctgcgcta tttgctggca aaatcgttgt
      301 gaccatgatg tatcctaatg ccaatttgtc ttcaatcaaa attccgacac tgatacctac
      361 tgcgtaacct aacgcataga caaataaatt cagtggatta tctaaacgat ctaaaacaag
      421 ggataagccg acgatataaa tcgtgatctc gaccatactg acaagtggag caatcagtcg
      481 atagcctttc attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag caaaattaat
      541 gaggaagatc atccccaaca tttttggatc aatcatttgt aaaactcctt tcatttatat
      601 aacaacaata tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata catacaaaaa
      661 aatgataaaa tgattataat agaattataa agataggtta ggtgattaaa atgaaaacgg
      721 tcaatcgtgt gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg
      781 tctattcgat tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag
      841 atggtttaat tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta
      901 gcgttgccag ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg
      961 tgggactttg ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg
     1021 gtacactgaa cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt
     1081 taatgaaaat aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata
     1141 ttccagtgtt agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct
     1201 attatcacgg atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc
     1261 agcagggcat tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg
     1321 tttcttctat tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg
     1381 acacacttat caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg
     1441 gggtttcgat ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag
     1501 cgggcgtgtg tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc
     1561 gcgcatcgga atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta
     1621 agagctatga tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc
     1681 atggacaagc tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc
     1741 ggcataagtc ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg
     1801 tacaaatacc actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt
     1861 ataaataatt gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg
     1921 ggctataata aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt
     1981 tagaaaggaa gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa
     2041 aatgacggtc tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt
     2101 agttttctca gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt
     2161 cacgaatcgg tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc
     2221 ctattacatg aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct
     2281 ccaattagtt acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg
     2341 tgtcagaaaa aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg
     2401 ggaaaattct gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc
     2461 aacgcaatat gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga
     2521 cgagcctttt gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct
     2581 tcctagtaaa atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga
     2641 aaactgggca gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact
     2701 aggagcagta agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat
     2761 tggatttgat ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca
     2821 agatattata cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa
     2881 aggggcgagt caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa
     2941 ttaagtctta ttactatata tatatttatg aaattcatcg ctattttctt aaaataatca
     3001 gcgcattgct actgaaaaca atgaatctgt gctattattg agaaaagttc catttttgag
     3061 aaggatggtt tgaatgaaag aaatcagacg aaaacatttc cgttttcctg cgtacgatga
     3121 tgagttaggc gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg gacaagaaga
     3181 tcttttgact gaacagaatc agtccccgtt tgaacagatg aaagaatttc atttcacgaa
     3241 cacgaacacc gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc
     3301 gcgtaaggaa aatttggaaa agcataaagg aaaattaccg gattataccg gccattacca
     3361 atcagaaatc acatcgacag gacgaaaaaa actttttggt gattcctctc gttctgtcag
     3421 acaaagtgaa acaaggacag agtccaagca tactacacga aacgcatctg ttgacacgaa
     3481 ggtgcgggag cgttcttatt ttgttcctaa atatgtaccc gcatcgatta tcccagaccc
     3541 tagagaaagc aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa cggatgactc
     3601 ttatctgttg ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc
     3661 tgtacgaaca ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa
     3721 atcgaaaaaa agccatggta tcttagaacg ctcattacaa ggattgatcg atgatcaagg
     3781 aaactctttg ggagaaaata gctattttaa ataagtgata tacaaattta tattcggcta
     3841 cagttggaaa ggaaacgctg cggcgtttcc ttattttata ctctatccga aagtttatat
     3901 cgtatcctca aatttttttg agtttaagcg aattttttct cccaataagt aagaaaataa
     3961 tggcaaaata aaaaaacaaa ctattttcgc agatcgtttg caaaaatagt ttattttttt
     4021 ataaagaaga ttttactgaa taatatcatt ttagtttaca taatgatatt tctgtaaatt
     4081 aaataaagta gtgaaaaata agaatcagtg ctctaactgt tcaagaggat caagtggttc
     4141 aaatgtttct gtagtctgtt ttgcttccaa ttggggcaaa acagatccaa tttccagata
     4201 cttcacttta ttcaatgtat aagatagaat agaacagcta agtaatgtat acagaaaatg
     4261 tgtgagatcc aaataaagca aggagacaac taaaatacca aaagtaatca agaaaagact
     4321 tttgccaatc ttaatatgaa aatatttttc aatggcttga taaatgatat ctgttccgcc
     4381 tgtgcttccg ccgaatcgaa agacgagacc gcaaccaatg ccagaaagaa tacctgtcat
     4441 caaactattg atcagtagac tatctacagc aaaatgtata tgaaggctct cccataaatg
     4501 taaaaagaaa actagcgcaa atgttccgac gatcgtccgg agaaaagcgt gttttccaaa
     4561 aagaaacaat gagataagca ataaaggtaa attaaaaagc agtgaagtga cagaaggagc
     4621 gaatccaaaa agattatgca aaagcaaagc aataccagta accccaccat ctgccagctt
     4681 agatggaata ttgattcctg tcacagctaa tccgtagatt cctgcgccta aaatgattcc
     4741 gcacaattct gtaaataaat ttaatttttt catttctagc ctcttttctt ttgaaaattt
     4801 tcagcaactt ctttattata ctgacttata ctcttacttc aagaggaaaa tgaaaaaaaa
     4861 ttatttttgg ctgtttcttt ttatttatca aataaggagt ggaaaaatgc gagcagcaga
     4921 cttattgaca attcttacaa aatcacccaa aaaaacagag tatcaaggag tttttttggc
     4981 tcaagaacaa aaactggtgc ctgtgacaat ggtccagata aataaagcaa atcaatttat
     5041 ctttttattt gaacacggca aagcaccact tgctatgaaa gacatctttc tttttttgat
     5101 gaagaaccgt gagaaagctg tttattatca gtcaaaagaa gaactcattc ctctttatgg
     5161 agtgaaagag atagaaaata aagtggtgat ataagtaaaa ataagaaaac aagacgttta
     5221 tgatgtcaaa acaaccattt tttacatttt tggcaaaaaa gtgtcatgat acagtataat
     5281 attcgttgaa caggccaata aaggtgttct cccccacgag atttctactt tattggctgt
     5341 ttctttttat taaaataaaa gttgtgtgtg taaaaaagat atagctgatt ccaagtgaca
     5401 atttcgtata gatttttatg gtattcgtag tgaaaaaacg agaaccaaaa agttgatccg
     5461 actttgttgc ttggattctt tttttgtcag catatttaga ggtatttttt tctttttttt
     5521 gttcgtgctg taaggttaag gcaagacaag ttagttggga aaaaggaaag aaatgtttat
     5581 acttaaagaa taacagaaag gaaggtcgca tgaataattt tatacaaaca ttaagtgaaa
     5641 gaaaaggcga gttgctggaa gctgcatttc aacatctgtc catttctctc atttctttgc
     5701 tgattgcagc actgattgct attcctttag cgatttgggc agccaatcat aaaaaaatgg
     5761 cagaagtctt attgcagatc acgggtgtgc tccaaacgat tccttcactt gccttattag
     5821 gtttacttat cccatttgta gggatcggaa ctgttccagc attgattgca cttgttattt
     5881 acgctttgct tccgattttc caaaacacgt atattggtct agctgaaatt gacccgtcta
     5941 ttgaagaagc agcggtagca tttggtatgt ccagaatgag acgcttgctg aaagtagagc
     6001 ttcccatcgc attacctgtc attatctcag gtatccgaac tgccttagtt ttaatcatcg
     6061 gtacggctac tttggcagca ttgatcggag caggtggatt agggacattt attttgttag
     6121 gtattgaccg aaatacacct gttttgacat tgattggtgc gatcagttct gccttgcttg
     6181 caattatatt cagcggactg attcgctggt tgcagcataa gaaacctcga tattccatca
     6241 ttactttagc agcagtggtg tgtttatttg gcggcgtttc tttatatcag tctgccttgt
     6301 ttaaaaatga aaccatcacg attgctggaa aattaggagc ggaacctgaa atcctgatcc
     6361 aaatgtataa agaattgatt gaagacaaca caaaaacaga agttgaatta aaaccgaatt
     6421 ttggcaagac gagttttttg ttcaatgcac tggatcaagg tcaaatcgat atctatcctg
     6481 agttcacagg tactgtccta gaaagcttgg tgaaggtacc tgaagcaatg aaaagtgctg
     6541 atttgacaaa agaaaaaaca tatgacgaag cagaagaatt attgaataag caattcgata
     6601 tgacattatt aaaaccaatg gcttaccaaa atacgtatgc cttggcagtc aagagatctt
     6661 ttgcgaacga acatcaactt aaaaagattt ccgatttggc agctatctct gatcagatca
     6721 aagcgggatt cacactggaa tttattgatc gttcagatgg atataaagga atccaagaag
     6781 catatggctt gaatttttca agtgtacaaa gcatggagcc aagtttgcgt taccaagcaa
     6841 tcaataatgg agatgtcaat attgtggatg cctattccac agatagccag ctcaaggagt
     6901 acgatctagt gactctagaa gatgatcgag ggttattccc agcttatcaa ggcgcaccgc
     6961 tgatgaaaaa agattttgct aaagaacatc ctgaaatcgt taaagcactg aataaattaa
     7021 gcggaaaaat aacagaggat caaatgattg atatgaatta ccaagtcaat gtcgaaaaga
     7081 aaaagccgac agaagtagca cgaatcttcc tgaaaaatga aggattagta aaggaggaag
     7141 atcagtgact aaattaatcg agttcaaaca tgtgcaaaaa aagtatgatg gaaagtatgt
     7201 catcgatgac ctaaatctgg caatcaaaaa aggagaaatc tttgttttgg ttggtccttc
     7261 cggtagtgga aaaacaacta ctttgaaaat gatcaatgga ttatctaaac catcagcagg
     7321 agacatctac ttcaaaggaa aatcgttaaa tgaatataat ctgcagaaaa tgcggtggaa
     7381 tatgggatat gtgctgcagc aaatcgcttt gttcccgacc atgacagtca agcaaaatat
     7441 tgaagtcatc cctgaaatgt taggttggga aaagcagaaa agagcagacc gtgtagatga
     7501 acttcttcaa aaagttggct tatctcctga tatttatcga gatcgtatgc ctagagagtt
     7561 gtctggagga gaacagcaac ggatcgggat catccgagct attgctgcaa gcccagatgt
     7621 gattttgatg gacgagccat ttagcgcatt agatccgatt tcaagaaact cattacaaga
     7681 attggttttg tccttacacg aagaattggg cacgaccatc gttttcgtta cgcacaatat
     7741 ggaagaagca atcaagctag gtgatcgtat tgcatttatg aaggatggag aaattatcca
     7801 atgtgacaca ccagagcagt tattgatgaa tcctaaaaac gactacgttc ggcatttctt
     7861 tgatgaaccg aaacagacga aagagtggcg agtagaagat ttagtcgtca atggatattt
     7921 cttaaatgaa ataccagaaa atgctcgtca acaagtttgc tttgatacgc ctatgaaaga
     7981 agtatattcg cttctttcgg tgtatccaag cattgtgata gtagaaaagc aaagaagtat
     8041 tgggtcgctg acaagtaaag aaatctttgc ttttttaagt cgagaggagg aaggaaatga
     8101 aaacatatga tgcaatcatc atcggttcag gtgtgagcgg cttaagtgct gcttatggat
     8161 tgaaagaagc aggtaaaaca gtactagtgg tcgaagaaga tctatggggc gggacttgtc
     8221 ctaaccgtgg atgtgatcct aaaaaagtct tgttaagtgc agtagaggca cgtaatcggg
     8281 taaaacaatt gagtggaaaa ggttttaacg agatcccaac tgccaactgg gaagaacttc
     8341 aaaaattcaa acgaacattt acggatccag tcccagaaag ccgaaaaaaa caattagcag
     8401 aagctgaaat cgatcatctt tcaggaaccg ctcgtttttt ggatgattca tctattgaag
     8461 tcaacgaaga agtcttccat gcggattact tagttttggc aaccgggcaa agacctacga
     8521 tcttaccagt ggagggaaag gaatacttga aaactagtgc agacttttta tcattgcctg
     8581 tactgcctaa agaaatcatt tttatcggtg gtggttatat tgcttttgag ctagctacga
     8641 ttgccaatgc tgcagggagc aaagtaacca ttgtccatca taatcaacgg ccattaaaag
     8701 aatttgaggc gtctttggta gaggaagccg tccatcaaat ggaagcatct ggcattcagt
     8761 ttgcttttgg tgttgaaacc caaaagatca tttcagaagg tactcgctat cgtttagtcg
     8821 gaaaagagac ggaattagta gcagatatga ttttttgtgc gactgggaga cagccgaata
     8881 cagaatcttt agcacttgag caagcgaata tcgtctttga taaacacgga attgcagtca
     8941 atgattatct gcaaacgagc aatccaaaaa tttttgcgtg tggagatatt gtttcaagga
     9001 aaaccccaaa attgacacca gtcgccactt ttgaaggaaa ttatgtagct aagcggataa
     9061 cggatgcaac aagcgaacct atcaaatatc cgattatacc tacgatcgtc tacgccagtc
     9121 ctaaacttgc tgaagtagga gttacaaaaa gtcacgcctc ttcgtcagat caagtggtag
     9181 agatggatct taccagttgg ttcacctatc atcgtgtcaa tgaaccagtt gctaaggctg
     9241 aattgacctt tgaccagcaa aattatttga ttggtgctgc agtgattagc gagcaagcag
     9301 acgaattgat cgatgatttg acactcgtca tcaatcaaaa attgactaaa aaagagttgg
     9361 atagctatat catggggtat ccgacattgg caagtgatct ctcctattta ttgaaataat
     9421 cagcatgata actaaagcgc ttgttgcttt ttccttcttg tgctacactt taaaaaatga
     9481 atacctgttt ctggggatgt cgtagacgac atgagagtaa gttgtgaact tagacccatt
     9541 gaacctgtca gttaatactg gtgaagggag aaacatagca gacgcttgaa gcgggttact
     9601 atgtcgtgcc catttcaagc gttttttgtt attcatatta gaagaaagga ggtaacaaaa
     9661 tgaagaaaaa tctttatcca gcagtcagta tggcattcat tttaattatt tggcagattg
     9721 ttgtcatagt ttggaaaata cctgccttta ttttacccgg acctgctgct gtattttctg
     9781 cattagcggc agatgcacaa gtattacttt cccattctgt cacgacatta aatgaagcag
     9841 tgcttggttt ggtaatagct agcgttcttt cttttattac tgccttggca atggattatt
     9901 ggaagttttt agaaatgtct ttttatccgt tactagtgat ttcacaaatg ttgccaatca
     9961 tggtgttagg gccattactt accctatggt ttggtttcgg tatgcttcca aaagtgatcc
    10021 ttgttgtctt gataagctat tttccgatcg tagtttcttt ttcggataca ttgaggaaag
    10081 tatcaaaaga gcaaatcatc tttttgaaaa cgatgggggc agatacaggg aagatttatc
    10141 gaatatataa aataccagcg ggtttatccg gcttcttttc cggtttaaaa gtggctgcta
    10201 cttattgtgt cagcggagca gtcgttggag aatggctaag cgctcaagca ggcttagggt
    10261 attacatgat acgcgtaaaa aacagctacc agatcgataa agtttttgct gcgatcattt
    10321 gtgtcattct attgagttta ttgctgaatg gcgcttgttc attattgaaa aaagggtatt
    10381 atcgtatgct ttatattttt taagagaagg ggatcaaatg aaaaaattat ggcttttatt
    10441 accgctactg ttgcttttat ccgcttgtgg cactgcaaaa gaaacaagta gcaaacaaga
    10501 aataaaggat ttgaaaaaag tgacacttgt tttggattat gtaccgaata ctaatcatac
    10561 tggcatctac ctagcaaaag aaaaaggcta ttataaagag gctggtttaa atgtccaaat
    10621 catcgaacca ggagataatt cgacaagtat tggtctagta ggtgcggaca aagcacaatt
    10681 tggtgtaagt tatcaagaag atgtcactta tgctcacgca gatggacaga atataccagt
    10741 caaagcaatt gctacagtga tcaaacataa tacttctgga ttcgccaccc tatcagacag
    10801 taacatccat tcaccaaaag attttgaagg aaaaacatat gcaggttggc aaagtccaag
    10861 tgaagaagct gtcttaaaag ctgtgatgga aaaagacggc ggggactttt caaaactgac
    10921 aatggtagga agcaatggag aagggccaga aagcttagga aaatcctcag atatccaatg
    10981 gtactttgaa ggttgggata tgatcaaagc aaaagaagcg ggtattgaac taaactatat
    11041 cccattgaaa gaattagatg aacggttaga ttactatact ccggtaatta ttacgaatga
    11101 tcaactgatc aaaagtgatc cagaattggt acagtctttt atggatgcca cgaaaaaagg
    11161 ctatcaggaa gctattaagg atccaaatga cagtgctaaa ctgttgcaaa aatatgcaaa
    11221 agaaaatgat cggacgtttt tagaagaatc acaagcattc ttgtcaaaaa attatacgga
    11281 tgatcctgag aattggggat tgatggaaga aaaagtttgg cgcaattata cagcttttat
    11341 gcaggaaaac ggattgatca agcaagatgt accttcgcaa gaactattca ctaatcagtt
    11401 tataaaatag gagtgataga tatgtcaatc caaatcagcc acttgaatgt agtcattgaa
    11461 aacctgaaaa tattagaaga catccatttc actatcccta aagatacctt tacatcgatt
    11521 gtcgcgccaa gcggagccgg taagtcaaca ttgctgaaaa ctttaacagg agtccagcct
    11581 atgacttctg gaacgatcaa agtagacgat caaaaagtaa ctggtctcca tacagcattt
    11641 agttatatgc cgcaagaaga tatgttactt ccttggctga gcgtctatca aaacgttacg
    11701 ttatatcaaa aaatcaatca tctaacaatc aatgaagaac aagtaaggga ttatctggat
    11761 atctttgggt taaacggcta tgaacatttt ttgccggaac aattatctgg cgggatgaag
    11821 cagcgaacgg ccttgttacg aacaatcatg aatccttctt cttacttgct tcttgatgaa
    11881 ccatttggag cgttagatgc tatgaccaga gggcagatgc aagactggat gttgaagctg
    11941 cctaaaaaag caaagcggac tactttactt gtgacgcatg atatcgaaga agcaatttat
    12001 ttatcagaca ggattcttgt gctttccgcc cgtccggcac atgtgattgc tgagattcaa
    12061 gtgcctgaaa aacgacggtc acgtgaatgg ctactgcagc aatcagagtt gaagcaaacg
    12121 atttatcagt tattggcagg tgaattagat gttaagtgat gctcattgtc atttagatgg
    12181 gagtcagagt ttagcactcc ttcagcagga acacacgatt ttgacgatca tcaattgtga
    12241 ttcgccagaa gaatggaagg aaaacagaca gcttgcagca agtaagactc aagccttaag
    12301 ttacggtatc catccttgga aagcagatag ctatacgttt gaacaagttg agcctttttt
    12361 gaaaaaagca aggatcatcg gtgaaatagg gttagataat atttggacga atgtaccgat
    12421 gactacacaa aaaaaagtat ttgagcgaca gcttgctttt gcagccataa atgagaaacc
    12481 tgttgtactg cacacaaaag gatgcgaaaa agagatctta aattatatcc aagcttaccc
    12541 taatcactat ttgatccatt ggtattcttc tttggactat caacaagact atattgattt
    12601 aggctgttat ttcagcattg gtcttgattt gaaaaagaat ccagcagtct ggcaactagc
    12661 taaagcagtc ccaatcgacc acttacttat tgaaacggat ggactacatg cagcagagtg
    12721 ggctttaggg gggatttttt cagaaaaaga ttatgtctca ctgcttgaag aacagttaaa
    12781 gacaatcgct gagatcaaag agctatcagt tgaagaagtt aaaaaaaata cttatcaaaa
    12841 cttggaacgt ttcattagat taggtaaata aaaaagagat gtgacacaac tagtgtcgca
    12901 cctctttttg gctttctttg tataaaagta aactgtttga ttaataaatg aatcttagta
    12961 ccaaccgttt gcttcccaga atgctttcgc cgcttcccaa gaaccataac gtgaagtaac
    13021 atattgttct gctactcttt cttggtttgc agcagagtag tcaccattta agtaagaaga
    13081 atctaattgg taacgtccga tgtaacgtcc gttcgttgct gtgtaagaac cgcttgattc
    13141 tttttgtgcg atccattctt ttgcagagct tgtgcttgct ggagtagcag cagtttctgt
    13201 tacagcagga gcaggttgtg tttcagcaac aggcgctgct tgtttttccg tttgaaccgt
    13261 ttcttgtaca acaggttgtt cagcttggac tggtgcttct tgaacggctg cttgaggttc
    13321 agcagctgct tgtgcagagc cttctgttgg gattgttaat tgttgtcctg agta
//
LOCUS       DAFCLG010000069        13146 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFCLG010000069 DAFCLG010000000
VERSION     DAFCLG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.069"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(77..149)
                     /locus_tag="LND56_001816"
     tRNA            complement(77..149)
                     /locus_tag="LND56_001816"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="LND56_001817"
     CDS             530..1099
                     /locus_tag="LND56_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBK6743735.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="LND56_001818"
     CDS             1086..1946
                     /locus_tag="LND56_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6743736.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="LND56_001819"
     CDS             2136..2840
                     /locus_tag="LND56_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743737.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="LND56_001820"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="LND56_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBK6743738.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="LND56_001821"
     CDS             4677..5993
                     /locus_tag="LND56_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743739.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="LND56_001822"
     CDS             5994..6863
                     /locus_tag="LND56_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBK6743740.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="LND56_001823"
     CDS             6918..7727
                     /locus_tag="LND56_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6743741.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="LND56_001824"
     CDS             complement(7830..8459)
                     /locus_tag="LND56_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK6743742.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="LND56_001825"
     CDS             8629..9921
                     /locus_tag="LND56_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6743743.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="LND56_001826"
     CDS             complement(9997..10659)
                     /locus_tag="LND56_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6743744.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="LND56_001827"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="LND56_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK6743745.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="LND56_001828"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="LND56_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK6743746.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DAFCLG010000070        13120 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFCLG010000070 DAFCLG010000000
VERSION     DAFCLG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.070"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(143..1729)
                     /locus_tag="LND56_001829"
     CDS             complement(143..1729)
                     /locus_tag="LND56_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK6743747.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            1980..2204
                     /locus_tag="LND56_001830"
     CDS             1980..2204
                     /locus_tag="LND56_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBK6743748.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2346..4097
                     /locus_tag="LND56_001831"
     CDS             2346..4097
                     /locus_tag="LND56_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6743749.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            4097..5881
                     /locus_tag="LND56_001832"
     CDS             4097..5881
                     /locus_tag="LND56_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743750.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6216..7067
                     /locus_tag="LND56_001833"
     CDS             6216..7067
                     /locus_tag="LND56_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6743751.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            7164..9050
                     /locus_tag="LND56_001834"
     CDS             7164..9050
                     /locus_tag="LND56_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6743752.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9091..9993)
                     /locus_tag="LND56_001835"
     CDS             complement(9091..9993)
                     /locus_tag="LND56_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6743753.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            10223..11626
                     /locus_tag="LND56_001836"
     CDS             10223..11626
                     /locus_tag="LND56_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6743754.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            11623..13059
                     /locus_tag="LND56_001837"
     CDS             11623..13059
                     /locus_tag="LND56_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6743755.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     ENEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
BASE COUNT         4184 a         2288 c         2855 g         3793 t
ORIGIN      
        1 atacacggtg ttccagaagt aacttcttcg gaaataagcc gaaatttcac aaaaatttga
       61 aaagcaattt tcttaaattc cttcttattt cttgaagctg cccacttctg tcacaacctc
      121 ctttgtgtaa aagaatcagt ttctattcga tttcttctat cgtttcttgc aaacgctgtt
      181 catatttttt gtttccttta tataaatcac caatcgtcca tgccaacagg agaaggacaa
      241 gaacaatgat tacataggcg atttgatcag ccaatgctag ttgagctgag gtggcttgct
      301 cgccgaagaa actttcgatt tgacctggta atcctcgtaa gttcaagtaa gtcagactaa
      361 tcacagagaa ccagccaaga agttttacaa caaatgaatt tttgaatcgg ctccccatct
      421 ccgcttggct attggtcatc atcagtaaag gaatcatcga gaatggcaat gcaaaagcca
      481 gaaaaacttg ggattcattc atcaagttat tcaaagcggt atgttcttcg atcgttcctt
      541 ttgtacttgt aaacaaaaca caaatcaata caggaatcac agaaagcaat cgtgtgacta
      601 gtcttcttag ccaaatcggg atacgcatat ggatgaatcc ttccatgatg acttgaccgg
      661 tcaacgtacc agtaatagta gagttctggc cagaagcaag aagtgctaca gcaaataaag
      721 tagacaagat accagatttt gctacagcaa tcaagatacc attgctcata gctgatggat
      781 cagataaagc ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg gttttgaata
      841 cagcgacacc catgatcaat agcagtgcat tgactaaaaa agccattgac aactggatat
      901 tagagtccca agtagaaaag cgtaccgctt gtgctacatc ttcttcgtct tggtgatcga
      961 tcttccgagt ttgagaaact gctgaatgaa gatataagtt atgcggcatg actgttgcac
     1021 caataatccc taacgctcca gtcagcggag tttcaccacc tatgacaggt gagtctgaga
     1081 atgtatgact gttagggata aagccagcaa ataaactgtg ccaatcaggg ttagacaagc
     1141 caacttgata gacgaaaacg aataaaatga cgaaaatcaa gcagacaacc aaagcttcga
     1201 ttttacgaaa cccgactttt gtcagcaata aaaggacgaa aacatctaac acagtgataa
     1261 atacggcaat taccaatgga atatcaaaaa gtaagtagag ggcgattgct gcaccaatga
     1321 cttctgcgat gtctgttgcc ataatggcta attcagtcaa gatccataaa atgacaccca
     1381 atgtcttact tgtccgtgct cggattgctt gggccaaatc catttgggtc acgattccta
     1441 attttgctgc catatactgt aaaagcatag caattagact agacatcaaa ataactgaca
     1501 tcaataaata ttggaaattt tgtccaccag tgatcgaagt ggaccagtta cctggatcca
     1561 tataaccaac tgctactaat gcacctggtc cagagtaagc aagaagtgtt ttaaaaaaac
     1621 tttttccctt tggtacatcc actgttccat ttacttcagc taaagataaa ccatttgtat
     1681 gttcaataag tcgatgctta tgaaattgtt tgttggattg attattcaag aaaaaaccac
     1741 ctttcctatt tatctacgat tacattctac acctcagaaa gaaaaaatta aagaaaaaga
     1801 ttcggtatgc ctaatttttt ttcgtggcgt gtcttgctgg ttaatgaaag aaattttgtt
     1861 cttctctaaa aaaaccacta ttttttgcat gaaactaaag aatacgcctt tcttttttca
     1921 ataaacttta gtaaaataaa tcattgagag caattcggaa atagaaagga gagatttata
     1981 tggtatcaac aggtattgtc gtacttattg ctattattgc tttgctaatc ggcgcagtag
     2041 gtggcttttt cctagcgcgt aaatatatga aagactattt agaaaaaaat cctcccgtta
     2101 atgaggacat gttgcgatca atgatgatgt caatggggca aaaaccatct gagaagaaaa
     2161 ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa ataggttctg acccaaagac
     2221 tttttgcggg ctgatttttt cagtccgttt tttatttcgt aatttgttag actattcaga
     2281 ctattcagca aaaaagacaa ggacagacaa tcctatgctc ttgataaaga caaaggagga
     2341 gtaaaatgtc gatttttaga aaattaggtt ggttttttcg tcaggaaaag aaacattatt
     2401 taattgggat tttctcccta ttcgcagtgg ccttggtaca gttagttcct ccaaaagtga
     2461 ttgggatcat cattgatgag atcgccgata agaatttatc aataaagatc atttcttggt
     2521 ggatcttact attgatcctt gcagctttct tacaatatct ttttcgttat atctggcgga
     2581 cgaatatttg gggaagtgca gctcggttgg agaaagaact tcggcagcag ctattcaaac
     2641 attttacaga aatggacaat ctcttttacc aaaaatatcg gactggagat ttgatggctc
     2701 atgcaacgaa tgatttgaat gcaatccaga atgtagcagg agctggaatc ttgacctttg
     2761 cagattcagt gattacgggc ggaacaacga tcattgcgat gattttgttt attgattggc
     2821 gcttaacact gattgcgctg attcctctgc ctttgctagc cgttacttca cgagtgctgg
     2881 ggtcaaaact ccacgatgct tttcgtgatt cgcaagaagc tttttctagt atcaatgata
     2941 agacacaaga aagtatcact gggatcaaag tcattaaaac atttggacaa gaaaaagaag
     3001 atctcgcgga ttttgaagaa aagattgaag atgcaattat taaaaacaaa cgagtcaatt
     3061 ttttagactc cttatttgat ccattcatta ccttgattat cggtctgtct tatgttgcaa
     3121 caattatttt aggtggaaag tatgtcttgg aaggcacgat ctcgattggg cagatggtct
     3181 catttattag ttatatcggt atgcttgttt ggccgatgtt tgcgattggc cgattgttca
     3241 acgtattgga aagaggaaat gcttcttatg atcgcgttaa tgaacttttg catgaaaaga
     3301 cgcatatcgt ggaacaactg ggtgcagtgc agaccccggc aaaaggcgag ctgaatgtag
     3361 ctatttcctc ttttaactat ccgaaagaag aatcaccagc attagaacaa atcaaatttg
     3421 cgatccaaga aggagaaaca ttaggtattg ttgggaaaac tggtgcggga aaaacgacaa
     3481 tcatcaaatt actgatgcgt gaatacgatg attatcaagg gaaaatccaa tttggcggac
     3541 gttcgatcaa agaatattca ttgaatgctt tattaggatc aatcggttat gtgcctcagg
     3601 atcacttcct gttttcgatg accattcgag ataatatccg tttttctaat ccaaaacttt
     3661 cagaagaaga agtagagcat gcggcttctt tagcatttat caacaatgaa atcaaggaat
     3721 tgccaaaagg ttatgatacc ttagttggag aacgtggagt ctctttatct ggcggacaga
     3781 aacaaaggat ttcgattgct cgagcattga tcaaaaatcc tgaattgctg atcttagatg
     3841 atgcactttc tgcagtagat gccaaaacag aagaagctat cttgcataat ttaaaagaaa
     3901 taagaaaaga gaaaacaacc atcattgcag ctcaccgttt aagcagcgtg atgcatgcaa
     3961 aagaaatcat tgttgtcgat caagggaaaa tcattgaacg aggaacccac cacgagctgc
     4021 ttgcattagg tggctggtat gaacgaatgt gggaaaaaca gcaattagaa gcgaagatag
     4081 aaggaggcga ggcctgatgg atgaaaatta tcaatcggaa tggacaaaat cgattccact
     4141 taaagaacag aagaagatca tcaaacgact gatgaagttc gccaaacctt ttagaagaac
     4201 gttcatcgtt gctatcttat ttgcgtttgc cctgtctgtg atcaatgtcc tattgccaag
     4261 gatcatccaa acttttatgg atgatcatct ggctaaacag tcagctacga cacaagttat
     4321 tttgtttttt gctggtgtgt acttgtttgg agtcatagtc aaaagtatca tttggttttt
     4381 ccaatggtat ttgtattcca tggcttcatt gaagacctat cagtatgttc gggtaagatt
     4441 atttgaaaag ctgcatacac taggtatgcg ttattttgac cagacaccgg caggatcgac
     4501 cgtttctcga gtgacgaatg acacagaaac gctatttgaa ttttggtatg tctttttgat
     4561 ggtattgact gggatctttg cagtcgtttc ttctttcgtt gccatgtttc aaatcaatgg
     4621 agaaattgcc ttgtggactt tggtgttctt gccgatttta ggaatcgtca tctggtatta
     4681 ccagaaattc agttctcgta tttaccgaaa tatgcgggaa aaattaagtc agctgaacac
     4741 gaaattgaat gaatcgattt ccggcatgcg tattatccag cagtttcgcc aagaagctcg
     4801 tttagctaaa gaatttgaac aagtgaacaa cgagtattta gacacgcgct atgcgatgat
     4861 caaaacgaat tcgattctgt taagtccgat catcaatttt ctctatgcat tggcgattgc
     4921 gttggcgttg actttgtttg ggatcgatgc gttgcattct cccgtagaag ttggactgat
     4981 ctatgcattt acgacctatg tccagggatt ttttaatcca atgtctcaaa tgatggactt
     5041 tttgagcacc tttacagatg ggatggttgc cggtagtcgt attttgaaaa tcatggatac
     5101 agaagaatat gcaccgcaac aaaatcctgg agcagatcaa gcgattactt caggtaagat
     5161 cgagtttcgc aatgtaagct tttcttatga tggagagcac aatgtattga acaatatctc
     5221 ttttgtcgct aatccgggtg agaccgttgc attagtgggg cacaccggaa gcggaaaaag
     5281 ctcgatcatc aacgtattga tgcggtttta cgaattttat gaaggccaaa tcttgattga
     5341 tgatcatgac attcgagaat atccaatgga agagttacgt aagaaaatgg gattggtctt
     5401 gcaagatgcc ttcttgtttt atggagatat tgcaggaaat atccggatga tgaatccagc
     5461 tatcacagac gaacagattc gccatgcagc tgagtttgtt caagcaaaca agttcatcga
     5521 tgaattacca aaaggatatc actcaaaagt gatcgaacac ggtgccagct attctagtgg
     5581 acaacgacag ctgatcactt ttgcgcgaac aatcgtgaca gatcctaaaa tactagtatt
     5641 ggatgaagca acagcaaata tcgatactga aaccgaagga ttgatccaag aaggattggc
     5701 aaaaatgcga caaggacgga caacgattgc gattgcccat cggctgtcca cgattcggga
     5761 tgcagaattg attttagttt tagacaaagg tcatatagta gagcgtggga atcatgaaac
     5821 acttctagcg caaaatggtt tgtatgcaga tatgtacaaa ctgcaaagca gtggtgtgta
     5881 aaaagaggtt gtgacagtag catagtagca ttttgtccta agcgctagag tgaaaataaa
     5941 acagagcttc ccagattctt catctgtgag cgttagtggc gtagtcaccc gatttaaaaa
     6001 agacgacgac agctagtcaa aagtgcctaa tagccactaa gaaagcgttg acaaaagcgt
     6061 ttgctttcga taatataaag aaaacgatac aacttggata gtgattgcct gatgcaagct
     6121 aaacctgatg agtctcccat ggttccatgg acggcttttt gggtttttct ttttgttttg
     6181 aatacagccc tatatatcat ggaaaggagc aaaacatgct gattgagaaa gtactgaata
     6241 acaacgtcgt aataaccagg gatgaaagaa atcaggaaat gatcgtgatg ggaaaaggtt
     6301 tggcattcaa aaagaaagtg ggtgacccga tttttgactc atgcatcgac aaaaaatttc
     6361 agttgacaaa tgacgggctt tcacaacagt tccaagaatt ggcacaagcg attcctcttg
     6421 agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctcta caagtccagt
     6481 tgaatgattc tgtgtatatt gcattgacgg atcatttgta tacggctatc gaacgtcaca
     6541 aaatgggaat atttgtacca aatgtattgc tgtatgatat caaacaacta tttcctaaag
     6601 agtatgccat cgggaaaaaa acagtgaaga aaatctcgga taaataccac atcaagttat
     6661 cagaagatga agccggattt atcgcattgc atttagtcaa tgcacaaggc attcaaagcc
     6721 aacaaaacaa tacctacctg tttactataa caatacagga tattctaaat atcgtccgct
     6781 attactataa gattacattt gatgaagatt cagtccattt ttttcgattc gtcacacact
     6841 tacgtttttt tctgacacgc gtcctagatg gagaaaaatc agaagtggga gaagtttctg
     6901 aggaattgtt tcaggttatt caaaaaaaat ataaggatac gtttggttgc gttgagaaaa
     6961 tagcagactt tttagccgag acgttcaact accagatgtc cacggacgaa aaaatgtatt
     7021 taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaata aaaaaaggag
     7081 gataaattaa caagaacgta tgaaataagg gtagtgacat tcagttggct aaaccttcca
     7141 ctaatatatt ggagggaata gaaatgggaa aataccaaga attagcgaga aaaatcgtag
     7201 aaaatgtcgg tgggaaggaa aatatcaatg gtttgacaca ttgtatcaca aggcttcgtt
     7261 tcaaattaaa aaacgaagaa aaagcaaatg atgagatctt gaaaaatatg gacggtattg
     7321 taacggtaat gcgagcggga gggcagtatc aagttgtcat cgggaatcat gtgccagtcg
     7381 tgtttgaaga ggttataaaa gcaggaaacc ttacttttga tgaggctgtt tcaacagaaa
     7441 aaatgagacc gtttgatatg cttattgatg gaatatcggg ctgtttccaa ccattccttg
     7501 ctatcttagc tgctggcggg atgattcgtg gactgacagc attccttgtc ttcttaggcg
     7561 cgtttgaccg cggttctggc acatttgtta tgtttgataa tatcggtgat tctgtattcc
     7621 aatttatgcc tgttattatc ggtttaacag ctgcccgtaa atttaaagta aatgaattcg
     7681 tagggatgtt gcttggcgca gcattaatga atcctagctt gtcgctggaa gctttgagtg
     7741 gtgcagcaga agcaccacta acgaccatat tttctggcac tatttttgaa gcacctattt
     7801 atcaaacagt attcggcatt ccctggattg cgcgtaacta cgcttcttct gtcattccca
     7861 ttatctttat tgtgttgtta gctagccaaa ttcagaaacc aattaaaaag ttagtacctg
     7921 agatgatcgc gaatttcttc gttccattct tcaccgttct tattacaatg cctttaggtt
     7981 ttttactcgt tggaccagta ttcacattcg caactgatat tttaatggct ggatttgaaa
     8041 cacttttagc actttcgcca gttatttatg gagcaatcgt aggcttcttc tggcaaattc
     8101 tggtgatgtt tggtctccat tgggcaatcg taccaatggg attgatgcaa ttttctgtga
     8161 atggttggca aaatattatg acacctgttg cagtagtgtc ttttggacaa actgcagcac
     8221 ttaccgcact ctactttaag cttagaaatc caaaagacaa agcaattgca ataccagcca
     8281 ttgtttctgg tattgtgggg attacagagc cagctattta tgggtttaca ttaccacgta
     8341 aaaaaatatt tatttttact tgtgtcggtg gtgcgttagg gggcgcgtat agtgggttaa
     8401 tgaatttaac ttcttggaat caaggtggac taggcatctt tacaattccc aactatatta
     8461 gaccagatgg tgatttaaca gatgtgatta atgtattgat tgggatcgcc attgcgatgg
     8521 tggtatcctt tgcgctgact tttttctttt ggaaggagga agcaggtgaa acagatgaca
     8581 gacagaaaag atcaggtaaa gaaattgtaa aaacaccgat acaaggacaa atcgctccac
     8641 tgaaagcagc aaaggatgca gcttttgcac aaggaacact aggtcgagga atcttgatct
     8701 accctgaaaa aggagaagta cgtgcccctt ttgacggaac agtcatgacc ctgttcccca
     8761 cgaaacatgc aatcggaatg gtttctgaaa caggtctgga attgctgatt catgtcggat
     8821 tggatactgt tcagttggaa gggaaatatt ttgaaagctt ggtgcagcaa ggagaaagag
     8881 tgaaacaagg cgatctgttg ctccgcttcg acaaaaagaa aatcgaaaag gaaggataca
     8941 gtttagaaac acctgtaatc gtcaccaatt acatggatta tttggatatt ttggaaaatc
     9001 aaggggagac ggtcgggcca gacgatgcac ttatcaccgc actgacctga acggttaggt
     9061 gaaagaatgg aatagactta actattcttt ttagtccact tctttatttt caaaattgaa
     9121 aggctctttc atgatggctg agctgatagg gcgtttatca aatttttgtc ctacagaaat
     9181 tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag
     9241 aagataacaa atcgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgttcgcg
     9301 tgtcgtaagt cgcaaagcgg ctgtactcat tgaggatagc gttgaatcac cgatactggt
     9361 caacgctaaa attggaacat gattttgttt caatatttct gcggtacgaa ggatcacatt
     9421 gttttcaccc gtataagaaa tcagaatccc gcacatatca ctcgtgcaat tgtacgcttc
     9481 atagaacgat tcaccaaaag tggtgacaat cgtgacatcc attttgatgc gcttcatttt
     9541 cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaaaatca cgattttttt
     9601 tgcttgaagc attagttgtt tggctttttg cagttggtca tgatgaatga gagaaagtgt
     9661 atcttgaatc gtcatctgtt ctaaagaagc cattttattt gcgatcgtca taataccgtc
     9721 tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt
     9781 ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac
     9841 acggatgagt gtggacgggt ggacataaca tcgctcagca atctcttgga tggtcatttc
     9901 gccaagtgct ggagaatcgc ttaagagaaa ctgaatcact tcattttctg aaggagaaaa
     9961 ggagatttct ttgatttttt cacgtaataa catgtagata ccttcttcta gtttttttac
    10021 gtaaattata acttttttga aaatgatttg taaacggatt tatgttatat agcttaataa
    10081 agtcattttt tgacaagata tttacaaaaa tccagtagaa aacactaact tttgtaaatg
    10141 ttactggaaa gaaacgaatt tctattgttt gttttgttta caaaaaagat aataaaagtg
    10201 taaaaagcaa aggagtgaga acatggcaaa aggaatcaaa atcgctacga ttggtggagg
    10261 cagcagttac acccccgagt tgatggaagg atttatcaaa agatatgagg aattaccgat
    10321 acgagaaatc tggctcgtag atgtagaaga aggaaaagaa aagcttgaga tcgtcagtca
    10381 gatgtctcag cgtatgtggg atgcttctcc gtatgaagtc aagatctatg caactttgga
    10441 tcgggaagaa gcattgaaag atgcggattt tgttacgact cagtttcgtg tcggtcaatt
    10501 agatgcacga atcaaagacg agcggatccc tagttattat gggatgattg ggcaagaaac
    10561 aaatggtgct ggcgggatat tcaaagcttt tcgtaccgtt ccagtggttt tggcaatcgt
    10621 tgaagacatg aagcgtttat gtccaaatgc ttggctgatc aatttcgcga acccaagcgg
    10681 gatggtgaca gaagcagtta tcagatatgg caaatgggat aaagtgatcg gattatgcaa
    10741 tgtgccagta atggcgacga tgacagaacc agcaatgctt ggcaagaaaa aagaagagct
    10801 gatctacaag tttgccggtc taaatcactt ccattggcac aaagtagcag ataataaagg
    10861 aaatgaccgc acagcagatt tgattgatcg tttatacgat gaagacaatg gattgccgaa
    10921 aaatatcttt gacatcccgt ttttcaaaga acaattaaaa caaatgaaca tgatcccttg
    10981 tggctaccac cgctattact accgtgaaga agaaatgttg gaacatgctt tagaagaata
    11041 tcaaacgatt ggtacaagag cgcaacaagt gaaagaaaca gaagcagagc tgtttgaatt
    11101 atacaaagat ccagaattgg atcataaacc tgaacaattg caaaaacgag gtggtgctta
    11161 ttattcagat gctgcctgtg aaacgattgc ttcgatctat ggaaataaag aaacacagat
    11221 cgtggtgtct acaaagaatg atggagctgt tcctgatctg ccggcagatt gtgtagtaga
    11281 agtcactgcc tatgttggcg cacaaggcgc acgaaatgta gcgtttggtt cccttcctgc
    11341 tgctgaaaga ggctggttgc aagtaatgaa gaatatggaa ttgctgacga tcgaagcagc
    11401 cgtgactggt gattatggaa cagctttaca agcgtttacg atcaatccgc ttatacgatc
    11461 aggaaagaca gcaaaacgga tcatggatga gttgtttatt gcacatcagc agtatttacc
    11521 gcaattcaag gaaacaatca gccggttaaa agcagaagga atcgaagtcc aagatgaact
    11581 ggcaagagaa ttgtcaagca atacacatgt aatggaggaa gtatgatggg attcagaaaa
    11641 gattttttat ggggcggtgc tacagcagcc aatcaatgcg agggtggtta tgatgaaggc
    11701 ggacgtgggt tagcgaacgt agatgttgtg ccaatcggag aagaccgttt tcctgtcatt
    11761 actggaaaaa agaaaatgtt tgaatttgat aaagatcatt tttatccagc aaaaattgcg
    11821 atcgatatgt atcatcatta taaggaagat attgctttgt ttgcagaaat ggggttcaag
    11881 acttatcgga tgtccattgc ttggagtcgt atttttccaa tgggcgatga aaatgagcca
    11941 aatgaatctg gtttgaaatt ttatgaggat gtctttaaag aatgtcaaaa gtacgggatc
    12001 gagccgcttg tgacgattac acattttgat tgtccgatgc atttgatcaa agaatatggt
    12061 gcgtggcgtt ctagagaact cgttggtttt tatgaaaatc tgtgccgagt catcttcaat
    12121 cgctataaag gacttgtcaa atattggttg acattcaatg agatcaatat gatccttcat
    12181 gcaccattca tgggcgcagg gctgtatttt gaagaaggag aaaatgaaga acaaatcaaa
    12241 tatcaagcgg cacatcatga gttggttgca agtgccatcg caacgaagat tgctcatgaa
    12301 gtagatccgg agaacaaagt aggctgtatg ctggcagcag gagcatatta tccatacact
    12361 tgcaatcctg aagatgtttg ggcttctaga aaatcagatc gagaaaatta tttcttcatt
    12421 gatgtccagt cacgaggaga atatcccgct tatgcattga aagaaatgga acgaaatggc
    12481 attcagatca aaatggaacc tggggatgcc gcattgctga aagaacatac tgttgatttt
    12541 atctcctttt cttattattc ttctcgagtt tcgactactg acaacgaact cttggaacag
    12601 actgcaggaa atatctttgc ttctgtcaaa aatccatatc tggaagcaag tgaatggggt
    12661 tggcaaattg atccattagg cttgagaata acgatgaatg atatttatga tcgttatcaa
    12721 aaaccactat ttattgtaga aaacggctta ggtgcagtcg atacgcccga tgagaatggt
    12781 tatgtggaag atgactatcg aatcgactat ctggcagcac atatccaggc aatgaaagat
    12841 acagtagaat tagatggagt cgatctatta ggctatacga cttggggatg tattgattta
    12901 gtatcagctg gtacaggtga gatgaagaaa aggtacggat tcatttatgt cgatcgtgac
    12961 aacgaaggaa atggcacatt agcccgttcg aagaagaaat cattcaactg gtataaaaaa
    13021 gtaattgatt caaatggaga aaatctttcg aatgattgat caatgatttg taaaagtcta
    13081 ttgtttttca gcggattttt cgagtataat aaagctattc
//
LOCUS       DAFCLG010000071        12485 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFCLG010000071 DAFCLG010000000
VERSION     DAFCLG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.071"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(476..1063)
                     /locus_tag="LND56_001838"
     CDS             complement(476..1063)
                     /locus_tag="LND56_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743756.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1238..2560
                     /locus_tag="LND56_001839"
     CDS             1238..2560
                     /locus_tag="LND56_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6743757.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2715..3491
                     /locus_tag="LND56_001840"
     CDS             2715..3491
                     /locus_tag="LND56_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6743758.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3484..4053
                     /gene="rnmV"
                     /locus_tag="LND56_001841"
     CDS             3484..4053
                     /gene="rnmV"
                     /locus_tag="LND56_001841"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBK6743759.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4046..4930
                     /gene="rsmA"
                     /locus_tag="LND56_001842"
     CDS             4046..4930
                     /gene="rsmA"
                     /locus_tag="LND56_001842"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK6743760.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5381..6010
                     /locus_tag="LND56_001843"
     CDS             5381..6010
                     /locus_tag="LND56_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743761.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6134..7243)
                     /gene="ugpC"
                     /locus_tag="LND56_001844"
     CDS             complement(6134..7243)
                     /gene="ugpC"
                     /locus_tag="LND56_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBK6743762.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7452..7874
                     /gene="mgsA"
                     /locus_tag="LND56_001845"
     CDS             7452..7874
                     /gene="mgsA"
                     /locus_tag="LND56_001845"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK6743763.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8197..8733
                     /locus_tag="LND56_001846"
     CDS             8197..8733
                     /locus_tag="LND56_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6743764.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8773..10536)
                     /locus_tag="LND56_001847"
     CDS             complement(8773..10536)
                     /locus_tag="LND56_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743765.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10539..12254)
                     /locus_tag="LND56_001848"
     CDS             complement(10539..12254)
                     /locus_tag="LND56_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743766.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3914 a         2287 c         2522 g         3762 t
ORIGIN      
        1 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
       61 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
      121 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
      181 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
      241 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
      301 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
      361 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
      421 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
      481 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      541 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      601 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      661 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      721 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      781 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      841 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      901 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      961 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
     1021 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
     1081 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
     1141 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
     1201 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
     1261 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
     1321 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
     1381 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1441 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1501 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1561 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1621 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1681 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1741 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1801 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1861 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1921 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1981 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     2041 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     2101 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     2161 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     2221 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     2281 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     2341 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     2401 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2461 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2521 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2581 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2641 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2701 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2761 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2821 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2881 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2941 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     3001 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     3061 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     3121 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     3181 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     3241 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     3301 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     3361 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3421 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3481 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3541 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3601 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3661 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3721 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3781 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3841 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3901 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3961 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     4021 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     4081 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     4141 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     4201 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     4261 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     4321 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     4381 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4441 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4501 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4561 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4621 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4681 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4741 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4801 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4861 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4921 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4981 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     5041 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     5101 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     5161 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     5221 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     5281 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     5341 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     5401 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5461 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5521 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5581 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5641 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5701 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5761 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5821 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5881 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5941 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     6001 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     6061 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     6121 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     6181 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     6241 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     6301 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     6361 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6421 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6481 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6541 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6601 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6661 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6721 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6781 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6841 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6901 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6961 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     7021 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     7081 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     7141 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     7201 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     7261 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     7321 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     7381 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7441 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7501 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7561 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7621 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7681 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7741 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7801 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7861 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7921 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7981 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     8041 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     8101 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     8161 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     8221 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     8281 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     8341 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     8401 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8461 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8521 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8581 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8641 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8701 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8761 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8821 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8881 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8941 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     9001 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     9061 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     9121 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     9181 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     9241 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     9301 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     9361 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9421 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9481 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9541 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9601 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9661 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9721 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9781 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9841 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9901 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9961 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
    10021 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
    10081 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
    10141 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
    10201 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
    10261 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
    10321 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
    10381 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10441 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10501 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10561 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10621 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10681 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10741 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10801 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10861 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10921 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10981 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    11041 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    11101 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    11161 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    11221 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    11281 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    11341 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    11401 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11461 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11521 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11581 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11641 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11701 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11761 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11821 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11881 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11941 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    12001 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    12061 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    12121 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    12181 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    12241 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    12301 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    12361 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    12421 atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta atttttctat
    12481 aaatg
//
LOCUS       DAFCLG010000072        12281 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFCLG010000072 DAFCLG010000000
VERSION     DAFCLG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.072"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="LND56_001849"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="LND56_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="LND56_001850"
     CDS             complement(1437..2279)
                     /locus_tag="LND56_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6743767.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="LND56_001851"
     CDS             2604..3020
                     /locus_tag="LND56_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6743768.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="LND56_001852"
     CDS             3017..3487
                     /locus_tag="LND56_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6743769.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="LND56_001853"
     CDS             3501..4274
                     /locus_tag="LND56_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6743770.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="LND56_001854"
     CDS             4288..5100
                     /locus_tag="LND56_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6743771.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="LND56_001855"
     CDS             5154..6119
                     /locus_tag="LND56_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6743772.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="LND56_001856"
     CDS             6256..8979
                     /locus_tag="LND56_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6743773.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="LND56_001857"
     CDS             8992..9624
                     /locus_tag="LND56_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6743774.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="LND56_001858"
     CDS             9829..10056
                     /locus_tag="LND56_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBK6743775.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="LND56_001859"
     CDS             10125..10391
                     /locus_tag="LND56_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6743776.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="LND56_001860"
     CDS             10381..10650
                     /locus_tag="LND56_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6743777.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10829..12130)
                     /locus_tag="LND56_001861"
     CDS             complement(10829..12130)
                     /locus_tag="LND56_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6743778.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4006 a         1989 c         2409 g         3877 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga
    10801 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
    10861 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
    10921 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
    10981 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
    11041 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
    11101 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
    11161 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
    11221 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
    11281 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
    11341 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
    11401 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
    11461 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
    11521 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
    11581 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
    11641 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
    11701 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
    11761 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
    11821 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
    11881 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
    11941 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
    12001 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
    12061 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
    12121 gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt
    12181 ctgtatgttt tgttttttca tgctcaaaag ggaccgatag aatgattttc tatcagtcct
    12241 agaaattata gagccttttt tatttggtct tgaagaagaa a
//
LOCUS       DAFCLG010000073        12192 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFCLG010000073 DAFCLG010000000
VERSION     DAFCLG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.073"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(154..318)
                     /locus_tag="LND56_001862"
     CDS             complement(154..318)
                     /locus_tag="LND56_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743779.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            613..1941
                     /locus_tag="LND56_001863"
     CDS             613..1941
                     /locus_tag="LND56_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBK6743780.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2143..2976
                     /locus_tag="LND56_001864"
     CDS             2143..2976
                     /locus_tag="LND56_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK6743781.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2992..3729
                     /locus_tag="LND56_001865"
     CDS             2992..3729
                     /locus_tag="LND56_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBK6743782.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3884..4852)
                     /locus_tag="LND56_001866"
     CDS             complement(3884..4852)
                     /locus_tag="LND56_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK6743783.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5084..5917
                     /locus_tag="LND56_001867"
     CDS             5084..5917
                     /locus_tag="LND56_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK6743784.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5951..6790
                     /locus_tag="LND56_001868"
     CDS             5951..6790
                     /locus_tag="LND56_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6743785.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6816..7376
                     /locus_tag="LND56_001869"
     CDS             6816..7376
                     /locus_tag="LND56_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6743786.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7575..9296
                     /locus_tag="LND56_001870"
     CDS             7575..9296
                     /locus_tag="LND56_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBK6743787.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9461..10603
                     /locus_tag="LND56_001871"
     CDS             9461..10603
                     /locus_tag="LND56_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6743788.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10619..11830
                     /gene="thiI"
                     /locus_tag="LND56_001872"
     CDS             10619..11830
                     /gene="thiI"
                     /locus_tag="LND56_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK6743789.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2047 c         2532 g         3459 t
ORIGIN      
        1 agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt
       61 ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa
      121 tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa
      181 tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat
      241 gacattccca aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc
      301 agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc
      361 aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat
      421 acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc
      481 gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca
      541 tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg
      601 aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt
      661 caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct
      721 tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct
      781 acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac
      841 acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat
      901 tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca
      961 acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa
     1021 gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa
     1081 tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac
     1141 aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta
     1201 gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc
     1261 tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat
     1321 catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta
     1381 gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat
     1441 acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat
     1501 atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt
     1561 ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat
     1621 tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt
     1681 ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa
     1741 tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt
     1801 gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta
     1861 ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat
     1921 ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa
     1981 gagctggctt aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc
     2041 tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata
     2101 tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt
     2161 ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag
     2221 acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa
     2281 aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta
     2341 ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc
     2401 gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa
     2461 acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat
     2521 tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct
     2581 ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa
     2641 gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac
     2701 ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct
     2761 gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga
     2821 ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga
     2881 ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac
     2941 ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa
     3001 aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca
     3061 tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga
     3121 agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg
     3181 gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag
     3241 tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta
     3301 gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt
     3361 aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa
     3421 aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc
     3481 gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga
     3541 tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta
     3601 gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa
     3661 acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa
     3721 aataaataaa aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta
     3781 cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt
     3841 gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg
     3901 aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt
     3961 tttgactatt aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc
     4021 cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag
     4081 ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg
     4141 cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt
     4201 ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca gcggaaatgt
     4261 ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc
     4321 cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca
     4381 cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt
     4441 cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt
     4501 cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat
     4561 caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata
     4621 tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga
     4681 tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg
     4741 tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt
     4801 tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc attttacaga
     4861 cttccttacg aataattttg catagccgtt aaatcttatt gtatactata taaaagagtt
     4921 ttgctatcag tgaagaataa cgaatagcct aacttatcat aaaaaagctg accttattaa
     4981 aatggaaaag actcaacctt gacctcctaa tacaatggag tatctggttg agtttctttt
     5041 acaagaaagc agccaaggaa atcaatcacg aaggtggccg gaaatgatca gaatcggatt
     5101 aacatcgttt aaagaacatg acaaattaac tggtaaaaag aataacactc tttatgaata
     5161 tgcagggttt ctacccttag tggaattaga tactgcctat tatggaattc cccgaaagtc
     5221 ctcagttgag aattggataa gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta
     5281 tcaaggaatc agcggtcaag gagactggaa gccttattat cagaccgaag aagagatgat
     5341 tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt
     5401 agtccaattc ccctcatcat tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact
     5461 tagaaagtgg tttgagggtt atcctgtttc tatagaattg cgggattatt cttggtacag
     5521 cgatgaattt cttgaacgta caagaaactt tatgtctaga gccgcttttt ctttagccat
     5581 catcgatgag ccacagttgc tgaatacaac gattcctttt gatccatttg tcaccaatcc
     5641 gaactttgct ctttttcgat tccatggtag gaatgcgcaa ggatggcaag caaatgataa
     5701 agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc aagaactagc
     5761 acatgaagtc aaagtagttg attctaagac aaaagaggta ggtgtgattt ttaataataa
     5821 ttctggtggt gatgcggctg aaaatgcatt agcactgcaa aaaatattaa atatcaagcc
     5881 agatggactc aatccaaccc aaatggatct gttttaaggg cttattttat tcaagaaagg
     5941 ggcgaagtct atgatcaaag ctattttttt tgatattgac ggaacattgg tcaccaatcg
     6001 ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat gctcggaaaa acggcatttt
     6061 atgcggtgta gcaacggggc gcagcccagt aaagatcaaa gaaatcattg atgaattgga
     6121 attagatatg tatgtcgtgt ataatggtca actggttttt actgcagatc gcacgattat
     6181 cgaccaccct tttgaacaaa aagtattgga acatattgtt gaatttgcag atgagaacca
     6241 tcgacaaatc gtttttggtg cgagaaatcg gttagatgga tcaacgacga tgctattagg
     6301 acaatcgatt tttatcaagc gactagtcag ctttttaccg cgtaaattcc cggtacgtct
     6361 gatgaaaaaa atcctccagg tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt
     6421 agagatatta aaagaaccaa tttatcaatg tatattgctc agtccggagt cagaacagaa
     6481 aaagttagaa catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga
     6541 tatcatccca aaaggtggtt caaaattact aggcatccaa gctttcgcaa atgctgtcga
     6601 aattgaaatg gaagaaatta tggcattcgg cgatcattac aatgatatcg agatgctgaa
     6661 aggtgtagga attggagtgg ccatggggaa tgctcagatc gaagtaaaac atgctgcaga
     6721 ctttgtgact caatcaaatg aagaagatgg gatatacgaa ggactgaaac acttcggtct
     6781 gatttattag tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa
     6841 aagtagaaac attccacaaa acttttgatc caagaagacc agagataccc acggctttta
     6901 cagaaaatga agctcataca cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg
     6961 cagctacttc acctaataaa gaacaactgc atgaagctgt tatgctgctg cacgaagcat
     7021 tagataaagc agaagaaaaa atacttggta aaacacagtg gggaagtgat ccgctggttg
     7081 aacaagtaga tgcactgaca gatatccttt actttactta tggttctttt tcattactag
     7141 gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg aaattatttc
     7201 cagatggaaa acctcattat gaccctgtaa caaataaagt gttgaaacct gaaaactggc
     7261 agatggaata tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag attcaagaag
     7321 cgattgctaa gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag
     7381 agtttcacta tttttgttat ctttatacga tggaaaaaca aaagattttc gtttaagtta
     7441 gtgaatgcat gaaaatagta tgctataatc tagaatagat gtcttttgca cgagagacgt
     7501 attatttgga tttatgccaa gcagaacgcc atctgttgca taaaagatgc atactgaaaa
     7561 cggaggaacc ataaatggga aataatgtta tcatcggaat cgtcattgca atcattatag
     7621 tcgccattgt cgtctttttg gttagttact tgatgaagaa aaaaaaccaa gatcgattgg
     7681 atgccttaga aaaacgcaag gaagaattat ttgacttgcc agtgattgaa gaagtcgatg
     7741 atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa
     7801 aatggaatga catatctact tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg
     7861 aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag
     7921 ctacaatgga ccaaatggag aaacaagtga cggaaatccg tgaaggtcta aaggaattgc
     7981 gggaaacgga agaacgcaac tcactagctg ttcagcaggc acttgacgtc tatgaagaac
     8041 tgaaagaaaa agtcagagaa gacgagcatg aatacggacc agctcttttt gaattgcaaa
     8101 aacagatcaa agtgatcgaa aaagaattta cgcagtttgt gactttaaac acttctggag
     8161 accctgttga agcccgcgag gttttggata aagcagaaaa acatacgtat gaagtggaag
     8221 ctctgatgaa aaaaattcca ccgctgtatg aggagctcca taccactttc cctgaacaac
     8281 ttgaagaaat cagtgatact tacgagaaat tgatggaaga acaatatgta ttcccagaag
     8341 aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc
     8401 ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct gatttgattg
     8461 attcgttata tgatatctta gaacgggaaa tggaagctca gagatatgta aaaactaacc
     8521 aatcaacgat tgcagagtat atcaaacata caacaaagaa taaccgtcaa ttattgattg
     8581 agctggatca cacatctcaa agctatacat tgaaccacaa tgaaattggc cgtgtacgag
     8641 gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga acagatgatt ccacagatcc
     8701 gtcaacacga aattccttat tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct
     8761 tagaagatat cgaaaagcaa caagtagaga tcgacgactc attacatgaa ttgagaaaag
     8821 gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt caaattgcgc agtttgaaac
     8881 gttttgtaga aaaacaacgt ttgcctggct tgccgaatga ttacctagaa ttcttttttg
     8941 ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg
     9001 aagaggtcaa tcgtttagtt gctctatgtg aagaagatct agaattatta gataagaaga
     9061 cacatgattt agtagatgca gcagcattaa cggagcaaat gctccaatat gcaaatcgtt
     9121 atcgtcatac acacgaagaa gtgagagaag caattgataa aagctactat ttattcaata
     9181 aagaatatca ctatcaagaa gcattagacg aaatcggtac agcattagag cgagtagaac
     9241 ctggtgcttt caaacgcatc gaagattttt attttaatca tccagactta gtataaagca
     9301 aaaaagttag taaaccagca agagcaagac ggattattgt cttgttcttg tctttttttg
     9361 tatatttgaa aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac cttggacaac
     9421 cgttataata gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt ttgataacag
     9481 tgcaacaacc ccaatctttc cgcaaagctt agatgcatat gtaaaaacca gtcagcggat
     9541 tattgggaat ccatcaagct tgcatgattt aggcaatcaa gctaaccggt tgttgcagca
     9601 agcaagaaag cagatcgctg atttgatcca tgtagaacca gaagaaatct attttacttc
     9661 aggcggaaca gaaggagata actgggtatt aaaaggaacg atgatcgaaa aaagagaata
     9721 tgggaatcat atgatcattt caggagtcga acatccggcc gtcagcgaga cagcagaaca
     9781 attaaaagaa ttaggaatcg aagtaagtat cgccccagtg gataaacgcg gatttgtacg
     9841 tgtcgacgaa ttgaaagaat taataagaaa agagacagta ttagtctctg ttatggctgt
     9901 gaataatgaa gtaggatcga ttcagcccat ccaggaaatc agtgatcttt tggatgattt
     9961 tcctaaaatt catttccatg tagatgctgt tcaggcgatt ggaaaagtac cattctcgca
    10021 atggttgaca gaaagagttg attttgcaac attttcagct cataaattcc atggcccacg
    10081 gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg
    10141 agggcaagaa agaaatcagc gaagtgggac agaaaatgtt ccaggtattg tggctatggt
    10201 taaagcttta cgtttatcat tagagataaa ggaacaaaaa cctgagcaga cacaacgctt
    10261 aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt tttcagaagg
    10321 aactgactat gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct
    10381 cgtccatgcc ttagaagaaa aacaaatatt tgtttctact actagtgcct gctcctcacg
    10441 taaacatatg gcaagcagta cattgcacgc aatgcatgta ccaaatgatc tggctacatc
    10501 tgctgttcga atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat
    10561 tattttcaag cagctttata agaaattttc tgcaatcaat taaaagagga gtgttcttgt
    10621 gaaatacagt gaaattatgg tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa
    10681 aagctttatc atgcaactag cacaaaatgt cagacaagct ttgaaagact ttccagaaat
    10741 caaaatccat gctgatcggg accggatgca tcttttattg aatggcgcag acgggcagct
    10801 agttattcca aaactagcaa aaatatttgg gatccaaaat ttctctccaa gtattcgtgt
    10861 agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag aaatttatac
    10921 tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat caccaatttg aattgacaag
    10981 taatgaattg aatcaaacat taggtaatgc tgtttttgat attttcccgc atatccaagt
    11041 tcaaatgcgt caaccagata ttccattacg tgtagagatt cggcgtgatg gcgcttatct
    11101 atcgtatgaa acgatcaagg gagctggagg attgcccgta ggaacaagcg gaaaaggaat
    11161 gcttatgctt tctgggggga ttgattctcc tgtagcaggg tactttgcca tgaagcgggg
    11221 agtggaaatt gaagctgttc attttgctag tccgccgtat acaagtgaac aagcactgca
    11281 aaaagcaaaa gatctaacag ccaaactagc accttatgtg ggaacgatcc attttatcga
    11341 agtgccattt accgagattc aagaagaaat caaaaagtca gtgccacaag gatattggat
    11401 gacaattact cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg
    11461 tttagtcatt ttgaatggtg aatcattagg tcaagtggct tcacaaactt tgcaaagtat
    11521 ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga caatggataa
    11581 acttgaaatc attgaagtag cagaacagat cgatacattt aatctttcta tccagccttt
    11641 tgaagattgc tgtacgattt ttgctcctcc acagccaaaa acacgccctc aactggaaaa
    11701 ggtccatcaa tatgaagagc gtttagcaat agatgaaatg atccaacgag cactagctgg
    11761 cttgaagatg gaagagatac aagcagaatc gtctaatgaa aaaactgaag aattttcaga
    11821 attgctataa gaggttatga aaatagtgtt ttgaccagag tattagagaa acaaaacgaa
    11881 aaaatagctc catgtatttt accgtttttg aactaatgtc gaagattagc tgattgaata
    11941 ccatttatca agaagttatg gaaaagccgg ttctttttta ctaactgaaa aataaaattt
    12001 tatttagaga tcaagctaca gttgataaat gatataaaaa agagaatttc ccatgaaata
    12061 gcgaggttgt gacagaagtg ggcagcttca agaaataaga aggaatttac gaaaattgct
    12121 tttcaaattt ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg gaacaccgtg
    12181 tatcagaggt tg
//
LOCUS       DAFCLG010000074        12117 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFCLG010000074 DAFCLG010000000
VERSION     DAFCLG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.074"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(420..1001)
                     /locus_tag="LND56_001873"
     CDS             complement(420..1001)
                     /locus_tag="LND56_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6743790.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1184..1810)
                     /locus_tag="LND56_001874"
     CDS             complement(1184..1810)
                     /locus_tag="LND56_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6743791.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1832..3163)
                     /locus_tag="LND56_001875"
     CDS             complement(1832..3163)
                     /locus_tag="LND56_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBK6743792.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3318..3719)
                     /locus_tag="LND56_001876"
     CDS             complement(3318..3719)
                     /locus_tag="LND56_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6743793.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3805..4011)
                     /locus_tag="LND56_001877"
     CDS             complement(3805..4011)
                     /locus_tag="LND56_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743794.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4106..5608)
                     /locus_tag="LND56_001878"
     CDS             complement(4106..5608)
                     /locus_tag="LND56_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBK6743795.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5626..6081)
                     /gene="argR"
                     /locus_tag="LND56_001879"
     CDS             complement(5626..6081)
                     /gene="argR"
                     /locus_tag="LND56_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6743796.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6179..6796)
                     /locus_tag="LND56_001880"
     CDS             complement(6179..6796)
                     /locus_tag="LND56_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK6743797.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            7017..8390
                     /locus_tag="LND56_001881"
     CDS             7017..8390
                     /locus_tag="LND56_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6743798.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8465..9577
                     /locus_tag="LND56_001882"
     CDS             8465..9577
                     /locus_tag="LND56_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK6743799.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9649..11286)
                     /locus_tag="LND56_001883"
     CDS             complement(9649..11286)
                     /locus_tag="LND56_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK6743800.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11454..12062)
                     /locus_tag="LND56_001884"
     CDS             complement(11454..12062)
                     /locus_tag="LND56_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6743801.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3204 a         2562 c         2181 g         4170 t
ORIGIN      
        1 aatttttctt aatactcgaa actaagcgac tttttcacaa cctcttcctt aaacggtgtt
       61 caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgagc agctattttt
      121 atttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc cactaaacga
      181 tgttcaatca gctaaccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
      241 tgccgtttgt ttctctaata ctcaggtcaa agcgctgatt tcacaacctc taatacacgg
      301 tgttccagaa gtagcttctt cgaaaaataa gccgaaattt cacaaaaatt tgagaagcaa
      361 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctaattat
      421 taaagaacct tttgcgaagt taacaatctt tctttgatca attgaaaatt ctgcctaagt
      481 agtgatagat cctgtgtgac tttcaacaat aagatacttc cctggatttc agcaatcaac
      541 gctttggctt gacggtctgc ttcttcatcg gacagttttt cttcagaaag tgccaggata
      601 ttttgtttcg tatacttgac ccattgattg actaattcta gtagcaacag acggtattct
      661 tcatccactg tactcaactc aataatcaaa ttacctacag gacagccatg ataagtgatc
      721 tgtgtctcat gatcagcgaa aatccagtcc aacatttctt ctaatgcttc ttctggctgt
      781 tttccagcat ttaagatgtc ttcgaacaat tctgtctgcc atacgtccat atgcgcatgg
      841 atcaccgcta gacaaacttc tttttttgaa gaaaagtaat aataaaattg accttttcct
      901 gtatcagaag ctgctagaat atctcggatt gacgtttcat gatagccttt tttataaata
      961 agatctttgg tcgcttccaa gatcttttcc tttgttttca atgatattct cctttttcat
     1021 ttctcatagt cctatattat acagtaaatt catcattttt tcttcttaat cgtttaaaaa
     1081 agactgtgcc aaattatctg acacagccct cactataaaa ctgttctcat atagctagat
     1141 gttcattgat tcaagaactg cgaggacaaa agcaattcct atcttatgat agttgctcta
     1201 atacttcctc tgttgtcatc acttgatctg caataaagcc aaagttcgtc aacgctgcat
     1261 ccattgcttc ttggccagga gcaccaattg catctgtcgc taccacaaca ttgaatccac
     1321 tctctaccaa ttcacgcata tgggaatcgc tacacaagtt tccgttcatt ccaccaagaa
     1381 ttactgtgtc gattccattt tttctcaatt ggagcgccaa atcattgctt tctggtccaa
     1441 agattttatg aggagagctg atgatcgtat tttcatccaa ataaggtttc aattcttcga
     1501 taaaatcggc tcctgaacct tcttccactg attcatattg actttttcta gcaaacatct
     1561 gctgctcaag catcatatgt tctcctgcac cgccaaattg ccatttatgg tcatgtgggt
     1621 aaaaataatg aggagaaata aagagtttgt atcctttttc ttttgcttgt tttaccattt
     1681 caatcatatt ttcgatcgta ttgtattttt ctaagatatc ttttgtcaaa ccaaagccac
     1741 cgcctgctgg tttcaagaac tcgacttgcg ggtcagtgag aactactgct gtatttttat
     1801 tattgaacat ggtggattct ccattcgtta tttattttaa tacatcattt agagcttcac
     1861 tcattgtcgg atgagaatag atttgatctc tcaataacgt ataaggaagt tttccattca
     1921 tagctagcga aatcaagttg atgacttcat gagaatcttc cgcatagata cttgccccta
     1981 gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac acctttagga tcttctaata
     2041 cttgtgcttt agggacaccg cttgtcatat acttgaataa tttgtaattg acgccttgtg
     2101 ctttggcttg tttttctgtt aaacctacat tggataaagt cggagtgata aatacactgt
     2161 aaggcacagc tttccggtta ttcgttgttc gtttattctc gcccttcaac tgatccataa
     2221 tgattcgata atcatctaga gagatataag tgaactgcaa tccgcctttg acgtctccaa
     2281 tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc cacagcgatt gctccgcgat
     2341 ctgttagttt aacatctgta ttttctaatc caagtgatgc tgtacttggt tttcttccag
     2401 ttgcagccaa aacttttgac gcttggattg ttacttcttg gccatcttct gtaaaggtga
     2461 cggcagctgt tgattcttgg tctgctactt gatctacaga tacacctaga tggaattcga
     2521 ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat atcttcatct tcacgtggaa
     2581 gaaattcaga atgggcatca agaacgacaa ctttagatcc gtattcattg aacattgatg
     2641 caaattccaa gccaatatat ccagccccga tgatcactaa tttttccggt aattgttcct
     2701 gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt attcaatcct ttgattggta
     2761 aaatgattgg ttcagaacca gtattgatga aaatccgttc gccttcaaca atcacttttt
     2821 caccattatt cgtcacttcg atttgatggt tagaaagaaa acgtgctgta ccgtccatca
     2881 ctgtcccagt tgcctcatcg ctgatcatat gataattttt ggcgcgaagc atgcccgtca
     2941 atttttcttt ccgagcaaca gcctctgtaa agtcgattcc tctttctcca ttgaaaatca
     3001 atgatttaga aggaatacag ccgatgttga tacatgttcc gccatacata cgtgttgact
     3061 tttcaacgac taaaaccgat tcaccttttg aagctaacgc cttcgctaaa gtcttaccgc
     3121 cctttccaaa accgatcaca atattttgat atttttccat catttttccc tcgcttcttt
     3181 tttacctacc gattggtaca aaaatatgat aaccgataat tgtctattct ccaaatcatc
     3241 tgctttattt gtcaaaaaaa accagacaaa aagcggtcct ttcacgtttt tagtctggtt
     3301 cgatttcctt tattcttcta aacaatcgtt acttctacat cgatatttcc tctagttgct
     3361 ttggagtaag ggcagaattc atgcgcatct tctgcaattt tttgggcttc ttcttcgctg
     3421 atgccatcga tatgcacttc taatactaca ccgatctgat agccgccatc atctaaatta
     3481 taaagagaga cacgtgcttt tactgtggac tctgctgtca ttcctgcttc tttgatcata
     3541 tgttgtaccg ccccattgaa acaagaagca taacctgctg caaataattg ttctggattt
     3601 gttccttctg catggattcc tggaggagtg attttcatat gcatcgagcc atttggtgcc
     3661 tcgacttctc cattgcgccc gccatggttg atcattgctg tttcgtatgc ttttttcatt
     3721 gtactgctcc tttcgattga ccatttgcta cttggctata attatagtgt atgacaagta
     3781 aggcgctttc tacaaaaatt ctgcttattc gaataccaga atgccgtctt ctactatttt
     3841 tccaaaaaac aaaaaaatac tactcgtgaa caacagcgtc atatttgttc caaaataaat
     3901 agcaatcttt tctacataat cttttatatg attgatttta agatacatat ataccagaaa
     3961 gttgacccca taaaccaata ctgttaaaaa aataaccata ttcgttacca ttttttcctc
     4021 cttttgctgg tctccctacc agcactcgat ttaaccatac cacttttttt gccgtccaag
     4081 caactagcct ttcaaaaaga ctggtttatt ctgctgttaa tgattcaaga tcagaaaaat
     4141 gtccgaataa ttttgctaat tttactaacg ccaacttgtt ctttccgaat tcatcatttt
     4201 ctatctgtaa tactagtaac ggctcatgta agcttaaacg gatcaaaaac cagccgtttc
     4261 cgtatggctc ttctagatca tatcgaatac cttcttcatt ttccgggtcg aagttcatcc
     4321 ctttttcttc ttccaacagt cgcttcattt cttctaatat cttgtttcct tgttcgcgtg
     4381 gttcatcgct gaacaatttt aatcggattt cttgtacttc tacgggctgt ttcaactctt
     4441 tgatttgttc atctaatgtg ataccttttc tttttaattc tggcaaaagc atcaggatct
     4501 ttgcgatgac atatgcacca tcatccagaa aatagttttc cttgaatgca gcgtgtccgc
     4561 ttgtttcgat ggctagctgt gtatctttcc caagacggtt aagttctaat gctttattga
     4621 ttacatttcg atagccggaa atataacgaa cttggatgcc tcctttttct tcaatgaatc
     4681 tttgtagatg agaagaagta ggggaattcg tcacgattct tgttcctgga tgctctttta
     4741 atacgattgt acttaatact gcgatcaaat tatttcgatt gatcactgtt ccagaagcac
     4801 tgacaactgc agaacgatcc acatctgtat cgaaaatgat cccaagatct gcttcatttg
     4861 ctaaaacagc ctgcttgata ctttccatcg cttctttatt atcaggattt gggatatgat
     4921 taggaaatcg gccatctggt tccaaaaatt gggaacctgt tgaatccgca cctaagctat
     4981 tcaatacttt ttcagcaaag aatccaccag caccatttcc ggcatcaaca atgattttcc
     5041 aaccatttag tggtttgctt tctagtgttc caatcccctt tttaattttt gtgactaaat
     5101 cttgcgcata aggtgtcaaa aggtctgctt tttctacgct tccagtttgt tcgcttttta
     5161 ttttttgctg ctgtgatagg ataaaagcaa tatcttcttt ttctgctcct ccttctgagg
     5221 taaagaattt tattccatta taataatagg gaagatggct agcagtcagc ataaccgcag
     5281 catcgcacga gaactgttca aattgagtgg acataaacat agcaggcgta gtagctaatt
     5341 cgaaatcgat gacatgtatc ccttgttctt tcagactttg gatcaacgcc tctttcagtg
     5401 ctggtccgct tatcctactg tcatgaccaa tcccaatcgt ccatttttta tttggatcgg
     5461 ttgatttctt ctttttcttt atccagtttg ctagtcccca gccgatcttt tttaccatgt
     5521 ctgtcgtcaa gtttacttca tatccttcaa ctggtaatgc gattcctcga atatccgatc
     5581 cattttgcag cgctttcata tctttcatat aaaagtcctc ctcatttatt ccctcatttt
     5641 ttccagcttt tctgcaaatc gctgtgcttc ttcttctgag cggcaaataa taatgaatgt
     5701 atctacaccg gccatcgttc cgacaacttc agggtaagcc acttcatcta gccaattcgt
     5761 taccacatcg gcaccatttc gttcagtcaa tacgataacc atgaactgaa cagactggat
     5821 acgcaaaacc tcgcgtctaa ttgctgtacg caaacgttct tcatttaaag aaacagatgg
     5881 ctggttaaat aatgtatagc gtactatttt gttttcattt tgacttttta ctaacttcat
     5941 ttctcgaatg tctcgagaaa tcgttgcttg agtcacatcg gcgcctgctt catgcagccg
     6001 cgcaagcagc tcttcctgtg tcccaatagg ttgttctgta atgatctggc ggatcagtgc
     6061 ttgtcgctgt ttctttttca tattagacac ctctcaatgt tgattatagc aaaagataag
     6121 taatatttct tcatattttg gtatttttta cttttatttt ttgttttcat tcaggttgct
     6181 aacctttttc tattttaaca cgcaaaatcg tcttcatttt ttcaggtgct gattttcttg
     6241 gatcgctgat gtaaatctct ttgtgttctt tagaagctct ttcatagcct tcttttgcga
     6301 tgaatgcttc taatttctca aacgttgctg gttcttcggc atagggcccg acgtgtaaga
     6361 tttgggcaca aagaccttct tccatctttt tgaatactac ttgttctttc aagtcttccg
     6421 gaagtttgct agaacgctga atggcttctt gtgctatcgc ttctgtaaca aagtcaggct
     6481 gtcggatcat caattgataa gaaaaatgtt cttttttcat cactggttca tgcaaatact
     6541 tttcttgcag tccccattgt ccttccaatg gatagacagt atacatctga taatcaggaa
     6601 tcagccaatt ttttttaggc gacatgcgta ttgcatagct gactgcatat agacaggcta
     6661 ctcttcgttt aaagtcttcc tgattcgggt cgccgatccc tctgatgcta aagaatgatt
     6721 ttttaggaac ctctagtatt tgcgggcttt ttttcatact atagctcttg tctttacgcc
     6781 attcaatttt ttccattttt cttcctccgc gaaattccat ttagttattt attatcatac
     6841 ctctttttga gtgacttggc taatcgtgtt gacactattt ttcagtttgt gctagaataa
     6901 gtgaagtaaa taaatcgtac agataacacg tttcggcgtg tcgagagaga ggcggtttct
     6961 atttttataa tagaaatcac tctctttttt tacgaaattt tttagaggtg atgtaaatga
     7021 caaaacaaaa acagctgctt ttagtacctg cagtaattgc aacagggatg atgtcttttg
     7081 cgggcgtatt gatcgagacg gccatgaatg taactttccc aactcttatc agacagtttg
     7141 atttaacgac tgcacaagtc caatgggtaa caacgattta tcttttgatg atttcgatca
     7201 ttgttccgct gtctacttat ttgaatcgca attttagtct caaaaaactc tttttagctt
     7261 caaatctgct tttcttggca ggtataatga tcgatttctt ctctcctacc ttcatggtcc
     7321 tattgtttgg tcgattgctc caaggtgcag cgacaggtat cgctcttccg ttgatgttcc
     7381 atattatctt gacatttgcc cccctcaatc gacgaggcgc gatgatgggt gtcggtactt
     7441 tgacaacggc cattgctcca gcaattggcc ccacttatgg aggcattatg acttcacatt
     7501 ttacatggaa tcatatcttc ctgtttcttg tgcccgtttt gattctttct ttgattatcg
     7561 gctggtttgc tattcccgag atggcagtac aaaaaagtgg ttatttggat tggataagcg
     7621 ttttaggaat ttgtcttttg tttagtggaa gtctaacatt tttgagtacg ttgggaacag
     7681 tttccagctg gattgcttta atcatcggcg gtattggctg gttccttttt tactatcgaa
     7741 gtaaaaaagt ggagcatcca ctgatccgac tgtctattct ttcaaataaa acattccgct
     7801 tgtttttgat tggcttctta gtttatcagt ttttattact tggtgtatct tttgtcttgc
     7861 cgaatttcct tcagatcgtt caaggaagta gtccttttgt cgctggatta gcgatgcttc
     7921 ccggcgcagc ggttggtgct ttgctttctc ctttttccgg gaaaatgctg gatcaatatg
     7981 gtccgaagaa gccaattttt gctgggctga ttctttcttt gattggttgg ctagcattat
     8041 tcctactcat aggacacgct tcagtgggtg tattgattgc ttgtcatgtg ttttatatga
     8101 tcggtatcgg attatcttac agcaatatga tgaccaccgg aatgaatgca ctgaatgaag
     8161 aactacaagg agacgggaac gcaatgttca ataccttgca gcagttttct ggcgctgtgg
     8221 ctacttcatt agtagcggtc attatcaatt acgtccagca tcatacttcc cataactatg
     8281 aagtgtctac tacattagga tcaaaagcag cattaggcgt cttgttgctg ttgcttctcg
     8341 taagctttgc tcgattcgcc tattatctgt tctttgctaa aaaagcgtag attccatata
     8401 cggcaggtgt tgcatctgcc gtttttttgt gtgaaaatgg ttgtttgtaa aaggaggctt
     8461 ttttatggaa aaaattgcca ttatcggtgg cgggatcatt ggtatgaccc tagccagtta
     8521 cttagataca tcaaaattca atgttgcttt atttgatgaa gggacaggac aggcaactaa
     8581 agctagcgct gggattatct ccccttggtt gtcgaaacgc agaaacaaac aatggtatcg
     8641 tctggctaaa gacggcgctg cttttttccc taaacttata aaagatttcc aacttgatca
     8701 aacgatttac gcacaatcag ggacactttt gcttcgacct gaaaaagatt tagctgaatt
     8761 ggctgttctt gccgagaaac gaaaagaaga agcgccagaa attggaaaca tcgaatggct
     8821 ttcacctgaa cagacatctc aagcacttcc gttattgaaa ccagcagttt cactgcgtat
     8881 cagtggtggt ggtcgtctgg atggaaaagc atatttagaa catcttggtc agcgtttagc
     8941 acaaaaacaa atggagcaga tccaggcaaa agtcactttg cataagaaga gcgattcttg
     9001 gctcatcaag ggaccgtttg gagaatacac agctgatcgt gtaattttga cacctggccc
     9061 ggcacttaag cctttattac aagaattagg ctatgctgca gatatacgtc cgcaaaaagg
     9121 gcagctctta gcatttcaga catcttataa gatgagtcaa gactggcctg ttgctatgct
     9181 tgacggagaa gctgatttca ttcctttcat ggatggaaag atccttttag gtgctaccca
     9241 tgaaaacgat caaggctggg atttggctcc tactagcgaa gcttataagg gattgactaa
     9301 gagtacagaa cagttcttga ctgatccaga tcagataaaa aactggccat acgagttccg
     9361 tgtaggtacc cgtgcttata cgtcagattt ttccccattt ttcggcgtac tgccagatga
     9421 tccaagctta ctggctgcta gcggtctagg ctcttctggc ttgaccacag gtccttatat
     9481 cggctatcta ttagccaact tcttaaatac tggtcattgg cctacggaag attatcaaaa
     9541 ggacatctcc acctatatta cgaaaaatcc atcatagaaa aacagctttt tcaagtaagg
     9601 atcccgagcg atattgcgca tggattactt cttgaaaaag ctgttatttt agttcaattt
     9661 ctgaatcaaa ttctccaaac tttcttttgc ttcatctggc aagagatctt ttcctatctg
     9721 agcttcaatg aatcgtaaac gaatatcaag tcgttgtttc accagatttt tataggacgt
     9781 atcctcaaaa gaaacaggaa aatctggtat aggtaaatag tggatgtctt caaatggacc
     9841 aaacgacttg aattctgcgg tttgttgcac gattctttca acgattgaca atgtcgtttc
     9901 aggtttgatt tttttatcat cgaaatatcc agtattcagg acatagcagg ctgtttgctg
     9961 tttcaaaaac aactctcgaa aagcttgata gtcttcccta agaggataga cacggaaagg
    10021 attagcaaat ggttcaatga ctagctcatc tagctgtctt tttgtagtga tattttccgc
    10081 gtctgatcgt tttgttgcta atgttgcacc aaaagacgct gctaaaactg ggtcattgat
    10141 cttgataatt ggcggaagcg aatcatcttt catgatccaa aaaacagcat cgatcttttc
    10201 gctaagatga tcgactcggt cgggtgtcgc ataacgtgat ttgactgtcc gcccatttcc
    10261 gtttcgtata tctcctgtta ctaggacttt ttctccttta cttgtacatg tcacgcccac
    10321 attctgacaa gtaaggaaag aagaaatggc cggatcatca gacggatagt cttgtgtttt
    10381 atcaaagtaa gaaggttcca aggcagtagc aaaaccattt tttcgagaaa taacgaaagc
    10441 atcgtcatgc aagatcgaaa cagagtgacc ttccgtatgt tttgctaagg taatcgttga
    10501 ttttccagaa ccggacaacc caaatacagc catcgtatag gtctggtcat tactagcata
    10561 ttgtttcatc ccaccgtggc aggcaataaa gccattgcga tttcccaatg cccaagcaag
    10621 cgtcaaagtc gcttttttta attctccaaa gtaacgtaat cctagcacaa ccgctgtatt
    10681 atggagagca tcaaacaaaa tgagtccatt tggaaatgtt tcatgtgtcc attcgggatc
    10741 acaaaagata taaagatctc cctcatcaaa ggaaacagac ttttgataag tctctaagat
    10801 cttcgaattc gctggctgaa aattcagcaa ataagaatac aaattgttct caaatccttc
    10861 tggaatcagc aaatgacttt gcacagaaaa ttcttcagat aggccaacga ttacttttgt
    10921 atggtaaaac tggcgcttgc gactttgata cacagcttct cttaaaattt tctcgtattt
    10981 gctttgttcc ttgtctaagt cctcaaagat ccgccgagca cttgctgttc ttcccacaat
    11041 gcttccatca ttgaatacta gtgtcctaac atcagctggt aaatctagtt cttcagcatg
    11101 tgagattgtt aaatctgtga tgatcgttcc tggactccct tttgctagtt gataagctgt
    11161 cttcctatca aaaacttcat gaacattatt accgtaaaat gtcgtttcta caatcgatcg
    11221 aatactggat aacagtggat ttccacttgt gatttcgttt aattcgagac tctcttttgt
    11281 actcatggct cctcctcatt ccctaaacaa aaaaacgttt tctttattat acattattcg
    11341 agtttttatt tgtactgctg cgctgtggga agcaaacgag ttatttttca cacaaaaaaa
    11401 ccgagaactt gaataaacaa gtttctcggt ttcatcattt tccttttatc ggcttagtac
    11461 cagccgtttg ctaaccagaa tgttttagca gcatcccatg aaccataacg agatgttaca
    11521 tattgttctg ctacacgttc ttgattggca gctgaatagt cgccgtttaa gtatgaagca
    11581 tctaattgat aacggccgat gtaacgtccg ttagtagctg tgtatgaacc gcctgattct
    11641 ttttgtgcga tccattcttt tgcagagctt gaagttggcg ctgttgcagc agctggggca
    11701 gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg tatgttcttg cgttgctgtt
    11761 tcagttgtag atgctgtatt ttctgttgtt ttgttttctg ttgttgctga agaatcattt
    11821 gaagttggga ttgttaattg ttcaccaaca taaatgcggt tgatgtttgc gatcgaattt
    11881 ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa atttttgtga gatcttagat
    11941 aatgaatcac ctgattttac tgtataaact tcatcagcgt gagcatttgt agttcctaca
    12001 aacattgcag tagcaccagc agcaaaagtt gtacctaaaa caattttctt aaatgaattc
    12061 atattaaact cctttttttc tccgtttttt attctttcga cgctattaat tttatca
//
LOCUS       DAFCLG010000075        12099 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFCLG010000075 DAFCLG010000000
VERSION     DAFCLG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.075"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..260
                     /locus_tag="LND56_001885"
     CDS             51..260
                     /locus_tag="LND56_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743802.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="LND56_001886"
                     /pseudo
     CDS             467..667
                     /locus_tag="LND56_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="LND56_001887"
     CDS             complement(1379..1984)
                     /locus_tag="LND56_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6743803.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="LND56_001888"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="LND56_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="LND56_001889"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="LND56_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="LND56_001890"
     CDS             3019..3990
                     /locus_tag="LND56_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6743804.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="LND56_001891"
     CDS             4130..5443
                     /locus_tag="LND56_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6743805.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="LND56_001892"
     CDS             5486..6352
                     /locus_tag="LND56_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6743806.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="LND56_001893"
     CDS             6349..7197
                     /locus_tag="LND56_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6743807.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="LND56_001894"
     CDS             7211..8683
                     /locus_tag="LND56_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBK6743808.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="LND56_001895"
     CDS             8700..9572
                     /locus_tag="LND56_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6743809.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9808..11190)
                     /locus_tag="LND56_001896"
                     /pseudo
     CDS             complement(9808..11190)
                     /locus_tag="LND56_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11652..11933
                     /locus_tag="LND56_001897"
                     /pseudo
     CDS             11652..11933
                     /locus_tag="LND56_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            12007..>12099
                     /locus_tag="LND56_001898"
     CDS             12007..>12099
                     /locus_tag="LND56_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6743810.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREV"
BASE COUNT         3762 a         2061 c         2433 g         3843 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aagttcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggctacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     9841 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     9901 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     9961 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
    10021 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
    10081 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
    10141 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
    10201 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
    10261 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
    10321 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
    10381 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
    10441 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
    10501 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
    10561 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
    10621 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
    10681 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
    10741 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
    10801 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
    10861 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
    10921 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
    10981 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
    11041 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
    11101 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
    11161 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
    11221 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
    11281 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
    11341 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
    11401 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
    11461 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
    11521 ggatatcatt tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa
    11581 atttaatata taatattcgt tgtaagggct tataattttt tgttaatgaa caagatagga
    11641 agaaacattt aatggacagt tttcagagat tgtcactagt aaatcaattt gagttattgg
    11701 ctaaatttga ggaccaaaac agatcttatt atgaaagaaa aatcgaaata ttgagagaag
    11761 gttacgagta tcattatgat gatgaaattt ggagtgatct ttctgagcca tttccgaaag
    11821 aagattctcg atttgtatta gatgtattga acatgtatcg agatatcaat tttagtaaat
    11881 caaagctaaa cttagatgaa aaccaaatta ctaattcata ctatactcat ttcggttctg
    11941 ttgcaaagtt ttccaaaaaa tctattttag tgtaaaattg agaaaaaaga cagagaggac
    12001 agagtaatga atcattttaa aggcaaacaa ttcaaaaaag acgtcattat tgtcgctgtt
    12061 ggttactacc tgcgttacaa tctaagctat cgtgaagtt
//
LOCUS       DAFCLG010000076        11885 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFCLG010000076 DAFCLG010000000
VERSION     DAFCLG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.076"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..317
                     /gene="yaaA"
                     /locus_tag="LND56_001899"
     CDS             75..317
                     /gene="yaaA"
                     /locus_tag="LND56_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBK6743811.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            304..1428
                     /gene="recF"
                     /locus_tag="LND56_001900"
     CDS             304..1428
                     /gene="recF"
                     /locus_tag="LND56_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK6743812.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1425..3371
                     /gene="gyrB"
                     /locus_tag="LND56_001901"
     CDS             1425..3371
                     /gene="gyrB"
                     /locus_tag="LND56_001901"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK6743813.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3391..5862
                     /gene="gyrA"
                     /locus_tag="LND56_001902"
     CDS             3391..5862
                     /gene="gyrA"
                     /locus_tag="LND56_001902"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBK6743814.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6098..6397
                     /locus_tag="LND56_001903"
     CDS             6098..6397
                     /locus_tag="LND56_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK6743815.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6447..6959
                     /gene="ssb"
                     /locus_tag="LND56_001904"
     CDS             6447..6959
                     /gene="ssb"
                     /locus_tag="LND56_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6743816.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6985..7221
                     /locus_tag="LND56_001905"
     CDS             6985..7221
                     /locus_tag="LND56_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK6743817.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7364..9337
                     /locus_tag="LND56_001906"
     CDS             7364..9337
                     /locus_tag="LND56_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBK6743818.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9343..9795
                     /gene="rplI"
                     /locus_tag="LND56_001907"
     CDS             9343..9795
                     /gene="rplI"
                     /locus_tag="LND56_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK6743819.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10069..11436
                     /gene="dnaB"
                     /locus_tag="LND56_001908"
     CDS             10069..11436
                     /gene="dnaB"
                     /locus_tag="LND56_001908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK6743820.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3977 a         2156 c         2637 g         3115 t
ORIGIN      
        1 ggataaattt gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa
       61 atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc
      121 aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag
      181 aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg
      241 gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg
      301 ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc
      361 gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt
      421 ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa
      481 ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc
      541 actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc
      601 gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa
      661 gatctttctt tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta
      721 ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa
      781 agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat
      841 attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc
      901 gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag
      961 cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa
     1021 atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga
     1081 acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg
     1141 cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa
     1201 atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg
     1261 agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa
     1321 acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag
     1381 attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa
     1441 gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg
     1501 aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc
     1561 accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga
     1621 aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga
     1681 tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt
     1741 tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag
     1801 taggatcttc tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag
     1861 gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg
     1921 aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag
     1981 agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga
     2041 accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat
     2101 accattatga cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga
     2161 tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta
     2221 tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt
     2281 atgaaggcgg aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt
     2341 atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc
     2401 gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa
     2461 caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac
     2521 atttcatgaa attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat
     2581 tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag
     2641 gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg
     2701 aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc
     2761 gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag
     2821 caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta
     2881 cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga
     2941 ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt
     3001 acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg
     3061 taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga
     3121 aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac
     3181 ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga
     3241 tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg
     3301 gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact
     3361 tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat
     3421 gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt
     3481 gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta
     3541 tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc
     3601 gtcggagatg tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg
     3661 gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt
     3721 ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa
     3781 atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat
     3841 gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac
     3901 ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag
     3961 gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg
     4021 gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc
     4081 cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact
     4141 gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa
     4201 gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca
     4261 gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac
     4321 gccagcgcat ctgttatctt gaataactta tataaattga catccttgca aaattcattt
     4381 ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt
     4441 cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa
     4501 gaaaaagcag aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc
     4561 gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg
     4621 atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc
     4681 cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg
     4741 attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat
     4801 gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt
     4861 gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg
     4921 acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt
     4981 ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct
     5041 tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa
     5101 ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta
     5161 ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa
     5221 ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct
     5281 tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc
     5341 gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac
     5401 tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga
     5461 ggaatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa
     5521 gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca
     5581 gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca
     5641 ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt
     5701 gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta
     5761 cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa
     5821 gctgcagaac aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg
     5881 taagctagca agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag
     5941 ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga
     6001 gtgtataatc agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga
     6061 acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat
     6121 catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt
     6181 cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg
     6241 ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc
     6301 ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga
     6361 tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata
     6421 gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga
     6481 cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg
     6541 ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg
     6601 tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag
     6661 gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg
     6721 tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc
     6781 gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca
     6841 ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt
     6901 ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa
     6961 cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt
     7021 aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta
     7081 ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc
     7141 gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta
     7201 cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc
     7261 tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga
     7321 aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa
     7381 tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt
     7441 cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg
     7501 tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca
     7561 gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg
     7621 agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa
     7681 aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa
     7741 agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac
     7801 agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca
     7861 gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag atgccattga
     7921 taaattggat gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga
     7981 ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt
     8041 agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt
     8101 tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg
     8161 ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt
     8221 ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta
     8281 tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac
     8341 cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc
     8401 ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa
     8461 aaaagcttac atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga
     8521 agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga
     8581 gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat
     8641 ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg
     8701 ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc
     8761 agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt
     8821 tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac
     8881 aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt
     8941 agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc
     9001 tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta
     9061 taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc
     9121 agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg
     9181 cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc
     9241 agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat
     9301 caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta
     9361 caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca
     9421 caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag
     9481 ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa
     9541 aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg
     9601 gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa
     9661 caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta
     9721 ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat
     9781 gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga
     9841 aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca
     9901 aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa
     9961 cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc
    10021 tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg
    10081 tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt
    10141 tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat
    10201 cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg
    10261 attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt
    10321 ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac
    10381 tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga
    10441 atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa
    10501 cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa
    10561 gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca
    10621 ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa
    10681 ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct
    10741 caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca
    10801 gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt
    10861 acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct
    10921 cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa
    10981 tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag
    11041 ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa
    11101 ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt
    11161 ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca
    11221 attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa
    11281 ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc
    11341 gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat
    11401 aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca
    11461 atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta
    11521 atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa
    11581 taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga
    11641 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt
    11701 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg
    11761 agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct
    11821 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa
    11881 tatga
//
LOCUS       DAFCLG010000077        11838 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFCLG010000077 DAFCLG010000000
VERSION     DAFCLG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.077"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(560..1600)
                     /locus_tag="LND56_001909"
     CDS             complement(560..1600)
                     /locus_tag="LND56_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6743821.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="LND56_001910"
     CDS             complement(1949..2275)
                     /locus_tag="LND56_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743822.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="LND56_001911"
     CDS             complement(2262..3065)
                     /locus_tag="LND56_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6743823.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="LND56_001912"
     CDS             complement(3418..3708)
                     /locus_tag="LND56_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK6743824.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="LND56_001913"
     CDS             complement(3844..4023)
                     /locus_tag="LND56_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743825.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="LND56_001914"
     CDS             complement(4020..4370)
                     /locus_tag="LND56_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743826.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="LND56_001915"
     CDS             complement(4363..5688)
                     /locus_tag="LND56_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK6743827.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="LND56_001916"
     CDS             6138..6752
                     /locus_tag="LND56_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6743828.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7159)
                     /locus_tag="LND56_001917"
                     /pseudo
     CDS             complement(6917..7159)
                     /locus_tag="LND56_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7363..8682)
                     /locus_tag="LND56_001918"
     CDS             complement(7363..8682)
                     /locus_tag="LND56_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBK6743829.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8837..9424)
                     /locus_tag="LND56_001919"
                     /pseudo
     CDS             complement(8837..9424)
                     /locus_tag="LND56_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10062..10319)
                     /locus_tag="LND56_001920"
     CDS             complement(10062..10319)
                     /locus_tag="LND56_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6743830.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10312..10581)
                     /locus_tag="LND56_001921"
     CDS             complement(10312..10581)
                     /locus_tag="LND56_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6743831.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10712..10891)
                     /locus_tag="LND56_001922"
     CDS             complement(10712..10891)
                     /locus_tag="LND56_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743832.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(11272..>11838)
                     /locus_tag="LND56_001923"
     CDS             complement(11272..>11838)
                     /locus_tag="LND56_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743833.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3603 a         2163 c         1789 g         4283 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatcc ctgaataatt catagcttta attctttggc acattttatt
     7201 gaaatatgta tcactaatat ccctatcaac tgattttgac taaaaagtta cttcaaacat
     7261 tcactgttca aaaatattga ggaataaatt ttgggtgtaa ggcattttga gcatacttct
     7321 ccctggataa cttcccagta aaaaaatcgt tagattgttg gattagatcc attgactggc
     7381 tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga tacacatgat aactagataa
     7441 tttgagatag actcgtctac ccgtttggac taatttccca gcaactttca gaaacttcaa
     7501 tcgaatagaa tgaatggtca ttcccttttg gacttcttca aagccaatcg ttcttaagaa
     7561 gttgactaag ttgtaagcta tcaagctgag catcattcgg acatgattct ccaaaaagcg
     7621 aggactgtct gttttgtcaa agtaaaagcc agctttcgct tctttaatga agttctccat
     7681 tgtgccacgt ttggcataga tagaaaagat ggtatcagga gaaacatttt ctgatagatt
     7741 cgtcacgata aattcatgtc gaaagagtag ttcgcccgct tcacgtgttg agcgtataca
     7801 cacgcgacga ctttgtgacc aggattgtgc ttgataagtg gtcgagaagt actgaacttc
     7861 tcgttcttcc cacttttgat tatcgccata aagtactgat ttctcagcta tttgacctag
     7921 tctacgatta ttcttcagtc gaataacata atgactcttt tttgattcac acgaatcata
     7981 cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga
     8041 gtgattatag tgctctaata aaggtgtgag aaactccttt acgccttttg atgtatattg
     8101 atttcctgaa cgtagttcag cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa
     8161 tggatggtaa ccataggttt ggtagtgtgt attataattc gtttgttctt ggtgaccaaa
     8221 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
     8281 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
     8341 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
     8401 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
     8461 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
     8521 gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc
     8581 tttcactagg acgagacccg aatcactcga taattgacca cctgtatgag aaatagtgat
     8641 atttgaattg aattttactt ggtttttgtg taagctagtc atgaaaagaa ctcctttctt
     8701 ttggttggtt tagtcacttt aaccatagca gaaaggggtt cttttttcat cacttaacgg
     8761 gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa
     8821 agtatgaatt attcaggcta tcattaattt tataaaaaaa attaaatgtt tttagcactg
     8881 ccatataaca ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca
     8941 atttattacg tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa
     9001 tattaaagta ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat
     9061 tctcaaatat tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt
     9121 cacaggctgt aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt
     9181 taaggagtat aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt
     9241 gagcttcaga agttatatct ctaattatat agttaacgga attaggaagt gtctttaact
     9301 ctaatgaaat ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg
     9361 attcgtcagc aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa
     9421 tcatttcagc aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta
     9481 tatcagaatc aataaattct atttttttat tactatatcg tcatcattat cttgaccatt
     9541 tatgtttatt gttccctgag tggggagtac ttctttcaaa ttcaagttaa atttatattc
     9601 cattacttct cctccaatat ataaaaatta cttaactgta tccaaattta atatgtaaat
     9661 aaatcattaa tttttagcta aattctatga gaagtttatt tttgttgttt ttgctaatta
     9721 actaaaacta gtacaaaaaa tttaaaatag attattaaac ctattgctta ttctattcag
     9781 tcatatatat aagcgtcatc ctttagtaac taacatccca cactacaata gtattataac
     9841 atatctattc aacttcaaat tgttgaaatt taagccattt atatttactt ttcctcaact
     9901 ttagaaatga ttttactaac cttatatccg taatacgcgt aacaaacaca ttcattaata
     9961 acaatcaaca ctaagctata ttagcttttt aaatgactag ggtaaataaa ataaggatca
    10021 aaaactttta agtttctgac cctttcctta cttccgattg gttaatagtg atcttttgca
    10081 gaataaataa atatcgtttc attttcaact ctatatatca gtctatgttc atctgtaatt
    10141 cttctggacc attttccaga taaatcatgc tttaatggct caggttttcc taatccagca
    10201 aagggggaac gatcgatatc ttttattaac ttgttaatct tttttatatt gcttttgttt
    10261 ccttgctcat gccaataaag ataatcatcc caagcatcat cagaccaagc cttaatcatc
    10321 agattcaacc tcgattaagt catgtgtttt aaatgcacct ttggagaatt gttcatctcc
    10381 tcgacgaatt ttttccatga cgtaattatt agaaagtgtt ctcaacgttt cttgcataga
    10441 atcataatct ctttttgata atacaacaac tgtatcttct acatctttac ttgttacaat
    10501 aagtgtttca gcatcctcat taacttgttt catataacta cgtaaatttt ggcggaaatt
    10561 tgaataagct actgcttcca ttcctttcac cccttattta tttctataaa acaattgtac
    10621 attatattgt acaattaagc aaatgattaa tattctaact tcttataatt aaaagtgcta
    10681 tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt acctcaaatc
    10741 ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg gattcacttt
    10801 tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg
    10861 gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat cgaaataacc
    10921 ctatgaaaaa tatggctgca atcatcttga atagtagact cacaagggct aatacaaagc
    10981 gccaaaagat caaacaaaat accagtattg ccaaaaatac tacgaagttt aaatgcacag
    11041 ctgcagcatg gcgcataagt atttcgccaa caaataggac taacaataga ggaatgattt
    11101 tattcatatg gatctccttc tttctcttag gttatcgggt gttgcccgct tatttcgcac
    11161 cacctaagcg gcgggggcgg ttggagcgat tggtgcgaaa taagaaccat tcccttagtt
    11221 actaggtttt ttgtccttat ttcacacatt ctcacttttc ggtataaatg attattgcct
    11281 ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat tttggatttc
    11341 ttctgtatcg tcgtaagtat aggtgtctaa aaaatcagtt aaagtcgtgt accagtcgtc
    11401 gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta cagcgagttc
    11461 ataatccatt cccattaaca cgatagattc cgttccacac gtcaaaaatc tagtaaatac
    11521 aaggtaataa tcgtctatcg tcgcatcttt gctgtttagc tcttccattt cttcttccat
    11581 taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt gtttcctgca
    11641 aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa cattcatgat
    11701 gtatttttct gtttctagtc gtagaagctc cgttcctttt tttaccgttt caggacgacg
    11761 tttatacacg tctaaacgat tatggctgac tgtgaccttc gtttgactcg tttcactttc
    11821 caaatacaat tgttcttc
//
LOCUS       DAFCLG010000078        11712 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFCLG010000078 DAFCLG010000000
VERSION     DAFCLG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.078"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..832)
                     /locus_tag="LND56_001924"
     CDS             complement(8..832)
                     /locus_tag="LND56_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6743834.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(805..1485)
                     /locus_tag="LND56_001925"
     CDS             complement(805..1485)
                     /locus_tag="LND56_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6743835.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1508..2446)
                     /locus_tag="LND56_001926"
     CDS             complement(1508..2446)
                     /locus_tag="LND56_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBK6743836.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2691..3554)
                     /locus_tag="LND56_001927"
     CDS             complement(2691..3554)
                     /locus_tag="LND56_001927"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK6743837.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3696..6269)
                     /gene="mprF"
                     /locus_tag="LND56_001928"
     CDS             complement(3696..6269)
                     /gene="mprF"
                     /locus_tag="LND56_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6743838.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6410..7216
                     /gene="proC"
                     /locus_tag="LND56_001929"
     CDS             6410..7216
                     /gene="proC"
                     /locus_tag="LND56_001929"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK6743839.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7295..8851)
                     /gene="rny"
                     /locus_tag="LND56_001930"
     CDS             complement(7295..8851)
                     /gene="rny"
                     /locus_tag="LND56_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBK6743840.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9262..10311)
                     /gene="recA"
                     /locus_tag="LND56_001931"
     CDS             complement(9262..10311)
                     /gene="recA"
                     /locus_tag="LND56_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK6743841.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10420..11661)
                     /locus_tag="LND56_001932"
     CDS             complement(10420..11661)
                     /locus_tag="LND56_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBK6743842.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT         3363 a         2605 c         2029 g         3715 t
ORIGIN      
        1 ttataattta gttatttttt cttctgaagg caaccaatat tcgtttgatg atatttacaa
       61 tcaggaacaa agcaataaag actaatgtga ttgttgctcc tggaggtgta tctagataaa
      121 acgagctagt tagtcctgac agcatcccta gaaatcctac acaaacactg atagcaatca
      181 cgacattgaa gcttttgcct aatcgcatac cgattgctgc gggaagaacc atgattgcgg
      241 atatcaaaag tgcaccggca atcgggatca tcacagcaat agccacacca gtaatcacat
      301 tgaacatcat cgacatcaaa tgaacgggta gcccatccac gtgggcggta tcttcatcaa
      361 aagtcagaac atacatcggc cgtttgaata aaaagaacaa tataccgatg gcaacgaata
      421 agattcctaa aaagatgact tgctcccatg taatggtcac gatcgaacca aacagatagg
      481 attggatact tgttgctgaa ttccctccgc tcaaattcat caatactaac gctagcgcca
      541 aaccaccagc cattaaaatg gcaatggaga tttctgaata ggttcggtag atcgttcgca
      601 gatactccaa tatcacagct gctaatacaa cgactagcat ggtcgtaagt gtaggattaa
      661 ggttcaaaaa gaatcctaac gctacccctg ctaaagagac atgagaaagc gtatcagcca
      721 tcagcgattg tcttctgatc actaaaaaca cacctagcat cggggcgatt ccggcaatga
      781 agatagcggc aagaaacgct cttctcatga attcatatga aagcaacgcc attgagaatc
      841 ctcctttctc accagtcgga tctgtctgtc cgcatactgt ttgatatctt catgatcatg
      901 ggtgatcatt agaactgatt tcccatggac atgcgcacta tgtcggagaa gacgataaaa
      961 ttcatttctg gattcttcat ccatacctgt tgttggttca tcaagaacaa ataagtcagg
     1021 gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt tgtccgccag acaattcacc
     1081 tattcgccga tttcgcatat cccacatccc tacagcttct aaagcacgct gtacatgttg
     1141 atgatctttt tcagagagac gcttgaacca gcggcctctt ggatatcttc ctgaacggac
     1201 taattcgatc accgtactag gaaaacctgc attgaaagat gcaacttgtt gaggaatata
     1261 accgatactt aatttttttc cttcttgatt tttctcagca atctttatca ttccttttga
     1321 aggtttcagt aatcctaacg atgctttcac caaggttgac ttggctgctc cattttctcc
     1381 agttaaaatg acaaattcac ccgcatctac atgataagag acatcttcta gtacaggttc
     1441 atcatcatag taaaaggtca aatcagatat ttcgatataa cgcaccttac aacctccatt
     1501 ctaaaactta attgatactt tcttttaaag ctttcagatt tttttccatg acagaaatat
     1561 aattttcgcc tgctttgatt tgttcattag tcaagctttc taaaggattc aacacttcta
     1621 gcttcactcc tgtttcattc gacaatgtct cagctacctt cgaagaggca ttttcttcaa
     1681 aataaatcac tttgatgttg tttttcttca caaattcttt tagctcagct aagcgacttg
     1741 gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac ttgttttaag tcgtattgat
     1801 tcgctaaata agcaaaagca gcatgctgag tgacaaatgt gcggtttgtt gcatctttta
     1861 gctcatcgga gtatttttga tccagtgctt caagcttttt gatataagca tcggcattct
     1921 tcgtaaaagt ttccttttta tctggatatt tttcgcctaa ttcttcagca atcgttctga
     1981 cttcttttat agctaatacc ggatcgagcc aaacatgtgg atcgtattca tgtgaatgac
     2041 catgcccttc ttctgattct tcatgttcgt ctgcttcttg cgattctaaa cggtcgattc
     2101 ctttagctgc ttcgatcatc aaggtctgtt tgctatccac tgaatccttt aacgaatcca
     2161 caaaaaattc catgtctgaa ctattataca cgaaaacatc tgcatcggaa atttctgctc
     2221 tttcttttgc acttggttcg aaatcatgcg gctccgttcc agcagggatc aaaagtttca
     2281 cgtttccttc atcccctacg atctcttttg taaaatcata catcggataa aaagtcgtaa
     2341 cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt atttccacat gctgcaaaca
     2401 gtaaagcacc tatcattacc gtagctgtca aaagatattt tttcatttat atgctccttt
     2461 caatgaacaa atcggaattt tacgatttat taacgtgaac taatgtatca aaataaaaaa
     2521 acacagtcaa tagaaatcca tcaatttctg atttaagcat gtatattctt gattaaatcg
     2581 taaaatggat gagttgctgc tcatatctag ccatttcact acaataaaaa actgaagaat
     2641 accattttag gattcctcag tttttcatca cctattttac ttcttatatt ttattgcagg
     2701 gttcgtacta aataaacttc ctcacaaaaa ccttttaacc cattatatac tctctgcgct
     2761 cttgaataat gttgacacaa ggcaaaaacg gttggtccgc ttccactcat caatgcagca
     2821 tctgctccgt acttgatcat acgctctttg atttgatgga tgactggatg ccgctttccc
     2881 gttatatctt ctaaactgtt tcccattttt ttgatcatct gctgataatc ttgctctcgg
     2941 atggcttcag caagtccgtg gatgtcttga tgttggatgg cttgcgtatc tacttctcta
     3001 aacactgtac gtgtagagac actcattctt ggtttgacca acacaaccca gcactgtggc
     3061 atagaaggta atacttggat ttcttcccct ttgccaccga caaatgccgt acttccgtag
     3121 acacagtaag gaacatctgt accgacttcc aaaccgattt ctgccagttc ttctaacgaa
     3181 cagcccagat tccataattg gttgatccct cttagagcag ctgcacagtc tgtacttcct
     3241 ccgcctaatc cagctgctac tggaatattc ttcttaacaa agatcttgac accggttttg
     3301 atattgtatt tattttttag caataaagca gcttgataaa cattgttccg ttcatctacc
     3361 ggtaaaaatg ctttatttgt ttctacaatg attttgtctt ctgttatttc ttcaaaaaat
     3421 agatgatctg ccaagtctac gctcgacatc accatttcca actcatgata cccatcttgc
     3481 cgtctatata atacatccag tcctaaattt atcttggctg gcgctttttc aatcaattcc
     3541 atcctctcac ccattcgaaa agtctaagtc gcaagtattc tatcataggg aaaaataaaa
     3601 gtatagctta tgtctgccta gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat
     3661 tttctcttca taatccaaac aagttctagt gattcttata attctgtgag cacttcttct
     3721 gccaaactcg gaacatttcc tttttctttt cctatcagac tgagtagctg catggtagca
     3781 aatccgaaat tacttccttt cctataagca aaataacgtc cttcccaatg atcgacatat
     3841 tttaatttct tttcataagc agactggaag gaatagaaaa aattaccata attatatacg
     3901 atatgcatga ctttttcttg aaagaaagaa aaatctgttt ctccaacatt agctaatgga
     3961 gctaagccta gattggcttc cgagtagccc attttctcgt atacaataaa cagattggct
     4021 aacagatagt cagctaattc ctcagattct ttaggtaaga cccgcaacaa atcatatccc
     4081 gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa cgactttttg atctttacgg
     4141 acaataccga caccgctaga taatacatat gatttatcaa atcgtcccac agagaagtat
     4201 ttttctcgtg caccttgaag ccatttttgt gaaacttcat ttacagcttg atgtaattct
     4261 tccggcagtt gatcgtacca agtaaaatgg tatccttctt tcatcaaacg ttgatattca
     4321 agttgctgac tcagccgaga caagtcttct gttgctagat caacaatccc tgcttcacct
     4381 actttcgtga agtgaaaacc caattcatga agcatcacta cgtagctttc tgatatctta
     4441 taaaacgcaa gctgatagcc taaacaatct gcttgtttca taaactgatt cgttgctgcc
     4501 cgcatcattt taggattgcc aactggatct cctaaaacaa agcatttgtt tcctttgatc
     4561 tgataaccaa aaacgacttg atcttggtcg tcttcttggt aataaaaata actgtaacct
     4621 gacaagcgaa gctgatggct tgtagctgtg cctccgtatg tttccaataa acggtcaaaa
     4681 cggtcttttt cgtactcact gccaagcttc gtagtatctt cagctaaata ttgataaagc
     4741 gccactaaag ctagcaatga gatgcccaaa ccaataagcc ctgagaacca cacatcatct
     4801 gaaggaaaca aaataaaatg atttcctgtt attcgaccga ctaaaaagcg tccgctgtga
     4861 tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca agctagcatc aaagattata
     4921 gctccccaag aataaacgaa ttttgttcga taaaattctt ttctggataa ccacacagct
     4981 aacagaatca agacatatac caatatcaac tgccaagata acgtacgtgt gattgtattt
     5041 aaaataccaa aagatagtaa agcaatcgta ggcagaaatg cctttttgac ttttactgaa
     5101 agtgctctag caaggccgag taataaaaag ccaaccaata gattcactgt ctgatctaaa
     5161 aaattgaatg agaacggcaa taatacttga aatagccgac tgattgcaga cagattagtg
     5221 attgttgata gaagtaccat catgatcccc gcaaaataga cagcacttac gagcacgata
     5281 tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta aaaatcgatt gattttgact
     5341 cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag gtagaacata ataaaacaac
     5401 cgataatata atagccaaac tactgctaca tcttgccgaa caggcaattg actcattcct
     5461 aagatcatca aaacgtcaaa agtccccatt ccacctggga ccatcgtcag cattcctatc
     5521 aatgtagcaa tcatgaacat aggatagatc gaaagcatcg aaatatctat tctcatcaac
     5581 ttgccgatga tcaaaaatac cgcaagggct cccgtccatt gtccaaagga tgccagtagc
     5641 agaccaagca agccttttgg ataaaactcg aagaacaatg tctttctctt tagataagca
     5701 aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa gccagatcca atagcttcga
     5761 aaaatacttt ctgactgaat aaaaaagaca tcaataaatg taagaaacga ccatacagac
     5821 aaaccagtca gcataaatag tgcgactttt gatacagtgg ctaaaaccat ttttctattt
     5881 gtctgctttc cataaaaact agcccgcaga cttgctccta ccacaccacc aaaaccggcg
     5941 agattgttga tcgtgtttgt tacccacgca gcaagaagaa actcttttct ttccattttt
     6001 ggttttcctt gtttttctaa gactctgatc gtcacccaat catacaacaa catcggagtg
     6061 acacccagca gtccagccag aatcatgagc aggatcgtca tgcggctttg ttgtcccatc
     6121 ctttgccaaa tatcttgcca actcatgcca ttagcaatat ttgcgacttg atttgccaca
     6181 aagactaaca cggagccgaa aaaaatcagt tttaaaatca gactatgttc ctttatccat
     6241 tgcagcatca agtatcgttt atttttcaca ttttcccatt cctaacttgt atacttttgt
     6301 gtcatacccc aatgttatct tatttatttt aatttttcca ctttagacct gttctttgct
     6361 tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga gaggataaca tgaaagtagg
     6421 atttatcggc acagggaata tggcacaagc tattattgat ggtttattaa aaaaaggaac
     6481 agtctcgaaa gaagaggtgt tcgtttccag tggacatttt gaaaatgcac aaaaatttgc
     6541 tgctgaaact ggcgtccatg catgcaaaag taatcaggaa gttgcatcac aatcagatat
     6601 cgttctttta gccgtgaaac caaaaatcat tgaaaaagta ttggaagaac taagagaata
     6661 tagccagaaa gtcttattca tttctattgc ttcaggaaaa tcattaacag atttagaaac
     6721 agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt
     6781 aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc acggaggaac agcttcagca
     6841 agctacgatg atttttgaag caatcggcag cgtctatcct ttagcagaat cagatttcag
     6901 tacattcatt ggccttgctg gcagctcacc cgcattcgtt tacatgctga tcgatgcgat
     6961 gggacggact ggtgtactga atggtttgtc caaacagaca gccactgaga ttgctgcaaa
     7021 agcaattatg ggttcttgtc aaaaattcct ttcaagtgat aaaaatccat gggaacttgt
     7081 agaccaagtt tcttctcctg gcggtacaac tgttgcaggt gtagttgcct tagaagaagc
     7141 aggattcatc ccagcaatca tcaaaggaat caatacgacg atcgagaaag atcttgagat
     7201 gttgaataaa aaataatgat agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt
     7261 ggcttttgcc gcggctcttc ttttgtattt tttattattt agcataatca gttgcacgag
     7321 tttcccgaat aacgattact ttgatgtgtc caggataatc cagttcttct tcgattcgtt
     7381 tacgaatatc acgtactaaa cgaacagcgt ctaaatccga gatttcttct ggcttgacca
     7441 ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga ttctacaccg gcgaagctgt
     7501 tcgaaatatt ttccagactt cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg
     7561 caccaggtct tgctgctgag agcgcatctg ctgcagctac aaggacagag ataacagaag
     7621 tagcttccac atctccatga tgagaagcga tcgcgttgat tactactggg ttttctttgt
     7681 acttggcagc taattccgca ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg
     7741 ctttcccaat atcatggagc aacccggcac gttttgctaa ttggatatct tcgccaagct
     7801 ctgctgctaa gataccagct aattttgcaa cctcgaccga atgcttcaag acgttttgtc
     7861 cataactagt tctgaatcgc agacgtccga tgatcttgat tagatctgga tgaagtgtat
     7921 gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg aatacgttca tccatttctt
     7981 tacgagattt ctcgaccatt tcttcgatac gcgctggatg gattcgtcca tcttgtatca
     8041 gtttttccaa ggtcatccgt gcgatttctc ttcggattgg atcgaatccg gaaagaacaa
     8101 ctgcttccgg tgtatcatcg atgattaaat cgataccagt cagcgtctca agcgttcgaa
     8161 tattacgacc ttctcgtcca atgatacgac ctttcatttc atcattcgga agggtaacga
     8221 ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg ttgaatcgct aatgataata
     8281 agtttttcgc tttgcgatct gcttcttctt tggcacgctg ttcagattct tttaccatta
     8341 gagttaactc atgattcagc tcttcttcag tcgatttcat gatgacatct cgtgcgtcct
     8401 cttttgataa accagcaatt ttttctagtt ctttttgttg atcttcaatc attttttcaa
     8461 cgtctttttc tcgctcttca attaattgct gttttgaact aagtgcttct tctttacttt
     8521 caagtgagtt ctcgcgtttt tctaacgagt catctttacg attcagcgtt tgttcgcgtt
     8581 gaatcaaacg attctcttga gattttaatt ctaacttact ttctctcagc tcactttcaa
     8641 cttcagaacg gtatttctga ttttcttcct tagcttctaa caatgcttct ttttttagag
     8701 tttctgcttc tttttttgca ctatcgatga ttccagcagc tgatgattgt gcaccagcta
     8761 tttctttctc gtgacgtgac tttgcgacca taaaccctaa accaagaccg acaattaaac
     8821 cgatgatagc gaggagaatg ttgaatacca taaaatccac ctccatacta tctttttcat
     8881 ttttatcatc tatacagctt ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg
     8941 catatatgtg tacaaaaaac atgcacaact ttattctaat gtttgtaccc cgtagtgtca
     9001 actttaaata aacaaagaaa cggctttcta cttattcatt aaacgatggt ttttaaaata
     9061 aatgacacta gtttcatata ggtaaacctt taaattgaga aacttttcta ttaaattatt
     9121 ctttgtagat gaacagatat ttttatttta tgtacccact cgtataatta aataactcac
     9181 gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct tcagaccagc
     9241 cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt gtcctttttc
     9301 atcttctggg acagcgactt cttcaccaat accataagct gctctaacta gagcagaaac
     9361 ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc gtccttgacc
     9421 aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt ctttttctac
     9481 tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga catctacttc
     9541 agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg tacggttacc
     9601 aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac ggatcgttgc
     9661 gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca tcacgcctac
     9721 tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga tcgaaccaga
     9781 gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg agtcacccat
     9841 ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta ctacgatatc
     9901 tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc ctgtgtcagg
     9961 ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt gcggatctaa
    10021 cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt ctgcaatagc
    10081 gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga tgatacgtcc
    10141 acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac ttggaattgt
    10201 agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac catagctctt
    10261 ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca atacgataat
    10321 cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc ttttttagtg
    10381 aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta aaagctgttt
    10441 tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac gatctctggc
    10501 aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc atactgttcc
    10561 tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta cactgtaatc
    10621 tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt tgctgactgt
    10681 tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg tttggagcga
    10741 gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc ctaatgcggc
    10801 ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt tttctttcag
    10861 tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa aatattcccc
    10921 tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg cctgaacatc
    10981 atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt
    11041 tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg attcaccaat
    11101 cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac gttcctctag
    11161 taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac cgggaagaag
    11221 gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg tacgatttgg
    11281 tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg tcatttttcg
    11341 atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt gtatgagaga
    11401 cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc
    11461 gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact cttctaaacg
    11521 ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat cagctagctg
    11581 ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt ctgttccgac
    11641 agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc tgtattttta
    11701 tattttttta tt
//
LOCUS       DAFCLG010000079        11341 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFCLG010000079 DAFCLG010000000
VERSION     DAFCLG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.079"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(368..1486)
                     /locus_tag="LND56_001933"
     CDS             complement(368..1486)
                     /locus_tag="LND56_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743843.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="LND56_001934"
     CDS             1957..3264
                     /locus_tag="LND56_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK6743844.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="LND56_001935"
     CDS             3383..4582
                     /locus_tag="LND56_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6743845.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="LND56_001936"
     CDS             complement(4609..5877)
                     /locus_tag="LND56_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBK6743846.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="LND56_001937"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="LND56_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="LND56_001938"
     CDS             complement(7094..7597)
                     /locus_tag="LND56_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6743847.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="LND56_001939"
     CDS             complement(7720..8484)
                     /locus_tag="LND56_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBK6743848.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="LND56_001940"
     CDS             8592..8867
                     /locus_tag="LND56_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK6743849.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="LND56_001941"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="LND56_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6743850.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10434..10634)
                     /locus_tag="LND56_001942"
     CDS             complement(10434..10634)
                     /locus_tag="LND56_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743851.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3401 a         2387 c         2002 g         3551 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa ggaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat
    10321 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
    10381 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
    10441 tcaacgtaga caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt
    10501 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc
    10561 actcccttcg cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg
    10621 gagataccac ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta
    10681 tccatccctt tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt
    10741 cccttcttat aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa
    10801 ctggctcctt tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact
    10861 acaaatttct tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag
    10921 gatggtattt aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc
    10981 tttaaagaca ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat
    11041 gaacttatct tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc
    11101 ataccggaca actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa
    11161 atgacaatga aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg
    11221 cgactgctct tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta
    11281 gttcctcatc tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat
    11341 a
//
LOCUS       DAFCLG010000080        11244 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFCLG010000080 DAFCLG010000000
VERSION     DAFCLG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.080"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="LND56_001943"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="LND56_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK6743852.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="LND56_001944"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="LND56_001944"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK6743853.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="LND56_001945"
     CDS             complement(2159..2896)
                     /locus_tag="LND56_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6743854.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="LND56_001946"
     CDS             complement(2987..3514)
                     /locus_tag="LND56_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743855.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="LND56_001947"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="LND56_001947"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK6743856.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="LND56_001948"
     CDS             complement(4391..4876)
                     /locus_tag="LND56_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK6743857.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5029..5226)
                     /locus_tag="LND56_001949"
     CDS             complement(5029..5226)
                     /locus_tag="LND56_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743858.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="LND56_001950"
     CDS             complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="LND56_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBK6743859.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="LND56_001951"
     CDS             complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="LND56_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBK6743860.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9201..9413
                     /locus_tag="LND56_001952"
     CDS             9201..9413
                     /locus_tag="LND56_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBK6743861.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9415..11094
                     /locus_tag="LND56_001953"
     CDS             9415..11094
                     /locus_tag="LND56_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6743862.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3258 a         2429 c         1971 g         3586 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt attgtacatc
     5041 tatattttct tctattactt tttttaaaat taattcgtac acttctattt gtgctgccaa
     5101 gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct tcgtctgaca
     5161 gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat ttaaaaactg
     5221 ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc aagaatagag
     5281 aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct tcctcagcaa
     5341 attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc tcatgcgttc
     5401 ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca cggattgtcg
     5461 ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc attgcttttt
     5521 gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa atcaagattt
     5581 taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct aaagacacat
     5641 tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg ataaaatgat
     5701 ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca agctttccga
     5761 agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag accataccaa
     5821 atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca atccttattg
     5881 ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc ttgccggcac
     5941 cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc aaatcttgga
     6001 ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca tctcctaaaa
     6061 tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca tctaaaatct
     6121 cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat tgtgtcacgt
     6181 tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt tgaccgacaa
     6241 cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata taagtagcat
     6301 aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca cgaaacccaa
     6361 agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt tcgcgtccat
     6421 atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt agctcaccaa
     6481 gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt tttgagatcc
     6541 agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg atataaaaca
     6601 tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata aaagcttgtt
     6661 gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata tttccttgct
     6721 ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg tctcttctca
     6781 aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt aattgcgaaa
     6841 tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata taagaaaaat
     6901 tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt cctgtaacat
     6961 atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact aaaagaaaag
     7021 tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag gtttttttca
     7081 tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg ggcatttttc
     7141 caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata cgatgatctt
     7201 atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag attctgtcgt
     7261 ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg cagaaaagct
     7321 gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg ccaatcgttg
     7381 tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa atccatctgg
     7441 ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca tttcttcttg
     7501 cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa atagcagcgc
     7561 tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat ctctcacgtc
     7621 tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga tcaattggat
     7681 caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc ctggagaggc
     7741 tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag caaatgttac
     7801 attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg gatcttctac
     7861 aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag ctgctcttgg
     7921 atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga atatatactc
     7981 gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca ctgccccgct
     8041 ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt gtgcaacagc
     8101 catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta cagcaaaacg
     8161 tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg aaagattttc
     8221 ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata acggttcaga
     8281 caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta ctacatacaa
     8341 actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca tcattggtgc
     8401 cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag catcgtttgt
     8461 tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg aataactttg
     8521 gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa tcctcgaacc
     8581 aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga tcataaatat
     8641 cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga tcatacgtgc
     8701 taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata tgaaattgaa
     8761 aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt tctcatctcc
     8821 ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc ctattaatag
     8881 aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca ttgatttttt
     8941 ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata acaattaatt
     9001 gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga taagcaaaaa
     9061 cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga aaaaccaggt
     9121 ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca ctgctttacg
     9181 cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca aaagtacgca
     9241 accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat gtagacgctc
     9301 gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt ctagaaggaa
     9361 gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt taatatgaaa
     9421 ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg tgaaatcggg
     9481 aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc cgggatcaaa
     9541 ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag ctacatcata
     9601 caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga ccacatcggc
     9661 ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc tctcgcatta
     9721 gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga gctccatgag
     9781 atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt cagaacgacc
     9841 catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa tatcgtagcg
     9901 actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt gcataaaatg
     9961 gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa tgctgagatt
    10021 ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat cttttcaaaa
    10081 atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt gcagcaagca
    10141 gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag catggaaaat
    10201 gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac atttgtagat
    10261 gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac aggttctcaa
    10321 ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca aatccaaatc
    10381 cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac aacaagtgtc
    10441 aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg aaaaatcaat
    10501 aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct tcgtttgatg
    10561 aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt acatgcttct
    10621 ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa tggcgatgtt
    10681 ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg agatgtgtat
    10741 atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg tcacctgctt
    10801 tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa agaaatcttg
    10861 gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg agaaatgatc
    10921 aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg acctgactgt
    10981 tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt attcgaacgt
    11041 acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga ataaataaga
    11101 ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa acatgagaag
    11161 ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc tcttaaattt
    11221 atttactttt tcatacaaaa aaat
//
LOCUS       DAFCLG010000081        11199 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFCLG010000081 DAFCLG010000000
VERSION     DAFCLG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.081"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="LND56_001954"
     CDS             6..305
                     /locus_tag="LND56_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6743863.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LND56_001955"
     CDS             378..1739
                     /locus_tag="LND56_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK6743864.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LND56_001956"
     CDS             1761..2021
                     /locus_tag="LND56_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743865.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LND56_001957"
     CDS             2035..2787
                     /locus_tag="LND56_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBK6743866.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LND56_001958"
     CDS             complement(2881..3486)
                     /locus_tag="LND56_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6743867.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LND56_001959"
     CDS             complement(3539..4513)
                     /locus_tag="LND56_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBK6743868.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LND56_001960"
     CDS             complement(4891..5610)
                     /locus_tag="LND56_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6743869.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LND56_001961"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LND56_001961"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6743870.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LND56_001962"
     CDS             7161..8558
                     /locus_tag="LND56_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6743871.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LND56_001963"
     CDS             8639..9079
                     /locus_tag="LND56_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6743872.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LND56_001964"
     CDS             9227..9553
                     /locus_tag="LND56_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6743873.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LND56_001965"
     CDS             9554..10303
                     /locus_tag="LND56_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743874.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LND56_001966"
     CDS             complement(10383..10838)
                     /locus_tag="LND56_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6743875.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3553 a         1958 c         2299 g         3389 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt cattccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattctaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttg
//
LOCUS       DAFCLG010000082        10995 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFCLG010000082 DAFCLG010000000
VERSION     DAFCLG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.082"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(7..2016)
                     /gene="metG"
                     /locus_tag="LND56_001967"
     CDS             complement(7..2016)
                     /gene="metG"
                     /locus_tag="LND56_001967"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK6743876.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2284..2562
                     /locus_tag="LND56_001968"
     CDS             2284..2562
                     /locus_tag="LND56_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743877.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2659..3480
                     /locus_tag="LND56_001969"
     CDS             2659..3480
                     /locus_tag="LND56_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6743878.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3765..4775)
                     /locus_tag="LND56_001970"
     CDS             complement(3765..4775)
                     /locus_tag="LND56_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6743879.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4787..5740)
                     /locus_tag="LND56_001971"
     CDS             complement(4787..5740)
                     /locus_tag="LND56_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6743880.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5730..6800)
                     /locus_tag="LND56_001972"
     CDS             complement(5730..6800)
                     /locus_tag="LND56_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743881.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6806..7849)
                     /locus_tag="LND56_001973"
     CDS             complement(6806..7849)
                     /locus_tag="LND56_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743882.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7850..8791)
                     /locus_tag="LND56_001974"
     CDS             complement(7850..8791)
                     /locus_tag="LND56_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743883.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8875..9222
                     /locus_tag="LND56_001975"
     CDS             8875..9222
                     /locus_tag="LND56_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBK6743884.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9292..10953)
                     /locus_tag="LND56_001976"
     CDS             complement(9292..10953)
                     /locus_tag="LND56_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6743885.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3081 a         2254 c         2013 g         3647 t
ORIGIN      
        1 gaatttctat gcgatttctg agccatttgg catggatttt ggtgcttcga ttacttgtaa
       61 agagccatct ggtgcttctg cagaaaggat cattccttga ctgatttgtc cacgcatctt
      121 tcgtggtttc agatttgcta caatgacgac ttttttcccg atcaattcgc ttggatcagg
      181 ataaaattca gcgattcctg acaagatttg acggtcttgg ctgtctccag catctagtcg
      241 gaattgcagc aacttatctg ccccttctac tttttgacag ttgatgactt ctgctacttt
      301 tagttctact ttttcaaata catcaaattt gatttgtttt tctttcgttg aaacaagttc
      361 tgtttcttct ggatcccatt tgactgtatc ttcgtttgtt tgagtacctt cagacatttt
      421 ttcttgaata aaagcaactt cttcttccat atctaaacgt gggaagattg gtgtaccttt
      481 tgcaactact tttgttcctg aaggaaattc gccaaagtgt aatccttcaa gattcattgt
      541 ttcagagtcc aatcctagtt gattgaagat cttctctggc gtttctgtca tgattggctg
      601 aagtaatatc gctgcgatac gcaaagattc agctaaatgg atcatgacac tattcaattc
      661 agctttcttt tcttcgtctt ttgctaatac ccatggttct gtttcatcaa tgtatttgtt
      721 tgcacgagaa atcagcaccc agatttcagc caatgctgta ttgaattcca ttttttccat
      781 tgcatcatgg taacgtccga caacgttagc agccgtcgta gacagttcac tatcaaatgg
      841 agtgacaagt gaggcatagt caggaacgag tccatcacaa tatttgttga tcatagcaat
      901 cgttcgattc aataagttcc ctagatcatt tgccagatca taattgacac gggaaacaaa
      961 atcttctggc gtaaagacac catctgaccc aaacggaaca gcacgaagca aataataacg
     1021 gagtgcatct aaaccataac gttccacaag catttcagga taaacgacat ttccttttga
     1081 tttagacatt tttccgtctt tcatcaatag ccagccatgc ccaaatactt ttttaggtag
     1141 aggcaaatca agggccatca gcatgattgg ccaatagatc gtatgaaagc gaacgatttc
     1201 tttaccgacc atctgaacat ctgcaggcca gtatttttcg aatagacttt catcttcgga
     1261 gccatatcct agtgcagtaa tataattgga taatgcatcg atccatacat acaccacatg
     1321 tttcggatca ctcttgactg gtactcccca agtaaaagtt gttcgtgaaa ctgcaagatc
     1381 ctctaatcct ggtttgataa agttgttgat catttcgttt ttacgagatt ctggctggat
     1441 gaattcggga tgttcttcat agtattccaa tagacggtct gcgtatttgc tcatgcggaa
     1501 gaagtatgat tcttctttta ccaattctac ttcgtgtcca cttggcgctt taccaccgat
     1561 cacgttacct tcgtcatctt tgtacacttc tgcaagttgt gtttctgtaa agaattcttc
     1621 gtcagaaaca gagtaccagc cttcatattc acctaagtaa atatctccct gttctaataa
     1681 acgatcaaat attttttgaa cagctgcttt atggtaatca tctgtcgtgc gaatgaattt
     1741 atcataactg atatcaagcg ttttccataa tttcttgaca tcagccgcca ttttatctac
     1801 atattcttgc ggttttacac ctagttcttc tgcttttgtt tcgatttttt gaccatgttc
     1861 atccactcca gtgagataaa acacatcaaa gcccatcaac cgtttataac gagcaattgc
     1921 gtcacatgca atcgtagtat acgagttgcc gatatgaagt ttgccgcttg gataatagat
     1981 tggcgtagtg atataaaaag tttccttttc tgccacgggg ctcttcctcc tttatcgcat
     2041 gatgcgaatt cgtttccttg ctagtatacc ataaaaaaag catgctgtta gctttttttc
     2101 aaaatgaaaa agactggata aacgacttct atctacgttc aatctatcct ttaaaaggat
     2161 gaataggttg aataaatgcg ggtttttgtt ttgtttcata tctactaaaa aacggctttt
     2221 tatttctccc ctcacacgtg gtataataaa gactatctat cttagtaagg gactggaaca
     2281 atcatgcaga agaaacatct tttttttaca cttagtattg cttttctttc attagcacat
     2341 ttaatattca gctattttta tattcgtatg tatggttatt ttaacctcca tgggcatttg
     2401 aacagtttta tgacagcagc ttggattttg cgttttatca ttgacgtata tatcgttatt
     2461 tgtggcttct ttgccattcg tgaagagcgg tataaagtat tgccgtttta tctgcttttc
     2521 tttttattca atctcatact tccatttatt tttcatattt aaatgataac ctagattttt
     2581 caagtaaatc cttggttttt tttcagagtc tgctacaata aggtaaaaag tacttttact
     2641 gtcaatggag gtggtcagat gaaccgtttt gcttctaaag aagaagaaaa attatattat
     2701 gaacataaag caagtgaaga agaatttctt aactggtatc aaaaacagga acgtccagaa
     2761 tatgacaagc cctctttgac tgtcgatatc gtactgatgt gctataacaa agaagaagat
     2821 cagctgaaag ttttgttgat ccaaagaaaa gggcatcctt atcgaaattc atgggcattg
     2881 cctggtggct ttgtccaaaa ggatgagtca actggagaaa gcgtcttaag agaaacaaag
     2941 gaagaaactg gtgtggttat ttcaaaagaa aatattgagc aactgcatac attcagtacg
     3001 cctaatcgag atccgcgagg ctgggtcgtc acagtcagct acttagcctt tattggagaa
     3061 gaaccgctaa tcgccggaga tgatgccaag gaagtacgct ggttcacact agaacgtcat
     3121 agtaacaaga tccatttatc tagcggagaa gtggctatct cgcttgattt agtcactgga
     3181 gagtcttctg gcaaagatac tttggctttt gaccatagtc aaatcatctt gaaagcattc
     3241 aaccgtgtag tcaataaaat ggaacacgag ccgcaagttc tacaagtttt aggtaaagat
     3301 ttcacgatta cggaagctcg aaaagtattt gctaaattct tagggatcga ttataaaacg
     3361 atcgatcatt ccaattttaa gaaagctatg ctccaatatt tcgatgagat cggtgaacgt
     3421 cctgtaggaa tcggccgtcc ttcaaaaatc tatcagctca agccggggca ccatgaataa
     3481 ggttgtgagc aaaagattca gacctaatca ttcaagaaaa aacgcaaaaa caactctttg
     3541 tgagttacgt tttttgtttc agaattttga tgtattcgta aactttctga ataaaaaatt
     3601 aacaatagca gaaaaagctc gtacgaactt tttctgctat tgttttattg gtttgtcata
     3661 attagtgagc ttttcttcaa aagaaaaatt cctagaaaca aaagtcatta agacttttta
     3721 ttaacaaggc taaccctaac gctcctatca gtttcattca ttttttatcc cttgataacc
     3781 gaatgtctgc ccattttttc tactacttgg ctctttctta cggaattgct ttcttctgct
     3841 ccgtcggtat tgatcaaaag aaatttttta ttataccatt gggacttata acgcaatttt
     3901 gttattaatt tatgccagtc gtgaatagaa aaatcagcat gtgcctcttc ttttggaaaa
     3961 gattctacaa ataagtagtc atattgtttg accaatttcc cagataattg ttcaagataa
     4021 tcttctcgct ggttcttttg tttcagataa agatccatta cttttctctt ttgtttctga
     4081 taattgcttg cctgatctaa cctgatcttt ctatggtgag cggagcggac tttcacattc
     4141 aatttccgct gtgcatgttg cagcttttct tctgtctggt gaaattttgg taaagttact
     4201 tctagaggtc gagatgtttg gatcaatgtc ttcggatgat aggcgatccc gatccattta
     4261 gctaaagatt ctttttttac tggcgaaaca tcacaaagta gtgcgacata aaattcttca
     4321 ttatatcgag cagaaatcgt agcggagcga atcgatcctt caactggccg atgctgattt
     4381 atcttgatta gttctttttg ttttggtaac tttaagtagc cattttccag atagatcgta
     4441 cgggtttggt tgttggtcgt ataagattgc cacatacttt ttttcgtttt aagctttggg
     4501 aagctggcac gtcccttgaa ataattgcga aaagcccgat ccaaattaag ctgtgcattt
     4561 gctaaagcaa gactgtctac ttcttttaag aatgggtatt ctttttttaa tgtagcgggt
     4621 gtgagcgaaa aattggctac tcctgtttgt tgccaacttt tctgccgctt tttcagcaaa
     4681 tggttatagg taaatcgagc acatccaaaa gtacgaatca attgctgttt ttgttcttct
     4741 gtcggataca tacggaattt aaaagctttt agcatctatg tccctcctag ttagcaacca
     4801 gaagagattc cttttgtcgt tttagttttt ttgcgtaata tgggacttcc tgctcagttg
     4861 catagacaaa atgttctggt gcgatttcac gaagttgacg tacttgtccg tcatctggtt
     4921 caggttgata tttgattctt ctgcgttttc tctcatggtc tggatctggt agaggaatag
     4981 cagataacaa actttctgta taaggatgaa caccgtgatt atagatttca tcacttgttc
     5041 ccatctctaa taacaagccg ttatgcatta caccgattcg gtcactaata tgtttaacca
     5101 tcgacaaatc atgtgcaata aatagatagg tcaattgatg ttctttttgg agatcttgca
     5161 acaaattgac cacttgggct tggatcgaca catctagtgc agaaattggt tcatcacaaa
     5221 tgataaaacg cggttttact gccaatgctc gtgcaatacc aatacgctgg cgttgcccac
     5281 ctgaaaattc atgaggataa cgagtaccgt ggcttggatt caatccaaca gttctcaata
     5341 gatcatcgac tttttttgca cgctcttcag atgatttagc tagtccatta acatcaattc
     5401 cttcggcaat gatatctctg actttcatac gaggattcaa cgaagcgtat ggatcttgaa
     5461 agatcatctg aacatcatgc cggaatttcg tcaaaccttt tttatctttg atcttcgtta
     5521 tatcttgacc gtcgaataat atctcaccat cagtaggttc attcaagcga atgatcgtac
     5581 ggccagtagt ggattttccg cttccagatt ctccaactaa accgaatgtt tctccttcat
     5641 aaatgtgaaa gctgatgtca tttacagcat gtacttcatt cttttttcct acattgaaat
     5701 actgtttcaa tcctttaact tcaagtagtt tatttggcat cagcaattcc tccttgaccg
     5761 taagtatgtt tttgtttctc tttaaagata gcccagcgac gttggatttc ttctggcggt
     5821 gtcactttgg gtgcctgcgg agcaagtagc caagttgctg ccttgtgtgt ttttgatagt
     5881 tcaaaaaatg gtggttcttg ttctgcatcg attttcatcg cgaattcatt tcttggatag
     5941 aatgcgtctc cttttggcgg gttcaataaa tccggtggag aaccaggaat agcatacaat
     6001 cgatcgttcg cactatctaa tgtcggcatt gaaccaagca gtccccaagt atagggatgt
     6061 tgcgggttgt agaaaatttc ttccgatgtt ccaacttcaa cgattcttcc tccatacatg
     6121 acagcgacac gatcagcgac attcgctact acaccaaggt catgagtgat aaagataatt
     6181 gatgtatcga tttttgtctg aatttctttc agcagctcca aaatctgtgc ttgaatagtc
     6241 acatcaagcg ctgttgtagg ttcatcagca atcaggattt ccggataaca taccagtgca
     6301 atcgcgatca cgatccgttg acgctgacct cctgagaact gatgcggata actgttgata
     6361 cgtttctcag cttcgggtat tccaactaat ttaagcaatt ctaatgcagc tttttgtccc
     6421 tctttttttg aaactttatt atgctttcta agggactcag cgacttgctt cccaatgggc
     6481 atcgtcggat ctaaagaggt cattggatct tggaaaatca tcgctatttc tttaccacgg
     6541 attttttgca tctctctttc tgattttcca acgatatcgt tacctttaaa gaggatttga
     6601 ccagcatcta tattggcgtt gcttgacagc aagcgcatga tgcttctggt cgtcactgat
     6661 tttccacttc cagattcccc aacgatcgcc aatgtttctc ctttattcaa atgaaaattg
     6721 acaccgcgaa tcgctcgtac ctttccagca tacgtatcaa aagagatttc taagtctttc
     6781 acttctaaaa ttttcgtcat cgtcttcact ctttcatttt aggatcaaag gcatcacgca
     6841 atccatcagc aagtaagtta aagccaatca tgataaccga taaagttact gccggaatcc
     6901 ataatagata aggcagatac aggaatgtct tgtacccttc gctcaacatc gttcctaatg
     6961 aagctgacgg aggcgtaaga cctaaaccga taaagcttaa aaaggcttcg aagaaaatag
     7021 cagatggaat actaaacatc atttgaataa taatgacact agagatattc ggcaaaatat
     7081 gtttgaatgc aattttcatt cttgattcac ctaatgtcaa tccagctaaa acatattctt
     7141 gatcttttaa tttcattgtt tgtgcacgaa cgatacgtgc cattggtatc cagtttgtga
     7201 tggccatagc agccacgatt gaaaaaattc ctggttcaaa aacgaccaac atcaaaatca
     7261 tgacaacgag gtttggaatt cctgataaga tttccaaaaa acgttgcatc acgttatcga
     7321 cacgtccgcc taacaaacca gaaaccaacc cgtaagctac accaatcgtt acatctagta
     7381 atgcagcaat aaatgcgatc agcaacgaga ttctcattcc cataaataag cggcttaata
     7441 catcacgtcc caaaccgtct gttcctaaaa agaaatttac gtttgaaggg acgtttgctt
     7501 gagcatattt atcaacaagt tcaccaccaa ccattgcttt tccattcaat ccatcgatat
     7561 tgattcccgg aatacgagga ggcaaattga tataagccac gttttgggca gttggatcat
     7621 gaggagaaac aaagattgtg atcacagaga tcaaaatgat gaccaatagt aaaaccattg
     7681 aaataacggc agctttgttt ttctttaaac ggcgccatga atcttggata aaattcaaag
     7741 aaggtgcagt aatctgttca cgttcttctt cggtattttt ttgtaatgga agaaattcat
     7801 ccgcagggat atctgcaact gtttcataac gctttggtat catttccatt tatcctcggc
     7861 tcccttctga aacacgaatt cttggatcaa ctagaccgta caatagatcc acgatcaaaa
     7921 taacaaaaat cagcataaat gagtaaagga tcgttacagc catgatcgta ggataatcat
     7981 ttgttgtgat tgacttaacg aattgttccc cgataccagg aatagcaaag atattttcta
     8041 ccaccagtga tccggtcatc aatgctacag ctaacggtcc taataaagtc atcaatggaa
     8101 tcaaactatt tcgaagacca tgtttaaagg cgatttccca acggctcaat ccttttgctt
     8161 ttgctaattc cacataatca ctatgcagta cttccaccat ttccgtacgg ataaaccgag
     8221 cagagtcagc aagcggcgac atggccaacg caattgttgg taagattgtg tacacaaagc
     8281 catcccattt tgcgattggc aaaacttgta atttgattgc aaagatatat tgtaataaaa
     8341 cagcaaatac aaagtttgga atggaacgac ctaatatcgc tacgagtgta gaagatgtat
     8401 ctgcccatgt attttgcttc atggcagaga tcgttcctaa aatcgtccct aggactgttc
     8461 caaagataat tgcctgtaac cctaactgca aggatggtcc gattcttcca gctaacaaat
     8521 gtgcgactgg ctggttttta aattggaagg aaattccaaa atctccttga agcaaattag
     8581 aaagataaat cccatattgt acgataactg gtttatccaa accgacttgt ttatttagca
     8641 tctcgattgt ttcaggactc aaacgttcct gattcgtata tggcgtgccc ggcagcaatt
     8701 gcatcaagaa aaaggtgatt gttgcaatta accaaagcgt gatgatcatg aaaaagacac
     8761 gttttaatac atatttgata taactattca ctaatgtttc cccctagctt ttgacttctg
     8821 acagatgttt cgctacatta ttagataatg aaccaaaaat aaaggagcaa tcaaatgaaa
     8881 aaaaaatcta tcccctacgc agtagctttt ctactgattc tggttatttt aataaaaaat
     8941 ctcatcaacc attcctttac actcattcaa ttatctaatg atctattctt atggtcattg
     9001 ccgtttctga ttatcggcgg ttttttatgg gtcttttctt cgggtttctt cgatcatttt
     9061 cagcgatcgg tccatcttgc acgaacaagg aaccgaaaga aaaaaccaga attttcctcg
     9121 ctctcgtctg ccagctacgg tatgtacagc ttttggctga tcattgccgg aatactaatt
     9181 gctctttctg ctattttcat gcttttttct ttgttaggat aagtgaatat gagaacgagg
     9241 ttgcgacatt tgccgcagcc tcgttttttc tcatgctcac ggtattaacg attattcttc
     9301 aacgtatgtc catttgtaat cccatgaagc gcctgccgca tggtgaacga tacctttgat
     9361 tttttcattc actaggtgac cttctgcttt ttggtacacc ggaacgacac ccatatcatc
     9421 agagatgatc ttgattgctt tttggaaatc atcccaacgg gcttgttcat cgccagcgtc
     9481 tgttgatgaa gctgctttaa ctgcttcgtc atattctgca ttgctccagc gaccacggtt
     9541 gtatgaatta cctgtgacaa acaaatcagt aaagctgctt gggtctgcat agtcagcagc
     9601 ccatccgcct agtactgcgt caaaatcacc cgaagttgag cggtcaagac gtacagagaa
     9661 tggaacaggt gttggtttga ctgttacgcc atccaagttt tcttggattg agttttgaac
     9721 atattcgatt acttttttcg atgaatcatc atcagaagcc atcaaatcaa attctaaaga
     9781 atcaacgccc aattcttttt tcgctttttc ccaatattct tttgctttat ctttgtcata
     9841 agaaactaat tttccagctt cttttgcaaa gtcttcgttc gttgttggac tttttaccat
     9901 atctgatggg atcaatcctg tagagacaac tgatccgtca cctagtactt gttttactaa
     9961 agcttcacga tcgatagaat aagaaatagc ttttcttagg ttcacattgt tgaatggtga
    10021 attcttttca cgttgattga attcgatata gcttgtccgc gcttctttaa cagaagtata
    10081 agctggatcg tttgcgtttt gctgtgctaa ctcaccagac aagatcacgt catctgcttg
    10141 gccatctttg aataagttca atgcagtaga agattctttt actacactga ctttgatttc
    10201 agaaagtttt actgtatctt tgtcccagta atgatcattt ttcttataag tccattctgt
    10261 atctgttcct ggtccatcaa aatcttctaa tgtgaatggt ccattataaa cagctttatc
    10321 acttgcagat gcataggcat ccccattttc ttccactacg gattgttttt gtgggaaaaa
    10381 tgatgggaaa gcaagcagat attggaaata tggtgtttgt ttcgtcaatt tgatttctaa
    10441 ttcatagtca ccaactgctt tgatccctag ttctgattta tctttctttc cagctgtaat
    10501 gtcttctgca ttttcaactg gcgcatacag gtaagcatat tcagaagctg tagctgggtc
    10561 tactgttcgt tgccagccat aaacgtaatc cgctgctgta acaggatcac catttgacca
    10621 ttttgcgtct tctcgtaatt tgattttgta agttaatccg tcttcactga cttctgccaa
    10681 ctcgctagct cctgccggct gtggtttgct gtcttcatca agacggtaaa ttccttcata
    10741 aacgttattc aatgctgtaa aactgattgt atctgttgca actgataagt cagctgtagg
    10801 catttcttga ggcacgacta agttaaatac gcctcctcca tctgctgaac cgctttttgc
    10861 tgaactactg cttgtggctg aactgccgcc ataacatcca gcaagtacaa ggccgcatac
    10921 cgcaaccaaa ccaaatgtaa atttcttttt cattttacgt acccctttat tggaattttc
    10981 agaataatta atatt
//
LOCUS       DAFCLG010000083        10858 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFCLG010000083 DAFCLG010000000
VERSION     DAFCLG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.083"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            152..589
                     /locus_tag="LND56_001977"
     CDS             152..589
                     /locus_tag="LND56_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743886.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            684..1568
                     /locus_tag="LND56_001978"
     CDS             684..1568
                     /locus_tag="LND56_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6743887.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1792..4959
                     /locus_tag="LND56_001979"
     CDS             1792..4959
                     /locus_tag="LND56_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743888.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4979..5524
                     /locus_tag="LND56_001980"
     CDS             4979..5524
                     /locus_tag="LND56_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBK6743889.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5552..6007
                     /locus_tag="LND56_001981"
     CDS             5552..6007
                     /locus_tag="LND56_001981"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBK6743890.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            6004..6762
                     /locus_tag="LND56_001982"
     CDS             6004..6762
                     /locus_tag="LND56_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6743891.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6848..7588)
                     /locus_tag="LND56_001983"
     CDS             complement(6848..7588)
                     /locus_tag="LND56_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6743892.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7649..8902
                     /locus_tag="LND56_001984"
     CDS             7649..8902
                     /locus_tag="LND56_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6743893.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9224..9451
                     /locus_tag="LND56_001985"
     CDS             9224..9451
                     /locus_tag="LND56_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6743894.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9574..9900
                     /locus_tag="LND56_001986"
     CDS             9574..9900
                     /locus_tag="LND56_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6743895.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9968..10681
                     /locus_tag="LND56_001987"
     CDS             9968..10681
                     /locus_tag="LND56_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK6743896.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         3699 a         1823 c         2259 g         3077 t
ORIGIN      
        1 gaactaaatc aacagtagtt taattactac ttgatttagt tctttttatt ttaaaaattt
       61 tttttactta gaatccagcg accctagata tttttctgta taatcttaaa taaagaaaac
      121 aatatcaaaa aatgcacgta gaagtagata aatggagtgg ttttgcaaat ttcttttatt
      181 tctagaagtt attcacgtat acggatttgt ttttctcaca aataaaaagg caggaagtac
      241 aatggatcat ttgtatataa aatatattca tcaattttcc gttttggtgt taggcacact
      301 aattatagcg gggattgtcg catttattat tcaaggccgt gacatattaa cagtacatca
      361 agagtttttc agttatgcat ttggtattcc tctgatcgct ataaggtctg ataacaaaaa
      421 gtggaagaaa cgctattaca ttctcttgct atttttgtgt ttttatctgc cacctaagat
      481 ttttacttta ttaggtatac aatgcctatc attttctttt atttcctata tcattgtttt
      541 aattttcctc agtttcatag tatggatgtt ttatcttgtc aaaaaataaa tgaatgtttc
      601 atgaacagta cttcatcaca gcctaatttt tttctttatg ctatcgttat attaaagaaa
      661 tcaatcagaa gggaaagaaa gtgatgaaaa aagttttatt agtggtaaac cccagttcag
      721 gcggtgaaca agcaaaggaa tttgagcaat tagcgatagc aaaattggaa tcggtgtttg
      781 atgaagtggt cgtcttgcat acaaaaaaag caggggatgc aaaaaacttt actcgcgaag
      841 cggctacgga agggtatcat agtgtgtttg tcatgggcgg agatggaaca gtcaacgaag
      901 gaatcagtgg aatcgctgaa caagaacatc ggccaaattt cggatttttc ccattgggta
      961 cggtgaatga cttggctcga gcattaggga taccgctaga acctgaggaa gcgatcaatc
     1021 atttttcgat tgaatcagtc aagccattgg atattggaaa aataaatgac gactatttta
     1081 tgaatgtcgt cgcaattggc tctatccctg aatcaatcaa tgatgtagat gcggagaaga
     1141 agacgaaatt tggaaaacta gcttatttca tgtcaggaat caaacagtta gcttccacac
     1201 aatcctactc gtttcacgta gaagttgatg gaaagaagga agaaattgag agcagtacct
     1261 tgctgatagg actaactaac tcagtcggcg gttttgaaac attacttcca aatgcaaaag
     1321 tggatgatgg taagctccat ttggtttatt taaaagattc ttctttgatc gatacgctta
     1381 aaacattgcc agacctatta aaaggagtag atgaatctac gaaaaatcta gcctacctta
     1441 cttgtgagca attagttgtg tcattagctg atgaggaaga attgacgacg aatgttgatg
     1501 gagatgaagg ggataaatta cctgtgacaa tcaaagtatt gccctcacat ttacaggttt
     1561 attgttaaaa tggaggttgt gaccgaggag ttggcgggag aacaccgctt gttcgggata
     1621 agggactgtg acaagacttt tgtcacagcc ttttttttgt acaaaaaaga aatttacacc
     1681 taatagaaaa atttttccat aaaaatacat attttaaata tgaaagtaca attggaaata
     1741 gattaaaacg ataaaataaa attgtcaaaa cgtcagagag gatggtggag aatgaatcaa
     1801 tgtgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat ggtgacatcc
     1861 gtttttgccg atgtagagaa aacaaacgaa tcaacgcaat cagagaatca aagttttgac
     1921 aagactactt tttcagaaga atcgattact tctgaaacta cagaatctca aagtgaggct
     1981 tctactaata aaaggcaaga agaaaataag acaagtgaga cctcagaagt agctgaacaa
     2041 acgtctgata gcagtgaagc ggcactggca agagctgttt cagcggatgg gtacagcgaa
     2101 gctgccacaa cagaagaact ggcgcagctt ctcgcagacg aatcagtaac taagatccga
     2161 ctgattcagc ctttgacttt agacagggaa ttagagatca aacgagatat cgttattgat
     2221 tttggtggat tcgctcacaa tttcggcaca caccatattt atatcaatga gatacctcag
     2281 tcgatcgagt tccagaattt taaaggaaca gctgcacatc caggtggatt gacaccaaat
     2341 atcgatggaa atgccattat cattgcttat atgaccgatt tttggggaag cagatatcat
     2401 tttacgggag aaataaaatt taccgggaca cttgatttgt atgatgggtc caaattagga
     2461 ctgatttacg ctcctcgtgc aactgtcaca ttagatggtg tttccggtgt gttggatgtc
     2521 caaccagtga aggaaggact gaatgccgct ccgggtaaag cctatttttg tagaagctat
     2581 caattgaatg tgatcaatgg tagtcaactt tatggaccgt atctagggaa attctacggt
     2641 tttctagatt caggagatga gactggtagt ggaaaaacag cgccaggaat ccatatctta
     2701 agcggttcaa aggtctctct tgattatgat cgggcagatg gcgttagcga tgaaggagag
     2761 gcagtcgata cgctacctgg taatgtcatg tttgaagtca gtggttcggg aagcgaattt
     2821 tcggtaaata caaagatcaa tattaccaat gacaacaatc gcggaatcat ccaaatgaga
     2881 ggttcaggat cgaaagtcac tgtatcgaat ggtgggaaaa tcaccatcaa tacagcaact
     2941 acgggcggat ttcgcttgca aggagattct agtcaaatcc atgtatcaag tggtggacaa
     3001 atctcggtca aaatggctgg agatgacgat caacccggga acaatgggat gcgttttgta
     3061 ggaaaaggcc tttctttgat cgtagaagga gccggatcag ttatcgacat cgataagcag
     3121 agtgggcgtt ccagcgcagt tcgttttgag aatggtacgc aaaagctgac agtaaccaat
     3181 ggaggctcat tgagggtaag aaatacaggt gatggcaaat cttacgtgaa cagactcaat
     3241 caaggaaatc aagcgatcca attttatgac gcttctggtt ttctagaaag tccaggttca
     3301 gctgatttta cagtcactgg agaaaaatca aatatcgata tacgcgcaga ttccggagca
     3361 actgtagata caactggaga tattgattta aattttacgg caggtgaaaa aacttattta
     3421 gtcatggtcg ggaaaacagg ggaagataag accggcatca tcagtgggga tgtgttgaat
     3481 gtggacttag aatctcctac ctattttgat ttccaaaaca aacggattgg aagcgagacc
     3541 aatggcatag gcggctgggt attccaagga aatggagatt cttctttaaa gatcgcccat
     3601 tcagaaatcg ctttatggcg aaaaacaggc gcaacattcg ataactttga aaaagacccg
     3661 gattattttt ctgatatgac agacttatcg atcaatggga ccgacttagg caatttcgta
     3721 tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg aatgaaaaat
     3781 tataatcgaa tcagtgccaa taatcagctc ccgatgattg aatccttaag agtgccaacg
     3841 aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga ggcaaagcca
     3901 agagatgctt ggacaggcga agtagaagtg actttacgta ttacttacgc agatacgacg
     3961 aaatcatctg tagaaattac tgctctcacg caaggacaaa cagatgaaaa tgaaggggca
     4021 ggatataacc catggggaga aggcgaacaa ggcggcttgt tcgaagttca agtacccaat
     4081 gatgagagat taaaaacgga ggatcaagtg caagttgttg ctgctaaaaa agtaagaggt
     4141 ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat tcctcctgaa
     4201 cctgctaatt taagtaccac attgatcaat gcagcttctc gcacgatcac aggtacaggt
     4261 gaagcaggag ccttggtttc attgaaaaaa aatcaagctg ttttgtcaga aacaactatt
     4321 gatgatacag gggaatttgt tctttctatc ccagaaaatc aactttttgc aggagatgta
     4381 ttagaaattg tattaaacga ccaagcagga gaagctactg ctaagggtgt gcagaacaag
     4441 ccaagtacca acgatgaaat tgggaatcac aatccaagtg atacgccagt tgtttatcat
     4501 gatgccccgc cttttgcacc tgctacgaag atcgtagtag aaggtggtct ctcatttctt
     4561 gccccttcca aactcgattt tggtcagatc aaagcaactg gtttgacaca agaagcattt
     4621 ggccaaatgg atatacagga aaaattatgg atttatgatc agcgagaaac gaaaaatccg
     4681 tggatcttaa cgataaaatt atctcagcct ttcacaaatg atgcaggatt ttctatgcag
     4741 gatgtcctat atttcaagaa aggagaggag tatcaattag tgaatgaaga agaactggaa
     4801 attgtcagag gacaaaattc aacgaatgtg caagcagatt attcagaata tttaaataac
     4861 ggtcgggcgt ttaaacttgt cttaaccaaa gaaaatcaga tagctggtaa ttactcagca
     4921 gagataacat ggacattagc agatgcgccg acagaataaa gatcaaggag gatgattgat
     4981 gaatcgaata tggaataaaa gtcatcgaaa caactgctct ttacttatga tccttctcct
     5041 tggattcatc ctttggcaga cacctgtcca tgcctccgaa ttcaattttg cggtagattt
     5101 tgaattaccg gctaatcaga ttgataagaa caaaacatat ataaatgtaa ggatggagcc
     5161 tgggaaaaca caggaaatcc cctacacggt ctttaaccga acagataaat cagtaacagt
     5221 tgagacattg atccactccg ctacgacgaa ttcaaacggt gtgatcgaat atggagaatc
     5281 aaaaagtaaa tttgatgaaa cattaagtta tgatatgaaa aacttagcaa aaacagtggg
     5341 agaaataact gttcctcctc aaagtgcggt cactgagaca ctttcactga aagcgccaga
     5401 aaaagagctg gctggtgtgg tcgcaggagg gatcacgttt caagaaaaaa aagagaacgt
     5461 ttctaacaaa agtacagttg gtgtcgtcaa tcagttttct caagtgaaag caatcttgat
     5521 ttgaaatagc gaaaaagagt cttccccaga tttgaaatta aaaaaagctt atgcggatca
     5581 aagcgatgga agaaatgtga ttaaggcgaa tcttcaaaat ccaaatgctt cttatatgtt
     5641 cggcttgact acatcggttg aaatctacaa aaaagaaaca aaagagcctt acctttcaat
     5701 aaaaaaagaa aaaatagatg tagcacccaa tacaaatttt gatttacttg tcccattaga
     5761 aggaaaaaaa ttagctccgg gacattatca agcggttgtg aagggtgctt ggaaagaaaa
     5821 acgctggaat ctccataccg attttgaaat aactgatgat gcagcggacc aattgaatga
     5881 aaaggatatt gatttaaaaa atcagccaga aacatttgac tggcgatggc tgatacttgt
     5941 tggagtcatt cttattggct tatttgctgg attatttatt ttaaaagaaa gaaggaaaaa
     6001 gaaatgaaaa aaacaattgc attaagtacc gttctagctg gaagtctatt attatgtaca
     6061 caaagtgttt ttgctgagaa taagagctca gccactacag aaggaacgat ttctgtgacc
     6121 aaaccagatg ctgcaaatcc tgaagacgtt gttccagttc ctcctgttga tccaaatgat
     6181 cctgatactc cttttacacc tgacgaacca aatcagggaa catccggatt actgacgatt
     6241 gaccaagctc cagatcttga ttttggaaca atcaagttag gtacagcaaa aacagtatat
     6301 gctgcattac aaagcggaac ggacagtact ggagcacaaa cacaagtaca gaatttcgta
     6361 caagtaacgg ataaaagcgg aaattatgcc ggttggaaat tatcagtcaa acgaacagaa
     6421 tttattggta caacatctga tacgaataaa ctaactggtt caaaggtgaa attcaaaaac
     6481 gccgtgattc gaaatagcgc aactaatagc agtggaatgc catctaccgt ttctggaact
     6541 ggcgctgaag gaatcgaaat cccagcagat caggcagtag atctagtaac tgcaggagca
     6601 aatgaaggga ttggcacatg gatttattca ttaggagaga atgtggaaca agggagacaa
     6661 tcagtggaat tatttgttcc tgtatccaat tatgctgtag acaactacgt ttcaacattc
     6721 acatggtcgt taacagatgc tcctacagaa gaggaagcgt aaaatcatat caacgatttc
     6781 agggagtgga gccgattcaa ggcccactcc ctgaaatctg ctatgcttac gtctcagctc
     6841 ttattcatca actcgaagca gttttttgga agtcatggaa acgttccttt gtcttttcaa
     6901 agatctttcg accaacgagt aaggagtcac cgttgaaaaa ttctaacaca taatgagcag
     6961 aatcaatttt ctgcaattgc gataagttga tacagtaaga ttttaaaagt aaaaagtaag
     7021 catacttttc taactctgtc ttcaattgtt ttaaagaagt attcactaat ttatcttctg
     7081 tagttgtttt gaccataact tggtttttca aacctttgat actttcaata tataaaattt
     7141 cccgaggata caggacgaga cttccacctt tttgatcaat aaagatttta tcgacattta
     7201 cgatctgctg ttctttccaa ttgatctgtt ctagcacttg acgaatttgt tcagataaat
     7261 tcctgtcttt tctgatatat ccaatcggaa ggattttccg attgatgatc tctactgcaa
     7321 aattcgtata agcggtgaaa aaaacaatat tactatgtgc atttttttta cgtatttgtt
     7381 cggcgacatc aatacctgtg agtgattgct tcaagtcgat atcgataaag aaaacatcgt
     7441 gattatcaat agtgcccaac tggatcaact gattgatttg agagatagaa gtgagctgca
     7501 cgatattctc gatatgatga ttgaccaatt ctctgatgat ccattcgctg tgaaactggt
     7561 tatcttctat gataaaatac tttttcatat gcaggctcct attaaaaaaa tatgtgtaca
     7621 ttttgaaaca aaacaatgga ggaataagat ggtcgcaaca gtgacacttt atttgacctt
     7681 tactttgatt ttagatttag gtatctattt attcttttcg aaacaactgc tagtaaaaaa
     7741 cagctttggt tgtatagctt tgatggtggt gacactttta cttcctatcc ctaaagtgag
     7801 ctttccttac ctagttggct tattattgat gtttcttttt tattttacta cgaaaaacct
     7861 gtttatcagc tgtttgatca gtaatctttt ttttgctatc actggactca gcttttttct
     7921 ggtttttgat ctgatggatt atttaggaaa gagacatctt ggaacagcaa gtttttttgc
     7981 actacttatc gcatttattt tctatctatt agtaaaaagt atagacaaac agacgagatt
     8041 tctcaacttg ctttttactt atccaactga acactatttt ggaggtattt gtattttatt
     8101 aagctggggc gtgatttact tggtattggg aagtgttcac ttccgctcat ctaattatct
     8161 gcttctatct cttttgtcga tagtcatcag ttttttttct ttgttttttt ccattatgtt
     8221 gatcagtttc caagtaaaag aaagacaaca tcaggaatcg cttcaactgt atcaaataaa
     8281 tgaagaatat taccatcatc tagaggaatt caaacatgat tataaaagtc tactgttttc
     8341 attaaaaatt actcttcagg aaaataaaaa agaaacgttg gaatttttag aaatattaga
     8401 agatcattct gcagctattt ttgaaaaacc gcaaatacaa cagctaacaa atattgtatc
     8461 cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa gcagaatcgg agaaaatccc
     8521 gatccaagta gttattcctg tcgaagtgaa aacaatcccg attgatctgg ttgtttttat
     8581 tcgctgttta tctattttta tttctaacgc attcgatcat cgtgtagccg atcagagtcc
     8641 gattacgatc aatctgctgg aagaaaaaga gggatcacgt tttagtatag cgaacaaggc
     8701 aaatccaacg aatttgacgc tatcggagat ggtcaaaaga ggaaaaacct ctaaaaaaaa
     8761 tggcgggtta ggtctataca gtgcgaaaaa aatgttggat gtttacgaaa atgcagaact
     8821 aaaaatcgaa ttttcaaaac agaatcacta tttttttgta gaaatgtatc tggcatctag
     8881 aaagagcaaa tcagcctact gacttactgt caaaggataa gtttgaggaa agtctacacg
     8941 gagatagaga caacaacaca gcatattcaa aagaaacaga agcgtgatga ccgttgtcac
     9001 ccttctgttt cttttgctat gtagttaaac aaagtagttg acacttccgc tacttagtat
     9061 tagtacgaaa gtatacttca tgatactaag tagataagaa gtgaaaagcg gaacccacga
     9121 tcgtcaaaat gctaactgct ttgcttacac cagataaagg gaagagcaat atcgcttatt
     9181 ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa aaaatgctga cccagcagtt
     9241 aagagagtta gaacaagatg aaagtatcca tcgaaaagcc tataaccaag tcccgcctaa
     9301 agtagagtac tcgctcaccg aagaaggaaa atcattaaga gagattctag tagcgttgtc
     9361 tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca agggaagaag acacgctact
     9421 gcatacacat ggtggctacc taaattattg attttctatg acatttattt taaataaaag
     9481 aaaacaggag tttttttaaa agcatcatct attttttatg taaaaaaatg gtatgattaa
     9541 tcaagtaaga agtgattgga agaaggcaag caaatgaata gtatggaaaa agtcaaccag
     9601 tttagagatg aacgtaattg gcgacaattt cataacgaaa aagatttggc tatctctatt
     9661 tctttggaag catctgaatt gttagaactt tttcaatgga aaacgccgga agaagtaaaa
     9721 gaaacacaat tagaacgaat caaagaagaa ctggcagatg tattgatcta ttcatacatg
     9781 atggcagaca accttcacct ggatctagat gagatcattg aagaaaaatt gatcaaaaat
     9841 aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa aaaaatacac agagttgtag
     9901 aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac agtaaaatga gtctggaggg
     9961 agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta gtcaatatcc tcttgttcct
    10021 cttgttatta gtaggactag ctttgatttt caatgaacag atcaaggatt attttgtcag
    10081 agagacagga gataaatatg ctatagcgaa tgtaacaaaa gaagatctga aaaagaataa
    10141 cgataaagat gtcagttttg actttgatgc ggtagaacca atgacgacag aaggggtcat
    10201 gaggtcgcaa atgagaggaa cagatctacc agtgattgct agtatcgcag tcccttcagt
    10261 ttcaatcaat ttaccggttt tcaaaggctt ggataatacg tcactacttt acggagcagg
    10321 aacattatct ccagatcaag aaatgggaaa aggaaattat gccttagcaa gccatcgtgc
    10381 aacgaaccct gaattgctgt ttacaccgct agaaaattta gagatgggtg ccaagatcta
    10441 tttgacagat ttagaaaatg tgtatacata caaaacattt tttaaagaaa aagttgctcc
    10501 tacagatacc caattactga atgaagtcga gggaaaagaa attgtcacac taattacttg
    10561 tggcgacatg gacgccgtca cgcgactagt tgtacaagga gaactggaaa gtgttacatc
    10621 gataaaagat gcgacagatg atatgagatc tgctttcaat ctagagacaa aaacatttta
    10681 ggatactggg acataagtcg ttacagatta aaaacagcca aaggatatga atcgataagg
    10741 aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt gcatctctga tttggttgtt
    10801 ttctagatag acttgtgttc cagttttttt attttttata tgaaaaggct tgcataaa
//
LOCUS       DAFCLG010000084        10665 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFCLG010000084 DAFCLG010000000
VERSION     DAFCLG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.084"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(183..1031)
                     /locus_tag="LND56_001988"
     CDS             complement(183..1031)
                     /locus_tag="LND56_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6743897.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1030..2097
                     /gene="gnd"
                     /locus_tag="LND56_001989"
     CDS             1030..2097
                     /gene="gnd"
                     /locus_tag="LND56_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBK6743898.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="LND56_001990"
     CDS             2099..3100
                     /locus_tag="LND56_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBK6743899.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="LND56_001991"
     CDS             3097..3381
                     /locus_tag="LND56_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK6743900.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="LND56_001992"
     CDS             3414..4211
                     /locus_tag="LND56_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6743901.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="LND56_001993"
     CDS             4225..5058
                     /locus_tag="LND56_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6743902.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="LND56_001994"
     CDS             5076..5933
                     /locus_tag="LND56_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6743903.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="LND56_001995"
     CDS             5955..6464
                     /locus_tag="LND56_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6743904.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="LND56_001996"
     CDS             6501..6902
                     /locus_tag="LND56_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743905.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="LND56_001997"
     CDS             7014..7367
                     /locus_tag="LND56_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743906.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="LND56_001998"
     CDS             7401..7844
                     /locus_tag="LND56_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6743907.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="LND56_001999"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="LND56_001999"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6743908.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="LND56_002000"
     CDS             8939..9652
                     /locus_tag="LND56_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6743909.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9859..10008)
                     /locus_tag="LND56_002001"
     CDS             complement(9859..10008)
                     /locus_tag="LND56_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743910.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10404..10604
                     /locus_tag="LND56_002002"
     CDS             10404..10604
                     /locus_tag="LND56_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6743911.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3558 a         1690 c         2315 g         3102 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9901 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
     9961 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10021 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10081 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10141 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10201 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10261 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10321 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10381 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10441 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10501 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10561 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10621 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaatt
//
LOCUS       DAFCLG010000085        10574 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFCLG010000085 DAFCLG010000000
VERSION     DAFCLG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.085"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..84
                     /locus_tag="LND56_002003"
     tRNA            12..84
                     /locus_tag="LND56_002003"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="LND56_002004"
     tRNA            88..160
                     /locus_tag="LND56_002004"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="LND56_002005"
     tRNA            168..249
                     /locus_tag="LND56_002005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="LND56_002006"
     tRNA            267..339
                     /locus_tag="LND56_002006"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="LND56_002007"
     tRNA            351..422
                     /locus_tag="LND56_002007"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="LND56_002008"
     tRNA            439..524
                     /locus_tag="LND56_002008"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="LND56_002009"
     tRNA            535..608
                     /locus_tag="LND56_002009"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="LND56_002010"
     tRNA            618..691
                     /locus_tag="LND56_002010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="LND56_002011"
     tRNA            711..783
                     /locus_tag="LND56_002011"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="LND56_002012"
     tRNA            811..884
                     /locus_tag="LND56_002012"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="LND56_002013"
     tRNA            898..971
                     /locus_tag="LND56_002013"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="LND56_002014"
     tRNA            995..1084
                     /locus_tag="LND56_002014"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="LND56_002015"
     tRNA            1097..1170
                     /locus_tag="LND56_002015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="LND56_002016"
     tRNA            1189..1264
                     /locus_tag="LND56_002016"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="LND56_002017"
     tRNA            1266..1336
                     /locus_tag="LND56_002017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="LND56_002018"
     tRNA            1350..1423
                     /locus_tag="LND56_002018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="LND56_002019"
     tRNA            1448..1535
                     /locus_tag="LND56_002019"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="LND56_002020"
     tRNA            1557..1628
                     /locus_tag="LND56_002020"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="LND56_002021"
     tRNA            1651..1740
                     /locus_tag="LND56_002021"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="LND56_002022"
     tRNA            1753..1826
                     /locus_tag="LND56_002022"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="LND56_002023"
     tRNA            1845..1917
                     /locus_tag="LND56_002023"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="LND56_002024"
     tRNA            1921..1993
                     /locus_tag="LND56_002024"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="LND56_002025"
     tRNA            1998..2068
                     /locus_tag="LND56_002025"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="LND56_002026"
     tRNA            2081..2154
                     /locus_tag="LND56_002026"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="LND56_002027"
     tRNA            2166..2237
                     /locus_tag="LND56_002027"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="LND56_002028"
     CDS             2503..3696
                     /locus_tag="LND56_002028"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK6743912.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="LND56_002029"
     CDS             3826..5298
                     /locus_tag="LND56_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6743913.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="LND56_002030"
     CDS             5381..6169
                     /locus_tag="LND56_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK6743914.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="LND56_002031"
     CDS             complement(6236..6904)
                     /locus_tag="LND56_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBK6743915.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="LND56_002032"
     CDS             7019..8731
                     /locus_tag="LND56_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743916.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="LND56_002033"
     CDS             8734..10506
                     /locus_tag="LND56_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6743917.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DAFCLG010000086        10565 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFCLG010000086 DAFCLG010000000
VERSION     DAFCLG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.086"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(78..2198)
                     /locus_tag="LND56_002034"
     CDS             complement(78..2198)
                     /locus_tag="LND56_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6743918.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="LND56_002035"
     CDS             2428..3606
                     /locus_tag="LND56_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBK6743919.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="LND56_002036"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="LND56_002036"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK6743920.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="LND56_002037"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="LND56_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBK6743921.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="LND56_002038"
     CDS             complement(4959..6212)
                     /locus_tag="LND56_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6743922.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="LND56_002039"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="LND56_002039"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK6743923.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="LND56_002040"
     CDS             7789..9135
                     /locus_tag="LND56_002040"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK6743924.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="LND56_002041"
     CDS             complement(9443..9829)
                     /locus_tag="LND56_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6743925.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="LND56_002042"
     CDS             complement(10097..10246)
                     /locus_tag="LND56_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743926.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3381 a         1906 c         2117 g         3161 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtg
//
LOCUS       DAFCLG010000087        10496 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFCLG010000087 DAFCLG010000000
VERSION     DAFCLG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.087"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..360)
                     /locus_tag="LND56_002043"
     CDS             complement(<1..360)
                     /locus_tag="LND56_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK6743927.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="LND56_002044"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="LND56_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK6743928.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="LND56_002045"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="LND56_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK6743929.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="LND56_002046"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="LND56_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK6743930.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1964..2089)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2240..2872
                     /locus_tag="LND56_002047"
     CDS             2240..2872
                     /locus_tag="LND56_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6743931.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="LND56_002048"
     CDS             complement(2874..3779)
                     /locus_tag="LND56_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6743932.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4523)
                     /locus_tag="LND56_002049"
     CDS             complement(3864..4523)
                     /locus_tag="LND56_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6743933.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="LND56_002050"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="LND56_002050"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBK6743934.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="LND56_002051"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="LND56_002051"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBK6743935.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="LND56_002052"
     CDS             6766..7851
                     /locus_tag="LND56_002052"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBK6743936.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="LND56_002053"
     CDS             7848..8912
                     /locus_tag="LND56_002053"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK6743937.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9161..9964)
                     /locus_tag="LND56_002054"
     CDS             complement(9161..9964)
                     /locus_tag="LND56_002054"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK6743938.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="LND56_002055"
                     /pseudo
     CDS             complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="LND56_002055"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10271..>10496)
                     /locus_tag="LND56_002056"
     CDS             complement(10271..>10496)
                     /locus_tag="LND56_002056"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBK6743939.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3106 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagcaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9001 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaacgctt ctgtcccagc
     9061 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caagccgctg
     9121 atttcacaac ctctccacta aacggtgtac taaagctctt ttaaggttgt tttccgatat
     9181 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     9241 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     9301 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     9361 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     9421 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     9481 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     9541 taccgccgcc tttttcgatc atatcccgaa taactgcttt tgaaacgatg aatggggcat
     9601 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9661 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9721 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9781 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9841 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9901 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9961 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    10021 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    10081 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac gactaaatgt
    10141 tttgtttcat ctggtttgcc catcatatgg aacacacgtg tatcttcatt ggcataatca
    10201 aaatagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagca
    10261 cttcctggtt ctaaaatcgt ataacccatt tataaatgac agctatcgct gaagcttcta
    10321 ctgctgtaaa tatccccgtc aagataccac cgataatgat gacaatcaat agaaggctag
    10381 gaatcgcctc ttttattatt tttccagcat gagccatctg tcctttttcg acaacagggt
    10441 atttattctt tttagcaacg atgaatgcta ctagcatgat tcctagcgcc cacaaa
//
LOCUS       DAFCLG010000088        10476 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFCLG010000088 DAFCLG010000000
VERSION     DAFCLG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.088"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..656
                     /locus_tag="LND56_002057"
     CDS             33..656
                     /locus_tag="LND56_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743940.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="LND56_002058"
     CDS             1106..2155
                     /locus_tag="LND56_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6743941.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="LND56_002059"
     CDS             2152..3033
                     /locus_tag="LND56_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6743942.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="LND56_002060"
     CDS             3036..3806
                     /locus_tag="LND56_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6743943.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="LND56_002061"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="LND56_002061"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK6743944.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="LND56_002062"
     CDS             5117..6280
                     /locus_tag="LND56_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6743945.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="LND56_002063"
     CDS             6397..6807
                     /locus_tag="LND56_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6743946.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="LND56_002064"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="LND56_002064"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6743947.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="LND56_002065"
     CDS             9380..9664
                     /locus_tag="LND56_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743948.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="LND56_002066"
     CDS             9824..10060
                     /locus_tag="LND56_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6743949.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="LND56_002067"
     CDS             10057..10455
                     /locus_tag="LND56_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6743950.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3653 a         1762 c         2221 g         2840 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttc
//
LOCUS       DAFCLG010000089        10306 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFCLG010000089 DAFCLG010000000
VERSION     DAFCLG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.089"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            18..491
                     /locus_tag="LND56_002068"
     CDS             18..491
                     /locus_tag="LND56_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBK6743951.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="LND56_002069"
     CDS             500..727
                     /locus_tag="LND56_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6743952.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1363..1734
                     /locus_tag="LND56_002070"
     CDS             1363..1734
                     /locus_tag="LND56_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743953.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1990..2232)
                     /locus_tag="LND56_002071"
     CDS             complement(1990..2232)
                     /locus_tag="LND56_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6743954.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2264..3166)
                     /locus_tag="LND56_002072"
     CDS             complement(2264..3166)
                     /locus_tag="LND56_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6743955.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3179..3367)
                     /locus_tag="LND56_002073"
     CDS             complement(3179..3367)
                     /locus_tag="LND56_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6743956.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="LND56_002074"
     CDS             complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="LND56_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6743957.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3982..4875)
                     /locus_tag="LND56_002075"
     CDS             complement(3982..4875)
                     /locus_tag="LND56_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6743958.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4953..5876)
                     /locus_tag="LND56_002076"
     CDS             complement(4953..5876)
                     /locus_tag="LND56_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6743959.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5925..6275)
                     /locus_tag="LND56_002077"
     CDS             complement(5925..6275)
                     /locus_tag="LND56_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6743960.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6308..7210)
                     /locus_tag="LND56_002078"
     CDS             complement(6308..7210)
                     /locus_tag="LND56_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6743961.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7203..8060)
                     /locus_tag="LND56_002079"
     CDS             complement(7203..8060)
                     /locus_tag="LND56_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6743962.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8394..9203
                     /locus_tag="LND56_002080"
     CDS             8394..9203
                     /locus_tag="LND56_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6743963.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9243..9740
                     /locus_tag="LND56_002081"
     CDS             9243..9740
                     /locus_tag="LND56_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6743964.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10125..10198)
                     /locus_tag="LND56_002082"
     tRNA            complement(10125..10198)
                     /locus_tag="LND56_002082"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10162..10164),aa:Ile,seq:cat)
BASE COUNT         2985 a         1950 c         1767 g         3604 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1141 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     1201 acagtgatgg acaattcttt tttattttta acattatttt gataataatg attatttatt
     1261 taaaaataca ttagataaca tttgttacga cacgaaaaag cttcacaaac aaatatcgtt
     1321 attttaaaat gagggcgaac tcacacaatt aggaggaata gtatgccatt tattgtttac
     1381 ttttttcttt cattactcgt gatctatatt ccattaccaa catttttcct gctcgtcaat
     1441 cgtctagcag agcaacatga tacgattgct gctcttacga aactttgttt acttctattt
     1501 gtttgtctag attactttac gagctattat tggctaaaac agacaagagt aaaaaagaga
     1561 tattatttct tccttgtctt tttcaatctc tttgagtttc tgatccattg ctttttactt
     1621 catcagtatc atacgatcca tttgtggatc atctgggttt tcaaagtggt cgtattattt
     1681 tgtatgttca cctttcctat ctcaagaaaa tttagggagg cattgtttta ttaaagaagc
     1741 ttctctgtat caataaagac acatttaagg aaaaactttt aaaaatctaa ttgggaattt
     1801 gcccgcataa ataagaggct gtgacaaaag tcttgtcaca gcctcttatt ccgtataaac
     1861 agtgttcaaa agcttgcatc ttcgataata tgcccaacca aatgaaaaat atgagaagct
     1921 atttttgttt ggctgttctt attacttgat gaagaacagc tttttcacga cctcttttta
     1981 aaagtctttt tacagttttc gagcaaagaa tgatacgatt agtactaaaa tgatggcacc
     2041 aatcaatgat ggcacgattg ccattcctgc aatacttggt ccccaagaac ctaataatgc
     2101 ttgaccaagt gctgatccaa ctaaacctgc tacgacattt gcaataatcc ccatcgaaga
     2161 acctttattc gtgattgctc ctgcaattgc tccgataatt cctccgacga taagtgacca
     2221 aataaatccc atgtttcgtt cctcctaatg ttttacttcc ttattactga acccgtggtt
     2281 ctgtattttc ttttactttt tgtgcgccgc ttccagctgc tgattttact ttatctgttt
     2341 gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt tactttgtct tgcaccgttt
     2401 cttgatcttt ttcaaattct tcttttgttt tgatgtccac tacgttagcg ttgacttcga
     2461 tcacttctaa atgtgtcata ttttttactt ctttgccgat tacttcttgc atttcttcaa
     2521 agattttttc gatatctttg ccatattcca caatgacatc catatcaact gctacttgtt
     2581 ttttaccgac ttctgtttcg atcccagccg ttacattatc tgtattcaca agttttcctg
     2641 ccacattaga gaagaaacca ccgtttgcag ataataaacc gtctacttgt tcaattgcga
     2701 ttccgatgat tttttgcaca actttatctt cataattcag ctcaccgcgc attgaatctt
     2761 cctgttcaga atcatgcttt ccttggttat ttgtatgatc agaaccaagt tttccttgtc
     2821 cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc atggtcagaa ggatgttttt
     2881 gctcttccac aggtttagaa ggatcgattg catcatgttg ttctggaact ttagattctg
     2941 gagttcctgg atgatgaggt gcatgctttt ctgcatcatg tttcttctct tctactcctt
     3001 gttgaacgtt agggactact ggatctggat gtgcagatac tggtgctgtt ttgttctttt
     3061 catcgttcac accatttgat tggtttggtg tcgttgggtc tggatgtgca gacactggtt
     3121 tttggttgtt cattttattg ttattatttt tgttattgtt attcatggat gtttcctcct
     3181 aagatttaaa atagtgatct aagattttcg tttgtttcag ataaaaaccg atagcgacgc
     3241 cgatcgctgc catgatcaag atcaataagg tcttgaaaaa gcccacggtg atccataaaa
     3301 cggctaatat caatccaagt accccaaaga tgatagggag cttgtatgtc gaaaatagtt
     3361 ctttcatttt tggtcccccc ctattctact cgtttgcggt cattgcttcg tttagctggt
     3421 tcttcaaagc ctgtgtattt caccgatact ttcactggct cttttgttcc caatatgtgt
     3481 tgtacttcat tttcgatttc tttcatcaat gttcctgttt taccaatgag tgctgaagtt
     3541 cgtttcaact ccccttttac attgacggcg atcttgtttt tcgttgcttt gacatttact
     3601 tttggtgaat ggatgaattc atcttcatgc aagtgagaac gaaccattcc ttcaattgct
     3661 tttttatcca acgtcaactc cccatcacca tgtttcagta gaaagtttcc gattgttttt
     3721 ggataaaaca ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc aacggcaagc
     3781 caaaatacat attgttgcac gtatgaatta gtgatggaaa agaatcgaaa cttctctagc
     3841 tgaaaaggca atggataggt tgcacttaat agtatcgcaa acaaaagcac agatagcaaa
     3901 agtaatgttc cgatcactcc gatcgttttt acacctttat tcattttcgc acctcctcat
     3961 aggggtgaaa cgatcaactt cttataagtt gatcgcttct ccaccagtta ctccgtaaat
     4021 ttgggctgta acataacttg ctttattcga tgctaagaaa acataaactg gtgctaattc
     4081 tcctggctga cctgcacgtt ctagtaaact ttgctgacca aattcaggga tcgaatcaga
     4141 aggttgtcct tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta caccatttac
     4201 acggattcct ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag cgattgaagc
     4261 ttttgttgct gcgtaatcta ataaatgttc gcttggattg aatgcttgaa cagaagttgt
     4321 tgttacgatt gctcctcctg ctgggatatg cggaacagct tctttcacta aagcaaacat
     4381 agcgatgatg tttatcataa atgtgtcttt gacttgctca agtggtaact cagcaataga
     4441 ctgacaagaa atttgctgtc ctgcgtttag taccatcgta tctagcccgc caagttcact
     4501 cacggcttta tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa aaggtagtaa
     4561 gacaatctta cgtcctgcat ctttcacgta ttttgctact tcctctgcat ctttttcttc
     4621 tcctgggaag aattgaagag cgatatctgc cccttctcta gcaaatgcaa tcgctgctgc
     4681 acgaccgatc cctgaatctc caccagtgat tagtacacgg cggttttcta gttgattatt
     4741 gccttcatag gattcttctc cacaatcagg aacaggaatc atattctgct gtaaagctgg
     4801 tgtatcctga tcttgtttag gaaatttctc cgtatggtac aagtttcgcg ggtcgattaa
     4861 ttttggttct gtcattgctg tattcctcct agtttttaaa ttgtattgcc aaaacaaatt
     4921 tctttgtttg atacctgttt taaatagatt ctttaatttg tataatcttt ccggtataaa
     4981 tggtaaccta aaactactgc tgctatcaaa gagccaaaga ctacgagtat aaagcctaga
     5041 gcagaacttt tacccggtaa tcctcctgta ttcattcccc aaatccctgc gaacaacgcc
     5101 ggaacggaaa tgattaatgc agctgaatct agatatttca tgatatgatt tagattattg
     5161 tccatcatcc cgttgaatag atcaccaata ctctctaata aatctcgata gatctcgatc
     5221 attttttctg tttgtcgctg tctcaaatgg acatcataga gtagctgttc atcgtctaaa
     5281 cgtttgatga aagtttcaga attccataat tgatccattg tttcccgttg atctcttaag
     5341 gtatgatcga tatatacgat gtctctttca agatctgcct gtttatacag ttcttcattt
     5401 tccgttgttt tacgagctgc tttatccaaa gcgtcaattc gctttttcat ctctcttagt
     5461 tctttcacgt aatgggtgta aatatctaat aatgtatacg tgatgatctt ttcaaacgag
     5521 tttaaatgat ttccatgctt tgtcagcagc cgatccacaa attcggtttt tctatcgata
     5581 caagtaatca agagattatc ggaaagtaca aaagatattg gcgttaattt tttttcgata
     5641 cgattatctg actgatctag attaatcact accaaagaaa atggatgctc tagtttcgtc
     5701 tcataaaggc gctccatccg agaaacttct tccggatatt cggttcctac gaaaatatca
     5761 tcaggaagct cgaattcttc gattgcttgc tcaatctctt cgggctttgc actatcaagt
     5821 acgagccaat tatatttttt ttcatctgtt tctattacat tcttttcttc atccacaaag
     5881 tagtatctca atgtatacct cctttttgga ctttctcgaa actattagct gaagaagcag
     5941 ttctttaatt tgatattata atcgagtact gcgatttcat tttctaatga aaagctgtca
     6001 agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat aattttgctc taatttagat
     6061 gtatagatgt ccaataatgg atgctcgatt ttagtcgcat attctccaaa ttccgaaaca
     6121 aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct tagaaatgga
     6181 tcggaatcat tgataagcgc gagtgcgatt tttgtatttt ccccaacctt ttcaattaac
     6241 gtctctactg catttttaga cattgtaatt tccattccta ctccccctct tcagttgttg
     6301 tcttctatta tttaacaact acatctgttt ttggttgaac ttttgcatat tttggatatt
     6361 tagggaattt agctacacaa cggaaagccg tcatccctgc atatttttcg taatatttct
     6421 tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa tggataatgt
     6481 aaggattgtt gcgagtttct tcgtattcac gttcacctgc tgctgttgga tgtttttttg
     6541 cttttgccat gatatagcct tgtgcattcc aacgcggatg aagtggtaac caacggtcat
     6601 gtaagatagc atttaatgca tcttggtcat ggaatcgtaa tttttctgga ttatgttcga
     6661 tgaaatctaa tactttttgc gtaatatttt catctaacca ttttttcaca ttgatcagca
     6721 tcaaacctga gttgaaataa cgcattgatt tagctgaaat tttcattttt tccagtcgtt
     6781 gatggaaccc agcgtcttca acagctgcta cgattgaatc accgaagtca agacgccata
     6841 atttagagat atcttgtaaa gcgatcatgt cgcaatccat atataagata cgttcaactt
     6901 ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata agctgtttct ggaatacgat
     6961 cgctgattac cacatttgta aagaattctt tattgatttt caagaattcc acatctgagt
     7021 tcattcgtgc gtttttcact gagaagcgta aaagctgttt actttcaaaa tcgatatcat
     7081 cgtcaatcac atagaaccga acaaatttag acgggttgca attatctaat actgagacaa
     7141 ataatgcagc taaatgtggt acgaattttg tgttacaact tgcgactact gctatttctt
     7201 ttttattcat tgatctcgcc tactttcgtt agagtactgt gtgctaattt ttttaagtat
     7261 aaattggtat acggatgatc tttcaacgta ttccaaggtt tatcatgtcc tgtaaagtga
     7321 acgatcgaag gtttttcatt tccttcttta tactgacgtt catatttttc atttggtgct
     7381 ggatgacgtt caaaaatcag cgaagtctgc atgttccatt ttggatgaag ttgttcccaa
     7441 tcttcataaa gtacagcatt caaagcatct tggtcgtgat agatgattcg atcgccattt
     7501 tcactgaggt aatggatagt tttttctgta atttcttttt tattccattg atcaatgtca
     7561 atcaccatca ctcctgaatt gaagtacaag tcatcggaat cgattcctaa tctttccaaa
     7621 gctttcactt ggcctggatc gataatagcg ccgattgttt ttccatttaa cggctcatca
     7681 tatagtttta cgatatcgtc tagtactaac acatctgaat ctaagtaaag gactttctta
     7741 taatcctctt tagctaataa gtttggtaaa gaaattctta agtaagcggt agttgtgata
     7801 tggtcactga ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa ttggatactt
     7861 gcattacttg aatatttatt taccgtttct tccaatcctt ctttactata ttctgataga
     7921 ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat tgcaattttc taatgcagtt
     7981 gcgatcatca cgctcaagta aggagcataa ttttcgtctg aagcagttac cacaggaact
     8041 acgccgtatc tcttctccat caaaatcact cctttttcta atctttttct aacctgttct
     8101 acatctagaa tattcctcat ttggttttaa agcaaaatat atgctttgtt ttcattgaat
     8161 attttaaaat ttttttatca caaaacgcat cggtaagcgc taactattcg tttttcatgg
     8221 ctttttattc aaaggtattg accaaaaact actatatata gccacctttt tcttaagaat
     8281 gttttttgaa aaaattcata atgacaaagc aagttagttt cattttttta aattctcatt
     8341 taatcttaag gtaacaggaa aatagatatg taaaaagaaa gtaggaagat aaaatgaaac
     8401 tagcagcgat tgatttagac ggtaccttgc ttgatcgaaa tggaaagata ccagaagaaa
     8461 atatccaagc acttcggaaa tttgatgaaa atggcggaat cgtaaccgtc gctaccggaa
     8521 ggaacagcat ttctgctaaa gatatttttt ccgaattagg aatcggtggc tatttgattt
     8581 cttccaatgg ttcactgatt gctgaaatga aggatggaaa aatggcgaaa gttctcagac
     8641 gttcaaaaat agatagcgat acactgagaa aagccttctt ctatgctcaa gaagcgaaaa
     8701 tatccatcat tgctagccga gaaacccaag atgatcagct gacattcgat gaatcaaatc
     8761 tagtcaaaga tgacccttat tatgaacatt tcaatttaca gaaccattct tttgatcaaa
     8821 ttttaaatat gttggaagat ccttctctta gctatcttaa attagcatta acagataaga
     8881 gtgaagaaaa actacagaag ctacaaagag atctgcaagc agatggtatc caatcagtat
     8941 tctctgatcc gcatttctta gaaatcacgc ctacaggtat cactaaagct cattcacttg
     9001 tttttctggc ggattacctt ggaatcgaat cgagtgacat catggctttt ggcgatcaag
     9061 aaaacgacat tgctatgttg aattacagcg ggttgagtat tgcgatgggg aatgcacagc
     9121 gacaagtaaa agaaattgca aaccaagtaa cactgacgaa tgaagaagct ggtgttgctt
     9181 atattttaaa aaacttctat taaaaagaat aaaatgacga tatgaatgta ggaggaaaga
     9241 aaatgttagt tattattgat atgcaaaatc atattcttga tccaaacagc gaattttata
     9301 ttccagagtc agatcaattg gcagaacgta ttgctcaaag attagctaaa gcacgccaaa
     9361 ataatgaata tattctcttc acacgggata taccagttga actgaaggac aaagaagaag
     9421 aacgagaaga tttgcaattg atccctcttc tttctccaca agaaaatgaa cgagtgatta
     9481 aaaagtatta ctttacgatt ccacctgaaa ctttagctga aatccaggaa actttatttg
     9541 aaagcaaaga agaacaaaaa gagatcgaag tagtcgggat tgaaacaaac ttgtgtgtac
     9601 tctctaatct gataggcctg cagagtgctt ttcctgaagc agactttttt gttgatccaa
     9661 tgttagtctc cagtagaaaa cacggagaat ccgctttaga gctgttgaaa gatttcaacg
     9721 tctctatcgt cgaatcataa aaatatctat cttcatttac gatatttaac acacaataaa
     9781 aaaagcacgc agaactgttt tagtcctacg tgctttttct atcaaagtag attggctttt
     9841 ttatgacaca gagtgaaacg gcaggctcac gacctctttc ttaaatggtg ttcaatcagc
     9901 tgaccctcgg tattagctta acaaacggca aaatatgagg aactattttt ccgtttgtta
     9961 agctaatact caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tattcaaaag
    10021 cttgcatctg cagtaataag aacagccaag taaaaacata agaagctgtt tttacttggc
    10081 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttatgtacc ttgtaggact
    10141 cgaacctacg accggacggt tatgagccgt ctgctctaac caactgagct aaaggtacat
    10201 cataaataaa atttattttt gttacttttc aactatagca cgaatgacaa actctcacaa
    10261 gtttcaaaat ataagtaaaa taatattgtt attcttattt ctttta
//
LOCUS       DAFCLG010000090        10243 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFCLG010000090 DAFCLG010000000
VERSION     DAFCLG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.090"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            160..390
                     /locus_tag="LND56_002083"
     CDS             160..390
                     /locus_tag="LND56_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBK6743965.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            487..1788
                     /gene="murA"
                     /locus_tag="LND56_002084"
     CDS             487..1788
                     /gene="murA"
                     /locus_tag="LND56_002084"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6743966.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1801..1977
                     /locus_tag="LND56_002085"
     CDS             1801..1977
                     /locus_tag="LND56_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6743967.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2112..2408
                     /gene="yidD"
                     /locus_tag="LND56_002086"
     CDS             2112..2408
                     /gene="yidD"
                     /locus_tag="LND56_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK6743968.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2592..3059
                     /locus_tag="LND56_002087"
     CDS             2592..3059
                     /locus_tag="LND56_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6743969.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="LND56_002088"
     CDS             3144..4028
                     /locus_tag="LND56_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6743970.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4142..4378
                     /locus_tag="LND56_002089"
     CDS             4142..4378
                     /locus_tag="LND56_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBK6743971.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="LND56_002090"
     tRNA            4466..4546
                     /locus_tag="LND56_002090"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="LND56_002091"
     tRNA            4553..4624
                     /locus_tag="LND56_002091"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
     gene            4749..4919
                     /locus_tag="LND56_002092"
     CDS             4749..4919
                     /locus_tag="LND56_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743972.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5139..5846
                     /locus_tag="LND56_002093"
     CDS             5139..5846
                     /locus_tag="LND56_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6743973.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5906..6550)
                     /locus_tag="LND56_002094"
     CDS             complement(5906..6550)
                     /locus_tag="LND56_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBK6743974.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="LND56_002095"
     CDS             6677..6964
                     /locus_tag="LND56_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743975.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="LND56_002096"
     CDS             7072..7392
                     /locus_tag="LND56_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6743976.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="LND56_002097"
     CDS             7473..8786
                     /locus_tag="LND56_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6743977.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8783..9502
                     /locus_tag="LND56_002098"
     CDS             8783..9502
                     /locus_tag="LND56_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBK6743978.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="LND56_002099"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="LND56_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK6743979.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3415 a         1693 c         2187 g         2948 t
ORIGIN      
        1 ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa tgtaagggga
       61 ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta ttatcatttt
      121 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
      181 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      241 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      301 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      361 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      421 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      481 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      541 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      601 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      661 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgatgct
      721 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      781 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      841 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      901 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      961 atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa
     1021 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
     1081 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1141 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1201 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1261 tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1321 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1381 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1441 gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1501 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1561 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1621 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1681 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt tgaacgtgta
     1741 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1801 atgagtaacc aacgatatat catattaact ttgataaaga ttttagtggt gatcttattg
     1861 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1921 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1981 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgacaaaa
     2041 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     2101 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2161 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2221 caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2281 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa ttaacgcgaa
     2341 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg agtagcactg
     2401 agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg tgagttattg
     2461 ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg ggtagcactg
     2521 agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tatggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4741 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4801 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4861 aggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4921 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4981 aggttgaaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     5041 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     5101 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5161 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5221 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5281 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5341 catatgttgc gcgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5401 ttatggaatc gcaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5461 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5521 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5581 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5641 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5701 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5761 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5821 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattcctt acttagaaat
     5881 aaaagagaat cagaatcacc tgtttttata tatgatcagt taccagttgc tcgtgataag
     5941 aaacaggacc ctcttcaaat tggacttgtc cgttcagcag ctcaatgatg gattctttaa
     6001 agtggtcagc acttttttca tctacagcac agatgacgct aacttgatca gtatattgga
     6061 tttcttttat aaaaaaatgt tggagttcaa gataattctg taattttcct agttgcggat
     6121 acgtgatcat cacagagatc tcctgttgca aggtaccttc tacgattcca atgtcagaca
     6181 atgcttgaga gacagcatga gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc
     6241 ctccaaaata tcgagtgact actgcagcta cattgatcag ttgatttttt ttgaggactt
     6301 ccaacatcgg tacgccagcc gttccacttg gttctccatc atcgctgctt cgctggattt
     6361 catttttttc tccaatgatg aaggcgctgc agttatggtt cgctttccaa tgttcttttt
     6421 ttatttcttg aatgaaactt tttgcttggt cctctgattc tattcgtcta agagaacaga
     6481 taaagcgtga ttttttgatc tcaatctcac tttgtccatt ttcttttatt gtacgatagc
     6541 gctcgatcat cttgtcacct ctttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag
     6661 gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc tcaagttcgc
     7261 tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt agacgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga atgaggacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat cggcgtttac
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     9481 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt ttt
//
LOCUS       DAFCLG010000091        10058 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFCLG010000091 DAFCLG010000000
VERSION     DAFCLG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.091"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            201..680
                     /locus_tag="LND56_002100"
     CDS             201..680
                     /locus_tag="LND56_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743980.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="LND56_002101"
     CDS             713..2941
                     /locus_tag="LND56_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6743981.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="LND56_002102"
     CDS             3070..4713
                     /locus_tag="LND56_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6743982.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="LND56_002103"
     CDS             complement(4786..5331)
                     /locus_tag="LND56_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBK6743983.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="LND56_002104"
     CDS             complement(5354..5839)
                     /locus_tag="LND56_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6743984.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="LND56_002105"
     CDS             complement(5829..6641)
                     /locus_tag="LND56_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBK6743985.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="LND56_002106"
     CDS             complement(6780..8312)
                     /locus_tag="LND56_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK6743986.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="LND56_002107"
     CDS             complement(8421..9452)
                     /locus_tag="LND56_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6743987.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3355 a         1851 c         1923 g         2929 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aagttgtttg cttattgatg gattggataa attaaaccat cacaacgagt
     9961 ggaaattata tgaaattttt cataaaaatt catatagaaa caaatgacat tttattagaa
    10021 tatgactttc attaacataa atgttttcaa atttcaat
//
LOCUS       DAFCLG010000092        10022 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFCLG010000092 DAFCLG010000000
VERSION     DAFCLG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.092"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..722)
                     /locus_tag="LND56_002108"
     CDS             complement(<1..722)
                     /locus_tag="LND56_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6743988.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="LND56_002109"
     CDS             complement(727..1494)
                     /locus_tag="LND56_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6743989.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="LND56_002110"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="LND56_002110"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBK6743990.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="LND56_002111"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="LND56_002111"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBK6743991.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="LND56_002112"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="LND56_002112"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBK6743992.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="LND56_002113"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="LND56_002113"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBK6743993.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="LND56_002114"
     CDS             4894..5211
                     /locus_tag="LND56_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6743994.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="LND56_002115"
     CDS             5211..6917
                     /locus_tag="LND56_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6743995.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVSEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="LND56_002116"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="LND56_002116"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK6743996.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8588..8761
                     /locus_tag="LND56_002117"
     CDS             8588..8761
                     /locus_tag="LND56_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HBK6743997.1"
                     /translation="MKLPSLLKCGKWYVLTGKEWICHSDLELSAFEWEFLQSLDVEIR
                     ETIHFEVNYLPFQ"
     gene            8782..9681
                     /locus_tag="LND56_002118"
     CDS             8782..9681
                     /locus_tag="LND56_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK6743998.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3163 a         1690 c         2007 g         3162 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttagtgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gt
//
LOCUS       DAFCLG010000093        10021 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFCLG010000093 DAFCLG010000000
VERSION     DAFCLG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.093"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(485..709)
                     /locus_tag="LND56_002119"
     CDS             complement(485..709)
                     /locus_tag="LND56_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6743999.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            900..3677
                     /locus_tag="LND56_002120"
     CDS             900..3677
                     /locus_tag="LND56_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6744000.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3758..4177
                     /locus_tag="LND56_002121"
     CDS             3758..4177
                     /locus_tag="LND56_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBK6744001.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4189..4659
                     /locus_tag="LND56_002122"
     CDS             4189..4659
                     /locus_tag="LND56_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6744002.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4676..5455
                     /locus_tag="LND56_002123"
     CDS             4676..5455
                     /locus_tag="LND56_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6744003.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5442..6263
                     /locus_tag="LND56_002124"
     CDS             5442..6263
                     /locus_tag="LND56_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6744004.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6280..7329
                     /locus_tag="LND56_002125"
     CDS             6280..7329
                     /locus_tag="LND56_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6744005.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7342..8346
                     /locus_tag="LND56_002126"
     CDS             7342..8346
                     /locus_tag="LND56_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6744006.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8979..9998
                     /locus_tag="LND56_002127"
     CDS             8979..9998
                     /locus_tag="LND56_002127"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6744007.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1651 c         2094 g         2914 t
ORIGIN      
        1 aatttgttct tgcaatcttt tttgctacgt aatttttgta aaaaagtggg ttgagagaaa
       61 tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa atatttattc gtttattgtg
      121 ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg cagattctat
      181 caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagctact tgagattttg
      241 atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct tgttttcgta
      301 atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg tgacaacagt
      361 acctctttac tctctgaaag catcataact cgtttttcct cattatgcca ataaattgtt
      421 gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt gaaatctgtg
      481 aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag atgaataaaa
      541 atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt tctgtcatag
      601 ccatataaag caaaggcaaa aatacaacta cagcgagata agatccgatg atttctcgaa
      661 aagttttcca ggaagtatgt ttcctgtttt tatatttatc cgatttcatt gaatagtcat
      721 agcctcctta ttctttcata tgttgaaatt ccagtataaa aactggaagg aactgtacgt
      781 tttttagaat aaaaattata agggataagt aggctgctgt ctataataag atatattttt
      841 caattcttta ttaaagattt tattgacaaa atgaatccgt tttctatgat ggaggagggg
      901 tgaataagtt gttgaagaag gaaattttat gttacttaga gaaccagaca gcctttttcg
      961 atttagaaca tatgaacgag gtctttacag cacaaaggtt agctgatcat tttggggtaa
     1021 aaagaaacac agtcagtcat tatttgaacc agttaaatga tgagcaacta ctagtcaaaa
     1081 tcaattctcg accagtgatt tatttccaca aagaagcttt tgaaaaacaa ttttttaagt
     1141 tacgcacgaa tttttatttt tctatcgatg atctcaaaga ggaacagcct tttttcgcgc
     1201 aaccaaaaga cttattctct tttatgatcg gatacgacgc cagtttgaaa gaaagtatcg
     1261 aacaaatcaa aacagctctt tactatccag atggcggatt accacttttg attacaggcg
     1321 aaagtggcac aggtaaaagt tatttagtta atttggtcta tcagtattgt ctgcttcatg
     1381 atctgctaga agattctgca ccattcatca cttttaattg tgcacaatat gcagataatc
     1441 ctgaactttt gactagcaat ttgtttggac atgttaaggg agcgtacact ggtgcagaag
     1501 aaaataaaaa aggggctttt gaagcagcag atggtgggat cttgttttta gacgaggtac
     1561 atcgcttaac accagaaggt caggaaaaac tgttcaccta tcttgatcaa ggtgtgattt
     1621 atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt tttgctacga
     1681 ctgaagagat tactagtcat ttcttaacaa ctttcattcg aagaatacct atccgaatcg
     1741 aactacctgc acttaatcag cgtagtcgta atgaacggct agaactgatt tactcttttt
     1801 ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt cagtggacca gctttgtcac
     1861 ttttaacaag tcagaaatta tccggaaaca taggagagct gaaaaatatc gtcaaagttt
     1921 cagcagcaaa agcatatgct gaacagcgtg aacagcctga aatccatgtc acgatccatc
     1981 atttgcaaaa agaactactt gcgcagccta ttactaaaaa tacagcacag caaggaatct
     2041 atattacgca agaccagacc ttagagcaat taatggagaa gcaacagccc gaacagcagc
     2101 gaatcgttaa aagttttgag caaatcctaa ttgatttcaa gaaaaataac tgtctgctat
     2161 attcttgcga aggaaaatta aagcaagaag tgattcaatt atttgatttt ttgctgtttg
     2221 agaccagtag acaagaaaaa cacgagctgc tggtttattg gacacaatct atccgagaga
     2281 cctttcgaca aatggaaact gcctatcaaa tcaaattcga tggaaacagt gtctatgcct
     2341 tgagttacta tctctatcag cgaagcacta tcaaatggtt accggaagaa catgatatta
     2401 tccaactaat caaagaatta gaacaacaag tttctcagtc ttatccatct agttatcact
     2461 atgtccaacg aattctggaa ttaagtaaga tcaaattaga tattgaagac acaagtatgg
     2521 atcggatttt actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa aaacgactgc
     2581 caaaagcgat gattgctgct cacggttatg caactgctag cagtatggca aatgtagtaa
     2641 atcgtttatt acaagaggat ctatttgaat catttgatat gccactagat gtcacaccac
     2701 agcagattgc ttcagaaatc cttgattatt gcgaaaacag cgatgtttca aatggattga
     2761 tcatattagt agacatggga tctctgaaag aaatccatca gttttttaaa aaacagttat
     2821 ccgtaccgct tttgattttg aataatgtga cgacaccttt agcaatcaca gtaggggaat
     2881 gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga gactgttcga cagatccagc
     2941 cagaatggcg actgctctat ccagaggaaa acaaacccaa agcactcatt actacttgct
     3001 tcactggaat cggtacggcg gtccacttaa gtgaactgct agaaaaaagc ttacctacgg
     3061 cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt aaaagataag aaaaacagtg
     3121 acccattatt ttccatatac gaagtcgtag gaatgattgg caccacagat cctgatatca
     3181 ccaatatcga ctatctttca ttagaagacc tcatctcggg ggaaaagatg tctgtactag
     3241 ctgaatggtt ggacactact atgaatgctt cagagaagga aatattcaat cagcgcatca
     3301 ttcgtaattt ttcattggaa aaagtactgg attccgtgac tattctagat acagagaagg
     3361 tgatgggaga aatcgatcat tttatgagag aattagaggt gcagttgaat cgaagcatca
     3421 gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa cgattgatcc
     3481 gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt caaaaagaag
     3541 ggttatctgc tattaaatct gcgtttagtg tcattgaaga gcattatagt gtcatgatcc
     3601 ccaattctga gattgcctat atctatgata ttttgagtga agatacagag tttctctcga
     3661 tagaagaaga attttgaaca taagcaagtt gttgccacta attttggcat gcaacttgct
     3721 tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg gatagagaaa ttatcttagc
     3781 ttcccatgga aaatttgctt cagggatatt agattcatta gagctgatct atgggaaaaa
     3841 tcattctatt actgtattcg attgctatac tgatgagaat tttgatttag cggaaacagt
     3901 aaggcagctt tttatgaggt acaaagataa agaaatcatt gttctgacgg atctttttgg
     3961 cggaagtgtc aacaacgaat tcttacaata tatcaatcgg gatcatgtct atctcgttgc
     4021 cggattgaat ttgccactat tgattgagct agtatctcgg ttggacgctg aagtagagac
     4081 aactgttttg atccagcagg tgctaactga ttcaaaaaaa atgatccagt tttgtaacga
     4141 gcggctcaat cagaaaatag aagaagacga attttaggag gaagaaaaat gattgtattg
     4201 acaagagtcg accatcggtt attacacgga caagtagctt tttcatggac acaaacaata
     4261 ggcgcagact gtattttgat tgctaatgat gatgtaccta cgaatgagat acgaaagaca
     4321 acgatcaaat tagccaagcc acaaggagtc aaactagtga tcaaatcaat tgatgactcg
     4381 attgccgcgt tgaaaagcgg ggtgacagat aaatataagt tattcatcgt agtggaatcg
     4441 atcgaggatg catataagct ggcagaagcg tatccagaaa tcaaaaccat caacttagga
     4501 gggatcaagg caaaacctgg tactcgcagc atcggaaaag cggtaaatat tttaccagaa
     4561 gaggaggaac gcttgaaacg attaattgac aaacaaatag aagtagaaat ccgtcaagta
     4621 ccagcagata aaaaagtgga tgtaagagaa gttttgtaag aaaggaagaa cctcaatgat
     4681 tgtacaagca gtcttattag gaattgttgc atttgtcgca caatcagaat atgcactagg
     4741 gacatcgttg ctttctcgtc cgattgtgac ggggctgttt acaggaatcg tgttaggtga
     4801 tgtaaaaact gggatcatta tgggcgcaac tttagagtta gcatttatcg gatcgttctc
     4861 agtaggtgct tcgattccac ctgatgttgt gaccggtggg attttaggga cggcttttgc
     4921 tattacagct ggagccggta cagaaaccgc gcttttacta ggtttgccga ttgctacgtt
     4981 gacattgata ttgaaaaata tttacttagg gcttttgatt ccaattatga accataaagc
     5041 agacaattat gcagaagaag gaaattataa aggagtcgag catatgcact tgcttgccgg
     5101 tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt tcttatatgg tgggaagcaa
     5161 tacgatcgga aatctattaa atatgattcc tgacttcgtt caaaaggggt tgtcagtagc
     5221 tacagggttg attccagctc ttggttttgc gatgcttgct cgtttgctga tcaacaaaca
     5281 agttgcacca tattttttct taggttttgc tatagcttct tacttagaga ttcctgtgac
     5341 cggagtagct atttttggtg cgattttagc agttgtggta gtcaatatta tgaatgtccg
     5401 tcaactacca cttcaaacaa ctgcagggga ggtagaagaa gatgaagaat tctgagatca
     5461 aaaacagcaa aataacgaaa aaagagctaa atcacgtatt ttggcgttct tttcaaatgg
     5521 agttttcttg gaattatgaa cgtcagatga atatggctta tgtttatgca atgattccga
     5581 ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc agctttgaaa cggcatctag
     5641 agttttttaa cacgacccca catatcgtta cactgatttt aggaatcacg gctgcaatgg
     5701 aggaagaaaa taaggatgat cctgaatttg atgtgactgc gatcgatagt atcaaaacat
     5761 ctttgatggg acctcttgca gggttaggag attctttctt ttggggaact ttgcgattga
     5821 ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa tattttaggc cctatcttgt
     5881 tcattttgat tttcaatatt ccgcacattc tgtttcgcta tctagcaaca ggttggggat
     5941 atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa cggtatgatg gaaagtttga
     6001 cattaggcgc gtctattatt ggactgatgg tcgttggagc aatgactgcc acgatgatca
     6061 ccatcaatat tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt caaagtattt
     6121 ttgatgatat cgttccaaat atcttatgtc ttggagcatt tggcatagtg ttttatctat
     6181 tgaagaaaga agtgaaacca ttaacgatct tgatcggact agctttcttt ggaatttttg
     6241 gttcgtggat agggattttt taaaaaagga gcagagaaaa tgtttacgat gcaagattat
     6301 atctatgaag aaaaggaagt actaagtacg atcttgaaaa agaatgattt ttcgacaaga
     6361 gaacatatga aaaaaacagt gaatttactg atcttagcaa cgggttcatc ttataacgct
     6421 tgtttagcag cgaagccagc attggaatct tatggtgatc ttactgtcga tattcaagaa
     6481 cctttttatt ttttcaatta tggaaaactt tctccttcaa tcgatactgt gattgctgtg
     6541 tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa aaatgataca aaaacaaggg
     6601 ttacctgttg ttgctatcac aaatgacgtg caaagtccac ttgctttaga agctgcacaa
     6661 attattgatt taggtgccgg ggtagaatca gtaggcttcg ttacaaaagg ctattctgcg
     6721 actgttttac agttactact cttagggata ggaattggaa taagtaaaaa taaaatcagc
     6781 aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa taatcaacca tttaccagcg
     6841 attattcaaa aaacggaaga attttttgat ggatatcaaa gtttatttcg tttagcacag
     6901 cgtttcgtca ccattggata tggtccaaac tggggaacag ctaaagaagc agagacgaaa
     6961 ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt tagaagctta tatgcacggc
     7021 ccatacctgg aggcagatgc ttcgcatttg ctattcttta ttgaagggga aagtgtaaat
     7081 aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt acgttggaga gactctcacc
     7141 attaccacaa aaaaagcaag aaacgagaaa acacttggat tagcaataga atgtgatgaa
     7201 tatctgtctg tattagcgct tgtcgttccg tttcaactat tcgcttataa aaccgcagta
     7261 gctaaaggta ttgacttgaa caaaaagatt tttgaagact ttgatacagt cttgaaaagt
     7321 aaattataag aggtggaaat catgctgaaa ttcaatgaag aagaacaaat caaagcaata
     7381 aaaggcgcac tagctttacg tccgcaagta gaagaaatca tcgataaact ttatgcggaa
     7441 aaatttgacg ctgtctatta tctaggaatc ggtggaacgt atgcttcctc gatgcaagct
     7501 gtcacttata tgaatgggaa aagcaacttg cctgtctttg tacagcatgc tgcagaatat
     7561 tacactactg gaaacaaaag attaacaaaa gattctatcg ttattttgtc ctctgttacc
     7621 ggtacaactc aagaagtagt gaaagcagta gaacaaatca agaaagtagg agcgacactg
     7681 attggattta tcgataaagc gaacagtaag ttgtctcagc tatgcgattt tgtggtcact
     7741 tatccagctc ctggaacaga acaaatcaaa ttttttatgg cagcagatcg attgatgtat
     7801 ttgaatggag aattcgaagc ttattcagaa tactatgaac agctcgaacg ttatttacca
     7861 acagggttag tggaagcaga gaaaaaagca gatgcatttg gcttatcatt tgcagaaaaa
     7921 catcgacgtg atagtatgca ttattttatt ggagcaggga atcaatgggg agctgtttat
     7981 tcatatgcca tgtgttactg ggaagaacaa agctggcttc catcgaaatc tatccatgct
     8041 gccgagtttc ttcacgggac gttagagatc gtagaagaaa caaccccagt cacattattt
     8101 ttaggcgaag atgaacagag agtgttatct gaacgtgtgg ctaaactttt gccaaaaatt
     8161 tgcagcaatt atacattgat agacacaaaa gactatccag tagaaggtat cagtgaaaaa
     8221 tatcgcggac gtgttttgtc tttcttattg atgcatgtag tgacacaacg catcgatgct
     8281 catgtggagc agttgaactg ccatccatta gagatacgcc gctactatcg tcagtttgat
     8341 tattaggata gactggataa aaagaatata aaaaataaag agagcagcga taactgctcc
     8401 ctttattttt ttaaaatatt ggcatcaatt acgttataaa acacattttt tgcattatct
     8461 acagttcaac ttataatctg tttaatggga catgtgggtg ccaaccattt tttgttatgc
     8521 aatagtacta tttagttgtt gcatggatcc gtagtaaaag tccaagtaca acgagttgcg
     8581 ccagtttaca tagcgttttg tatccagaat gttgactaaa acaaaagttc ctatgtcaac
     8641 cgtcaacaga gacaatctgt ccgttgcagg acctgacaga tttagaaatg ttcaatctgt
     8701 ctcttatgta ttttaaatca atactcacta ggagaagttt cctaacctcc taatagtaat
     8761 gctggtagta acattgtcaa aactgcttcc tcttaggaac aagcaaagca taacgagtgt
     8821 aaaaataaaa tgtagactat tgtaacatat cattgattat ttcaagtaaa aacgataaaa
     8881 gaagtaaaca aacacttaaa aacaatggaa attagtccca actttaagaa atgtatacta
     8941 taatggaaat tatcaattat ttatttggag gaatatccat gaaacaaaaa atcgcagttc
     9001 ttggtcctgg atcttggggc actgctttag cacaaacatt ggcagaaaac gggcatgatg
     9061 tccgtatttg gggaaatgta cctgaacaga ttgatgaaat caataagtat catacaaatc
     9121 agcatttttt acctgatttg acgatcccag aatcgattat cggatataaa gaattggcag
     9181 aagcagtgga taacgcagat gctattttat ttgttgttcc aacaaaagcg attcgttcag
     9241 tcgcacagga acttattggt aaaatgaata ctaagccaat cattatacat gcaagtaaag
     9301 gactagaaca agatacgcat aaacgaatct cagaagtttt ggctgaagaa attccagaag
     9361 aaaaaagaca ggcaattgtt gtattatctg gtcctagcca tgctgaggaa gtggcagtac
     9421 atgatatcac aacaattaca gctgcaagta ttgatcaaaa agcagcagcc tatgtacaaa
     9481 aactgttcat gaatgattat ttcaggattt atacaaataa tgatgtgatt ggcgtagaaa
     9541 ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg cgctatccat ggattaggat
     9601 tcggagatga tgcaaaagca gcaatcatga ctcgaggatt ggcagaaatc agccgtcttg
     9661 gtgtcgcgat gggtgccaat cctctgacat ttatcggttt gagtggtgtg ggggacttga
     9721 tcgttacttg tacaagtgtt cattctcgca attggcgtgc cggtaacttg ttaggacagg
     9781 gacataagct ggaagaagtt ctagaaaaca tgggaatggt cgttgagggt gtggcaacga
     9841 caaaagcagc agtagagcta gctcaacagc taggtgttga gatgccaatt actcaaacaa
     9901 tctataatgt tttgtataat ggagaagata tcaaaaaagc agcaaaagaa atcatgttac
     9961 gtgacggaaa aatggagaat gaattttctc ttcgttaaat agatgatcaa tagttaatcg
    10021 t
//
LOCUS       DAFCLG010000094         9857 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFCLG010000094 DAFCLG010000000
VERSION     DAFCLG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.094"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LND56_002128"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LND56_002128"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK6744008.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="LND56_002129"
     CDS             complement(2260..2511)
                     /locus_tag="LND56_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBK6744009.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="LND56_002130"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="LND56_002130"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBK6744010.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="LND56_002131"
     CDS             3461..3679
                     /locus_tag="LND56_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744011.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="LND56_002132"
     CDS             complement(3968..4480)
                     /locus_tag="LND56_002132"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK6744012.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LND56_002133"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LND56_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK6744013.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="LND56_002134"
     CDS             complement(6933..7376)
                     /locus_tag="LND56_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBK6744014.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="LND56_002135"
     CDS             complement(7377..8165)
                     /locus_tag="LND56_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6744015.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="LND56_002136"
     CDS             complement(8162..9103)
                     /locus_tag="LND56_002136"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBK6744016.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..>9857)
                     /locus_tag="LND56_002137"
     CDS             complement(9280..>9857)
                     /locus_tag="LND56_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBK6744017.1"
                     /translation="VIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDPNGTH
                     QFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEIKDAQ
                     TVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVHDAKE
                     AENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
BASE COUNT         2695 a         2201 c         1742 g         3219 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagag ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactc
//
LOCUS       DAFCLG010000095         9848 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFCLG010000095 DAFCLG010000000
VERSION     DAFCLG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.095"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1030
                     /locus_tag="LND56_002138"
     CDS             <1..1030
                     /locus_tag="LND56_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6744018.1"
                     /translation="RLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYLVR
                     FLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMTDF
                     RFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIAFL
                     MGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVMYP
                     SYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIALST
                     YFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEGEL
                     IESLKNEI"
     gene            complement(1178..1543)
                     /locus_tag="LND56_002139"
     CDS             complement(1178..1543)
                     /locus_tag="LND56_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBK6744019.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(1690..2463)
                     /locus_tag="LND56_002140"
     CDS             complement(1690..2463)
                     /locus_tag="LND56_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6744020.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(2637..3497)
                     /locus_tag="LND56_002141"
     CDS             complement(2637..3497)
                     /locus_tag="LND56_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBK6744021.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(3497..4915)
                     /locus_tag="LND56_002142"
     CDS             complement(3497..4915)
                     /locus_tag="LND56_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6744022.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(4972..5421)
                     /locus_tag="LND56_002143"
     CDS             complement(4972..5421)
                     /locus_tag="LND56_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6744023.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(5866..6129)
                     /locus_tag="LND56_002144"
     CDS             complement(5866..6129)
                     /locus_tag="LND56_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK6744024.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(6228..7523)
                     /gene="rho"
                     /locus_tag="LND56_002145"
     CDS             complement(6228..7523)
                     /gene="rho"
                     /locus_tag="LND56_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK6744025.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(7520..8791)
                     /locus_tag="LND56_002146"
     CDS             complement(7520..8791)
                     /locus_tag="LND56_002146"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6744026.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(8956..9825)
                     /locus_tag="LND56_002147"
     CDS             complement(8956..9825)
                     /locus_tag="LND56_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBK6744027.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         2695 a         2121 c         1744 g         3288 t
ORIGIN      
        1 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
       61 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
      121 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
      181 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
      241 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
      301 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
      361 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
      421 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
      481 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
      541 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
      601 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
      661 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
      721 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
      781 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
      841 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
      901 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
      961 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     1021 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     1081 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     1141 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     1201 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     1261 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     1321 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     1381 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     1441 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     1501 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     1561 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     1621 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     1681 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     1741 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     1801 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     1861 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     1921 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     1981 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     2041 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     2101 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     2161 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     2221 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     2281 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     2341 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     2401 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     2461 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     2521 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     2581 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     2641 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     2701 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     2761 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     2821 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     2881 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     2941 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     3001 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     3061 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     3121 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     3181 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     3241 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     3301 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     3361 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     3421 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     3481 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     3541 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     3601 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     3661 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     3721 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     3781 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     3841 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     3901 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     3961 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     4021 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     4081 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     4141 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     4201 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     4261 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     4321 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     4381 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     4441 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     4501 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     4561 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     4621 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     4681 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     4741 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     4801 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     4861 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     4921 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     4981 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     5041 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     5101 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     5161 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     5221 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     5281 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     5341 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     5401 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     5461 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     5521 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     5581 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     5641 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     5701 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     5761 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     5821 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     5881 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     5941 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     6001 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     6061 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     6121 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     6181 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     6241 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     6301 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     6361 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     6421 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     6481 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     6541 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     6601 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     6661 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     6721 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     6781 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     6841 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     6901 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     6961 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     7021 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     7081 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     7141 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     7201 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     7261 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     7321 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     7381 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     7441 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     7501 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     7561 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     7621 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     7681 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     7741 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     7801 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     7861 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     7921 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     7981 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     8041 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     8101 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     8161 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     8221 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     8281 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     8341 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     8401 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     8461 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     8521 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     8581 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
     8641 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
     8701 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
     8761 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
     8821 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
     8881 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
     8941 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
     9001 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
     9061 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
     9121 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
     9181 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
     9241 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
     9301 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
     9361 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
     9421 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
     9481 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
     9541 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
     9601 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
     9661 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
     9721 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
     9781 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
     9841 aagttttg
//
LOCUS       DAFCLG010000096         9822 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFCLG010000096 DAFCLG010000000
VERSION     DAFCLG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.096"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            333..539
                     /locus_tag="LND56_002148"
     CDS             333..539
                     /locus_tag="LND56_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBK6744028.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(741..1742)
                     /locus_tag="LND56_002149"
     CDS             complement(741..1742)
                     /locus_tag="LND56_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6744029.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1747..3659)
                     /locus_tag="LND56_002150"
                     /pseudo
     CDS             complement(1747..3659)
                     /locus_tag="LND56_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            complement(3827..4333)
                     /locus_tag="LND56_002151"
     CDS             complement(3827..4333)
                     /locus_tag="LND56_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6744030.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4493..4933
                     /locus_tag="LND56_002152"
     CDS             4493..4933
                     /locus_tag="LND56_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6744031.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4959..6116)
                     /locus_tag="LND56_002153"
     CDS             complement(4959..6116)
                     /locus_tag="LND56_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744032.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6119..6475)
                     /locus_tag="LND56_002154"
     CDS             complement(6119..6475)
                     /locus_tag="LND56_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744033.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6773..7747)
                     /gene="sgrA"
                     /locus_tag="LND56_002155"
     CDS             complement(6773..7747)
                     /gene="sgrA"
                     /locus_tag="LND56_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBK6744034.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7943..8782)
                     /locus_tag="LND56_002156"
     CDS             complement(7943..8782)
                     /locus_tag="LND56_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6744035.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8967..>9822
                     /locus_tag="LND56_002157"
     CDS             8967..>9822
                     /locus_tag="LND56_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6744036.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTI"
BASE COUNT         2949 a         1966 c         1478 g         3429 t
ORIGIN      
        1 ctcattttag gttatcattt gtaattttat ttcacagttg atatgattgt tatgtaaaat
       61 aaaaaagttt aggatacatc gaatttttgg ggaaattctt ttgggggtat cctaaatctt
      121 ttctattata ttcccatatt ttctattatt ggtctagacg tatgatggta aactttgtac
      181 ctatctgatt gctgaatcat taattgctta tcattagcca tctttgtttc aagttcgcta
      241 gatgatcatt ttattcacat cctaacaaac ttgtcacatt tatagattct taatctattt
      301 atcatacaat tataaaaaag gaggtgctgc ttatgaaagc tatccatatt tctttgcttt
      361 ttttcgctag tgcattgatt ttatttgcta ttcatctttg ttcccagcct tatatcaata
      421 acaacggaat attagtggaa ccagcattct tttgcttacc actcggcttt ttgtcccttc
      481 ttgcctccgc aggcttcgga tttgttgctt tttttaaacg atcaaaacaa caaatctgac
      541 ttataaaatt tacgtaaaag agatcgtgac aataatcgtg tgatattccc aaatttcgaa
      601 taaaaggtgc cccttgccat ctctaactcc tcagtcacaa gttacaatat tcataaacca
      661 tgaaaagctt gtttattgaa attgctcctt gtgacttgga gctaagtgac agattacaac
      721 ctcttttgca tcataagact ttactccttg aaaaacatta ctaacttttg atatgtttct
      781 tgcgcttgtt tattgctgaa atcaaactga tattcatggt tcaaaacagc atttttctcc
      841 gcataatcta cttttgtgac tttcaccccc gctttctcaa atacagaagt tacttcttcc
      901 atttgtttcg taaacgtatt tgtatttcca tccgttaaga atgtgttttg cgtccaacga
      961 tccgcatgtc tgaccaaggc taaacttttg accttctctg attgttcaaa gtccttatta
     1021 tcaaagtaag cccatccaca tttttcataa agaaagctag agattttatc acttacttga
     1081 gtaagtgcag aaggatccaa cagaccagac aaagaaataa acttcgtaat ctgcttgttc
     1141 tccaaagtcg gatcaagatg atcagctttt ttaatttctt cattttgaag tgctaacaaa
     1201 tattctccac taatttgtgc acctgccgaa tttccaccaa tcgaaatatt cgaccaatct
     1261 gcttgatctt ttatctcttt tttagctgct tgaatggctt cgttcaattg gataactggt
     1321 gtaggatgtt ttgcttccgg tgatcgttca taatctatat ttacgatgat ttgctgcgtg
     1381 tctgaacata gatattccaa ataatcacga caatcatttt tatcccctcc tacatatgcg
     1441 cctccatgca tccagaaaag aatcggtaat tttttctttt ccactttcgg tgtgtagata
     1501 tccatattat tatttttgta ctttgaagga taggatattt ctttttttat atcgatttgt
     1561 tcgatttttt gctggaaatc atctggtcgg gcatatactt gttctttttc acttgtgtca
     1621 aattgttttc taacaagcaa gctaatcggc tttggtgtga tttgattaat caaaaataca
     1681 gtgactaaaa taactacgat gataactggt actgcaatca ctattcgctt gaaccatttc
     1741 attcatttac cctctctttc ttcgccaaat ccatgtgata atcgtgctaa cagataaaaa
     1801 tccacctgaa taaatgagaa gcgaatgttt cttttctcca gttttcggca acattctttt
     1861 tttgcatctg tctttttttg tactttcact gtcatctgaa tcggttgcgt atcatcactt
     1921 gattgatcct ctcgaggttc attctttccc ttattgtctg cattctctgt cggatcttgc
     1981 ttttttggtg gagctactgg gatttttttg taatagaaaa taatagttgg tatcgtctca
     2041 ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt cgtatccttc gaatgtcttt
     2101 tgctctgtca tataattttc tccataagct ccgtaaagct ggatttcgtc ctggtctttg
     2161 agtagacttt tattgttttc atccacatac tttacgttta tctgatcatg gaacactact
     2221 ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt caacttcctg tgcctttcca
     2281 tgccctgctc cttctactaa taaaagatct ttataccctc ctgataactc atataatttt
     2341 tgcgcgttag acacaggtac tacatcatct gctgttccat ggataatcaa tttaggcaat
     2401 gaatctgaca ttccaatcgt aggtagagca gcttttgtat ccaaatgcat ctcctgatca
     2461 aaatagtatt gtttcaaaag gcccataaat aaatcttcat gctcttgatc cccaatgatg
     2521 tctaactgat tttttatggc atccggtagt gcgggggcta ctgtctggct gtacgtaaaa
     2581 cgtagttgtt catcaattga tgcaaatccg caattttcaa taattccttt gacattgaca
     2641 ggaatatctt tcgctaatac agacatcatc gtagctgctc ccatcgaacc accataaagt
     2701 aaaatctttt gagatggccg ctctcgaact tcttgattta tccagtaaag gacgtcatct
     2761 gactcatatt gtccataggt cacataatcg cctccgctat taccagtcgc tctggaatca
     2821 acaaataata aattataccc ggccttataa aaatcattgt attcttctgt aacgatcccg
     2881 ttattccaat tccctcgaaa tccgccatgg atcatgactg ttttatctga accttgatcg
     2941 acataatatg catatacatc catcattttg ccatcttgtt tgtttagtac agtttgatga
     3001 agttgttttg tatttggata gaagcctgtg ttttctgtag gcgttgtccc atcatctgta
     3061 cctccaagca tcgacatcaa cacactgttt tttgcaaaag cgatatcgta caatagataa
     3121 gtagcaatca gatgtttgat aatctcttta tctacaccat aatcattaac agcaagctca
     3181 gtgactaata actctattaa ttttgacttg atgtcttctg ttggtccatt aagtatttct
     3241 gtcatctgtt taccgaagta attcaaatat tctttaccat tgaaattttc acctttaccg
     3301 atatcatcat tttctgtccc attctctttg tcacttactt tagcagaaga ggattcgtcg
     3361 tttgacattt ttgtagctgt tgcttcttct gtttgttggt tatctgtggt agcggtcgtt
     3421 tcttgatctg tgctagtaac agatgtctca accgttgttt cagtatttgt ttctgtttgt
     3481 gaagtactcg atttcgtttc atttccagca tcagtagttt ctgtggtttc tacctcccga
     3541 gattccgatt cggaagaaat gctctctcca tatactgtcc attgcgggac gcttgtactt
     3601 acagcaaata agatgaccaa cgctcccttt aatacttgtt ttgttctcaa gctattcact
     3661 cctttttatg tttctaggag aattatatgt aaacgcaaac attttttata caaaaaataa
     3721 atggtttatc ccataaaaat agaacaggat tattttctca ttttacgtaa taaagcagaa
     3781 tggataaacc agtatccatt ctgctttttt taaacgaaga aacaacttat tgctccaaaa
     3841 atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc gattgtttcc tttgctttca
     3901 taaaaggatt atcataagtt gtcgttgctc ctctacacat cataagctgg tatcctaatc
     3961 catgagcaaa cttcacagtc gtatctatac aatactctgc ttgtgcccca cataattcga
     4021 tcgaatgaca gtccaattca gttaataaaa cactcaaatt agtcttataa aatgaattcg
     4081 catgggtttt tctcacaaaa taactgtgct caggaataga taattccgac aatagctccc
     4141 atggttttgt ttgtggcact aattgagcat cttcgtgttg aacgaaaata actggtttcc
     4201 caatcgtttc gtatgttttg attcttttat tcactaatgc tgtcactgtt tctaattcgt
     4261 gtatcggcga agaatcaaaa catacccctt tttgtaaatc gatcactact attgcgtcgg
     4321 ctgtgtttga catacttgtg ccttctttct tttttcttga tttaatagta tttcttatct
     4381 agctagttcc attatacgtg acaacatttt cttattttac acctctaaat ggcacttttc
     4441 gactatttct tcatgttatt atttcataaa tacttagcta ttggaggaat gtatgatgat
     4501 taaattgtat acaggttctg gctgctcgtc ttcaagacgc gcaaaacgtt ggcttatcgt
     4561 ccatgatata ccgtttatcg aacagaactt aattcgtcaa ccactaacca aaaatgaatt
     4621 tatccactta ctaaaattga caaacaacgg cgtgaccgaa atgctctcta ctcattctat
     4681 tagttataaa aatcttgttc acagaataga tgaactcagt ttgagtgaac tatatattga
     4741 gattcgtaca tccccttctt tattgaaaaa accgattatc tacgaccaat ctgcaaaact
     4801 tttgactgga tttagcgaag ataaaatcag gatgttcctt tctaaaacta aaagaaagca
     4861 agagctcaac gaactcttgc tttctggtca tcttgtcgat tttaaagaaa ataatctttt
     4921 tgcaatgtct taattttggc ataaacaatc tgcttttctt aattaacgaa atccaatttt
     4981 tccaatatgg atctattttt ctcattcctg atttttatga tttcagcttt cagacgcgcc
     5041 caatcatttt ttgtaggttg tccattccag taaaatatct gtgtattcgt ttgatcatat
     5101 gcctttttgt cgataggata atcgctaatg acaatatcag gtaactgatc agcggatgca
     5161 ctatatttta ttgggcgttc agtcatagat aatatccttt tctttgccca taattcctgt
     5221 gttttaccat actttgattg aatatagaat atgacaggtt gttcgtgttt tataaaaata
     5281 tccctgacta acatacagta ttgaaaaatc aaccgtctgt tcttttcata ataagtatta
     5341 agctctggaa ttctgcgagc taccccacta aaaaactgtt gcatgattgg atacatcact
     5401 ggaaatatat gctgaaaatc tctgtcttct gctgattttc caaaagcatc caccttcttt
     5461 tttgtcccaa aaacccttga atacaaatgg atcattgcca aattgacata tagataattt
     5521 ttttctgaag ttgtaaaaca aatcttgaag tttttttcaa aattttctac aatagacgat
     5581 tcaatccagc cccaactttc ttgtacttta tctggagtat ccaacaaatc ttcttccaaa
     5641 taggtgttca ttacaccaaa cataaaataa aggtaagctt gctctgttgg agtgatgtga
     5701 ttcatcgacc agaagtttgc ttcaaaaaaa ggctctagta tgtaatgcgt aaactcttct
     5761 tttgttatca atggatgcac aaatttgttc atctcttctg ggacatacgt aatcgagtgg
     5821 ccttgtttca ttcgttgcaa cgaaattctt agtaggctga tccatttctt tctatctata
     5881 ctacgcgtat atggcgcatg tttcgcaaat ttttctaata gttgacgttt attttttata
     5941 cttgataata tatcagaatg attttcgtcg aatgactgcc aatacatcaa gtgaaaaaag
     6001 tatcggatac ggtattcttc tccttctatc ggattacctt ttttcaagct gcaagataaa
     6061 tgatagtttt tcaaaatagt attaagttct cttaattttc tttgaatcgt acgcaaactt
     6121 atataatttt ttgactaaga ttttctaagg attgataagt acctttgtaa agtgaatcca
     6181 gtaaaatata accaatcgtt ccctctaaaa gtatagaata aactttactc agtaaaaact
     6241 gagaagaaaa ctcgactttt atcatcttgt tttctgaaag aaaagataat ttatattctg
     6301 gatacctttc gcaaaacgat tctgcttgtt gaatcaccga attcatctta gccagggtgt
     6361 agtttagatc taaagcgata tccttcacca catataattt ctcgtctctt tcataacaat
     6421 agttcaaaaa ttgatatact tgcctttcat tgatatcaat aaattcttca aacaaagtac
     6481 atactccttt ttatctatta tttagactta ttccgacagc tacatgaata acgtacaaat
     6541 agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt caattattct
     6601 tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca
     6661 aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg aatgaaatat
     6721 tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt ctttatttca
     6781 ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt aacacaccca
     6841 tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg ctttttctat
     6901 tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa tcaggctgtt
     6961 ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt ggtatgatag
     7021 gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg
     7081 tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc tccgtactgc
     7141 tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     7201 tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc tgtactgctt tcaccgctcg
     7261 tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt ccaacaactg
     7321 tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa aaaacaatca
     7381 aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa atcattattg
     7441 tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca ataacttttt
     7501 ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca ctagcattca
     7561 cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc
     7621 gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca gaagtactga
     7681 ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg gtcgctgttt
     7741 tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa ttatataaaa
     7801 tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc
     7861 gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc aagaccgata
     7921 tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc atccgctttg
     7981 ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat gtagttgata
     8041 tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat tggatcagag
     8101 tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata gctccttgtg
     8161 tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta ctgtagataa
     8221 gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat gtcatgttag
     8281 gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct attaacggac
     8341 caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct gatgccccca
     8401 ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt cctgaatcta
     8461 accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact ccaattccaa
     8521 caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca ccataatctt
     8581 ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa acagctaaat
     8641 gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac atatccgcaa
     8701 gtgcattatt gacaagaatt cctaaaacaa aaccaactaa gactattaaa acagatccaa
     8761 tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta acaacccttt
     8821 tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa tagattttta
     8881 cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc taattcttct
     8941 gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta
     9001 tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc aatgaaacga
     9061 ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac ccaaaagtta
     9121 aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat
     9181 acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa atcaaaaaac
     9241 tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag acatataaga
     9301 aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta aaattaacag
     9361 ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca cagatcattc
     9421 tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa ggagaaaatt
     9481 tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa
     9541 tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca gcatttaaac
     9601 taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact tatcagcaaa
     9661 gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa gacaaataca
     9721 aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa ttcatggata
     9781 aaacaatccg taaagtgatg aaaaaagaca atgtaactat ta
//
LOCUS       DAFCLG010000097         9786 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFCLG010000097 DAFCLG010000000
VERSION     DAFCLG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.097"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(71..277)
                     /locus_tag="LND56_002158"
     CDS             complement(71..277)
                     /locus_tag="LND56_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744037.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTIRGLFNYFNK"
     gene            complement(293..907)
                     /locus_tag="LND56_002159"
     CDS             complement(293..907)
                     /locus_tag="LND56_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK6744038.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1054..1737)
                     /locus_tag="LND56_002160"
     CDS             complement(1054..1737)
                     /locus_tag="LND56_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK6744039.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1846..2304)
                     /locus_tag="LND56_002161"
     CDS             complement(1846..2304)
                     /locus_tag="LND56_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK6744040.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2388..4151)
                     /locus_tag="LND56_002162"
     CDS             complement(2388..4151)
                     /locus_tag="LND56_002162"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK6744041.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4372..5832)
                     /locus_tag="LND56_002163"
     CDS             complement(4372..5832)
                     /locus_tag="LND56_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744042.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6040..6966)
                     /gene="glsA"
                     /locus_tag="LND56_002164"
     CDS             complement(6040..6966)
                     /gene="glsA"
                     /locus_tag="LND56_002164"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK6744043.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6989..8416)
                     /locus_tag="LND56_002165"
     CDS             complement(6989..8416)
                     /locus_tag="LND56_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6744044.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8447..9661)
                     /locus_tag="LND56_002166"
     CDS             complement(8447..9661)
                     /locus_tag="LND56_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK6744045.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2894 a         2032 c         1625 g         3235 t
ORIGIN      
        1 caggattctt tgataatttt ttagttttat gtgttttttt atatgttttt tcacttttcg
       61 gaataaatat ttatttgtta aagtaattaa acaatcccct aatcgtttga tctggcattt
      121 ctatttcctt accatctaaa gtaacagtag taacgacaaa atggtcttgc aatggcggaa
      181 cttctaagta ctgcccttgc agaaaatcat tcactgtttc atattctttt ttcatttctt
      241 ttgaatctgt cgatggttta tagcttattt ttaacataac tagtgctcct ttttattcgg
      301 gacgtgcaat cgtgaaaatt tctcctattg ctgcataatc atttcctgcc aaacggctta
      361 cagcattcaa ttttcttgga tcgatccttc ctgtcttatc atcatagact gtttcattta
      421 aatgatattt ctgtacttct agcaaaaata aatcagctgt tactttattt ttattccgta
      481 tctcgacatg ttcatataag gatgtttcaa aacgaacttt tgcttctgtc aatgctggta
      541 ctgaaatgat tactgaatca gttaaagtca agtcagtcag gtacagttcg ctttcctcat
      601 taggcagatt ggctgctgtt ttatttactt cttctacgtt gtcctcatca aggatatgga
      661 taacagcttc tcttgtctca agaatattct ttgctgtgtc ttttactgca tcttgttttc
      721 tctggatcgc caaggataag atgggtcgat tcgaactgac gatattgaag taactgaagg
      781 gtgcgatatt cactatatct tttgctgatt tagtcgttac tactgctact ggtctaggga
      841 tgactgaacc aatcagcaac ttgtaatttt cacgttctgt caattctgaa ggactccgtt
      901 caaacatggt ttcgccttct ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa
      961 tgaaaagtaa tgcttttttc atcttcttag aatctgcttt aaactatcta tcaatttttc
     1021 ttttttgtca agaatctgtg ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc
     1081 gacatatctt ttagtgatca attgcggacg ggtgataata gatccaacta cgactgaaaa
     1141 agcacctaat tcatatgctt tctctacttg ttcaggagag tcagtatgtc cttccataat
     1201 tactgggata tcgacttgtt ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt
     1261 ctttcgtgaa tattctgtat agcctgctaa agtcgtagac aaaagatcaa aaccatcttc
     1321 ttgcgcttga agcgcttcct caaaagtcga aatatctgcc atagctaacc gaccgtgttc
     1381 gtgaatcatt tggacaagtt cagcagttgt tttttctgtc ggacgtttac gagaagttgc
     1441 atccagtgca atgatttctg cttgcgtctc caaaagttct tccacttctt tttctgtcgc
     1501 agtaataaac acttctgacc catcgtaatc tcttttgata atccctatta ccggtaaatc
     1561 aacgattcgc ttgatttcat tgatatcttc tgtcgtattt gctcgaatac caactgctcc
     1621 gccttctttt gcagccaaag ccattctccc cataataaaa gaactgtgta gcggctcgtt
     1681 ttccaaagct tgacaagaaa cgacgactcc gcctttgatt tgttccagtg tatccacaat
     1741 atctcctcca gtaatgttat ataaaaatac agcttcatat aaaaatgtaa cccttttcta
     1801 ttcaggagac aataatgagc tatttctttc agcagacctt tcttactaaa agaaaattac
     1861 ttcaaattct tcgtatacga ccagcttctc ttctgtaaat tctatgttca acgcttcttt
     1921 ataagcatgt ttaaaaaact cagcatggac tttccgccaa taagctaatg tccgatcccc
     1981 ttctccttct ttccaagcat gctctttcgt gacttcacta aaaggcaaga tggtcacatt
     2041 tatatttttg atgatacaaa ctggttcatt ttttccattc aggaccacgt ctaattgacc
     2101 aacttttggt aaaggctcat tctcttgttt atacagatcg taaccacttg atgtaccagt
     2161 ctttatcttt tttacaacta gttcgctcaa ctcgtctgcc attttttctg tatccccaaa
     2221 tgaaaagaaa cctaattttt ctgattgata actaggatat ttttcttgga actttttgac
     2281 atattcttct acttgctgat acataacatc cacccctttg ctttttataa tgaaaaaacc
     2341 atccagatac tagtataaac aattatctgg atggtgagca taatgaatta ttgaatattc
     2401 aagacacgca gactatcttc aacgatttgt tttggtgtta aatgataacg ttcataaagc
     2461 tgccctacag gaacgctatc cgtgaattct ttctctgcac caaaatttaa tacgttcatt
     2521 gaagaatcac cataataacg actgatattt tcgccaaatc ctcctgacaa actaccatct
     2581 tctaatgtga tgactaattc gtgtgtttct tttaatttat ctaatgtttc tacgtcattt
     2641 tctgtgatcg ttcctggatt gatcaacgtt gcttcgattc ctttttttct aaaagctttt
     2701 actgtttctt ttcctaattc aagaaaaccg cctaaagcta aaattgctac ttttcctcca
     2761 ttatttactg tttgataggc tggaatacta taatcatttg cgatagctgt accatgttct
     2821 acaccatgtg taggaattcg gattgctaca ggctgcgctg tttgttcgaa cgcccaatct
     2881 agcatagaga tcatatcctc ttttgttgct ggagcaagat atttgatgtt cgggatactt
     2941 gataagtaag gaatgtcaaa tgttccttgg tgtgttcggt cactagctga gatcgttccg
     3001 ccaccgacta aaataatcac tggattctcg ttgatcgcta aatcatgtga taattgatca
     3061 tatgcccgtt gcaagaaagt acttgattgg aagacgactg gacgtactcc ctgagaagcc
     3121 aaagcagaag cgaaagtgat agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct
     3181 ggatgttttt cttggaatgt tttcagtcca aaggcgcctg gtatcgctgc attgactgcg
     3241 gcaataggag ctgtacttgt ttgcattttc ttatccaaat aatccatgat gatcatatta
     3301 aatgtttctc cagaagttac ttgctttgcc tccccggtgg acagatcaaa tggtacatgc
     3361 caatgatatt tcattttatt atcgattgcc ggctgataac cgcgaccttt ttccgtatgg
     3421 acgtgaagta caatcggatg atcaatatct tttacttctt caaataaacg gatcaccgtt
     3481 tccagatcgt ttccattatc cacgtaacga taatcaaatc ccattgcttt aaataggtta
     3541 ttgctgcttt taccgttggt ttgacgaagc tcagctaagt ttttatataa actgccatga
     3601 ttctcatcaa tagacatctc attatcgttt actaatacga ttagattgga ttgtaaaaca
     3661 ccaccattgt tcaatccttc aaaagctaag ccaccactta gggagccgtc accgataaat
     3721 gctaccacat ttcctgattc tttcttcaga tctctcgctt tagctaaacc tgttgctaag
     3781 ctgatagatg tagatgtgtg ccccactgta aaatagtcat gtttactttc atgttggttg
     3841 gaatatccgc tcacttcacg gtaatgtcct ttttcaaaac cattttttcg accagttaaa
     3901 agcttatgac cataagactg atgggaaaca tcccaaacga ttttatcctt tggtgaatca
     3961 aatacataat gaaacgcaat cgttaattct actacaccta gattcggacc aacgtgccca
     4021 cctacatcgc tgtccttttg caagattttt tggcgcatct cttctgccaa ctgtttcatt
     4081 tcctcaacag ataatttttt gacatctgcg gggctattga ttgcatccaa tacatgtaat
     4141 actgtactca taatacattc tctcctccgt actatcttct tttaatgaaa atagatttgt
     4201 tacactttat ctattctact acttagagtt gactctaagt caatagcatt ttaaaaaaat
     4261 ttcagagaaa gcttgttata taagtagatc ttcgcacgaa tcaggactca aataaaaacc
     4321 ttctacagac aattaaaaag ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat
     4381 atgttttttt aataggtgaa gttcctgagc aatatgatac aaatggatgt ttgcaccttt
     4441 taatgagatt ggatagatct ttgtcttttc agtttctaat ccattttgat agctagcagc
     4501 atcgcacaaa accactcttt ttttcgcaca tagacattca tcaggcaatt cttctaatga
     4561 acgaaataaa ataggattta tttcagcgat gccttcttcc gcatacaaat gaaattgggt
     4621 tcgataaaaa aattgtttga ctgaactttc tgaaacaaca aaaatttcta tttcctgtct
     4681 ccccgcttgt tcactgttga tcaaagaaag acttttgacg aattttcgaa tgaaattttc
     4741 attccatctt agttggattt gatttttctt tttccactct tccaatattt ctacaatttt
     4801 tccgtacaga agttgttctt tttctgatag aagatagatc ctttccggta aaaatacttg
     4861 tccatctgcc cacaacgtac ggacaaattg taaaaaggat tgcttgaaaa taaaattttc
     4921 tgcatcgtcg atttcagcga tactaataat ctgtaaaatc aattcattca gtgataggtc
     4981 attgagaaca aaatttttat aaacaacttc gaaatctttt cttaacagct ccatgttctc
     5041 attggtataa tttctactgt tgaacaaaga aaaaatataa tagacatctt cttcttgtaa
     5101 gtctagaatt cctttttgct cttccaatcc ttgacagact aattgaaata acggcttcgt
     5161 ttctgcgctt ttcttatctt cttctttcag tccaagggga aatgcctttc ttccgattgc
     5221 gatctctatt cctcgtatga taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa
     5281 atgagtctct acataatcaa tcaactcgtt acaagcttct tctacatacg gatttttatt
     5341 ttcatagcct tcccaattga cataacgtac taaagctagc aagatgttcc gcgagtcaaa
     5401 ttctgggata atgatttctt tatttttcaa ataatcattt tctttaaaaa aatccaatac
     5461 gatttttttg attgtatata ctttgctcaa agaaataaat tcaagatcag ccagttctgt
     5521 actcgtgaat ataccattaa aataataact taaaaacttt aaaaaataag attgaccgta
     5581 cagaaactgt gtgagctcag aaagagcaat accatgtttg aatacgagtt tatacttatg
     5641 cttctctctt acttgttctt ttatacaata ttctagacac tcggctagac tttctagatc
     5701 gtgaaggatc gttggtgtag tcacccttag agaaatagct gtttgttcga tgtctatttc
     5761 ttggaattca aataattgtt cacacagata cacttttcta aatatatttc gctcgatgta
     5821 attttccagc attcctgata cacgcctcca gtttttctta tgattttatt taatcatatc
     5881 tgtaaatcgt atttttgttt cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa
     5941 aagaaactgg gacaaaagtc aaacaagctc acaatttcag cctcattgta ggtagctttg
     6001 agccgtttgg acttttaccc cagcctcttt caagaatggt tattcaaaga tgtcagcgtc
     6061 tagttctttg actacatctt tcaacaattg gataccagct gcgctgttac caaatggatc
     6121 tagtgccggg ctgaagacac ctatgccgta acgatttggc acagctgcca tcaacccgcc
     6181 gcctacaccg ctttttgcag gtacaccaac atggacggaa aactctcctg attcatcata
     6241 caaaccagca gttgtcataa ttgatttgac gattgtagca ctttcttcag taatgatttg
     6301 tttgccattc caaggagcaa ttcctttatt tgccagcaca gcagctaatt ttgctatgcc
     6361 tgtagcggtg acattgaggg agcattgtct gaaataagca tcgagtatct ccggtacgtc
     6421 tccttcgatc atttgattac ctttcatata gtaagcaaga gagcgattga tatcacctgt
     6481 tcttgattca gaatgataga tttcttcatt caatgtgata ttaggatcat cacagatctc
     6541 tttcataaat gacaagattc gctcaaattt ttcatctgca tctttaccgg caatcaacga
     6601 ggttgttgca atcgctcctg cattaacaaa aggattcatt ggatgtttgc gatgattgat
     6661 ttccatattc atgatagaat tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa
     6721 gactttttct attccgttgt ctttgattgc taataacaat acgggaactt ttgacatgct
     6781 ttcgatggca aagcgcacat cggcgtctcc tccctcaatc agttgttttg tatccaaatc
     6841 ataaatcgcc atacctaatt gtttgggatc cacttcagct aatgctggaa tgtaatccgc
     6901 cacttgtcct gtttgataat agtggaaatt ttttccaatc aattgttcta attcttttac
     6961 ttccatttga ataaccagct ttcatttttt atttttctgc atgctggaat ttatgacggc
     7021 atgcgtagat cgtaaatgga ataactaaga cgataagata agaaacaatc agaatcgttt
     7081 cataaatccc atgctgtcca gctgcgatac tagcaggtgg aacaaatgag atacaaaaag
     7141 caaaaagtga agtcaaaaga cctaaacaag caacgattac ttttacaact tttccgcctg
     7201 gaacctgata ggctctttca agactgtcat ctttaaaaac cagcttcaaa tagcccgcga
     7261 aaaacataaa gtacaacatc aggtagatta caacagtcaa tgacatcgct gcaagaaatg
     7321 aaagattatt ccccccgccg cttaatgtaa agacggctgc ccagattgtt gcaacgatcc
     7381 catttgcgat catcaaatga gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag
     7441 gaagtaatcc ttcttctgct gctacgtgaa gtgcttttga aggagaggtt acccaagaag
     7501 caatttctcc gatgacacct aaacaaacaa gaatcgcaag aattttcgca atccatgcaa
     7561 gatttggatc aatgtgtctt aataggaatt ctaacgattg aatcacccct gtattaagag
     7621 acaaaccatg ttgaggtact gtagtagcga cggtcaatcc gccaaaagta tctaaaatga
     7681 ttgccagaat aaccaataca aacattgcta atggataatc ctttttaggg ttttccattt
     7741 catttgcatg actggcagat gtttctacgc ccatatatgc taaaataaag gaaacaaaaa
     7801 caaccagcgt aggcaatttc gaaaaatcag gaatcaatgt tttcatagac agtatcgttt
     7861 gtagtggagc gcctgaagcg acataatgga tccctaagcc aaacaagact aatgctggca
     7921 acaagatacc agctacaaaa ccgatccgag caatcaatga agtgtatttg gttcctttta
     7981 actgtgagag taaaatagcc cagaaaataa tcaataggat tgccaatttc agatatttat
     8041 tgttttgtat agcaccaata cccgttgcat aggataatgc accagttaag aaatacagca
     8101 tggtcaagta gccaactgtt atctcaaacc attggaaaaa gattgcggca aaaccgaatc
     8161 gttttcccaa tgtgcgactg acccaagtat agacaccgcc tttttcccat cctttcaccg
     8221 ttgccatctc tgctgcgcac aatgccacag ggataaacca tagcaaaccg cctaataaca
     8281 agaaaaatac taagctgaag cctgaggtcg cgaatgtggg atattcatat aatgaaacga
     8341 ccattgagat ggtcatagcc aaaaagccaa agaacgttaa tttttttgca ggaattttcg
     8401 ttttgctttg ttgcatgaat cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt
     8461 tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc ttttgtagta gtgatcgttg
     8521 taaagtgcat cgccgatttt aaaatcactg ttgtcagcac ataggcatac acggcagtaa
     8581 tgatgacacc aatcacttga atccctaaga agtaccagct gcttggattc gttagtaaat
     8641 ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt gaatccgccc atgccatgaa
     8701 caccccacac atcaagtgca tcatcccatt gtttcttttt acgaaaatca acagcagcat
     8761 gacagacgat acccgcaatg atcccaatca gcatggcact ttttgcttct acataaccgg
     8821 cacaaggcgt aatggctgcc aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa
     8881 agccatttcc ttttactttt gcaatgatca gccaagtaat catcgcaaat gcaagagcga
     8941 tgaatgttga accaaatgca ttgactgctt gttcatctgc tcgaagtgca ccaccggaat
     9001 taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat agcagctgcc ataagactgc
     9061 tatgctgact tctttctcct aaaggaatct ttctttgacc aagtgtgagg atacatgcta
     9121 aagagccgaa tccagctgtc gtgtggatga ctgctccacc agcgaaatct ctaaatccca
     9181 tctgatccaa aaatccgcca ccccatatcc agtggcaaac agggaaataa atcagcagat
     9241 tccaaaaaat aacaagtata atatagcctt ttaaattcaa tctccctgca aaagctcctg
     9301 tcatcaaagg aacggtaatc acgataaaca tcagttggta aagaaagaac attaaaaatg
     9361 gaatcgttgc accatgaaac atgttgggga agcttgtaac atgtctcatc aaaaagaaat
     9421 ctgccgggtt tccaataaca ccgccgatat cccgtccaaa ggccaatccg aatccaccaa
     9481 atacccacat aatcgttgtt atcccaacgg agagaaaaga ttgagacatg atcgttagta
     9541 tattattctt ccgtgctaat ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa
     9601 ccattgctgt acataaaatc atgaatgctg tatctcctgt attgataggt aatgtattca
     9661 tttttttcgc ctcctttcta cgattacgag tataaaagaa ggtgtagttg tttttttctt
     9721 ctgttttttt gaattaatac ttgcatttat aaaagacaga caattaataa aagcttgatg
     9781 tgttgt
//
LOCUS       DAFCLG010000098         9512 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFCLG010000098 DAFCLG010000000
VERSION     DAFCLG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.098"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..2019
                     /locus_tag="LND56_002167"
     CDS             91..2019
                     /locus_tag="LND56_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6744046.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2157..2612
                     /locus_tag="LND56_002168"
     CDS             2157..2612
                     /locus_tag="LND56_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK6744047.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3082..3351
                     /locus_tag="LND56_002169"
     CDS             3082..3351
                     /locus_tag="LND56_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744048.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3751..4005
                     /locus_tag="LND56_002170"
     CDS             3751..4005
                     /locus_tag="LND56_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK6744049.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4094..5140)
                     /gene="holA"
                     /locus_tag="LND56_002171"
     CDS             complement(4094..5140)
                     /gene="holA"
                     /locus_tag="LND56_002171"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK6744050.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5178..7451)
                     /locus_tag="LND56_002172"
     CDS             complement(5178..7451)
                     /locus_tag="LND56_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBK6744051.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7444..7983)
                     /locus_tag="LND56_002173"
     CDS             complement(7444..7983)
                     /locus_tag="LND56_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBK6744052.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8119..8337)
                     /locus_tag="LND56_002174"
     CDS             complement(8119..8337)
                     /locus_tag="LND56_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK6744053.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8334..8579)
                     /locus_tag="LND56_002175"
     CDS             complement(8334..8579)
                     /locus_tag="LND56_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6744054.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8695..9423)
                     /locus_tag="LND56_002176"
     CDS             complement(8695..9423)
                     /locus_tag="LND56_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK6744055.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2933 a         1853 c         1658 g         3068 t
ORIGIN      
        1 gataaccatt atcgttctca tttagctgtt attattatca attacatttt tattatgcta
       61 tccttgtttt gacaaggagg tctaataacg atgactaatc agaaaaaagc ttttgcagca
      121 acaatcggct gccttttatt tatgatcttc ggttttatct tcgaccgatt ctctcttacc
      181 atcaccccac ttttttatat cagtgccatt atcatcggtg gatataaaca aacatcggaa
      241 ggcttgaaag aattgtggaa tgatcggaca ctgaatgtgg atttattgat ggctttagct
      301 gctattggtg cctgtttgat cggtaattac tttgaaggag caatgttgac attcatcttt
      361 tgtttaagcg gtgcattaga ggaatacaca actaataaaa gtcaaaaaga aattactgct
      421 ttgatgaatc ttcaaccaca aaaagcccaa ttattaaaag aaaatggaca aatgcaagaa
      481 gtagatgttt ctcaactaaa ggtggataac ttggtatttg tcgctaaagg agcagctgtt
      541 ccgatagatg gctttttaga aagcacacag gcaacgatcg atgagtctgc attaagcggt
      601 gaatctgtgc ctgtagaaaa atatttaggc gatgagatgt atgcaggaac gctgaatcaa
      661 ggaaatccca tcacgattcg agtaacaaaa acgagttccg aaactgtctt tgctaagatt
      721 atccaattag tagaagaagc gcagaatacg ccaactcaaa cagcttcatt tatcgaacga
      781 atcgaaaata attatgtaaa attgattttg cttgctgttc ctctaatgat ccttctcccc
      841 cactttttcc taggttggag ttgggatgaa agcttttatc gaggaatggt attattagtt
      901 gtggcttctc cttgtgcgct tgtcgcatct gctacaccag cttctctagc tgctctttca
      961 aattctgcaa aaaatgggat tttaatcaaa ggcggtatcc atttagaaaa gctttctgaa
     1021 ttaaaagcga ttgcctttga taaaacagga acgctcacta aaggaaaacc agttgtaact
     1081 gacagctttt tctttgatga aaaggatttg gcacaacaac ttcttgttgc tatggaacaa
     1141 aaaacgacgc atccacttgc tcatgcgatt ttgcaatatt tcgattgcac aatacctgaa
     1201 gaaatccaat acttaccaat agaagaaatt actggttttg gattacaaac tacctattta
     1261 ggtgcgcaat ggaaagttgg caagcacgca tatgatcctg agacgatgat aatcagcaaa
     1321 gagattgcgg aaatgatcga acgtttggaa aatcaaggca aaaccgtgat ttatctttct
     1381 aaagatcagc agttaattgc tgttttaggt ctgctagata ttccaaaagc aaatacccaa
     1441 caagtaattt cctactttaa atcccaaaac atccatacaa gtatgataac cggcgaccat
     1501 tctggcacag caaaggccat tgctgaacaa gtagggattg aacattacta tgcaagctgt
     1561 actccggaag aaaaaacaca actagtcaaa aaagagaatg aactttacca agtaaatgcg
     1621 atggttggtg atggagtcaa tgatgcgcct gctttagcag ccgcttcttt aggtgtcgct
     1681 atggggcaag ggacagatgc cgctatggat atcgcagaca tcgtcttaat gaaaagcgac
     1741 ttaaataaat ttgtctacag tcatcggcta tctttaaaaa tgcggcggat catcaaacaa
     1801 aatattattt tttcgattct tgttattctc ttgttgattg cttctaactt cctgcaattt
     1861 ttaaatctac ctcttggagt tgtaggacac gaaggaagca caatattggt tattctgaat
     1921 ggcttgcgtt tactaagacc tttacctgat ctttctgatt cttcaaaaaa atgtaagtct
     1981 tgtccactat acaaagcgcg gctgtcttct gtcaaataag caaagtgttt ttttatgatt
     2041 ttgtttattt tatgaaaaaa acagcttatg aataggtctt gaaggatgaa ttcctacttt
     2101 tgtaactttc taaaaacatg ttatgattgt ttcgaagaaa gtgaggaata tatttcatgg
     2161 aaagttggct ttttttagga attattttac ttgtagcaat tttagctaaa aatcaaagtc
     2221 tgatgatagc ggcagctttt gttttgatta tgaagctcat accagcctcc cgttcctttt
     2281 tcccttggct tcaaggcaaa ggaatcaata ttggggtgac gatcatcaca gcggctattc
     2341 taatccctat cgcaacagga gaaatcggtc tacgtgatct attagaagcc tttaaatccc
     2401 ctattggctg ggtagctgtt ttttgcggtg ggctagtggc tgtcttatct tccaagggtg
     2461 taggattgat tgcttctgat ccacaaatca ctgttgcact tgttttcggc acgattttag
     2521 gtgttgtctt cttaagagga atcgccgccg gaccggtcat tgcttctggt atcacttact
     2581 gtatcttaca agtatttgct ttaggaaaat agaacaaatt ttgtctattt taggagattc
     2641 ttaacacccc caatttactg atccgtgata tactgacaac gaggtgatac aatgttcgaa
     2701 aaattaaagt atttcatagg aacaatcggc tggtttcttc tagcggtact tttgattctg
     2761 ggcttagctt atctattatt tcctgatttg atacaaaatg tgtttgatac tgctttttga
     2821 ttgacctatt aaaacgatat aggggatgct cctgcgatgc aggggctttt tttaatcaat
     2881 gttttgtaat aatttaggcc gtgccaatga gacttcctat agaattcgat gctctttttt
     2941 ctttttggct cttctgctct tttttgttat actagattac agtggacaaa agaaagaatg
     3001 atcgcatcca cttcaacgaa tatcaaaatg gaagaattag tgcctgtttt caggttactt
     3061 tgaaaagaaa ggaagacgct tgtggaaact tttgaaggtc ttttaattga tacgtacgtt
     3121 ttacaagaac agccaatgat gctgactggg atcaacttgc agcttgagga ccgtgtgatc
     3181 aatggcctgc ttaaagatcg ctatttggca aaatatatct tacgtttgcc cctaaataaa
     3241 tatgtgctgg aagtaagtgg caaatggaac cgtcggaacc agttggtcat aaaaaaaatg
     3301 ttgataaaaa atatcgatga atacattaaa ataatcggta caccagaata gcctttactg
     3361 atcaaaggat actggttata aaataactgc agtttcttaa tagaaaaaca aagaacaaca
     3421 gggaatcgta tcccttttca aaaaaaactt tgcaaacggt tctctttgtt atataataac
     3481 ttagtaccaa acattcccat tcaaactttg tttaaataga taaataaaaa tctagtttgg
     3541 tttgacgcta ttgcctccca caatagcgtc tttttgattt ttttcagctt gtactatccg
     3601 tttttactga aaaaaaatcc taataaaaga ctgttaagga aaaaaacttg tcatgttcat
     3661 attgcaaagc acgctgcctt taggtataat aatgaatgtt gaattatgcg gtaacacgcg
     3721 taagaactta agttatcgga ggtgaaacta atgccaaaca ttgaatctgc aatcaaacgt
     3781 gttcgtacaa gcgaaaacgc taatgtgaaa aattcatctc aaacaagcgc tatgcgtact
     3841 gcaatcaaaa aatttgaaga tgctgtagct tcaggtgctg acaacgtaga tgcgttatat
     3901 aaagaagctg ttaaagctat tgatatggct gaatcaaaag gtctaatcca caaaaacaaa
     3961 gctaaccgcg acaaatctcg tttaagcaaa aaaatcgcta aataaaagaa tcggcttcgg
     4021 ccgatttttt tatttgtcaa aaaaaggaca aaaacaaacg accttcccgc atgctttgcc
     4081 ctcgtctttc ttcttatcga tttcttaaca actgccctgt gcgcaaaacg aatagttgaa
     4141 aacttaattc tttttcgatt tgtcccgttt tgatttgata atccatctca actaattgat
     4201 caaacaactg taccagcaaa tgttcatcga atttttttac ttcctgcatg gcaagtttta
     4261 cacgatatgg atggattttc aatgtttcag caatatttgc ctgctgataa ccaatcttta
     4321 caagaaattt tgtctgtaaa aataaacgta attgagaaag caaaatcgca ttaattttga
     4381 ttgtttcttc cccttgcata actaaatctt cgtataaacg caaagcttgt tccgtcttcc
     4441 cttttaaaat atattgggtc atgtcaaaaa tattatgttc taacgtttta ggaactaatt
     4501 cttctacttc tcttaccgtg atttgctgtt gattttgtcc ataaaggacc aatttctgta
     4561 actctctcat cattttggat aaatctaagt cggtcaacct caagaataaa tcaattgcac
     4621 ttcgttccat tttgattgag gaattctcta aggtattcat cagatattga cgtacttctt
     4681 tttcattcaa ctgttggaca ttgatgataa ttgctgtttt tttcaattgt ttcgttactt
     4741 ttttccgttc atccaacttt tcatatggcg caaagaatac taatacagtc gattctaatg
     4801 gttctttcaa ataagccgtc aataaatcag tattttgttc gattccattg tttacttttt
     4861 cacctgttaa aaaataagga ttctctacga atactaaccg ttgatctccg aaaaacggca
     4921 acgtctctgc ttcagctact acctcatcta aacttgcatt ttccatgtca aaacttaaaa
     4981 aatttaagtc atcttcgccc tctaattgga tttttttgac aatttctgtc cgaacttgat
     5041 cttggagaaa cttttctgtt cctaaaatca agtaacaaga tgcaagtttt tctttacgta
     5101 ttttttgtaa ctctgcttgt atactcaaat tactcaccac tttcaaaaat tatcctacca
     5161 tgagcagatg agaattacta gtctatcatt gtttccacta gcccatgagg atgaaaaact
     5221 ggatgccagc gataggtgat cattccttgt tgatccgttc gccatgtttt tatctgattt
     5281 tcttctagtg tctccaatac ttcgctgtgc gggtgaccat aacgattatt tacgccgcag
     5341 gatataatcc catgttttgc ttcaatatgg gaaagaaaat ctgtgttact tgacgtacgg
     5401 ctaccgtgat gacctaattt gactacatcg gctttcagat taggataaat caaaagcaat
     5461 tgtttttcca ttgcttgatt caaatctcct aaaaataaaa agcgcctatt gttatattcc
     5521 atccatagac caatcgaatc ttcatttctt ccctctccct tttcaaaagg agtcaataca
     5581 taaagatcat aataaccgct gatcctgtcc ccatgtttga tttcaatgat ctcagtacca
     5641 tttttttgag cttcagataa atgcctttgt aatggctcat ttcgtccagc gccccaaccg
     5701 atataaattt ttttgatagt aaaatgctga gataacgtgg gtaattctcc cacatgatcc
     5761 gtatcgtcgt gtgtcaagat gagtttatcg attacactta tcccctgacc ttttagatat
     5821 ggaacgatat ttctttcagc aggaggacgt atcatacctt gcgcccactt ttctctttca
     5881 aagtgtaact gacctccagt atcgatcaaa atcgtttctt ttcgaaaagg actttgcaaa
     5941 aagatactgt ctccttgacc gacatcgata aaagcgatcg ttgaagaaaa gcgtaaaaat
     6001 ggcaaagtca aaaaaataaa agaaaaaaga ccaacataaa acaagtgttt cacaggttgt
     6061 tctttcaacc tatccataca aatcagtaat ccgaaaatat acatcccaga tagcacaagg
     6121 ggcaaacgtc ctataatgag agacgaccct gaagaaaaat cgattattcg ctcaaataag
     6181 attaatccct cttctatcag caaaagtaaa gtttccggca gccagcgatt gaagagtgtg
     6241 agaaataaca caccaggaag aaaaagaaac ttgaataccg gtgcaatcag taatgtaaaa
     6301 agactaccta aaataggcca ctcataaaaa tgccatacaa tcaacggcat cgtgatcata
     6361 gtaaacagga cgtgagaggc cgtttttttc aaccaatctc catcaaaaga aatcaggata
     6421 aacaaaaaac tcatgaaaaa actaagctgt ccacccgcat gaaaaagaaa tcgaggatca
     6481 aatagtaaaa gaagccaaaa gacaactgca aataagtcca tggtggaata atgcttattc
     6541 agcaatttga ctagcaatcg caataaaaac agcaaactag cacgcaaaac agaaatagaa
     6601 cttcctgcaa tagaaacata acagatagtt agtagactca acggaataat actgacgtgt
     6661 agtggccatc cgatacgtcg aaataaataa tacagccacc ctaaataaaa ttggatgtgt
     6721 agacccgata gactgaacag atgcagcaaa ccactctccc taaaaatttc ttctggttct
     6781 ctgaatcctg catctttata gccccataat aaagaattaa tatatacact tgttttttgc
     6841 ggaaattcag attggacaaa ggagataaaa gatcctcttt ttctctgaag ccagtttcgc
     6901 cagtgatttt gcatggtgat ctgttcaatc ttttgaattt ggaaccgtcc agaaattcta
     6961 tggttcctga agtaagcttc ttggtcaaaa ttataaagat tccgcttttt tgctggagag
     7021 atgaactcgc cttcagcagt caattctatt ccctttcctc ggaagttaag ccatgttttc
     7081 aattctgctt ctgtgtttac ttgataagaa agctggagct tttgtttatt ctctgataac
     7141 cttccttcca ttgtcaaaaa attaccattt gcacgaatcg tatctgtaaa tacagtaatt
     7201 tgcgcagatg ttgttttttc ctctaaaaat gaatactctt gtagcctata agaaatggat
     7261 accgttatgc tacacaaaat acacaagtac gcgataaaac gtccttcttt tagcgaaaac
     7321 cataaaaaaa ttccggcaag aagcaaacga taaccccaat tgtcagtgta atacaacaaa
     7381 cagataatca tcgacgtaat tgccggaaaa atcaaccgat ttcgaacatg cctttctaaa
     7441 acgctagaca tgttttcctg ttaattcatt ttgaagtttt gaaaaatgct gtgagtctaa
     7501 agcaacttgg tgtacagata cacctaattg ttcgatcaac gcttgggcat aaggatcatt
     7561 tcgatagtca tggagataat tgatcccttt cacaccagct tgcaacaaag cctttgtaca
     7621 ggccaaacat ggaaaatgtg tgacatagat ttctgaacct tccgtcggga taccaagttt
     7681 tgcacactgt aggagtgcat tcatctctgc atggatcgtt cgcaagcagt gtccatccac
     7741 gacgtaacac ccttcatcta tgcagtggac atctccactg actgctccat tatagccgcc
     7801 agcaatgatc cttttttctc gaacgagtgt cgctcctact tctaaacgtg tacacgtact
     7861 acgtaaagat aacagtactg cctgagccat aaagtactgg tcccaaggaa tgcgttcatt
     7921 atgtgactta tcagtaactt tttctttatt cgtatcgcat gcttttgaag atttttctaa
     7981 catgttttat attcctccgt tttttcaagt tgattaaaaa gtactcattg cttaaacaaa
     8041 atattcttta aatataacga atcatcctgt ctgacaaaag atcctaccct tcactgccga
     8101 aatgaagtcc gtcatccatt agcccattat aaaaattctt cgaatccatt tgttcaagaa
     8161 attccgtata aaaatcgata cttcacaatc atggcgtttt gctacttctg tgagaacaga
     8221 gatatattct ttgatcaact tgttattccg atatttttgt tttgtctcat cgactgctgg
     8281 tggcgtgata aagaaagcat gatcagaact atagtctttt ttcagctgaa ctatcatcca
     8341 ctgcagattt tttggtacgt ttctttagaa agttgtttat gctctgctag atcattcgtc
     8401 ccaatgagaa taaatacttt atctgctgta tagctaagta catctttggc aaagcgttct
     8461 cgtagagctt gactgttatt gccgctgaca gccgtattca taatatgaat cccagatatt
     8521 ttattttgga gatagacatt cagcattggt ttttttaatg tttctttgcg aacaatcaag
     8581 ctatttccta tccaaaaata ctacctcctc gtcctgccaa gctcttagaa ttggattatt
     8641 cttagtatcc attagcgtcc atttattgct cgtgccagtc gattttgaat cattctattt
     8701 aattgtaacg aagtttttta gtttttcaac tgtcttttca ccgataccag aaatctcctg
     8761 caggtcttcc actttctgga aggatccgtt ttcttctcta aatcgaatga tttcttgcgc
     8821 tttgactgat ccgatcccag gaatctgctg aagttctgat ggagtggctg tattgatatt
     8881 tattttggga ggctcggaat ctgctgtagg ctgttttcct tccgaggatg ccaactgatc
     8941 aagatgttct ggcagctctt cacctacagc agggatatat aggacttgct gatcttctat
     9001 cttcgcagaa aagttgattc gatcgctatc cgcttcttct aataatccac ctgcacgctt
     9061 tagtacatca tagacccgtt cttctgaaga aaaggaatag ataccaggct ccttcacact
     9121 tcctttgata tcggtgtaca tgacctttgg acgagcatcc tcttctacat cgtttccttc
     9181 agctgatgga tcttctccac tggaactaaa cgaagaaagt atttcttttg cagcagtctc
     9241 ttctgtttct ctataaaata gcaaatttga taataaaaaa ccacttgcga tacaaaagat
     9301 aagaactatc atagacatcc aaatgatcaa tttgggtgaa gctagtattt tcttgctgat
     9361 tttttctatc attgttcttt tttcaaagaa tgaccagtaa aaatcattca ttactccttt
     9421 catttaaaat tgaagaaaca gtgagctctg gcaaaatcgt cccttcaaca tctaattacg
     9481 tcaatttttt cttttgtttt tatttttatc gt
//
LOCUS       DAFCLG010000099         9484 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFCLG010000099 DAFCLG010000000
VERSION     DAFCLG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.099"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..360
                     /locus_tag="LND56_002177"
     CDS             4..360
                     /locus_tag="LND56_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBK6744056.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="LND56_002178"
     CDS             376..726
                     /locus_tag="LND56_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBK6744057.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="LND56_002179"
     CDS             755..1534
                     /locus_tag="LND56_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBK6744058.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="LND56_002180"
     CDS             1545..2222
                     /locus_tag="LND56_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBK6744059.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="LND56_002181"
     CDS             2241..3347
                     /locus_tag="LND56_002181"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBK6744060.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="LND56_002182"
     CDS             3337..4428
                     /locus_tag="LND56_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBK6744061.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="LND56_002183"
     CDS             4441..5181
                     /locus_tag="LND56_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBK6744062.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5469..7409
                     /locus_tag="LND56_002184"
     CDS             5469..7409
                     /locus_tag="LND56_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBK6744063.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7496..9418
                     /locus_tag="LND56_002185"
     CDS             7496..9418
                     /locus_tag="LND56_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBK6744064.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         3117 a         1574 c         2122 g         2671 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta actcattaga aaataagaag cttattccag aatgaaatta
     5221 aatggaataa gctttttata attaattacg gaaattgtta atgtagataa taatttagat
     5281 gtacgtaagc agaagtacag aaatgtaaga ttttccaata ttattttatt caacataata
     5341 aatcgtatat caattttttt agcgaaattt ctatttattc caagaatttc agttattacg
     5401 gaatcatttt gtgcttaata aaaagaaacc gtttttatat actataaatg tagaaaggcg
     5461 gtaggaaaat gaaaaaaatt attgcaatca catcctgtcc agttggtatt gcccatacat
     5521 acatggcagc agaaaactta gagaaagcgg ggaaagcagt tggcgccgag gtaaaagtcg
     5581 aaacacatgg ttccatcggt attgaaaatg aattgactgc aagagatatc gaagaagcag
     5641 ctggtgtaat catcgcagca gatactaaaa tagataaatc acgattcggc ggtaaacctt
     5701 tgataaccgt aggtgtgcaa gaaggaatcc accatgcaga tgagttggtg agagatattt
     5761 tagcaggaaa agcgccagtc tataaaagta cggaggcaat tatttcttcc gaaaataaaa
     5821 gtgaatcaga aaatttaggt aaaaaaatct ataagagttt gatgaatggc gtttcgtaca
     5881 tggtaccttt cgtagttaca ggagggttat tgatcgcgat ttctttaaca ttaggaggaa
     5941 cagctacacc tgaagggatc aagataccag aaggaacgat ttgggcaacg atgaatagta
     6001 tcggaagtat cgctatgggg ttgatggttc caattctatc tgcatttatt gcgcaaagta
     6061 ttgcagatcg tccaggactt gttcctggat ttgttggtgg gatgttagcg gcaaacggag
     6121 cattatatgg tagtgatgcg aatgcgggat tcttaggcgg gatcatcact ggatttttag
     6181 caggattcat tgctttagga atcaagaaag tgcgagtacc gaaagcttta caatcgatca
     6241 tgccaattat tgttatccca attttaggat cattggcagt tggttttgtg tttatctatg
     6301 ttattggaga accagtagct cttctattta gttcattgac tcacttttta gcagggatgc
     6361 aaggaggaag tgaaattgtt ctagctatga tcttaggagc gatgattgct tttgatatgg
     6421 gggggccgtt caataaagta gcctttttgt tcggatctgg cttgattgca gaaggcaatt
     6481 attccattat ggggccaatt gctgtagcta tctgtatccc tccattagca ttggggctgg
     6541 catcattgat tttaaaaacc aaatttacga aaacagaacg agaagctggt aaggcgtcct
     6601 tagcaatggg actgtttggg attacagaag gcgctattcc gtttgcttca acagatccac
     6661 tacgggtgat tccaagcatt gtaatcgggg caatgacagg aagtgtgatc gcgatgcttt
     6721 caggagtgac agatcatgta gctcatggcg gtccgatcgt agcggtttta ggagcagtgg
     6781 atcacgtagc catgtttttt gtagcagtga ttgctggtat tgctgtaacc gtgctaatgt
     6841 taagaatcct taaaccgaca ttacaacaaa ctgcggttgc tgcagcaaat ggtgtgacag
     6901 tagcagatct atcagagact gaaacaagtg atgtttatac gaaaactgaa acttttgatt
     6961 ctatcacgga tattatgtca gatgaagcaa tcgttttagg tgaaacagca acaacaaaag
     7021 acggagtaat cgaagattta gtccaacgtt tagaaaaaac agcggcgatc acagataaaa
     7081 ccagatttaa agaagtcatc tatgatagag aacatgaaag tacaacggga atcggtatgg
     7141 ggattgctat tccacatgga aaatcagctt atgttcttaa accaacagtg gcatttgcca
     7201 gaagtcaatc tggggtagat tggcattctt tagatggaaa acctgcacat atgatcttta
     7261 tgattgctgt acctgaaaac agccaaggtg atatgcactt gaaaatactg caacgtctat
     7321 cacgtaaatt gatggatgat gacttccgtc aagcactgat ggaagcgcca gataaaacaa
     7381 cagttcacca attattaagc gaaatgtaac ataagatgaa aaatcgaccg tgatgagaca
     7441 taatagatgt gttatcatga tcgatttttt atttagaagg gagtttctgc acattatgaa
     7501 tcagcgtcag caatctttga tctatcaatt aagtgagaac aataattata taacaacaaa
     7561 aaagctagca gagcatttcc atgtgtcaga aagaacgatt catagcgatt tgaataccat
     7621 tgaaacttgg ttgagagaca aacatctgcc tttaatgttt gaaagaaaaa aaggtacagg
     7681 tatcttgttg aatggtctgc ctcacgaaag ggaacaactg aaacgagcgc ttaatgaaga
     7741 gactcaaaca gaaatggata aaaaacagct tcgtcagtta ttgattttcc atctgctagt
     7801 agcaaaagac ggatttagtc ttgaagaact ttcagaaaaa ctatatgttg gaaaacgtac
     7861 gattcgtaaa gaattgacag agttgcaaag cttttttgag tatcaccatt tacagctagt
     7921 atccaaaaca aaactaggaa cttttcttaa aggcgatgaa caagaaaaaa gacagttgct
     7981 tgttaagaca ttacgaaata tgcagaaaga agatccacaa agtccgtcgt tgaaagaatt
     8041 cttcgctaaa gatacattaa aagttatcca acatacatta caagatgttt tttatgaaaa
     8101 ccatttagaa cagccgaatg gactggctag tgtggagatt cacatctact ttatgctcga
     8161 gcgaatgaaa cagtaccaga aagtgaaatt aagtaaagac gaaaacgaag tcgtggaaca
     8221 cacccaagca caaaagttaa gtagtcaaat attagcaaaa ctagccacca tctatccaat
     8281 tgaattttct cctgatgaaa tcaattatct ggctttgcgt atcgccaatc tttttacaaa
     8341 ttcacaagca gccacacgtt ttcaaaagga atctagcgca cttgctgatc atttgatcga
     8401 acaagtggaa cagtttctag gctattcatt aaaagaagat cagctactaa aacaaaattt
     8461 aaggtctcat ttatcttcaa cttattttcg actccattat ggtttgcaga tttctaatcc
     8521 attgacgaaa aatgtgttta gtacgtacac acaacttttc ttagttttac agttaatttt
     8581 agaagattat tttgaaaagg aaaattttta tgttccacaa gaggagacag cttatttaac
     8641 gatccatttc caagcagcga tcgagcgaca caaacatcag aaaagtagaa gatatcaaac
     8701 ggttttagtt agtgaatatt caaaagctat ggctactttt ctcgaagcac ggctgaacag
     8761 agaacttcca gaattaacga ttatggatct agttgaatat aagccagaat taaagagaca
     8821 agattttccc tctgttgatt ttatcttggc tacgttacca tttaaacata taagcattcc
     8881 agtaatcgag atctcaccaa tgatcacaga aactgatcta gcctatttga caaaatatat
     8941 gctggagcat gtgcctatca agaagaaaaa aacatttgat ctagcaagct ttactcatcc
     9001 atttctgatc tttcctcaac ttgaacggac agacccagcc gatattttaa actttatggg
     9061 aaacgtctta gtggaacatc attatgtgga gccggaattc atcgattctg tcttggaaag
     9121 agacagtcac tccagtacta gagtagcacc atttgtaaca atccctcacg gtaatccgct
     9181 gtatgtgaag cattcgatga tttctattgc tacaatgaaa gaaccagtcc tatggcacgg
     9241 ggaacagata cgtatcgtac tgctattagc aataaagaaa aatgatttga aacatgcaga
     9301 gttcaaaaaa atattttctg ttattcacta tctagaaaag cagccagaac aaatggctca
     9361 gttgatgcaa tccaatcatc cgctagaaat cttaacgttg ttatctagtt atgaataagt
     9421 agcgattaaa aaactgcctt tatatttata ataattctat ctatttattt ggaagtagaa
     9481 aact
//
LOCUS       DAFCLG010000100         9454 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFCLG010000100 DAFCLG010000000
VERSION     DAFCLG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.100"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..538
                     /locus_tag="LND56_002186"
     CDS             11..538
                     /locus_tag="LND56_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK6744065.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="LND56_002187"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="LND56_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK6744066.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="LND56_002188"
     CDS             2005..3162
                     /locus_tag="LND56_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBK6744067.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /locus_tag="LND56_002189"
     CDS             3332..4792
                     /locus_tag="LND56_002189"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK6744068.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    4789..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..5650
                     /gene="cysE"
                     /locus_tag="LND56_002190"
     CDS             5114..5650
                     /gene="cysE"
                     /locus_tag="LND56_002190"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK6744069.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /locus_tag="LND56_002191"
     CDS             5647..7059
                     /locus_tag="LND56_002191"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK6744070.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="LND56_002192"
     CDS             7056..7451
                     /locus_tag="LND56_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBK6744071.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="LND56_002193"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="LND56_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK6744072.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="LND56_002194"
     CDS             8308..8838
                     /locus_tag="LND56_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBK6744073.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..>9454
                     /locus_tag="LND56_002195"
     CDS             8915..>9454
                     /locus_tag="LND56_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6744074.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKL"
BASE COUNT         3223 a         1724 c         2136 g         2371 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattatgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaatcg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tacaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgagcctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcatta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcgattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaagcta aaatttttca
     9361 ttattatatg caaggcgttg attttgtaga catggctgaa tgtttagcaa tggatcacaa
     9421 gcaagtaatg agcgcttacg ggagagtaaa actc
//
LOCUS       DAFCLG010000101         9434 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFCLG010000101 DAFCLG010000000
VERSION     DAFCLG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.101"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            167..361
                     /locus_tag="LND56_002196"
     CDS             167..361
                     /locus_tag="LND56_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744075.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..929
                     /locus_tag="LND56_002197"
     CDS             630..929
                     /locus_tag="LND56_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744076.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1026..1460)
                     /locus_tag="LND56_002198"
     CDS             complement(1026..1460)
                     /locus_tag="LND56_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6744077.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="LND56_002199"
     CDS             complement(1692..2402)
                     /locus_tag="LND56_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6744078.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="LND56_002200"
     CDS             complement(2392..3252)
                     /locus_tag="LND56_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK6744079.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="LND56_002201"
     CDS             complement(3257..3895)
                     /locus_tag="LND56_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK6744080.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="LND56_002202"
     CDS             complement(3910..4209)
                     /locus_tag="LND56_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6744081.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="LND56_002203"
     CDS             complement(4252..5736)
                     /locus_tag="LND56_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6744082.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="LND56_002204"
     CDS             complement(5756..6217)
                     /locus_tag="LND56_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6744083.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LND56_002205"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LND56_002205"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK6744084.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="LND56_002206"
     CDS             7520..8290
                     /locus_tag="LND56_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6744085.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="LND56_002207"
     CDS             complement(8563..9390)
                     /locus_tag="LND56_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744086.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2930 a         1932 c         1480 g         3092 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcat
//
LOCUS       DAFCLG010000102         9387 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFCLG010000102 DAFCLG010000000
VERSION     DAFCLG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.102"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            236..1042
                     /locus_tag="LND56_002208"
     CDS             236..1042
                     /locus_tag="LND56_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6744087.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="LND56_002209"
     CDS             1054..2274
                     /locus_tag="LND56_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6744088.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="LND56_002210"
     CDS             2264..3850
                     /locus_tag="LND56_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6744089.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="LND56_002211"
     CDS             3889..6027
                     /locus_tag="LND56_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6744090.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="LND56_002212"
     CDS             6315..7706
                     /locus_tag="LND56_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6744091.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="LND56_002213"
     CDS             7765..8574
                     /locus_tag="LND56_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6744092.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..>9387
                     /locus_tag="LND56_002214"
     CDS             8546..>9387
                     /locus_tag="LND56_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6744093.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKY"
BASE COUNT         3234 a         1442 c         1895 g         2816 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ctcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa aatatta
//
LOCUS       DAFCLG010000103         9275 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFCLG010000103 DAFCLG010000000
VERSION     DAFCLG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.103"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..435
                     /locus_tag="LND56_002215"
     CDS             <1..435
                     /locus_tag="LND56_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK6744094.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="LND56_002216"
     tRNA            complement(759..842)
                     /locus_tag="LND56_002216"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="LND56_002217"
     CDS             1370..1870
                     /locus_tag="LND56_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6744095.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="LND56_002218"
     CDS             complement(2060..2404)
                     /locus_tag="LND56_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBK6744096.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="LND56_002219"
     CDS             complement(2558..3478)
                     /locus_tag="LND56_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6744097.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="LND56_002220"
     CDS             3665..4156
                     /locus_tag="LND56_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBK6744098.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="LND56_002221"
     CDS             complement(4180..6555)
                     /locus_tag="LND56_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK6744099.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="LND56_002222"
     CDS             6881..7507
                     /locus_tag="LND56_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6744100.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="LND56_002223"
     CDS             7530..8234
                     /locus_tag="LND56_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6744101.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="LND56_002224"
     CDS             8426..9259
                     /locus_tag="LND56_002224"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK6744102.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1789 c         1651 g         3007 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acact
//
LOCUS       DAFCLG010000104         9054 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFCLG010000104 DAFCLG010000000
VERSION     DAFCLG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.104"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            171..989
                     /gene="purR"
                     /locus_tag="LND56_002225"
     CDS             171..989
                     /gene="purR"
                     /locus_tag="LND56_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBK6744103.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1492..2865
                     /gene="glmU"
                     /locus_tag="LND56_002226"
     CDS             1492..2865
                     /gene="glmU"
                     /locus_tag="LND56_002226"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK6744104.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2959..3930
                     /locus_tag="LND56_002227"
     CDS             2959..3930
                     /locus_tag="LND56_002227"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6744105.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3936..4038
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4104..4673
                     /gene="thiT"
                     /locus_tag="LND56_002228"
     CDS             4104..4673
                     /gene="thiT"
                     /locus_tag="LND56_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBK6744106.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4754..5527)
                     /locus_tag="LND56_002229"
     CDS             complement(4754..5527)
                     /locus_tag="LND56_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744107.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5794..7494
                     /locus_tag="LND56_002230"
     CDS             5794..7494
                     /locus_tag="LND56_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK6744108.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7464..7682
                     /locus_tag="LND56_002231"
     CDS             7464..7682
                     /locus_tag="LND56_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBK6744109.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7702..8592
                     /locus_tag="LND56_002232"
     CDS             7702..8592
                     /locus_tag="LND56_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK6744110.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8610..8894
                     /locus_tag="LND56_002233"
     CDS             8610..8894
                     /locus_tag="LND56_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744111.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3062 a         1543 c         1894 g         2555 t
ORIGIN      
        1 ctaaattata aagaaaagaa gccttataaa aacgaaaaat tttttatagg cttcttttta
       61 ttttaataaa taccgaacta ttttcttatt ttttcataaa atgctcgaat ttttgaagaa
      121 aaacttatat aatgtaaaac gataaaattt cttaaaagga gttggaatag gtgaaagtac
      181 gtcgcagtga acgtctagtt gacatgacac agtatttatt gaatcacccg catgaattga
      241 tttccttaac aagctttgcc gaacgttatg aatctgctaa atcttctatc agtgaagatt
      301 tgggaatcat caagaaaaca tttaaagaac gagggtatgg aaccttggaa acaatcccgg
      361 gtgctgcagg tggtgtattg ttcatccctg aaatttcata tgaagaggcg aaaaaatata
      421 ttgaatctct agcagaacgt ctatcagaac aagaccgtct gcttccagga ggatatgttt
      481 atctctctga tttgttagga gaacctgaac tgctgcgtca agttggaaga atcattgctt
      541 ctaaatacct tggtgaaaaa atcgatgctg taatgactgt agccacaaaa ggcgtaccta
      601 ttgcccaagc tgtgtcctat tatctgaatg cgccattcgt tattgtacgc cgggactcaa
      661 aaataacaga aggatcaaca gtaagtgtca actatgtgtc tggatcttct gaaagaattg
      721 aaaagatgga actgtctaag agaagtttga aacgtggttc acgtgtcctt gtagtggatg
      781 atttcatgaa aggcggcgga acagtcaacg gaatgaagag cttgattgaa gaattcgaat
      841 cagaacttgt tggcatcact gtatttgccg aatcaaaatt caacggtcat cgcgccatcg
      901 atgattatac gtcactgctt tatgtcaaag atgtagatac tcaaacgaaa aatattacgg
      961 ttgtgccagg aaattatttt tcagaataag aggttgtgaa aaagctgctt agtttcgagt
     1021 agtaagaaaa attttgtgaa aatggctttc cacatttttt cataaatttg gcttaatacc
     1081 gagaaactgg cttttgaaca ccgtttatct agaggttgtg aaatcagcgt cctgtcctga
     1141 gtattagctc aataaacgga aaaattgctc ctcatatttt gccgtttatt gagctaatac
     1201 cgaaggtcag ctgattgaaa aacgtttaat gaagaggttg taagcctacc gtttaactct
     1261 aagtcacaag aaacgatttc aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt
     1321 gaccgaggag ttggcaggag agcaccggtt accaaataga taggcccgtt atggtctatc
     1381 tattttttta tccagaaaac gctgggataa aagaaaatca cttgttttgt tgaaaaaatg
     1441 ggtatactgg gataaactga aatcagcaaa ctatatttag gagtgacgca gttggacgca
     1501 cgttatgcaa ttattttggc ggcagggaaa ggtacccgca tgaagtcaaa attatataaa
     1561 gttttacatc ccgtttctgg taaacctatg gtagaacata tcattaatcg ggtcagtgaa
     1621 acgaaaccag atgaagtgat tacgatcgtt ggacacggag cagaacaagt taaagcacaa
     1681 ctaggagaac gcagtaaata tgcgttacaa gcagaacaat tgggaacagg acatgctgtt
     1741 ttgcaagctg cttctttctt ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat
     1801 acaccattat tgactactga aacgctgaac aatctttttg aatatcacca aggaaaaaat
     1861 gcaagtgcaa cgatcttgac ggcacaagca gaaaacccaa caggatatgg acgaatcatc
     1921 cgtgaccata ttgggatcgt agaaaaaatc gtggaacaaa aagatgcaac accagaagaa
     1981 gctcttgtac aagaaatcaa tacaggaact tactgttttg acaacgaagc tttgttcgat
     2041 gcgttaagca aagttggaac aaacaatgct caaggtgaat actatctaac agatatcatc
     2101 gaaatcttaa aagaagaagg ccatacagtt gctgcttatc aaacggatga ttttgaagaa
     2161 tcaatgggag tcaatgatcg gatcgcgtta gctaaagcaa atgagatcat gagaaagcga
     2221 attaaccaga tgcatatggt caatggcgta agctttgtcg attcagcaac gacatacatc
     2281 gatgcaggcg tagaaatcgg tccagacaca ttgatcgaag caggtgtaca aatccaagga
     2341 aatacagtca ttggttctga ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg
     2401 attgaggatc acgtcgtcat tgaaaactca gtcattgaaa gcagccatgt caagaaacat
     2461 gcagatgtgg gaccttatgc acatttacga ccaaaagctg aaattggcga aaacgtccat
     2521 atcggaaact ttgtggaagt gaaaaatgct caaatcggca aaggaactaa agtcggtcac
     2581 ttgacttatg ttggtgatgc tacgctggga gaagaaatca atgtcggatg tggtgttgtc
     2641 ttcgtcaact atgatggaaa gaacaaacat catacgactg tcggcgacca cagctttatt
     2701 ggttctagca cgaatatcat cggacctgtc gaggtagcta aaaacagttc gattgctgcc
     2761 gggtcaacaa ttacagacaa tattccagaa tatgcgctgg caattgcgag agcaagacaa
     2821 gtcaataaag aaggatatgc aaaaaaactc ccttatctaa actaagactt ttgcaaaatc
     2881 acttgattta tttaaaaaat atgactacta tttattaggt agaattctag agttgtgaaa
     2941 aagaaagtgg aggtccccat gtcaaaacat tactttgacc caagattaaa aatttttgcg
     3001 ttaaattcca atcgcccatt agcagagaaa attgcagcag cagtcggcgt cgagttagga
     3061 aaatcatctg ttactcaatt tagtgatgga gaaattcaag taaacattga agaaagtatt
     3121 cgtggatcgc atgtctatgt tattcaatca acgagcagcc cagtcaatga taacttgatg
     3181 gagttgctga tcatgattga cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc
     3241 atgccttatt atggctatgc gcgtcaagac cgtaaagcac gtgcacggga accaatcaca
     3301 gcgaaattag tagcaaacat gattcaaaaa gctggtgcaa caagaatgct gactttggac
     3361 cttcatgcag ttcaaatcca aggtttcttt gatattccag tcgatcattt gatgggtgcg
     3421 ccacttattg ccaattattt cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt
     3481 tcaccagacc atggtggtgt cacacgggca cgtaaattag cggaattttt gaaatctccg
     3541 attgcgatca tagacaaacg ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt
     3601 ggacaagtca aaggcaaaac ttgtgtactg atcgatgata tgatcgatac agcaggtacg
     3661 atcacattgg ctgccaatgc tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt
     3721 acacatccag tattatccgg ccctgcttta caaagaatag aagattcagc tatcgagcgt
     3781 ctagttgtta cagactcgat ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa
     3841 gtaagtgttg gcggactaat gggagatgca atcaaacgta tccatgaaaa caaaccagtt
     3901 agtccattgt ttgaaacaaa aaataaataa taaataaact cacaaagggg agtccaattg
     3961 ggctgagatt gaatctattt ctaaaccctt cgtacctgta tcggttatgc gagcgtagga
     4021 attgtgaata aacagctgtt tgactatttt cgaaagctgc ttcctccttt gtgacttttt
     4081 tattcaaagg aggaagtttt tttatgggaa aaaatagagt ttgggtagaa ggaacgattg
     4141 ttgcagcgtt agcgatggtg ttgtcgttga ttccgatcca gatcggcagc agtttttcta
     4201 tttcattagg gcagattcca ttgactcttt ttgcgttaag aagagggaca aaggcaggga
     4261 tgctggcagg tttcatttgg ggcatcctac attttccgtt gggacaagtt tattatttaa
     4321 gtgtcgttca agtgttgatt gagtacccga ttgcctatac ttttgcaggc gcagcagggc
     4381 tcatggcttc aaatgtccaa aaaggctatg tagacgataa tacgaaacaa attagaaaaa
     4441 gcatcgtgtt tggtgcattg attggcgcac tttccagata tttttggcat tttattgcag
     4501 gagtggtttt ctggggagcg tatgcactat gggggatgaa tccttggctc ttttctttcg
     4561 taatgaatgg tgccagcggg ttgagtacag ctattgtaac gatcatcgtt ttattaggga
     4621 tccaacaaac gactccttcg atttttgtgc cagataaacg acaggagctg taaataaaaa
     4681 agaagcaaga aacttgagac agaatcatgt cctagtttct tgtttccttt ttattttttg
     4741 ataagaaata gatctacttc aatggcagat aagaaagatc ggcatcttct accaattgcc
     4801 agtgtcgatc ggtgacagag tagattaaag tagcgatgat ttcctcttct cgataccggg
     4861 tgccttttaa tttccaaata taatcttcaa tcacatagat tgggatacga tatttaccat
     4921 cgatcaaacc aacttgatca aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt
     4981 ctttcaataa ttccacactg attttttcaa caggtgtttc ttttttgaaa aaagattttt
     5041 ttacattgat cttatctgac aagatcgaac ttaatttact agcaaaaatc ctataaaatt
     5101 catctaaata gcgataataa atccgattca gctgatcatt aagatcgaag tatacaaaat
     5161 tattctgtag tttataaaaa aacggcgaat gaagatgtgt tttcatatga ccaaaatata
     5221 ataattctga aatttctaaa ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga
     5281 tccacttcaa ttcatcgaat ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat
     5341 tttctgtacc acggattact ttcagccctg tatgggattc atattctcca gcagaagcag
     5401 atggatcaag caagagcaag ttttgaggaa aatgcacgat actgtttgca aagtcagtag
     5461 cagtcagccc ttttgttaaa acagcattac ttgtggtatc aatatgtaca taaactgact
     5521 cagtcatcat gttcctcctc ttatttcctc ttaatctctt ttattttaca acatttgcat
     5581 taaaaaagct tgtttctttt attttaaaat tccattacaa aatcacgttt tcattgaaaa
     5641 cactctcatt ttcaattaat ttttgtgatt tttgtatttt tttatttaaa ggaggtttta
     5701 tgaattgttt tcttatggaa tctacaaagt cgaatacgag actcatgacc tttttatgac
     5761 gtactggtat acttgaagaa aaaggagagc gcgatggaga taaaaaaagt ttacttttca
     5821 cagaaaatgt cgctgtattc gcctatccag caagcaaaac aaccccttcc attttttcaa
     5881 agtcatcaag aaagtagaga cgctttttgg cgttctgcaa aaaagcaatg tgcttttaaa
     5941 gcagtatcga aaaaatatac cgtctatttt actattgcta aaagtagagg agatgttatt
     6001 tttaataatc tgttgattga aggtgaaact tttgaatgga aaaaattttt tcggtatatg
     6061 gaagcttgcg ctcgtttttt tattaaagaa aactgttgtt ttttatttca atctcctcta
     6121 tcagaagaat ggttgcgtat ttttcataaa aacggctatc aaggaaccac acaattaaat
     6181 aaaaagctgg tctatcatac agcacttgtt ttaggtggag gaggagcgca cggtgcttat
     6241 cagattggtg tgtggcaggc gctgaaggaa catgatatca actttgagat tatcacaggt
     6301 acctcggtag gtgcgttgaa cggcgcattg attttacaag gagatatgaa aaaagctgtg
     6361 aatttatgga aaaagctgtc gacaagacaa gtgcttgcgt taccagaaat ggctgcagtt
     6421 gaagaccttc gtgaacgctt ctatcgagaa agacgccaaa tgacgaaaac agcactaatt
     6481 gaaggcgggg tttcttctgc tccattggaa aagatggtca aagacaattt ggatttgtcc
     6541 ttactcaaac ataatcccaa aatacgttta tatacggtgt ctactaaact gccagaacta
     6601 gcggaagtag tgactgatat ccaacagaca aaaacagaag aaataccaga ttggatactc
     6661 gcctctgctt cattttatcc cgctatggct tatcgaaaga tcggaaaaaa taaatatgca
     6721 gacggtgggt atcgcaacaa aatacctatt gatattgcga ttaacaaagg agcaacagag
     6781 gcatttgtcg tagatgtgca agggccaggc ccagcgaaaa aaattcgtat gccagacatt
     6841 tttatccatt ggaaatgtca aacattgtgg acactaggca gctttctgct ttttgatagc
     6901 caaaggaacc aattaaattt acaattaggc tatctggaaa tgaaaaaacg attaggctat
     6961 tactatggga actggtatac atttcattct gttaaacctg caagaacata ctggcgagga
     7021 tttttgtctt acttaacaaa agaaattcag ttagaattat cttttttcaa aagcataaaa
     7081 ttttggcaaa aactgcgtaa tttatataag aacagggtcg taccagagac atgcggattg
     7141 gcgatgctag aacttttagc aaaaaaacat tttttactgc ccaatgaaat ctatcaagta
     7201 gacatgatga ttcaaatgat ctgccaacaa gatataaaaa gtatgccgga tgatttattg
     7261 gcacaaattg gtcaattaag tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa
     7321 atacaaaatg agcgcacgaa aactgtttat tttgattatc tgctacaagg aaaacgcaag
     7381 gatcgtttgc agcatgaatt gcttaagcaa ccagttgaca cattactgat cctatattta
     7441 tattatctca aggaggaaca accatggcac aagaattttc atatgaaatc gtagaagaaa
     7501 tcgctatatt gtcagaaaac aacaaggggt ggcgtaaaga actgaaccta gtcagctgga
     7561 atggtcgccc gccaaaattc gatcttcgag attgggcgcc agaccatgaa aaaatgggga
     7621 aaggcctgac attgacgaac gaagagtttg aacaactgca aaaagcaata gaaaatatgt
     7681 aatgaggaag gaccctgtcg aatgaaaagt atgactggtt ttggcaaagc catccgcgaa
     7741 acaaaagcgt atcagttaga tattgaaata aaaagtgtca accagcgttt tctagatgtt
     7801 caaatacgaa gtcctaaaat cctgaacttt gttgaaaacg attttcgtca gcttattaaa
     7861 cagcagttaa gccgcggtcg aatcgaagta tttgtgaatc tagcttatat tggcaaaaac
     7921 gaaaaacaga tcgtcatcaa ctgggaattg attgattcac ttatcactca attgacagac
     7981 ggaattcaac aacgatacgg tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc
     8041 ttcgcgctgg acgaaaactt cgtaacggta gaagaaaaac cggaagagag catggaagaa
     8101 ttggcacagt tagctctaga tacattgaaa agcgcacttg attctattga ggcgagtcga
     8161 gaaaaagaag gtcaagcttt agctttagtg ttgaaaaaga atactgaaga attcaaagaa
     8221 gtactaaaaa aactcgatag ttttgtagaa atctatgaaa aggaatttca aataaggtat
     8281 caaaaaaagc tggaggaatt tcttggagcg aaagtcgatg agcaacggtt gttgacggag
     8341 ctagccatct tgttggaacg cggagatatt cacgaggaac tggatcgtct ggcgatccat
     8401 gtacaaaaac tagatgaact tatcgaagca caatgtcctg taggtcggga attagatttc
     8461 ctgatccaag aaatgaatcg ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa
     8521 attaaaaatc aagtcgtcca gtctaagacg atcttagaaa aaatcagaga acaagtacaa
     8581 aatattgaat gacgaaagag gtagagataa tgaaatatta tgacgtaacg ttccacgaat
     8641 taagtggaaa aactgtggtt aaacgggata tcccttctga aaaaaacggc tttgacgtat
     8701 ggaaagatgc ctgtgcagac tataatgaga acgaactgtt tattttgatc aacgacggtg
     8761 cttacgtgac gatgaatcgg aaatttattg ttcgaattga cacagaagag gtagaagatc
     8821 cgacagaaaa agcacgttct cgaaaagatg agatcatggg tgtagtgaat actttgtcca
     8881 atatgggctt ttagcaagga tgctgaacaa agaaatacaa taaataatca gaattggatt
     8941 tcttcgctta gcttctttgc tctaggaata catttaaagg ttgtgacaaa attcgtgtca
     9001 caatcttttt ttattttgaa taagtgatgt tcaaaaacca ttttctcggt atta
//
LOCUS       DAFCLG010000105         8884 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFCLG010000105 DAFCLG010000000
VERSION     DAFCLG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.105"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            122..1462
                     /locus_tag="LND56_002234"
     CDS             122..1462
                     /locus_tag="LND56_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6744112.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1680..2123
                     /locus_tag="LND56_002235"
     CDS             1680..2123
                     /locus_tag="LND56_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK6744113.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2627..4045)
                     /gene="nhaC"
                     /locus_tag="LND56_002236"
     CDS             complement(2627..4045)
                     /gene="nhaC"
                     /locus_tag="LND56_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6744114.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFYVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4448..5671)
                     /locus_tag="LND56_002237"
     CDS             complement(4448..5671)
                     /locus_tag="LND56_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBK6744115.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5702..6541)
                     /locus_tag="LND56_002238"
     CDS             complement(5702..6541)
                     /locus_tag="LND56_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6744116.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6558..7238)
                     /locus_tag="LND56_002239"
     CDS             complement(6558..7238)
                     /locus_tag="LND56_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6744117.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7240..8313)
                     /locus_tag="LND56_002240"
     CDS             complement(7240..8313)
                     /locus_tag="LND56_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6744118.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8369..8607)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8650..8883)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2544 a         1816 c         1613 g         2911 t
ORIGIN      
        1 cctaaaagtt ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta
       61 aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt
      121 tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt
      181 aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg
      241 cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg
      301 tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg
      361 cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat
      421 tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca
      481 cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta
      541 ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat
      601 tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt
      661 tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg
      721 aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat
      781 cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat
      841 gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg
      901 gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat
      961 cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa
     1021 ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc
     1081 ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg
     1141 taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg
     1201 ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc
     1261 atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc
     1321 attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac
     1381 tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc
     1441 gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga
     1501 tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata
     1561 aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat
     1621 tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa
     1681 tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta
     1741 tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca
     1801 tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt
     1861 atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt
     1921 ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga
     1981 ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag
     2041 taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa
     2101 aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag
     2161 aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt
     2221 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat
     2281 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
     2341 gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca
     2401 tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga
     2461 ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca
     2521 catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt
     2581 aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt
     2641 gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg
     2701 tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt
     2761 aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca
     2821 ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa
     2881 atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt
     2941 gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg
     3001 gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga
     3061 tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt
     3121 cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc
     3181 ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca
     3241 ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg
     3301 cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt
     3361 ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta
     3421 aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct
     3481 tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata
     3541 atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg
     3601 gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacat
     3661 aaaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa
     3721 accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt
     3781 gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg
     3841 gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg
     3901 catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc
     3961 cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa
     4021 aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct
     4081 aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa
     4141 aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa
     4201 acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg
     4261 tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc
     4321 tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa
     4381 aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta
     4441 ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt
     4501 gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta
     4561 gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt
     4621 cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta
     4681 tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta
     4741 tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag
     4801 gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg
     4861 tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt
     4921 gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag
     4981 ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta
     5041 gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact cctttttcag
     5101 gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta
     5161 aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat
     5221 cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt
     5281 cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta
     5341 tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac
     5401 ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat
     5461 aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt
     5521 tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat
     5581 gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg attagatcct
     5641 tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt
     5701 cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga
     5761 ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt
     5821 tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg
     5881 gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat
     5941 tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag
     6001 aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg
     6061 ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg
     6121 taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag
     6181 tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc
     6241 cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg
     6301 atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat
     6361 taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa
     6421 taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac
     6481 accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca
     6541 ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact gataaattga
     6601 cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat
     6661 cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc
     6721 atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc
     6781 aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct
     6841 gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa
     6901 aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca
     6961 actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga
     7021 atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta
     7081 gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct
     7141 gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa
     7201 taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac
     7261 tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat
     7321 caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact
     7381 ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg
     7441 atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa
     7501 ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc
     7561 taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg
     7621 attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc
     7681 taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa
     7741 acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc
     7801 aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc
     7861 gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc
     7921 ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt
     7981 tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt
     8041 gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag
     8101 ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt
     8161 catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc
     8221 gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt
     8281 gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc
     8341 gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc
     8401 atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata
     8461 tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt
     8521 tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta
     8581 tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa
     8641 ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct
     8701 caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa
     8761 tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc
     8821 ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat
     8881 cgat
//
LOCUS       DAFCLG010000106         8806 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFCLG010000106 DAFCLG010000000
VERSION     DAFCLG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.106"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..241
                     /locus_tag="LND56_002241"
     CDS             <1..241
                     /locus_tag="LND56_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBK6744119.1"
                     /translation="KLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRH
                     SLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            308..802
                     /locus_tag="LND56_002242"
     CDS             308..802
                     /locus_tag="LND56_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6744120.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            858..1496
                     /locus_tag="LND56_002243"
     CDS             858..1496
                     /locus_tag="LND56_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744121.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1568..1963
                     /locus_tag="LND56_002244"
     CDS             1568..1963
                     /locus_tag="LND56_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6744122.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1947..4400
                     /locus_tag="LND56_002245"
     CDS             1947..4400
                     /locus_tag="LND56_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6744123.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4397..6424
                     /locus_tag="LND56_002246"
     CDS             4397..6424
                     /locus_tag="LND56_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744124.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6421..7443
                     /locus_tag="LND56_002247"
     CDS             6421..7443
                     /locus_tag="LND56_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6744125.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7460..8389
                     /locus_tag="LND56_002248"
     CDS             7460..8389
                     /locus_tag="LND56_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6744126.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8634..8750
                     /locus_tag="LND56_002249"
     CDS             8634..8750
                     /locus_tag="LND56_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6744127.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2979 a         1479 c         1759 g         2589 t
ORIGIN      
        1 taaattatat agtaggatac aacaattaat tgaaaaagaa attcaaccta ctgaacttgc
       61 tgatatagtg aagacaacga taaggtcatc aaatggaatg attagctctc ttaatgaagc
      121 aagaaatagg cattcacttg cacaccctaa tacaaatatt attggaaaga gagaagcaaa
      181 actaataata ggtatttcaa gtacagttac agattatatt agcggatacc ttgataagta
      241 gcgtcattaa ctgattgcta ctttttttat gactattttt ataaaaagga tgtgataaga
      301 attggaaatg aaagtctatg tggcaaattt aggacggtat aatgaaggtg aattagtcgg
      361 agcatggttt actccaccaa ttgatgagga agaaatggca gaaagaatcg gtttaaatga
      421 ggactacgaa gaatatgcaa tacatgattt tgagcttcct tttgatgtgg acgaatacac
      481 gccgattagt gaaatcaacc gtctatgtga agccattcaa gaaatcgaag gtacacctat
      541 ttataatgaa ctcaaagaaa ttcaaggcat gtggtttagt agcttggaag aattactgga
      601 aaacaaagag gatattcatt gttatagtga ttgtgattcg atggaagatg tcgcccgtta
      661 ttacgtggaa gaaacaggac agcttggtga agtcccttcc aatttacaaa attatattga
      721 ctaccaatct ttgggacgtg atatggaaat agagggtaat tacctagtta cctctcatgg
      781 ggtatttgaa tattgtcagt aagtttaaca atccgtgtta taattaaatt aataaatagc
      841 acggagaggt ataaattatg gtaaatgtat ttgaaaatct taaactatta aaactagaca
      901 tgaaagataa aggctgggtg atagattcct tttactttcg ttacaaacag caaaattata
      961 ttgtacttgt taagctattt gaaaaagagg aaaaagttcc agaatatgct ctgttaaaac
     1021 tagaattttt aaaggaaaac gatttttcag atatgctagc tgtctatgct aattcggtaa
     1081 aattatatac agacacgaaa acaataagag aatattttgg tatcgaatac agtagtaact
     1141 tgggagatgt tttatttcaa tttagccaaa ctcttgctcg atttattccg acagaagtta
     1201 gtgagaagaa aaatgaagac caaaaagaag ccatgtgttt ctcattaagt caaagtgatt
     1261 cagaagatcc cagaaaaaaa tattgttttt cagttagaag aaatccttta aagggaaatg
     1321 gtgagctggg aaaacgaagt ccttttaatg acaataaaac gagattgtat agacccagct
     1381 tatatgaacg attagggtca gataccaatt taagtttcag atattcaatg aatccagatg
     1441 atgaagaaac tgatgaaggt attattgcaa aatggacaaa aaataaaatt gaatagttaa
     1501 agcattgaaa gagcaatcac ctattttgat tgttcttttt ctatttttac ggaaaggacg
     1561 tgaagacatg aaaaaaataa gaagttacac cagtatttgg tcagtagaaa aagtgattta
     1621 tgcgattaat gatttacaac tccctttccc aattaccttt acgcaaatga catggtttat
     1681 cgtttctttg tttgtcgtca tactctttgg cagtttacca ccactttctc tgattgacgg
     1741 agcactactc aaatacttag ggattcccat tgttctcact gggttcatgt gccaaaagac
     1801 ctttgacgga aagaaaccct atggatttct aaagtctatc attacctatg ctctacgccc
     1861 taaagtaacc tacgctggca aacaaatacg attaaaaaaa gaaacaatga atgaatcgat
     1921 tacgattgta aggagtgaac aactagatgg catatccgat taaatatatg gaagataatc
     1981 tcgtatttaa tcatgatggc gaagtattcg cttattatga attaattccc tataattaca
     2041 gttttttaag ttccgatgaa aaaattcaag ttcatgataa tttctgtcaa ttgattgccc
     2101 aaaatcgtga tggcaagatt catgctctgc aactagcaac cgaatcaagt attcgagcca
     2161 cacaggaaca atcgaaaaaa gaaatcactg gacgattaaa agagatagct tacgaaaaaa
     2221 tagatgaaca gaccgaagcg ttaatttcta tgattggtga caatcaagta gactatcgct
     2281 tttttattgg ttttaaactc tacctcaatg aacaggaagt atccgtaaaa aatgttggga
     2341 aagaaattca aaaagcattt gcggaattct ttcatggtgt gaatcacaaa ttaatgggcg
     2401 attttgtttc tatgcctatg gcggaaatcg aacgcttttc taagatggaa cagttgttag
     2461 aaagtaaatt atccagacgc tttcagattc gtaaattgga gaaagatgat tttggttata
     2521 ttattgaaca tctttacggg caaacaggtg tcccttttga tgactacgac taccatttac
     2581 cgaaacgata cgaagataaa aaagtgttgg taaagaaata cgatattctc aaacccactc
     2641 gctgtttaat cgaagaaaat caacgctatt tacggattga acaggaagat accactactt
     2701 atgtggcgta ttttacaatt aatagtattg ttggtgaatt ggatttccct aatagtgaaa
     2761 ttttttatta ccagcaacag cagtttactt tccccgttga taccagtatg aatgttgaaa
     2821 ttgtcgccaa taaaaaagcc ttatctaccg ttcgcaataa aaagaaagaa ctgaaagatt
     2881 tagatagcca cgcatgggaa agcgacaacg aaaccagtac taatgtggtg gacgctttag
     2941 aatctgtcga tgaattagaa agtaatttag accaaaccaa agaatctatg tacaaactaa
     3001 gctatgtggt acgggtatca gcaaagaatt tggacgagct gaaacaacgt tgtgatgaag
     3061 taaaagattt ttatgatgat ctgtctatca aattagtacg accttttggc gatatgctag
     3121 gcttgcataa tgaatttatt ccctcaagca agcgatatat caatgactat attcaatatg
     3181 tcacgtcgga ttttttggct ggtcttggtt tcggtgctac acaaatgatt ggtgaaaatt
     3241 ctggtattta cgtcggttac aatctcgata caggcagaaa tatttatctc caacctagcc
     3301 ttgcaagtca aggaattaaa ggctctgtaa caaatgcatt agcgtccgct tttattggtt
     3361 cattaggtgg cggaaaatca ttctctaaca atatgctggt ctattattca gtactctttg
     3421 gcggacaggc aaccattgtt gacccaaaag ctgaacgtgg tgaatggaaa gaaaccttac
     3481 ctgatattgc ccatgaaatc aatattgtta atctcacgag tgagaacgac aataaagggt
     3541 tacttgaccc ttatgtgatt ttaaaactcc cgaaagattc agaaagcctt gccattgata
     3601 ttctcacgtt cttaacaggt atttccagcc gagatagcga gaagtttcct gtcctgcgta
     3661 aagctattcg gagtgtaact caaagcaaac aacgagggtt gttattggtg attgatgaat
     3721 tgcgaaaaga agatacgccc atcagcaata gtattgctga ccatatcgaa agctttgtcg
     3781 attatgattt tgcccatctg ttattcagtg atggcagtgt cacccaatcc attagcttgg
     3841 aaaaacaact caatattatt caagtggcag atttggtctt acccgattcc aatagctctt
     3901 ttgaagaata cactaccatg gaattattaa gtgtggcaat gctgattgtc attagtacct
     3961 ttgctttaga ctttatccac tctgaccgtt ccatttttaa aattgttgac cttgatgagg
     4021 cttggagttt tttacaagtg gcacaaggaa aagctctatc tatgcgattg gttcgagcag
     4081 gacgtgccat gaatgcaggg gtctatttcg ttactcaaaa cgcggacgat ttactagatg
     4141 aaaagatgaa gaataatatt ggcttgaaat ttgcctttcg ttccactgac ctagtggaaa
     4201 tcaaaaagac cttagaattt tttggtgtgg acaaggaaga tgaaaacaat cagaaacgct
     4261 tacgtgaatt agaaaatgga caatgtttag tttcagacat ctatggtcgt gtaggggtta
     4321 tgcagtttca cccgattttt gaagagttgc ttcatgcctt tgataccaga ccgcctgtgc
     4381 gaaatgaggt ggaagaatga cacaaaaaca aaagaaattt cttcactata gttggataac
     4441 gctcctttgt gtgggcgtta ttttactttt attaggcacg ctgggcaatg cagtacaagc
     4501 gacaggctta gttgatgaaa ccattgatac aagcaatgaa tactccaagt atggtttgaa
     4561 tcaatatcag cttgattatt atgtggacaa tagctggggc tggcttccat ggaattggag
     4621 tgatggcata ggacaatcgg tgatgtatgg attgtatgcc attactaatt ttatttggac
     4681 aatttctctt tatctatcga acgccacagg ttatttggtg aaagaagcct atgccttaga
     4741 tttcattagt caaacatccc aagcaattgg cgaaaacatg cagacgattg caggcattac
     4801 accgaatggc ttttccagtt cagggtttta tgtcggtttc ctccttcttt ttattctcat
     4861 tgttggaatc tatgttgcct atacaggatt ggttaagcga gaaacgacga aagtgatacg
     4921 agctgtttta aactttgtcg tggtgtttat tctatcggct tcctttattg cgtatgcacc
     4981 cgattatatt gcgaaaatca atgatttttc caaagatgtc agtaccgcca gtctgaatat
     5041 tgggacaaaa attgttcttc ctaacacgga tagtcaaggg aaagacagtg tggacatgat
     5101 acgtgacagc ttattctctg ttcaagtaaa gcaaccatgg ctattgcttc aatatggctc
     5161 cactgatgta gaagcgttag gggaagaacg tgtggaaagt ttattgtcta ccagtccaga
     5221 tacaaacaat ggtgaagata gagaaaatgt agtgattgaa gaaattgaag atcatgacaa
     5281 tacctattta accctaccga agacgatttc cagattagga acagtcttct ttttgtttgt
     5341 attcaatatt ggtatctcga tttttgtttt ccttctaaca ggaatcatga ttttttcgca
     5401 agtgctattt attattttcg ctatgttttt gccaatcagt ttcctattaa gtatgttgcc
     5461 gagttttgat ggcatgggca ggcatgccat taccaagtta tttaatgtga ttatgacaag
     5521 ggctggtatt accttgatta ttaccgttgc ctttagtatt tccactatgc tttattcgct
     5581 gtcagcaagt tctccctttt tcatgattat gtttttacaa attgtgacct ttgcagggat
     5641 ttattttaaa ctgggcgatt taatgagcct attcacttta caaagcaacg actctcaaaa
     5701 catgggcaga caagtgtttc gcagacctcg tcaaatgatg aatcgacaaa ccagaagatt
     5761 tcaacgcagt atgagtaaga tttttcgtgg aaaagcaggg tcaacgaagc aaaagacaat
     5821 gacaacaaag cagaatcaaa ccaataaagc taaccatact cgtcaaaatg aacggactac
     5881 accaaagaaa gaaacagcac tttccaatgt gaaaaagcaa caaggcggta tcgggcaacg
     5941 attaggtgaa aagacagccc atgtattgga cacgaaaaat cgtctagtca ataaaagaaa
     6001 gcaggcaaaa caacagatac aggcgacacc aacccatgtc aaatatgccc ttcatcaagg
     6061 aaaagagaat gtcactcata atgtaacaga ctttaaacaa tctatggcag ataccaaagc
     6121 tcaacgcaaa caacagcgta tgaaccagca ggagcaaaaa cgaaagaaca ttgctcaaaa
     6181 gcgtttggag ctggataaag caaaagaaaa aagacgaaaa gaaaacccat catctcctgt
     6241 tcagaataac cagcagcatg ttcgcaagcg tcagcaaacc gtcacgcctg ttttgtcaca
     6301 aaacactaaa cgtgaaaatc atcagcgacc attgaaaaaa gagcaaaaac aaaagacacc
     6361 accgaagcaa ttcaatcaga ggaagctaaa taaaagaacg gataagcgag gacaacaaac
     6421 atgaagctaa aaataacagt gatttgtagt gtggtcttct ctctcttctt ttttctctta
     6481 ttattcctag tcattttctt tgcagatgat acggacagtg gcgaaaacaa caaggattct
     6541 tctattccac aaggcggtgt gaccgtttca ccagaagtgt tggcacaccg tccgttgatt
     6601 gaaaaatatg gtaaggaata tgggattgaa gactatgttt cttatatctt agccattatg
     6661 caagtggaat cgggcggtac agcagaagat gttatgcaaa gttccgaatc tttgggttta
     6721 ccgcccaaca gtttgagtac cgaagagtcc atcaaacaag gggttaaata tttcagtgaa
     6781 ttattaacca gtgcagaaca acaaggagtg gatatagatt ctgttatcca atcttataat
     6841 tatggcggtg gctttttaaa ttatgtgagg agtcatggga aaaaatatac ctatgaatta
     6901 gccgaacagt tttctaaaga gaagtcgggt ggtcaaaaag cagactatcc caatcctata
     6961 gccatacctg tgaatggtgg ttggcgatat aactatggaa atcagtttta tgttcagttg
     7021 gtttcacaat acctaaccga cacttcccca acagaatttg atgatgaaac cgtgcaagtc
     7081 attatggacg aagccttgaa atacgaaggg tttccctatg tctttggagg ggcgtcgcct
     7141 accacctctt ttgattgtag tggcttaata caatgggtgt atgacaaagc tggcatttct
     7201 ctccctcgtg tcgcccaaga tcagtatgac gctacgcaag aaatctctat ggaagaagcc
     7261 caagcaggcg accttatctt ctttcattca acctataatg caggcacata tgtgactcat
     7321 gtggcgatat atttagaggg aaatcgcttt tatcatgcag gcgacccaat cggttatggc
     7381 gacttaagca gtcgttattg gcaagaccat ctgattgggg ctagacgtgt tattcataac
     7441 taagaaagga atgaatcaaa tgattataaa aatcgagcga aaagaaaaga aggagaaacc
     7501 aaagaaatct aaaaaaccga gcaagcaaag aaaagttccg atgattaaag ttggcacgca
     7561 taagaaatta acctttgttt tatgggtttt attgattggt agtgtcggat ttggaatcta
     7621 taaaaatttt actgccattg acactcatac tgtccgtgaa acagaaatca ttaaacagca
     7681 gattgtcgat accaaccaag tagaaagttt tgtgaaatcc tttgcgaaag actacttttc
     7741 gtggcaacaa tcccaagaag ccattgataa acgaaatgaa aaattgaccc attatttgac
     7801 ggaagaattg caggtattaa atgaagaaat gattcgtaag gatattccta ctagttctag
     7861 tgtgaacgat atacaggtgt ggcaggtttc tcaagtgaat gagaacacct ttgaggtact
     7921 gttttctgtt gaacaagtta ttaccgaaga caaagacaag gaaactatat cgtctagctt
     7981 tcatgtggtt gtccatatag atgaatcaga taatatggtt attatcaaaa atccgacaat
     8041 gagtaagaaa cctcaaaaat cggactacca acccaaacag ctagaaagtg accatacagt
     8101 agatacagaa acgatggacg aaatcactag ctttttagaa acgttcttcc agctctatcc
     8161 taccgccaca gaaaaagaat tgacttacta tgtaagtaat catgtgttac ctatgattaa
     8221 caaggaatat gttttcgagg aattggtgaa tcctatcttt accagaaaag ataaccaagt
     8281 aatcgtgaat gtcgctgtga aatatttaga tcaagaaaca aaggcaaccc aaatctcgca
     8341 atttgagttg atattagaaa aacaagataa ttggaagatt gtaaaataac aaatattggt
     8401 acatgattac agatactttg taatcatgta ctctttttga taaaaaattg gagattcctt
     8461 tacaaatatg ctcttacgtg ctattattta agtgactatt taaaaggagt taataaatat
     8521 gcggcaaggt attcttaaat aaactgtcaa tttgatagcg ggaacaaata attagatgtc
     8581 cttttttagg agggcttagt tttttgtacc cagtttaaga atacctttat catgtgattc
     8641 taaagtatcc agagaatatc tgtatgcttt gtatacctat ggttatgcat aaaaatccca
     8701 gtgataaaag tatttatcac tgggattttt atgccctttt gggtttttga atggaggaaa
     8761 atcacatgaa aattattaat attggagttt tagctcatgt tgatgc
//
LOCUS       DAFCLG010000107         8722 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFCLG010000107 DAFCLG010000000
VERSION     DAFCLG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.107"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            464..1618
                     /locus_tag="LND56_002250"
     CDS             464..1618
                     /locus_tag="LND56_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBK6744128.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1612..2034
                     /locus_tag="LND56_002251"
     CDS             1612..2034
                     /locus_tag="LND56_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744129.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2336..2716
                     /locus_tag="LND56_002252"
     CDS             2336..2716
                     /locus_tag="LND56_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK6744130.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2698..3612
                     /locus_tag="LND56_002253"
     CDS             2698..3612
                     /locus_tag="LND56_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6744131.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3852..4541
                     /locus_tag="LND56_002254"
     CDS             3852..4541
                     /locus_tag="LND56_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744132.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4661..4747)
                     /locus_tag="LND56_002255"
     CDS             complement(4661..4747)
                     /locus_tag="LND56_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6744133.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            5393..6325
                     /locus_tag="LND56_002256"
     CDS             5393..6325
                     /locus_tag="LND56_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6744134.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            6342..6812
                     /locus_tag="LND56_002257"
     CDS             6342..6812
                     /locus_tag="LND56_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6744135.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            6896..7200
                     /locus_tag="LND56_002258"
                     /pseudo
     CDS             6896..7200
                     /locus_tag="LND56_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7475..8656
                     /locus_tag="LND56_002259"
     CDS             7475..8656
                     /locus_tag="LND56_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6744136.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
BASE COUNT         3248 a         1400 c         1438 g         2636 t
ORIGIN      
        1 aattcaaatt attacaacag tcctaacgaa gaattgtttg taaaacaaac ggataaataa
       61 taaaaaagac tgctctttcg agcagccagt taaaaaataa tgagtgttta ttcttgaaaa
      121 agcacgcgct tatgaagcgc taccaaaaat ttaccatatt ttacaaaaaa taacaagtct
      181 taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc gaaaaatcaa
      241 gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc gtctctatcc
      301 attggtagag gggctacttg gcatagtgta cattatgtta actaacgcta taaataacga
      361 ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg catattgtat
      421 attttaatta taggtgtata ccacttaaat ttaacacaaa ggcgtgatta ttttgaaacc
      481 caaaaagaaa atttatctag gtctcctctt ttccttgatg attggaattt ttagtatagg
      541 aattacaaat acctatgctt cagaaaattc taatttcaat atcaactccc aaccaattga
      601 agaacaagca attaattttg cgaaaactga ttttgaacac tccataactt caattctttt
      661 aatggaaaat aacaccgaaa ctgatattaa ctatactctt ggttcgccat ttaaaatgct
      721 cgatggagaa aatacggcaa atagttctat tttctttcca gtattttcag acgctgataa
      781 tacgattaaa tatatttata ccgtcaaaaa aaatacaaat ggagaatatt caggaagtat
      841 ttcacaattt ttagcaaaag aacttcaaaa tctagtagat agtaatataa cagatacaac
      901 cttatttttt caagataata gcatttatta ttccgataat ggacaaatta aactactttg
      961 gaaatcacct ttaagtacag aaaaaaatgg tgtttcttcc tttacagaaa aaaatgatct
     1021 tatatccgta aatataaaaa ataaaattaa aaatttctct aaaaaacaaa attttcctgc
     1081 gattaaacgt gtaaaaagtg tctcctccgg agataacttt attatgatta attggcgtat
     1141 agcagaaacc caaacaagtt ttccgtggtg tgctgcttac gtagaagctg ctatcttatg
     1201 taacaaaaca gatgtacatg ctacttcagc tagagccatt atgcattata cctatcctaa
     1261 tttaaacaac agtcaaattg aatcaaaaag tcttaatcaa gatcaaataa taaaatatgc
     1321 aaattactgt ggtgtttatc ctaaaaaagt agcacgaata ctaaaccttt cagaggtcca
     1381 aacacaacta agaaaaggaa atgccattta tatgggaact gctggttatg gaaattatag
     1441 taacagccgt catgcacttg cattatttgg gtgggtcaat caaggtggtt atcaaactta
     1501 ctatgtgtgg aatccttggt ataactaccc aactgtaata agtgcaaatg caggaccagt
     1561 tacaattcct gtacctggag gtggttttac ttgggacaat accattacaa attggtaaat
     1621 aaacccattg ctttagttat gggtctagtg ttagttcttt tcttaactag ttgttcaaaa
     1681 aaacaagaaa tacaaaagac tgatctgatt ccttttctag gaacttggca aactaaagat
     1741 cattcagaac gtctagtaat cactaattta aatacttcaa aaaatataga acaatatacc
     1801 atcttagtaa aagagaacaa tcataaaatt caaatcattg ataaaaattt aaatataaat
     1861 gaacaagttt tcatttacaa agcaacaaat aatttactaa cactgcgctc taataaaaaa
     1921 caattagatt acacttttag tttggattct ccaaaaaagc tcgtatttat tttgacaacg
     1981 aatgatggga cagaagggac agctaaacca attgaatttt ttaaagctga ttgatgaata
     2041 gttgtttgat attacaaaac aataatttca ccgaaggtga aacaagcgaa actttacaaa
     2101 aagtggctag catttgctag ccttttttgt attgtggagc gtgggttttg agggggattc
     2161 aagggggagc ttgcgcccct ttgaaaagcg gttatttgca ccaaatacgt aggtatttgg
     2221 ctagcaaata tagagcttgc caaacccttt tagtgagctt tactcattgt acctttttag
     2281 gtacaatggt attgcgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag
     2341 cgaacaaaat caaaatttgg ctagcgatca ttctaaaaaa tatacctatc gatccgagcc
     2401 aaaacaaatc agttttcgag tgagcgaatc tgaatttgca aagctgaagc agtcagctga
     2461 agctttgcaa atgagtgtgc cggctttcgt aaaagccaag gcacaaaacg cgcgtctggt
     2521 gacaccgaaa gtggcgccag acattgccca ggcaatcgcg cgcgatttag ccaaagctgg
     2581 agggaatatc aatcaaattg ctaagtggtg taatacacac caacacgacg ttgctcctgg
     2641 agacgcccaa cgcttatccg aaaacctaaa aatcatgcaa aaggagctac aaaagatatg
     2701 gcaacagtta aagtaagccg aaccacttct tgtagtcgtg caattaatta cgctgaacca
     2761 cgagcaacca ttaaaacagg aataaactgt gacgtcgatt atgccaaaag tgaaatgaaa
     2821 caaattcgca tgctgtatgg aaaagacgac cgtgtacagg cacacttatt gattcaatct
     2881 tttcgaccag gagaaattac cgctgaaaaa gctaatcaac taggggcaga gtatgcaaaa
     2941 aaaatagccc ccgatcacca aatagctatt tacacacata cagacaaaga ccatattcat
     3001 aatcacattg tcattaactc cgttaatctg gagactggaa aaaaatttca agctcatggt
     3061 caagcatttc taaacaaatg ttatgaccta aatgacgaga tttgtcttgc acataactta
     3121 ccaatcactg agcgaggaaa gaaaccagaa aaacgaacca tgtctgaaat aaaattaaaa
     3181 gaaaaaaatg aacctgtttg gaaagacgaa attcggattg cgatcgatca aacaatgaaa
     3241 aacaaaaaaa ccagaaccta tgaccaattt tgtaattccc tcaaaacatt ggggattcat
     3301 tgcttcaatc gcgggaaaaa tttcacttac gaattgatcc acaaaaagaa aaaagtacga
     3361 tccaacagac taggaaagga ctacgaaaag gagacgattt tacatgagct ggacagacga
     3421 gaacgaacct ataaccaaca gcgaaccaac gagctacgaa gagctcgagc tagcattcgc
     3481 gaaactgcag aagaagtacg aagagaacaa cgaactctta cgcctgaaca accggaaatt
     3541 agaccggctc gaagaaacca tgaacaacac gttgaaagag gaacagacca ccaacaagaa
     3601 ttatcgctct aaaacgttaa aagacataga aaacgaaata aagaaacaac gtgcttttct
     3661 cgttccttat acgcaagaaa ggatacaaac tacgattgat ttacaagtta aaaaactcca
     3721 tcaagagcta ttagaccagc gaaataagga acagaaaaag gtgcttattc aacgatcatt
     3781 ggattttctt gcccccttat gttgcttagt cctgacactg ttcgcatttt ggttagtatg
     3841 gggaggctaa aatggcattt tatttaccct atctactcat ttttgttagt atttcaggaa
     3901 gtatttggct catctataaa atttttcaaa caagatattc attaaaaggc tctaaaattc
     3961 gatttaagcg atttttcttt cttggctgta tattttcctt gataatcgtt tcaagcggct
     4021 tattcggcgt tttagaggga aataagcgcg tttctagaag tattttatta ggaaatgtca
     4081 ctcaaaagta tgaatcagcg cgtgataaaa agaaaaaaga gcaagtatta gcacaaaaaa
     4141 tagaaaaatt taccgcttgt tatgaggaca tgaatgatat ttttgtgaag caagaaaagc
     4201 gtttgacgga taaaaatatg gaaacactca ctcgacttta tcggaatttg ccagaagaac
     4261 agcaaaaaga gtaccaagaa aaatatgaac aagtaaaaaa agacgtgcaa tatgtgaaag
     4321 atacgaaagt ggaagaagct tgtagtgatt tatttggaga tacaattcct ttttcaacta
     4381 gcgaacaaga acgaaaagaa cgacaacaat cagtcacata tgaacgctat gaaatcttgc
     4441 ttcaacaagc aattaacata caaaatctga ctaaaaaaga aacagcttta aattatttaa
     4501 aaagcgtcaa ggaatggtta gatcagcaac agcaaaattg aatttgattg aaattcatgt
     4561 taaactaaac atgcaaatgt tcaaacatta gatacacgaa agccccagag aggtcggatt
     4621 ctctggggct ttttttaggt tgatgtcgcc tttactgatt ttacttcttg tttcggttat
     4681 tcaaccaata actgaataca gagatacaaa ctcctacaag aagggggcaa ataaacagtt
     4741 caaacatcag atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat
     4801 accctacaaa attaaatctg tgtggcaatt ttaagtatct cttatttccg ggttgttagg
     4861 gcttgccctg accgcctgta gggcgcttta aatgccagat aaaggttttt atactaagac
     4921 tgtcttaaaa cggcttagac aagctcttag agcctcaacc ctcctttttg aacaatggtt
     4981 ttagcaaagg agctggaggc gactgcgcag gtataatgtg agagcgtagc gatcacacct
     5041 ttttggcgac cgtagggagc gaggttttga attagcagtt tagaaaagtt cgcgaacctg
     5101 tttaaagaaa aaataattgt ttttttaggt gggaaatttt gtctccctct tttataatct
     5161 ttttttaaac ctctttttaa acctctttac cgtcggctgt gtccttactc ccccaaggga
     5221 tacaaaattt tataggcatg aaattatctg tttataggca tgaaattatc tgtttatagg
     5281 catgaaatta tctgtttata ggcatgaaat tatctgtttt aggtattgtt tttagacata
     5341 atatgtccta aaatgatgtc aataaaatca tttagactgg agtaacaact aaatggcaaa
     5401 tgaaatcgta aagtatgaac cagaattaaa taccattcct ttaagaaaat ttaccccaat
     5461 agaaatgaac ttgttttttt ctattgtctc tagaatgcgc gataaaggta atcaaaaaat
     5521 acgcttttat ttcagccaac ttaaagattt aagtgcgtat aagcagactg caggagaagt
     5581 ctttataaaa gatttggcaa gaacatacga tcatttgatg gatttacgtt tcggaagaaa
     5641 aagcaatggt ggattaacaa tagaacgatt tacaatgttt actgattttg aaattcatgg
     5701 agaagtcgat aacccttatg tagatataaa agtccacgaa aaagctttgc ctttacttaa
     5761 taatctagaa agctgggtac gtttctcttt aacagaattt cgagatttaa atagcagtta
     5821 tgctaaaaca atgtttagat tgctaaaaca ataccgaact caaggatggg ctgaattttc
     5881 taaggaagat tttttagagc tattagatat tcctaaaagc tatagacaaa caaatataaa
     5941 tcagtttgta ctcaaaccaa taaaagaaga actaactcca ctatttaaag gattaactat
     6001 tcgtaaaaaa tatggtaaag gtcgaggtaa acctgttatt ggttatcgtt ttacttggaa
     6061 agctgaaatc aaccatgctg acgatttttc aaaagggaag caagaagatt tacgtataaa
     6121 attatttaat attgaacaca atggagaatt aacacaaaaa gaaaaatggc gtgctaaaga
     6181 tcgaatacta aatcttccgt taggtactca tgaagctgat tttaaaaaac aacaacaaac
     6241 cgaaaaagaa gaagcggaaa aacaatcaat ctcaaatgag cttaaacaag atttattaga
     6301 aaacttacaa aacttattcg attgaagcat aaggagtatt tatgatagat aaaacgatca
     6361 aagaactcgc ggaagaattt catgtttcaa aacaagcaat taggaaaagg ctatcagctg
     6421 attttagaga aaactatgtg caaactgtta ctaggaacgg agtgaaaacc ttagttgtca
     6481 gctacaaagg atatttgctt ttaaaacaac attttttagg tagtaacacc actggtaacc
     6541 aagtagaaac cgttgctagt aacgatgaaa actcgattat tgttttttta catgaacagc
     6601 tccaagaaaa agatactcaa ataaaacaaa tgcaaaagtt actcgaccag caacaacaac
     6661 tcaccttgca ggcaaatcaa cagatcgaaa aactgcaaga acagttacaa ttagcttata
     6721 cggaagaacc tgaagaagag gaattaactt ctgtcaataa agaaaacaac aatgcaataa
     6781 caacagaaaa aaagtggtgg aaattttggt aacaattacc aactttccat aaagaagaaa
     6841 tcaatttcta tagactctat ctttctaaga ataaaaaata tgtttaggtg atcaaatgac
     6901 tataagtaaa gtaatcaaac aaaaaagaat agaaaaacaa ctaactcaag aagatttagc
     6961 tgaaatgctt ttagtatcta aaaaaaccat ttctaattgg gaaaatggtc gaacaatacc
     7021 agatacagaa aatttaattc agtttgctaa gttatttgat ttatcactag acaatatttt
     7081 gttaggagaa gaaaaaatta tggtaaaaaa tcatacaaaa acactaatac tattatttca
     7141 ttttgtaatt tttttaagtt tagttttcac cttaattcag tccggattct tttattttag
     7201 ccattcaatt gccacttggt tattaatatt ttttctaagt ttactattaa taattaatac
     7261 tttaattatt atctttaata aacatagaaa cttatttctt cacagcatat ttccaattgt
     7321 tttattaatt tttctatact ttatataaaa agatagaact atttagtaag ataaaaaagt
     7381 agctaattgt aattttcgtc taaaactcaa aactagtaaa aataacttta ttgtgttatt
     7441 tttatttata aattatgaaa aaggaccttt cgaaatgaaa aataatagaa tctacaaaaa
     7501 tcagttgaat tatgggcttt tattgccagt ttttctgcta tgtatcattg gattactttc
     7561 tttatatgtt gctttatatc acgactctta cactaattca aaaatcacaa aagaattaat
     7621 taaacaagta ttgtggtata gtattgggtt cacttcagca ataactattt atttttttaa
     7681 tcctaaaatt ttgtggaaat taactcctat attttacgga ttatgcatca taatgttatt
     7741 acttctcttg aaattctata atccacaatt agcagtctta actggagcaa aaaattggtt
     7801 ttatattgct ggtttttcgt ttcaacctac tgagttagtc aaaataggat acacccttat
     7861 gctagctaaa ataaccgtca attatcagca aaaatctatt actagtaata cgaaagatga
     7921 ttggatttat ttacttaaaa cttttattgc tactcttcca ataggaatat tattattttt
     7981 tcagaacgat ctaggaacaa tgttagtctt cattgttatt tatattttta ttttgtttac
     8041 gtctaacata aatataaaaa taattgcccc aataatcgcc attggtgttg ttttatcctt
     8101 aatattttta tatttggtta tatcagatac aggaagagat ttcttattaa aaataggatt
     8161 tcacgactat cagtttaaaa gaatagactc atggttgaat ccatttttag atccaaatgg
     8221 atctagctat cagttagcaa atagtttaat tgctattggc tctggcggac ttttaggtac
     8281 tggttttaat gtatcaaaca ttcacgttcc tgttagagaa tccgatatga tattttctgt
     8341 aattggagaa aattttggct ttataggaag ttgtcttgta atattcctat acttttattt
     8401 aatttatcaa atgtataaaa catgttttaa aagcaataac ttatttttca cctatatcgg
     8461 tataggcatt gttgcaatga ttttttttca tgtttttgaa aatataggtg caagtatcgg
     8521 attactacct ctaactggta ttccactccc ctatattagt caaggtggtt ctgccattct
     8581 tagtaatttt ttggggttgg gatttatgtt gtgttgtagt agatatacta acgaaaaaaa
     8641 aggaacacaa aaatgaattc aaaataacac tttatgaata gaatgtctat ttcaataaaa
     8701 attatgatac cttaaagtta at
//
LOCUS       DAFCLG010000108         8659 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFCLG010000108 DAFCLG010000000
VERSION     DAFCLG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.108"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="LND56_002260"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="LND56_002260"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK6744137.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="LND56_002261"
     CDS             complement(1098..2201)
                     /locus_tag="LND56_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK6744138.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="LND56_002262"
     CDS             complement(2291..3247)
                     /locus_tag="LND56_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBK6744139.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="LND56_002263"
     CDS             3528..5462
                     /locus_tag="LND56_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6744140.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="LND56_002264"
     CDS             5919..6335
                     /locus_tag="LND56_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK6744141.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="LND56_002265"
     CDS             6349..6915
                     /locus_tag="LND56_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBK6744142.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="LND56_002266"
     CDS             6938..7924
                     /locus_tag="LND56_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6744143.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8659)
                     /gene="scm"
                     /locus_tag="LND56_002267"
     CDS             complement(8079..>8659)
                     /gene="scm"
                     /locus_tag="LND56_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6744144.1"
                     /translation="SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDS
                     TDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDS
                     SDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKT
                     GSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2537 a         1669 c         1767 g         2686 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgtgtccgtg ctatcacttg tggtagaggt agtatctgta
     8641 ctgtcgcttg aagctgacg
//
LOCUS       DAFCLG010000109         8365 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFCLG010000109 DAFCLG010000000
VERSION     DAFCLG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.109"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..1641
                     /locus_tag="LND56_002268"
     CDS             118..1641
                     /locus_tag="LND56_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK6744145.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            1878..3218
                     /locus_tag="LND56_002269"
     CDS             1878..3218
                     /locus_tag="LND56_002269"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBK6744146.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3371..4108
                     /locus_tag="LND56_002270"
     CDS             3371..4108
                     /locus_tag="LND56_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBK6744147.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4273..4494)
                     /locus_tag="LND56_002271"
     CDS             complement(4273..4494)
                     /locus_tag="LND56_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBK6744148.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4525..5556
                     /locus_tag="LND56_002272"
     CDS             4525..5556
                     /locus_tag="LND56_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744149.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5543..6946
                     /locus_tag="LND56_002273"
     CDS             5543..6946
                     /locus_tag="LND56_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK6744150.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            7012..7647
                     /locus_tag="LND56_002274"
     CDS             7012..7647
                     /locus_tag="LND56_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6744151.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7696..>8365
                     /locus_tag="LND56_002275"
     CDS             7696..>8365
                     /locus_tag="LND56_002275"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK6744152.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGY"
BASE COUNT         2748 a         1431 c         1793 g         2393 t
ORIGIN      
        1 tttttgaaca aaagttttac aaaatgcgtg ttattatttt tgttctgata ctctttttga
       61 taaaataaca ttagagccaa aattgttgtc tctaacggaa aagaaaggaa gaagaagatg
      121 gaacaaacaa aaaagaaaaa atttcaaatg ccttcagcgt atacggtctt atttattatt
      181 attgcagtaa tcgctgtact tacttggttt ataccagctg ggcactatag tgtagacgat
      241 gcaggcaaca tcgtcgctgg atcatatgaa cgagtacaac aaaatcctca agggctgtgg
      301 gatattttca tggcgccaat caatgggatg ctaggtgtac cagcggggga gctaactaaa
      361 gaaacacctg ctgcaatccc aatctctttc ttcattttgg ttgttggtgg atttttaggt
      421 attatcaaca aaacaggggc actagatgcc gggattgctt cagttgtgaa gaaattcaaa
      481 ggaaaagaaa aaatgcttat tccagtattg atgctgttat ttgcattagg cggctctact
      541 tacggaatgg cagaagaaac gattccattc tatgctttac tgatcccagt catgatggct
      601 gtaggctttg atacagtagt agctgtagca atcgtattag tagggtctca agtaggctgt
      661 ctagcttcaa cggtaaatcc ttttgcaaca ggcgtagctt cacaagctgt tgggatttcg
      721 atgggtgaag gtattggtct tcgattcttg atgttcgtta ttttgttagc tatttctatt
      781 gtatatgtgt atcgttatgc atcaaagatc gaaaaagatc caacaaaatc attagtatac
      841 aaccaacgta aagaagatat gaagcatttc gatattgaag caatcggtcg tcaagaagta
      901 atcacgaaaa aacaaaaaca tgttaatgta ttgttcttta ttacttttgg tatcatgatc
      961 atcagtttga ttccttggac aaccttgaat gcgaatttca cgatttttga atcattgaca
     1021 aactggttaa caaaccttcc ggtactaggt acggttctag gtaaaagcat gcttccatta
     1081 ggtgactggt acttcccaga aattactatg ttattcttag taatgtccat cgtagtagca
     1141 atcacttatg gcatgaaaga gtctgaattt gtctctgaat ttttagctgg tgcttctgat
     1201 ttgatcggtg tagcaatcgt agtagctgtt gctcgtggta tccaagtggt tatgaacaat
     1261 ggattaatca cagacaccat cttacattgg ggcgaacaag gattaagttc attatcttca
     1321 agtgtattca tcattattac atttattttc tatatcccta tgtcattctt gattccttca
     1381 acttccggct tagctgctgc aacaatggga atcatgggac ctatgggtga atttgccggc
     1441 gtcggtaagg atttagtaat cactgcttat caatctgcat ctggtttagt aaacttaatt
     1501 acaccaacat ctgctatcgt aatgggtgct gtagcaattg ctcgtttcga tgtttcagtg
     1561 tggtggaaat ttaccggtaa attaattgcg atattatttg tagccatctg tgtgatccta
     1621 ggtgtagcag caatgttcta aacctagaca aatacaggta caaggtctat cataagaatg
     1681 aagaaaccga taaacggcta tcgataagca cgtggataag tcaatagctc cattcagtga
     1741 gtgttttttc tttcatcatt tgttaagaac attctgcttg ctgatggagc aggcttaaaa
     1801 cgtcattatt tgagaagaag aggttgtgtc ggaggaattt gacatgaccc tttttaacat
     1861 attaggagga taatatcatg aaaaaatttg tgacagaaca acatcatgaa caagcattag
     1921 aatcattgag cgaattgatc cgaattcctt ccgtactaga tgaagcagat tcaggacaag
     1981 gacatccctt cggtaaaaaa gtgattgaag ctttggataa agtattggaa atcagtgaaa
     2041 agctaggatt tagaacattt aaagatccag aaggctatta cggttattca gagattggct
     2101 ctggagatga attattcggt atcttgtgtc atatggacgt tgttccagca ggggacgaaa
     2161 ataattggga aacaaaacca ttcgatccaa ctataaaaga cggctggtta gttggtcgcg
     2221 gatcccaaga tgacaaaggt ccttctattg cggcaatgta tgccgtcaaa gctttgattg
     2281 atgctggcgt ggaattcaag acacgtattc gttttatctt tggtacagat gaagaaaatt
     2341 tatggcgttg tttagaaaaa tataacgaaa aagaagaagg aattacgcaa ggatttgcgc
     2401 cagatgctga atttccttta atttatgcag aaaaaggctt attgcaagct tacttgacag
     2461 gaccaggaac gaacgagttt tctgtaaaag ctggaggagc gttgaatgtt gttcctgatg
     2521 cagctcctta ctctggagaa aagctttctg aagtaaaaga agcattgaaa aaacacgaat
     2581 tcgatttcga agaacaaggt gaaggcatcg tcgttcttgg taaaagtatc cacgcgaaag
     2641 atgcagcaca aggagtgaat gctatttctc gtctggcaat tgctctttca gaagtatttg
     2701 attttggccc aatcaatttc ttaggaaaat tagttcaaga aaatgcaact ggtgaagctg
     2761 tcgtgggcaa aacagaagat gaacaatccg gcgagctaac gatgaacttt gcaagtttgg
     2821 aaatcacacc agaacaaaca aaaatcggtg tagatatgag aatccctgtt acattcaaga
     2881 aagatgattt agtagctaaa ttaactaaaa cagcagaaaa atatgggttg acttatgaag
     2941 aatttgactt ccttgattca ttatacgtgc cactagacag tgagttagtg aaaaatctat
     3001 tggggacata tcgcgatatt actggagaca tgactgaacc gtttgtatca ggcggcgcta
     3061 cttttgctcg tacgatgaac cagtgtgtgg cattcggtgc gatgttccct gatactcctg
     3121 actttatgca ccaggcgaat gaacgatggg aattaagtag tatgtataaa gcaatggaaa
     3181 tttatgcaga agctgtgtat cgtttatgtg ctaaataaaa ataaacatac aaaaagggcg
     3241 gatattcaaa tggatattcc gtcttttttt atttgatgct ggaaagtttg catttctctc
     3301 ttattttttt aaggtataat catacttgat taagaaaaag gggtcactca gatagaaaag
     3361 gtgggagaaa gtgaaaatat ttagacgaat actaattggc ttagtcatca ttttaggatt
     3421 actcggtgga gccggttatt actatataca gcaaaatacg tacgaaccat cgtcagaagc
     3481 gcaagtgttg gtaaaagaag gaaaaaacaa agaagattac ttattttttg aaggagagaa
     3541 aaagaatcat ggaaagtctc cattacttat tttttatcct ggtgctctcg tagagccaga
     3601 aagttatagt atttgggcgc aagatttagc aaaaaacggc ttttcagtcg ctattgtcaa
     3661 aatgccactt gatctagcag tgatgggcgg aaacaaagcg gataaggtca aagaagattt
     3721 tccaaattcc gattatgtga tcggtggcca ttcattaggt ggcgtaatgg caagtcgtta
     3781 tgtcaataag aatcattcag acaaacattt aaaaggggta ttcttcatgg caagttatcc
     3841 cgacaaaaaa ggaacattga cagaatcagc tgttcctgtt ttatcgatca cggcgacaaa
     3901 agataaagta ttgaatcagg aagcttatca agaagctaag cagtatttac cggaaagaac
     3961 agagtatgta tcaatcaatg gcggaaatca tggaggtttt ggcagctatg gtgagcaaaa
     4021 aggagatgga gaggcagaaa tttctaacgc agaccagcaa actgtgatcg tcaatacatt
     4081 ggaaaactgg ctgaaaaagt tgaaataata taaatgggtc ctatagggtc cgtgaaaaaa
     4141 ctagtcaatt aactatagat agcaaatgaa tctttcaaaa taagtcggga tttcacggaa
     4201 caagagaaac agatcccggc ttattttcaa gtttagagaa cttagtgtac gtttttaaag
     4261 tttcgtttat atttatattt tattttctaa taaaatcgct cgaactggac atttttgata
     4321 cgcttttttt acatatgttt cttcttccgg catcacgaac tgttgatgag cctctggttc
     4381 ttgggcaaat aaaacgattc cttcttcatc ataatcgaaa atatctggcg cttcgatttg
     4441 acaaagaccg catgcaatac agcgttcagg tacaagttta catagtagtg acatttctaa
     4501 ccatcctaac ttaaggagaa taatatgatt gtttttattt tatcgctatt ttcatcagga
     4561 cacaagctgc ggattagttc tctttaccaa ctccttgtgg gaaaacgaac gacttcagta
     4621 cttatttatg gatttaccca cgaacttttg tttatccata acagttttcc agaattgaag
     4681 caggataagt tttatcaaat tttgcaaaaa attgcgcagc aaggttggat cgaaataaat
     4741 gaaaatgaag caaagctaac gcctgcaggg gctgatatgt tgtccgaaca tcaaatagaa
     4801 catactggtt tgcgttttga ccgctatggc agaacagggg aaactagttg gcggctcatt
     4861 aaatttgctg ttcaagtaat ttctaatctg gcaacgggta ttcaagacta tcttccagca
     4921 gagacaagtc ctttttatac tttccaacta aaaaaatggc ttagtgaaag ccaattgcca
     4981 cgtgaaattt tgattgatac tgcgtatgag agtttggtgc agctgttttc ggagatacca
     5041 gaagaagcag cagatttttt agctaaccaa ttatctggaa atgagcgaac agggctgctt
     5101 ccttatcagc tggcaaaaaa caatgatgag tcagcggttt atctgcaaca aagcagatgt
     5161 atccaccttt tgttagcaca aatcgaaaaa cggccagatt cgctttggca cgctttgatc
     5221 gattcattat tacagcaaaa tttcaatcaa agcatgatga tcaccaagca gatgtttatg
     5281 aatggtcaga caattgacca gatcatggca atccgtcatt taaaaagagg aacagtaaca
     5341 gatcatctta ttgagtgggc cttgtttttt gatgattttc catacgagtg gattctctct
     5401 gcagaaacaa tcgaacgttt ggaaccaaat aaggattccg tgcgagagtg gcgctttagc
     5461 gaatggaatg tagatggaca attagattat ggagaatttc gtttgtatca aatctatcta
     5521 ttgagaaagg aagcgataca gaatgttaat aaatgaatta aaaaaatatt ttggatttga
     5581 ttcatttcgt cccggccaag aagaaattat tcaggcgttg cttgatggag aagatactct
     5641 cgctattttg cctacaggaa cgggaaaaag cctttgctat caattgacgg ggtatttaat
     5701 ggaaggtctg gtcatcatcg tgtcaccgct gctttctcta atggaagatc aagtgacgca
     5761 attacaaaaa cgaggggaga aacgagtagc tgctttcaat agtttacttt cgcaatctga
     5821 aagaagatac gtcctccgtc atctatcgca atataaattt ttgtttttga gtcccgaaat
     5881 gttgactaat ccttctcttt tagagcatct gaaaaaacaa aatattgcgt tatatgtagt
     5941 agatgaggct cattgcgtgt ctcaatgggg agttgatttc cgtccagaat atcaacagtt
     6001 aggaaaaatc cgaaagcttt taggaaaccc ggtcacactg gcactaacgg cgacagcaac
     6061 agatttagta gcaagagata tacgtcatgt actatttgat cgaccaccaa aagagatcag
     6121 acaatcagtc aatcggcaaa atatctcgct ttttgttcgg aaaacgcagc aaaaagaaca
     6181 agaacttgaa cacttcatgg aaagagcaca aggagcggca atcatttatt gtgcaacaaa
     6241 aaaagaagtc gaacgattgt atcatttatt cagggaacgt tttacagttg gatactatca
     6301 cggtggtctt gatgcagctc aaagaagaca acttcaacag cagtttgtaa aaaatcagct
     6361 gcagtttctc attgctacga atgcatttgg tatggggatc gataaatctg atatccgtta
     6421 tgtggtccat tatgatttgc cagacagtct agaaaactat gtacaagaaa tcggtcgagc
     6481 aggcagagat caggaagaaa gtgcagcaat tcttttttat caaagtgggg atgaacgtat
     6541 ccattatttc tttaatcaac tttctagaga acagcgacaa agttttgagc tttatttgga
     6601 atatgccaca gagcaagctc cctttgatga gctgcaaaaa aaatggatgg agctgatcca
     6661 gcagtctgaa aaaccagaaa attgggtgga acggctcaaa cgtcaggaaa aagaaaaaga
     6721 attccgcctg cagcagatgc ttcgctatat taatgaagaa aactgccgca gaaaattcat
     6781 cttagcttat tttggcgaaa aactctccga aaagccgcag aattgttgtg atattgatgg
     6841 agcaaaagca gaacttctgc caaagaggaa gcttttttct aagactgaac aacttcattg
     6901 ggaaacgatt ttactaaatt tatttaaaaa aaacatggaa cattgatttt ttaggacaag
     6961 acactttttc ctatggtata atacacacga tagagtttag gaggtaaaac agtgagcaga
     7021 aaagatagac atcaatctac agaaaataac gaaacacaag aaccatggga acaaccaatc
     7081 tacgatacag atgatgaagt ttcatcaaga agttctcaga gacaacaaaa gaaaggaaat
     7141 acattatttc tttcgctatt tttgatttta ctggtcttat gtatcgctat tccagcaggt
     7201 gcttatttct ggatcagaaa tggatcaagc aacaatgcaa cagcagcaac ttcttctgaa
     7261 acttcgacat cagtagtaga aagttctaca agtacaacaa aagaggagtc aactgagact
     7321 tcaacagtag aatctagtac aatcgcggaa gaatctactg tgtctcaaga tacaggtgta
     7381 acgtcagaaa gcagtattgc agcagagaca acaccgactt cctcaagtga ggcaacttat
     7441 caagcagggg attcaagcag caatgcagca gaaacaacac cttcttcatc agctgcagcc
     7501 ggttcaacaa cgactgttca agcaggcgaa ggtccaaaac aagtggctga gcgtgcaggg
     7561 atcaccactg atcaattgtt tgaattgaat ggcttagatc caaacaactt tatgctttac
     7621 cctggtcaag aattaagaat taaataataa tagccgcgct ttgcggcttt ttttctgaga
     7681 aaaaggagtt atatcatgac acaaatcagt attgcaatcg acgggcctgc ttcatctggg
     7741 aaaagtaccg ttgccaagat attagcaaaa gaattgaatt atatttatac agataccggt
     7801 gccatgtatc gcgcagtaac ttatttagct attacgaaca atgtatcttt ttcagatgaa
     7861 gaagggctag tttcgctgat caaaaagtat cctatctctt tttcgcaaca agaagatgga
     7921 cagcatgtat tcatagcatc aaaagatgtg actttagaaa tccgtcaacc agatgtcaca
     7981 aaagctgttt ctgaagtctc tgcacatgga aaagtcagag aagaattagt tgctgtccaa
     8041 cgaaaaatag gggaacgcgg tggcgttgtc atggacggaa gagacatcgg gacagctgta
     8101 ttaccgaatg cagaagtgaa aatcttttta gtagcaagcg taagtgagcg tgcagaaaga
     8161 cgtttcaaag agaaccaaga aaaaggaatt ccgactgatc ttgaaacact gacgaaagag
     8221 atacaggaaa gagaccatta tgattcaaca agggaagtct ctccgctgaa gcaagcagag
     8281 gatgctgttc gtatcgatac gactggcaaa tcgattccag aagtcgttgc agcaataaaa
     8341 gctgttgttt tgaaaaaagg ttatc
//
LOCUS       DAFCLG010000110         8319 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFCLG010000110 DAFCLG010000000
VERSION     DAFCLG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.110"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            628..2721
                     /locus_tag="LND56_002276"
     CDS             628..2721
                     /locus_tag="LND56_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6744153.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            2881..3747
                     /locus_tag="LND56_002277"
     CDS             2881..3747
                     /locus_tag="LND56_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6744154.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="LND56_002278"
     CDS             complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="LND56_002278"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK6744155.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            4865..7543
                     /gene="mgtA"
                     /locus_tag="LND56_002279"
     CDS             4865..7543
                     /gene="mgtA"
                     /locus_tag="LND56_002279"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK6744156.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKGSADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            7630..8301
                     /locus_tag="LND56_002280"
     CDS             7630..8301
                     /locus_tag="LND56_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6744157.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
BASE COUNT         2723 a         1422 c         1751 g         2423 t
ORIGIN      
        1 tatcaagagg tcttgagcct gccgtttaac tctgagccac aaggagcaat ttcaaaaaat
       61 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      121 gtttaccaag aggttgtaaa attagcgtcc tgacataatc taataccgag ggtcagctga
      181 ttgaacaccg tgtatcaaga ggtcttgagc ctgccgttta actctgagcc acaaggaaca
      241 atttcaaaaa acagcttctc atgttttata aatattgtga cttgtggccg aggagttggc
      301 aggagaacac cgtttaagga agaagttgtg aaaaagctgt cctgcatcaa gaaataagga
      361 gctgcgacaa gaattttgtc acagcctctt tttttgtacg aataccgaga aactggcttt
      421 tgaataccgt ggagtgaagc ggtcaccaca agacttttat ctcttcatta gtttaatcgc
      481 ttacatcgca gcggttttat gctgtgaaaa gtggacggaa taatctttag aaagaataaa
      541 aaaatcttaa gataacaact tttttaggaa attttcattt ttcaggagca aaataagaaa
      601 aaatgtgaat agagagaagt gatatacgtg acacaactta aaaaattgtg ccaaaatcgt
      661 ttagcaatcg ttttgacagc tgttttcttt ttctggctga agacgatcat tgcctactat
      721 gcagattttt cattaggtgt ggaaggtacg atccagtact ttatcttgtg gatcaaccca
      781 attgcgacta ccttattgtt ttttggcctt tctttgtata ttaaaaagcc aaagccagcg
      841 ttagctattc ttttgatcat tgatatttta aatacgcttt tgttgtactt gaatattatt
      901 ttttatcggg aattcacaga tttcattaca gtcaaatctg tattaggctt ttcgaaagtg
      961 tcgcaaggat tgtcaggaag ctccttttct ttgatgaagc cccatgatgt catctactgg
     1021 ctggatatcg ctgtgttcat tgggctactg gtttggttga aggtcaagaa aatacctatc
     1081 aaaagcaacc ctgtcggcaa accgatggct atcgctgtta cctgtctttc agggcttatt
     1141 ttttcaggaa atctagcgct gagcgaagcc aaccgtccac aattgctgca gcgaacattt
     1201 gaccgttcat atattgtcaa atatctagga atcgatgcgt atactattta tgacggaatc
     1261 aaaacaggaa tgactagcag tgttcgcgcg catgcaagca gtaatgggat tgatgaagtt
     1321 ttagattata caaagaaaca ttatgcagag ccaaatcccg aaacatttgg gatcgctaaa
     1381 gggaaaaatg tgatcgttct ccatttagaa agtttccagc agtttttgat taatatgaag
     1441 gtagacggac aagaagtcac accgttttta aatagtattt tccagaatca agcaacgatc
     1501 agctttgaca atttctttca tgaagtagga cagggaaaga caagtgatgc tgaaaatatg
     1561 ttggaaactg gcacatttgg tttacctcaa ggctcattat tcacagaact tgggtcagac
     1621 aatgtctttc aagccgcacc agcgatcctt ggacaaaagc aaggctatac aagcgctgtt
     1681 ttccatggga atgtagctag tttttggaat agagatcatg tatataaaaa cttaggttat
     1741 gacaatttct ttgatcgatc ttattttgat gaatccgacg aaacgttagg ttatgggata
     1801 ttagataaag atttattcag agaatctgcc caatatcttg agcatctaca acagccgttt
     1861 tacacgaaat ttctttccgt aaccaaccac acgccttatt atacggatga caagaatttc
     1921 gactttccat ctttgaatac tggcaatagc acagtagata attatgttcg aacggcacat
     1981 tatctggatc aatcattgga acaatttttt acgtatttga aaaaatctgg tatttaccaa
     2041 aattcaatct ttgtgattta cggggatcat tttgggatct cgaatacaga taataaagat
     2101 ttagcaagcg ctcttggtaa agatccagat acatgggatg aatttgacaa cgcacagatg
     2161 caacgagtgc cattgatgat ccatatgcct ggttatacta aagggacagt caatcatgaa
     2221 tacggtggag aaatcgatgt tttgccgaca ttactacatc tattgggaat cgatgataaa
     2281 gattatcttc attttggtac agacttatta tcttctcagc atgatcaagt agttgctttt
     2341 cgaaatggga actttgtgac acccaaatac accgtcctag gaggaaaagc ttacaataat
     2401 cagacaggag aaatcatcga tacgaaagca gcaggaatcg atgaggaaat ctccaaagat
     2461 caggaaaaag tcaagactgc gttaagtctt tcagataagc tgaatcagga aaatctcttg
     2521 cggttttatg tgccaaacgg atttgagacg attgatccga aaaaatatga ctataaaaat
     2581 gaagcggaac gtaatgaagc aatcgaaaag caaaaaggcg aacattcgac aagtgtcttt
     2641 tcaaaaaatg gtaataaaac aacaaccaac ttatatccag taacaagaac agacgttgaa
     2701 cagcaaaaaa ataatcaata aacaatcaaa ccaaccttca gtagttagac aacattctaa
     2761 tgcttgaagg ttggtatttt ttatgaaaaa aacatttttt tatttttttc gtgtattttt
     2821 cctttttaac gtatctattt attgaatgtg gaaaaattta aattaaaaag gagaactatt
     2881 atgtatgatg gtgaattgat caagaaactt cgagtcaaca agaagttgac acagtcccaa
     2941 ttagcggaag gaatctgttt taagacttct ctagtcggga ttgagacaaa ttcagtgaaa
     3001 aaaatgtcat ttgtaacatt gagatcattt ttagagcgaa tgaacatgac acttgcagaa
     3061 tatgaatgga tgcgaaacca gctggatgaa ccaataaagt taaagaaaaa aagacgaatg
     3121 ctagatcggg tacaagaaga cagctttgat ccttataaag agatcagcaa taaccgtagg
     3181 cattttaaga aaacagaaga tctgtattat ttggttttga atcttcacat ggtcgaaaaa
     3241 acagaagtat ctgagacaga atttgaattt ttacgttatg aatgccgaat cattgaagaa
     3301 tacttgatgc agataccaga ctatggtcaa tttgaactat ctattctagc tgattttcct
     3361 tggatattca gtgatcggtt cattgagaac aattatttga aaatcaaaaa aagaatgcga
     3421 cgattagcac agttgaacaa tcatgagcag tatctcttta cttttttaat gaacctgact
     3481 tcgttttata tagaaaatgg tcggttgaaa aaggcacgta gtgtgaatga agatatgaaa
     3541 cggagcttag cacataaaga aagaagtaca gtgatctatg aaacattgat ggcagattat
     3601 tatcaaagat tgatttctgc tgctttaggg gaagaaaaga cgaaagacta ccaagaattc
     3661 ttcacagtgc tcgaatatat gttaggaaag aatcaacgga ggtccttaga gaaaaaactg
     3721 ctggccattg ctgaacccag caactagacc agcagtttag aatttgtatc acttcgccgc
     3781 tttttcagac ggtttgttta tatcgaatgg cgggaaaaag cgggcgatct cttttaccgc
     3841 agcatcacgg gaatcagaag cgtgaatgat gttttcagtc cccggcagcg cgtagtctcc
     3901 tcggatcgta cctggaacag catcggcagc ttttgttgct ccaaccatct tacggacgat
     3961 atcgatcact tcctcgccga ccagcacaag ataaacgaca ggaccggaag tcatgtaatc
     4021 gatcagttca tcaaaaaaac tgcgctcagt caagtgctga taatgttctt ttgctaattc
     4081 aggagtcata gtttcaaact tcatttcagc aatggccagt ccctttgctt caaaacgttg
     4141 gatgatgctt ccgactaaat ggcgtctcac gccgtctggc ttgataataa caagtgttct
     4201 ttccataaca gcacctcgtt tcctactata tttctattat agcgggaggt ggaaaatgag
     4261 acaaataaaa ccgcgtgaga caggaatata acgtgtgtat tttaaaaaaa gtatgaaaaa
     4321 catggaaaat tgatttgaca tcctcttttt tagttgttat attaacgaag gaaaaccgaa
     4381 taagatttgt taggtgaggc tcctatacaa acataggcta ctgcccaaaa acgtcgagag
     4441 acgccaatgg gtagaacagg aattgtcgag ataaggcttt tcataaggta gctaaaagca
     4501 ggtatctgct ttttacgttg tacagtgtca aaactcaacg atgagatctg agaagctttt
     4561 tttaatatgc tttttcaaac ctttcgctga gtatttggcg agaggttttt ttgttgccgc
     4621 aaatccccat cggtgaaaaa taaaaaggag gtggtctaat tggaaaaata actattcgaa
     4681 aaatgattgg aagtcatgaa ttaaaattac ttagtaaagg agatgagaac aaatggatga
     4741 cagtcattcg aaaaacaagg atctagagga aaggccaggg ctattatcta tctgttctca
     4801 gcccctttca gttttttctc tatcgatccg atgaatagct aaagaccaaa agcagcgaga
     4861 acagctgtct tttggacaca aaggagagat tgagatggaa aaaaagaaag atacacaaaa
     4921 aatctatgca gcttttgctg gaaaaacgaa acaagaatta tttgaactat tccatgcatc
     4981 agcaaatgga ttgacagaag aacaaatcga acgttcaaga gaaaattacg gtgaaaatgc
     5041 gatttcatat ggcaaaaaaa caccattcat tgtagaaata ttgaaagcat acatcacgcc
     5101 atttacatta gtgttgatcg cattaggaat tatttcattc atcacggaat atgtattagc
     5161 agctcctgga gataaagatc ttttcggtgt agccattatc ttcactatgg ttatcgtcag
     5221 cgggacaatg acgttgattc agtccgtacg ttccaatcaa gcggctgaaa aactgaaatc
     5281 acttgtgaaa gtaacggcag ctgtaaaacg taatggaaag tacacagaga ttcctatgga
     5341 agaaatcgtc tgtggagatc ttgttcggct ttcagcaggg gatatgatac cagcggatat
     5401 gcgtttattg tccgcaaagg atttattcat ttcccaagca gcattgacag gggaaagcta
     5461 tccagtagaa aagcatgcga aagtatgtga agacgatcag caaagcgaaa caagttacga
     5521 aaatcttgtc tttatgggaa gtaatgtcat cagtggaagt gcggaaggaa tcattgtttc
     5581 tgttggaaat gaaacactat ttggtcatgt agcagaaacg ctatctgaaa aaccaattca
     5641 atcaagcttt gaattaggca tccataaaac atccatgcta ctgatcaaat ttatgtcatt
     5701 gatggctcca gcagttatcg tcatcaatgg gcttacaaaa ggggattggc tagaagcttt
     5761 tctttttgga ctatctgtag cagttggtct aacacctgaa atgcttccaa tgatcgttac
     5821 gacgaatttg gtcaaaggag caagtgtgat ggccaaaaaa ggaacagtga tcaaaaattt
     5881 gaatgccatc cagaactttg gtgcaatcga tgtgttatgt acggataaaa cggggacatt
     5941 gacacaagat aaaatcattc ttgaatatca tatggattgt gaaggaaatg aagatgatcg
     6001 tgttttgcgc catgcgtatc taaatagcta ctatcaaaca gggctgaaaa atttgttaga
     6061 tgtggctatc attgatgaag caacagaaac tttagataca gataagatca attatcaaaa
     6121 agtggatgaa attccttttg attttgaacg tcgtcgtatg agtgttgtgg ttgctgatcc
     6181 agctggtaaa acacaaatga tcacaaaagg cgcgattgaa gaaatgctga acatttctaa
     6241 atttattgat cttggcggta ctgttacacc attaacaaaa gaacgaaaag aagccgtact
     6301 tgcaaaagta cagtcactta atgaagatgg cttacgagtg attggtgtcg cacaaaagac
     6361 caatccaagc gtcgttggag aattttcagt caaagatgag tcagacatgg ttttgattgg
     6421 ttatcttgct ttccttgatc caccaaaaga aacaacgaaa caagcgttga aagctctgaa
     6481 agaacatggt gtagctgtaa aagttctgac tggggacaac gaattagtta cacgttcagt
     6541 gtgccgtcaa gtaggtctag agatcaatga attggttacc ggtgagaaaa tctcggaaat
     6601 gagtgatcat gaattagcac aagtagctga agaccatgaa gtattcgtaa aattgaatcc
     6661 tcagcaaaaa gcacgtttga cgacagcttt acgtaaaaat ggtcatactg tcggattttt
     6721 aggagatggt atcaatgacg ctcctgcaat gaaagtggct gatatcggta tttctgtaga
     6781 tacagctgtg gatattgcga aaggatctgc agacgtgatc cttttagaaa aagatttgac
     6841 gattttggaa agaggccttc tttctggacg agaaactttt gggaatatca tgaaatatat
     6901 caaggctaca gcaagttcta attttggcaa tatgttttcc gtgctgattg caagtacgtt
     6961 cctgccattt ttaccaatgc tgcctttaca acttttgttt ttgaatctga tctatgatgt
     7021 ttcctgcatt tccttgccat gggatcggat ggataaagaa tatttggacg agccaaaaaa
     7081 atggaatgca tctagtattg gggaattcat ggtttacttt ggtccaacaa gttcgatatt
     7141 tgatattact acttacttgt tgatgtattt tgttatttgt cctgctgtag tgggcggaag
     7201 tttccataca ttggatagtt cacagcgagt gattttcatc gcattattcc atgcaggatg
     7261 gtttgtcgaa tcactatgga cacaaacctt agtgctccat gcactgcgta caccgaaagt
     7321 accatttatc caaagcaacg caacatttgc catgtttgtt atcactacat taggaattat
     7381 tgtcggttct gtccttcctt ttacaagttt tggtgctgaa ttaggcttga tgccacttcc
     7441 tggcaattac tggttatggt tatttgctac agtcatcgct tatctggcgc ttgtgacagc
     7501 tgtgaagaaa atctatatcc gtcgattcca agaactctta taaacgagaa ggctggttct
     7561 ttgcttctgt tctttttgga ccagtcgtgc taaactatgt gtattgtttt ggaaaggatt
     7621 aatgtataga tgaaaggtgc aatatttgat ttagatggtt tgttagttga cacagaaaaa
     7681 acgtatcgtg atggctggct ttggggattt cagcaatttg atatccatat cccaaaagaa
     7741 atcgttgacg cttggggcgg aaaaaactgg aaacaaagtt ttgatatttt agtgaaagca
     7801 gcaggtagcc ctgaaaaagt acaggaagtc cgagcgaagc gtgaagaata tttttatcaa
     7861 cagctgacaa gcggagccat ccacttaaaa ccatatgcaa aagaaatatt ggcagactta
     7921 aaagaacgtc atatgaaact aggcattgct acgaccactg ttacgaaaag agcaacagat
     7981 atcttggatc actttaaatt agctgattac ttggacacgt ttacattcgg agacgaagtg
     8041 tcggagaaca agccttcacc gattccttat ctgacagctt tggaacgaac aggattagaa
     8101 gctagttcag cctttgcagt ggaagattct ttagtaggag caacagctgc ttcacgagca
     8161 ggattaggcg ttgttttgat acctgattcc agttttgtcc gaaattatac cgtcgaagaa
     8221 aaggaaaaat tgaaacttct tgcggaagga aaagatcttc gaactgttat tgaaatgtta
     8281 aataaaaaaa cagaaaaata aatatagaaa aaataattt
//
LOCUS       DAFCLG010000111         8137 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFCLG010000111 DAFCLG010000000
VERSION     DAFCLG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.111"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            186..887
                     /locus_tag="LND56_002281"
     CDS             186..887
                     /locus_tag="LND56_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6744158.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1459..2475)
                     /gene="tsaD"
                     /locus_tag="LND56_002282"
     CDS             complement(1459..2475)
                     /gene="tsaD"
                     /locus_tag="LND56_002282"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK6744159.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2472..2936)
                     /gene="rimI"
                     /locus_tag="LND56_002283"
     CDS             complement(2472..2936)
                     /gene="rimI"
                     /locus_tag="LND56_002283"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6744160.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2943..3485)
                     /gene="rimI"
                     /locus_tag="LND56_002284"
     CDS             complement(2943..3485)
                     /gene="rimI"
                     /locus_tag="LND56_002284"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6744161.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3469..4191)
                     /gene="tsaB"
                     /locus_tag="LND56_002285"
     CDS             complement(3469..4191)
                     /gene="tsaB"
                     /locus_tag="LND56_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK6744162.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4382..5362)
                     /locus_tag="LND56_002286"
     CDS             complement(4382..5362)
                     /locus_tag="LND56_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6744163.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5386..6870)
                     /gene="galT"
                     /locus_tag="LND56_002287"
     CDS             complement(5386..6870)
                     /gene="galT"
                     /locus_tag="LND56_002287"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBK6744164.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6882..7871)
                     /gene="galE"
                     /locus_tag="LND56_002288"
     CDS             complement(6882..7871)
                     /gene="galE"
                     /locus_tag="LND56_002288"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK6744165.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2312 a         1726 c         1416 g         2683 t
ORIGIN      
        1 ttttttatac acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt
       61 acataataac aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa
      121 aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact
      181 ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt
      241 ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt
      301 ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga
      361 ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact
      421 atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt
      481 atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa
      541 aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag
      601 ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa
      661 aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca
      721 ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa
      781 ctgctggaca agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc
      841 gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat
      901 aagcagtgac cctgatattt atcagggtca ctgcttattt tcctccctaa aacttctgaa
      961 agatccggca gttttagatc actgggcaag actcaaagat tttctatgtg tgtttcagtt
     1021 atgtaatatt cactgtttgc aaaaaatact gatagattaa ctagaggaag cgtcttgtcc
     1081 tcatccgacc tagattattc gatattcctc gacaataaac gattaatcag tgagggcaat
     1141 cagaggaacg gataatgatg aataagctgt aaaaaatagg gctttgacaa aagcgtgtca
     1201 cagcccttat ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg
     1261 ttcataaaca tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca
     1321 ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag
     1381 ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc
     1441 agatttttca caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata
     1501 gctagcaaaa tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt
     1561 atctccacaa agtcgaagag ggggaatcac tagttctacc tcagggagtt tagttgccaa
     1621 agcatcctgc aatccttcac gcaacccttg attggccgct acacctccag ctacaatcag
     1681 ttgcttgact ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac
     1741 acttgcttga aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc
     1801 attatgcacc aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc
     1861 ttcatggatc atcgctcgtg gaaaatggta attatctttt ccctgatgag ctagctgatc
     1921 gatttctttt ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc
     1981 tcctgctgcg tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata
     2041 gactaattct gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt
     2101 aaccagacgg gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg
     2161 gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc
     2221 tggtccataa gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag
     2281 tgcatcttcc aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggaac
     2341 tacgccacca aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc
     2401 tgttccattt ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat
     2461 caattcttca ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct
     2521 tccgtcggat tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca
     2581 taaagttttc ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct
     2641 tttatttgtt ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct
     2701 gaaactgcaa cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata
     2761 aagccacaga tctggttatc ttcaactaaa agcagataat cagagtgagg ctgttggatg
     2821 tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc
     2881 atccatacgc attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa
     2941 tctcactcat tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa
     3001 tatctttttt tgatcgttgt tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct
     3061 tgattactca tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc
     3121 tcgatttcgt caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca
     3181 atattcgtga tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt
     3241 ccattatcaa agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag
     3301 gcactttttg tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct
     3361 ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgattgtt
     3421 ttttctgtat aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc
     3481 aacataactt tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg
     3541 ttttaaatag cgaggaagaa aagcttgttt ctctgcaact ggttcagcta aagcacctaa
     3601 ttgagccaca gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact
     3661 aggcaatatt tcctttattt catagaagaa tttgtgacaa tcactcccga cgaaataaat
     3721 agcttcattc agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc
     3781 aagtacaatt tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct
     3841 tcgagcatca aacatgggca caataatttt atccacaccg atgcaattag ccgcgatggt
     3901 tttcagacta gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt
     3961 cgttacacct atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc
     4021 aatgtctttt ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt
     4081 tacactgtgg ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac
     4141 cacaccgatc gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat
     4201 cctttctgct atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa
     4261 aataccaaag gcaaaactag tacaatgata catgattatc tgctggcggg caaattttgc
     4321 cccttcctga aacacaaaaa agctgcggta tcacttcatt tccccacagc ttttccatct
     4381 tttatttcgt tgattctcga ataatcaact ctgtttccac aataatcttt cttgaaaccg
     4441 gtgctttgga attacttaat tgaagaatcg tctccactgc tagctctccc atccattcgg
     4501 tataaacttt tacagtagaa agagagggcg ttacatattt cgctacactg acatcattga
     4561 aacccatcac agaaatatct tctggtacca ctaaaccttt ttcttgaatc gctttcagtg
     4621 cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt
     4681 ttaaatactg cttcactgct tgatatcctg actcgacaga aaaatctgct tcgataattg
     4741 gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa tgccataatt ctgggatctt
     4801 ttagccgctg atggtttttc ttcgtatatt ctgttccagc aagcatagcg atatgttgcg
     4861 ctttatggga gacaaaatgt tcaatgactt tttgaacact tgacttaaag tcgacaacta
     4921 gagagtcgat tccttgagaa gtgccgtcag aatctaccaa aagcaaggca ttttgggatc
     4981 ctttcatttc taataactga gcttcgtcga attttcctag tgcgatcgtt ccatctgtcg
     5041 gaagatcact aatggaatcc cacgtttctt tcaataaagt aacgtttgct tcttctgcct
     5101 ttttttcgat tcctagacga atagataaat aataaagatc ttcaagctct tccgtatcgt
     5161 cgtaccactg gatcaggcga agcgtttgct tagtatttgc tgcattttta cgatgtttcg
     5221 tataatttaa gcgttctgct gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa
     5281 gatcaggatc atagttgaga actcttgaaa cagtagcagg agaaactcct gccaactgcg
     5341 caatgtcttt aattgttgcc atttgtctct cctccttttt tacgtttata aaattgcagt
     5401 aaatcgttca aacgctgcct gtcctttttc atctcgcttg aatactccag cgtcttccag
     5461 tacacgtgca aaaacctgac ctacagattt ttgtacgaca tcggtgaccg tatctttcgt
     5521 caaatcagga tgttcttttt ttaattgttc tgcccacgga cgatgatatt cagccatctg
     5581 gttttcttct cctaataaga aatgctctac ttctactagt tctggtttca aacgaggtgg
     5641 taaaacagca agccccatta cttcgatcaa cccaatgttc tcctttttga tatgttggac
     5701 atctttatgt ggatgaaaaa tcccatcagg atgttcttct gaaacattgt tgtctcgaag
     5761 gacaaggtcc aactcaaagt aattgtccct tcttcgtgca attggcgtaa tcgtatggtg
     5821 tggtgtacca tctgctgaaa aagctctgat agatactgtt tcatccgaat atgctttcca
     5881 cttttccaat actgagttag ctgcttcaat caacaatttt ttctctgttg attgcaaacg
     5941 gatcacagac atcggccatt tcacgactcc tgcgatgact tgcggataat ctgctagttt
     6001 gataagtttt tctatcggtg cttttgccat aggaaattca tgacgtcctg cctgataatg
     6061 atcatgagaa agaatcgatc ctccaacaat cggcaagtca gcattcgaac caacaaagta
     6121 atgaggcaag acttcagtaa tccgaagcag tctagcaaat gtttcctttg tgattttcat
     6181 cgggcggtgt tgttctgata aaatgatcga atgttcattg taataagcat aaggagaata
     6241 ttggaatccc cagctttcac cgtctagatt cattcgaata atccggtgat tggtgcgggc
     6301 tggatagttc aagcgacctc tatatccttc gttttccatg caaagcatgc aggtaggata
     6361 gtctactttt accgcctcgc gttctgctgc gatttgtttt ggatcttttt ctggcttaga
     6421 taaattgatt gtgatttcca gttcaccata ctccgactct gccggaaatt tgatgttttt
     6481 tgcgatggct cttgttttga tgtagtcatt tttcttactt aaattaaaga aatactcagt
     6541 tgcttcctct gggcttttcg aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg
     6601 cggtgtgaga aaatccatta attgcgcttc aaaaatctct tgttctgctg gcaattctga
     6661 aagaacacca ttttctcttg ccttagcgac aagctgatcc aataagtcta cagattcttc
     6721 cttaactggt cttacttcaa tgctttctag agcatcttct cctaccatcc ctaaaagacg
     6781 attctgtaaa tataagcggt ccatttccat ccatccgcca gcttcgatcg caagtgtggc
     6841 aaaatcacaa atcgtttgac tgattgacat atctcattcc cctattcttc ataaccattt
     6901 ggatggctga catgccaatc ccatgctgtt ttgataattg ctttgatatc tgtgtattca
     6961 ggtttccaac ccagaacctc tctcgctttt tcacttgaag cgactaatcg gctaggatct
     7021 ccagctcttc ttggtgctat ttctgctgga atttcttttc ctgtcacttc tctagctgct
     7081 tctagcattt ccttgactga gtatcctttg ctgctgccta aattgaagaa gttgctttca
     7141 tttccttctt tcaagtattc caaagctaaa atatgtgcag caatcaaatc ttctacttga
     7201 acataatctc tgatacatgt tccatctggt gtatcataat cgtctccata aacagccaat
     7261 gcttttcttt gaccaagagc tacttgtaaa atgattggga caagatgcgt ttctggtgta
     7321 tgatcttctc cgatagatgc atctgctttt gctccggcta cattgaagta gcgcaaagcg
     7381 acatagcgca tgccatatgc ttggtcacac cacttcatca ttttttccat catcaattta
     7441 ctttcaccat acggattttt aggattagct ggtgtatctt ctgtgattgg agactctttt
     7501 ggttctccgt aagttgctgc agtagaagag aacacgattt tattcacatt gaactcgtgc
     7561 atgacttcaa gtaagatttg cataccgtat acgttgttgt tgaaatacat caatggtttt
     7621 tcgacagatt ctcctactaa agagcttgca gcaaaatgga ttactccttc aatcggttcc
     7681 ttttcaaata cggaacgtag aaattcttta tcccggatgt ctccttcata aaaatgagca
     7741 tctggatgta ctgcttgttt gtgacctgtg agcaaattat ctaccacagc tacttgatac
     7801 cccttttgaa ctagctggtc tacagcgtgc gaaccgatat aaccggctcc gcctagtact
     7861 aaaattgtca taaaaaacac tcctttgatt agtaaaattg tgtttgttga ttatatataa
     7921 tttaaaaaaa cgaagtttac ttttatggtg aaaacaaaca aagtaaaatt tcgtaaattc
     7981 atatggtaaa tttataataa aacatttagt aaacattttc aaggattttt tgcaaaggtt
     8041 tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt ccataataga tataacaaat
     8101 aataaatgag gtggattcaa tgaaaaaatt tgtatct
//
LOCUS       DAFCLG010000112         8067 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFCLG010000112 DAFCLG010000000
VERSION     DAFCLG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.112"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..884
                     /locus_tag="LND56_002289"
     CDS             <1..884
                     /locus_tag="LND56_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK6744166.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="LND56_002290"
     CDS             932..3061
                     /locus_tag="LND56_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBK6744167.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="LND56_002291"
     CDS             3036..3725
                     /locus_tag="LND56_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744168.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4818)
                     /locus_tag="LND56_002292"
                     /pseudo
     CDS             complement(4133..4818)
                     /locus_tag="LND56_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(4954..5148)
                     /locus_tag="LND56_002293"
     CDS             complement(4954..5148)
                     /locus_tag="LND56_002293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744169.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="LND56_002294"
     CDS             complement(5198..5638)
                     /locus_tag="LND56_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBK6744170.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="LND56_002295"
     CDS             complement(5642..6487)
                     /locus_tag="LND56_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBK6744171.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="LND56_002296"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="LND56_002296"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBK6744172.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaa
//
LOCUS       DAFCLG010000113         8036 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFCLG010000113 DAFCLG010000000
VERSION     DAFCLG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.113"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="LND56_002297"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="LND56_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6744173.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="LND56_002298"
     CDS             1228..2331
                     /locus_tag="LND56_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6744174.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIDSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="LND56_002299"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="LND56_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK6744175.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="LND56_002300"
     CDS             complement(3821..6268)
                     /locus_tag="LND56_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6744176.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="LND56_002301"
     CDS             complement(6457..7893)
                     /locus_tag="LND56_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6744177.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2236 a         1739 c         1374 g         2687 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattgattc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa atatgtaaag
     7981 gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaatgg
//
LOCUS       DAFCLG010000114         7765 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFCLG010000114 DAFCLG010000000
VERSION     DAFCLG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.115"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            363..731
                     /locus_tag="LND56_002302"
     CDS             363..731
                     /locus_tag="LND56_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK6744178.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="LND56_002303"
     CDS             911..1852
                     /locus_tag="LND56_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK6744179.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="LND56_002304"
     CDS             1921..2652
                     /locus_tag="LND56_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBK6744180.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="LND56_002305"
     CDS             2796..4976
                     /locus_tag="LND56_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK6744181.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="LND56_002306"
     CDS             4996..5478
                     /locus_tag="LND56_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBK6744182.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="LND56_002307"
     CDS             5494..6255
                     /locus_tag="LND56_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6744183.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="LND56_002308"
     CDS             6386..7720
                     /locus_tag="LND56_002308"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK6744184.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2604 a         1344 c         1706 g         2111 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtg
//
LOCUS       DAFCLG010000115         7700 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFCLG010000115 DAFCLG010000000
VERSION     DAFCLG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.116"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            544..1107
                     /locus_tag="LND56_002309"
     CDS             544..1107
                     /locus_tag="LND56_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744185.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="LND56_002310"
     CDS             complement(1386..1673)
                     /locus_tag="LND56_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6744186.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="LND56_002311"
     CDS             complement(1663..1992)
                     /locus_tag="LND56_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6744187.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="LND56_002312"
     CDS             2297..2626
                     /locus_tag="LND56_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744188.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="LND56_002313"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="LND56_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK6744189.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="LND56_002314"
     CDS             complement(3551..4462)
                     /locus_tag="LND56_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK6744190.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="LND56_002315"
     CDS             complement(4890..5498)
                     /locus_tag="LND56_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK6744191.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="LND56_002316"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="LND56_002316"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6744192.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="LND56_002317"
     CDS             complement(6528..7496)
                     /locus_tag="LND56_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6744193.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2351 a         1532 c         1281 g         2536 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatta
//
LOCUS       DAFCLG010000116         7688 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFCLG010000116 DAFCLG010000000
VERSION     DAFCLG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.117"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="LND56_002318"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="LND56_002318"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK6744194.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="LND56_002319"
     CDS             complement(2032..3285)
                     /locus_tag="LND56_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6744195.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="LND56_002320"
     CDS             complement(3291..4214)
                     /locus_tag="LND56_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6744196.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="LND56_002321"
     CDS             complement(4435..6927)
                     /locus_tag="LND56_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK6744197.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="LND56_002322"
     CDS             complement(6934..7401)
                     /locus_tag="LND56_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBK6744198.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="LND56_002323"
     tRNA            complement(7537..7610)
                     /locus_tag="LND56_002323"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
BASE COUNT         2137 a         1669 c         1236 g         2646 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaac
//
LOCUS       DAFCLG010000117         7626 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFCLG010000117 DAFCLG010000000
VERSION     DAFCLG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.118"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..706)
                     /locus_tag="LND56_002324"
     CDS             complement(14..706)
                     /locus_tag="LND56_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6744199.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LND56_002325"
     CDS             complement(1143..1712)
                     /locus_tag="LND56_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6744200.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LND56_002326"
     CDS             complement(1786..2109)
                     /locus_tag="LND56_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744201.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LND56_002327"
     CDS             complement(2263..3075)
                     /locus_tag="LND56_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744202.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LND56_002328"
     CDS             3429..4277
                     /locus_tag="LND56_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK6744203.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPLR"
     gene            4422..5363
                     /locus_tag="LND56_002329"
     CDS             4422..5363
                     /locus_tag="LND56_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBK6744204.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LND56_002330"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LND56_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1370 g         2472 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattccttt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctt
//
LOCUS       DAFCLG010000118         7597 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFCLG010000118 DAFCLG010000000
VERSION     DAFCLG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.119"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(940..1353)
                     /locus_tag="LND56_002331"
     CDS             complement(940..1353)
                     /locus_tag="LND56_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBK6744205.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="LND56_002332"
     CDS             complement(1809..2294)
                     /locus_tag="LND56_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744206.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="LND56_002333"
     CDS             complement(2433..2648)
                     /locus_tag="LND56_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBK6744207.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="LND56_002334"
     CDS             2872..3672
                     /locus_tag="LND56_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6744208.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="LND56_002335"
     CDS             3691..5559
                     /locus_tag="LND56_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6744209.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="LND56_002336"
     CDS             5552..6043
                     /locus_tag="LND56_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBK6744210.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="LND56_002337"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="LND56_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK6744211.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="LND56_002338"
     CDS             6603..>7597
                     /locus_tag="LND56_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK6744212.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAFCLG010000119         7473 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFCLG010000119 DAFCLG010000000
VERSION     DAFCLG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.120"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..985
                     /locus_tag="LND56_002339"
     CDS             <1..985
                     /locus_tag="LND56_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK6744213.1"
                     /translation="TYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDM
                     IGGVFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKV
                     FIPELFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNF
                     TIQGLLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFV
                     SEFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKV
                     IAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            982..1701
                     /locus_tag="LND56_002340"
     CDS             982..1701
                     /locus_tag="LND56_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6744214.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1716..2801
                     /locus_tag="LND56_002341"
     CDS             1716..2801
                     /locus_tag="LND56_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6744215.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2937..4469
                     /locus_tag="LND56_002342"
     CDS             2937..4469
                     /locus_tag="LND56_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6744216.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4647..4868
                     /locus_tag="LND56_002343"
     CDS             4647..4868
                     /locus_tag="LND56_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK6744217.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            5450..6067
                     /locus_tag="LND56_002344"
     CDS             5450..6067
                     /locus_tag="LND56_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6744218.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            6064..6594
                     /locus_tag="LND56_002345"
     CDS             6064..6594
                     /locus_tag="LND56_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK6744219.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
BASE COUNT         2657 a          799 c         1434 g         2583 t
ORIGIN      
        1 aacatattta acaattaaag attactataa agtattcgct ataattgaaa aattattctg
       61 gatttcactg ccactttgtt taattgaaag attcatggtc acctatccaa ctggaacaat
      121 tgtaggagat atgataggtg gagttttttg gaattattca ggttgtaata tgccattgaa
      181 tttaattttg tgtgtgacag ttactgttgt tacaatcgga tatttcaagg gagaaaaaaa
      241 tttcatccat tttattttat gtgtttgtgc agcacttttt atgtctgcat tagctgaatt
      301 gaaagttttt attcctgaat tatttataat atttgttgca atagtattca ctttaaaagt
      361 tgattttaaa gtagtgatca agatattatc atgcgtaata cttgtgtcga tagtattatc
      421 cttttttata acaatattta ttacattgaa cgataatgga caaagctatg ctgataattt
      481 cacaattcaa ggtcttttag aatatgcgtc aagagattct ggatatgatg gtgttggtga
      541 tttaaatcga ataactggta tctctagagt agtagatgaa atatttaata ataattttag
      601 attagtccta atgggagtag gcattggcaa tgcagagttt accaattttt ttgtcagtga
      661 gttttataaa tcgtttggta atcttcatta tcagtatttt catcatattt ggttatttat
      721 agaagttgga atattaggag tttcgatatt cctatttttt ataataaaga catttttcaa
      781 tgcaaaaaga tcttttatga aggataaaga tgcaagtctt gttaaagtta ttgcaattat
      841 gtcggtattc ttttttgtgt ataacacaac acttaggggt gaaacaacgg ggccactgat
      901 ttatatgatt ttagccatgc ctgaagttgt gaaaaggaaa gagcattatt atagtgaaaa
      961 aaatacaaaa ggagagatat tatgattcct aagaagatac attactgttg gttcggggga
     1021 aatccacttc cgaaaaaaga tagaaagtgc atagaaagct ggaaaaagta ttgccctgat
     1081 tatgagatta ttgaatggaa tgaatcaaat tatgatttaa ataagaaccg ttatatggcc
     1141 gatgcatata aagaacgtaa atggggtttt gttccagatt atgcaagatt tgatattatt
     1201 tataatcatg gtggatttta tttagataca gatgttgagc taattaagtc gttagatata
     1261 cttcgtgaaa atcatgccta tatgggattt gaaggggaaa tctgggttaa tggcggtatt
     1321 ggttttggtg cagaagaagg acattctgtt attaaaggtt taagggatat ttacgattca
     1381 ttaagttttt atcaaaatga tggtacattg aacttgaaac cgagtccgta ttatattaca
     1441 gattatcttg aatctattgg attggttcgc gataacagcg aacaaataat aaatggcctg
     1501 catatctatc ctacggatta ttttgctgcc aaggattatg atactggaaa aatttataaa
     1561 actagtaata caataagcat tcatcaatat aatgcatctt ggaaaacacc taaacaaaaa
     1621 tttgtttata gattaaaatc aacaattgga caaaaaaaat ttaacaggtt acttgcactg
     1681 aaaaaatttt tacacttata aaggagaaaa gtactttgaa gaaaatttgt ttcattagtg
     1741 gtgtaatttc tcgttctggg ggaactgaaa gagtagggag tattattgcc aatagcttgt
     1801 ctgatttagg ttatgatgtc actattttaa gcttttggaa tagtggagag ccttattttg
     1861 ggttagataa gacgattaag gttgattata tgttaaatcc taaaacggag ggaaaattat
     1921 atagaactta tttatatccg gtttggaaat tgcacaagta tatatgcaaa aatgaatttg
     1981 atatagtaat tgatattgat actttgttgt ctttgtatac tacaagggca attaaaggaa
     2041 caaagtgcaa attgatttct tgggaacatt ttaattactg gacaatggaa atgctgaaag
     2101 aaaagaagag attcaaggct aaaaaattga taaaaaaata tgcaaagagg cttgtggttc
     2161 tcacagaaga agatcgtcaa aaacatattg atgaatatag tttatcagaa gattttgtta
     2221 taactatgcc aaatccgtgt atatctaata tcgagtacga atatcattat gataaaaaga
     2281 gatttcttgc agtagggcga ttagtggaag agaaaaattt tgctgacatg ataagtgctt
     2341 ggaaaattgt agaaaaaaaa gtagatgatt gggatcttat cattgtagga aaaggtgaat
     2401 tagaaaaaga tcttcgccag cagataaaag atttaaatct gcaaagagta actatagcag
     2461 ggcattcaga ccatgtggag gaatattata aatctgcatc ttgttatgtg cttagttctg
     2521 tttatgaagg atttcctatg gttatattag aagcacagtc ttatggatta ccagttattt
     2581 cttatgattg taaaactggt ccgagagatt tggtacatca taattttaat gggatgttgg
     2641 ttgaagataa gaatatagac caattagcta agagcatgat aatgtttact aagaatactg
     2701 atttagcaat gagaatgtca ctgaacgcat ataataatgt acaaaaattt aatttgaaag
     2761 aaattacaaa acaatgggtt gctttgattg aaaatgtata gttttttgtg gttgtccaaa
     2821 catgaaaatt agcgtaatta atcaaagagc tagaagaaac ctattacatt tcaagatttt
     2881 agaaattata tagtattagg taacaataaa aaatagaaag tttggagcca tatgatatga
     2941 taattaatcg taaacaaaat gctaaaagga atataattac tggaacaatt tttaagttat
     3001 atcagattat cattcccttt tttatgcgta cacttatgat ttatcagatg ggaatggagt
     3061 atgctggatt gaacaacctg ttcacatcta tatttcaaat gttaaattta gcggaattgg
     3121 gaataggtgc tgcactaact gtaagtatgt ataaacctat agcagaagac aatagaaaag
     3181 agatttgtgc tttactgaac gcatataaaa agtacttttc aattattgga tggatagtgt
     3241 ttacattagg attgttatgc attccttttt tgagattttt agttaaaggt tcgattcctg
     3301 gaaatttaaa tatgacagtt ttgtatttaa tgtatttgat aaatactgtt ttgtcgtatt
     3361 ggttattttc atatagaaag agtctaatat atgcacatca aagaaatgat gtaattaacg
     3421 tgtctatgct aattactttt acattccaat ttgtaatgca ggcatatgtt ctgattttct
     3481 taaaaaatta ctatttgtat ttattggctt ctattttggg acaaatagtt cttaacttaa
     3541 tgtgtgctgt tgtttctcaa aagatgtatc ctgattatac tcctgttggt gagttagaga
     3601 aagaaactat tgacgatatt ataaaaaaag taaaaggatt agttacaaac aaggtaggcg
     3661 gtacaatatt acgatcttca gattcaatta ttatttcatc tttcttaggt ctttcagtac
     3721 ttgcgattta tcagaattat tactttatta tttctgccat aatgagtata tttgcaataa
     3781 tttatgaatc atgtatagca ggtataggta acagtcttat tgtagaaagt caagaaaaaa
     3841 attatataga tttttgtgat attacaacaa ttataggttg gtttctggca attagttgtg
     3901 catgctttat tacattattt cagccgttca tgattatctg ggtgggaagt gataatctta
     3961 tgagtatttc atttgttata tttatgatag ttcatttttt cttatatgaa attgatcagt
     4021 taattgggtt atacaaagat gctgctggaa tatggtacgc agacaggttt agaccattaa
     4081 ttacagcaat gataaacatt gtattaaata tcatcctcgt taattggttc ggattatatg
     4141 gagttttggc ggcaaccatt gtttcagtat tgttggttga tttaccctgg ttattatata
     4201 atgttttcca aaatatattt aaatttgaat cggcaggtaa atatgtttgg aagctcataa
     4261 gtttgtgtgt aatagctttt gttgcttctt atttaagtta ttttgcttgt tcgtatgtta
     4321 atatcggaaa tttcaaagtg ataagtttag tgataaattt agttatatcg gttgtcatat
     4381 cgattttagt tattcttctg tttaatatcc gtaataagaa gtttaagtat attttgaaag
     4441 ttcttacgtc ggtattggta aaaagatgag aagagacatc agaatgatat ataaagtaac
     4501 tacaattttt aggctgccga ataggcgaac gatgtatagt ttttgttcca acgaagactc
     4561 ttgttgaaat tggagatgat atgaaggagt tacaattttg actcatgttt atgattgatc
     4621 ggttttaaaa gtagtatgga gaagctttgg gatcatcgga gaggatcaaa ataggtaata
     4681 atgtttttat aggaatgaag tctagaatac taaagggtgt acatatagga aataatgtag
     4741 ttataggtgc aaatagttta gtaaacaagg acgtatcaga cgattgtgtt gttgcaggaa
     4801 atctagcaaa agtaattatg tccctagatg aatattataa aaagataaac aatatgaaga
     4861 atccgtaaaa cttgtgaaac tataccgaga ataatatggg aaaaatattg atgaaaaagc
     4921 attatatgag tttttagggc tgtttacaga tggagatgac aatttgtcga agagttggga
     4981 gcaatgcaaa atttagtagg ggattataaa cagactgaca aggtatttaa agaacatgca
     5041 caagtatttc ataatattga agaatttttg aatactgtga gataggagtt gctacattga
     5101 gaaaagtatg tatttttcca atgtaaaaga ttacgagttt gaaaacgaca ccataatatt
     5161 attttttaag aagatattat agagcttagg aacggaagtt tatttaagga tgttgaataa
     5221 aatgaaaaat aagattaggt ggatgaaaaa ggcagatgat tttttactat aattaaaaat
     5281 attgttacta tgttatcaat cgtataaaaa tacttactcg ttatcaagac gattatttcg
     5341 aatattttag gaaaaataaa ttgaatatta gagaatgtga taatacactc taatatgatc
     5401 atgtgtaatt ggaagcgata agtgtatact tgatattgga gaaataatta tgataatagt
     5461 gattgcagaa attattacag atatattata taaattatat gaaccctttg ggtttgctgt
     5521 tttattgaca attttaggac tggtctattt tatgtatgca acagatggca ttgctgcagg
     5581 tgagggctat aaacgtatac taatggaact ttatcttcat ttaaagaagg atagaaaata
     5641 tagaaaagtt tgtgctttga tattctatac tgcgatgatt ttatttacaa cattaatcaa
     5701 tcgaacaatt tggttaaatc ctttatctga tgttatggga gactggtgga tttataagta
     5761 taatacaaca actggaacaa aagagttatc tacagcgtgt ttagaaaata ttattctttt
     5821 tattccatat gtttatatgg taatgtctat taaatatgaa aatataaaga aacccaaaat
     5881 atggtcctca atagtatatg gaggaaaaat tgcattttat ttttcagcaa taattgaatt
     5941 tttacaactt tttttgaggc taggaacgtt tcaaatttcg gattttgtat ataatacgtt
     6001 gggcggtata ttgggtgtaa tcctttataa actaattttg attacaaaaa gagatggtga
     6061 aagatgagac tcggattgat aataaagaat atattaaatt tacctaaaac gatatacttc
     6121 aattataaat ttcttcatat ttcagatgcg tggagattac ctattttggt gcatcgtaaa
     6181 gtacggatta cacgcaaatc gagagggaaa attgagatag tattacaatc tggaaaaata
     6241 gtttatttgg gctttccagt attaaatact gcgggaaatg aagaaacgat tataagtatt
     6301 gaaggaatac taagaataca agggccagcg tttatcgcac aaggggctca tattgacata
     6361 aggaaaaatg cagttttaga tattgggtca aagtttaatt cgacaggaaa aatgagtata
     6421 atttgcttta aggaaattca attcaaagat ggctgtacag tatcgtggga tacattattt
     6481 atggataccg attcacatac gatttttgaa ttgaatactc ataggatttt gaataaaaat
     6541 aataaaatag ttataggtaa acattgttgg attggagcaa aaagcgtgat atagaaaggt
     6601 gttcatttag cagataatat tgttgttgct tgtaattcca cggtagtaaa aagtcatgaa
     6661 aataataatg agattatagg gggggctcca gttcgaacgt tgaaaagaaa tacaagttgg
     6721 tgtttgaacg agccaaagtg aaaccaaata attataaaac tatggaagta taataaagtt
     6781 tttgagaaat ttgaacgaca agaccctaaa aaaatccatg gaccattatt atggatggac
     6841 ttcccaaaag gaatagccac ttttgagcac gtaaatcccc taataagtgg atcgacatcg
     6901 cttgtgcagc agatgatact atcaagaaag gtgtttataa tgtagaactt gaactaaata
     6961 gatttgtaat cacccattag gattgtaaag aatatgaata ggatatatct taagatgaaa
     7021 aaatttgcaa attctaatgt atatttatta agggcacttt gctgtgtttt tttcagagat
     7081 aagatgaaag aaagatggaa gtcttggagt atacgaaata aagaaaaata ttatatctgt
     7141 ttaataagtt gatattgaac aaaggactag tcggagtcag caacacataa gttgtgagtg
     7201 atgctgactc gtttttggca gaaaagaatc gaatagtaag taggtgtttt gatgttgctt
     7261 gacaatggag agagaaccca agaagattac tagcaaagct tatagatgtt actgtagatt
     7321 tttaactcca gatacagagt ttgatgaata ttctgattat atcaataaca ctataaaaaa
     7381 ggaaatttcc ttttcacagg agaaaggtaa aagatatttt cataggagaa tcaatgttaa
     7441 ttttaaatta tatgatttgt caaattaagc tag
//
LOCUS       DAFCLG010000120         7308 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFCLG010000120 DAFCLG010000000
VERSION     DAFCLG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.121"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..2138)
                     /locus_tag="LND56_002346"
     CDS             complement(39..2138)
                     /locus_tag="LND56_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6744220.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(2439..3263)
                     /locus_tag="LND56_002347"
     CDS             complement(2439..3263)
                     /locus_tag="LND56_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744221.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(3260..4015)
                     /locus_tag="LND56_002348"
     CDS             complement(3260..4015)
                     /locus_tag="LND56_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6744222.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(4028..6004)
                     /locus_tag="LND56_002349"
     CDS             complement(4028..6004)
                     /locus_tag="LND56_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6744223.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(6057..6728)
                     /locus_tag="LND56_002350"
     CDS             complement(6057..6728)
                     /locus_tag="LND56_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK6744224.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(6788..6997)
                     /locus_tag="LND56_002351"
     CDS             complement(6788..6997)
                     /locus_tag="LND56_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744225.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(7064..7303)
                     /locus_tag="LND56_002352"
     CDS             complement(7064..7303)
                     /locus_tag="LND56_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744226.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         2014 a         1497 c         1102 g         2695 t
ORIGIN      
        1 aaagaaaaca aggctgattc tcagccttgt ttttatattt atttgagttt taaaactaca
       61 tagcccaatg atgctaaagt aagaaataat atacttccaa taacagttag cactaaatga
      121 ttcgatccag tttttggcac aatgatctga ggtaagttag gttttacccc aatcgttact
      181 acattagttg ctttctcaat actttctact gaaacttttg cttggttgtc gatatccaat
      241 tcttcattca aaggagtttt agctgtaaat atcagctgaa tttctcgtcc atctaaaaga
      301 tttttactta agaaatcagc ggaagcttcc cacttcagcg ttcttgaatt gctatcatag
      361 gttagtgtac cttctgaagt aatatctttt cctgtactca tatctatcac tcgcgcagaa
      421 ccttctttat acgtcaaacg atcttccact ggatcttgaa tactaaattg cttgtaatga
      481 tctgaggcaa tgtctacacc ttttgtatgc catttttgtg tgactagata ttgtacttca
      541 gatcctgcat ttaaaggagc tgtaggcgtt ccttttttac catcatttcg accatcaaca
      601 tctttcccat cttgttccac ttttttattt ggtttaagag agaaatagtt ttctactgta
      661 tttgtttttt gtgttccttt gttcggatca ttgttaaacg ttacagtagc cacatttggt
      721 gcaattcctt ttgcatcatt ttttgctctc atatggagct gcaaaatata atccctaccg
      781 taatttgcag cgttagctaa aaagctgtca gaagcagtat aagtgactgt attacctgat
      841 acagttaact ttcctcctgt atcagctttt ttccaagtac cattcgcttc aacataccac
      901 aatgcgccat catagttttt cccatccgat accatatcaa aatttttgct gtcaaaggta
      961 tcagtgattt taaatgattt gtatttgttc attgtgttga ctgttaatgc atcaaacttt
     1021 tggtgaatat gatagtaaac atctgatcct tgtggaatca cttttttgtc aatattgtgt
     1081 gctgcctctt cagtattagc tttgttaaac acttcttttg taggcttcga tggttgtggt
     1141 gtccatagca atgcagtatc aaagctaaac atcatactat tcgtatttgc gccaatcatg
     1201 aatgttgggg aagcgccttg aagcgtatac gttacaccac ttctgtgata agtatcagct
     1261 cctacaacat cggtgaaatg atcattcgag ctataagcct tttctgcacg tcctcccact
     1321 acttttgaag ttaaattgtt tgttgataac gcaggagctt tgtattctgc acatgctgta
     1381 tctgttgtga caaaataagg taagctaccc attttttcat acccaacaaa ttctctcata
     1441 tcaatattac gttcaccgac agattcatta ttgtcattat accaagacac acgttgctga
     1501 tactgtccat ttaaactgta aatggataga aatgtatcgt ttaaatcaat accagctgct
     1561 ctcatcgatc tcatttttga ttgaatttct ggctgtggtt cactcaaagc tacttttgtt
     1621 tttgttcctg catacttaaa ttctagtttt gcattcatga cattgattga ctgcatgaaa
     1681 aatcctcgat aaatattttt atcaaagagc aaataattat aatacttttt gccattaatt
     1741 gttttagcta atccgtcttg gttaacccca gcgtgcgcta caaaattaga aaaagtcacg
     1801 tcacaatcaa tttcttttcc tagataagta cctacttttt gataatgggc aacgattttt
     1861 gacgtacttt tcaaatcttt gacttttggc caagcaagta ctttttgttt tttagttgaa
     1921 tactcatcta gtatcttaaa tccattgatc ccagttactt cgatatttct caatgaatca
     1981 ctatcatgat aaagcaccag attattattt tctttgattt gactcggatc aactttttct
     2041 aaggcctgga ctgcagttcc cctcgcaaaa agaagaagga acaaaaacaa cactaaagta
     2101 attactcttc ccgtagtgca atgaatttta ttttccataa tgccctccat ctaatatttt
     2161 tttaaacgat cgtaagcttt tcattctttt tttcctaata cccacgttac caatcctgta
     2221 agaagaacca aactaaccat aaaaattgga ttgatcaaga gctttttaat cacctctaat
     2281 ggcatggaag cacattgaat gtttagaaaa tgaatgaaca atcgttgata tcttggataa
     2341 attgataatt gagaaaaaat taaaccgcaa cacaatattg ttgcggctaa aaaggataga
     2401 ttaaaaacta atattcttat tattttcatc atttcatcct aattatcaaa aaataattga
     2461 gcaaaaaatt cccaacaatt ttcaaaaaag ttcttcttta caccagttga cacaactttt
     2521 ttaatttctt ctttcggctc tgatttctct gatttagggt ataaccaaac atccgtcaaa
     2581 aattcacctt tttcatttga aacaggtgtc aataaaacaa tatcttcact gtttgaaatg
     2641 ttttcagggc ttgattcatt aacgatatag tatgcttgtt ttttgtttgc aggtacttca
     2701 atggaaaatt caatcacgcc ttcctgatca tcaatcgttt tggtttctcc ttcagccact
     2761 tttttcaact ggtcgtatga aagttttttt gctcgatctt tcaattttga ctcaattatt
     2821 tctacagatt gatttttctc attatcatct tgtgacattt cttctaagat attcgtgata
     2881 tcgtagacaa taaacttcgc gccattgata ccttcttgct gggctacttg actgccttga
     2941 ttgttgattt gattcgaaga aataattttt tgaatatgaa tggcttgctt gatcacttgc
     3001 gtatcttcta cgtcttgcgg cttttctgaa gtctctgaag aactttctga cgttggattc
     3061 gttgattcag taaagcttat atttgaatct gccgtttctt ctgtagtatt ctccgaactg
     3121 tccgtttcgc ttggtgcagt aattgaatta tctattgttt gagtggtact actatcactg
     3181 ctgtcagcga aagacagttg cgaataagct acagtagaaa agagagctgc tacacaaaca
     3241 aacagccatt ttctcttatt cattccgatc tcctccttat ttttttataa ataaaaataa
     3301 taggaacgca aaaaaaagcg aatagcagtg ctaatcgcca atccaatgaa attgatttcc
     3361 tattttttgc ttttttctgt agttttaaat cttctttact gaaggaaact cgttcaccat
     3421 acactaaaaa gcggtgtgag ttgacacctt ttggtgtgca tgtcattaac gtgatcctat
     3481 cttggtcttt ttcaggcata agataatcta aataattttt ttgtaattct tctgctttaa
     3541 cttcttcaat tcgataaatt tgataggcat gttgttcttc atttacctta ataaagaatt
     3601 tttgcccttt ttttactgaa ggcaaatcat cgaaaagatt atctttataa agtccactgt
     3661 gccccgcgat taaaggattt ttaccgtttc ctcctgtaat atcgccagtt ccttccgtaa
     3721 tccctacacc attctctaaa attttgtcgg aagttccgtc ataaattggt agttttaaag
     3781 aaatacttgg aattgtaaga acaccaacag gatcgcctaa ctctattttc gcaacatcca
     3841 aagaagaatc tgttttttca agattttcaa tggctaaaat tctttgctgt tgttgaaatt
     3901 tcgctacatt tgtttccaca actggaatta aatagactgc tactgccgac agcaaccaaa
     3961 aaaatatcat cagtttgtgc atgactcttc tttttggggg cttacgaata atcattagct
     4021 gatccttcta ggctttcttc ttaaagtaaa caactgcaga aagtccccct gctcctagca
     4081 acaagcaaga gatcaatacg taagattgga aaccgttgct accagtcatt ggtacggaag
     4141 ctttttgatt atttttgatc gtgattcggt tttcttcttt gtatgaatct ttgtctactt
     4201 tgaaagagat ctcttttgtt aatttttgat agttttcagg agcttttgtt tcttccagtg
     4261 agtaatctcc ttcagttaaa ccagtgattt caaaatgacc ctctttatct gattttagga
     4321 ttgctgcatt tgctacatcg tctacccaaa ctgcttcttt gacaccatct ttatctgtat
     4381 cagtaagtcc accgtagtac ttaccttctg cattcttagc tgcaaattct gcgccttcaa
     4441 gcggtgttgc tttgatacct gttccaaata ggccgctatc gacttttaag aattttgcgc
     4501 cataagtcac aacgtcagct ttcgtttttt cgtggtgttc ttcctgatta aatggattat
     4561 tgttataaac gaaatccacg ctatttgtta atggatttgc gatttcagca ctatcattca
     4621 caaacatgtt ataagtcatt tttagatctt gaccatgtaa attcgcaact gtatcactta
     4681 cttgtccatt atttacgata aaatcgatca caaaaccact atcagaattt tcgattttat
     4741 agtcagtatt ttcttttaat acgactgaac catcattatt tgttacttta atatctgctt
     4801 tttcagcaac ataagaaagt cctttacctg ctgtatctcc ccatttaaat acactgtaag
     4861 gagaagtttc gcttttcaca ccgtttactg tgataccgtc tgcgccacct gcaatgtctg
     4921 ttggaatatg aatcgttcct tgataattta ctgcgtcacc aatttggaaa gaatgttctt
     4981 gtcctgttcc atttgttact gttttatcga tcactggtgc tttatagttt acgtatgaag
     5041 cttttaagtc acttgtcacg ccatcattcg taatttcaaa ggttaattta ttgtgcgcat
     5101 cgttgcgagc gtctgcgcct aataaatatt gatctgctgt tggttcttga tctgatccca
     5161 ctacaacttc cgaaggaact tccacaaaat agtattttcc taacgtcaaa tcagtcgcag
     5221 tcagctttcc ttgatcatcc gatacaagat cacccaattt tgttcctttt ccaggttcgc
     5281 ccttgtataa ctcaaatttt gcccctttta aaagggtttg tttgctttgg tctgcgccat
     5341 cgtcaccaaa tttcgttaat tcaaaactca acttagaaac aatgttctta ggatacaaat
     5401 gaatattttt taaaaatcct gatcctgttt tatctgtcat tggcgcaatc acaaccattg
     5461 gttttgcttt ttgtgtgact aatccagcag cggatttact ttcataaacc acatatacat
     5521 tccctttgga attgacatag gctggttgat cagtaaaagt gatctcacca ttttcatcta
     5581 cagctgaagt aattttgttg gttgcattct tgacgtactc actgttagat cccttgtctt
     5641 ctacatcttg tacaatctcg tccactttcg cgttggttag atcagaatct tctctcggta
     5701 atacttgatc cgtaatattt cctaacgtaa actcaacatc accataatct gccttgttgt
     5761 atttcgttac acctgtagga tattgatcgt gcgtataacc atcgtttttg atcccgtcaa
     5821 tatcaacatt caattgcgtt ccttgatcgt acattaattt atgaaccgtg atattgacct
     5881 tctctggtgg tgttttttgc gtgacatctt ctgcatgtac ttgatacccc tgaaagcagc
     5941 ctgcaatgat tcctaacatt ccaacaagca ttccagcttt ttttacatac cgatttattg
     6001 ccatttttta ctctcctcat ttcatatttc aacgactcgc gttgattaaa accttgttat
     6061 tgtagctctc cctgtaccac gtatctttct tttccttgtg acgtataaac acaagtaaac
     6121 aacgttaata agggtttctc tcctttcttt ggcaaagaca agaaagagtt ctcttcagga
     6181 acgccatcga ttaatttaaa gtacccaggt acgataaatt tacgcgttac cttgtaacga
     6241 tagacgccat cgttactaac taagtccatc acctccccta atgacaactt ttgaatatca
     6301 gaaaataaaa cacctggcat ttccatgtta tgacctgcta ggacatagtt tcctttgccc
     6361 atttcggcat cttcataaaa ataagtagcc gctccaagac tcaaagtgta ttgatttgcc
     6421 cctttaaaaa ttggcaatgc taaaccaagt gcaggaatct gaatttgtgc aaatacttgt
     6481 ttattgacgc ccatttcgat tgcttctttt tgtatggaca acatttgccc caaagacata
     6541 tcattattga cgttcgttgc atcctcctta taactctttg catgtgcatt gatttgatct
     6601 acgggcatct ttttttctat attttgctta aacacctctc cttcatgttt ttggtagcta
     6661 acatagccaa acacactgca aaagacgatt aaagtgactc ctaaaaaacc aaataaccat
     6721 tttttcatcc atctgcctcc tttcagacaa aacaaaaaaa ctttttctca tttccataga
     6781 aaaagtttta aaaatgaatt ctataagttc gtctcaactg ttcaaagttt ttcattgaac
     6841 ctaacaaaca agctttctct tttcctgtta actgcatatc attgatatat tgcgcgacag
     6901 ataatctttt aaggtgtttt tgcttcatcg tttttagtaa cttttcttcc atttctttcg
     6961 acactttttt tctgttttcg atttcttcat aattcatgag ttttcctacc ttccttgttt
     7021 tttgcaaaaa taaaaaagcc tatgatcaca tacgctctac acttcagtct tgggttatct
     7081 ttttctttcg tttaattact ggccgtgctg gtttcaaatc cttgattcca ttatgggttc
     7141 tccaataatc gaattctttt cgcaatcttt ctttttccat atctgttcct ttagataagg
     7201 ctttgaatac ttctttaggt gaataaccac gagctaacag ctgtacatac cttccttttg
     7261 tcaaagtatc catatccatc gcctctaaaa ctggtcgcgc catctact
//
LOCUS       DAFCLG010000121         7250 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFCLG010000121 DAFCLG010000000
VERSION     DAFCLG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.122"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="LND56_002353"
     CDS             99..746
                     /locus_tag="LND56_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6744227.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="LND56_002354"
     CDS             1139..3784
                     /locus_tag="LND56_002354"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK6744228.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="LND56_002355"
     CDS             4094..5407
                     /locus_tag="LND56_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK6744229.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="LND56_002356"
     CDS             5397..6050
                     /locus_tag="LND56_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6744230.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="LND56_002357"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="LND56_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK6744231.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="LND56_002358"
     CDS             6947..7147
                     /locus_tag="LND56_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6744232.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2395 a         1229 c         1593 g         2033 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt
//
LOCUS       DAFCLG010000122         7228 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFCLG010000122 DAFCLG010000000
VERSION     DAFCLG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.123"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1332
                     /locus_tag="LND56_002359"
     CDS             <1..1332
                     /locus_tag="LND56_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744233.1"
                     /translation="AKRIGSSESTFVRYIEEIKIRWGTDVTIQSSQKLGYRLEHLNVP
                     AYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKISISRSSFTRKLKQCNQILESY
                     LLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTLPFPIEKKKIKEIMVKNNCQM
                     NLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLG
                     KLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQ
                     LLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIY
                     YLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTA
                     ITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(1405..2052)
                     /locus_tag="LND56_002360"
     CDS             complement(1405..2052)
                     /locus_tag="LND56_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6744234.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2060..2746)
                     /locus_tag="LND56_002361"
     CDS             complement(2060..2746)
                     /locus_tag="LND56_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6744235.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2752..3825)
                     /locus_tag="LND56_002362"
     CDS             complement(2752..3825)
                     /locus_tag="LND56_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6744236.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4047..4721
                     /locus_tag="LND56_002363"
     CDS             4047..4721
                     /locus_tag="LND56_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6744237.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4726..6066
                     /locus_tag="LND56_002364"
     CDS             4726..6066
                     /locus_tag="LND56_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6744238.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6215..7201)
                     /locus_tag="LND56_002365"
     CDS             complement(6215..7201)
                     /locus_tag="LND56_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBK6744239.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2230 a         1356 c         1267 g         2375 t
ORIGIN      
        1 gccaaacgaa ttggtagttc agaatctaca tttgtaaggt atatcgaaga gatcaaaatc
       61 cgctggggaa cggatgtaac gattcaatct tcacaaaaat tggggtatcg tcttgaacat
      121 ttgaatgtac cagcttattt aaatgttctg atggaaatgt cgcaaagttc tacaaacagt
      181 cgtttattaa atgagattat tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa
      241 atttccatca gccggtcttc ttttacacgt aagttgaaac aatgcaatca aattttagaa
      301 agctatttat taaaagtgat cgttgaccaa ggttttcagt taactagcct taaaagtgag
      361 ctccagttgc gtatctttgt tacctttttc tttttaagtt actacggaag acgcacgttg
      421 ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg
      481 aatttgtttc aggaagaaag cccatatgag caatctttct ttattatgta ttttgttgtg
      541 catctcatga gagaatccca aggaactcca tttgaatgga aagctggaga ctcttatgta
      601 cagttgataa atatcgataa aaaagacttc ttaatcttga aaaatctctt aggaaaattg
      661 aagtacagca gctatataaa agtagtgaac tattttgtca atacgatttt ttcagaattt
      721 tcggagaaag aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc ctactgttcg
      781 ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa
      841 atttattttt tctcccgtat tttcccatac gatacgactc atttgacgat ccgtgcaact
      901 tactttgcac agcagatgca acaccgccag atacgtctat atgctggatt tcgtgcagaa
      961 cttcaatttt attccactct actgaatcag aatctgattt attatttatc tagctatatc
     1021 ttttggatgc ttatcggtaa tcctgaaatt caccattata cttctgataa aaaaatactg
     1081 attatcagcg acttaagtct tcgacatagt caatatattg aagaatatat cagtgatatt
     1141 cttgctgtgc ataaaattca ctctgagaca actgctatta ctgaagatca gctgtcgaaa
     1201 tacaatcttc tcgagtatga tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt
     1261 cctcatatat tgattgatga tagtgtgtca ttcgcaaatg aagaagaact catccgctta
     1321 ttcgagttat gaataatcaa aaaacagcta tgtataaacc accctgataa aaatttcagg
     1381 acaaatacac atagctgttt ctttttattg tattccttca gcaatccaac gagatttata
     1441 gcgaaagtct tttaacaact cttcttttgt ccaattattt gcaatgagat ccgtcaaagt
     1501 gttcattacg agggtcatgg aaaagtatag gaaactaact gcttcttccg acgattgaat
     1561 ggagaaatga ttgattctta ataattccag ccatttttcg ttcattggag aatcagccga
     1621 tagttctcta cgtttggttg tctgctgatc atttcgctgg gttaacaagt ataacctttg
     1681 ccaatagata ctttcgtgat ctaagtcgca gcaccactct aaatattttt cgatccccaa
     1741 aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata tcttgatgga ctatgtgaga
     1801 actgcttttg atcgtataca aataagcatc ttccaaatct tgaaaatatt tataaaaagc
     1861 accgcgcgac atccccatct tgtcaacgat ttctgagact ttgacttggc tgatgtgctg
     1921 tgagcaaaat atctccagta ataggtcatc gattctctgt ttctttgcgt tcgataaatt
     1981 gaaaaaagtt tcttttggca tccacatctc tcctttatct ctgattatac gtggatagcc
     2041 cttcattgtc atgaaggatt cattccttct ttttctcttc ttctgtcaag tacccatctt
     2101 ccattcgata aacgcgatca ctccattgaa tcatacggac atcatgagta accataatag
     2161 tcgctttttg tttctcatga gcttctttaa ctagtaattt gacgacttca tatgcatgat
     2221 ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa gcttggctca ttatataaag
     2281 cgcgagcaat tgccactctc tgtctttctc caccagaaag gtctttagga taactatttt
     2341 taagtgactg gatatctaaa gattgcagca aggaatcgat ttttgttgaa tcttcttttt
     2401 ttgaagtaac ttggtcaact agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat
     2461 tagagctttg gagaataaat cctatttctt taaagcgtaa atctgctcgt tttttttctg
     2521 gaagctcagt tagtgagtga ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc
     2581 ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg atgctgacga
     2641 attctccttc ctttacataa aaatcaattg ctttcaaagc tgagaccttt gtatgaccag
     2701 aaccaaatgt tttggtaact tttttcatcg ttaatatttt tttcattttt attaacctcc
     2761 tattgctgta attgggtcta ctttggttat cgtacgtatc gaaaacaatc cgcctagcat
     2821 tgcgacaatg atcaaaatcc cactatatag caaccattgt gaccaaaaaa cagcaaaagg
     2881 catcgcgctt ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc
     2941 taccacccca acaataaagg actgtgctac taaagattta gcgatgaaga aatttttgat
     3001 cccttgtgct ttcattactc caaaaatagc agtcttctga agtgtcatca cgtacatgaa
     3061 aataccgacc acggcagcaa ccactaagaa caaaaagtat atcatcgcat ttaaggtcat
     3121 attttgggca gaatagccag gaatactttc aatgaatgtc ccaatagtta ataactgtag
     3181 acccttcgat gctttttctt tcgaaatttt agctgaatcc tttattacga tcccattgat
     3241 cggctgttct tcttcagaag caaacggctg ttgtccatac ttcaaagcag tccacgtatc
     3301 tagggacgca tagatgacgg gagaaactgt gtaataagtt tcaggaaaga taccgacaat
     3361 cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct ccaattttat agcctgaatc
     3421 agccagattt tgagagatga tgatttcatt ctttgtttta aattcatgcc ctttcgtgag
     3481 ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt ctttgttttt
     3541 tcctttgatt gctccgctgt acaatccgat tggtgctttc tctcctcctt caatgtattt
     3601 cagatctcct cttgttaatt gtgaagctgc aaatacttga tttgcatcct ccgacaaaac
     3661 aatctcttgt gcgtcccaat catcgattgc ttttttaaac tcttctgaaa gtccattcgc
     3721 tagtcctgac aacatgaata cgacatacgc aatcaagaac ataataccga cgatcaagcc
     3781 atatcgtaat ttagaatagc gaatttcttt gatagctaga aacatttgtt tttcctccct
     3841 atgatagatt gatagtgaca aaatgtcact tgttgaatag tgtaactcta aagtgacaat
     3901 ttgtcacttg tgttgttttt ttcttatcaa taaattgtct aagacatctt tcgataaaac
     3961 cttaacatga gtcataaaaa atttatttaa gccttattta tgctttccat gataaactaa
     4021 agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt agtagtagaa gacgataaag
     4081 tattatccaa taatataaaa gaaatactcg ctgcaatcgg cgagataaca caagtttatg
     4141 atggtagtga aggactgttc gagttggaaa gtggagttta tgatcttgca atcttggatt
     4201 tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac
     4261 agactccagt attgattttg actgcaaaag atagtttgga agataagatt gaaggatttg
     4321 aacgaggtgc agatgattat ctaacaaaac ctttctatcg agaagaactg atcatgcgcg
     4381 tgaaagcatt attgaaacgc tcgctaggtc tctttgatga aaatgttctg aagtataagg
     4441 atatcagttg tgatttaaaa agcaatgaag tcttgtataa agaacaagtt ttgcccatac
     4501 aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa taagggaatc attttgacaa
     4561 aagagcagat atttgaccgg atctggggat ttgattctga tacgacgatt acagttattg
     4621 aagtttatat gagcaactta cgaaaacatc tgcgaagtac tggattggat aaagaaatca
     4681 aaaccttacg taatgtcggt tatatactgc aaagggtgtg atgcaatgaa tggagatatt
     4741 acacgaaagc aacgtagtcg cttttttgcg acaaatgtag cagcatttgc ttttatcttt
     4801 tttcttttgg gattcattac tttacagata ttaaaccaat ctgcatacag agaaacagat
     4861 ctgtcattag agaaaataaa ttttgattct cgaacagtac aattagagat acaaagatat
     4921 cagcaaggcg atcccttttt aaacccgact caaacggaac aaaacagaac aaacatggaa
     4981 cagccattaa tggaaccatc aaatgaccgt tttaacactc aagtgatctt gtggtctaaa
     5041 aatggtgaga tattgaataa agcagctatt ggtgggcgtt tcaaccaaat caaagaatta
     5101 aaaatggata cgaaaaatct gaataccatc caaaatattg aattaaatga agacagtggc
     5161 tcatctgccc tctctttccg ttcgattacg aaaaaagcgg agacgaatca agatggcgtt
     5221 gcctacatcc aaattttaga aaatacaaat caaatcaaga attcattagc gacttttcgc
     5281 acgattatga ttttgtgtat gattgttttt tggattctct ctattggtat cagttattac
     5341 ttgtctagtc taagcatgcg ccctattatt ctttcttgga gaagacaaaa agaatttgtt
     5401 gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag tttggaacat
     5461 ctttttacca agcctaatca tactattatt gaagaatccg aaagcatcgc tcaagcattg
     5521 tcagagacga ggcgcctcac tggattgact tcggatttgc tgacaatcgc tcggaatgat
     5581 tctgatcagc agctgctttc aaagcaaatg atcaatacac aagaatatat cgaacaactt
     5641 gttaaaccat ttcaagagat ggccatcata gatggaaaag agtttattct ccataacgat
     5701 gcaacattag aagtaccaat tgatgaaaaa aaaatccatc aagttttggt tattttgctg
     5761 gataatgcat taaaatatac gaatccagaa gataagatca ttttggattc atctgttaac
     5821 cataaagaat ggctgattca cgtaaagaat actggccctt ctatcagtga agaagatcaa
     5881 gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga gactggcgga
     5941 tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg acagatttct
     6001 gtatccaatt atcttccaca aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa
     6061 aaataatgct tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt
     6121 tgcgaataaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttctttaagc
     6181 tgaccacttc tgtcacaacc tctttagctt acgcttacga ttttttattg cggtcttcaa
     6241 tgtgtacatc accgcttgtg tcaatatcca attcttctcg gctaacatct tctgtcactt
     6301 tttcaacatc ttcatgttct tctttatgaa tcccaacttc ttcacgaata actgtatgtt
     6361 tgcttacatc tacttgctct tctttgattg ggatagtaat cgtgtcatct tccaattttt
     6421 cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg atttcttcac
     6481 gtttgaccgg tacttctacc gtttcagtat cgttgaccac atgtttgtgg atgtcgactt
     6541 ctccagttgt cacatcatgt gtatttacat ctaatttttc ttcttttagt tgaattgtat
     6601 catcatctag atttggtttg cgatcgatgt cttcagtctt gcttgtaccc attgtatcag
     6661 tctcgccagg tacgtccatc ggatcagttc ctggtgtcat attcaatgga tccgttgtat
     6721 tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca gtttctaatc
     6781 cagtccccat tgtgcttgct gggctaacag tatcttgtgt atccattgta tcttctgttg
     6841 tttctggctg acgatagcct tttactgtga tgacaaattt tccatcagaa atatctcctc
     6901 tatattgata taaaggatca ttttctggtg tagccgtctc agaatcatag gtatcaaaag
     6961 aaaaagtatc ttttattttt tcccataaag aacggtcttc ttcaccatcc atccgatttt
     7021 catctgtttc aacatctata ccagacaatc cgtcaaatcc tagtctagat gctgcttcat
     7081 cagtagtata aataacgata tcatctcgtt catacccttc gttacgcaac cgttcaatta
     7141 ctgataatgc ttcttccctc gtactatagc ttccgatgac cgttaaattt ttctcttcca
     7201 tgtaaattcc tcctagcaaa tagattta
//
LOCUS       DAFCLG010000123         7170 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFCLG010000123 DAFCLG010000000
VERSION     DAFCLG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.124"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..1248
                     /locus_tag="LND56_002366"
     CDS             join(107..335,335..1248)
                     /locus_tag="LND56_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6744240.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="LND56_002367"
     CDS             1483..1935
                     /locus_tag="LND56_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744241.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="LND56_002368"
     CDS             1949..2638
                     /locus_tag="LND56_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744242.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="LND56_002369"
     CDS             2763..5114
                     /locus_tag="LND56_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBK6744243.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="LND56_002370"
     CDS             5194..5589
                     /locus_tag="LND56_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744244.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="LND56_002371"
     CDS             5914..6531
                     /locus_tag="LND56_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744245.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="LND56_002372"
     CDS             6572..6871
                     /locus_tag="LND56_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBK6744246.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="LND56_002373"
     CDS             6905..7072
                     /locus_tag="LND56_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6744247.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT         2494 a         1062 c         1445 g         2169 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt
//
LOCUS       DAFCLG010000124         6802 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFCLG010000124 DAFCLG010000000
VERSION     DAFCLG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.125"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            165..1037
                     /locus_tag="LND56_002374"
     CDS             165..1037
                     /locus_tag="LND56_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6744248.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1034..2992
                     /locus_tag="LND56_002375"
     CDS             1034..2992
                     /locus_tag="LND56_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6744249.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3243..4094)
                     /locus_tag="LND56_002376"
     CDS             complement(3243..4094)
                     /locus_tag="LND56_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6744250.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4217..4684
                     /locus_tag="LND56_002377"
     CDS             4217..4684
                     /locus_tag="LND56_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK6744251.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(4947..5429)
                     /locus_tag="LND56_002378"
     CDS             complement(4947..5429)
                     /locus_tag="LND56_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK6744252.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5529..6392)
                     /locus_tag="LND56_002379"
     CDS             complement(5529..6392)
                     /locus_tag="LND56_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6744253.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2029 a         1407 c         1237 g         2129 t
ORIGIN      
        1 gattacgtta acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct
       61 gaggaacatg gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact
      121 ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga
      181 tctttaatca aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa
      241 aagggattgg atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga
      301 tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg
      361 aacgcttttt atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa
      421 agttgaatga acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca
      481 ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg
      541 atgaattcag tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat
      601 atacttatga cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga
      661 acaataacca tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg
      721 aatcagaatt ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg
      781 tgaatcattt acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca
      841 cggaaatcgt agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa
      901 tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact
      961 tggccatcca tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg
     1021 aggaggaaaa aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt
     1081 tgcggtctta cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag
     1141 taacgacatt gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc
     1201 tttgctgttt gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc
     1261 cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc
     1321 tactttattg aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt
     1381 atttattggt attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc
     1441 aaaattaccg atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc
     1501 ctttgtcatg ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc
     1561 tttagtttct tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg
     1621 cttcttcaac cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg
     1681 gtttgacgta gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg
     1741 cgtaacagga atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg
     1801 agcatatgct atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat
     1861 gctagcagca gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt
     1921 tatgttcgtt gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc
     1981 agtgagtgca ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta
     2041 cttcctatct ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt
     2101 agtaacagca gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat
     2161 gactcctggc cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga
     2221 caataaatat gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt
     2281 aacttcgatc gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat
     2341 cgatcaaaat aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa
     2401 tgcgcaagtc atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca
     2461 tggtggtgct gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc
     2521 cggtgaaacg atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac
     2581 tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc
     2641 agaaaatgga gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa
     2701 acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa
     2761 cactgttgac ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc
     2821 aagaggtcaa ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac
     2881 ggatatggtc gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc
     2941 acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt
     3001 cttccccaga cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc
     3061 tcctttttcc gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa
     3121 acgaaacata tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc
     3181 tttttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
     3241 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
     3301 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
     3361 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
     3421 aacgctgcat cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc
     3481 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
     3541 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
     3601 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
     3661 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
     3721 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
     3781 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
     3841 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
     3901 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
     3961 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
     4021 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
     4081 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
     4141 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
     4201 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
     4261 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
     4321 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
     4381 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
     4441 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
     4501 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
     4561 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
     4621 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
     4681 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
     4741 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
     4801 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
     4861 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
     4921 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
     4981 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
     5041 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
     5101 aattcttcag gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg
     5161 atgattcgcg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
     5221 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
     5281 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
     5341 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
     5401 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
     5461 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
     5521 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
     5581 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa
     5641 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
     5701 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
     5761 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
     5821 cggtctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
     5881 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
     5941 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
     6001 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
     6061 cgtggaaaag atttttgtat gccgtatcta ctgatttctg aaggcagata tcctgaccaa
     6121 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
     6181 gtcactcttg atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata
     6241 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
     6301 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
     6361 taatcgatca attggttcag atctttaagc atatttatct tcctccttga ttgccttttt
     6421 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
     6481 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
     6541 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta
     6601 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
     6661 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
     6721 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
     6781 tttgtcagat aaataaaaaa ca
//
LOCUS       DAFCLG010000125         6775 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFCLG010000125 DAFCLG010000000
VERSION     DAFCLG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.126"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            254..739
                     /locus_tag="LND56_002380"
     CDS             254..739
                     /locus_tag="LND56_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBK6744254.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            732..1415
                     /locus_tag="LND56_002381"
     CDS             732..1415
                     /locus_tag="LND56_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK6744255.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1541..4186
                     /gene="polA"
                     /locus_tag="LND56_002382"
     CDS             1541..4186
                     /gene="polA"
                     /locus_tag="LND56_002382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK6744256.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4231..5067
                     /gene="mutM"
                     /locus_tag="LND56_002383"
     CDS             4231..5067
                     /gene="mutM"
                     /locus_tag="LND56_002383"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBK6744257.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5031..5660
                     /locus_tag="LND56_002384"
     CDS             5031..5660
                     /locus_tag="LND56_002384"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK6744258.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5712..6044
                     /locus_tag="LND56_002385"
     CDS             5712..6044
                     /locus_tag="LND56_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6744259.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6356..>6775
                     /gene="nrdR"
                     /locus_tag="LND56_002386"
     CDS             6356..>6775
                     /gene="nrdR"
                     /locus_tag="LND56_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBK6744260.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVF"
BASE COUNT         2285 a         1200 c         1496 g         1794 t
ORIGIN      
        1 cttgccaagc cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg
       61 gtaataagcc caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta
      121 tttgatgcag gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg
      181 gagcagaact tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg
      241 gagggtttct atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat
      301 cgaggaaggc gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta
      361 tttgggattg acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac
      421 agaatatcaa cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat
      481 ttcaaagcat cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga
      541 accggttttc cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat
      601 gaaggatgtg gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga
      661 tgctgtcgtt atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga
      721 aggtgaattg aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa
      781 aagtttatgg atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc
      841 tttgggtatt tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg
      901 gtctgtggat cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc
      961 cgagcttagc aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg
     1021 ttactcttgc atactacgat attggaacag taacgcaagc tttcttgaca gctactttga
     1081 tgttcatggg gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa
     1141 gagcaggaca ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt
     1201 tgagaagtag cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga
     1261 ttacagctta tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac
     1321 atagtggaat tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct
     1381 tggcgtttct gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg
     1441 gctataccga attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt
     1501 gttagaatga ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt
     1561 actcgttgat gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga
     1621 acgtttcaaa aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt
     1681 tgaaaatgtt atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa
     1741 aacgactttt cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg
     1801 cgaattcaaa gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta
     1861 ttacgaattg cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga
     1921 caaagatcaa tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag
     1981 taaagaagta aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc
     2041 agaacatgta gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg
     2101 actagccgga gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc
     2161 tatcaagcta ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat
     2221 gaagcaaagc aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa
     2281 aaaattggcg accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta
     2341 tgaagggaaa aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt
     2401 cctttctaaa ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt
     2461 cgttcatgaa ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat
     2521 gggcgacaat tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat
     2581 ttatgtcacg aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga
     2641 tccgactcgg ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta
     2701 tactggaaaa acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac
     2761 gaatgaaaaa agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca
     2821 atcagatgaa gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact
     2881 cttttttgct catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag
     2941 ccaagaactg gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc
     3001 tatcattttg gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga
     3061 aatgaagggc gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc
     3121 cggcgaagaa ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat
     3181 gggattaccc gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga
     3241 acagcttcgt gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa
     3301 aattcaatct acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca
     3361 tacccgttat gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt
     3421 gcagaacatc ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag
     3481 agaaaaagac tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc
     3541 gcatatctca aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc
     3601 cagcacagcg atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg
     3661 taggcaggca aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc
     3721 acaaaatcta ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa
     3781 atacccaggt gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta
     3841 cgtcgaaacg ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa
     3901 tatccgttct tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga
     3961 tattttgaaa atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc
     4021 tacgatgctg ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga
     4081 atcattagat aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact
     4141 gatcactgac agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac
     4201 agagcaatat aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt
     4261 cgtaaaggat tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg
     4321 cccaagatca tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc
     4381 atccaatcca ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg
     4441 atctcacact tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat
     4501 aaacatacgc atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta
     4561 cgaaaatttg gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc
     4621 atgaaattgg gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga
     4681 ctgaaaaagt cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt
     4741 ctaggtaata tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg
     4801 gcgaacacac tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg
     4861 gatcgtgcaa tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa
     4921 tcaggcggat ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt
     4981 tgtggaacgc cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat
     5041 tgtcaaaggc taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta
     5101 ccggtaaaag tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg
     5161 atgtgattgc cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag
     5221 tgtttggcca agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga
     5281 ttgtttttcc atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc
     5341 gaaaagcgat aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag
     5401 atatcccatt gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt
     5461 atactacgcc agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac
     5521 aagcgcagca gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata
     5581 ttttgttcga caacaatgga acaaaagaaa aacttgtggg acaaatcgaa aattggcttt
     5641 ttgctaatcg ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt
     5701 gagggatagc gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag
     5761 aaaaaatcgg cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag
     5821 aaacagcaga agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc
     5881 aggcagaaga agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg
     5941 atcgtagtaa aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg
     6001 aacagcgaat gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga
     6061 gatgtgactt gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga
     6121 ctaaatcagc ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt
     6181 gaaatttagt aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata
     6241 tccgtgtttt ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta
     6301 gttgtaaaga aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag
     6361 atgtcccaga tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg
     6421 acgtgcgatc cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga
     6481 acgaattgaa gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa
     6541 tcgcgataaa attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga
     6601 acaaatggaa caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga
     6661 agtatcgacg aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat
     6721 cgcttatatt cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttc
//
LOCUS       DAFCLG010000126         6520 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFCLG010000126 DAFCLG010000000
VERSION     DAFCLG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.127"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(195..773)
                     /gene="pgsA"
                     /locus_tag="LND56_002387"
     CDS             complement(195..773)
                     /gene="pgsA"
                     /locus_tag="LND56_002387"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK6744261.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(982..1905)
                     /locus_tag="LND56_002388"
     CDS             complement(982..1905)
                     /locus_tag="LND56_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744262.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(2644..3930)
                     /locus_tag="LND56_002389"
     CDS             complement(2644..3930)
                     /locus_tag="LND56_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6744263.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3923..5191)
                     /locus_tag="LND56_002390"
     CDS             complement(3923..5191)
                     /locus_tag="LND56_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6744264.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5443..5904
                     /gene="mscL"
                     /locus_tag="LND56_002391"
     CDS             5443..5904
                     /gene="mscL"
                     /locus_tag="LND56_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK6744265.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5963..6409)
                     /locus_tag="LND56_002392"
     CDS             complement(5963..6409)
                     /locus_tag="LND56_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6744266.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1837 a         1381 c         1158 g         2144 t
ORIGIN      
        1 tttatttaaa aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa
       61 ataaacagtg ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa
      121 aagctgtttt ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct
      181 ccactaaacg atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc
      241 acacctgaat agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata
      301 ccgatcaggc tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta
      361 gccgtcttga cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt
      421 aaacgcaagc ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg
      481 gcctttcctt ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac
      541 gggtccgcaa acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct
      601 aaccagtctg tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc
      661 agtgtggtgt ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg
      721 aaaatcggga tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg
      781 actcctttct tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt
      841 ttccaaatga atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga
      901 agaaggaccg cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga
      961 tgcgtaaact aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg
     1021 actctgattg ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc
     1081 attgcttgct gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact
     1141 ctgtgtttca tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc
     1201 taaccataca cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat
     1261 atttactgcg atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc
     1321 acttgatttt gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga
     1381 ggaagaggac tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat
     1441 tggatcagct tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc
     1501 aaccaatcct aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc
     1561 ctgatgcatt gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga
     1621 tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac
     1681 agcttcagcg tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa
     1741 atcatcattg tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc
     1801 atctaaagat atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa
     1861 agccactcgt tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga
     1921 aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa
     1981 acattgttct cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc
     2041 tgttttttga acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct
     2101 taaacggtgt tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga
     2161 gatatttttc cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc
     2221 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt
     2281 aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     2341 tcttggtaca cggtgttcca gaagtagctt cttcggcaat aagccgaaat atcacaaaaa
     2401 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca
     2461 acctctttga ttacttcgtt tgattttatt ttaatataag ttgattaaaa gagagcatga
     2521 tttgttttat tttctcacta atttttccaa cacaactaag aataccataa aagctgattt
     2581 tggaggaggt tcctactaca agtctaaaac gaaatttaag acttttcctc ataaaatact
     2641 ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa atgcttcccc
     2701 agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt
     2761 tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga
     2821 ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc
     2881 cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc
     2941 gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc
     3001 aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc
     3061 taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc
     3121 ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc
     3181 ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc
     3241 ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc
     3301 aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata
     3361 gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc
     3421 gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt
     3481 agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt
     3541 ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac
     3601 ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga
     3661 agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg
     3721 ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat
     3781 aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc
     3841 atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc
     3901 attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg
     3961 cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac
     4021 gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag
     4081 cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt
     4141 ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt
     4201 gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga
     4261 atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct ttctctctga
     4321 cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg
     4381 caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt
     4441 gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa
     4501 acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg
     4561 ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt
     4621 cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca
     4681 accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag
     4741 caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca
     4801 agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt
     4861 ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa
     4921 aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc
     4981 ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgga taattcaaac
     5041 tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac
     5101 gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa
     5161 gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg
     5221 cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt
     5281 ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag
     5341 atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt
     5401 tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa
     5461 gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg
     5521 tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt
     5581 gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg
     5641 ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg
     5701 atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc
     5761 tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac
     5821 atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca
     5881 aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg
     5941 tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg
     6001 gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag
     6061 cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca
     6121 tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa
     6181 caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga
     6241 tcttcccatc agtatcttga aaaaacgtat gccaagcttg tccatcaatg tcatcaactt
     6301 cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt
     6361 aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc
     6421 cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg
     6481 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa
//
LOCUS       DAFCLG010000127         6374 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFCLG010000127 DAFCLG010000000
VERSION     DAFCLG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.128"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..698
                     /locus_tag="LND56_002393"
     CDS             <1..698
                     /locus_tag="LND56_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK6744267.1"
                     /translation="GVGAVAMMITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVD
                     GPLSNSTYIESFQKVWNRHKELSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEEN
                     QERLMARYEESIRYSRRIGNLYTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSE
                     FFTGYLEENYQEYLFAQSHQEMLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFS
                     ITKIENDIRYYKI"
     gene            complement(720..1472)
                     /locus_tag="LND56_002394"
     CDS             complement(720..1472)
                     /locus_tag="LND56_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6744268.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1836..4502)
                     /locus_tag="LND56_002395"
     CDS             complement(1836..4502)
                     /locus_tag="LND56_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6744269.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNFPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4843..6294
                     /gene="cls"
                     /locus_tag="LND56_002396"
     CDS             4843..6294
                     /gene="cls"
                     /locus_tag="LND56_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6744270.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1867 a         1326 c         1136 g         2045 t
ORIGIN      
        1 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
       61 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      121 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      181 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      241 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      301 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      361 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      421 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      481 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
      541 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
      601 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
      661 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
      721 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
      781 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
      841 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
      901 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
      961 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1021 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1081 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1141 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1201 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1261 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1321 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1381 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1441 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1501 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     1561 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     1621 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     1681 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     1741 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     1801 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     1861 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     1921 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     1981 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2041 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2101 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2161 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2221 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2281 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2341 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2401 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2461 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     2521 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     2581 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     2641 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     2701 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     2761 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     2821 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     2881 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     2941 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3001 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3061 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3121 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3181 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggaagtt gtttcggatt
     3241 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3301 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3361 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3421 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3481 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     3541 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     3601 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     3661 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     3721 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     3781 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     3841 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     3901 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     3961 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4021 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4081 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4141 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4201 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4261 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4321 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4381 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4441 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4501 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     4561 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     4621 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     4681 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     4741 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     4801 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     4861 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     4921 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     4981 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5041 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5101 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5161 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5221 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5281 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5341 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5401 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5461 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     5521 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     5581 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     5641 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     5701 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     5761 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     5821 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     5881 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     5941 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6001 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6061 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6121 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6181 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6241 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6301 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6361 tcccttcttc cgaa
//
LOCUS       DAFCLG010000128         6208 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFCLG010000128 DAFCLG010000000
VERSION     DAFCLG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.130"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..1185)
                     /locus_tag="LND56_002397"
     CDS             complement(55..1185)
                     /locus_tag="LND56_002397"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK6744271.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1245..1472)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1834..3036)
                     /locus_tag="LND56_002398"
     CDS             complement(1834..3036)
                     /locus_tag="LND56_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6744272.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3096..3299)
                     /locus_tag="LND56_002399"
     CDS             complement(3096..3299)
                     /locus_tag="LND56_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBK6744273.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3441..4673)
                     /locus_tag="LND56_002400"
                     /pseudo
     CDS             complement(3441..4673)
                     /locus_tag="LND56_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4836..5648
                     /locus_tag="LND56_002401"
     CDS             4836..5648
                     /locus_tag="LND56_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744274.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1827 a         1281 c          990 g         2110 t
ORIGIN      
        1 actccatgaa aagccgactt tttgggacgc ttttttttgc caagcctcac atttttatag
       61 ccaaacttct tttgcgactt gctgcaataa ttggattttc ttccattgtt cttcttctgt
      121 taaatggttt ccttcttctg ttgaagcaaa cccacattga ggactgatac ttagattttc
      181 caatggtaca aattgttgtg cttcttttat tcgttctttt attttctccg tatcttccaa
      241 atctggaaac ttagaagtaa ctaatcctaa aacaacttta ctttctttgt tactccaatg
      301 tcttaaagga gtaaaatctc ctgctcggtc agaatcgtat tccaaaaaga aaccatcata
      361 atttgttgaa aacaactctt ctgcaatggg ttcatatcca ccggaaaaca gccaagaaga
      421 agcgtgattc ccgcggcaga tatgtgttgt gatcgtcaaa tcttctggtt tgtcttccag
      481 tgcgttattg ataatctctg tacaaatatg ggccatttct ttcggatcaa ttcctttttg
      541 aagaagtgct tctcttttag tttgatcagc tagaaacgcc caatttgtat catcgaattg
      601 caggtaacgg caaccttttt cataaaaagc atgaatcgct gctttgtaag tagaggacaa
      661 gtcctcataa aaatcagata gatcaggata aatctccttg atttttgaag aaccatcatt
      721 agataggatc tcttgtcgta aaatcatcgt gggactaggg atcgttgctt ttgcaacaaa
      781 agtatcatct gtttccacta cttctttcag aaaagcaaaa tcttttaaaa atgggtgatt
      841 tggattgaaa gataatttat ctactacccg aatattataa gctggtgctg cttttccgtg
      901 gaatgtttga ttatatccag tttctggaac atagccttcg ataccattta gttcttctaa
      961 aaaatcaaga tgccaaaatc cacgccgaaa ttcaccatct gtaattcctt tgatgccgac
     1021 tgctttttgg tcttccacta atttttttat ttcctggtct tcgattgcac gaagttcttc
     1081 ttttgaaagt aagccttctt gaactttttt tcttgcttct ttgatcgatt gcgggcgtaa
     1141 gaaacttccg acatgatcta ctgtaaatgg tctatttttg ctcatttcac tcactctttt
     1201 ctttgttttt tgtaaaaaaa aatcctttta ctagcaaata ctagtaaaag gacgattata
     1261 ctcgtggtac cacctttttt cagaaaaaac attctttctc tcagcctgac aataaaatca
     1321 gtggtcttgg taacgaaaga ctgttcgttt ttgcctacct ttcgacaaaa aatcctctaa
     1381 ggccattttc gacagtcaac ttctagcttt ttacaccaac caaagcctct cttgaaccat
     1441 tttctgtcta ctttccttct catcggatac tattagaata aaattagaat agcatgtttg
     1501 aaagaaaagt caatagggaa taagaacaaa caaaaactaa tttgttattc ttagtaattt
     1561 gtttgtggaa tacaataaag cttttctaat atgataattg ttcagtcgtc ctttcatctt
     1621 agatcacttc cttaaaatga tcatacccgt ttcaaccaca taaaaaagga cttaattttg
     1681 aaaaatttcc catcaaaact aagtcctttt tattatagtt atgcaaattt gcttattttc
     1741 tcgctatttc cgatacatct tgaacaggag caattcgact gaaaacactg gtgcaaaagc
     1801 atttatttgt cttatggtag aaccgtggaa aactcagact ggtaataaat tgtcattttt
     1861 tgaaagaata gcgccctctt gaacattatc aatcgcactt accacttgtt gatctgactt
     1921 tgcatataag tgaccgtaag ttcctaaagt gatttgaata ttcgcatggc ccaatcgttc
     1981 ttgaatagat agcggttgaa tattctgctc aattagaaat gcaacatgag aatgtcgtag
     2041 cgcgtgaagc ttaatcggtt tgacacccgc ttttttagca gcttctttta accagttcgc
     2101 aaaagaataa ggtggcgtaa atgttccgtc caactgagca acatattcaa aatttccaat
     2161 acgtttttgc aagtcatacc actgtctgag ttttaaggaa agcttcttcc ccaacccaat
     2221 cgttcgataa gcacatttag ttttgggagt cgaaaagtac caatcatctt ttgtattata
     2281 aatcaatgtc ttctcaatcg ttatcgtgtt tttctcaaaa tctacattcg accaagacaa
     2341 agcttcaagt tctccaatcc gaatgccagt ataaaagagc atttcataca caatcttccg
     2401 atggtgggtt tgaatcttat catccttcaa ttcattcatc actttttgaa actcactgtg
     2461 ttcccaaaac tcaactttct tattctcaga aggaagcgaa gaaacatctt caacgggatt
     2521 tttagcaatc accccctctt taacagcccg atccaataca atttttaggt gccctcgaat
     2581 ccgattgata taagattttg ataacgtttc gccattaact tttttccgtg aattacgtga
     2641 ctctgtctgg cttagttcaa ataaccaatc ctcaatatcg tgacttgtaa tatctcgaac
     2701 acgaaaatga tagaaaaact gcatacgctt ttcgaaaatg aattgcgcat tctcatatgt
     2761 ttgacttttg acctgtctct cgtaccattt tacaaaccta tccttgtaaa actcttcgaa
     2821 agtcatttga ttggtagaac acagttgatt aggaattgtc ttgttaaaat catgtgtttc
     2881 catgtacttg tcataagcaa ttttggcatc tttttgtgaa gcataacctc gtttagtctt
     2941 ctgaaccttt cttccatttt catctatacc tagatacgct cggaagaacc atttttttgt
     3001 ctttttatct tggtaaatat ttttaatttt tggcatcgtt ccattcctcc ctgtacttga
     3061 gaaaagctac tttaatagta ccgcgcttgt actgtttatt ccgcaacttg aatgtgaaaa
     3121 agatgttcaa ttactcggga tggcaccaca ccaacttggc gattattata aaaatcaata
     3181 ccttcaatcc ttgcaagata tgccttgctc tctttaatca ttcgtgcagc ttgactaggt
     3241 ttaaagccca tctctatcaa aattgcacga tttaccatga aatcacattt tgtttccatt
     3301 ctctcacctc ctgattcttt actcctttta aaataatcct ctttataatc tattgtttct
     3361 cattcgatgg ttgtgttata ctcgtcttgt cgaggcggag tacgttgcac aacttgttgt
     3421 gtaagcagta ctttgctttt ttagtagctt gttttctcat ttgatatact ttgtaatttt
     3481 cgttcataat actcaacctc ctcctgataa tactccgggt ctgccttcat attttggaga
     3541 agtttgatct gtcttggatt catttctcct tgttcaataa tcaaaggcaa caaggaagaa
     3601 tgaaacgttt gatcgacctg tgcaataaaa actaatgatg gtgaaacacc atgaattaac
     3661 caactcatag ttttctcaaa actttgagga attgtatcta aagaaatggt gattgcgcca
     3721 taattgctaa taaaggctgc ccatttggca tcaacttttg cacctagatc atttggatca
     3781 cggtcataaa aacaaattgc tccattaagt aactcaaaga acagtttggt taaatcatgg
     3841 gtttttgaaa taatctttgc caagctttgc gctttctctt ttcgataccg tagttcaaag
     3901 cgattcttta cttttgcctc ttctaaagga attcctcgtc gcttccgttg ttcatggtcc
     3961 ttctgataaa agcaaaaacg acactgactc ttctttgagc ctaaatacaa ggtctcacca
     4021 tttgattcat tgtttatgat atccgtaccg ccatgagatt cactcacttg aaaagtggtc
     4081 cacacatggc ctagtattac cttatgctta agttctggaa tgctgagact ccccacaaaa
     4141 tcatctaaag taaaatcaat ccgtgtaaag ttcccctgat aatctaatac cttacgaaaa
     4201 aactgttgcc aatttgtttt tcgtgttttt agccatcttg ctaagtgttt acagccttga
     4261 cctgaaaact ccatcacagt tcctttaacc gcatcgtcaa tggaataccg aatcgtgatt
     4321 acttgattcc acacgtattg gccaatatag ccctttggcg cataatccag ttcctcaaaa
     4381 cgttcaatgg gcatttcaat aatctcttca attagcttta aatgttgatt cgagaaaaag
     4441 tgaatggtta gataatcaat caaaacacgg ttaactggaa cgacctcttc ttgcaaacta
     4501 tccaacacaa gattcaattt ttcttcactt aattgtctcg ttcctttttc tactttcgat
     4561 aataactatc tcgaaatccc cactttttct gctaaatatc gttgtgtcca ccccctttct
     4621 tctcgagaaa ctcgaatggc ttcaccgaga tcatgttgat taacttggtt catcgacaaa
     4681 cctccttctg ctatttactt gtcaaagaac ctattgaact tattttaagt acatttttat
     4741 tgtacgcctg tatttatttt aagtcaatat gttttgactt tttttaagta cacacgtata
     4801 ctaaatttaa gaacttatcc tgaggaggag ttttcatgga gatagacaag caagcagtcg
     4861 gcaatcgaat tcggcagatt cgccaagaat taaagttgag tatggagaaa tttggcaaac
     4921 taattggtga tttaccgcga agtacagtca ataactggga acgtgggatc aaccttccaa
     4981 aaaccgaaac ccttcaccaa atcgcagaga taggtcatgt caccaatgaa tatcttttgt
     5041 atggtgatca agagaatcaa tacattttag aaatgctcca aaaaaaggca ggtaagcttg
     5101 atcctaagat tgaaggtctc atagtagacg aaatcaaaca cgctgacctt gttagtgaaa
     5161 aagaaatgga tcgaatgatt gaatttttta ttacaaatct cattccacca actgaacaag
     5221 accagtttac tttccaatgt atcgatgaaa aaaagcagct ttatcttggt tcaactaatt
     5281 ttggtaagga agcccagctc tatttgcaac atgataatca aaatcatatt ctgcacatga
     5341 tgccctttac cttctccaac tttagtgtgg atcgcatact cgtctattta gcaaatcaaa
     5401 aatcctattc ctattttggg aaacatttac ccaaggaact aggtgagaaa gccatccttc
     5461 tatactcaat taatcaattg accagcgatg tgcgaattgc cccgttggtc tattcagaag
     5521 aaaccgcttc ttatcagtac acagaggaca atcaatatct actagaacag caatatcttt
     5581 atctaccatt cgtgatggaa gtcgagaaag aacgcctgtt aaatgcagcg tatccatcat
     5641 caaaatgagg tgacaaaatt gataagcacg tgacaaagaa aatactgcta ggacactccc
     5701 caatgggtag ttgtcacagt ttcctgttct ttgttagccc cctgttagaa ccgggggctt
     5761 tattcctgca gtccggctac gccttcctgc aatagacaaa actccagatc gggtaattcg
     5821 cttgtggcga aacccgacct ggagtttgtc tctgtatttt gaaccctaaa aattaatcaa
     5881 acagactgat attctcataa tcagcgcgaa ataagccatt aagtacagtt ttcctaccta
     5941 tttttgataa cccgctggtt agttaatatt ccttttccga gagagataaa tgattggtta
     6001 aatctatata catctcaacc tttaaaaatg ttttcgaaat acttcatcag tacatgtcat
     6061 acaaattacc agtgcattct caaattctag ttctttaatt gatttcagtt gaggagcagt
     6121 tacttttggc ggaaatttcc atttttgtcc aaacctcttc ccccgatgaa atttctttgt
     6181 tgcaattatt gcaatgaaaa atttttgt
//
LOCUS       DAFCLG010000129         6205 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFCLG010000129 DAFCLG010000000
VERSION     DAFCLG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.131"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..694
                     /locus_tag="LND56_002402"
     CDS             <1..694
                     /locus_tag="LND56_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6744275.1"
                     /translation="GFNKKTGKNNPNFYYLLKPEFSEGDFYKKKCIEENLHLPTNQHY
                     SPSEKNTPSDKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKESQNADFIDSLSVENK
                     KLDEILIESLIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWYKKFKYAIKSAEKEQK
                     IQLKIIYEICNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALEL
                     IELQISEGIDKQ"
     gene            975..3668
                     /locus_tag="LND56_002403"
     CDS             975..3668
                     /locus_tag="LND56_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HBK6744276.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3682..3879
                     /locus_tag="LND56_002404"
     CDS             3682..3879
                     /locus_tag="LND56_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744277.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            3883..4494
                     /gene="mobC"
                     /locus_tag="LND56_002405"
     CDS             3883..4494
                     /gene="mobC"
                     /locus_tag="LND56_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBK6744278.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4479..5870
                     /locus_tag="LND56_002406"
     CDS             4479..5870
                     /locus_tag="LND56_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6744279.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
BASE COUNT         2354 a          857 c         1220 g         1774 t
ORIGIN      
        1 tggatttaac aaaaaaactg ggaagaataa tccaaatttt tattatttat tgaaaccaga
       61 attcagcgaa ggagatttct acaaaaaaaa gtgcattgag gaaaatttac accttccgac
      121 aaatcaacat tacagtccaa gtgaaaaaaa tacaccttcc gacaaatcaa cattcgatgg
      181 tgataagtca ttcccgcttc caggtgaaaa atttttacaa gaacttaatc attcttctaa
      241 agattttaaa gattttaaag aatctcaaaa tgctgacttt attgattcac tttcagttga
      301 aaataaaaaa ttagatgaaa tattaattga atcattaatt gaaaaagaat ggctttatca
      361 ggtatggggg gataatattg taaactgtct gaaattagtg ggtaataacg attacagtca
      421 attttatgac tggtataaaa aattcaaata tgcgattaaa tctgctgaaa aagaacaaaa
      481 aattcaactg aaaattatct atgaaatctg taataacaag tattttgtag attatgttag
      541 agaacagcta tcaatgactt tacgtgactt gatacgaaga gcaaaaacag atcaccgtat
      601 caaacaaaaa gacgattata tctttgcttc tctaaaaaat aaagcgttag aactaattga
      661 attgcaaatc agtgaaggaa tagataagca ataagaatag aattgagtca aatgaagtgg
      721 aggggtatct ccgcttcatt tgagaacttt gaatatagga ggggttattt tgattgaaaa
      781 tcgtttaata gatcaagttt ggtgtctaga tcaaaataca gataatctcg aattaatcga
      841 gactatttgc ttcaacacta tagttctcga tttaagatct gaaaatgaca attgtgtaac
      901 tcatatcata attgacagaa aaacagctca gaagctctct gagagtctca gaaaatgggc
      961 tgagggaggt aataatgaaa atagttaaag aaaagattgc cagtagaaaa atacactggt
     1021 tagctttgag catacttgtt ttaatgttta tcgttgtttt caccggtaat gttgtccaag
     1081 caagcatttt caattggttt gatgacacgg aaagtcaaac tgaatttttg aataatcctg
     1141 tctatcaatc gtatttaaaa aaatataacg gaaattttta cagtcagttt ggattgtgga
     1201 tagggtggtc tgttataaaa tcgatgtttg caattgtaga tggtattcaa aatatgattc
     1261 ccgaagtgtt agatttgttt agctttattg aaagtacggg aatgaataaa gtttatcaat
     1321 cggtcttaag tacagttgtt attggtttaa tgatactttc actgatgttt gttggttaca
     1381 aaatgattac tggaaaagga acagtggatt taaaatcggt tggaatgaac gtagtgatgt
     1441 ctgtagcatt aattctttta atgccgactt tgattggatc aacagaaaaa ctttcaggaa
     1501 taatgtttgc aaagactctt tataatgacg caactagtat caataatcaa gatggcggtg
     1561 tagcatggtc attgattgag cagggagtga cagatttagc ttatattaat aagacagatc
     1621 aatattcgac aattgataaa acaaatgacc gaaataatct aacaaaagaa aatttctgga
     1681 aaacggatct tacgcaagta ttgacagata atgtaattga taaaatggaa aaagagaacc
     1741 caacagctga taatctgcgt tatgaattag ttgaaaattc tgataatgaa tttgttgcta
     1801 ctaaattttc agataatttt ttatcaacct ttgttgatgg tatcaaatca ggatattatc
     1861 gctatcaagc gaacctgtgg ggaatttctt ttggattgat tgctttagcg gtagcgtatg
     1921 tttttagtgc tttcattata attacagcaa tactcgagtt agcatttaaa cgtgtactcg
     1981 gagttttagt atttgctact gacttagaaa caggacaacg atcaaaacaa gttttgtcag
     2041 atattttaca gtgctatttg acagtcggat ttcaagggtt cggtttatct atgtttgcaa
     2101 tgttcataaa ttacctaaat actggcgaag gtatctcagc aaatattttt ataaaaacaa
     2161 ttgcttatat ctgtgctgtg tttgttttaa tcaaaggcag tggaacagtc atgagatact
     2221 ttggtgtgga tattggactc aaagaaggct atgggcaact tgcttctgct tttggaatgg
     2281 gtgcaatgct attccgtaaa ggaacaggtg gtatgaaccg gtctaaaggt gggaaaaata
     2341 atggtggaaa cgatcaagct gaaggggaag aacgaaaacc tgaacgcaac tttggacaat
     2401 cactttctaa aaaagctaat aaaacgggga aagcgcttgg gtatgctcat gaacgtggat
     2461 tatcaggttt agcttcagat ggatctacaa tggctagtga aagagcaaca aaaccattta
     2521 agagtatgcg tgatatagct aatgatacca agagtaagtt taaagaaggt ttagatgatg
     2581 gtactgtgtc cgctattaat aaaaatagca aacctatgtt agctaaaaat aaagaagatg
     2641 aaactggaaa atatgctgat tccatgccaa cacgtctttc ggatagaaaa gatggtgcta
     2701 aggttgaaaa tgccgatagt attatgtcta gttctgaacg tatgcgtgaa gcaatgaaaa
     2761 ataatgctga atcgaataat aatcctgtaa gtgcaattca gcaaaaagtc caacaagata
     2821 ttgaagaacg taaaaatgct atgcacggtc aagctaagtc tgctgaagaa ttaattaatc
     2881 agaagcgtca tgaagcgaaa tacacgcctg aagcaatgaa tcgcgaggaa ataatgttaa
     2941 ggaaacgtgt agaagggcgg acaggcgcta atattgacgg aaaagaagcg ttgaccaaag
     3001 aaagaattca agaagctaaa aattcaaata caggattaga aaaattagtc aaagaaaatc
     3061 cacaaaattc tacaagtagt catggtggcc gatcagtaga tgtccgagaa aatattcaag
     3121 gttcactgaa tggtcatgtt ggacggaatg ttgatgtaag agagaattta caaagttctt
     3181 caaatgctca aggtactaag acaattgatg ttcgagaaaa tctgcaaagt agcagtacaa
     3241 gtcaaccaaa aacagtagat gttcgagaaa atgtacaaaa ttctacaagt agtcaagggt
     3301 ttaaatcagt ggatgtccga gagaatgtgc aaggtagcag tgcaagtcaa ccaaaaacag
     3361 tagatgttcg agaaaatgta caaagttcta caagtagtca aggattcaaa acagtagatg
     3421 ttcgggagaa cgttcaaaaa gatagtggag aaatgaaaga aaaaacacaa aaaatcaata
     3481 tcgttgaaga cagaaagtca gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg
     3541 atagtgaaat ccgtgaaaat gataagggat caaaaccaag acgaaggttt acctatgaag
     3601 acaacgagtt attcagagat acacttaatg atcctaatcc acttttcgat agtttactta
     3661 aaaaataaag aggtgattgt catgcaatta attctacaga ttagtacaca tgatttctta
     3721 gaacttgacg aattatgtga atcagtaaag aaagcaacaa atgaaaaaat cacggtcact
     3781 aattatattg aactgatgat tcaagaagaa ttaaagatga aaaatgaaat agagagcgat
     3841 ttcaaatgtt gctctctaga aaaaaatgtg aggggataat caatggatga taaacaaaaa
     3901 atgtacgaaa aaaatcgcaa acatagtgag gccttaaatt ttcgtgtcga taaaaagact
     3961 tatcttgaac tgacattgat tgcaaaacaa aggggaattt ctagagcaga cgtttgccgt
     4021 gagcggttaa agggactcaa atatgaaccg ccgttgttag ctgaagatga tagggtagtt
     4081 ttaaccaaac agttaaatca aattggtaac aatttgaatc aagcggtaaa ggcattaaat
     4141 atcattgcta aatattttag agaagaatct gaagataaaa aagaaaagag cctacatttt
     4201 agttttgatg ctagccgtta caacattttt aaagatgaac ttgcgaatag catagcaaaa
     4261 aagttgatga ttagttatcc agatgacatt tttttggagt ccaatcggag tgccccctct
     4321 ttttttaaat tatcagaagt agaaaaagaa tatttcgaat attggataga aaagcaaact
     4381 agaccgggta cgaaagaaga gtattcccaa aaagttgatc ggaatttaat tcactcaatt
     4441 cagattattg caagtttaaa agatgaggtg aaacaacttt gggagctact ataacatcga
     4501 aaggacgcaa gaaaaattta tcctcgctat tacaatatgc agctaatcct tcaaaaaaag
     4561 aaaaatgtgt tgcagtctca ggaataaatt gtagtgatga tagtgaggaa gcactgcaag
     4621 aaatgaattt agtacgtacc aagatgaata aatctaaaga tatagcacaa gcgtttcatg
     4681 ttattcattc gtttgataag agtacttcta aagagttaag tattgataaa atgcacgaaa
     4741 tagctgtcga atttgcagag aaagcttttc caaattcgca aatcattgtt gcaagtcaca
     4801 atgataaaga tcattttcat agtcatcttg tcattaataa tattaacatg gaaacatgta
     4861 aaaggttgcg tattgaccct aaagatttag aactaatgcg aaaaattaac gatcaagtcc
     4921 tcaatgaaca tggtcttgaa tcagtgaata atgagtacta tgaaaactta gaaaagagaa
     4981 ccaatcttta ttcaaacgat ccttctaaga tgaaatatgg aaaaattaat tatcaagagt
     5041 ttgtgcaaaa tgcgatcgaa gcaacgttgg aaaaatgcga agtgaattct tttgaagatt
     5101 tttcggatca tttggcaaaa gaatataaca ttgaagttta tcaattttca aaaacaaata
     5161 acaaacttgg ctatgttctt tacaaacaag gtgtaaattt ctatgagcgt agaggtgagt
     5221 taattacttt aggaagagat aaacaatcta gaaaagaaaa aagtcagtat gtcgaacgta
     5281 cttttagtgc aagaaagtta gggaaaagat attcatttga atctgtttct gaacaacttg
     5341 aaaagaattt agaagaatca catataaaaa atttttttaa tatggaaaga gagcaagtta
     5401 tgtcgctaat tagtggagta aattacaaaa ctggtgagtt aaatgtacct tttgtggaat
     5461 tagattccat cgcacaaaag caatttagcg accgtgaagg cgatgaaaaa ttcattaaat
     5521 atgatgactt cttgcgagag gtagacaagc attttgtaaa cacaatgtct ttagaagatg
     5581 atcaagaata cacgattaaa cttcatacgg atgtgaatcc tcaaacttta cgtagtgcga
     5641 aaagtaaaac aatttctatt aaaaagactg atcagaaaga gttatttcta ccggcaaagg
     5701 gttttgaaaa ttgttctcat acaccttctg ctaaacagat tcactatcaa atcagtcgca
     5761 taatgaatga cgatgtaaaa atgtatcgtg ctaaaaagga ttatgaattc acacaacaaa
     5821 tgattgaaaa tcagcaaaaa aggcaacaaa attttgaaat ggaattctga atgggcttgt
     5881 tagtggactt tccagttttt tgcctatgaa gagttgcttg gaaagaagca gtttttagga
     5941 catacgccag ctacccatct acttgccagt caatccgccc cgctcacgct aggctacttt
     6001 ctggcaagtc agatggtggg taaatctctg agggagcaag ctccctacag actccctccc
     6061 aaaaaaagaa aggtggaaga aaaaaatgaa ctatccatat tttaaagtct cggcttcaga
     6121 agaaactaaa gaaatattca acaattttta caaccaaaac aaaggagttt ttggttccaa
     6181 ggcaaatatg tttcgtgtta tggtt
//
LOCUS       DAFCLG010000130         6197 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFCLG010000130 DAFCLG010000000
VERSION     DAFCLG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.132"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..805)
                     /locus_tag="LND56_002407"
     CDS             complement(20..805)
                     /locus_tag="LND56_002407"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK6744280.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(798..1889)
                     /gene="phnW"
                     /locus_tag="LND56_002408"
     CDS             complement(798..1889)
                     /gene="phnW"
                     /locus_tag="LND56_002408"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBK6744281.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            complement(2106..2585)
                     /locus_tag="LND56_002409"
     CDS             complement(2106..2585)
                     /locus_tag="LND56_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBK6744282.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            2944..3726
                     /gene="rpsB"
                     /locus_tag="LND56_002410"
     CDS             2944..3726
                     /gene="rpsB"
                     /locus_tag="LND56_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK6744283.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3825..4706
                     /locus_tag="LND56_002411"
     CDS             3825..4706
                     /locus_tag="LND56_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK6744284.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            4842..5564
                     /locus_tag="LND56_002412"
     CDS             4842..5564
                     /locus_tag="LND56_002412"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK6744285.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            5567..6124
                     /gene="frr"
                     /locus_tag="LND56_002413"
     CDS             5567..6124
                     /gene="frr"
                     /locus_tag="LND56_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK6744286.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         2062 a         1152 c         1257 g         1726 t
ORIGIN      
        1 ctctgatttt gtataaaagt cattctggat tcttgtcagc gtacaaaatg tattctgtta
       61 actcttttaa attgtttaca caaatatcag cgccagcttg ttggaatcta agagcaacag
      121 actgttttaa acgatctttt ttccccatct ctaatttttg gtactcatca taagaaatcc
      181 ccattagcga actaccttca attacaccta ctgtcaacac tccggcattt ttcccttcct
      241 ggatatctga gaccgtatct ccgactttga ttacttcatc aaccgacttg tttttgaagt
      301 actgcatgtt tttatggatc atataaggaa acggtcgacc atacccttcg acgtcttcag
      361 aagttgccat gaagtctggt gaataccctt ctttttttgc ggtttctgct acgattgtca
      421 tcataggagc agtaaatcca gtcgtggaac caatattgat cccagcgttt ctcaaccgtt
      481 cgatcgtttc caatgtgtcg ggtttcaagg tagaatgctt gaccaaatct ttaaacagat
      541 aatgttcaaa ttgttgatac aattcctcca cctctttttc ctgaggtagt tgtccataaa
      601 tagctatcca ttgttttttg acagaaggga gggaaagaag ctgttggata tgatcccatt
      661 ttaattttcc catcggttcg cggatttctt tgtcagtcag ttggattcct ttttctagaa
      721 aagcatttct aaaagcgtgg acaggagcca tacagccaaa gtccacagtt gttcctgccc
      781 aatcaaaaat tacagtttta atcattaacg ttctccttat agtctttgat aattgctgtc
      841 agttccaaaa tatcttccgg atagatctcg ccaatatttc cgatacgaaa acattcatga
      901 tcgctgattt tcccgggata gatcacaaat ccgttttgct ttaaatactg ataaaaatga
      961 ttaaaatcaa atcgccagtc cttagggtaa agaaaagagg tgatgaatgg cccctgatat
     1021 tcttgaatga aaggctggaa ccccatctct gtcatttttt gtcgtagtac atgattattg
     1081 gcgagataac gtgaatatcg agtttcgatt cctccttctg cctctagttc cttcagcgct
     1141 tgttgaaaag ccaaaaccgt atgggtaggg gaagtgaaac gccatttgcc attctcgttt
     1201 tccatcacgc ggtactggtc atatagatca agtgacaaac ttcgactcac attttttcgc
     1261 ttttctagtt ccgctttatt agcaagtaca aaagaaaagc ctggaacacc ttgaatacat
     1321 ttgtttgagc tggaaatcaa aaaatcaatc cctagttcgg ctacacgaat cggtatccca
     1381 ccaaagcttg acatcgcgtc cactataaac aaaagatctt tttcttgtgt gacagcagca
     1441 atctcttcca aattattcaa aataccggaa gtagtctcgc tatggaccat tgctagtgcg
     1501 gagatagagg gattctcttc aatcagctgt gtaatcacat gagcttggag gatttgatgt
     1561 tccgatacct cataaacggt atagttcagt ttcagacatt cgcacatgtt gatcattcgt
     1621 tgtccatatg cgccgtttga acaaatcagt acagtatctt ctggcctgat cacagaactt
     1681 aataccgatt ctaccccaaa ggagccgcta ccttgcatta agaccgttgt gtatattcct
     1741 tccgatactt gagcaagatc caataatttt tttcttatcc attgtgtaat ttctttgtat
     1801 tcttgatccc aagtacaatg atcgatcagc atagcatttt tcactgtttc agaagtcgtc
     1861 aatggtccag gagttaataa tttatacata tgatttcctt tcatttcttt tttgaaatac
     1921 acaaagagca agaagctgct cttgttaaca tccaatataa atgatgatta ttgtgattca
     1981 atcaaggtgt tgaaaaaatt gaataaaaaa tgatgtattt ttgtaaagag tgtaaaggtt
     2041 tatttaataa ggaaaatatt gaaaataaaa aaacacgcat tccatattag gaatacgtgc
     2101 tctgattaaa aaagtgatac ttcagttgga tggtgaagaa attcttctac tcgttttgcg
     2161 tcctcctcat tttccgagat gaccatgatc gtatcgaatc cagcaattgt actgacaata
     2221 gagggaggtt tgatctcatc taaaacagat gcaaataaat gagcattgtc tggcaaggta
     2281 ttgacgatcg tcaaaaagtg gactcgttcg actttcaata caatgtcttc aatcatttga
     2341 accagccgat cctcttcctc agtcctagtc tcaggagttc ctttttcgcg aaacaatacg
     2401 aagcgggaaa cgccattttc atcaggtgcc ttcacgattt ttagatcacg gatatctcga
     2461 gaaatcgttg cctgcgtggc ggtaacgcca taatgcccaa gtttgtttaa tagttcttct
     2521 tgcgtacgga tcgggaattc ttcaatgact tgtttaatca gcaaatgtct ttgagctttg
     2581 cgcattttct ttctccttta cgaaagattt cacatgtatc ataacaaaaa aaagaaggat
     2641 tgcccaataa tattcgtctt ttatacctaa aattatttta tggaaaaagg ataataaaga
     2701 aacaaagaga aatctactgt ttttcttgaa ttctttttat ctttcaggta aactattgaa
     2761 tgttgagcag aaaagtatat ttgctcttgc gatacggatc ttttgtctgt ataatagctg
     2821 agggtgcttt atgcatcagc aaatccacat tcttattgga ttgtcggtag gtgcttgaaa
     2881 aagtttgccg gcaaggtaga agataagaag aggaaaaata aaaccaaatc ggaggaaaca
     2941 aacatggcag taatttctat gaaacaatta ctagaagccg gcgtacactt tggtcaccaa
     3001 actcgtcgct ggaatccaaa aatgaagaaa tatatcttca cagaaagaaa cggaatctac
     3061 atcattgact tgcaaaaaac agttaaatta gtagatgcag cttatgatta catgaagagc
     3121 gttgctgaag aaggcggcgt agcattattc gtaggtacaa aaaaacaagc acaagaagca
     3181 atcaaagaag aagcaactcg tgcaggacaa tacttcgtta accatcgttg gttaggcgga
     3241 acattgacaa actgggatac tatccaaaaa cgtatcagcc gtttgaaaca aatcaatgca
     3301 atggaagaag atggaacttt tgaagttcta cctaaaaaag aagttgctgg tttgaacaaa
     3361 gaacgtgaac gtcttgaaaa attcttaggc ggtatcgctg atatgccaag aatcccagat
     3421 gtaatgtaca tcgttgaccc acgtaaagaa cgtattgctg ttcaagaagc acacaaatta
     3481 aatatcccaa tcgtagctat ggttgataca aactgcgatc cagatgaaat cgacgtagta
     3541 atcccttcaa acgacgatgc aattcgtgcc gttaaattga ttacttcaaa aatggctgat
     3601 gctttcatcg aaggaaacca aggtgaagat caagtagttg aagaagattt cacagaagaa
     3661 aacacagcaa catcaatcga agaaatcgta gatgttgttg aaggcgacaa ttcttcagca
     3721 gaataatttt gaatgatgga gctgtttcaa aggctgggcg acaagcctca ctctcctttg
     3781 aagcagcttt ttttaaaaag aaaaacacac aggaggagtt aaacatggca gacgttacag
     3841 ctaaaatggt aaaagaacta cgcgaaatga ctggcgtagg aatgatggat gcgaaaaaag
     3901 cattagttga agtagaaggc gacatggaaa aagcagttga tcttttacgt gaaaaaggaa
     3961 tggcaaaggc tgcgaagaaa aatgaccgca ttgcagctga aggattggct tcagttgctg
     4021 tgaaaggtaa cactgcagca atcgttgaag taaactcaga aacagacttt gtttctaaaa
     4081 acgaaatgtt ccaagattta gtaaaagaaa ttgcagaact tgttgctgaa aacaaaccag
     4141 cagacatgga agctgcaatg aaaatcaaaa cagataaagg tacaattgaa tctgacttga
     4201 tcgaagcaac tcaagttatc ggagaaaaaa tcagcttccg tcgctttgaa gttgtagaaa
     4261 aagacgacaa tgctgcattt ggtggatact tgcatatggg cggacgtatt gccgtattga
     4321 ctgttctaga tggaacaact gacgaaacag ttgcaagaga cgtagctatg cacgtagcag
     4381 ctatcaatcc tcgttatgta aacgaatcac aaattccaga agctgaatta gaacatgaaa
     4441 aaactgttct tacagaacaa gcattgaacg aaggcaaacc agcgaatatc gttgaaaaaa
     4501 tggtagaagg acgcttgaag aaattcaaag cagaaatcgc tttggtggac caaccattcg
     4561 ttaaagatcc tgatatgact gttgaaaaat acgttgcatc aaaaggcgca actgttaaaa
     4621 cattcgtacg tttcgaagtt ggcgaaggta tcgaaaaacg cgaagataac tttgtagaag
     4681 aagttatgag ccaagtgaaa aaataattct gttgaataac agacgggaac gcacggtttg
     4741 tgcgctccct ttttttaagc aaaacaagaa aaaccagtct tagagtgtcg aaatcatcta
     4801 agtatggtaa aatggcaata gaaaatattg gaggaatgaa catggtcaca ccgaaatatc
     4861 agcgtgtagt cttaaaattg agtggagaag ctttagctgg ggatgcagga tttggaatca
     4921 aaccaccagt aatcaaagaa atcatccaag aaatcaaaga agttcacgag ctgggcgttg
     4981 aaatggcaat cgttgtgggt ggcggaaata tctggcgcgg acaaatcgga gcacaaatgg
     5041 gcatggaacg tgctcaagct gattacatgg gtatgttagc tacagttatg aatgccttag
     5101 ctttacagga tacgttggaa aacgtgggtg taccgacacg cgtgcaaact tcgattgaaa
     5161 tgcgtcaaat cgcagagcca tatattcgcc gtaaagcaga acgtcattta gaaaaaggac
     5221 ggatcgttat ctttgcaggc ggtaccggca acccgtactt ctcgacagac acaacagcag
     5281 cattacgagc ggcagaaatc ggcgcagatg ttatcctgat ggctaaaaac aatgtagacg
     5341 gtgtttattc tgctgatccg aaagtagatg caaatgcagt aaaatttgaa gaactgactc
     5401 atctggaagt tattgccaaa ggattacaag tcatggattc aactgcaagt tcactaagca
     5461 tggataatga tattccactg cttgtcttca atttaaatga acacggaaat attcgccgtg
     5521 caatcttagg cgaaaatatc ggaacaactg taagggggaa ataaaaatgg cagatgcaat
     5581 tatgacagaa gcaaaagaaa aaatgaaaaa agcagcacaa aatcttcaac gtgaacttgg
     5641 gcaaatccgc gcaggccgtg cgaacgctag ccttttggac cgtattactg tgaactacta
     5701 cggtgcgcca acaccattga accaaatggc atcaatccaa attccagaag cacgtgtttt
     5761 aatgatcaca ccttttgaca agagcattct tcaagatgtt gaaaaagcta ttatggcaag
     5821 tgatatcgga atcagtccaa ccaatgatgg aaatgtcatt cgcttagtta tcccgcaact
     5881 gacagaagaa cgcagaaaag aattagctaa agatgtgaaa aaagaagcag aaaatgcaaa
     5941 aatcgcgatt cgtaacatcc gtcgtgatgc gatcgatgaa tataaaaaac aacaaaaaaa
     6001 tggtgatatt accgaagatg atttgcgcgg attagaaaaa gacgtacaaa cattgacaga
     6061 taacagcatt aaagaagtag ataagatcgc tgctgataaa gaaaaagaat tattggaagt
     6121 ttaataaaat cataaaaaca gaggggcgga tcattccgct tcctctgttt ttatttatat
     6181 ttcttttatt taaaaat
//
LOCUS       DAFCLG010000131         6162 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFCLG010000131 DAFCLG010000000
VERSION     DAFCLG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.133"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..532)
                     /locus_tag="LND56_002414"
     CDS             complement(134..532)
                     /locus_tag="LND56_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744287.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            748..1803
                     /locus_tag="LND56_002415"
     CDS             748..1803
                     /locus_tag="LND56_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBK6744288.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            1823..2176
                     /locus_tag="LND56_002416"
     CDS             1823..2176
                     /locus_tag="LND56_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744289.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2358..2654)
                     /locus_tag="LND56_002417"
     CDS             complement(2358..2654)
                     /locus_tag="LND56_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744290.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2658..3476)
                     /locus_tag="LND56_002418"
     CDS             complement(2658..3476)
                     /locus_tag="LND56_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6744291.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3702..3923
                     /locus_tag="LND56_002419"
     CDS             3702..3923
                     /locus_tag="LND56_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBK6744292.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            3925..4191
                     /locus_tag="LND56_002420"
     CDS             3925..4191
                     /locus_tag="LND56_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6744293.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4304..4843
                     /locus_tag="LND56_002421"
     CDS             4304..4843
                     /locus_tag="LND56_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744294.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            5052..5234
                     /locus_tag="LND56_002422"
     CDS             5052..5234
                     /locus_tag="LND56_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6744295.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            5337..5621
                     /locus_tag="LND56_002423"
     CDS             5337..5621
                     /locus_tag="LND56_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744296.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            5637..5873
                     /locus_tag="LND56_002424"
     CDS             5637..5873
                     /locus_tag="LND56_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744297.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            5895..6149
                     /locus_tag="LND56_002425"
     CDS             5895..6149
                     /locus_tag="LND56_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744298.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         2115 a         1061 c         1003 g         1983 t
ORIGIN      
        1 gcgatcattg ctaatacata agcaaagata aaaatatgtt cgatcagctt aaaccctgat
       61 gcgattaagc agaccaagta aaatagaaat tctcttacag ataatggtaa ttttctaact
      121 agcatactca taattaacac cccaaagtaa tattgtattt tgaacatttt acatccaata
      181 cagcatcaat caaccattgc caatcaaaaa tacaaggctt cattgaagta tgtttttcag
      241 atgaaaagat ataatataat ttcttcggaa taatattttc agttgttggg atcaagctaa
      301 atttaatacc atctaactca tagctaccag ctttctctcc attaaaaatt tccttagcac
      361 tttcttctaa agaatcaaaa tatttttcga cagcaacctt tgaagctttt actattgaag
      421 catttccctt ttcgataaaa tctgcttttg ttttaccgac aataagtaat tcttgatcag
      481 taaaatcttc taaaataata gtagtgtaat tcttatttct tctcaatcgc atcaggatca
      541 ctccttaata taatttatgc ttttataata tcataataag ataaatttgc aagtgtttat
      601 ttacaccttt ttatttacac tttattgtaa ttgattatac ttttttgatt gcattttttt
      661 cttataaaca gttgcaacta ttagtaaata ggtgtaaact attttcgtac cgaaatacca
      721 taaacacaaa cagaaagagg tcataccatg aaagaaccat tattgttttc cgttgactta
      781 gggaataaat cagtaaaaga aatgagtaca cacgtttctc cactctcgct tccctctcgt
      841 attttgaaca aaaatatcat taattcagga cgtttttcta atataggttc ctcattaaaa
      901 cgatcactaa acgtttctga atatagccta gtaaacacta ctgaaagcta ttattttggg
      961 aaggatatac acgagctagg aaaagatgac tacgtgatag aatcactagg ttttgggatc
     1021 agcagataca agcaggaaac ctaccaagat atcctatctt tctctttagc taacctagca
     1081 cgtttagaag atcaatctga tgacgtttta accgttaatc tagttttagg tctgccttca
     1141 tccgactata ccgaaacaat cgtcaaatac gtgatgcaac aagccacacg acaacattca
     1201 gttgaagttg acggtaaagc ttataatata aacgtagata ccgtttgggt actgccacag
     1261 ccgattggaa cactatataa cctcctcctt gctgatgatg gtagtgtaca agatgaatca
     1321 ttgatcagtc agaagatcgg ttttgttgat attggtggtg gcacgaaact ttttgatgtt
     1381 gtccaaaatt ttgaaattga cgaaaacaac cgtgaacaaa aggataccgg tatttttact
     1441 ttatataatc gtatcgtaaa tatgcttgac cttccgatca gtcctaatgc tttccaactt
     1501 gaaaagatta tccgagaagg caaagaaaaa ggcgtatatc tatttaaacc aagcggaaat
     1561 gaaagttttg atatttcaga agtagttgaa agagaaataa aaacatacac caatatggtt
     1621 gttgcagaag ttttagctac attcaaaaac ttcacaacct tagataaagt tgtctttact
     1681 ggtggtgggg cttctattat cgacaaacaa cttgtaaaag ataaaattcc gcataccttg
     1741 tttgttgaag acagtgaatt ttctaatgta aaaggctttt acaagtttgg tttaactcaa
     1801 taaaacagcg aaggagatca acatgaataa gggaaaatca gagcgttatg tagttataat
     1861 caatagagaa aaggttagcg agttaccttt agcagagttt cttgaatctg aatatgaaac
     1921 actaggcgtt aaatactctc agacgatcaa aaaagctttg aatctatatc ggcgtgtaaa
     1981 gaacaatgaa gttacaatta ttgaagcagg caacgataga aacgatagca aagtagctga
     2041 agtaaagaat aaaccaactg atcataaccc tattcaacaa gcgcatcctt caacggttac
     2101 atccgatcat tcagcaaaga aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa
     2161 caaagactta ctttaaaaat aaaaaggcga ggttacaata cctagccttt ttattatgta
     2221 caaatacaaa cataaacata tacgtataca tgtatataaa catgtatacg tatatatcca
     2281 taaacacgta tacgaaattt agttaagcta tacctaaccg cttattccaa taaaaaaagt
     2341 gatcagcttt aaactgatca ctcaccaaaa acttcatcat acaagttatc tatcatttca
     2401 ccaagtaaag ctgaatctga tcgaattcct aacttttcgc tgattttaga tagtttcttt
     2461 ctttgtgatg gttgaagcgt gaattgataa tttttcttct tttcagaact atcacttttt
     2521 agtttagcta ccaaagaact tgttgtttct ttaggtgttt gtttaggttc ttcaactgct
     2581 tcaaccactt caggaacttc ttctacttca gcttcagcga attttttaaa tctattttct
     2641 ttcacttcaa tcactcctta ttcttgtgcc gtatcaatta ttttgttgta tagcgtaaac
     2701 gtgctttcaa agaaatcctt attcttacga tagatattct tatccttagc gaactcagca
     2761 atattcttac cttctaagct agttgcatta agcaattcct tctcttgtac atatccaaga
     2821 taattagata acgttggtac caattctagg aactcacgac ttgaattagt attatgtttg
     2881 atcctgctac cgatcgtagc gtatttagtt tccatcagtg tttcaccagt cttaatatca
     2941 atcaactgtt ttgctagtgc atcaatatct ttaccaagct taagcaaagc ttcaaaacca
     3001 tctttagatg gatcagaaat gcctacgaca agatcagata cagcccaagc attttgagct
     3061 agtagacctg catcattatg agtatcaatc agaattagat catacttctc tttaagctgt
     3121 tcatagttaa caacgatcca tctaaacagt agggttctgt tgttaggcat atcacgcatt
     3181 tgcatctcaa cttgttttaa atcttcacct gctactaaaa tatccatgtt ctcatttata
     3241 gctaaaggtt cagctgatcc atgaagatat aaatgattga ttgtattctc tcttttcgct
     3301 ttactgaaag catcaggaaa tcgttttgat aggttttgcg acttatcttc atcaatatac
     3361 agcaccttca tacctaaatt ttcgacagca tatccaccta gattgtagat tgttgttgtt
     3421 ttacctgttc cacctttttc aatgtttcca gtaatgattg acaacttctt ttccatgatt
     3481 aaacctccct tattaatctt ttgtaactcc attatacact ataaacacgt ttatgtatat
     3541 atttatatac ataaacgtgt ttatatttat atttgctttt atattctaca acataaacat
     3601 atatatgtat atgtttatgt ttatgtatat attcaaatat ataactattg catttgtcat
     3661 acaatcgtag tacaatgtaa ttgaaaagga gttgataaac tatgccagta ataagtattc
     3721 gtgtatctaa agaagaaaaa gattttcttg aaaaagttgc tgaattcaac ggagataaca
     3781 tttctgaatt tatgcgtagt aattccctgc aaacagcaga aacgttagta gactttgcca
     3841 cctacaagca actaatgaaa gagcatgagg aaaacgatca aagtatttcc cacgaagata
     3901 tgctaaaaga gttaggctta taagatgact taccaagtaa gatatgaacg tgaagctcaa
     3961 aaagcgctaa aaaaaatgga taagtttcag gcaaaggtta tcctaaactg gatcgagaaa
     4021 aatcttgtag gcactgatga tccaagaaag catggtaaag gtctaacagc taataaatca
     4081 ggctactggc gatatagggt aggtgcatac cgtataatag ctgatattag cgagcatgaa
     4141 gtgacaattc ttatattgtc tattggtcac agaagtgaag tttataagta acagacaagc
     4201 tgataaggct tgtctgtttt tttgttaacc caacaaaatc actgtttacg atcccacttt
     4261 acctaaaaaa gaacaatatc ctcttgatat tatacacacc agtgtgtata atataagtgt
     4321 aaacaagcta attaactttc aaaaggagcg attttttatg ggatggtatg gtgattattc
     4381 atcagcaaat gaagtgatcg aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt
     4441 aggtaccaaa gtaacgaaga cctatggaat tatccttttc aaagatgcag aaggaaccta
     4501 cgatattgac agcttcatct ttcgagatga aatgtacaaa ccaatctact tcttaaatga
     4561 cagtcctgct tactttaaga aaattccgaa aaaatggttg aaattctttg tcggtgatca
     4621 agaaaaaatg gttaagctac aactagatag aattaaagaa gaaagcaata aaccaaaact
     4681 tgatgactta ttagttgcag gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt
     4741 ctatcagtat aaactcaaac gtagctttat ttttcgacta caagatggat cactcactcg
     4801 gttcactcga ctatcagcag gagatgttaa ggaagtaacc taaaacttaa atacataaac
     4861 ataaacgtgt atacgtttat gtttatgtat atgtatatga atataaacgt attaggtacg
     4921 aacactttta aattcttttc tgggctttgt ttttagggta gggctaaaaa aaacatcacc
     4981 atctgatcca aaacagctca gaaacgcatt taaacgtatc tgataccaat attacagaag
     5041 gagtgtatcg tatgattaac ctatttcttt tagtgacagc tagtacatta ttctttactg
     5101 gaatatcact gataatttcc tatttgatct ataagaaagc cgaaaatcac acaagcaaag
     5161 caatcggact agccgtttca tcctttttaa ctttgctgac tttatttgtt actttatcgt
     5221 tttcttttgt ttagaacagc tgatccatta gcaccttttt ctctttacat tatacacact
     5281 gttgtgtata atgtatttgt aaacaagcta attaactttt aggaaggagt gacaagatgc
     5341 taacagctac tttacagcaa cctactcacg ttgctattgc agaaagttat acaattaatg
     5401 cagggatcgt taaacagctg atcaaggatc gagaattcaa aaagtttaac agtcggcaag
     5461 cgtataacag acagctaaag aaagctattc taaagcacaa agattccttc atcgggaact
     5521 tggaaagtgt agatgttaaa actgtttcaa cggcattttc agttgttgaa gtgactattg
     5581 acggtcgctt gatttctcta tttgcttttg gaattaattg atagggagtg tgaactttgg
     5641 aaggtaagac ttttgcagaa atttggactg aatttattga ggtaccaact cgcattgatc
     5701 cgataacttc tgaaaaggta ttggatgcag acttctacaa tctcaaagca ggtactagcc
     5761 ttgaatgcgt ttggatgtat ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct
     5821 tataccacaa tggtgccgag tgggatgcaa ttggaaatat ccatttttct taatataaga
     5881 aaggagaaca caatatgaat tataccgatg aagaacaaat cgctttggaa tacgttttca
     5941 caaatgaaga aggggaggaa attgaaaaag tcttgatcat ttcatcaaaa gacttggaga
     6001 aggctttggc ttatgaacca tacgaatcaa ccgtaactgt cattgaattc ttagatacct
     6061 atacttctaa tgatacttgg gaaattatga atatttgcaa cgctaataac attcaatttg
     6121 ctgaaaaaac caataaatca ttttattagt tttgcaataa aa
//
LOCUS       DAFCLG010000132         6115 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFCLG010000132 DAFCLG010000000
VERSION     DAFCLG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.134"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            375..932
                     /locus_tag="LND56_002426"
                     /pseudo
     CDS             375..932
                     /locus_tag="LND56_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            990..3029
                     /locus_tag="LND56_002427"
     CDS             990..3029
                     /locus_tag="LND56_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBK6744299.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3138..4289)
                     /locus_tag="LND56_002428"
     CDS             complement(3138..4289)
                     /locus_tag="LND56_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6744300.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4443..4973)
                     /locus_tag="LND56_002429"
     CDS             complement(4443..4973)
                     /locus_tag="LND56_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6744301.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5072..5422)
                     /locus_tag="LND56_002430"
     CDS             complement(5072..5422)
                     /locus_tag="LND56_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBK6744302.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5579..>6115)
                     /locus_tag="LND56_002431"
     CDS             complement(5579..>6115)
                     /locus_tag="LND56_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6744303.1"
                     /translation="KGIAYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYV
                     LEFLPELKADQILQDKLVSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAV
                     SGVFILVGLLPNTDWLDRTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVS
                     MGSGATAALGAFDYLMRT"
BASE COUNT         2160 a         1154 c         1148 g         1653 t
ORIGIN      
        1 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
       61 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      121 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      181 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      241 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      301 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      361 gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac
      421 aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa
      481 tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa
      541 aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg
      601 attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt
      661 tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt
      721 atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag
      781 tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag
      841 cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt
      901 gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa
      961 ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc
     1021 gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt
     1081 tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc
     1141 aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa
     1201 aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agataaatac cagaatatat
     1261 acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg
     1321 attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag
     1381 atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac
     1441 ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag
     1501 cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa
     1561 aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca
     1621 aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa
     1681 gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac
     1741 ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag
     1801 ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat aagaatccag
     1861 aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc
     1921 ttcgtgggac aacaggtggg aaattaagca ttacagatgc agacggtgta gagaaaaaag
     1981 ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg
     2041 cacaaaaaat agcttttgac agtctaggag gaaaagctgc atcaactgtt gcgacaacgc
     2101 caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga
     2161 caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca
     2221 tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaatgatt acagcagcaa
     2281 tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat
     2341 ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt gatgtatcac
     2401 aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt
     2461 tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc
     2521 ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag
     2581 atatcttgct agctgatact ggatatggac agggcgaact tctaattact cctatccagc
     2641 aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct aaattgattg
     2701 cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg
     2761 tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt
     2821 tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa
     2881 aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg
     2941 tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca tcggaactat
     3001 tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact
     3061 gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca
     3121 acttcttttt gatttactca cacttcataa actttttttg taaaatattt gagaacaagt
     3181 ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg
     3241 atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc
     3301 ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa
     3361 taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt
     3421 tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata
     3481 aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga
     3541 ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct
     3601 acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac
     3661 actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa
     3721 acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc
     3781 cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca
     3841 gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact
     3901 tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt
     3961 cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca
     4021 agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc
     4081 agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg gactagaata
     4141 tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca
     4201 atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat
     4261 atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa aatatagccc
     4321 gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac
     4381 aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat
     4441 gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg
     4501 aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac
     4561 aataagacga atccgccaat caacaacaga atactactat aaacactcca acttttagct
     4621 tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga
     4681 agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc
     4741 aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg
     4801 taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg
     4861 ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca
     4921 gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa
     4981 aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa
     5041 tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt
     5101 tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat
     5161 cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat
     5221 tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt
     5281 ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta
     5341 tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg
     5401 taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact
     5461 tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa aaggattgtg
     5521 acaaaattct tgtcaccatc cttttttccg ctctctgact ttttacaatc ttcatagctc
     5581 aagtgcgcat cagataatcg aatgctccta aagctgcagt agctcctgaa cccattgaaa
     5641 caatgatttg tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg ccggggatat
     5701 tcgttgcgcc atttttctcg gtcactattt catttcttgg attcatttcg atcgttctat
     5761 ctagccaatc cgtattaggg agtaagccga ctagaatgaa tactcctgaa acagctagtg
     5821 tatgcatctc catcgtacgg cgatcgatat aggagagaga ttctacctga ttcgtaccgc
     5881 tgatttcttt cgtcgctgca tgtttgataa cagtgacatt cgcaagagag actagtttat
     5941 cctgtaagat ttgatctgct tttaattctg gtaagaattc tagaacatat acatgttttg
     6001 ctaacccagc caaatcgatt gctgcttcaa caccagaatt accaccaccg ataacagcaa
     6061 tctcttttcc tttaaataaa gggccgtcac agtgtgggca ataagcaatt ccttt
//
LOCUS       DAFCLG010000133         6079 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFCLG010000133 DAFCLG010000000
VERSION     DAFCLG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.135"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(256..543)
                     /locus_tag="LND56_002432"
     CDS             complement(256..543)
                     /locus_tag="LND56_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6744304.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(563..787)
                     /locus_tag="LND56_002433"
     CDS             complement(563..787)
                     /locus_tag="LND56_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBK6744305.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1242..1412)
                     /locus_tag="LND56_002434"
     CDS             complement(1242..1412)
                     /locus_tag="LND56_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744306.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1427..1960)
                     /locus_tag="LND56_002435"
     CDS             complement(1427..1960)
                     /locus_tag="LND56_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6744307.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1981..2925)
                     /locus_tag="LND56_002436"
     CDS             complement(1981..2925)
                     /locus_tag="LND56_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBK6744308.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3562..3648
                     /locus_tag="LND56_002437"
     CDS             3562..3648
                     /locus_tag="LND56_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6744309.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3769..4458)
                     /locus_tag="LND56_002438"
     CDS             complement(3769..4458)
                     /locus_tag="LND56_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744310.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4465..4743)
                     /locus_tag="LND56_002439"
     CDS             complement(4465..4743)
                     /locus_tag="LND56_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744311.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4703..5617)
                     /locus_tag="LND56_002440"
     CDS             complement(4703..5617)
                     /locus_tag="LND56_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6744312.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5599..5979)
                     /locus_tag="LND56_002441"
     CDS             complement(5599..5979)
                     /locus_tag="LND56_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK6744313.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1610 a         1124 c         1024 g         2321 t
ORIGIN      
        1 cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt
       61 tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt
      121 tttcgttgtt caaagattct tatgccattt actattaata aatctataaa tatgataatt
      181 tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt cccattttca
      241 ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga
      301 ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt
      361 taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc
      421 ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat
      481 agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc
      541 cattataata acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac
      601 cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt
      661 ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac
      721 cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct
      781 ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt
      841 agtaatttta ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt
      901 ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca
      961 taatgtcgat actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat
     1021 ttttattggt ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc
     1081 taattctttg tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt
     1141 gttatgcttt ttacatatct catattcatt tattattctt ttctaaggtt aataatagga
     1201 taatctcttt cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct
     1261 acaaatactt cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt
     1321 ttatttcctt tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg
     1381 atagaaccga atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct
     1441 gccaccattt tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag
     1501 tcgttgaact attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt
     1561 tttcgatttg ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta
     1621 tatgagaatt attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac
     1681 gcaactgatc tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat
     1741 cgttgttttg ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag
     1801 caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca
     1861 tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat
     1921 ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg
     1981 ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc
     2041 ggactctgtt tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag
     2101 gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc
     2161 gtattctttt tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc
     2221 atgtttgtta aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat
     2281 aacaggtttt ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc
     2341 agttagttct tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact
     2401 tttaggaata tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt
     2461 tctgtattgc tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa
     2521 ttgttgtaaa gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc
     2581 gtaaattcgt atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa
     2641 cattacaaac atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa
     2701 gattttttca taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta
     2761 gttacttaaa tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg
     2821 cattctagac acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg
     2881 aattgtattt aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc
     2941 caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa
     3001 aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
     3061 agacaaaatg tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg
     3121 taaagaggtt taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg
     3181 cctaaaaacc acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc
     3241 aaaaccttgc ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg
     3301 cgcagtcgcc taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt
     3361 ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta
     3421 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa
     3481 acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3541 gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt
     3601 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3661 cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct
     3721 aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg
     3781 ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt cttttttcgt
     3841 tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt aggtaactga
     3901 ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat cactgaataa
     3961 attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg
     4021 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     4081 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     4141 ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct cctttttctt
     4201 tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac ttcttgaaat
     4261 acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata atgtaaatat
     4321 aacccctggc aataaaaacc tcttaaatcg aattttagag ccttttaatg aatatcttgt
     4381 ttgataagcc ttatagctaa gccaaataat tcctaaaata ctggcaaaaa tgagtagata
     4441 gggtaaatag aatgccattt ctatttattc tccccatacc agccaaaagg ctaacagtgt
     4501 catgattaag caacataagg gggcaagaat atccattgat cgctgcataa ttactttttt
     4561 ctgttcttta tttcgctggt ttaatagcgc atggtggaat tttttgactt gtgaatcaat
     4621 tgtagtttgt attctttctt tggtataagg tgcaagaaaa gcacgttgtt tctttatttc
     4681 attttctatg tcttgtaacg ttttagaacg ataattcttg ttggtagtct gctcctcttt
     4741 caacgtgttg gtcatggttt cttcgagtcg gtctaatttc cggttgttca gtcgtaagag
     4801 ttcgttgttc tcttcgtact tcttttgcag tttcgcgaat gctagctcga gctcttcgta
     4861 gctcgttggt tcgttgttga ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg
     4921 tctccttttc gtaatccttt cctagtttgt tggatcgtac ttttttcttt ttgtggatca
     4981 attcgtaagt aaaatttttc ccacgattca agcaatgaat gccgaatgtt ttaagagaat
     5041 cacaaaattg gttatacgtt cgagtttttt tgtttttcat tgtttgatca atcgcaaacc
     5101 gaatttcgtc tttccaaaca ggttcatttt tttcttttaa tttgatttca gacatggttc
     5161 gtttttcggg tttcttccct cgttcagtga ttgttaagtc atgttcaaga caaatctcgt
     5221 catttaggtc ataacatttg tttaaaaatg cttgaccatg ggcttgaaat ttgttcccag
     5281 tttccagatt gacggagtta atgacaatgt ggttatgaat atggtctttg tctgtatgtg
     5341 tgtaaatcgc gatttggtgg tcaggggcta tttttttcgc atactctgcc cctagttgat
     5401 tggctttttc agcggtaatt tctcctggtc gaaaagactg gatcaataag tgtgcttgta
     5461 cacgatcgtc ttttccatac agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa
     5521 tgtcacagtt tatccctgtt ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc
     5581 tacaagaagt ggttcggctt actttaactg ttgccatatc ttttgtagct ccttttgcat
     5641 gatttttagg ttttcggata agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt
     5701 gttgcaccac ttggcaattt gattgatatt cccgccagct ttggctaaat cgcgcgcgat
     5761 tgcctgagca atgtctggcg ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc
     5821 tttcacgaaa gccggcacac tcatttgcaa agcttcagct gactgcttca gctttgcaaa
     5881 ttcggattcg ctcactcgaa aactgatttg ttttggctcg gatcgatagg tatatttttt
     5941 agaatgattg ctagccaaat attgattttg ttcgctcata agtcccaccc tttcagtacg
     6001 tcctacggac gtatcgttcg caataccatt gtacctaaaa aggtacaata agtaaagctc
     6061 actaaagctc actaaaggg
//
LOCUS       DAFCLG010000134         5980 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFCLG010000134 DAFCLG010000000
VERSION     DAFCLG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.136"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(311..2002)
                     /locus_tag="LND56_002442"
     CDS             complement(311..2002)
                     /locus_tag="LND56_002442"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK6744314.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(2424..3371)
                     /gene="arcC"
                     /locus_tag="LND56_002443"
     CDS             complement(2424..3371)
                     /gene="arcC"
                     /locus_tag="LND56_002443"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK6744315.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(3486..4505)
                     /gene="argF"
                     /locus_tag="LND56_002444"
     CDS             complement(3486..4505)
                     /gene="argF"
                     /locus_tag="LND56_002444"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK6744316.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(4596..5825)
                     /gene="arcA"
                     /locus_tag="LND56_002445"
     CDS             complement(4596..5825)
                     /gene="arcA"
                     /locus_tag="LND56_002445"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBK6744317.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         1617 a         1276 c         1035 g         2052 t
ORIGIN      
        1 actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt tcgtttggtt
       61 aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaagggagct
      121 gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta agagacaacc
      181 tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat
      241 ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat
      301 tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca
      361 ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta
      421 aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg
      481 caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa
      541 ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag
      601 ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag
      661 gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc
      721 gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat
      781 ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt
      841 catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac
      901 cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca aagcccattt
      961 ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata
     1021 gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa
     1081 tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata
     1141 ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga
     1201 cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt
     1261 ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata
     1321 attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat
     1381 ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga agcaattcat
     1441 tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt
     1501 ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt
     1561 tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga
     1621 ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatat
     1681 tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa
     1741 gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg
     1801 cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt
     1861 ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta
     1921 gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg
     1981 cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga ttgttaaaca
     2041 aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg
     2101 gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc
     2161 tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca
     2221 cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct
     2281 tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa
     2341 aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc
     2401 gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct
     2461 aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca
     2521 acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa ttgcttttca
     2581 tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt
     2641 tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg
     2701 atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat
     2761 ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct
     2821 tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg
     2881 actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc
     2941 atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg
     3001 aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg
     3061 atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc gatagatcct
     3121 tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct
     3181 tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca
     3241 tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg
     3301 gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac aactactttt
     3361 cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg
     3421 agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt
     3481 tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt
     3541 gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca
     3601 tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg
     3661 tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt
     3721 ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat
     3781 ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca
     3841 cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct
     3901 gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc
     3961 gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg
     4021 cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca
     4081 gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag
     4141 aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat tccatcaaac
     4201 atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca
     4261 tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa
     4321 cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga
     4381 tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt
     4441 aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct
     4501 ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac
     4561 ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc acggacaagt
     4621 ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga attgatttct
     4681 aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg
     4741 actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt
     4801 gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca
     4861 gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt
     4921 tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat
     4981 ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgtttgga
     5041 attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat
     5101 ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt
     5161 aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg
     5221 attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca
     5281 cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa
     5341 gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct
     5401 gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg aaccccagcc
     5461 atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct
     5521 cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg
     5581 acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct
     5641 tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga
     5701 atatcatcga acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca
     5761 ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta
     5821 tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat
     5881 cactttcata attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt
     5941 aaataattaa acgtttttct atttttaagc gtaatttatt
//
LOCUS       DAFCLG010000135         5967 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFCLG010000135 DAFCLG010000000
VERSION     DAFCLG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.137"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..450)
                     /locus_tag="LND56_002446"
     CDS             complement(<1..450)
                     /locus_tag="LND56_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6744318.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="LND56_002447"
     CDS             674..1465
                     /locus_tag="LND56_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6744319.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="LND56_002448"
     CDS             1431..2384
                     /locus_tag="LND56_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBK6744320.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="LND56_002449"
     CDS             complement(2782..3756)
                     /locus_tag="LND56_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6744321.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="LND56_002450"
     CDS             3871..4305
                     /locus_tag="LND56_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6744322.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="LND56_002451"
     CDS             complement(4430..5452)
                     /locus_tag="LND56_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBK6744323.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5967
                     /locus_tag="LND56_002452"
     CDS             5844..>5967
                     /locus_tag="LND56_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBK6744324.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETH"
BASE COUNT         1875 a         1125 c         1028 g         1939 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatg
//
LOCUS       DAFCLG010000136         5785 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFCLG010000136 DAFCLG010000000
VERSION     DAFCLG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.138"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(192..662)
                     /locus_tag="LND56_002453"
     CDS             complement(192..662)
                     /locus_tag="LND56_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBK6744325.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="LND56_002454"
     CDS             992..2632
                     /locus_tag="LND56_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBK6744326.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="LND56_002455"
     CDS             2786..4108
                     /locus_tag="LND56_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK6744327.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="LND56_002456"
     CDS             complement(4176..4610)
                     /locus_tag="LND56_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6744328.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5511)
                     /locus_tag="LND56_002457"
     CDS             complement(4675..5511)
                     /locus_tag="LND56_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK6744329.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1730 a         1152 c         1062 g         1841 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     5101 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5161 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5221 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5281 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5341 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5401 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5461 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5521 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5581 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5641 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5701 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5761 agaaagaggt cgtgagcctg ccgtt
//
LOCUS       DAFCLG010000137         5724 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFCLG010000137 DAFCLG010000000
VERSION     DAFCLG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.139"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            196..921
                     /locus_tag="LND56_002458"
     CDS             196..921
                     /locus_tag="LND56_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744330.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="LND56_002459"
     CDS             911..1420
                     /locus_tag="LND56_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744331.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="LND56_002460"
     CDS             complement(1490..2938)
                     /locus_tag="LND56_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6744332.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="LND56_002461"
     CDS             complement(2938..3654)
                     /locus_tag="LND56_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6744333.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="LND56_002462"
     CDS             complement(3635..3985)
                     /locus_tag="LND56_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK6744334.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="LND56_002463"
     CDS             4129..4902
                     /locus_tag="LND56_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6744335.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="LND56_002464"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="LND56_002464"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1661 a         1124 c          994 g         1945 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttt
//
LOCUS       DAFCLG010000138         5569 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFCLG010000138 DAFCLG010000000
VERSION     DAFCLG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.140"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(205..660)
                     /locus_tag="LND56_002465"
     CDS             complement(205..660)
                     /locus_tag="LND56_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744336.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1280..1696)
                     /locus_tag="LND56_002466"
     CDS             complement(1280..1696)
                     /locus_tag="LND56_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744337.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1698..1979)
                     /locus_tag="LND56_002467"
     CDS             complement(1698..1979)
                     /locus_tag="LND56_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744338.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2355..2879)
                     /locus_tag="LND56_002468"
     CDS             complement(2355..2879)
                     /locus_tag="LND56_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6744339.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            complement(2896..3864)
                     /locus_tag="LND56_002469"
     CDS             complement(2896..3864)
                     /locus_tag="LND56_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6744340.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(5031..5186)
                     /locus_tag="LND56_002470"
     CDS             complement(5031..5186)
                     /locus_tag="LND56_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744341.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(5205..>5569)
                     /locus_tag="LND56_002471"
     CDS             complement(5205..>5569)
                     /locus_tag="LND56_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6744342.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1675 a          907 c          942 g         2045 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgattttta attggtctag ctagttacta gcttctaatg cttgatttta cgcgatttct
      901 cttttttagt tctttgtgca tttttatctg tcatttttta aagacttgtt atttttgaga
      961 ggatttgata tgcttttttt gtctttctct agttcttcac tttgtaatat ttttatgttg
     1021 atgaatagga taatctcatt ttgggctgct cgtgagagca gtcttttttt gtttcagggt
     1081 acgtcctatt ctttggattt ccgcgctatt aaataaccga tacttgggga ttggggtagc
     1141 gccccaaaat acaaaaagaa gaagttgcat aagcggattt ttcttttttt tcgcaagttg
     1201 gtaccaactt gcaaatcttg ctggaagact gatccctaaa aagaaatcga ggaatttttt
     1261 tagggatcgg tttctttttt tatcttttta gaaagtttgt tgggtcaaaa tttccagaag
     1321 taaaggtttg attggtttct ttttgacgat ttttgggttg atttcgtcgg gaaggattga
     1381 gttgattttc aattttttct ttcacttctt gatacacttt agaatttgta ccgataagaa
     1441 caccagtatc ttttagatat aaatcagcaa attctgccat gatttctgtc atgatagaaa
     1501 tttcttttcc catggcattg agttttgctt ggttactggt tgtggttgta ttggtatcag
     1561 gttcataact catacataaa aaacgaatgg cttctgaata attgttgacg tctggatttt
     1621 tttcgatgat ggaatctaaa acgtcaacgt gactgggata aagggttacg tgtttttgaa
     1681 cagaagtttt tctcatgtta tattcctccg ataagtgttt caatgagttg gttgtttgtg
     1741 tgaacatagt cattcagtat gttcgtattt tctgtttgaa tggcaataaa tttttcgagt
     1801 aataaatcga attgatcttt tttgtaattc ataagttgac ggtcaaaatc actttcgatt
     1861 agaactttta gatatttatt tcgactccat tttttgcccg ttttggttgt gagttcctga
     1921 caacgtttat ctattcttgc aataattttg ggattgatat ttcgaactaa aatgtccatg
     1981 gaaattttcc tttcgtatca gcgtataatt tatgtatacc atacaaatat agtatttgta
     2041 tagctatttt tcgccaaaac actatacata gcctagccac aatggcaaaa aagtgatagc
     2101 aaattttttt ctgagaaacg tgataggcac agcctatcta gcacctttcg taagaaagtg
     2161 ataccacgtt tccttatgct ctttcaaaat tcagaaaaat cagctgtaaa acgagtgcag
     2221 tccgagtaag attcaacgaa aaaacgggga ttggggcgcc accaaaatat cgcaacatgg
     2281 gtccatgttg ctctgctcgc aaaagcaaaa agaagaataa aaccagcaat ttttattaat
     2341 ttaaattgct ggttttattg ccagaattgc caccattttt tctccgcctt tgattgtttt
     2401 tctatcgatt ctgtttcttc agaaagagtc gttgatttat tttccgaaga ttcttccgta
     2461 taagtcaact gtagttgttc ctgcagtttc tcgatttgtt ggttggcttg cagggtgagt
     2521 tgttgttggt ggtcgagtaa tttttgcatt tgttttattt gctcgtcttt ttcttgtagt
     2581 tgcatatctt tttgatttaa ctgttgttct aggagagaaa ggacttctaa ttcgggaact
     2641 tggttggcgt ttttgtggtt ttcggttttc gttttttcgc caactagttg ttgatttgag
     2701 ttttcgtttt cgttaaaaaa cattgatttt atagcctttt gtccggtttc attaatcaca
     2761 aacttgtttc cgattttcga aaaccaaagt tttcgatttt cgtcagaaac ttttttgtta
     2821 attgctgttt tactaactcc taattcttca gcaagttctt tgattgtttt ttctgtcata
     2881 aaatctaaaa acccctcaat ctttgaataa gttttgaaga ttgtctaata agtctttttt
     2941 tagttctgaa ggaatatcgg aatttatctc tgatttttgt gcattatttt ctttattttg
     3001 ggtattttgt tctctttcta acttttctaa ctgttgtaaa agttccaatt ttcgcttttg
     3061 ttctttgatt ttttcttgta atgaagtagg tgctttttca gattgaactt gttggttagc
     3121 tagttctaat gctttgtttt gttcttcttt tgttggggca tgtttgttaa agtcgtctga
     3181 tttttttatc tcaggtttaa atgtaaattg atagccaatg acaggctttc cccgtccttt
     3241 tccgtatttc tttttgattg ttaaaccttt aaagattgcg gttagttctt gtcgtattgg
     3301 attgaacaca cgttgatcaa tcgtaccttg gttgtaactt ttaggaatat caagtaattc
     3361 atgaaagtct tcttttgaaa aataagcgaa acctttggtt ctgtattgtt ttagtaatcg
     3421 aaacattgtt tttgaataac tgctttctaa ttcattgaat tgttggagag aatagcgcac
     3481 ccattcttca agattattaa gtaagggtaa ggctttttca tgaacttgta tttcagcaaa
     3541 aggaatatca gattttccgt cgattttaaa ttgattaaat aaaacaaaac gttcacgttg
     3601 caaaccgtca gcacttcttc taccaaaacg taaatccatt aatttgtcat acgttgtttc
     3661 taaatcatct atgaatcgaa catttgcagt tgctttataa ttgcttaaat cttttaattg
     3721 ctcgaatgta aattgtactt ttttatcacc tacatcacgc attcttgata cgatagaaaa
     3781 gaacaaattc atttcgactg gtgtaaattt tcgcaacgga attgtattta attcatgatg
     3841 atactttacg atttcattag acatatttaa aatagctcct ttcttactcg cacaagagat
     3901 acacgttatg aaatacaaaa tgcctgttta tctacatctt atattaacat cgtcgatgta
     3961 ctaattcaat gtacaaacag acaaaacgat gtagataaac agacaaaacg atgtagataa
     4021 acagacaaaa cgatgtagat aaacagacaa aacgatgtag ataaaagcgt cattcccttg
     4081 ggagagtaag gatatagccg acggtaaaga ggtttaaaaa gaggtttaaa aagagattat
     4141 aaaagagggg gacaaaattt cccgcctaaa aaaccacttc ttttttctcc aaacagattc
     4201 gcgcattttt ctcaactggg aatcttactc cctacagtcg cttaaaaagg tgtgatcgct
     4261 ctgctctcac attatactgc gcagtcgctt ccggctcctt tgctaaaaat gttcataagg
     4321 aactctttga gaagctatga gccttcgttt gagacgtttt aagacagtct tagtataaat
     4381 cccttatctt gttcttaaag cgccttacag gcggtcaggg caagccctaa caaccctaaa
     4441 gttgtctgaa tgccctctaa aattcgattt aagcactctt aaacctactc ttatatattt
     4501 atatacttag acgtttcaaa cgtcttaaaa cgcaaattag agcttgataa agatatttgc
     4561 tcgtgtgccg acttttttaa aagtctaggc acacgccatg acaaagctga aataaactgc
     4621 ttcagctttg tcatggcgga ttcgctcact tgaaaaagtg ttcatttcgc tcgattatta
     4681 gcatatattt tttaactttt tttgctagcc aaattttgca ttagttcgct cataaatccc
     4741 accctttcag tacgtcctac ggacgtatcg ttcgtaatgt gaaagtacct ttaaaggtac
     4801 tttctacaaa actcactaaa gtgtttggca agctctatat ttgctagcca aatacctacg
     4861 tatttggtgc aaataaccgc ttgtcaaagg ggcgcaagct cccccttgaa tcccccctca
     4921 aaacccacgc tccacaatac aaaaaaggct agcaaatgct agccactttt tgtaaagttt
     4981 cgcttgtttc accttcggtg aaattgttgc tttgcaacga tcaggtagag ctacattttt
     5041 tttcgttgtt caaatattct tacgccattt actattaaaa tcgtaggtat tccaaagtaa
     5101 agaaaaaatt gattagcagg taatacaaaa tataaaataa taccagcaat aattaccaaa
     5161 cttatagtaa gttttgttaa tttcatgaag tatgccacct tatcttatat agatatcaaa
     5221 tagattaata tgccgattgt attataaaat ccatgagcaa ctatggaata ccatatattt
     5281 tttgtttgta tataaacaaa acttaaaata ataccaataa agaaaatact taggcctccc
     5341 ccatgaacaa tagaaaacag aaaagaagaa attagtattg aaatatattg ttgttttttg
     5401 tttgcttgag gatcaatcat acaaaatcga aaaattcctt cttctaaaaa agctccataa
     5461 gtagcagcat ttaaaaagaa taaaaggaga gaaccattta cttcattagg atttatacct
     5521 tctgaaaaag atgtatttag aatattgatt aggaataaaa cagagaatg
//
LOCUS       DAFCLG010000139         5545 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFCLG010000139 DAFCLG010000000
VERSION     DAFCLG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.141"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(448..1257)
                     /locus_tag="LND56_002472"
     CDS             complement(448..1257)
                     /locus_tag="LND56_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6744343.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1257..2003)
                     /locus_tag="LND56_002473"
     CDS             complement(1257..2003)
                     /locus_tag="LND56_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6744344.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2021..2488)
                     /locus_tag="LND56_002474"
     CDS             complement(2021..2488)
                     /locus_tag="LND56_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6744345.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2488..2898)
                     /locus_tag="LND56_002475"
     CDS             complement(2488..2898)
                     /locus_tag="LND56_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6744346.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2909..3922)
                     /locus_tag="LND56_002476"
     CDS             complement(2909..3922)
                     /locus_tag="LND56_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6744347.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4139..4873
                     /locus_tag="LND56_002477"
     CDS             4139..4873
                     /locus_tag="LND56_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6744348.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4857..>5545
                     /locus_tag="LND56_002478"
     CDS             4857..>5545
                     /locus_tag="LND56_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6744349.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1774 a         1171 c          914 g         1686 t
ORIGIN      
        1 ctcggtgatc ttcgcggttt aacaccatca gaagaaatgc acaaaaataa taaggagagt
       61 ctgagacata aataaattcc cagactttta gtgagtctaa atccgaataa acggtgggtc
      121 gaaagcaact ctagctgcgc gtcgatcgca gcaatctcta ggtgctatgt tcggataaat
      181 ggtggtcaaa agtcagattt tcggccattt cgtaacagcc aaccagacaa aaagcgtctg
      241 ttttgtcttc tactccaatt gctcaaatct tagcgacttt tggcttggtc ttactctaaa
      301 cagtttgtaa gcctaaactt tatttgctgt ttgtttctaa gatcaaagca aaactacttt
      361 ttagttggtt acttttacaa gttcttttta ctgttgaata aatgtaaatc ttaagaacaa
      421 atatttactt ctataattcc aagtttctta aaaaattccg ccaagtgtac ctaaaaagca
      481 taaaacaaaa attcctagaa taattgtgat cgttttatgc cctttggagt aaagccagac
      541 gcataaaaag gtaattccta atggcagcaa gcccggaaaa atttgatcaa aaatactttg
      601 taatgaagtt tcagctccac caaccgtgaa ttttagtgac gtatttaaag taaccatcgt
      661 gcctatcatc gcgccaatta ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc
      721 aatcatatgg ttgtcttctg tatcatcaat aaatttagtt cctaaaacat agccatattt
      781 tgtcgccaac catttaaaaa taaaatgaat ggcagtatac aaagtaaaat aaaggatcat
      841 gcctaaaata ttcccttgtt gagcaaaaga aatacctatg ccagccgcaa taacccgaaa
      901 agttccccag aaaaatgaat cgccaatacc tgataaaggt cccattaatc ctgttttgat
      961 tttattaatt gcgtctgtat caaattcagg atttttagca ttttcttctt ccatggtgac
     1021 ggacaagctc ataacaaaag gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg
     1081 ctgcaacgct gtataataat catcatcgtt cggatatatt ttacgtaagg aacgttcaat
     1141 accattcatt tgtccatatg ccatcatttt atcgaaggac caagaggatt gcataaaaaa
     1201 ggagcgccaa aatatacttc ttaattcttt tttcccaata acactttctt ttaccattat
     1261 aaatcttcct cctcttcaaa agttagcttt tgaacagcca gtaacgctaa gcaaagtccg
     1321 acgacagcca caccgacaac atccattttc agatacatta cgccgacaaa tcctaagaaa
     1381 agataaggca tcatcgcttt attcgccatc attttaatta ataacgctag cccaacacaa
     1441 gaaagcacac cggccgcaac atcaaagctg tccaatacaa cctgaggaat caacttgatc
     1501 atcgcattga ttgtagacac accaaaataa gtagctacaa aaactaaaat ggccgatgga
     1561 accccaacga ttaaaggttg agccacataa tgatattttt gtgctttttt gaaatcatga
     1621 ttgactaatg cctcatccat tttgttgtta aaatacgtaa ttggcattgc ataggcgaag
     1681 ttacgccaca tctgaagaaa gatggacaaa ggtaaagtca acgcaacggc agttcctact
     1741 ccttgacccg attgaatcgc aattgctgta cctaaaacac tggaagcata aacttcaggg
     1801 ggcgtcgcac tccctaccat aattgagccc atgaacatgg attccaaggt ggcaccaata
     1861 ataattcctg actctaaatc tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt
     1921 ctacccatca tcgatgcacc aaatgtgtat tcatcaatag acgccaaaaa agctaccagt
     1981 gcgattaata ttgcttgtat catattcttc ccctcctctt ttagtcatgc aaaggattga
     2041 tttttttgtc cgttggtaca gcctgacatt gaactgttat cccgttttca gtcatttttt
     2101 tcaccaagtc cagttcttgt tcattcaagt aaacggaact agtaatcatt ttccgattct
     2161 cgtttttctt taaatttcct aaattcactt ctttaatctc aggtacgtgc tgagtaattt
     2221 cgtaggcatc ctccaccgtc ccgacaatta aaaatacttg gtatttcttg attttttctc
     2281 catttaaaac agagatggcc tctgtcatat ccttcaccac aaattttgtc cctgtaggaa
     2341 cagccatttt tagtgttttt ttgcgcagat tattggtagc gatttcatta cttgcaacga
     2401 aaatacaatt tgcacctaag ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac
     2461 gttcatctac tcgtaataat ttaatcatta aaaatcatcc tcttctgttt tctctagcaa
     2521 ctcgttcacc aaaactatcc cttcattccc gctattcata gctttttgga ttagtttttt
     2581 cgtgtcctgt tcacctgata agcaaagctc taaaagcagc ggcagattta caccgctgat
     2641 taaataaaaa ttgtctttac cgatttgttc ggagaaagcg ttagacacgc ttcctcccag
     2701 taaatctacc gtcacaatta attcgtcatc aggcgctaag tcggaaaata gctgctctat
     2761 ttcctggtta atggagtagt cactatctat atacgcagtt attgctaaaa gtgatttttg
     2821 aggaccaata ataaattcta aagtatcctt taagccttta gccatcatgg aatgcgtcgc
     2881 aattacaatt ttcctcatag ctcttacctt aatattccac agtccgataa taacgacgaa
     2941 tatctagtga atgatcccgc accttttcaa aatgagcact cactcgttct aaggccgttg
     3001 ccataaccat tggcgctaca tactgacgca tagcgcttgg tacacctgct aattcgaaat
     3061 ccgcagtgtc aaaaaccgtc acaacatcgc tatatttatt tacaaaacgc tctacgcgat
     3121 cgactagcgg acgcgtttga tcttcacctt ttaataacac aaaactcata gatttatcag
     3181 tcatttcaat agtcccatgg aaaaattcgg ctgcatggat cgacttggtg gcaatccatt
     3241 gcatttcttc cagtacgcac atagcatatt cataggcagc cccataagta ttcccggctc
     3301 caacaaacat atgaaattcc gtatttttat gtttttctgc aaattcaagt gctttctgat
     3361 cattttgttg ccgaacactt agtaatactt ccggcatttt ttgtaatgtt tgaataaatt
     3421 catcatattc aggaaactca ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact
     3481 gtaactgaag ttcttctact aatgtatcgt tgtcagcatc attttcaatc gcataatcca
     3541 cgatttcccc gagtggtgta gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac
     3601 tattggcata cttaacaaca tcaacggttt cttgagtcgt accggttaat gaagatgtga
     3661 ttaacactgt atctttatta atctttttcg gctgtgaaca aaccacctct gcggctattt
     3721 catgataata atcaagctct gaatgagtgc ttaacagata agctagcggg ctgattgtgg
     3781 catatgttcc accagagcct accaaataaa gatttttaaa tcccttctca ctgattgcat
     3841 ctgccacctg attgattgtc tcgatatttt ttactgcacc atttaatact gatagaaaac
     3901 gttcttcgct aaaacctaac atagttaaaa acctccaagt caataatttg aaaaatatat
     3961 ttgcaaatgc cggcacatta acaaaccact taaccttttt tatataccac ctgttaacgt
     4021 ttttaatgta ccacttatag aaaacgctgt caatagtttt taaccacttt tatttttatt
     4081 tgtaccaaaa tccttttttc atgtataata aacaaaaata gactctggag tgtaacgtat
     4141 gaatactgaa gaactaacga acaaattaat acaagacatt gaacaagaaa aatataaata
     4201 cgcgcaagct attccttcag aacgtgattt tgttcaaatt tatgaaatga atcgttcagc
     4261 cgtaaaaaaa gccattcaac aattagtaaa taaagagtac ctactaaaaa tacatggcaa
     4321 aggaactttt gttaaaaaaa atatccatga acatttgcaa atcaaatttc atggtttgtc
     4381 tgaattgttg gaacgggaag ggattcagcc tagcagctta attttagctt cagaaataaa
     4441 accggctgga cacattgtcg ggaaagcttt acagctggaa ccaactgaag aagtatttca
     4501 tttggttcgt ttaagaagcg gcgacggtga gccaatttct attgaagaca cttatattcc
     4561 attaaatctc atacctgatc ttcaagcttt tgatttccaa attttttcat tttattccac
     4621 attggctgct tatcattatc aaattgaaaa tatctatcag acaatttcca gctcaaaagt
     4681 gagcaataaa gaagcccgtt tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc
     4741 tatcactgcg gttacgacaa atgaacaagc ggttgaatac actgaagtag tggttttgcc
     4801 tgaattcgcc gccatctata ctaaaaatgt tcatcaagga aaggagatga aaatctatgc
     4861 ccaaattgat taatctgttg gcttatgacc tgattgacaa aattattgct tatattcatg
     4921 aacatcattt aacccccgga acaaaactgc cttcggaacg ggacctatca gaaatgttta
     4981 atgttaatcg aactacctta agaggcgcgt tacaaaaact ggtggacaac ggcaccttac
     5041 gggcgcaaac caatagcggc tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc
     5101 atttctttac accgatagaa gattctctgc ttagtaagca gcggctacag ctaaaggcaa
     5161 cggctctttc accgaaaaaa gcagcagctc tttgtccgga actgaatgat ttcaaacagc
     5221 agcaaccaca agcttattta gaatatattg atgcagaagc aattagcttg atgttattaa
     5281 tccctatgga aacattaaag aaaagtaatt tctcggacaa tcctttccaa gattttaaaa
     5341 gtttggcata tacgcgttct caagaaattt ctttaataac tgacaatgac gttgcggcgg
     5401 acttgctggg agttgacaaa gaagaggttc tgttatttgt acgagaggaa ataagagccg
     5461 gtaaggagtt ggcggcgatt gctttaggtt atactattag tcgccgctgc aacatacagg
     5521 ttaatgttgc tacgaccttg caatc
//
LOCUS       DAFCLG010000140         5529 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFCLG010000140 DAFCLG010000000
VERSION     DAFCLG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.142"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..134
                     /locus_tag="LND56_002479"
     tRNA            50..134
                     /locus_tag="LND56_002479"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84..86,aa:Leu,seq:caa)
     gene            389..1264
                     /locus_tag="LND56_002480"
     CDS             389..1264
                     /locus_tag="LND56_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6744350.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1368..2132
                     /locus_tag="LND56_002481"
     CDS             1368..2132
                     /locus_tag="LND56_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBK6744351.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2119..3561
                     /locus_tag="LND56_002482"
     CDS             2119..3561
                     /locus_tag="LND56_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6744352.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3572..4903
                     /locus_tag="LND56_002483"
     CDS             3572..4903
                     /locus_tag="LND56_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBK6744353.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            4916..5374
                     /locus_tag="LND56_002484"
     CDS             4916..5374
                     /locus_tag="LND56_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6744354.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1853 a          816 c         1159 g         1701 t
ORIGIN      
        1 ctcatgcgtt ggttcgaatc cagctacccc agttattatt tttaaatttg ccggcgtggc
       61 ggaattggca gacgcgctgg actcaaaatc cagtgtccgt tgaggacgtg ccggttcgac
      121 cccggccgcc ggtataatta tcaatatagc aactttctta tagagagttg ctatattttt
      181 tttgttttta tgtaattacc tccaaatcaa ttcgatacaa gtattatcat aaaacgagta
      241 aaaaagatat tgataacgct tttattatgt gttaaactat ttttgtaaat tatgtagggt
      301 gctactattc aattaggcac gacctaagaa agggttcttt cttaggtcgt tttttgtctt
      361 taaaaattgt ttggagacaa aaatatgtat gaaggagtct gcaagttgtt ttgttttatc
      421 atatagagga ggtgaattaa cggtgtatgt gatgaaaaag ctgaatcata atgctgtatt
      481 gatcgatgac gatggaatag aaaaaattgc gatggggaaa gggattggct tttctgctaa
      541 agtgagtaag ccatttgatg ttgaggatgc tgacaaatta tttgtattag attctaaaga
      601 aaagatcaaa cgattcagtg agatagtctc gcagattcca atcgaattta tggattttgc
      661 ggaagaagtg attgaactga taacagctag gatctcctct ccattagatt caaatatcta
      721 tattacattg acagatcata tttattttgc tattcaacga aaaagagaag atagagatgt
      781 cacagcaatt atgttgccag aaatgagatt cctttatcca aatgaatatg atatagcagt
      841 agaagtggtc gagcgaatca atcatcgata tcaaaccgag ttagggtcaa atgaagtagg
      901 attcatcact atgcatatca ttaatgctga gctaggagag cctaatagtt taaagagctt
      961 aaaaatagta gaaatgacga ccacgatttt gtcccatcta gaaaaatcct attcaaacgt
     1021 ttatgataaa gaatcactga cttatcatcg cttaatggtt catatcaaat tcctggttaa
     1081 acgcttgatt tatcaggaag aaacgccagt agaaaatatt ggttttttta ctgaaacatt
     1141 taaacatagc aaagcgtatt gtacagcact aaagattaaa gatatcatcg aacagcaatt
     1201 tcaacttgcc attcaagaaa atgaaatcat ctatttggca attcatctag caagaatcta
     1261 ttaaaataag ggcgtaacta tctataggcg caaccaaatg acacatagta aatggactat
     1321 gattatttgg ttgcgcctat tttagtagaa aaaagaagga gaatgaaatg ggaaaagtga
     1381 tttttaattc ggatgatttt ggctatagcc atggtgtgaa ttatgggatt atggatgctt
     1441 atcagcgagg aattttgaca tcaactacat tgatggcaaa tatgccgggt ttcgagcatg
     1501 ctgtgaaact aagaaaggaa atgcctaggc taggtgtagg agtccattta acattgacgt
     1561 gtggcaaacc actcctcaaa actgtcgata ctcttatcga aagcggagag ttcagatcct
     1621 tatcttatta tcagcatcca tttaacattg acaaagatca actgtatcag gaatggaatg
     1681 ctcagattca aaaaatttat catgcgggga tcataccaac tcatttagac agtcatcatc
     1741 atactcatac tttggatgaa aatcaggagg tagttgttac attagcaaaa aaatatgatt
     1801 tgccggttcg gagcaacttt gaacgaaaag atgaagttcg tcatgtcgat tattttgagc
     1861 cttgtttcga tgtcgtagga gaaactgatg aaaaaaaacg acaaatcaat acgagtgtgg
     1921 aagaatattt ggcaagttta ctcatgatat tgagagaaga taaaacgatc gaagtcatgt
     1981 gtcacgcagc gtatttggat caggaattgt taaatggttc aagttttgtg tatcctcgta
     2041 cccaccaagt cgaattttta attcattcag catttgctca aaaagtgaaa aatgatcctt
     2101 tgattgagtt agtaacatat gaggatattt aatagaggag gaataaaaat gaatgttaaa
     2161 tcaaatgtgc agaagcttgg aagagcgatg ctacttccag ttgcagcgat gccattagcg
     2221 ggattgatca tgcgtttatc agctgacgat atgctgaaca tcccagtaat tggtgcagct
     2281 ggaaatgccg tttttggaaa cttggatatc ttatttgcta taggagttgc aatcggtttt
     2341 gtcaaaaata aggacaaagg aatacctgca cttactggtt ttttagcgat tgctaccttg
     2401 aaaaaaggct tagaaattat ggacccagaa gtgaatatgg ggatttttgg tggaattatt
     2461 tccggtttag tagcggcatg gacgtataat cgctttaaag aacaaaaatt accagtagtt
     2521 ttttcttatt ttgcgggcga aaaattccca ttgactatgg tgatgattct acaaacaatc
     2581 gcagctgttt ttttgggctt tatctggccg ctcatccaaa ttgggattga taattttgca
     2641 aaactgctcg ttgatatggg agcattaggt gtggggatat ttatgttttt gaatcgtttg
     2701 ctgatcccct ttggactgca ccatgtgttg aatacgtata tttattacga tttaggaagc
     2761 tatacatctc ctagcaagga agttttcaga ggagagatga cacgctttat caacggagat
     2821 cctcaggctg gactgttttt atcaggtttt tttgtagtaa tgatgtttgg tataccagct
     2881 atatgtctag ctatctataa agcagcttat aaagaaaata aagagatggt caaaggaatc
     2941 atgggaagcg gtgcaggaac ttcatttatt gcaaatatca ctgagcctgt tgaatttagt
     3001 ttcatgtttc tttctcctat gttgtacgtg atccatgcac tttttgccgg tttagcaggg
     3061 attgtctgtt acttattcaa tatacgaatt ggttttacat tcggagcatg tattgttgat
     3121 tatctcatca attttcgaat cgctaccaat gctattttga ttcttccaat cggaattttc
     3181 ttttttgcct tgtattacgt tactttttat tatttgatca ataagagaaa cattcagaca
     3241 ttaggacgtg aagctaaggc agagtttggc aatgaagtaa ccttagaaga aacagagtta
     3301 gggttagcaa gtaaaaatta tgaatatatg gcaacaaaaa tgctacaagc gtttggagga
     3361 aaagccaata tcctggatgt ctatagttgt aatacacgcc tacgggtaga ggtagtggat
     3421 cccaccatgg tcgaggaaca gcgaatcaag caattaggga taagcgggat cattaaacca
     3481 actgaaaaaa attatcaaat tatcatcggc ttagaagtga cttatgttat ggcagaattc
     3541 aataaattat tagaagaata gaaaggaaaa gatgatgatg aaaaaacaga ttatcactat
     3601 agctggaggc gggagtacgt atacaccagg aatcgttcaa gccattttga acaatgaaaa
     3661 acggttgcca ttatctgaaa tacgtttata tgatattgat gaagaaagaa atatggatat
     3721 gtacttgatt gttaaattta tgctgaaaag aaaaggtttt tcaaatattc gcattcgtgc
     3781 aacggatgat ccaaagcttg cttttacagg atgcgatttt gttttctcac aaattcgtgt
     3841 tggaggattg gagatgcgag aaaaggacga aaagattcca cttaaacatg gattagtagg
     3901 acaagaaacg tgtggactcg gaggatttgc ttatgggatg cgctcaatca aaggtttatt
     3961 ggaaattgtc gatcatatac aagactatgc ccccaaagca tggattttga attatacaaa
     4021 tccagagtcg atcgtttctg aagctgttcg tcgtaaatat ccgaatgtaa aaatgattaa
     4081 tgcctgtgat atgactattt caattgaaga aacgattgca gtgaattacg gttatgatcg
     4141 taagaattgg atcgttacgt attatggttt aaaccacttt ggctggtata catcgattta
     4201 tgataaagaa ttgcaacgag aaattatgcc agagattatt gaaaaactaa taacaaaaga
     4261 aatgcaagtc gccgatttta atataggaga taaaacttgg caaaaaacct ttcaaatgat
     4321 gtcagtcatc acgaaaaatt ttccaagtaa tatcccgaat aattacttag agtattactt
     4381 atatcctgat atggtcgttg agcatactga caaagagtat acacgtgcta atatggtcat
     4441 ggatggacga gagaaaaata ccaaagaaat ggctgataaa atacgccgcg gtattgtgga
     4501 agaagtattg aactttaatt ttggggagca tggacaatat atcgtcgata tagctatttc
     4561 cttgttgaat gatgatcgtc gtcgctttat gctaatcgtt cctaatcaag gggcgattcc
     4621 gaatctacgg tcagatgcag ttgttgaaat accggcatat gttggtgcaa ctggtgtgga
     4681 gccaattaca ctaagaaaac caatcagtga ttttcataaa ggattgatgg aggcacaagt
     4741 tgcagtagag aagttactag tagatgctta ctttgaaggt tcttatcaaa aagctttaca
     4801 agcatttaca ttgaatcaaa ccgtaccaaa tgccagagtc gcaaaaaaaa tactagatga
     4861 aatgatcaaa gtaaacaaaa aatattggcc agcattaaag tagaggtgat aaaaagtgca
     4921 tttctttaaa acaagaaaca ttctaaaagc tgtagctagt ggtcaaatta ttccattaac
     4981 agcagtaaaa gacgaggtct tttcttcaaa aatgatagga gttggatacg ctataacgaa
     5041 tcatgatgga tatatccatg cgccagttaa aggaataatc gataatatat ttcctacccg
     5101 acatgccata acgatcgaaa gtgaatctgg cagaaaaata ctgatccata tgggtttaga
     5161 tacagttgaa ctcgaaggaa gtccgtttca gatacgggtt gaaagtaacc aggaggtaaa
     5221 agaagggaca ctgttagcta ttatagattt gaagaagttg gagcaagcac aaaaagatac
     5281 ggtgatcatt gtagtttctc caggcatttt tgaaggaaat ttattaaaac aaaaccaaga
     5341 agtaaagatt aaagatagtt gttttgaatt ttagtgtacg atgaaaaaag ttaaataggg
     5401 tgatatttat gggtgaattt acaaaatttt tttgaaatga tatgtttcaa tcactagatt
     5461 taatcatttt gatttttgtt aattatcgaa tttttttgtt gctcctatga tttagaaaac
     5521 ttattttca
//
LOCUS       DAFCLG010000141         5485 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFCLG010000141 DAFCLG010000000
VERSION     DAFCLG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.143"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..436)
                     /locus_tag="LND56_002485"
     CDS             complement(<1..436)
                     /locus_tag="LND56_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK6744355.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGL"
     gene            621..3062
                     /locus_tag="LND56_002486"
     CDS             621..3062
                     /locus_tag="LND56_002486"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBK6744356.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(3392..4096)
                     /locus_tag="LND56_002487"
     CDS             complement(3392..4096)
                     /locus_tag="LND56_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744357.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(4283..4528)
                     /locus_tag="LND56_002488"
     CDS             complement(4283..4528)
                     /locus_tag="LND56_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744358.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4545..5396)
                     /locus_tag="LND56_002489"
     CDS             complement(4545..5396)
                     /locus_tag="LND56_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6744359.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
BASE COUNT         1680 a         1006 c         1140 g         1659 t
ORIGIN      
        1 ccaagccgta acgagcaata tcacttgcta tgactagtac ttttcgttct ggatgatttt
       61 tgacatattc tttcgccatg tgcaaagctg cggtgccacc gtagcaagcc tcttttactt
      121 caaaagaacg agcaaaagat tggattttca gtaaatggtg gataattacc gcactcgctt
      181 ttgaatgatc aatcccggat tctgttccga cgatgaccat gtcgattagc tcacggtctt
      241 tttctgttac gattttgctg gcagcatttg ctcctagtgt aatgatgtct tcatttgcac
      301 gattgactgc catttggtct tgaccaatcc ctatatggta ttttgcagga tcatccccac
      361 ggctttctgc caactctgtc atatctaaat ataaattagg aataaaaaag gaaagacgat
      421 caatccctat tttcatttca acacctcttt acttatgata aaaaacttac tttactaagg
      481 tatcataaaa agattaaaag aaatataaaa aagagaatat ttaaggattg ttaaaattta
      541 atcttagaaa aaaataactg gaacaagagc tatttgtttt tacagcaaag aatggtatat
      601 ttttgtagga ggtggcttac ttgaaagaag tcgttatgat agatgctgca agaacgccga
      661 taggaaagta tcgcggaagt ttaagtccat ttactgcagt tgaattaggc acacttgtga
      721 ctaaaggatt attagataaa acaaaactga aaaaagataa aatcgatcaa gtaatatttg
      781 gaaatgtcct gcaagctggt aatggtcaaa atgttgccag acagattgct ttgaacagcg
      841 gtctccctgt agatgtccca gcaatgacga tcaatgaagt ttgcggatct ggtatgaaag
      901 cagtgatctt agcacgccag ctgatccaat taggtgaagc agaacttgtg atagccggcg
      961 gaacagagag tatgtctcaa gcacctatgc tgaaacagta tcagtcagaa acaaatgaat
     1021 atggtgaacc aatttccagt atggtcaacg acggattgac tgacgcattt tcaaatgcac
     1081 atatgggatt aaccgcagag aatgttgcaa cacaattttc tgtgagcaga gaagagcagg
     1141 atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc tgtcgaagcc ggtgtatttt
     1201 ctgaggagat catcccagtc aagatttctg atgaagacgt gttatctgag gatgaagcag
     1261 ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg tacagtattc tcagaagaag
     1321 gaactgtaac agcaggaaat gcttccccgt tgaatgacgg tgcctctgtg gtgatccttg
     1381 catccaaaga atacgcagaa aataataatc tgccttattt agcaaccatc aaagaagtag
     1441 cggaggtcgg tattgaccca agtatcatgg gaatcgctcc aatcaaagcc attcaaaaat
     1501 tgacagatag atcaggaatg aatttatcaa caatcgatct ttttgagatc aatgaagcat
     1561 ttgcagcttc ttctatcgta gttagccaag aactgcagtt agatgaagaa aaggtcaata
     1621 tatatggagg agcaatcgca ttaggccatc caattggtgc aagcggtgca cgtatcttga
     1681 ctacattagc ttacggattg cttcgtgaac agaagagata cggtattgct tcattatgta
     1741 ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa tatggagcaa acacataagg
     1801 atgtgcaaaa aaaaaagttt tatcagctta cgccatctga acggcgcagt caattaatag
     1861 aaaaaaatgt attgacacaa gaaactgcgt tgatttttca ggaacaaaca ctttctgaag
     1921 agctctccga tcacatgatc gaaaaccaag tgagtgaagt ggaaatccca atggggatcg
     1981 cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc gatggcaaca gaggaaccat
     2041 ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg aaatatctgc gcagaaacac
     2101 ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa atcggagtat caagcagtga
     2161 tcaatgcagt taatcatcga aaagaagagc tgattctttg tgcaaatgaa agttatcctt
     2221 caatcgtgaa acgcgggggc ggtgttcaag atatctctac tcgggaattc atggggagtt
     2281 tccatgcata cttatctatt gattttttag tggatgtaaa agatgcaatg ggcgctaaca
     2341 tgatcaactc gattcttgag agtgtggcaa acaagttacg ggagtggttc cctgaagaag
     2401 agattctatt cagtatttta agtaattttg ctacagaatc attggcttct gcctgttgtg
     2461 aaatcccatt cgaacgctta gggagaaaca aagaaatcgg tgaacaaatt gccaaaaaaa
     2521 tccaacaggc aggggaatat gcgaagcttg acccttaccg tgcagcaacg cacaacaaag
     2581 ggatcatgaa tggtatagaa gctgtagtgg cagctacagg aaatgataca cgtgcggtta
     2641 gcgcttcgat ccacgcctac gcggcaagaa atggattata ccaaggatta acagactggc
     2701 agatcaaagg ggataaacta gttggaaaac taacggtgcc tttggctgtt gccacagtag
     2761 gcggagcttc caatatcctg ccaaaagcaa aagcatcttt agctatgtta gatattgatt
     2821 cagccaagga actagcacaa gtaattgctg cagtagggtt agctcaaaac ctggctgctt
     2881 tgcgtgcatt agttacagaa ggaatccaaa aaggccacat gggacttcaa gctcgttcat
     2941 tagcgatctc aattggggct attggtgagg aaatcgagca agttgcgaaa aaattacgtg
     3001 aagcagaaaa gatgaaccag cagacagcta tacaaatatt ggaaaaaatc cgggaaaaat
     3061 aaaaaagcaa tatctttaga agaataaagc aagatttaca gttattttat ttactgcaga
     3121 tcttgctttt ttctctatca gaaaatatgt ttattattag aattttttat agttaaggtt
     3181 aggcatctta agaaatcagg caactccaag atatcttcag gtagaagaga agtcgcctaa
     3241 cattgtagga tcataggtga tttgttggac aggtcaagct gatatttgag cctgatgtta
     3301 gaaggaattt ctttttactt gctgtagaaa ttattatatg actaaaaagg ctgggatttt
     3361 aaatcccagc cttttatgac aaatagaatt tttatcgatc agtaatcgct gtcgctgcaa
     3421 attgttcgat caaaggaatc aaagtcaatt tgactttgta tttccgttcg agtgcttttt
     3481 cgataacgaa ctcattttca gtagtcatag aaatagttgc taatttacga ggttcatcag
     3541 catttttttc attaataact acttcatgtt cattttgttc tgtgtaaaat ttaggatact
     3601 cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc attcgtatca gttgagcgtg
     3661 tattttcagt tgtgtccgct tttggttttt ccgttttttc ttctggcaca ctgactgttt
     3721 cttctgtaac tggtgtcgca acagcttgcg cagttggttc atcgtttttc aatcgttgat
     3781 cattccagta agacacgatt tcaataagaa gattgtcacg gaatcgttcg aaaatcccgt
     3841 tttctcctga acgaattctc tttaatacag tattgaagtt gtcatcgatt ggcacgttga
     3901 tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt taagatttga cggtttttgg
     3961 gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa gctctcttct tctagagaac
     4021 ggaataattg tcctcttaca ccatccaatg aaggacggaa ttgatttaat aatttttgtt
     4081 tctgtttttt cttcatatgt tctctccttc gctcataccc tttcgattat aagaaaaaaa
     4141 cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat acgcaaatta caacagaaaa
     4201 atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa tggaacggat tatgtatata
     4261 tacataatcc gttccattct ttctattttt tcggtatcaa ggcaaaaaca ggtagttcag
     4321 gtgaataaat gacttcatgt tcgtgccagg gtttgaccat tagcgaatga tcgaaccgtt
     4381 ttttcagttc ttcgatgatt tgatcatgtg tccagtttct tgcaggaaaa tgttggattt
     4441 tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt ctgggtaact tcttccgaga
     4501 gagaattcaa gcgttcgatt ggatccataa aatcaccttt cgacttaaaa ttgggtagta
     4561 tctgggtctt ttgctttgcc gactacacca tctaactgat cgatttgttt catttcttct
     4621 tctgttaatt caaaatcaaa taattcagta ttttctttga ttctgcttgg tgtcactgac
     4681 ttaggaagag gaaggaattc gtgttgcagc gaccaacgca aagcaacttg tgcaatcgtc
     4741 ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat cgaagatttt accagtgcct
     4801 aatggactat atgcttcgag gatcatatca tgtttttttg catactcaac tacagttgtc
     4861 tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg gcataatttt tgctgttttt
     4921 gctagtgtat ctagatggtg gggtaagaaa ttgctcacac cgattgcttt gattttacct
     4981 gcttcataca gttcttcaaa tgctcgccat gtatctgcat ttgcttgttc ccaattatca
     5041 cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat ctaaataatc tgtttggagc
     5101 ttcttcaatg attcttcaaa agaagacatc acaagttcat agctgtggtt tgcattccat
     5161 aattttgttg tcaagaatat ctcttctcta ggaattccac tttccttaat cgcttggccg
     5221 acgctttctt cattcttgta tccttgggca gtatcgatat ggcgataacc tgcagccagc
     5281 gcttctttaa cagaggaaac agcgacatcc ccatcaggtg tttgccatgt gccgaatcct
     5341 actaccggga tttcgtaacc atttgataga cgataagtgc ttgttaatga attcatactt
     5401 tcagctcctt ttttattcgt tcaactataa gtgtagcgca tttttcgaaa aaaagaatcc
     5461 attttgtttc tactcttgaa atttt
//
LOCUS       DAFCLG010000142         5412 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFCLG010000142 DAFCLG010000000
VERSION     DAFCLG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.144"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(152..550)
                     /locus_tag="LND56_002490"
     CDS             complement(152..550)
                     /locus_tag="LND56_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK6744360.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="LND56_002491"
     CDS             complement(547..1320)
                     /locus_tag="LND56_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6744361.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="LND56_002492"
     CDS             complement(1317..2000)
                     /locus_tag="LND56_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6744362.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="LND56_002493"
     CDS             complement(2167..3054)
                     /locus_tag="LND56_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6744363.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="LND56_002494"
     CDS             complement(3051..3920)
                     /locus_tag="LND56_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6744364.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="LND56_002495"
     CDS             complement(4057..5343)
                     /locus_tag="LND56_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6744365.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1665 a         1209 c          902 g         1636 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aa
//
LOCUS       DAFCLG010000143         5192 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFCLG010000143 DAFCLG010000000
VERSION     DAFCLG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.145"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..748
                     /locus_tag="LND56_002496"
     CDS             137..748
                     /locus_tag="LND56_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6744366.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="LND56_002497"
     CDS             821..1750
                     /locus_tag="LND56_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK6744367.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="LND56_002498"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="LND56_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="LND56_002499"
     CDS             complement(2693..3871)
                     /locus_tag="LND56_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6744368.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="LND56_002500"
     CDS             3969..4067
                     /locus_tag="LND56_002500"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744369.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="LND56_002501"
     CDS             4098..4499
                     /locus_tag="LND56_002501"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744370.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5192)
                     /locus_tag="LND56_002502"
     CDS             complement(4706..>5192)
                     /locus_tag="LND56_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6744371.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1642 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta aa
//
LOCUS       DAFCLG010000144         5079 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFCLG010000144 DAFCLG010000000
VERSION     DAFCLG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.146"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..1479
                     /locus_tag="LND56_002503"
     CDS             205..1479
                     /locus_tag="LND56_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6744372.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1533..2960
                     /locus_tag="LND56_002504"
     CDS             1533..2960
                     /locus_tag="LND56_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBK6744373.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2973..3845
                     /locus_tag="LND56_002505"
     CDS             2973..3845
                     /locus_tag="LND56_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6744374.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3940..4935
                     /locus_tag="LND56_002506"
     CDS             3940..4935
                     /locus_tag="LND56_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6744375.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1753 a          699 c          991 g         1636 t
ORIGIN      
        1 ataaaaaata gtgccattaa aaaagaaaag cgacacacca ataaaagtca tatcctgtga
       61 cattatatgg cattatccaa atagaataac cggttgacat gtaagcggat ttcttttata
      121 attttaggag tacaagtcct tttctaaaaa tgttaaccgg ttatcatgaa aggggataca
      181 aatatatcgt tggaggataa aaatatgtca aaagttaacg tatttaaaaa tcaatcttat
      241 ttacaaagtt cagctacatt attactattt tttgcttctt ggggtgtttg gtggtcattt
      301 tttcaacttt ggctaacatc tgaatcaaat ggtttagggt tatctggcag tgctgtagga
      361 acagtattct cggcaaattc gttagttacc ttaattttga tgtttattta tggaacatta
      421 caagataaat tgtatattaa acgaaattta ttaatttttg cttctgtatt agcgacactt
      481 gttggaccat tttttatatg gatatatggg ccattgctag ataacaattt taatttaggc
      541 attattatgg gagcgctatt tttgtcagct ggatatttag cttctgtagg agtttttgaa
      601 gctgtgtcag aaaggtttag tcgtttattt ggctttgaat atggacaagc aagggcgtgg
      661 ggatcatttg gttatgcctt ggtagcgctt ttggcaggat ttttatttgt aaaaaatcct
      721 catttaaact tttgggcggg atctttcttt ggttctttac tattgttaaa tttattattt
      781 tggaacccta aagttgaacg ggaagcaaat caaaatttta atcaagaaca agctgaatca
      841 aatagtattc cttctttaaa agaaatgttt gatctaatga aactgcctca attatggacg
      901 ataatcatct ttattgtttt tacatggaca ttttatacgg tattcgatca acaaatgttt
      961 ccgggatttt atactggttt gttttcaaca tcagctaatg gtgaaaaaat atatgggaca
     1021 ttgaatgcta ttcaagtatt ttgtgaagcg ttaatgatgg gaattgttcc aatcattatg
     1081 agaaaattag gggttcgaaa tactttgtta ttaggtgtaa ccattatgtg tgtacgaatt
     1141 ggattgtgcg ggtttgcctc gacaccatta tctgtttcat gcataaaaat gttgcatgct
     1201 ttagaagtac cattatttac attaccaatg tttcgctatt ttacacttca ttttgataca
     1261 aagctatcag caaccctcta tatgatagga tttcagatag ctgctcaaat tgggcaagtg
     1321 attttatcaa caccattggg aatattaaga gacaacgttg gctatcaacc aacatttaaa
     1381 attatttctc ttattgtatt actagcaggc atatatgcat tctttattct taaacaagat
     1441 gatagagatg ttcaagggga tccatttatt cgaggataat ggaaaccaac agaataaaaa
     1501 acaattttat ctagaaagtg agcgaatata ttatgacaaa aagagctata gaattaacaa
     1561 atacacgtta tcgtttggga tatcatgtgt ctgcaccatc aggatggatt aatgatccaa
     1621 atggattttg ctattttgat gggtattatc atgtgtttta tcaacatcat ccatatagtg
     1681 ctgaatgggg accaatgcac tgggcacatg ctagaagtaa ggatttagtt cattgggaat
     1741 ctttaccact tgctttgact cctggagatc aagaagatga aggtggttgt ttttcaggaa
     1801 gcgctattga aaaaaatggt gtgttgtatc tgttctatac aggacatcac tattatggag
     1861 acaatgatcc agatcatttt tggcaaaatc aaaacatggc gtatagtacg gatggcattc
     1921 actttactaa atatgaaaaa aatcctgtaa tagcaaaagc accagaagat aacacacatc
     1981 atttcagaga tccaaaagta tggaaacacg atgaaaaata ttacatggtt ttgggtagtc
     2041 aagaaaaaga tggattaggt cgtgctattg tttatcagtc gttagattta ttaaattggg
     2101 attacattgg accaatttct aaagcgaagg aagtacttac agaaggattt atgtgggaat
     2161 gcccagactt ttttgaatta aatggaaaat ctattctttt attatcccct cagggaattg
     2221 aagcaaatgg aaaagattat ttaaatctat atgaaacagg ctactttatc ggtgattatg
     2281 attatgagac agcaaaattc attagaggaa atttttatga attagataag ggacacgatt
     2341 tctatgcaac acaaacaact ttatctccag atggtagacg tattgttatg gcctggatgg
     2401 atatgtggga aagccctatg cctgaaaaag tagacggatg ggcaggagca ttaacgcttc
     2461 ctcgagaatt agagttaatt ggagatcgtt tgtatatgaa acctgtgaga gaattagaac
     2521 aacttcgagt tggcagcggc gttgaaacga cagttaattt ggcaggagaa ataataatcg
     2581 ctcaagatgc ttcattcaaa gaagctctaa ttgatgtact attgactaaa tcaaaaaatc
     2641 aagaagtcaa attttcatta aaaacaaaag atgaagagtt aatcaatatt ttttattcta
     2701 aggataaaaa tgaatgtacc ttaactcgta acggcaaaaa tgatccaagg tatggaacca
     2761 ttcagatgtg tgatagatta tcacttcgag tgtttatcga taaaagttcg gcagagattt
     2821 ttattaatga tggcgagctt gtttttacag agcgttatta tacagaaaat caaccaagta
     2881 tgcatttgag tgttagtgaa gaagcttttg tctcgagtac aatttatcat ctaaaaaaag
     2941 atgcaatttc ttacgagtaa ggaggtttta tgatgttaat tggtagcata gaagcaggtg
     3001 ggactaaatt tgtatgtgcc gtgggagacg aggattaccg agtacaagac caaataattt
     3061 gtccaacgac cacccccagg gaaacattga aaaaaactgt tgactatttt aaacagtttg
     3121 atataaatgc attaggtatt gcatcatttg gtccaataga aattcgaaaa aattcaccaa
     3181 aatcaggata tattacgtct acaccaaagg tgggatggcg aaatgttaat gtgttaggtt
     3241 atttacaaga tgagctgaat gttccaatgt cttggacaac ggatgtgaac ggatcagctt
     3301 atggagagta catcatgtcc actttatcga atgaaaaaat aaattcatta gtttattaca
     3361 ccgttggtac tggcgttggt ggtggagctg ttgttgacgg aaaactatta ggatcgtttg
     3421 gtcattcaga aatgggccat acgtttgtaa aacgccatcc tgatgatatt gattttaaag
     3481 gaatttgtcc ttatcatggt gattgtttag aaggcttagt tgcgggacca acgtttgaag
     3541 cacgttttgg aaaaaagggt gaagacgttt ctttaacgga tcctatttgg gatgtaatgg
     3601 cctattatct agcacaagct gtgattcaaa caacattgat tatacgaccg gataaaatag
     3661 tatttggtgg aagcgtagta aatgaaaaat tgttagaaaa aattcgtgtt aatgttaata
     3721 aattattgga tgattacgtg gaaataccac cgttaagtaa atatattacg cgtcctttaa
     3781 ttaaaaataa tggatcggca acactaggaa actttgcttt gggcttgaat caattacaag
     3841 aataaaacat agaaaaaata tcgattatca ggtatagtag ttctatagag ttcttttttt
     3901 cgatagttaa ttattgcatt atttaggaga gacaagagaa tgcgcccaaa attagaagat
     3961 gttgccaaaa gagcaaatgt atcaaaaaca actgtttcac gtgtattaaa caatcgtggc
     4021 tatttaagtc aaaaaacgat tgataatgtt tataaggcta tagaagaact caattatcaa
     4081 ccgaacgttg tagcgcgtca attatttcaa aagaaaacta acattgtcgg gttactattt
     4141 cctacagtag ccaatccttt ttttagtgaa ctcgttgagg cattggaaaa aaagttatat
     4201 gaaattggtt ataaggtatt aataggcaat tcgatgaata ataaagaaaa ggaaacaaat
     4261 tatttaaatc agttattgtc tgatcaagta gatggtttaa ttgttggtac acataaccaa
     4321 ggaattcagg aatataaata tcagaattta ccgattgttg caattgatcg agtgatgaat
     4381 gaagatattc cagttgttga atccgataac tacaatggtg gaaaacttgc aaccaagtta
     4441 ttaattgcac aaggtgcaaa aaatattatt catacaaacg gtccaattga cttacagact
     4501 ccggctaatc gtcggagact agcctatgaa gatactatga aagcatatca gttaattcca
     4561 cgaactgtga cattagattt caatatttct tatgtaaaga aaaaacagat ttttttccaa
     4621 atgtttgagg attatcctaa aatagatggt atttttgctt caaatgacat cgatgcggcc
     4681 ctaatattac aagtagcaaa agaaaagggg ttaaatgtac cagctgattt attagtagtc
     4741 ggatatgatg gtactttaat gactcggagt atattaccag atctgacaac agtaatacaa
     4801 ccaattaatg atatagcaga cacagctgtt gctattttga tgaaacgaat aaataaagaa
     4861 gaaacaaaaa aggaatatat tttacctgtc acattatgga tggggaagac atcagtaaag
     4921 aaaaagaaca attaaattta taaagactat caatgataat ttcattatta attcgtgata
     4981 taaaaaattg ttaaatctct tataatagac tatgatacaa aatcatgctt ttaatctgtg
     5041 tagatgttgc caaatatttg aaagacatag aacttgtca
//
LOCUS       DAFCLG010000145         4995 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFCLG010000145 DAFCLG010000000
VERSION     DAFCLG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.147"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(221..1831)
                     /locus_tag="LND56_002507"
     CDS             complement(221..1831)
                     /locus_tag="LND56_002507"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBK6744376.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(3024..4364)
                     /gene="glnA"
                     /locus_tag="LND56_002508"
     CDS             complement(3024..4364)
                     /gene="glnA"
                     /locus_tag="LND56_002508"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBK6744377.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4392..4784)
                     /locus_tag="LND56_002509"
     CDS             complement(4392..4784)
                     /locus_tag="LND56_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6744378.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1485 a         1066 c          810 g         1634 t
ORIGIN      
        1 caaacatttc ggtagttttc tactaaaatc aatgaaaatg aaaattttca agaagttttt
       61 tgcaaaaaat aaaatatcca atagcgcttt aatcgaaaaa agtcttttgc tttctatctt
      121 ttttcctaca aataataaca aatcagaaaa agagaaaagt gacaagaaaa ttgcttatcc
      181 ccttctcttg ctacttttct ctttctatca agttcaaaaa ttattttgtt tcgtgatctg
      241 ctaatgatgc tccgacaaat gctttgatta atttttgcgg gcgatttgga cgagaaatca
      301 attctgggtg gaattggcat cccacaaaga attttttctc tggcagctct acgatttcta
      361 ctaaacggtt atctggtgag acacctgaaa agactagccc ttgttcttca aacaattgac
      421 gatatttgtt attgaactca tagcggtgac ggtgacgttc ttgcacaaca tcggctccat
      481 catatgcttc agccgtcttc gttccttttt tgattttaca tgggtataat ccaaggcgta
      541 aagttccacc caaattctcg atgttttcct gatcagccat taaatcaata atattgtttg
      601 ctgtatctgg attcgtttca gctgatgacg catcttctaa tccaacgacg ttacgtgcaa
      661 attcgacaca agccatctgc ataccaagac aaatacctaa aaacggaacg tcattttcgc
      721 gagcataacg aatcgcttcg attttccctt ctactccgcg atcaccaaat ccaccaggaa
      781 ccaagattcc atctgcgtct ttcaaacgtt cttctacatt ttctctcgtt aattcttgtg
      841 ctttgatcca atcgatctcg atgtctgaat tataggcgaa accagaatgt ttcaatgctt
      901 caactactga gatatatgca tctggtaact cgacatattt accgacaagc gcaatccgta
      961 cttttttctt caagttcaag acacgttctt ctaattcttt ccattctgtc atgtctgctt
     1021 ctggtgtatc tagttttaaa tggtcgcaaa caatttgatc cattccttgt gcttgtaaaa
     1081 ctaatgggat ggagtaaagt gtatctacat ctggcgactc gataactgct tctggttcga
     1141 catcacaaaa ttgtgccaat ttgtttttca cgctttgaga aacaggttgc tctgtacgta
     1201 ccaccaaaat attcggttgg atacctaaac cacgtagctc ttttacactg tgctgcgttg
     1261 gtttagtttt catttctcca gcagctttca agtaaggaat caatgttgta tggatataca
     1321 taacattatc tgtaccgaca tctgctttca tttgacgaag tgcttcaagg aatggcaaag
     1381 attctatatc ccctactgtt ccccctactt ctgtaatgat cacatctgaa tcagtcattt
     1441 tagctgctcg catgattttt tctttgatct catttgtaat atgaggaata acttgaacag
     1501 ttgctcccaa gtattcgccc ttacgttctt tacgcaaaac ttcagaatag atttttcctg
     1561 tcgtcacgtt ggagtatttg ttcaaattga tgtcaataaa acgttcgtag tggcccagat
     1621 ccaaatctgt ttcagcacca tcatctgtta cgaatacttc tccgtgctga tatgggctca
     1681 tcgtccccgg atccacgttg atgtatggat caaatttttg gatagttact tttaagccgc
     1741 ggtttttcaa taagcgtcct aaagaagctg caacaatccc ttttccgatt gatgaaacga
     1801 caccgccggt cacaaaaata tattttgtca taagttcaaa aactcctttt taatttatgt
     1861 gcaggaaaag tctcagacaa taaacaaagc tccctattct tcaataagaa tagggagccg
     1921 aaatttctgt aaacttctga ccctctaaaa gggtgcccaa ggtgtataat acaagctgac
     1981 taagggaaag tcaagtactc aaatgaatac tatccgctta caattttttc tttttcgata
     2041 tcctttgaga aattatttct taagtccttt atgagtgcaa aaaaaattta acccacataa
     2101 taagcttgat ataaacatag ctaacgttaa acctaagcaa atcagaaaat cgttactgaa
     2161 aaattttaag tgaaataata gataaaaaaa taaaacaaac attatgatag ataatatccc
     2221 acctaacaaa gaactacttt ttcctacttg cagcaacagt ttatatagct tatcgtagaa
     2281 acaatctttt tctttcaccc ttatttacct cctttacgta aaaaacgtag tccacagttc
     2341 tcggattttt ggcatatctt ttctattaaa taatatcaaa aacaaaacaa ttccactaat
     2401 tttttcaaag atgacctttt tttcaacaaa agtgccttgt agggattcat tgaaggtaac
     2461 gaatcacata agtaaaaaat aaggagatgc gacaaaagtc gtgcctcctt atttctaaat
     2521 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccatca aacatgagaa
     2581 gctgtttttg aaattatttc ttgtgattcg gagttaaatg gtaggctcac aacctctgga
     2641 taaacggtat tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa
     2701 agccattttc acaaaatttt tcttaatact cgaaactaaa cgtctttttc aaaacctctt
     2761 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt tcagccaatt caaaaacaag
     2821 aaaagctgtt tttgcttggc tattcttatt acttgttgca gggcagcttt ttcacaacct
     2881 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
     2941 tgaggaacta tttttccgtt agtttctcta atactcaggt caaaacgctg atttcacagc
     3001 ctctaattca cggtattcct gttttaataa agttctagat attgttctct ttcccactcg
     3061 gaaacagttt gtctgaatgc cgcccattcg agacgttttg cttcaacaaa atttgagaag
     3121 atatgttgtc ctaatgcgtc aatcattaca ttatccttac gcaattcttt gatagcatta
     3181 tggatagttg aaggtaagtc ctggatatgt gcagcttctc tttcttcctc atccattaca
     3241 taaatgttac ggtcaacagc tgctggcggt gtgatctgat tacggatacc gtctagtcct
     3301 gcttgcaaca atactgccat tgccaagtat gggtttgcag atggatcaac tgaccgcaat
     3361 tccaaacgtg tggataaacc gcgagattct ggtacacgta ctaatggcga acgattacgg
     3421 ccactccatg cgacataaac aggcgcttca taacctggaa cgagacgttt ataagaattg
     3481 actgttggat tgcaaacggc tgtgtaggca cgagcatgtt tcagcaaacc acctaagaaa
     3541 tgataagctg tttcacttag ttccaattca ccgtctttat catagaaaac atttccttca
     3601 tcattgaaca aagacatatt gcaatgcata ccagaaccat tgatcccaaa taacggtttc
     3661 ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg ttttgacaac gagcttgaag
     3721 gtttggatat tatcacaagc atctaccaca tctgcatatt taaagtcaat ttcatgctgt
     3781 cctggcgcta cttcatgatg tgaagcttcc acttcaaagc ctagactttc taattccaac
     3841 acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg caaaatcaaa ataaccacct
     3901 ttatcattca aggtcgttgt gatcccaccg tcttcgtcta atttaaataa gaagaattct
     3961 ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc gcatatcttt caatgctcgt
     4021 ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat ccggattata gatatcacaa
     4081 attaaacgag caacttttcc atgagcactt tcccacggga agatcatcca agtggagaga
     4141 tctggataca aatacatatc gctttcttca attcgaacaa atccttcaat cgaagaaccg
     4201 tcaaacatca ttttattttc taatactttg tcgatctggc tcactggaac ttctacattt
     4261 ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa atcgtacgtt ttcttcctca
     4321 atgattcgtt tgatttcttc acctgttatc tgtttcttta ccatggtatc cctcttcctt
     4381 ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt gcgactgata atgacctgtt
     4441 ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa tttgtcgaac gtcatgatct
     4501 gtcaacggct tcttgttttt tgcttcttgt tttttcgcct gtgccatttc gtatacacgt
     4561 ttgatcccag ccatattcaa tccctctgaa agatagtctt taatctcaag caacatatca
     4621 atatcattca gcgaatacat acgtcggttt ccttcgcttc gttccggatg aactaaatct
     4681 tgttcttcat aatagcgaat ttgccgagca gacaaatcag tcagcttcat aactgtacca
     4741 attggaaata cggacataga tcggcgcaat tccttttctc ccatcagacg tcccctttcg
     4801 ttttataagt tggctaaagt gtaccaatta aaaaaaatat tgtcaactgc atatgttagg
     4861 ttttctaaca tgagatctct attttttata aaataacaaa aaaataagaa cacaaaaaag
     4921 gtatctagtt gattttcgta ccagatacct tttatttttt attatactgt actatccttt
     4981 attagtgttt ttatt
//
LOCUS       DAFCLG010000146         4995 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFCLG010000146 DAFCLG010000000
VERSION     DAFCLG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.148"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="LND56_002510"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="LND56_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK6744379.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="LND56_002511"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="LND56_002511"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBK6744380.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="LND56_002512"
     CDS             2591..3376
                     /locus_tag="LND56_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6744381.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="LND56_002513"
     CDS             3389..4240
                     /locus_tag="LND56_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBK6744382.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="LND56_002514"
     CDS             complement(4364..4816)
                     /locus_tag="LND56_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744383.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1690 a          817 c         1039 g         1449 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctga
//
LOCUS       DAFCLG010000147         4835 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFCLG010000147 DAFCLG010000000
VERSION     DAFCLG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.149"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(175..786)
                     /locus_tag="LND56_002515"
     CDS             complement(175..786)
                     /locus_tag="LND56_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6744384.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(909..1034)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1116..1832)
                     /locus_tag="LND56_002516"
     CDS             complement(1116..1832)
                     /locus_tag="LND56_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6744385.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1838..2407)
                     /gene="scpB"
                     /locus_tag="LND56_002517"
     CDS             complement(1838..2407)
                     /gene="scpB"
                     /locus_tag="LND56_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBK6744386.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2391..3188)
                     /locus_tag="LND56_002518"
     CDS             complement(2391..3188)
                     /locus_tag="LND56_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBK6744387.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3158..3529)
                     /locus_tag="LND56_002519"
     CDS             complement(3158..3529)
                     /locus_tag="LND56_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6744388.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3548..4435)
                     /gene="xerD"
                     /locus_tag="LND56_002520"
     CDS             complement(3548..4435)
                     /gene="xerD"
                     /locus_tag="LND56_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK6744389.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4531..>4835)
                     /locus_tag="LND56_002521"
     CDS             complement(4531..>4835)
                     /locus_tag="LND56_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6744390.1"
                     /translation="IGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHH
                     LLCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
BASE COUNT         1337 a         1047 c          830 g         1621 t
ORIGIN      
        1 aaacattttc gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt
       61 tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa acgtttacat tattgcatgt
      121 cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt gatctttttg atatttattt
      181 gactgctgta tgacgttgtt cgaactgatg ttgttttcga cttaaccatg gaagaagttg
      241 cgcatgcagc actaaaaaga cagcactcac gatgaatcct ttgatcaagt tgaaaggaac
      301 gatcccaatc agtacatagt ttgctaaaga catgcccagc atctgtgttg cgttcagccc
      361 taaaaacatt aagtaaagag gcgtaataac gaaataattt gcgatactca taaaaatagt
      421 tagagcgatg gttcctgtaa tgacacctaa aaatttgttt cttcctttat gctgtttctt
      481 tacactaaag aaataataaa tcgggaatgt aaagatcgtt gttgataaga aactagcagt
      541 gtctcctacc aaattatccg gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa
      601 gaacgctgtt gcaactccgg ccgctggacc gaataagaac atgctgatca ataccgagat
      661 atcactaaaa tcgatcttca aaaaactaaa agccggcatg atcggaaaag caaaatactg
      721 taaaactaat gcgatcgcag caaacatcgc tactgcaacc attttctgta cacgggtgtt
      781 cttcatttga aatcctcctg ttaggactcc tcttcagcga actttttgac gtttctctcc
      841 aaatgaaaaa actctatttt ccagggaaaa tagagttaga gttatttgat caagcgtcgt
      901 caaataagct ctatcttctt tatccagact atactgtcgg tatcggaatt tcaccgattc
      961 agctattttt ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc
     1021 ccctgaagac gaaccttata taatttttgt ttacatattc attatacata tctattcaaa
     1081 aaacgcaaca tctaacactt ataaaaagta agtgttcatt ttgagtggtt cagcaaactg
     1141 ctgacttctt ttttgttcag ttcacggtaa tccccaggac gtaggccttg taaagtcaga
     1201 tctccgtatt tttcacgttt tagtttttgc acaggaagac caactgcttg aagcatattt
     1261 ttcacttgat gattacgtcc ttcgtggatc gtcaattcca cgatactgtg gtttgtctta
     1321 ggatccactg ataaaatttg gaatttagct ggagatgttt ttcgtccttc gatctttatt
     1381 ccttttgtca aaggtgcaag catcgatttt gtagcgatcc cttttacctt tgcaacgtaa
     1441 actttatcca cttcatgttt tggatgtgtc aggcgctgag caaaatctcc atcatttgtc
     1501 agtagcaaga tccccgaagt atcgtaatcc agtcgaccta caggatagat acgttctttt
     1561 accatcggca aaagatccgt aacaactttt cttcctttgt catctgcaac agcagaaata
     1621 actcctttag gtttgtacaa taaatagtaa ccgtattctt cttgataaat tgggacttca
     1681 tccacttcca cagtgtcatg tttcccaact tttatcccta attcccttac aacttgtccg
     1741 tttactttta cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga agtgatgcca
     1801 gcgtgggcaa ttactttctg caatctctcc attacaatta ttctccttta ttcattccat
     1861 caaagaatag atctggtgac acgtcttctt caagtgaaat ttccatttct tgtgtatcag
     1921 gcagttcttc taatgatttc aaaccaaaat aatctaagaa gtagccagtt gttccatata
     1981 atatcggccg acctggtcct tctactctac ctttttcttc aatcagttta tgggcagcta
     2041 gacgctgtac cgctcctgaa acctgaactc ctcgaatctg ctcgatttca gctcttgtga
     2101 ttggctgttt ataagcgatg attgctaagg tttcgacggc ggcttgagat aatgtgtttg
     2161 atgtcccttg cgcatatcgc ttaagtaagg gagccagctc ttttttagtc gttaatacat
     2221 aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg taagattctt
     2281 ttaattcttt cactaaactt gtgacttctg cttcgctgag atttaacagt ccgcttaatt
     2341 cttcattact gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt
     2401 tcgtcatgtt cggtatctcc tactgctgga taaagcataa ttgattcata gttttctttt
     2461 tgttgcgcaa tgatctgccc ttttttcatc aattctagca aagccataaa agtagtgaca
     2521 acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt
     2581 tttatacgct cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc
     2641 gttttttcca ctttttgaga aaatttctta cgttctaaga ttttctgcat ggcacggatc
     2701 aaatcgattt ttttgacttg tcctcgcttc aacggctttt cagcatccgc taacccatcg
     2761 atgttcatag gttcttttgt gtaataaaga cttcgttcct ctgctttttc tgtcagtctt
     2821 tcagctgcat atttatattt acggtattct aaaagctgag tgaccaattc ttctcgagga
     2881 tcttcttccc ataattcttc atcgtctgtg acgaattctt ggacaggaag caatgtcttg
     2941 cttttgattg ccatcaatgt agcagccatc actaagtatt ctcctgcaac agctagctcg
     3001 agtgttttca ttgtatggat ataattcata tattgctccg tgatcgtagc gatcggtata
     3061 tcatatatat cgatttccat ttgctgaatc agatgaagca gcagatcgag aggaccctcg
     3121 aatacatcta atttaatatt gatctccgtc aactcattca ctctttcttc tttttctttg
     3181 ctagccattt tccaatgtaa ggctctgtga tttcagttgc acggatggag acatccgggt
     3241 acttttcctg taattcatct aacatttgaa agcccatatt ctcaccgcga taagacggat
     3301 tcaaagaaat gtcatggatg atcagctgat cgttcgttgc ccagtaaata ccaataatcc
     3361 cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt
     3421 cgtacgtatc aatctccttc aacaaagaag ttttacctgt cagtttctta tggaaactca
     3481 atagtcccat agcaatttta cggttttctt tccggtaatg tgttaacatc ttctcacctt
     3541 cctatgacta agcccttgga aaatgctgct tataaacatc cgtcattctt ttcttcgtga
     3601 tatgtgtata aatctgagtc gtagagatat ccgcgtgacc taacaattcc tgtactgtcc
     3661 gcaaatcagc tccattttct agcagatgtg tggcaaagct atgccgcagc gtatgtggtg
     3721 ttacttcttt gtaaatccca gcctctcgta ctaactgctt cagatttttc cagattcctt
     3781 gccgagaaag acctttccca tgattattga caaatacatg catttcagat gcgtccttta
     3841 caagtaatgg acgtgcttcg tctaagtagc gttccagcca ttggatcgca taatcaccta
     3901 aaggaatgat ccgctcttta tctccttttc caactgtctg taccagtcca agagaaagat
     3961 gaagatctcc caatttcaat ccaatcaatt cactgacgcg catcccagta gcatacatga
     4021 cttccaagat tgcacgatct cttatcccta acgtttttgt cgtatcaggt gcttcaatca
     4081 gcttttctac ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat
     4141 cgatatgttg catcggatca tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc
     4201 ttctgagact tgagatcatc cgagtaacag ttgctgtcgc ttttttttct tcatgcaact
     4261 tctccagaaa acggatgact aaaaagcgat cgacatcctg ccatgaagca attttctgct
     4321 cctgtagaaa atgcaagtac tgttccaaat cacgttcata gctttgtcgt gtattggcgg
     4381 acaatccccg ttcaatattc aagtagtgga gataatcgtt gatttgctct ttcataataa
     4441 tccccctcat gcctctctat catatcagat accgcttcat tctgatagtc tttttccata
     4501 atctgagcaa agaaaaagcc gtctagacac tcagacggac tggtttcttt tttctttttc
     4561 atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta aaaaatgata
     4621 acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt cctcgatcgt
     4681 gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt ttctcaaatc
     4741 ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt ctgtcaagat
     4801 ttcaagggtt cgataaacag tagcaagtcc aattt
//
LOCUS       DAFCLG010000148         4823 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFCLG010000148 DAFCLG010000000
VERSION     DAFCLG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.150"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..695
                     /locus_tag="LND56_002522"
     CDS             24..695
                     /locus_tag="LND56_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6744391.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="LND56_002523"
     CDS             754..1194
                     /locus_tag="LND56_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744392.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="LND56_002524"
     CDS             1206..1874
                     /locus_tag="LND56_002524"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6744393.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="LND56_002525"
     CDS             1938..2561
                     /locus_tag="LND56_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBK6744394.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="LND56_002526"
     CDS             2617..3003
                     /locus_tag="LND56_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBK6744395.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="LND56_002527"
     CDS             3216..4607
                     /locus_tag="LND56_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBK6744396.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1608 a          797 c         1024 g         1394 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tat
//
LOCUS       DAFCLG010000149         4811 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFCLG010000149 DAFCLG010000000
VERSION     DAFCLG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.151"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            209..307
                     /locus_tag="LND56_002528"
                     /pseudo
     CDS             209..307
                     /locus_tag="LND56_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(530..760)
                     /locus_tag="LND56_002529"
     CDS             complement(530..760)
                     /locus_tag="LND56_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBK6744397.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(990..1808)
                     /locus_tag="LND56_002530"
     CDS             complement(990..1808)
                     /locus_tag="LND56_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6744398.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1969..2658
                     /locus_tag="LND56_002531"
     CDS             1969..2658
                     /locus_tag="LND56_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK6744399.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2672..4174
                     /locus_tag="LND56_002532"
     CDS             2672..4174
                     /locus_tag="LND56_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6744400.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4187..4663
                     /locus_tag="LND56_002533"
     CDS             4187..4663
                     /locus_tag="LND56_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6744401.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1609 a          701 c          859 g         1642 t
ORIGIN      
        1 aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc ctcacgggtg
       61 tatgggttac tgatagactt gattttaact ggtgtcagaa acttatgtcc agaaagggtg
      121 atgaaatttt ggcaatttac ggctattcaa gaattagcac aagcaaccaa gattacaaaa
      181 cacaaattca aacattagaa aatgctggcg cagaaataat cttcctagaa aatcactgga
      241 ttatcgaaca cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt
      301 aatttgacaa ttaagagtat atttacttca gatggtaagg caacaaaata aaatacaata
      361 ggcagatgaa ctaataaata aaatctgtat taaatacaga taaattaata taaaaatcaa
      421 aaggatcata aacttttaac aagtgatgaa atgggtggaa attgattctt atagacagtt
      481 tgtctcataa cattgaagta ttttgcaatt ttagcattga aacacttttt taatcttcaa
      541 cgttgttctt ataaacaaga tgattgggaa tcttttgaat taacactgta tagcttagaa
      601 caccctttat agaaaacatt cgcttagcaa tcttaataat tccttgtgcc cgatgtcgtt
      661 tagattcagt aggttcctgc tcatcgatca tggaaaaaat ttgaaggatc agatccttaa
      721 taaatttatt taagagagga tgatccattg cttcatccat actcgataaa ctagtgataa
      781 tgagctacac gtttttcttt caagttgatc aatagcttcc acgataaaaa tatctttaga
      841 taaaatcaag aaccgtagga actacaggga tatttggtca ataagagcca ccgctattag
      901 taaaaaataa gctatcaagt atgctctcat cccaagaatt gcctacttca atcgttaaaa
      961 accgttaggt ttagctaagt gggggtagtt cactcaatac ttttatcaat aatagagcgc
     1021 aaaacttttt ctcttaattc aactgaatca atattattgt tttgttcata accatgaact
     1081 aatagatcac aaatataaag ttgtgatatt tttccagcta aagatccacc attaagaaat
     1141 tcttcaatgg ctgtttgtaa aactaagtct gcagactttc ctataggtga atgaatatag
     1201 ttagttatag caataatttt tgcgccattt tttttagcta ttttcaatga atcataagta
     1261 tctttcgttt tcccagataa agaaaaaatg attactaaat cattaactgt taataaagaa
     1321 gcgacttggg cttgaaaatg agggtctagt acagcttttg cttgaactcc tacgcgaaga
     1381 aacatatttt ctaaatcaag actagtattt ccactagatc ccactccaaa tatatatatg
     1441 ttttttgatt gagtgatcat tttaatggct tcattcaatt tttcttcgtc taaaagccta
     1501 atagtggaat agagagcttc tgttaaacta ttagcaattt catcatagta ctttacacta
     1561 gtcggatgtt cttttttttg actaaagtct tcctttgcaa tttcaatttt taaatcagaa
     1621 aaaccagaaa aaccaatttt ttgacaaaag cgaataatag tagcatcgcc aacttttgtg
     1681 tttttcttaa tatcactcat cgtactataa ataatagttt tcccagaatt taaaataaaa
     1741 tcagcgactt ttttttcaga cttcgttaaa aatggataat tttctttaat taaatagata
     1801 taactcatac aaactcctct acaatttcaa cttttctcca ttatactcca aaataataaa
     1861 aaaaactcct aaatactcca tttttatctt tacttttatg aaagcggatg ctatattgtt
     1921 tttggagtta ttatccaaaa ataataaaaa attggagcgt ttaggataat ggataaaaag
     1981 aggtttttag atcttatttc aaaaggtttg attgtttcat gtcaagcatt acctggtgaa
     2041 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
     2101 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
     2161 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
     2221 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
     2281 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
     2341 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
     2401 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
     2461 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
     2521 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
     2581 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
     2641 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
     2701 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
     2761 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
     2821 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
     2881 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
     2941 tggtggagta gcttatttag tatatactgc aactattagt gggtttcttg aacttttttc
     3001 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     3061 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     3121 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     3181 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     3241 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     3301 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     3361 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     3421 ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat
     3481 gtttgggtta ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa
     3541 aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga
     3601 accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt
     3661 agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc
     3721 aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg
     3781 gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt
     3841 ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc
     3901 tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc
     3961 tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc
     4021 tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt
     4081 cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca
     4141 agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt
     4201 caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac
     4261 agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc
     4321 tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa
     4381 acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga
     4441 tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac
     4501 tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac
     4561 ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg
     4621 tcgaattgct catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa
     4681 gctataaaat aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt
     4741 tttctttctg ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc
     4801 gtaatttaaa g
//
LOCUS       DAFCLG010000150         4804 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFCLG010000150 DAFCLG010000000
VERSION     DAFCLG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.152"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..411
                     /locus_tag="LND56_002534"
     CDS             <1..411
                     /locus_tag="LND56_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744402.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="LND56_002535"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="LND56_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK6744403.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="LND56_002536"
     CDS             2446..3708
                     /locus_tag="LND56_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBK6744404.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4804
                     /locus_tag="LND56_002537"
     CDS             3953..>4804
                     /locus_tag="LND56_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBK6744405.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1659 a          665 c          979 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaagaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata atgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaa
//
LOCUS       DAFCLG010000151         4798 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFCLG010000151 DAFCLG010000000
VERSION     DAFCLG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.153"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="LND56_002538"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="LND56_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBK6744406.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="LND56_002539"
     CDS             complement(2066..3064)
                     /locus_tag="LND56_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBK6744407.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="LND56_002540"
     CDS             complement(3290..3943)
                     /locus_tag="LND56_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBK6744408.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="LND56_002541"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="LND56_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6744409.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1407 a         1007 c          744 g         1640 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa ttttttta
//
LOCUS       DAFCLG010000152         4778 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFCLG010000152 DAFCLG010000000
VERSION     DAFCLG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.154"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            348..1571
                     /locus_tag="LND56_002542"
     CDS             348..1571
                     /locus_tag="LND56_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBK6744410.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1606..2373
                     /locus_tag="LND56_002543"
     CDS             1606..2373
                     /locus_tag="LND56_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6744411.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2565..2963
                     /locus_tag="LND56_002544"
     CDS             2565..2963
                     /locus_tag="LND56_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK6744412.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3270..3644)
                     /locus_tag="LND56_002545"
     CDS             complement(3270..3644)
                     /locus_tag="LND56_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744413.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3666..4043)
                     /locus_tag="LND56_002546"
     CDS             complement(3666..4043)
                     /locus_tag="LND56_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744414.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(4033..4428)
                     /locus_tag="LND56_002547"
     CDS             complement(4033..4428)
                     /locus_tag="LND56_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK6744415.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4495..4680)
                     /locus_tag="LND56_002548"
     CDS             complement(4495..4680)
                     /locus_tag="LND56_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBK6744416.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
BASE COUNT         1646 a          707 c          819 g         1606 t
ORIGIN      
        1 gatgcgaaca tggacaaaaa gcgatgtgaa tgtggacaaa aagcgatgca aacgtggaca
       61 aaaagcgatg tggtattccg aatatatcca aaaaaagaaa aagtatgtga atttatttct
      121 aaaaactaga aaaatatttg ttttttaatt ttggtaattt ttataatttc gtattttatt
      181 gaaaaggatt tttattttga aaatgttcta aaatagcgat ttctgctgta ttttcaatga
      241 aatttgattg gaaaaaataa aatgtgtttt actgacattg agttatgtta taaatttacg
      301 atacatttct tattgtaacg tatcgaaaac aggtctatac ttattcaatg gaatcgtacc
      361 agttccaagt ttttaagctc gtaccaagct tagaaagaag aggcaatcgt atgaaaataa
      421 cagaaaagaa tgtaaacccg aaatttgaaa ctaccgaaag caatctaaaa aagtttgatt
      481 taactgttac tttatcgaat caacttgtag atagcattgt tgaaatggat gctatgccac
      541 aaaaaattta tctttacgct ataggacatc ttgaccctaa aaatccgcct tttaataata
      601 ccgtcaaagt atctaaaaag gctttatttg cgttgatgtc ttctagttca tcttcttcaa
      661 aacattcaag attaaaacaa gttattgaaa aattgcatca gcaatctgtc attaaaattc
      721 aaagaactaa acctaacggt aagactgaat acaaagtcat ctcaccgcta gaaattactg
      781 aattcaatga tttttctgat gcagtatcta ttaaatttag tagttcaatt atgcctttat
      841 taacgaactt agaaaatcat tttacgcaat atacatttga tcaagtagtt aacttgacaa
      901 gcaagtatgc gatcagtttt tttcaattat tcactaaaga ttttaatctg tatgaagcaa
      961 atagagataa cttaacatgg agtacaaaat cacttgatag attaaaaaat cctgttttct
     1021 ccattgcaga tttacgaaag aaatttagta ttgagaaaaa atataaagct tttcgagact
     1081 ttgaaagacg tgtaattaaa gaaccgattg cccaactgaa taaatacact gattttaaaa
     1141 ttgactatga gcttatcaag aagggtcata cttatacaaa cataaagttc ttgcttacac
     1201 gtaccatgtc agttccggta ccttataagc aagaacaaag agaccctgct tatttaaaag
     1261 gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg aatagcaaat
     1321 acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc gatcttaaac
     1381 ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc aaagatagag
     1441 gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag gattacacaa
     1501 taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct tatgtgaacc
     1561 tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa aaaagatgaa
     1621 ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga tttaaagtcg
     1681 tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa gaaaacaggc
     1741 gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca aaatcttgaa
     1801 acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga aatttcgaat
     1861 ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat tcattgggat
     1921 ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt aattgtctca
     1981 atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca aaaaaaatat
     2041 agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat cgccaaaaat
     2101 aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat agttaaaatt
     2161 cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga tagacgtgtt
     2221 ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga ttttgctaaa
     2281 gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag tgagtttgat
     2341 gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac tcgtttgaag
     2401 atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta atctccttta
     2461 gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct tttttttgaa
     2521 acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa tacttttttt
     2581 gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat tttccagact
     2641 tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa gtattggaag
     2701 gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg cctgttcaag
     2761 agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta aaagctaata
     2821 cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg actgtggaac
     2881 actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct ttgaatgatt
     2941 tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa aaacagcttg
     3001 ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa aagctctcag
     3061 aagccttctg agagctttta aattgctatg tattgtactg catctgctca aattgatttt
     3121 cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata tgtattgagt
     3181 aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat gtgttaggat
     3241 aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt tttatcggta
     3301 ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat ccctgcatcg
     3361 ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat caaaatggtg
     3421 tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga cttataaaaa
     3481 agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta tttccaaaga
     3541 tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc tgctaattga
     3601 tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct cttctcctgt
     3661 ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc ggtttgaaat
     3721 tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga ttcacttaat
     3781 gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt atagcagaat
     3841 tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa gttattatcc
     3901 tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc agttgtatct
     3961 tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc tttcataggt
     4021 tcggtctcct ttttactttt caagcaactc ttcttctagt gctttttgaa gtagttgaga
     4081 gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac taacattttt
     4141 tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt ggatgattac
     4201 taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag gaaattctgc
     4261 atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat atgcttcttc
     4321 aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt tataagcttc
     4381 tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt cctcgctcct
     4441 tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag gctgttaaag
     4501 ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat tgtggatcgg
     4561 aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc cagtttgtcc
     4621 agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg tcttaggcat
     4681 agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat caatagaaaa
     4741 agaggatgtt ttaacccgtg gtgctagtta attttttc
//
LOCUS       DAFCLG010000153         4476 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFCLG010000153 DAFCLG010000000
VERSION     DAFCLG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.156"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            322..1053
                     /locus_tag="LND56_002549"
     CDS             322..1053
                     /locus_tag="LND56_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6744417.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1198..3225
                     /locus_tag="LND56_002550"
     CDS             1198..3225
                     /locus_tag="LND56_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK6744418.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3333..4430
                     /locus_tag="LND56_002551"
     CDS             3333..4430
                     /locus_tag="LND56_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6744419.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1573 a          717 c          925 g         1261 t
ORIGIN      
        1 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
       61 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
      121 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
      181 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
      241 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
      301 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
      361 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
      421 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
      481 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
      541 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
      601 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
      661 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
      721 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
      781 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
      841 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
      901 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
      961 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
     1021 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
     1081 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
     1141 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
     1201 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
     1261 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
     1321 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
     1381 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
     1441 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
     1501 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
     1561 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
     1621 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
     1681 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
     1741 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
     1801 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
     1861 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
     1921 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
     1981 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
     2041 caaagaagtc ttacttatga tttggaaggt gtcggtcact cttctcggat tatacctgat
     2101 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
     2161 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
     2221 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
     2281 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
     2341 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
     2401 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
     2461 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
     2521 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
     2581 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
     2641 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
     2701 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
     2761 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
     2821 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
     2881 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
     2941 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
     3001 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
     3061 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
     3121 acagctcatc gtggaatcag aatagatatt cctgtagaaa accaaagaat cggaaccttt
     3181 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
     3241 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
     3301 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
     3361 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
     3421 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
     3481 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
     3541 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
     3601 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
     3661 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
     3721 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
     3781 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
     3841 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
     3901 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
     3961 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
     4021 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
     4081 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
     4141 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
     4201 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
     4261 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
     4321 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
     4381 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
     4441 acaaatagta aatttttatt attgtaggat ggttgg
//
LOCUS       DAFCLG010000154         4437 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFCLG010000154 DAFCLG010000000
VERSION     DAFCLG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.157"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..901)
                     /locus_tag="LND56_002552"
     CDS             complement(<1..901)
                     /locus_tag="LND56_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744420.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEK"
     gene            complement(1136..1411)
                     /locus_tag="LND56_002553"
     CDS             complement(1136..1411)
                     /locus_tag="LND56_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBK6744421.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(1654..2964)
                     /gene="der"
                     /locus_tag="LND56_002554"
     CDS             complement(1654..2964)
                     /gene="der"
                     /locus_tag="LND56_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK6744422.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(3146..4375)
                     /gene="rpsA"
                     /locus_tag="LND56_002555"
     CDS             complement(3146..4375)
                     /gene="rpsA"
                     /locus_tag="LND56_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK6744423.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         1267 a          967 c          740 g         1463 t
ORIGIN      
        1 ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga tagccaagca
       61 agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct tggagattcc
      121 acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt agatggccat
      181 tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg ttttctgcct
      241 ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga gtcgttaatt
      301 gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca caaatcattc
      361 cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc tcgctgattg
      421 tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca cgatttggta
      481 gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc gatacaggga
      541 tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc agatccatga
      601 tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag cgtttgacga
      661 aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca caaatatacc
      721 agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac aaatcataat
      781 agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt agctgactga
      841 ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa tcaagttcca
      901 tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc tagaaaagat
      961 aagagcaaag ctcacctcat aacttgcaaa taaataaaaa ttaaataaaa tacatgtgtt
     1021 ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg
     1081 aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt
     1141 taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt
     1201 cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa
     1261 agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg
     1321 aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa
     1381 ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata
     1441 tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat
     1501 tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt
     1561 taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc
     1621 ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg
     1681 gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata
     1741 agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc
     1801 gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat
     1861 tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt
     1921 catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga
     1981 aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa
     2041 atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga cgataataat
     2101 tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc
     2161 ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat
     2221 cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc
     2281 tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt
     2341 cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga
     2401 ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc
     2461 ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc
     2521 atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc
     2581 gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt tattacttct
     2641 gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac
     2701 acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat
     2761 aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa
     2821 ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga
     2881 gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac
     2941 gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag
     3001 tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat
     3061 tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc
     3121 atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta
     3181 cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg
     3241 gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag
     3301 ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca
     3361 cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag
     3421 agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca
     3481 aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc
     3541 aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag
     3601 acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat
     3661 gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg
     3721 cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg
     3781 aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga
     3841 tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat
     3901 agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta
     3961 caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct
     4021 tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt
     4081 ttgaaagtag atagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat
     4141 ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt
     4201 cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt
     4261 cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt
     4321 tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac
     4381 ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgat
//
LOCUS       DAFCLG010000155         4427 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFCLG010000155 DAFCLG010000000
VERSION     DAFCLG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.158"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..522)
                     /locus_tag="LND56_002556"
     CDS             complement(52..522)
                     /locus_tag="LND56_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBK6744424.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="LND56_002557"
     CDS             complement(681..1139)
                     /locus_tag="LND56_002557"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK6744425.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="LND56_002558"
     CDS             complement(1310..2455)
                     /locus_tag="LND56_002558"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBK6744426.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="LND56_002559"
     CDS             complement(2468..3385)
                     /locus_tag="LND56_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBK6744427.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="LND56_002560"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="LND56_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK6744428.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1367 a          823 c          841 g         1396 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaag
//
LOCUS       DAFCLG010000156         4424 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFCLG010000156 DAFCLG010000000
VERSION     DAFCLG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.159"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="LND56_002561"
     CDS             690..1658
                     /locus_tag="LND56_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6744429.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="LND56_002562"
     CDS             1669..2484
                     /locus_tag="LND56_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBK6744430.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="LND56_002563"
     CDS             2552..3262
                     /locus_tag="LND56_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6744431.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="LND56_002564"
     CDS             3298..>4424
                     /locus_tag="LND56_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK6744432.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DAFCLG010000157         4418 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFCLG010000157 DAFCLG010000000
VERSION     DAFCLG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.160"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..913
                     /locus_tag="LND56_002565"
     CDS             59..913
                     /locus_tag="LND56_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6744433.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="LND56_002566"
     CDS             1010..3259
                     /locus_tag="LND56_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6744434.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="LND56_002567"
     CDS             3262..>4418
                     /locus_tag="LND56_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6744435.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          626 c          807 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga taccggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAFCLG010000158         4289 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFCLG010000158 DAFCLG010000000
VERSION     DAFCLG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.161"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..1346
                     /locus_tag="LND56_002568"
     CDS             45..1346
                     /locus_tag="LND56_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6744436.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1373..1633
                     /locus_tag="LND56_002569"
     CDS             1373..1633
                     /locus_tag="LND56_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6744437.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2632..3867)
                     /locus_tag="LND56_002570"
     CDS             complement(2632..3867)
                     /locus_tag="LND56_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6744438.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1252 a          687 c          882 g         1468 t
ORIGIN      
        1 catgcaatga atggttcata ttttattgaa aaggagtggt tttagtggaa aagctgatga
       61 aatgggtcga agagaaactt gtcccaccaa tggcaaaaat tggtacacag cgtcatttat
      121 tagcaattcg taatggggtt gtttcaacat tgtcattgat tttgattggg acatttttta
      181 tggtctttat caatttaccg tttcctggat ggaatgaatt tattgcccct tattcggcaa
      241 cgattgtttt accctttagg atgacgatgg gattgatggc tatttatgca acttttgtta
      301 tgggttcgga tctagctaaa tcatatggat tagatagtgt gacaggtggt atattatctt
      361 taggaacgtt ctttatgtta caagttcctg taaacgtttt aactcctgaa gaggcacctc
      421 ttggctgggt cttacctatg agttctttgg gagcatctgg gatgtttgcg gggatcttat
      481 ctatgatttt tgcagtggaa gtatatcgtt tctttaaaaa tcgaaatatt actatcaaaa
      541 tgccagaaca agtgccacct gctgttgccc gtagttttga ggcattgatt ccaggtgcag
      601 tcattttaac aattacttgg ctgattcgaa gcgtcgcggg ctttgatgta aacgctgcct
      661 tattatcgtt attcgagcct ttaaccaata ttttagggaa taatttgctt ggtgttttac
      721 taccaatgtt tttgatccat ttgttgtggt catttggtat ccatggcatg agtattgtag
      781 gggctgttgt tcgtccaatg tggctgatca tgttggatga gaatgcaaag gcattggcag
      841 atggtacagc tgccacccaa ttaccctata tcgctcctga acagttttat caatggacgg
      901 taacaattgg tggtgcagga gcaacgatcg tggtatctgt tttgtttata ttcttctgta
      961 aatcaaaatt cttgaaagag gttggtcgtt tttctattat tcctggtatc ttcaatatca
     1021 atgagcccat aatttttgga gctcctatga tgttgaatcc ttacatgttc atcccgttca
     1081 acttagttcc tttagttttg actattatca gttattttgc agtcaaatta gaaatggtca
     1141 atagatttac tgttttgcca acatggacat taccagctcc aatcggcggt tatctttcag
     1201 caggaaatga ctggcgtgtt gttatcttga tcatgatcaa cacattgata gcatttatta
     1261 tctattatcc atttgtaaaa gcttatgata aaaagatgct gtcagatgaa caaagtcgtg
     1321 gagaaaacga agtccagact gtctaagttt ggtttttaga aggtgggaaa caatgtattg
     1381 gctgatttta ttttttgttt ttatcttttt attaacggct agtcacctta tattgaatat
     1441 gctagctaca tatcatattc aaatcaatcg atggatttgg gcactggcat catttttgat
     1501 cgtgattctt ccaaagatca ttgtaccgca tatgaatgtt ttgttttcat gggggacata
     1561 tgtactttgt ggaatttttg ctatcaattt tatgatcgag cagcatagat ggtttgtcac
     1621 atcgaagctt taaatgttct aaaagtggtt gtaacagaag tggttagctt caagaaataa
     1681 gaagaaattt acgaaaattg cttcttaaat ttttgtgaaa tgttggctta tttttcgttt
     1741 ggttgaactt attaccgcag atgcaagctt ttgaccaccg tttattcgga atatgggact
     1801 gtgacaaggc ttttgtcaca gccctttttt acataaagat tattattgtt ttaactgttt
     1861 tggcagaaag aaggaggaaa aaatggaaat tttatcagca atggatttag aattagtaga
     1921 tctactacca aagtgatcaa gtcaaatata gatttgggag cttacaacca tatagtaggg
     1981 gctgcagtat ggtgtatgaa tggtaatatg tatgatgtga gtgtttattc aaatcatgaa
     2041 acgtgtatag aaataattgt gctgggaata gcaatcagtc atggtgaacg tgacttttag
     2101 tgtatcgttg ctgacgacat agaacattct gacatgatct actcaatatg tggggattgc
     2161 tggcagttta tataggagta tgcatcggat tgcgagatca ttgtctcaac agaagaggga
     2221 ggttgattat caaagataaa gtggtaaatc ctacatttga tttagtaatt gttgagggga
     2281 ctagcggcac aaatctctac aaagcgatgc ttctagatct atatgacaca ttttaagaaa
     2341 atgtatttgc ctaattatta tggtctttct tttgaagaaa cgttgagatg atccaaccag
     2401 agaaaggtgt caaagaattt gactaagaat aaatggcaga cgctacctat gctcatttgg
     2461 atagcgtcag atcttttatt ttaaggatga ttttgtgttt tttagacacc gtaatttaag
     2521 taactctaac ttttcaatcc actatttact ttttttgtat tttcactgtc tgggtctcga
     2581 atagcgaaat tactgatgag tctctattaa aaaagctttt tctacttgaa actatattgg
     2641 gtggttgctc aggaaggttt cgatttcttg aattgcttgt tgtggaccat ttattggaaa
     2701 aacggcttgc agcgtgcgtg cctgctgacg aatcttgggt gaagaaactt cgcgcagagc
     2761 aatctgcagt tttctttcag tcactggttt tgtgagggcg attgcgtttc cgtattttac
     2821 acaatagtca atattacagc tttgctcaaa ttgcatcccc acaccgataa acggtttgcc
     2881 ggattcacag gcagtttgga cggtcccttc cccgccgtgg ataaaagaaa aatcaatgaa
     2941 ttcatgcaaa agatgactag acagatattc tgttattaaa agatcttctg agtcagattg
     3001 aatgtcatca agcagatagt caataggtgc aataaccgct agatccggtt gtttttgtaa
     3061 gaagaacaaa attttgcgta acatacgcgg attgcctgaa ctacccattg cgaaatagat
     3121 aatacgtttt ctttcttgtt gctttcgctg tataaaagtt agcacttctt caggcaagga
     3181 agtactgagc ttagcaaaaa ttggaccaac gattcgataa ttttccggca gctgttcttc
     3241 ttccaaaaat agtttaggcg ttgtgatgag atttagatca gcatccatca tttgtaaact
     3301 tgtttttggt aaaggaagca tttctttttg tgcagcgtgt ttgaaggcat tggggatcca
     3361 tcgggtattt actatacgct tttttgtcca gtgccaaagg ggattgctta aagggccgaa
     3421 gaaacgtaac tcgtggggca tcagcgcatg ctctgattga aaaaaaggtt cactcaaagc
     3481 atagggttta acatatacta aaggaatctt tttattgcgg gccgataaaa aaattgtcac
     3541 attcgaacct gtcacaatca gtcgaggctc aaacttagca atcagactct gttcactagt
     3601 gatacgagag gcaaccattt ctttggtaaa agggttttta agtgtacgca actggtcaaa
     3661 ttttatgatt tgttcttctt gttttttcgt aaggatcggc gctagtaaat gatactcaaa
     3721 gccactttct gtgatcaaag gagcaaaagt tttagaaaag ccaaagaata cacagtaata
     3781 acggtctttc atagctttgg caacctcaat cattcgtgtt gtctcagcta aattgaatac
     3841 tgcaggagca aaaagaattg tcttcatgaa atcctcctta ttggaatgag tctatccctt
     3901 cgtttcttac tagtatacta aaaaacacag cgttcgagta gcctatgtat attttgaata
     3961 ttaaactgtc tctcgctgtt ttcacaactt ctaaatttaa attttgtaat caagttgaaa
     4021 ctccaatttt caaaaaaaca cgcaacaatt ttttgatcga tttatatgtt gtgccagata
     4081 aatacgagaa gttgtgtcat taaaaataaa catgagaaca taaaaaaata aaataattat
     4141 attaattgat aggtgtgctg tcgtcattat acacacgtaa gaaagaaata gttatgttta
     4201 aaaatattct tagactttct cgccatgaca attttcacta tgtgttataa ctgtaaacga
     4261 taattattca gataacacaa aaagaaatc
//
LOCUS       DAFCLG010000159         4282 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFCLG010000159 DAFCLG010000000
VERSION     DAFCLG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.162"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="LND56_002571"
     CDS             complement(37..1257)
                     /locus_tag="LND56_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBK6744439.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="LND56_002572"
     CDS             complement(1277..1888)
                     /locus_tag="LND56_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6744440.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="LND56_002573"
     CDS             complement(1931..2863)
                     /locus_tag="LND56_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK6744441.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="LND56_002574"
     CDS             complement(3284..4144)
                     /locus_tag="LND56_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744442.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAFCLG010000160         4243 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFCLG010000160 DAFCLG010000000
VERSION     DAFCLG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.163"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(577..1116)
                     /locus_tag="LND56_002575"
     CDS             complement(577..1116)
                     /locus_tag="LND56_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6744443.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(1132..2055)
                     /locus_tag="LND56_002576"
     CDS             complement(1132..2055)
                     /locus_tag="LND56_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6744444.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(3345..3779)
                     /locus_tag="LND56_002577"
     CDS             complement(3345..3779)
                     /locus_tag="LND56_002577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744445.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1298 a          719 c          640 g         1586 t
ORIGIN      
        1 aaattttcaa aagttgttct aaaaaatttt tcttttttgg aagttactct aacttttttt
       61 gcttgtggtg cttggtatgt tttctccgcc tcccaggttc ttgaccattt tctctcaacg
      121 tggtaaaaat cccacttata agagaaaatg gtcaagaacc tgggaggcgg agaatgccct
      181 taataccaag caccgcaagc ctttcccata ctttattcga tgatcccacg atgatctcat
      241 aatgattcta caatgaccct acgatgatca aaattctcaa aaaaaataaa cgaaatttca
      301 aacgattttt ttgaaatttt gttaaaatca catttgtttt tttatgactt tttcaacaaa
      361 atcgattaga atttattttg tttttccacc aaaaattgag tgagatttca acctatttta
      421 agcaataagt caccatatag tttgatagtt tacaactata aacattgatt gtttacagtt
      481 gtaaactatt ttttctttct cataaaaatt tgagtgatct ccccatcagg tagtacaact
      541 ttttcctcta ctatccgaac taacttctag aaaattttag agattggttc tattagattt
      601 ccaaatttgc caccactttt tttcaataag tggttgtgta ttgtctgctt ttttttcaga
      661 aaaggcagtt gattcggttg ttggagtttc ttctgtataa gttaaatgga gctgttcttg
      721 taggttctca atttgttggt tcgcttgtag agtgagttgt tgttgctggt cgagtaattt
      781 ttgcatttgt tttatttgat tatctttttc ttgtagctgt atatcttttc gatttaactg
      841 tttttctagg aaagaaagga gttttaattc ggaaacttgg ttggtgtttt tatggttttt
      901 ggtttttgat ttttcgccaa ctagttttcg atttaagttt gcgttatcga ctaaaaacat
      961 tgattttata gctttttgac cggtttcgtt aatcacaaac ttgtttccgt tttttgcaaa
     1021 ccaaagtttt cgatttttgt cagaaacttt tttgttaatt gctgttttac taactcctaa
     1081 ttcttccgca agttctttga ttgttttatc tgccataaat actccttaag cttaatcgaa
     1141 taagttttgt aaatttccta gtaaatcttg tttgagtttg tttgaaagtt ctcgtttttc
     1201 tgcctgtttt tcttcagttt gttgttgttg tttaaaatca gcttcatgag tgcctaaggg
     1261 aagatttagt attcgatctt tagcgcgcca tttttcttct cgtgttaatt ctccattgtg
     1321 atcaatatta aataatttta tacgtaaatc ttcttgtttc cctttggaaa aatcgtcagc
     1381 atggttaatt tcagctttcc aagtaaaacg ataaccgata acaggtttac ctcgaccttt
     1441 accatatttt ttacgaatag ttaatccttt aaataaggga gtgagttctt ctttaattgg
     1501 ccttaagata aatttgtcaa tatttgacgg actattccaa taacttttag gaatatctag
     1561 taaatcaaaa aatttttctt tggaaaattc agtccagcct tgagttcgat attgttttaa
     1621 taatcgaaac atagtttttg aatatgtgct atccaaatta gtaaattgct ctagattaaa
     1681 tcttgtccag ttctttaatt gattaaaaag tcctttaaat tctggattaa ctgaaattat
     1741 aacaagttgt ttatctcgta taatgctata ttttgtgaaa gcaacaaatt tagttaaagt
     1801 atttccatca tcattccaag cattaatact taataatttg tcatacgtgc tactaagatc
     1861 ttttataaat tgttccccac gttctgtata accgcttagt tttttcaaat cgtgaaatga
     1921 aaaaataatc tcatctacac ctttttcacg tacacgtgaa gcaattgaaa aaaataagtt
     1981 catttcacga ctattaaatt ttctcagagg aatatgattt aatctatttt catattttac
     2041 aatttcattt gccataagct tctcctactt ttataaagta tgaaataaaa gtaacatact
     2101 ttataaaaat actcaataat gtcggaaaac ctacttgaaa agcgtagaaa acctacttga
     2161 aaagcgtaga aaacctactt gaaaagcgta gaaaacctac ttgaaaaaac tctgaattgc
     2221 ttgggagagt aaggacacag ccgacataaa agagatataa aaagagatat aaaaagagat
     2281 ataaaaagag gggaggctaa ttttcccgcc taaaaaatca ctcctttttt ctctaaacag
     2341 gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct ccctacggtt cgctaaaaag
     2401 gtgtgatcgc tctgctctca cattatactg cgcagtcgcc tccggctcct ttgctaaaag
     2461 ccgttcataa ggaacccttt gagaagctct gagcgttcgt ttaagacatt ttaagacagt
     2521 cctagtataa atcccttatc ttgttcttaa aacgccttac aggcggtcag ggcaagccct
     2581 aacaacccta aaattgtcta aatgccctct aaaattcgat ttaagcactc ttaaacctat
     2641 tcttatatat ttatatactt aggcgtttca aacgtcttaa aacgcaaatt agagcttgat
     2701 aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg gcacacgcca tgacaaagct
     2761 gaaataaact gcttcagctt tgtcatggcg gattcgctca cttgaaaaag tgtttatttc
     2821 gctcgattat tagcatatat tttttagctt ttttgctagc caaattttgc attaatttgc
     2881 tcataagtcc caccctttca gtacgtccta cggacgtatc gttcgtaatg tgaaagtacc
     2941 tttaaaggta ctttctacaa aactcactaa aagggtttgg caagctctat atttgctagc
     3001 caaatacctc cgtatttggt gcaaataacc gcttgtcaaa ggggcgcaag ctcccccttg
     3061 aatccccctc aaaacccacg ctccacaata caaaaagtgg ctagaaaatg ctagccactt
     3121 tttgtaaaat ttagtatgat ttatttttag ctatttagag tttttatttc cgtaaatact
     3181 tctaaaaatt gctaagataa atgctatagg aattagataa agaatacttt tttgtaagct
     3241 tatattgaaa attagcctaa atatgaagaa catagtgatt aaaactatta ttgaaataat
     3301 tacagctaaa atctttttat tcatgagata gtttcacttt ctgattattt tattacataa
     3361 cgagcgtaat cattgtattg atatgatgtt gtattgctaa agtgagcatt atatgttaat
     3421 tgtacacctt ttccttgatt tgcagctctt acaaagacat tcattgcatc aacagcacta
     3481 ctaaaagcat ttgtaataac ccatgttcca actccaccaa taggaataaa agatgcaatt
     3541 tgagcaggag tctttagctt attataattt tttttcaaat ctctacacat tttattagta
     3601 acataatagt tcattttata gcaagtaaat ccacgatgat tatgattata tcgtgaactt
     3661 gcagcttcca cttttatacc tctagcacta aaaggaaaca gatttaacgg agtacttgct
     3721 ccaacaatca taccgaacaa aaatagacaa gtaataattt taataatttt atttttcatt
     3781 gttacttcct ccttagatat aaaatcctag gaaagcgctt tctgtatata tttagaataa
     3841 tcagaaaatt aaatattgtc aagagggttt attttataaa acgtgttatt ttaaaggtta
     3901 gtagacataa tgtacagtat gccaagtagc ccttgtatca accgatacag gggctcactt
     3961 tgtgattttt aattggtcta ggtaaaaagc tctttttaag gcttgatttt ctgcagttgc
     4021 ttctttccgt ttctttgtga atttttacca gtcatttttt actggattgt tatttttcat
     4081 aaaatatgat aaatttttgg tagcgcttta taagcgcgtt gctttttcaa gaataaacac
     4141 tcattatttt ttaactggct gctcggaaga gcagtctttt ttattattta tccgtttctt
     4201 ttacaaacaa ttcttcgtta ggactgttgt aataatttga att
//
LOCUS       DAFCLG010000161         4217 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFCLG010000161 DAFCLG010000000
VERSION     DAFCLG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.164"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..484
                     /locus_tag="LND56_002578"
     CDS             47..484
                     /locus_tag="LND56_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744446.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            477..1172
                     /locus_tag="LND56_002579"
     CDS             477..1172
                     /locus_tag="LND56_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744447.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            1553..1732
                     /locus_tag="LND56_002580"
     CDS             1553..1732
                     /locus_tag="LND56_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744448.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            2073..2336
                     /locus_tag="LND56_002581"
     CDS             2073..2336
                     /locus_tag="LND56_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBK6744449.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2330..2680
                     /locus_tag="LND56_002582"
     CDS             2330..2680
                     /locus_tag="LND56_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6744450.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(2704..3318)
                     /locus_tag="LND56_002583"
     CDS             complement(2704..3318)
                     /locus_tag="LND56_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744451.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            3632..4054
                     /locus_tag="LND56_002584"
     CDS             3632..4054
                     /locus_tag="LND56_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744452.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            4064..>4217
                     /locus_tag="LND56_002585"
     CDS             4064..>4217
                     /locus_tag="LND56_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744453.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDS"
BASE COUNT         1512 a          640 c          776 g         1289 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
       61 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
      121 attcctatac aatgagacac gtgttccaaa aaaacattat aaggaaagat tgtcggtagc
      181 tgtggaagaa atgattgaat cgggtgtcga aatgaatctc attgcgacct actatcgaac
      241 gcttgaagaa ctgaaaaagc aaaatggaca atggtttttc caagccttac tatgcttgga
      301 agcaggcgtc aagccaaaca ctatcaaacc ttctgaatac caagctttag aactaacgta
      361 tgggaaattt gtcgaaacga aaaaagccaa gactgtttca agtgaatggt tggactactt
      421 agaaaatgtc aacaagtatg gcgcatgtta catcatgaaa aaggaggaca acgaaaatga
      481 ataaggtgat ttatcaacta acaacggaag atgtgtcaca ctttttaaca aacaaagaac
      541 atttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa gaaatgaagt
      601 actttattca aagagtcgaa gaacaattgt atttggaaag tgaaacgagt caaacgaagg
      661 ttacagtcag caataattat ttagacgtgt ataaacgtcg tcctgacaca ttaaaaaaag
      721 gagcggagct tctacgacta gaaacagaaa aatatatcat gaatgtttac ctagaaagga
      781 gtgaactggt cgaaaacttt gtcagctaca cgatttgcag gaaacactat tactcagaat
      841 cagaatatta ttcaattttt gagagcatgg aattaatgga agagctaaac agcaaagatg
      901 cgacggtaga cgattattac cttgtattta ctaaattttt gacgtgtggg acggaatcta
      961 tcgtgttaat gggaatggat tatgaactcg ctgtacccga agtatttttg agggaatatg
     1021 tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat tttttagata
     1081 cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat
     1141 atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt gagaatgtgt
     1201 gaaataaaaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc
     1261 caaccgcccc cgccgcttag gtggtgcgaa ataagcgagc aaagcccgaa aacctaagag
     1321 aaagaaggag atttatatga acaaaattat tcccttatta ttagtcctat ttgttggaga
     1381 aatacttatg cgacttagtg cagctgtaca tttaaacctc gcagtatttt tggtaatact
     1441 aatgttttgt ttgatctttt ggcgatttgt attagccctc gtaagtctac tattcaagat
     1501 gattgcagcc atatttttca taggattatt tcgatgaaag gagagagtac caatgccaat
     1561 tgcaatagga aataaacggt tacccgtgac attagatgaa aagagacaaa aagaattgca
     1621 gcaactaaag cagaagtacg gaaaaagtga atctaagatt atgtgtattg cgttagatct
     1681 attgatcgcg caagaaaaag caggatttga tataccagca ctaaaaaagt gacgtcactt
     1741 ttcatttgaa gaaacaaaag tgatagcact taattgttcc tactatttag aatattttct
     1801 atgtcctttt tattccattt tgtatcaata actatgaaat ctttaaagta tccagaagag
     1861 attattttta gatcaatttt tttcttacct gattctaaag attttgtttt cttaaacata
     1921 ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg attcatggaa
     1981 tgttaatcta atttaataga aaaggataat taagtatata cgttttttct tatgtatgtt
     2041 atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata aaaaaatggg
     2101 gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga attcatacag
     2161 aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta attatcgaag
     2221 cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac actgatgaaa
     2281 gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa tggtgagaaa
     2341 acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac acgaacagac
     2401 aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata gtaacgttgt
     2461 tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa atataaactc
     2521 agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga caattgatta
     2581 tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg aggagttatt
     2641 gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca gcagcatgat
     2701 caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt gtctcgtaac
     2761 attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct caacgatgtt
     2821 tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct taggacgtcc
     2881 tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc gtttagattc
     2941 ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt taatgaattt
     3001 atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga tgatcagctg
     3061 cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat ttcttccaag
     3121 tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca gtgcttcttg
     3181 aaaaacaagt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa ttctctctgc
     3241 accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg atgagacacg
     3301 tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa aacaccctaa
     3361 tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag ggtatacctt
     3421 atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt ttaaatggca
     3481 atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca ttaaaaatat
     3541 attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt aagaaaacgt
     3601 tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt tatctttagg
     3661 tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg ctgatagtac
     3721 aaatgaacca actactgtag tagataataa ccaagaatta actcctttag ataggaacgg
     3781 aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt taatagtttc
     3841 tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga tgatttttga
     3901 taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa aaagaaatgg
     3961 ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa acaattggat
     4021 tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag aatttgtgaa
     4081 cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac cgaaagaaga
     4141 atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa aatcaatttc
     4201 taatctagaa gattctg
//
LOCUS       DAFCLG010000162         4194 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFCLG010000162 DAFCLG010000000
VERSION     DAFCLG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.165"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..269
                     /locus_tag="LND56_002586"
     CDS             <1..269
                     /locus_tag="LND56_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744454.1"
                     /translation="LIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIENDFDH
                     ALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            311..733
                     /locus_tag="LND56_002587"
     CDS             311..733
                     /locus_tag="LND56_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744455.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            752..2344
                     /locus_tag="LND56_002588"
     CDS             752..2344
                     /locus_tag="LND56_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744456.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2356..2691
                     /locus_tag="LND56_002589"
     CDS             2356..2691
                     /locus_tag="LND56_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744457.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2838..3032
                     /locus_tag="LND56_002590"
     CDS             2838..3032
                     /locus_tag="LND56_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744458.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            3064..>4194
                     /locus_tag="LND56_002591"
     CDS             3064..>4194
                     /locus_tag="LND56_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744459.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHS"
BASE COUNT         1733 a          618 c          834 g         1009 t
ORIGIN      
        1 tattaattag aaatattgaa gaaaagtata ttaataaaat tgacaaacgt tgtgaagaac
       61 ttacggtcaa gactggcaaa aaatggagtc gaaatcaata cctaaaagtt ttgatcgaaa
      121 atgattttga tcacgctctt ctgaattata aaaaagacca atttgatcgg ttgttagaaa
      181 aatttgtgga tattcaaact tataatacga aaactttaga agaatatatt gcaacaaata
      241 atcaattaat tggtttgtta attgaataat aaaaatagta ttgtgaattt ataaaaggag
      301 gaaatcaatt ttggcaaaag caacaagaaa acatgtatat ctttacgata tgcatatcga
      361 agtacttgat gaaataattt ctactcatga agatatatct aattatagtg aagcagtcag
      421 acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa ataaatccaa ttcaaattaa
      481 attgaatgca atgggaaaag aaatttctat tttaaccgaa atggtttcag agtttgcgga
      541 tcttcatttg aaagatacag gaatactaat tggtacaaat tcaactgttt atcaagaagc
      601 agaagaacgt gtggaaggaa aaattaaatc caatcaaacc aaaaaatatt cttctaagtt
      661 aaagaaacca gtgaatgaat ctgttcatac cagagtagat ccatttgatc caacgaagat
      721 tctaaaaaga taaaagtaga aggagaacgt gatgaataaa tcgcctgcag ttattcttac
      781 ttgtcaattt accttgccca ataaaaaatc tttttcaaca tacattgatt atatgacaag
      841 agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa gaagaacagg aactgcaacg
      901 aatcaagaat gctttagagg atttttatat tcctgaagga gaaacttttt cggaaagtaa
      961 gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa tcgttgatgg aacatggaat
     1021 caattttgac aagttagagg aacaagattt taccaaatat ctttcttata tgacccgtca
     1081 ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa caaaaagaat accaacggtt
     1141 gaaccaagca atgaaaaaat atcaaacaga gccacataaa aaagcttcag gaaatcttcc
     1201 tggagttttt tctatggctt cagacgaagt gaaaggaagt gatctgaagg agatcaaaca
     1261 aatcttccaa agaggacaac aaaaaggctc aatcatttat caagatgtta tctctcacga
     1321 caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa acagatacct tagatgaaga
     1381 aggtttgaaa gcagctggaa gaaaaatgat ggagacatta ttcgagaaag aacaattgaa
     1441 tgaaacaggt tactggatgg caacgattca ccgtaacact aagcatatcc atattcattt
     1501 tgcagttgtt gaaaaagaaa atacgcgtaa accgtatact gagattgaaa atggtgtgag
     1561 ttatatcgta ccgaagggga agagaagaca agcaacatta gacgcaatga aaaccaatta
     1621 tgtacatgag ttagaacgtt tctcttatga aaaggatcga atgatcggta tggagaaacg
     1681 aaatttattg acacgaaaaa gtgatttgcg taacacctta acaaaagtag taaaagaacc
     1741 agcacgttac gatcgacacg ccatgaactt attaaatgaa gtatataaaa ccctacctga
     1801 aaaacgttcg gaatggaatt atggagatga aaatcgaacc aagctttcac catttacaag
     1861 gggaaaactt gatgagttaa ccaaatatgt tttaacaaaa gaacctggat atcaagaata
     1921 tatacagatt tcaaaagcat tgaaagaaga agccaaaagc ctttatgggg aaagcaaaag
     1981 agaaacaaag aatgcagaaa aaaacgccct ctttgattta aaaaagcgca caggaaatgc
     2041 gattttaaca gaactgaaga gacaggatag tcaaataaaa ccattaatag aaagtgtcaa
     2101 tcagatgcaa tctatttaca atgaacgatc ctttcgaaca ctcaaacagt tgccaacaaa
     2161 tgaatacaag tatagttatc aagaattcgt aaagcgtagg aaacaattcc aacaaagagt
     2221 tgtcacgcag cgaagcttgc gtcaattaaa tcgaacaatt aatgcacatg aagaaaaata
     2281 ccacgcaagt aaagcatatg aagagcaaca gcgaagaatc caaatggaac aagaaagaca
     2341 ataggaggaa gagaaatgaa acaatattta gatcaatgga aagtcataga aggatcacta
     2401 aaagaagaac gaatcgaaca acttcctgat tgtttagaaa aagagcattt gtttcagata
     2461 agcgaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagttgt tgagtaccca
     2521 gctactggtg tgtattgttg taatcatgtg aagggtgaaa aatattttat tattcaagaa
     2581 tacgaaggta agttagcgcc ttattatact acttgggaaa taaatgaaga gggaatcaat
     2641 aatttcccat gtgaaagcat tgaagagtct atttctctca cggaatgtta aataaataga
     2701 gacgtaaaag agtcctgaac gtgattgtcc aggactcttt ttcaatgaat tgttttccgc
     2761 caattcactg agtggtgcga ttcaatttta ttatatcaaa agaaagtgag attccaaagt
     2821 gaagggagga agaagaaatg actcagacaa cgattggtgt tgtggttgaa aaaagcaggg
     2881 ataatctcgt ttttgtgaca gaaattcaaa caggtagagc ttttgtagtc acagacaaag
     2941 ctgcaaaagc gtatcaaaat ggcaatattt taacgttaaa tctgactacg aagatgttcg
     3001 ttgatgcagt agaaaattat ccatttgttt agacaaacga tattttacaa ggaggaaacg
     3061 gacatggcag aagaaaaaga accactagat aatagtcggt tgggaaagtc caaaagaaaa
     3121 ttagtacggt tgcaaaacga attaaataat caatttcaaa agctatttga tcaccaaaaa
     3181 ttgacaaatg ggcaaccaat gaatgacaaa cgaaatggtc gttcatggtt taaccagcaa
     3241 gaaaaaatag aaaataaaat acaaagtttg agagaagaga tcaagcaaca agaaaaacag
     3301 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
     3361 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
     3421 ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
     3481 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
     3541 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
     3601 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
     3661 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
     3721 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
     3781 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
     3841 gatcaactta aaacgttgga taaccaatac gctgcagaag caggttatta taaattctat
     3901 tttgaagaag tgaagaacgg agaagtagta gatcagatcc gaatggatct aggggatggc
     3961 gtcaacagta atcaccccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
     4021 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     4081 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat caaacaagaa
     4141 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttct
//
LOCUS       DAFCLG010000163         4174 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFCLG010000163 DAFCLG010000000
VERSION     DAFCLG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.166"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            434..601
                     /locus_tag="LND56_002592"
     CDS             434..601
                     /locus_tag="LND56_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744460.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            675..1901
                     /locus_tag="LND56_002593"
     CDS             675..1901
                     /locus_tag="LND56_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6744461.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2059..2676)
                     /locus_tag="LND56_002594"
     CDS             complement(2059..2676)
                     /locus_tag="LND56_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744462.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            2982..3320
                     /locus_tag="LND56_002595"
     CDS             2982..3320
                     /locus_tag="LND56_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744463.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            3412..4117
                     /locus_tag="LND56_002596"
                     /pseudo
     CDS             3412..4117
                     /locus_tag="LND56_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         1488 a          681 c          693 g         1312 t
ORIGIN      
        1 tattttaaat ttttacatac ctgtttatga aaaactaaat aatctgtcaa aaaaatatga
       61 ttgttcattt ctaaatatgt tttccaattt taaaatcttt tttagcattt ttgtttcctt
      121 ttcatatggt ttttcgttgt taatagtgac taggatgata ttcctatttt gaccattgac
      181 aattaaatat aatttttgaa taacgttccc tgctattcga gctaagaatt caagtacgcc
      241 atgacctatt tagcgataac tacttagaga tttctttttg ttttgggaag acaaaatgcc
      301 atgacaatag cagtagataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      361 aaattcctat gtgcttaccc aagtcaattc aaaaatttta gttcatttat aaggggatga
      421 ttttattaaa tcaatagtct atctaaacaa aaaacaaacc tgtgagtttt taggaattga
      481 taacaatact ttagataaat ggattcgtat atataatttt cccgtaataa aaatagacgg
      541 tataattcgc tttgatgtag aagatatcaa aggctttatg gatacgttca aaaagatttg
      601 aattttactt ttgactgcat atactaaaag tagcgaaata tactgcctta cttttttatt
      661 aaggagtgag gaagttgcct acgaagcttt caaatggaaa atataaaact aatttgagat
      721 atccaaaaaa atttaaagaa attacaggta tcagttcaga aaaattccaa aaaacatttc
      781 caactaggca attggctatt aaagccgaaa atgaaatgaa aaagaaaatc gcaaaagtct
      841 taagagagga gaatgcaaat tcattagagc tcaaaggtca aataaaattc aaggagtttt
      901 ataaatctaa atggcttcct agatatgagt tagggcaaac tactagatcc cataaagctc
      961 caagcgatat aaccatcatt aatacaaaga atttatttcg attgcatatt ctgcctatat
     1021 ttggtgagta tgccatgagc tatcttaatg caaataccga aataatatca gatgaactca
     1081 caaaaaagtc gaaggaatat gcaaacataa aaatcattaa atgctatgtt cgttccatgt
     1141 ttgatattgc agaggttcta aattatattg aatttaatcg tacaacaaaa ataattcaaa
     1201 gtattactgc acctaagaaa gttgctctgg aagaaaaaag aatacgagaa ggaaaacaag
     1261 ctttatcttc gaaagaactc actgattggt tagacgctgt aagagatgat ttcaatgatc
     1321 aattactttc ctttcacgat tacactcttt ttatgcttac tttatatctt ggggatagaa
     1381 agagtgagac ctatgctttg caatggaaat acattgattt tgaaaaccaa acagtccgtc
     1441 ttaaatatgc cttggataaa cacacaaaaa agaaatttac aaaaggtcga aaagatacta
     1501 ttatccaagt tccggaaatt gtaatgacac tattgcaaga atggaaatca gtgcaagctg
     1561 aacaactatt gaagttgaaa attaaacaga cacctgacca atatcttttt agttactcca
     1621 aaccttctgg tgaagtaaat tgtccagtcc atactgatta cttaaattat cgaattaatt
     1681 ctataaaaaa aagacatccc catttggttc atttaactcc tcataaactt cgacatactt
     1741 acgcaacttt agctagacaa ggtggggctg atatgaatca aatttctaac gctttgacac
     1801 attcaggtat ttcaactaca aaaatttatg tgaatacacc tgatttagtt gataaagctg
     1861 tgttcgaagc gtttcagcga ggattacaaa aatctaatta atgtgttcta gaatgtgttc
     1921 taaatataaa aaaaagacac tttccaaata attggaaagc gtcttgaacg ttgatatata
     1981 aaggatatta acgttttgag aattgagatg ctttacgagc tttcttaaga cctggtttct
     2041 tgcaaaatta tattagcctc aactgaaaga tttatttttt tgctccctaa cttcattagt
     2101 aataccatgt ttcattcgat atcttctaaa cgttctacga ttaatccctg ttagctcttc
     2161 aacttcttta tctgtagatc cagtcaaaaa caaattaaat gctaattgaa gtcgcggatc
     2221 attctcacga tattttagcg tacctccctt gtatttccct tttgctttag cgagtgcaat
     2281 tccttgttgt tgcgtttcgc gaatataccg acgttcttct tcggcctggt acttgtatag
     2341 ttcgataatt aagttattaa gtaatcgacg taaattatca tcagtgatac ctgttaacgc
     2401 tggcaaattc aaaacttcca gtgtagcttt cttcagctga attaaattca tcgtttctgt
     2461 tagctcttta ttatttcgtc caagtcgttt taattctgtc acaacgacaa tatcaccttc
     2521 acgtatgaat ttcatcattt cttgaaatcc ttctcgattg gtatctttcc cacttgcttt
     2581 atcactaaaa attttatcac actctgataa taattctaat tgtcggtcta aattttgatc
     2641 taagctgctt actcgagcat acccaatttt cgccataata aaactccttt aaaattctga
     2701 aatccggatc atttttaaat tctgttgccc aactctgttg tcatttttct agataaatgg
     2761 cataaatttt cagcgctaag tgacaaaagc aactaatttt tcatgataga tttttaaata
     2821 tctcaatcta tctccaaaat tagttgcttt tgtagctata gttgactatg aatatgacaa
     2881 ataattagaa aaaactgtgc caattaaata tcaaaatgac aaattgatga atcaaattca
     2941 aattaagggg ggcaaccctg gaagtcttaa atttaccctc aatgaatggt attgaagatg
     3001 aaaatttaag gcgtttgatt aatagccttg tcattgaatt gtacaagtat caagcagaat
     3061 cagaacgaaa aaaaattaag gaacgtcagg cacaaggaat cgaaattgct aagaaaaaag
     3121 gcaaattcaa aggtcggcaa ctgaaattta aaaaaaatga tccacgttta aagcatgctt
     3181 tcgatttgtt tttgaatggt ttatccgata aagaagttga agaacaaact gggatcaatc
     3241 gccgaacgtt tagaaggtat cgagcaagat ataacgtgac agtagatcaa agaaaaaaca
     3301 atgaaaagag ggatagttaa tgagtacggt tattttagct gaaaaacaag ccaggcatta
     3361 gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga gatcaaagac
     3421 ccactattta cagatgaaac gtttatcacc tttgattttg ggcatttagt ggaattagca
     3481 gaaccaggtc attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt
     3541 cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgca
     3601 gaacttctca aaaaggcaaa tacaattatt gttgcaacag atagcgacag agaaggtgaa
     3661 aatatcgcct ggtcgattat ccataaagca aatgcctttt caaaagataa aacatttaaa
     3721 agactatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca aaatttgcaa
     3781 ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg
     3841 ttgatcggca tgaacgcaag ccctttgtat acgttaaatt tacaacagaa gggcgtacaa
     3901 ggtacatttt cactaggacg tgttcaaacg cccaccttat accttatttt tcagcgccag
     3961 gaagctagta taaaagtaaa acaagggtca tttaagggcg ttctaagccc cacacagcgt
     4021 tttaaaaccc aagaggagct tttttgcttt tgtttcttct aaacaagcta aaataggcaa
     4081 tcaagagggg ataattgctg atgttcaaac caaagagatg gataggctac aattaactag
     4141 acagaaaaat taaggtgtgt agactagaag aaaa
//
LOCUS       DAFCLG010000164         4147 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFCLG010000164 DAFCLG010000000
VERSION     DAFCLG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.167"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..388
                     /locus_tag="LND56_002597"
     CDS             50..388
                     /locus_tag="LND56_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744464.1"
                     /translation="MVAILEMRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            392..754
                     /locus_tag="LND56_002598"
     CDS             392..754
                     /locus_tag="LND56_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744465.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            975..2381
                     /locus_tag="LND56_002599"
     CDS             975..2381
                     /locus_tag="LND56_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBK6744466.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            2828..3802
                     /locus_tag="LND56_002600"
     CDS             2828..3802
                     /locus_tag="LND56_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6744467.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEVGAE"
     gene            3799..>4147
                     /locus_tag="LND56_002601"
     CDS             3799..>4147
                     /locus_tag="LND56_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744468.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQ"
BASE COUNT         1445 a          602 c          928 g         1172 t
ORIGIN      
        1 aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat
       61 tctggaaatg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
      121 tgaattgatg tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag
      181 tgaaatgctt gaacgaatta tggatgttct caactcggtt atcaatgctg atttagcaaa
      241 atcttcagtt ttgactaatg aatttgttca taagcattta gcgcaaacaa ctgatgaatc
      301 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
      361 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
      421 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgc caacagaaaa aacgcttgat
      481 tgagaaagaa aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt ttgaaacttt
      541 tgctgatttt gagcggtggc tatcgtcgat ttctgacgat cagcaacacg aaaaatcagt
      601 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga
      661 atctgacggc ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
      721 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
      781 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
      841 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
      901 tgttactctt tgacaaagag taacagaaag gtttacactc tttttattag cgacagccga
      961 aggagggttt taacttgggg ttcagtattt cgttcaaaaa gggaacaaag aatacaagca
     1021 tttctcataa taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata
     1081 ttgatttcac aaaatcggat gaaaatatct atttagaaca aacaaatatt cgtgaaaaat
     1141 atgaagaatt attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata
     1201 gaaaaatcga ggactattta gcaaaggttc gcaaggataa aaaattggaa ccacagcgag
     1261 aatttatcgt gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt
     1321 ctactggcat atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag
     1381 ttgagtattt caaagaattt aaagaacgga atcctaattt atccgtctac aatgctgtta
     1441 ttcataatga cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt
     1501 ataaacgggg tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac
     1561 gagcagataa agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt
     1621 caattgaacg tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa
     1681 tcaaagatgt tcacgaatat aaagaaatca tgagcgagat ttcagaattg cgtcaaacgg
     1741 ccgtaaaaga gcgtgagagc gtttctgatg agttgaggta tatttctagg caaagagaag
     1801 aaaatgagcg agaaaaggct ctggtggctt ctgagagagt gaagattgaa gaagagaaga
     1861 aaaatatgct gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat attgttcctt
     1921 tagaaagttt caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg
     1981 atttgtatca acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg
     2041 agcaatattt acagatgctt gagcaaaata cagaattaaa tgagtatgta gctacattgg
     2101 aagaagagcg tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa
     2161 agctgaaaga agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc
     2221 aaaaatcgat cactcgtttg tctatccgtt ttggtcttgc agaaaaggat atggggcttt
     2281 ctgaggaatt agaagtgtta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac
     2341 agcgtaaatt agagcgttct gaagatgaga tcgagtggta gttacgatgt gcagtcaacg
     2401 gtaccggagg cggtttgtgc cgaggataag gagttgacgg ctaaaccact agcctattaa
     2461 gctgacattt gctaaaaaat ggccagtcca ttttttggcc aacctagcgt tagcgaagtt
     2521 tgggggcgcc cttttcaaag ggtgcggttt tattttttgg atatttgcat agctaaagag
     2581 aagtgctatt attgagttag caaacaaatg aactgaaaaa agagtaaaaa aatagtcgag
     2641 caacgctacc aacgttctcg acctttacgg aaactaatcg cactagctcc gtaacttctt
     2701 aatcattata cgaatatttt tagatttctt caagactaga gattttttgg taatgaattt
     2761 ttaagagtta cgtgaacttt tgaaaattct ttccagaagt ctctttttct ttgaggagga
     2821 acaaaatttg agtaaacgtg gcacgatcgt gaatgagtgg gaaaaactta accgcatttc
     2881 taacaggcgg ttaaagtatc aaagtttgac ccttccctta aaaatgcagt ggtcaattga
     2941 ccgaattacc atagtcggaa aactgaaaga aaatatttat tatcacactc caaatgatgt
     3001 gttgatttta aattttgaac agcttatgcg tttgaacgaa ggcaatggtt atttaaaagc
     3061 tgttgggaat aatgggtggc aattattaga ccaacacgag gaaaatatag cttatattga
     3121 gattcttaaa tttcatgaag gaaaagggcg gattgacttt aatcctaata aaatcaatca
     3181 atttttatca ggttccatga aaaatttcat tcatgattta tttctagaac ctcatttttc
     3241 acgtgcggat attgcttgcg atattgtaga tattcccgat gactttgtta gtcagtatcg
     3301 tgtagttgat cctgtgtctt ttaaaccaat ttatgggcgt tctggtaagt tagaaacggc
     3361 atattggggt agtcgtgcca gtgaacgaca aattcgaatg tacaataaaa aattggaaca
     3421 agaaagaaaa cgtaaaattg ttccgccgga aataacgaca tggtggcgac tagaattgca
     3481 gttacgaagg ggaaaagcaa ctgattggca tgagatggtt catgaaagtt tggatagctt
     3541 tgctagtcca cattattttc cgccaaatac taaagttact gaacggatta tggtgaaagg
     3601 tttgctttat gatcattctg agtggggaaa tctagctaag aatacaaaga aaaaatacag
     3661 ggatttgttg aagcaagaaa gtcaaaatga tgaattgacg aatcatttgc gagagtcatt
     3721 tgcggaatcg gcagacgatt tgaaacgaga attagataca tggctccaag gacttgatgt
     3781 aacggaagta ggtgctgaat gagtaatcca gtagaaaaaa tgaccataaa aaatcttgct
     3841 gacgaattgg gtgtatcgaa aaatacagtg cgcaatcaac tggtcaattc agggtttgac
     3901 cttgctcagt ataaagaaaa tggcattatc atgctagata aagagttgat tggtgttgtt
     3961 cggtcaattt atcggtcaaa aagtgatcaa ttgactggtc aattaccggt caataacaca
     4021 ggcagtgatt ttcctttaga aaaagagaat gagtttttgc gtgatcaatt agctgagaag
     4081 gacaaacata tttctgaatt aacagaaatg attcgtgatc aacaaaaatt gaccctgctt
     4141 cagcaac
//
LOCUS       DAFCLG010000165         4090 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFCLG010000165 DAFCLG010000000
VERSION     DAFCLG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.168"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..151)
                     /locus_tag="LND56_002602"
     CDS             complement(<1..151)
                     /locus_tag="LND56_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6744469.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRW"
     gene            complement(473..694)
                     /locus_tag="LND56_002603"
     CDS             complement(473..694)
                     /locus_tag="LND56_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744470.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(792..1037)
                     /locus_tag="LND56_002604"
     CDS             complement(792..1037)
                     /locus_tag="LND56_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744471.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1277..1891
                     /locus_tag="LND56_002605"
                     /pseudo
     CDS             1277..1891
                     /locus_tag="LND56_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2261..3262)
                     /locus_tag="LND56_002606"
     CDS             complement(2261..3262)
                     /locus_tag="LND56_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBK6744472.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3421..3687)
                     /locus_tag="LND56_002607"
     CDS             complement(3421..3687)
                     /locus_tag="LND56_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744473.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3677..4033)
                     /locus_tag="LND56_002608"
     CDS             complement(3677..4033)
                     /locus_tag="LND56_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6744474.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1294 a          744 c          542 g         1510 t
ORIGIN      
        1 cccaacgata aatcgtagta tgacaaacat ttattccacg atcatataac aattcctgaa
       61 cttcacgata gcttagattg taacgcaggt agtaaccaac agcgacaata atgacgtctt
      121 ttttgaattg tttgccttta aaatgattca ttactctgtc ctctctgtct tttttctcaa
      181 ttttacacta aaatagattt tttggaaaac tttgcaacag aaccgttttt actatatact
      241 tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt cattgggtgg
      301 tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta tgtcctattt
      361 caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt aatatgaagc
      421 tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac cattatagat
      481 cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat gtttcaaagg
      541 tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa gtccaatcaa
      601 tattacggta agaggacatt agattatggt accttgctct ttgttcaact gaagccaatt
      661 cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa gacgctacct
      721 caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata gcaaacaaat
      781 atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct tctattctta
      841 atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc atttattgtt
      901 gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc atcctgatag
      961 ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac ttcttttaca
     1021 acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct ataatattta
     1081 tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt ataaactatt
     1141 taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat agacaaaaat
     1201 ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa acgttataga
     1261 aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg atcaaaatga
     1321 acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta tcgaaaaaca
     1381 atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt ttgtcagaga
     1441 gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg acgagattat
     1501 cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca gtttaccgat
     1561 catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact taatcattta
     1621 aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc aggcacaagg
     1681 aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat acagtgcaga
     1741 caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc ttgagaatgg
     1801 ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa tttaccgaat
     1861 aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt aattattaat
     1921 tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt gattacattt
     1981 atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc cttatttaaa
     2041 ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa gatttgaatt
     2101 ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt acgattgcac
     2161 aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt aaaaaagttt
     2221 atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt aaatctgatt
     2281 catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc ataataacag
     2341 atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc tctgaaatca
     2401 aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt aaaattcccg
     2461 atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc cttaaattgt
     2521 tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa aagatagaat
     2581 tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat tctattggat
     2641 caaaaatatc taagttgtca tcaaaatctt tatcagttcc ggtaactttt ttgttaatta
     2701 ctgaaggtgt cttgtaagaa atagaatcta aatcaaagcg ttttgattct aaattatttt
     2761 gtgctttctc aattaaataa ttcacttttt ttgtgagaaa ccttctgata ttgtctttgc
     2821 tatatgttcc ataaaaaccc ttatcttcta aagagtccct gtacttctta acttctatat
     2881 actcttcagc caaaacttgt ggaactggtt catctaagaa aaaaacccct acctcgcctt
     2941 tgctattttt ataaaagtaa tcaatttctg ctatagttcc cgatctataa ttatcaactg
     3001 gagaacccaa cttatggcca aaaatcgcta ataatatatc actattttct acaatttgtt
     3061 tattaataac actttgccca gagctattat ttatataagt aggaatcgca ttcgtttccc
     3121 atcgaattgg catcaaaatt acttttgtat tatctgtatt ttctatattc catctatata
     3181 tggtttcttc tacttcattc ctctttgctg ctatatcacc tggtgatgct ataaatactt
     3241 ttattagatt tatggttttc atttattgta cctttctctt tatgtctctt taaagtaatt
     3301 tttgtataaa atttattgac ttttacacta taggacaact tttatctatt tagtacaaaa
     3361 aggcccacta aactaattga ttagttagga gcctattaca ggtatatttt caactcgctt
     3421 ttaagcaaat tctactacca gctttttgcc taatgcccta gcgatatcat caagtttatc
     3481 gaaagatacg ttatcccctc tctcaattcg agcaatagta gattgagttg tatgtgcttt
     3541 ttgtgcaagc tccgcttgag ttaggcctgc ttcctcccgt gcatgataaa gagcaaccgc
     3601 agtttcgagt tttcttcctt cacggtcata tgcttctgca aaatctttgt ctttttgctt
     3661 ttcttcaatc aattttttaa tcatcatttg tgaaccactc ctctctaagt tcttttgcat
     3721 gtttaagttc acttattggt gttttctccg tttttttagt aaatccatgg gtaatgacat
     3781 accttccatt gaccacatgg aaatagatag ctctttggat gtttgatcct accttacttc
     3841 ttaattcgta caaattagtg tctaattttt tcacccattt caaccgttga gctactagca
     3901 tcccattctt ttctgtttct tctattgtac gtaaaagctt cgcactatct ttttttggta
     3961 atgtttccaa ccattctaaa aattcatcat gaccattagg tcttttaaat gtttcaaaag
     4021 atattttttc catacccaat tatatcatat attgatttta tattatagta tataacataa
     4081 aaattcgaat
//
LOCUS       DAFCLG010000166         4050 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFCLG010000166 DAFCLG010000000
VERSION     DAFCLG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.169"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..122)
                     /locus_tag="LND56_002609"
     CDS             complement(<1..122)
                     /locus_tag="LND56_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744475.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
     gene            308..1243
                     /locus_tag="LND56_002610"
     CDS             308..1243
                     /locus_tag="LND56_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6744476.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1328..2338)
                     /gene="gap"
                     /locus_tag="LND56_002611"
     CDS             complement(1328..2338)
                     /gene="gap"
                     /locus_tag="LND56_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6744477.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            2641..3948
                     /gene="obgE"
                     /locus_tag="LND56_002612"
     CDS             2641..3948
                     /gene="obgE"
                     /locus_tag="LND56_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBK6744478.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         1267 a          713 c          906 g         1164 t
ORIGIN      
        1 acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac cggcttatta
       61 tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg tttgtaggtc
      121 atttatggtt acactccttt gttttctttc gtcggaaata caatttgagt gtaccataaa
      181 tgccttttta tttttctaac ttaattttac aattcgcggg ttataaaaat gaacattgca
      241 tagtagagat tctttgatta tactaattca aggttatatg aaaaaagaga aatgagatgg
      301 atgatttatg aaactttgga tgaaaatcct tttaggcgtc gctttagctc tgattgttgc
      361 gttagtgttt gcagggaatt atttttataa ttatgctgtt gtgccttctg agaaagactt
      421 tttagacgaa gatacacctg gtacaacaca gacatccaaa gaaacggatg cacataaatg
      481 gtttagttca aaagaaaacc gaagcgattg gaccatcact tcaaaagacg gtctgaaatt
      541 atcagcgatt tatcttcctg ctgaaacgaa aagtgaaaaa acagctcttg tggctcatgg
      601 ctatatggga aacgctgaaa cgatgaccaa ttacgcaaag atgtttcatg atatgggtta
      661 caatgtcctt gttcctgatg caagaggaca tggaaaaagc gaaggggatt acattggatt
      721 tggatggcca gagcgtaaag actatgtaca gtggataaac aaagtgctag aaaacaatgg
      781 aaaatcgcaa gaaatcgttc tatacggtgt cagcatggga gcagcaacag ttatgatgac
      841 tagcggagaa aaactaccag ataatgtgaa agcaatcatt gaagattgcg gttattcttc
      901 cgttcatgat gagcttgctt atcagctaga cgatatgttt agtcttccag catttccgct
      961 tatgcaagta acgagtctag tgacaaaagt ccgtgctggc tactttttcg gtgaagcaaa
     1021 tgcagtggaa caattgaaga aaaaccagcg cccaatgctc tttatacatg gagacgaaga
     1081 tacatttgta ccatttgaaa tgttagataa ggtctatcgt gcaacgaagg gaccaaaaga
     1141 aaagtatgtt gtaaaaggag ccgagcatgc ggaagcatac aagacagatc ctgaaaaata
     1201 tcagcaagta gtccaacaat ttttaagcca atatgttcat tgaacgagtt taaaagtagg
     1261 ggatgaaaag aaagaagcct ttgactggat ccagtcaaag gcttctttta tcgattgatc
     1321 tatcaaatta caagttagcg aatttttcta gtaagcgaat catttggcaa gtaaatccat
     1381 actcattgtc ataccatgca acagttttaa caagttggaa atcaccagca gttgttactt
     1441 ctgtttgcgt tgggtcaaag atcgaacctt gagttgttcc aatgacatcg ctagaaacga
     1501 tttgacggtc atcatacccg aatgaaggat tgtcaactgt gtgttttttg attgcttcgt
     1561 ttacttgatc agcagttact tttgttttca atactgaaac caattcagtt aatgaaccat
     1621 caacgactgg aacacgttgt gcgtgtcctt gtaattttcc ttgtagctca gggataacta
     1681 agccgattgc tttcgcagca ccagtagaat gtgggatagt attgtcagca gctgaacgag
     1741 cagcacgcaa attaccgcct tttacaggtc catctaagag catttgtgtg gaagtgtagg
     1801 catgaactgt tgtcattgta ccaacttcga tcccaaattc attatttaag aaataagcca
     1861 taggtgctaa acagtttgtt gtacatgaac ctgctgagat gattttgtcg ttttcatcta
     1921 atgtatcatc attgacgtta tagacgatgg ttttcatagc accagctggg gcagaaataa
     1981 ctactcgttt gacaccagca tctaaatgcg cttttgcttt ttcttcagat gtataaaaac
     2041 cagtacattc tagtacgata tctacaccgt tttcttttgc ccaagggatt ttgcttgcgt
     2101 cgggttctgc ataaacacga gttgcttcgc catccactac gattgagtct tctgttgcag
     2161 taactgtgcc aggataagta ccatgagtag agtcaaattg caataattgt gctaacatag
     2221 ttggacttgt taagtcgttg attgctacta cttcaatatc atctgagact tctttgatgc
     2281 ggcggaaagc cagacggcca atacgcccaa atccgttaat acctactttt actgtcatat
     2341 caaaagttcc tcctttatta ggggtgctgt gaaacggcat cctcaattaa tacaattaat
     2401 acaattaata cactgttatt ctgctacgca aagaatattt tgtcaaaaaa ttatacttgg
     2461 tagcagaaaa aaggttggaa accttaccaa atgataaaaa aagaagaagg taaacaatct
     2521 ttttgctgaa atgatagaca aaatcagatt tcaccgataa aataagtaag acttttgttt
     2581 attgtggaaa ttcaggcagt caaataaata aatgaataat aggaggactt aattatgtcc
     2641 atgtttttag atcaagtaac gatcgatgtg aaagctggca agggcggaga cggaatggtt
     2701 gcatttcgtc gcgaaaaata cgttcctgac ggcggaccgg ctggcggaga cggtggtcgt
     2761 ggcggcgatg tcatcttgat cgtcgatgaa gggttacgta cattgatgga ttttcggttc
     2821 aatcgccatt tcaaagcgca gccaggtgaa aatgggatga gtaaagggat gcacggtcgt
     2881 ggttcagaag atacctatgt taaagtacca caaggaacca ctgtccgaga tgcggagact
     2941 ggtgctcttt taggagattt aattgaaaat ggtcaaacac tagttgtggc aaaaggtgga
     3001 cgcggtggac gagggaatat ccgttttgcc tctcctcgga atcctgcacc ggaaattgct
     3061 gaaaacggtg aaccaggaca agagcgaaaa atcgaactag aattgaaagt attggcagac
     3121 gttggacttg taggttttcc atctgtcggg aaatcgactt tactatccgt tatttcatct
     3181 gctcgtccaa aaatcggcgc ttatcatttc acgacgctcg tacctaatct aggaatggtt
     3241 actacaagtg atggcagaag ttttgccgca gcagacttgc caggcttgat tgaaggagct
     3301 tcgcaaggtg ttgggctggg tacacaattc cttcgtcata tcgaaagaac acgagtaatt
     3361 ttacatgtga tcgatatgag tgggatggaa ggacgagatc cttatgaaga ctatcttgcc
     3421 atcaacaaag aattatctac ttataactta cgcttgctag agcgtccgca aatcatcgtg
     3481 gcaaacaaaa tggatatgcc ggatgcaccg gaaaatctag tgaaattcaa agaacagctg
     3541 aacaaggaaa aagaagatga atttgctgat gacatcccag tatttccaat ttctggagtg
     3601 actcgacaag gattggacgc gttattgaat gctacagcag atttgttaga agtaacgcca
     3661 gaattcccat tgtacgaaga agaactggaa gaagaaactg tacactatgg attcaatcca
     3721 gaaggtcctg agttccaaat cgatcgtgat tcagatgcaa cgtggatctt atctggtgag
     3781 aagatcgaaa aattattcca aatgacgaat ttcgatcatg atgaaacagt gatgcgtttt
     3841 gctcgtcaat tacgtggtat gggtgtggat gaagctcttc gggcacgcgg tgcgaaagat
     3901 ggcgaccttg tacgtatcgg tgagtttgaa tttgaatttg tcgaataggc aagcaaataa
     3961 aaacaaatac caaaaaatat agattcagat ttaagggagg ttgtgacaga agtgatcagc
     4021 ttcaagaacg ccgtttattc ggaaaaagga
//
LOCUS       DAFCLG010000167         3984 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFCLG010000167 DAFCLG010000000
VERSION     DAFCLG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.170"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            17..148
                     /locus_tag="LND56_002613"
     CDS             17..148
                     /locus_tag="LND56_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBK6744479.1"
                     /translation="MILKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK"
     gene            164..313
                     /locus_tag="LND56_002614"
     CDS             164..313
                     /locus_tag="LND56_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744480.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            394..990
                     /locus_tag="LND56_002615"
     CDS             394..990
                     /locus_tag="LND56_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK6744481.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1003..1902
                     /locus_tag="LND56_002616"
     CDS             1003..1902
                     /locus_tag="LND56_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK6744482.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1905..2036
                     /gene="ssb"
                     /locus_tag="LND56_002617"
     CDS             1905..2036
                     /gene="ssb"
                     /locus_tag="LND56_002617"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6744483.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2058..2390
                     /gene="ssb"
                     /locus_tag="LND56_002618"
     CDS             2058..2390
                     /gene="ssb"
                     /locus_tag="LND56_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6744484.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2412..2669
                     /locus_tag="LND56_002619"
     CDS             2412..2669
                     /locus_tag="LND56_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744485.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2828..2950)
                     /locus_tag="LND56_002620"
     CDS             complement(2828..2950)
                     /locus_tag="LND56_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBK6744486.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3140..3364
                     /locus_tag="LND56_002621"
     CDS             3140..3364
                     /locus_tag="LND56_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744487.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            3812..>3984
                     /locus_tag="LND56_002622"
     CDS             3812..>3984
                     /locus_tag="LND56_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK6744488.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGD"
BASE COUNT         1416 a          695 c          804 g         1069 t
ORIGIN      
        1 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
       61 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
      121 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
      181 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
      241 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
      301 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
      361 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
      421 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
      481 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
      541 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
      601 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
      661 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
      721 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
      781 cgctatggga atcctattat agcttatgat ccagaaagaa ctttatgtga catttggaat
      841 ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta tatggagtcg
      901 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttaccaac agacaaaacg
      961 atgcgttctt atattatggc tttaaattaa aggagaagat acatgacacc tacgcagtta
     1021 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     1081 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     1141 gtgttaaaag gcggcttctt gattggctca aagtatggca ttgaaaatcg cacaaccaaa
     1201 gatattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattaac aacggttcta
     1261 aacgaaattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca aggaatgaaa
     1321 gaaacgagag aagccgatta ctatccagga tttagcttac gtgtgctggc acatttggaa
     1381 aacatgcggc cagattttaa ggtagatgtg actacaggtg actcgatcta tcctgcaacg
     1441 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     1501 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     1561 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     1621 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     1681 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     1741 aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga gacaatggat
     1801 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct
     1861 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     1921 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     1981 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag
     2041 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     2101 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     2161 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaagta
     2221 aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccgcca
     2281 cctttgaaca acggaataca gcgacgccta acaacaatgg attgaacgct tcacagaatc
     2341 catttggtgg acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag
     2401 aaggagtaag catggcaaaa aaaattagtc tgacgatgga tctattgata cccgtaattg
     2461 catttataga cgggatttct ttgggcgtca ctgaaaatcc tttggtaaat agaattgcga
     2521 aaggatcact atttcttgta ttcgtgtatc ttgtctgttc ttatctttta aaaagagcag
     2581 gaaaggataa ttccttttgg cgattgacta aagtcacagt agcagcgaca gctgttatac
     2641 ttacggctat tctaccgctt tatatgtaga gttaacggga aatcgttgaa cttcttgcga
     2701 aatattgcta taatgaacat gaaaaagagg agttgcgcta acaacccctc attaacagca
     2761 ccgattaaag aacggtggca gaatattagt tatttggaaa taaccgctga cctgctaaag
     2821 tagacggtta tttctttttg tcatttttaa gcatatttac aatcaaacta atcaaagcaa
     2881 tggtaaacat accaaaacca agaatggttt gaattgtctc atatgcggac aaaaggctta
     2941 ctcctttcat agattttagc acttacattc ataagcacca ccacctttcc agaagtcgcc
     3001 accgtcttca aactttactg tcttgactat tatagcatat ctaaaatcga ataaaaattc
     3061 tcaattttag ctgtttttgc tataatttta gcgaggtgat cgtgatggaa caatatattg
     3121 gtttactcga taaattaggg tgatcaagac acagccattg cttgtgcgat ttacattacg
     3181 cacgagtcat gaaccaatca attgtgtagt ggccaatata gaaatagcgg ataaattgct
     3241 gattttggaa gaaggcaagt atactatttc agtgacaggt cacttcaata aacgaaatca
     3301 actagtcatt gaatcaatgc aaattagaaa ccctgatcat tttacgagat taatgggcat
     3361 ataaataaaa gaagagctgc agtcctaaat gaggattgca gctctttttg ttttacatag
     3421 aaacaagaaa aatgaaaagg cctttttagg aaggcgcaag cgccttctca attgaaaaac
     3481 ctctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gttgcacgtt
     3541 cactaagata aggggtcttg caaagcaaga caaaatagaa aaaacttcct ctcaccaaaa
     3601 ggttcgttcc tgttcgctat ctctttattg atttgccaga ccggcaacaa gttgcctttt
     3661 atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcacgccctt gtccttgtcg
     3721 cttttagttg attcattctg gccaacaaat caataaagaa agaactaacg aaaggaagta
     3781 aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt attggggtgt
     3841 tcccttcagc gaattattcc caaactatca tgcaccatat actgtcgaat gcgattgtgg
     3901 agaaagagcg aaatgtatca aaagctattg tttgtatcaa tgtccaacat gcgggaaaaa
     3961 atataccttg tcttatggcg atta
//
LOCUS       DAFCLG010000168         3947 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFCLG010000168 DAFCLG010000000
VERSION     DAFCLG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.171"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..418
                     /locus_tag="LND56_002623"
     CDS             <1..418
                     /locus_tag="LND56_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744489.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="LND56_002624"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="LND56_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6744490.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="LND56_002625"
     CDS             1305..2459
                     /locus_tag="LND56_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6744491.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVQITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(2556..3848)
                     /locus_tag="LND56_002626"
     CDS             complement(2556..3848)
                     /locus_tag="LND56_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6744492.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT         1282 a          705 c          824 g         1136 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc aaatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat attttggctc tttgtcaaat gatgttggta aagagaaatc ttagaataat
     2521 tggagatcaa gtggatcagt tcctcttgat cttttttagt tgaaaatcaa tgcgttgcca
     2581 tgacgaatca tgatgcgcaa tttgaaatta taaaagctac gaaagccata tgcatttcgt
     2641 tttaacactt tgatatggtt gttcagacat tcaagtgggc catttgaata actttgttgt
     2701 aaagcatttt tgatttgtgg cagaaatttc tggaacgttc tcaaggtcgt ctgatagcct
     2761 tcgggaagca tgatattatt ggtaagtagt tcttcaaacg atacgtagtc tcgttccttt
     2821 acagcgtaaa gaaagtcttg atataactga tagcctacac gcaaagcagg gctccctttc
     2881 agtaagcgat ccacaatatc ggtctctgtt aaatgagctt tgaagccagg tcgccaaagg
     2941 cgtcgagtat aatcaagctt gcgttcatct ttttgaagaa gtttccagta tctttttaat
     3001 tgcttaccta attgatagtg tttttgttct ttgcttttta atagttggtt cgtttctttg
     3061 attcgatgat tgcgaaaggt acgaccaata tgttggacga tgtgaaaccg gtcaataatc
     3121 agctgtgctt ttggaaacag ctttttaacg agcgatacat aaggagcgta catatcaatc
     3181 acgatccatt cgaccgcttc acgaatttct cgtgaaaaat gactaaaata acgttttaaa
     3241 aaaggcagtt gtcggttctc gacaatgtcc aataagcgct gtgtttgtcc atccatcatg
     3301 atgaaactca tagcacctga aacaccccga actgatttga attcatcaaa acaaagtacc
     3361 ttaggtaacg gtgtccgaaa aggattcaac ggacgataaa atcgcttcat aatacggtaa
     3421 acagaagaga ccgatagatt tttctgagcg gcgatctctg tcattgaggt attttttttc
     3481 agacgagtca cgacagacca gcggactttc tcactgatga aacaatgttt tttcgcaaca
     3541 gaagtatctg cgacaaaggt ccgctgacaa gctttgcatt tgaagcgttg ctttttcaat
     3601 tcgaggtagg tacggtattc agaaacatcg ttcattaaca accggacggt tgttgtaccc
     3661 caacgcacaa tatctgaagc aatctggtgt ttacagtgga tgcattcttg ggctgtataa
     3721 atcaagtttc cacgataaat atgacagatt tgccctttgt atttttcttt tgtgagacaa
     3781 ttttcgttaa aagtaactga taagtctaga atatctaatg tttctttgat aaggtgagtg
     3841 taagacatct tgattcctcc ttttgggttg gttcgccaaa ttaattctaa aggatttcaa
     3901 ggtgtctttt ctatatgttt tggaaaataa aaaagatgct ggtgtca
//
LOCUS       DAFCLG010000169         3816 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFCLG010000169 DAFCLG010000000
VERSION     DAFCLG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.172"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..311
                     /locus_tag="LND56_002627"
     CDS             <1..311
                     /locus_tag="LND56_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744493.1"
                     /translation="GKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVING
                     IFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL
                     "
     gene            complement(437..1114)
                     /gene="nth"
                     /locus_tag="LND56_002628"
     CDS             complement(437..1114)
                     /gene="nth"
                     /locus_tag="LND56_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK6744494.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1121..1819)
                     /locus_tag="LND56_002629"
     CDS             complement(1121..1819)
                     /locus_tag="LND56_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6744495.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2087..3271
                     /locus_tag="LND56_002630"
     CDS             2087..3271
                     /locus_tag="LND56_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBK6744496.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3277..3720)
                     /locus_tag="LND56_002631"
     CDS             complement(3277..3720)
                     /locus_tag="LND56_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6744497.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          991 a          754 c          634 g         1437 t
ORIGIN      
        1 aaggaaaatt tatggaaaga agctctcatg caatgattct cactttttta tggttgttgt
       61 tcaccagaat ctatattttt ctgatgagcc tgaaaccgcc caaatccatt agtttatgtt
      121 tagttataaa tggtattttt ttattatcac ttatcatttt tattacacct ttagatagat
      181 acgtcactta tttatataat cctttggaat actggactta ttttataggt atattggaag
      241 gcattatttt ctacatcggg tattttccca ataaaaataa caacttttat ttttatcgca
      301 ataaattgta ataagttacc atcctagcta ctgagtatgt acatttactt tttcatgcaa
      361 aaaagataaa cgaaacagct tttctccata ataacaagat aattcataca tttatttgca
      421 attaaagaaa ccacgtctac aggcgtggtt tcttttttag cgccttttct tttagacgca
      481 tccgattttt cccatcttga catattgata gcaaaggaca tacctcgcac ttgggatttc
      541 ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg atgtgtcttt acccataatt
      601 cttgtggtac tttccgcatc agtgtttctt ctacttccat gaccgtagca tctagtttgc
      661 agatcctcag gcgttttgag acacgttcca cgtgggtatc tactgcgatt gctggtatcc
      721 cgaatgcatc ccccaatacg acatttgctg tctttctgcc tacaccagga agagacatca
      781 attcttctct tgacgttggc acttgcccat cgaaacgttc aatcagctgc tgtgcacacg
      841 ctttgatgtt ttttgcttta tttcgataca aaccaatcgt ttttatttta agaatgattt
      901 catcgattga agcttctgct aacgcatcgg gtgtcggaaa agaggcaaac aaatctggcg
      961 ttgctttatt cactgataca tctgtcgcct gggcacttaa gatcactgca atcaataact
     1021 caaatggatt attgtgcttg agttctccat gtgcttcggg aaacattcca tacatcgttt
     1081 ctagagcttc cattgttttt tgtttgctta acatattccg ctactcctcc aaccaatttt
     1141 tcagtgagac tttcggcaat tcttcttgtt gagctgactg ttctacttct ctttgaagaa
     1201 gctgctgttt tcttcgtttt tggtcttctt ccacttgttg ttttgtacgt agattttttc
     1261 tttcccacga tagcaaaatg cggtcaacgt aattaaaact ataagcctga tttaatacgg
     1321 cttcccgcag tgctagcttc aaaatttccg gctgataatg gtcttcttct agccattgtc
     1381 cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa ttcttgttca aataattgat
     1441 acactgactg gatttctttt tcttgtgttt tttcttcgta ttttttttct tgtgttttta
     1501 aatactcacc taatagatca tagatggggt aaagattata tcgatctgct tttttaccgt
     1561 tgacaatcgt cgtttctatc ttgataaatc catttgttac gagacgatcc agtatttgaa
     1621 aaatctgatc ttgtttgatt cccatatcca tagcaatcat ctgcaaatca gggaaagtat
     1681 ctccttcaag ctgtgccata tgcaactgta gaacaaataa aaattcttca gattgtagac
     1741 cgatcctatg ataattttta agtaaaagat tagatatgac tgtttgtcca gcgcttaagt
     1801 aattttctaa attcaacatg atttctcctc cgctcaatca cagtatacgt aaaaaaaaca
     1861 tgggaagcaa gaacaggttt cttttctatt tacttcactg atcggtcaga tttacaatgt
     1921 cgtctacttg atttttgttt gttcatgttt ttgatgcgta tcttcacttt tcggaataaa
     1981 taaattaagt agatttcgct gttccttaga atgttgacaa ggaaacaaaa ttactataca
     2041 ctatttttgt ttccaagtta acattattgc ataaaggagt ttttacatgt acagtttcac
     2101 aaataaacat tctaaagttt ttggatacgt ctctgtctta ttcactggat taggatttac
     2161 tatcgtcagt cctgtacttc cgtttttaac gctgccttat tctcactcca tacatcagca
     2221 agctttctat atcactttgc tcatgtctgt ttatgctcta gctgccttct tatctgcccc
     2281 cattctcggt agcttaagtg atcattttgg gcgtcatccg atccttatta tcagtttatt
     2341 aggatcaagt atcggatatt tgctttttgg tttaggaaat tcgatatgga tgttgttcct
     2401 tggtcgtatc attgaaggat tgacggcagg agaaatctcc accttatatg cttactttgc
     2461 cgacatcacg gaacctaatg aaagaaccaa agtttttgga tggatgggtg ctttagtcgg
     2521 tataggaaca acgctgggtc ctattatagg gggtctatta gctgagttgg ggaattctgt
     2581 tcctatattt attggagcac tctttacttt cttaaatgcc gtttatggct acacatttat
     2641 gctagaaagc cttccaataa aaaaacgttc agtagattta agtttctccc atgtaagacc
     2701 ttttcaccaa ttacaacaat tattcaagat ttcatccgtt attcctttat tgacagcagg
     2761 ttttgctgtt tggttggcag ctggctctct tcaatctatc ttttctcaat tttcgattga
     2821 cgcctttcag tggaaggctg ggttggttgg tttaagcttc tctttgattg gaatcttgga
     2881 tactgtctcg caattgttca ttatgcctcg attgctcaaa aaattttcag aacagcagat
     2941 cacaagaata gggatgttca gtgaaatcct cgcctatcta ttcatcacat tatctggaat
     3001 tcttctactg ccgattttgt ttttatttgg gattatctgt tatggtttcg gtgattctat
     3061 ttttactcct gtattcaatg gccaactatc caattctgtc tcagataacc agcaaggtct
     3121 tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc gtcatcggcc cgctaatcgc
     3181 aggacagctt tatgctgcag caccatttct tccgacggta ttcggatttt tgcttatgat
     3241 ttttgcctat tatcactaca ataaaaaata gtagcttcat ttgctacttc gtataaattc
     3301 tctgtatcga ccgataaaac gaaatactgt gtcaaattct tcttttgaca tattttcaaa
     3361 tacagtagca tctcgctgta cccatttttg atggagttgc tcgtgtctat tccttatttt
     3421 ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta ttctcttcgc tttgataagt
     3481 gtttatgatc cctctagcta tcagtttttt tgttagctta ctgatggctc ctctagtcat
     3541 gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg attttcccga tatgttcaat
     3601 cacttctagt tcatttagag taaatcctgc aaattctttt tccatttctg gtttattacg
     3661 atgaataatc tcattagcta aatccagata ttcttctatt agcagttgtc ttttatccat
     3721 ttctacacct tctttttctt tcattatatt gtttttttgt ttcctattca acaaagataa
     3781 aaatattttg taggaaaacc caatctgaaa ccaaga
//
LOCUS       DAFCLG010000170         3785 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFCLG010000170 DAFCLG010000000
VERSION     DAFCLG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.175"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..171
                     /locus_tag="LND56_002632"
     CDS             <1..171
                     /locus_tag="LND56_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6744498.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="LND56_002633"
     CDS             406..642
                     /locus_tag="LND56_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744499.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="LND56_002634"
     CDS             649..2601
                     /locus_tag="LND56_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6744500.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="LND56_002635"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="LND56_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6744501.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="LND56_002636"
     CDS             3400..>3785
                     /locus_tag="LND56_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6744502.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAFCLG010000171         3717 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFCLG010000171 DAFCLG010000000
VERSION     DAFCLG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.177"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..243
                     /locus_tag="LND56_002637"
     CDS             <1..243
                     /locus_tag="LND56_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBK6744503.1"
                     /translation="CTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQK
                     GSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            259..408
                     /locus_tag="LND56_002638"
     CDS             259..408
                     /locus_tag="LND56_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744504.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            482..1075
                     /locus_tag="LND56_002639"
     CDS             482..1075
                     /locus_tag="LND56_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK6744505.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1091..1990
                     /locus_tag="LND56_002640"
     CDS             1091..1990
                     /locus_tag="LND56_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK6744506.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1993..2478
                     /gene="ssb"
                     /locus_tag="LND56_002641"
     CDS             1993..2478
                     /gene="ssb"
                     /locus_tag="LND56_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6744507.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2844..3104
                     /locus_tag="LND56_002642"
     CDS             2844..3104
                     /locus_tag="LND56_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744508.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3545..>3717
                     /locus_tag="LND56_002643"
     CDS             3545..>3717
                     /locus_tag="LND56_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK6744509.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGD"
BASE COUNT         1348 a          637 c          724 g         1008 t
ORIGIN      
        1 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
       61 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
      121 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
      181 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
      241 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
      301 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
      361 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
      421 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
      481 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
      541 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
      601 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
      661 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
      721 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
      781 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
      841 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
      901 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
      961 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     1021 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     1081 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     1141 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     1201 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     1261 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     1321 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     1381 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     1441 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     1501 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     1561 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     1621 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     1681 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     1741 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     1801 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     1861 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     1921 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     1981 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     2041 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     2101 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     2161 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     2221 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     2281 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     2341 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     2401 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     2461 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     2521 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     2581 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     2641 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     2701 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     2761 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     2821 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     2881 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     2941 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     3001 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     3061 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
     3121 agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     3181 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     3241 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     3301 agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     3361 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     3421 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgatttatt ctggccaaca
     3481 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     3541 aaacatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     3601 tcatgcacca catactgttg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta
     3661 tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgatta
//
LOCUS       DAFCLG010000172         3531 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFCLG010000172 DAFCLG010000000
VERSION     DAFCLG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.178"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="LND56_002644"
     CDS             complement(<1..182)
                     /locus_tag="LND56_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6744510.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="LND56_002645"
     CDS             517..951
                     /locus_tag="LND56_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6744511.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="LND56_002646"
     CDS             974..1294
                     /locus_tag="LND56_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744512.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="LND56_002647"
     CDS             1435..1626
                     /locus_tag="LND56_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744513.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="LND56_002648"
     CDS             complement(1697..1954)
                     /locus_tag="LND56_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBK6744514.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="LND56_002649"
     CDS             2112..2375
                     /locus_tag="LND56_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744515.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="LND56_002650"
     CDS             2399..2587
                     /locus_tag="LND56_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744516.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="LND56_002651"
     CDS             complement(2670..2855)
                     /locus_tag="LND56_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744517.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="LND56_002652"
     CDS             3070..3441
                     /locus_tag="LND56_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6744518.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1326 a          501 c          702 g         1002 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa t
//
LOCUS       DAFCLG010000173         3522 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFCLG010000173 DAFCLG010000000
VERSION     DAFCLG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.179"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            130..951
                     /gene="racE"
                     /locus_tag="LND56_002653"
     CDS             130..951
                     /gene="racE"
                     /locus_tag="LND56_002653"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK6744519.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            954..2309
                     /gene="rph"
                     /locus_tag="LND56_002654"
     CDS             954..2309
                     /gene="rph"
                     /locus_tag="LND56_002654"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK6744520.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            2310..2828
                     /locus_tag="LND56_002655"
     CDS             2310..2828
                     /locus_tag="LND56_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6744521.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            2935..3435
                     /locus_tag="LND56_002656"
     CDS             2935..3435
                     /locus_tag="LND56_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6744522.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         1200 a          595 c          800 g          927 t
ORIGIN      
        1 aagtcacaac cgcttttcct ctttccattc gaaaaaaaga gatttcgatt gttttaagca
       61 agtaagagca cttgaaaatt cacgctaaaa agtacacaat agggaggaaa gaatttattg
      121 aaaaaaagga tgatacgatt gacagataat cgccctatcg gatttattga ttcaggtgtc
      181 ggcggcttga ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa tattttattt
      241 gtaggagaca cagcacgctg cccatatggc cctagacccg cggaacaggt aatccagtat
      301 acttgggaaa tgacggatta tctggtggag caaggaatca agatgctggt gatcgcctgc
      361 aataccgcaa ctgcggtggc tttagaagaa atcaaagctg ctctttctat tccagtcatc
      421 ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa acaagttggc
      481 attatcggta cgattggtac ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa
      541 gtaccagaat tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt
      601 aatgaatacc attcatcggt ggcgaaaaaa attgtggcag aaacattagc tcctttaacc
      661 actaaaaaaa tcgatacatt gattttggga tgcacccatt atccattatt acgccccatc
      721 attcaaaatg taatgggaga aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt
      781 gaagtatcta tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca
      841 ttatgccagt tttatacaac tggctctgcc aaacttttcg aggaaatagc tgaagactgg
      901 cttggaatcg gacacttaaa tgtagaacat atcgaattgg gaggaaaata aggatgcgtc
      961 atgacgggag aaaagcccaa gagcttcgca aactggagct tcaaacaaat gttttcaaat
     1021 atccagaagg atcggtagtg atccgttttg gtgatacaac agttatttgt tctgcaacaa
     1081 tagaaaattc tgttccccct tttttgagag aaacaggaac aggatgggtg acagcagaat
     1141 atagtatgct gcctcgagca acgagtacga gaaatcgtag agaaagcagc aaagggaaac
     1201 tttctggtag aacaatggaa atccagcgtc ttattggtcg ttccttgcgt gcagtagtag
     1261 acttggaaaa attaggagaa cgtagtatca tcgtagactg cgatgtgatc caagcggatg
     1321 gcggtactag aacagcaagt atcactgggg catttgtggc attgaaatta gccattgaaa
     1381 aacttttaag agaaaaagaa ttgtcagaag atccaataaa agaacatctt gctgcagtta
     1441 gcgtaggaat cttaccagat gggacttgcg tcacagattt ggattaccaa gaagattccg
     1501 cagcacttgt agacatgaat cttgtaatga cagaatctgg aaaattcgtg gagattcaag
     1561 gaactggaga agaagcaacg tttgatggtg aacagttgaa tgaaatgctc ttttttggaa
     1621 aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc tttgctaaca gagtttgctc
     1681 aaaatgatga gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa
     1741 aagcagaaga gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa acattacttg
     1801 actatcctga attgccagat gtggaagaaa ctggcagcac atttgaagaa aatgcccgat
     1861 taaaagcaga aacgattgca caattgcttg atcagccagt cttagcagat gattcaggtt
     1921 tgaaagtgga tgctttaggc ggtatgccag gaatctattc tgcacgtttt gcaggggaac
     1981 aaaaaagtga tgcggggaat aacgccaaat tattatatga attgacagat gtgccggatg
     2041 aaaaaagaac agcacaattc cattgtacgt tagtattcgc ggcacccaaa aaagacagcc
     2101 ttgtagtaga agcggaatgg cccggtcgag tagcccgtat accatctggc gaaaatggct
     2161 ttgggtatga tcctttattt atcccagaag gtaagaaaca aacagcagca gaattatcga
     2221 gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt
     2281 ggaaacaatg gctggaagga gagagataga tgcggtattt agtagtaagt gataatcatg
     2341 gagacagaga tatcctcgtt gacttagtag atcgttatcg ttctgaagtg gatatctttt
     2401 tccattgtgg tgattcggaa ttagaagctt ctgacccatt atgggataca ttccaagtgg
     2461 ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc caaactggtc
     2521 aggatacagt attcatgact cacggtcatt tatcgaatgt ccgatttggt ttgacacagt
     2581 tagcaatcga agctcgagct gcaactgccg atatggtgtt ttttggtcat acccatcaaa
     2641 taggctgtga gatggatcag aaaatcctgt ttttgaatcc tggtagcatt tctcagccta
     2701 gaggcccgat ccaaatccca tcatatgctc ttatcgaaag tttagatgac caaatttctg
     2761 ttcaatacta taaccgtgcc catcaggcag ttgaagagtt ggccttttct ttttcgagag
     2821 agcgctgaat aaaaaaactc taatttttaa taaaaataag ctttatttta tgaattttgc
     2881 atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca tcagatgatt
     2941 ggacctatag taagagaaat gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa
     3001 aatgtggcaa atgttatgta tcatcatcca ttgtcccatg gtttacttgt gttatcgaag
     3061 gtcggttatt ccaaaattcc tgtattagga aaagatgatc actttgtagg actagtaagt
     3121 ttatcagatg tggtcaataa aatgattgac ttgcaaacaa tcagcatgga cccgttagaa
     3181 ggactaacgg ttgctgatgt gacagaaaca gatgttccga caattgatga aaattgggaa
     3241 ctagaagaag tactccattt gttagtcgat tcgccttttt taccggttgt cacagaagat
     3301 cggattttca aaggaatcat tacaagaaaa gagctgctta aagccgtaaa cttcatggtg
     3361 catgaattag aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa agaacaaatc
     3421 gatatcgtga gctaaagaac gagaaactaa aagataatca aaaaataagt agaaaaaaca
     3481 gcgtaccagt tttgacttta tcttgaaaaa gaggctggga ca
//
LOCUS       DAFCLG010000174         3459 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFCLG010000174 DAFCLG010000000
VERSION     DAFCLG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.180"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..714)
                     /locus_tag="LND56_002657"
     CDS             complement(229..714)
                     /locus_tag="LND56_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBK6744523.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    804..1149
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1194..1835
                     /locus_tag="LND56_002658"
     CDS             1194..1835
                     /locus_tag="LND56_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6744524.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1854..2489
                     /locus_tag="LND56_002659"
     CDS             1854..2489
                     /locus_tag="LND56_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6744525.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2507..3322
                     /locus_tag="LND56_002660"
     CDS             2507..3322
                     /locus_tag="LND56_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6744526.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1127 a          569 c          739 g         1024 t
ORIGIN      
        1 aataaaattc cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt
       61 agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt
      121 tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa
      181 ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc
      241 ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat
      301 caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc
      361 atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt
      421 acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt
      481 ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac
      541 aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg
      601 tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga
      661 gatacccagt ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca
      721 tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat
      781 gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt
      841 tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag
      901 tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg
      961 ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac
     1021 gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac
     1081 gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac
     1141 gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt
     1201 atttgattga agttatgccg caattattag taggagcact cacaacattg aaattatttt
     1261 tatttacctt gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc
     1321 attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc
     1381 tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc
     1441 gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct
     1501 ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat
     1561 taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac
     1621 cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag
     1681 gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc
     1741 ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca atcgtaatga
     1801 aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg
     1861 aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa
     1921 tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc
     1981 ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg
     2041 taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag
     2101 acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg
     2161 tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag
     2221 ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg
     2281 tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa
     2341 gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag
     2401 atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc
     2461 aattactaaa agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag
     2521 ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac
     2581 ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga
     2641 tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga
     2701 tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg
     2761 gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc
     2821 caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca
     2881 agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag
     2941 attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt
     3001 aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat
     3061 cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta
     3121 tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga
     3181 ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc
     3241 cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga
     3301 agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc
     3361 tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg
     3421 ctcacaacct cttggtaaac ggtgttcaaa agccagttt
//
LOCUS       DAFCLG010000175         3400 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFCLG010000175 DAFCLG010000000
VERSION     DAFCLG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.181"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..276
                     /locus_tag="LND56_002661"
     CDS             <1..276
                     /locus_tag="LND56_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBK6744527.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="LND56_002662"
     CDS             419..640
                     /locus_tag="LND56_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744528.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="LND56_002663"
     CDS             656..1498
                     /locus_tag="LND56_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744529.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="LND56_002664"
     CDS             1561..3363
                     /locus_tag="LND56_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBK6744530.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1230 a          469 c          703 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga
//
LOCUS       DAFCLG010000176         3355 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFCLG010000176 DAFCLG010000000
VERSION     DAFCLG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.182"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..1148
                     /locus_tag="LND56_002665"
     CDS             108..1148
                     /locus_tag="LND56_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6744531.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1972..2304
                     /locus_tag="LND56_002666"
     CDS             1972..2304
                     /locus_tag="LND56_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK6744532.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2923..3264
                     /locus_tag="LND56_002667"
     CDS             2923..3264
                     /locus_tag="LND56_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK6744533.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1250 a          457 c          639 g         1009 t
ORIGIN      
        1 ttaaaaaaca gttttaatgc gtttgcattt tttatttatc tttgctattt tcgaaaagag
       61 taggtaattt tacataaata aaacaacgtg aaagtagagg atctacaatg actgatttta
      121 aattttttaa ggcggataga gtttacaacg aattatttta tcaatttcca aaagtcttta
      181 ttgtttctga cgaatacaaa aaaatgaaag attcaactaa gattgcctat atgcttttaa
      241 aagcaagatt agagatcgca atcagcaaac ggcaaatcga tgaagaaggt aatgtttatt
      301 ttacttatac gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa aaagcgatag
      361 caatcaaaaa agagttggaa tcctttggtt tattattaca aaagcagatg ggatttaaca
      421 aacagttagg gaaaaataat cctaatagac tatatctagc agaattaaaa gtctcagaaa
      481 atgatatcta cttactcgaa aaatttgata gagagaatag ggaaaacgtt gataaatcag
      541 agggtatgaa aatcataccc accctcgacg aaaaatcaga cgctgaatcc cttggggctc
      601 aagagggtat gaaaatcata ccgtgccaaa acgttgataa atcagagggt atgaaaatca
      661 taccagaact taataataat atattagaca ctaatagaca caatatagac actgaaaaag
      721 accgcctaca agatcaattg ttgttagaca attttgagac aattatgaca aacgacagca
      781 ttgctacgtt tgtccctgaa cgagtattaa atttgataaa aacattttct tcaagttaca
      841 gtgaagctca aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct gaaatagaaa
      901 gtgatatttc gatagttttt gaagaactcg atagttatta tgtcaatgca gaacaagaat
      961 tatacacgac actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc gaaaatatcc
     1021 agaacctgat ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata gctgctaaag
     1081 tatcaagtga aaaacgtttg aattatgcaa gctccccaag cactattacg aaagactggt
     1141 tagagtgaaa ctttactgac aaagtgattg ttgcttcatg gagtaacatt aaaattaaaa
     1201 attttttatt attctaatta tattacgaat attacaaaat aatagctatt ttaatagaat
     1261 ttataagtta aaatattatg ataaaaatca caaacgtagc cttttacgtc ataagtgtta
     1321 aaagaaataa ttaatttcaa atatagaaaa gggaacgtat gtttgcatac tccttttttt
     1381 tgagttataa taatacgcga agagagataa tttcattatt taaacaagct aaaaatcttg
     1441 atattacttc ttttcaggca atacgaatat tattcgctta cattttttgt ataaatttaa
     1501 ttgtctgcta tgcttgtgat gaattgattt tcgttttgca cttttggatg ataaatttgg
     1561 acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc ggaaggctgg taaccttcgt
     1621 cagcgttcat acacggcatt aagatttggc ccttaatgcg ttaacctttc agtgttacgg
     1681 tattacccgc aatggattta gtataacaga tattcaaaag tgataccact ttttttggct
     1741 gtgttaaggt taaaaatacg tgatattacg atgaatctat gcctaagcat aatgttataa
     1801 aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa attagaggga acgcatgttt
     1861 ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc aattctctat ttttgaaaaa
     1921 taacaagaaa aacttgttaa aaatatttaa agaaattggg tggtttattt tatgtcatat
     1981 acaggaattt tgagtctcaa agatatttgt cactacggta aacggtgtac cgctacagag
     2041 aaaatcacga agaaactatc aactggccaa agtaaaaccg ttgtgcagtg taagaaatat
     2101 ataatccaga aagacaaagt ctcagaggaa atgatttatt acattgggaa acaaaagcaa
     2161 ataattttaa aagatcctat ccccttaaaa gagctatatc caacaatcaa acatgtttat
     2221 gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg taccgcaaaa
     2281 gggatggggc gtttgatcgg ctgaattgat cattctcata acttgtattt tatcgttttt
     2341 cgcttgcttg aaaatattgt cgagacttct aaaggaactt agagtcacta gaaaaataag
     2401 gagtagactt gatctatcaa caattccctt cggggagtga cggggtaggt tttaacgatt
     2461 ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg gtgaaatgaa
     2521 tttgtgtaaa taataagcgc agagtaaaca agagggaatt tagaagttag acaagttaga
     2581 atagcgtcta gattaatcat gagcagctac taagggatct agactttgac gcaaactcat
     2641 ggaataagga gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt gtcgtgtgct
     2701 gcagtacatg tagatacttg cgaggacgtt gattattgga tcaatcgagc atttaccgct
     2761 gtctactagg gtttctcgtt tcggacgcca taaacgagtc gaaatttttt atgtattttt
     2821 aagtaaacga aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa aattaatata
     2881 aagcatagag aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg
     2941 aatatacgat tgggcacaca tactattaag gtttctttcc aacgttggga ttacaaagga
     3001 tttgtgacgt ttagaagggg cggaaattgt aaaggattag atgttttagc attagacgag
     3061 gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt gctacctgaa
     3121 gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta
     3181 tctgttgaag atacttggtc atatttgagt gattatattg tcagctttga aatcattgaa
     3241 tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttttacagat aaaggcttag
     3301 aaggagtcac gacgattaat aaaaaggtag tattacctta ggcattgagt aagga
//
LOCUS       DAFCLG010000177         3335 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFCLG010000177 DAFCLG010000000
VERSION     DAFCLG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.183"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..1166)
                     /locus_tag="LND56_002668"
     CDS             complement(552..1166)
                     /locus_tag="LND56_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6744534.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1772..2149)
                     /locus_tag="LND56_002669"
     CDS             complement(1772..2149)
                     /locus_tag="LND56_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744535.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2449..3330)
                     /gene="galU"
                     /locus_tag="LND56_002670"
     CDS             complement(2449..3330)
                     /gene="galU"
                     /locus_tag="LND56_002670"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6744536.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1023 a          676 c          507 g         1129 t
ORIGIN      
        1 agattagaga aaatctcgtc ctgttttttt cattttacaa tcattgaaaa attgaatttc
       61 attgcaaaaa aaataataaa cacttataaa tatccattat ctatccttca atttttgtgt
      121 attttatttc tattagaaaa aggtatcctg ttggacaatt ttgttatatc aggtaaactt
      181 cattgcaata atgataacaa atgtactata attactttgt aaagagtttt ggggaagact
      241 ctttcatcaa cgtgtattaa gagacaagaa agaatccata tggattcttt cttgtctctt
      301 tttccactac tttttatttt tctgacacta catattctta taactcattt ctcataactg
      361 tgaaacaact tataaaaata acaaacttta aacaaaacaa gcttaaaatc aaagatgatc
      421 catcttaaaa taactaaaac aaaaaaagta gtcgttgtta atcctcatta ataatcgttt
      481 tctttttttc aaacgctcca tttttatgtg gaacattatt tttttaagga actttgtcaa
      541 catgtggata actattttcc acttgtgtcc tggactagct gttttgctgt aaaatacaaa
      601 acttgctgca gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc tggatctaga
      661 tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac caaatcagca
      721 aattcaaata atcgcaaacc actgctatgt tgcgatttga tacttcgtga caaatcaaat
      781 ccagtaacaa tgattaatgg atgaccattt tctttgatcc actgagctag ttcgatgatt
      841 gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt cttctgattt gacagaaagc
      901 tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat agacacctcc ggctggattt
      961 tcaatcaata tacttggtac aagatccggg gtttgatccc aaaattcaaa tgctacacca
     1021 cttagtgtgc ggctagcata tatctgcaat ctaccacctt gattaatttg tttcgcgatc
     1081 caagtgccag cttgaagcat cagcggctct tctatcctta atttctcttc caattggtga
     1141 taatagtcaa aataacataa actcatttat ctctcccgcc tagctatact tgacatgaat
     1201 agaaacagct gcctaatcta aggaagtaca taaaaaaaca gtagttagac agtaacacat
     1261 gtggtctttg ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca ataccttgac
     1321 atagcaaata aaacaacgtg cagaccatgg aaaatatcta cataggggta gaattttcac
     1381 cctagattag gtacaataaa ttaggtcgat gcactgcatt gactatggca aaatgatcgg
     1441 tcagggtgta tgtgtgtgac tggtcattcc ctgattctta gcgccaacta agggtcaggg
     1501 agcctttttt tattcagctg tttctatctg aatttattgt aagtaatctc ttatccaagt
     1561 gcaagcaaga aaatacaaaa aaacattggt atgatttata tatatataaa taacataaaa
     1621 aaaggaatgg cttatcccga tacaacaagc attcctttaa aaaagattct tttatataag
     1681 tgaaaaaagc ttttttccac caattaaata aatctctcat ctcaatagtt tatccattat
     1741 tttatctttt tttgcgtata cctactaaat attaccgtct tcttctcgtt cttctacttt
     1801 gttcgagaga atctgtacta gaaaaagtag ttgtatgtct accttttctg cctatccgag
     1861 actttctttt catatatggg atataaatga ttagatacag tacagcaaaa aataaagcaa
     1921 atacgcaagc ttcccaacta cctaacgcca attcaaccat caaggtagca accccaacgg
     1981 atgctgcata ccagataaaa atcagaaacg tttctttttt tctgaccatg cgaaacatga
     2041 taaatagact aataagcaat agtggcaaca caaatatcca attgcttcct tccatcagcc
     2101 acttgagtat atcaagcagc gactgtgcaa cagcgactaa ttttcccata gtgaacacac
     2161 accttttttt ataattaaaa aattcgcttt tttattctac tatgaatctt ctggcttggc
     2221 tttcatttct cttatgtaat cattttcttt tatacaactt aaaaaaaaat taaaccatta
     2281 gcataatatt ttctgaatct attcacgaaa gtctttttct atctttcgtt ttccacgact
     2341 caactgatct agagtcaaaa aactaaaacg acagttgcgc aaatgacgca caagctgccg
     2401 ctttaataac aaatagcctt tcattctcag caggccactt atttccagtt aactttctgt
     2461 tcttagcttt ttactcaact ggataatata tgcacttaat gaagtcccaa tttccgaatg
     2521 ttgaagtcca tattcaatat ttgtcttcat atatcctaac ttatcgccta cgtcgtatct
     2581 ttttccttta aactctctcg catagaccgc ctctgtctgg ttaagagtct gaagtgcatc
     2641 cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt tctaatatct caaatatttt
     2701 aggagtcagt agataacgtc cgataatggc taaattactt ggtgcttttc ccatttcagg
     2761 tttttctaca aaccctttta ctctgcaacg acccttttcg ctatctgttt taggatcaat
     2821 aattccgtat ttagatgttt cattttctgg tacacgcatg acggcaacag cggaagcttg
     2881 tgtttcttca taatccatca tcaattgttg agtcaatggg atatcatctt tcatcaaatc
     2941 atcccccagc atgacgacaa acggttcatt tcctataaat gcttttgctt gaagcacggc
     3001 atcccccagt cctcttggat gagattgacg gatgaagtga agattgactt gagtcgtttc
     3061 ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc aaattgtttt ccaattcgat
     3121 atttgcatca aagtgatctt caatgggtct tttttctttt cccgttacga tcaagatgtc
     3181 ttcgattccc gaatccaatg cttcttccac aataaactgg atcgttggtt tatccacgat
     3241 tggaagcatt tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc
     3301 ggcaggaatg actgcttttt tgaccttcac ttgct
//
LOCUS       DAFCLG010000178         3330 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFCLG010000178 DAFCLG010000000
VERSION     DAFCLG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.184"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..2165)
                     /locus_tag="LND56_002671"
     CDS             complement(552..2165)
                     /locus_tag="LND56_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6744537.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="LND56_002672"
     CDS             complement(2306..3214)
                     /locus_tag="LND56_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744538.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1084 a          565 c          579 g         1102 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact
//
LOCUS       DAFCLG010000179         3218 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFCLG010000179 DAFCLG010000000
VERSION     DAFCLG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.185"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..1438
                     /locus_tag="LND56_002673"
     CDS             50..1438
                     /locus_tag="LND56_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBK6744539.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="LND56_002674"
     CDS             1776..>3218
                     /locus_tag="LND56_002674"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBK6744540.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DAFCLG010000180         3201 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFCLG010000180 DAFCLG010000000
VERSION     DAFCLG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.186"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(253..621)
                     /locus_tag="LND56_002675"
     CDS             complement(253..621)
                     /locus_tag="LND56_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBK6744541.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            771..1253
                     /locus_tag="LND56_002676"
     CDS             771..1253
                     /locus_tag="LND56_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBK6744542.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1290..2300
                     /locus_tag="LND56_002677"
     CDS             1290..2300
                     /locus_tag="LND56_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBK6744543.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2415..3128)
                     /locus_tag="LND56_002678"
     CDS             complement(2415..3128)
                     /locus_tag="LND56_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744544.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1080 a          517 c          621 g          983 t
ORIGIN      
        1 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
       61 ttactaagtg gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
      121 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
      181 taggattgaa caaaacctgt ataagctgat gaatagtttc ctcgccagta aaaggtttat
      241 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
      301 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
      361 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
      421 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
      481 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
      541 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tctgattgat
      601 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
      661 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
      721 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
      781 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
      841 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
      901 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
      961 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
     1021 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
     1081 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
     1141 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
     1201 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
     1261 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
     1321 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
     1381 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
     1441 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
     1501 tgatgcaaga tgaaaatatc caaatgatct ttccgccttg gggaggagaa ttattgattg
     1561 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
     1621 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
     1681 ccaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
     1741 tactgaaaac aagagaagga gaatcgattc tacagcaacc ttctgaaaag tatcaaaaag
     1801 agtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
     1861 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggc tgtattgata
     1921 cgatcagtca tttaatcggt accccttatg gagatgtcca atcttttaaa agaaagtata
     1981 atgatggaaa gccgatcttg tggtttctag aaaattgtga gctatcgtca gttgatttaa
     2041 gaagatcact tgttcagatg aaactggcag gatggtttga tggcagctcg gggatcatgt
     2101 tcggtagaag tgcagcagag gatttgttag attataaaat gaaggatgta tatgaagatc
     2161 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
     2221 aactgacatt gatcaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
     2281 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtttatcaa agaccaacaa
     2341 atctaaatag aaaagaaaat gaaagaagct gacagtattt tgccagcttc tttttgtatg
     2401 atctattaga aattttaatt atccataact atcacattga tcagctgttt cacatcattt
     2461 ggattacctg taggaatagc tactacagat gcattatcaa taatttcttg agttgtttct
     2521 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
     2581 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
     2641 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
     2701 ataggtgttc ctgcaggata atgaataaca tgtagttcga aatcacccga tgtatctcta
     2761 aaagtattac ctctaattgc tagccaaaca cgtgcgtact caatttgtag atcagaatat
     2821 cctgctaacg gatctattga ctgagaagac tgtgaggaag ttgtgtctat tgttgaagaa
     2881 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
     2941 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
     3001 gctgaactgg tttgtttgat tgaacttttg tctgtggtcg cagatggatc actattttct
     3061 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
     3121 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
     3181 tgaatccgta aaaccttgtc t
//
LOCUS       DAFCLG010000181         3200 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFCLG010000181 DAFCLG010000000
VERSION     DAFCLG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.187"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            668..763
                     /locus_tag="LND56_002679"
     CDS             668..763
                     /locus_tag="LND56_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6744545.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1065..1310)
                     /locus_tag="LND56_002680"
     CDS             complement(1065..1310)
                     /locus_tag="LND56_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744546.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1655..1939
                     /locus_tag="LND56_002681"
     CDS             1655..1939
                     /locus_tag="LND56_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744547.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1942..2358
                     /locus_tag="LND56_002682"
     CDS             1942..2358
                     /locus_tag="LND56_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744548.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2495..3172)
                     /locus_tag="LND56_002683"
     CDS             complement(2495..3172)
                     /locus_tag="LND56_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6744549.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1065 a          598 c          499 g         1038 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt
     1261 tgaaaatatt cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta
     1321 gaaataaaat cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa
     1381 ataaattttt gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc
     1441 ccgaatttta aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag
     1501 gctgtgccta tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta
     1561 gcttatgtat agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac
     1621 ataaattata cataattgca aaaggagatt ttttatggac attttagttc ggaatattga
     1681 tccaaagttt gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga
     1741 aaaatggagt cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact
     1801 tacgacttac aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac
     1861 agaaaatact caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct
     1921 tatcggagga atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac
     1981 cctagtcatg ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     2041 tcggaagcca ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc
     2101 agcaatcagg caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc
     2161 gcagaatttg ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa
     2221 gtatatcaag aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga
     2281 ccaaagaatc atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca
     2341 aattttctaa aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag
     2401 ccccatacaa tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac
     2461 ttgcttaatg taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag
     2521 aaatctttgg caaatctgac gttaggcttg aaaaagtaaa ttctaatttg gcgattttat
     2581 tgcccttttt tgcttttatt tttgtgactt taagatcatt gaagtatttt tttagttctc
     2641 gtaatgcagg atttaatact ttttgatcaa tgttacccat ttgatagtat tctgggatat
     2701 ccattaagct cctaaactct tcaatacgaa ctacataata accagtagaa cggtactgca
     2761 taagcagtcg gaatagagtt tttgcatacg tagaacgcaa cgacgtgaaa gcagataatt
     2821 caaatctact aaattcagct gttaaaccat taataatata ttctaggtcc ggattaacac
     2881 tgacttcaac aaactttttt gttttattga tttcaaaacc agtaaacaaa acaaagtagc
     2941 gaatagaatc ctcatcttca gtacggtaag taagattaag catatcttta tagagctttt
     3001 ctaaatcacc gacaaaggct tttgtagcag tcatcttata atcacttaac tcttttaatt
     3061 cctcgaaaga aaatctaact tttttcaaac cacgatcttt catacgagcg cataatgcaa
     3121 aaaataaatc catctgctta gcattgaaat ttttcagcgg tactaaattc aattcatttt
     3181 gatatttgac tgttaattcc
//
LOCUS       DAFCLG010000182         3194 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFCLG010000182 DAFCLG010000000
VERSION     DAFCLG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.188"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..251)
                     /locus_tag="LND56_002684"
     CDS             complement(21..251)
                     /locus_tag="LND56_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744550.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LND56_002685"
     CDS             complement(687..1253)
                     /locus_tag="LND56_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6744551.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LND56_002686"
     CDS             complement(1253..2209)
                     /locus_tag="LND56_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK6744552.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LND56_002687"
     CDS             complement(2209..2865)
                     /locus_tag="LND56_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK6744553.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         1010 a          614 c          559 g         1011 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttat
//
LOCUS       DAFCLG010000183         3107 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFCLG010000183 DAFCLG010000000
VERSION     DAFCLG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.189"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(95..1030)
                     /gene="murB"
                     /locus_tag="LND56_002688"
     CDS             complement(95..1030)
                     /gene="murB"
                     /locus_tag="LND56_002688"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK6744554.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1103..2023)
                     /locus_tag="LND56_002689"
     CDS             complement(1103..2023)
                     /locus_tag="LND56_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6744555.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2136..2888
                     /gene="xth"
                     /locus_tag="LND56_002690"
     CDS             2136..2888
                     /gene="xth"
                     /locus_tag="LND56_002690"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK6744556.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          877 a          631 c          540 g         1059 t
ORIGIN      
        1 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
       61 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
      121 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      181 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      241 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      301 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      361 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      421 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      481 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      541 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      601 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      661 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
      721 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
      781 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
      841 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
      901 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
      961 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     1021 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1081 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1141 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1201 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1261 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct
     1321 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1381 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1441 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1501 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1561 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1621 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1681 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     1741 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     1801 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     1861 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     1921 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     1981 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     2041 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     2101 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2161 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2221 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2281 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2341 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2401 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2461 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2521 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2581 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2641 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     2701 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     2761 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     2821 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     2881 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     2941 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     3001 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3061 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagat
//
LOCUS       DAFCLG010000184         3098 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFCLG010000184 DAFCLG010000000
VERSION     DAFCLG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.190"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..783
                     /gene="fabI"
                     /locus_tag="LND56_002691"
     CDS             28..783
                     /gene="fabI"
                     /locus_tag="LND56_002691"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK6744557.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(893..1906)
                     /locus_tag="LND56_002692"
     CDS             complement(893..1906)
                     /locus_tag="LND56_002692"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBK6744558.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2071..2991
                     /locus_tag="LND56_002693"
     CDS             2071..2991
                     /locus_tag="LND56_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBK6744559.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          905 a          629 c          597 g          967 t
ORIGIN      
        1 ttttatcaga aacaaaggag agtcattatg tcatttctag aaggaaaaaa aatcgtcatt
       61 atgggcgtcg ctaacaaaaa aagcattgct tggggttgtg ctgaagcaat gttgaaacaa
      121 ggggcagaaa ttatctatac ttaccaaaat gaacgaatga aaaaatcatt agtcaaattg
      181 gtcggcgaag aagcattcac tgtagaatgt gatgtttcta gcgacgaaag catcgaaaaa
      241 gcaatgaatg ccatcggtga atatgctggg gagatccacg gccttgtcca tgcagttgct
      301 tacgccaaca aagaagaatt gtccggaaat gtgtctgata tttccagaga cggctatcaa
      361 ttagctcaag atatcagcag ctactcttta attgctgtat caaaatacgc acaaccgttt
      421 cttgcaccaa acagcgggat cgttagcatg acttatctag gttcagaacg cgcaatcccg
      481 aattataaca tgatgggtat tgcgaaagct tctcttgaag ctgctatccg ttatttggca
      541 gctgagttct cgcctaaagg aattcgggtc aatggaatct cagctggggc aatcaaaaca
      601 ttagctgtca caggtgttaa agactatcaa aaactgatcc agttatctga atctcgtacg
      661 cctgatcagc aaggtgtgac gatcgaagaa gtcggtaatg cttgtgcttt cttaatcagc
      721 ccactttcca gcggaatcat cggtgatatt atctacgttg acaaaggtgt ccatctttca
      781 taaacttttg ttgcattctt taaagtaaaa catataaaaa tcaggaaaaa actttttaaa
      841 gtctcttcct gattttttct ttttacgttt ttttcaactc attactttga aattattcca
      901 cgacttcaat ccagccttct ggtgcttcta tgtctccaaa ttgaatacct gtcaattcat
      961 catacaaccg ttttgttact ggtcctactt ccgtttcact ataaaataca tgaagattgt
     1021 tattgtgata aatcccaccg attggagaaa taacagccgc tgtaccacat gcaccagctt
     1081 ctgcaaattg atctagatca tcaatgaaaa tatctccttc aattgcttgc agtcctagtc
     1141 gttcttcagc taactgcaac aaggaatatt tcgtgatact tggtaagata gagggcgatt
     1201 gcggagtgat gaattttccg tcttttgtaa ttccaaagaa atttgctgca cctacttctt
     1261 cgattttcgt atgtgtggct ggatcaagat aaacacaatc tgagtaatcc agttcttttg
     1321 cttctgttcc tggaagtaaa ctcgctgcgt aatttccgcc gacttttgct gcaccagtcc
     1381 ccttaggagc agcacgatcg taatcagata caataaaatt cgttggtttt agtccacctt
     1441 tgaaatacgc tcctactggc gtacagaaga cgacaaataa atattctgga gctggatgga
     1501 caccaatgtt ttcaccgacc ccaatcagta aagggcgcaa gtataatgta gcgcctgttc
     1561 catagggcgg cacaaaatct tcatttgctt ttaccacttg cttgacagca tcaacgaaca
     1621 tcttttcagg gaccattggc atcagcaatc gtctagcact ttgattcatc cgtcgactat
     1681 tttgttcagg tcgaaagaga ttgaccttgc cgtcttttcg acgataagct tttagacctt
     1741 caaaacattg ctgaccataa tgaagcgctg gagagccttc atgaatcgtc aggtaattat
     1801 cttctgtcaa ttctccttct tcccattgtc cgtctttcca tttggcaata aaacgccaag
     1861 gagttttgat ataagaaaaa cctagattgt tccaatcgat ttgcatatac ttaccacctt
     1921 cgttattttc tccacatttt atcattatcc atattatttg tcattttatt ttcagctttt
     1981 ttccttcatt atagttattt ttgctatttc cttttttgaa aacttcttgg tacaatacag
     2041 aaagaatcaa attttagaaa agaggatttt atgttttcat tattagcaac tgccggtacg
     2101 acagactctt ctggcgagac attgacagat caagctgtca gcaaagtaaa tgcgtttcag
     2161 cggttttggg acgggattga ttgggacgcc attttggctg cctttatcca aaaaagttta
     2221 tatctgattt ttttgatcgt ccttttttgg attcttcacc gtgtgggaaa atatctgatt
     2281 gataagtctt ttaatcagta ttctaaaaaa gccattctaa acgagagccg cttaaagact
     2341 ttacatagtc ttttaaatac tatttttcat tatacccttg gtttcttttt tatctatgca
     2401 attttatctg ctatcggtgt gccgatcgga tcacttttgg ctggtgccgg aattgcaggt
     2461 gtggcaatcg gtcttggtgc ccaagggttc atgagtgatg ttattaccgg attttttatt
     2521 atcatggaac aacagatgga tgttggggac tacatcaagc tagcaaactt aacgattgaa
     2581 ggaaccgtcg tatctgttgg tatccgtacc cttcagctga aagcaacgga tggtactgtc
     2641 catttcatcc cgaatcgaaa catcaccacg atcagcaatc tctctcgtgc aaatatgcaa
     2701 gtacttttag atattcggat tgttcctgaa gaaggatatg acaaaatcta tgagatcatt
     2761 gatcgagtaa atcgtacatt agctgaaaaa taccaagaag agctccaaac tgaaccttct
     2821 atctttggat tagtggatct tggaaacagc aattttgcga ttcgtacggt ctgctacgca
     2881 ttgaacggaa aacaatatgc cttaaaagaa gagttcttga gctcctatgt caaagaattg
     2941 acagcttctg gtttcacgat tccgaacagt ccaattgctg tcggaaaata atttggacaa
     3001 acgatgcaac gaatctcact aaaaagagcg tgggacaaaa gtaaagaaca cttttgtccc
     3061 acgctctaaa cacgtataaa cggcggaagc agaagcaa
//
LOCUS       DAFCLG010000185         2945 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFCLG010000185 DAFCLG010000000
VERSION     DAFCLG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.191"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..1235
                     /locus_tag="LND56_002694"
     CDS             147..1235
                     /locus_tag="LND56_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK6744560.1"
                     /translation="MKEERKLPEWLIDYAEKEGLIAELKPKHERQNFLVGDDRLDHAV
                     AFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKF
                     FESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEIC
                     VDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVE
                     PEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKV
                     CERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQ
                     LKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1246..>2945
                     /gene="topB"
                     /locus_tag="LND56_002695"
     CDS             1246..>2945
                     /gene="topB"
                     /locus_tag="LND56_002695"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6744561.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1078 a          519 c          653 g          695 t
ORIGIN      
        1 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
       61 gagtggaaaa tcttcatttt gagacagaaa agtacaaaga tcaccccgtt gattatcaac
      121 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga
      181 ttgattatgc agaaaaagaa gggttgattg ctgaactaaa gccgaaacat gaacggcaaa
      241 attttttagt tggagacgac cgtttagatc atgcggttgc ttttttatgg aaagatccac
      301 agacaggaga gacagtaggg gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg
      361 gtaaacgggg aacgtataaa cacattgaca agaatcctac gccaaatcac ggatttaatt
      421 taaaaatagg cgatccgaag catttaaaat tctttgaaag tagtattgat ctgttaagtt
      481 acgctgcatt gaatcgggag aaactacaag atgcctggtt agtttcgatg gacgggttaa
      541 aacatcatgt aatcagtcat tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa
      601 catttcctca atcgattgaa atatgtgtgg acaatgatcg tgcaggacat attttttatg
      661 aaaaagaaca gatgaaggga atcgttgatc cttttaccaa taagaaaatc cgttgtgagc
      721 gaggaattcc aaatgattgg caagtaccta aagaatacaa agctacttat gaagcagtcg
      781 caaaagaaat gagtgttgaa cctgaagcaa ttatggccat acacaaaact gaaactaatt
      841 tacagctgac aaatcaattg gtgtctgctc atgatgttca atctacgttt ggaaagatgt
      901 tggcgaaggg cgaaccagta gaaacaattg atttgaaaga agcatgtacc acggtcgcga
      961 aggaactgaa agtatgtgaa cgcgcagatg gaacatacaa ctttgatcgt ttttatagta
     1021 gaaaagccaa cataaaagac gttaatgcag gaatccttct ttcctataaa gcagaacaat
     1081 actacaaagg ctataaaaag catgaacatg aatttgtacc tgaagtgaaa aaggattgga
     1141 atgatcaatt gaagcatgag atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt
     1201 tgtttcaaca aggcagacaa caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt
     1261 ttagcagaaa aaccgtcaca agcgaaggct tatgccgata gcttttctaa agctacccga
     1321 aaagatgggt attttgagat ccaagatcga ttgtttacag gagaaactgt gatcacgtat
     1381 ggttttggtc atttagtcga gttagattcg cccgatatgt acgatgaaaa atggaaacaa
     1441 tgggcattag aacatttgcc tattttcccc aatcaatacc actatcatgt tcctaaagat
     1501 aagaaaaagc agtttagtgt cgtcaaacga cagctgcagt cagctgatac gattgtgatc
     1561 gcgactgata gcgatcgtga aggggagctg attgcctggt caatcattca acaagcaggc
     1621 gccaatcaag gaaaaacctt taaaagactt tggatcaact ccttagaaaa agaagccatt
     1681 tatcagggat tccaacagtt acgagacgca gaagagacct atccaaaatt tgaagaagta
     1741 caagcacgtc agatcgccga ttggttaatt gggatgaacg gtagcccact gtatagttta
     1801 ctcttgcagc aaaaaggcat accaggaagt ttttctttag gtagagtcca aaccccaaca
     1861 ctctatatga tttatcaatt gcaggaaaag attagaaact tccaaaaaga accatatttt
     1921 gaaggaaaag cccaagtaat cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat
     1981 gaaacccaag cgacacaaga agcatttgaa gcctatttaa aagaaaaagg tgttcaatta
     2041 ggaaaacagc caggtacgat ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca
     2101 cgtctttttt ctttatcgag ccttcagtct aaaatgaatc aactcatgaa agctagtgcc
     2161 aaagatactt tggaagccat gcaagggttg tatgaaggaa aatatctaag ctatccgcga
     2221 acggataccc cttatattac ggaaggggaa tatgcgtatc tgttggatca cttagacgaa
     2281 tacaagcatt ttttaaaagc cgaagcgatt ccgactccta ttcatacacc gaatagtcgg
     2341 tatgtgaata ataagaaagt ccaggaacat tacgcgatca ttcctacaaa aacggttatg
     2401 acagcagcgg cttttgaaca actaagtcca ctgcagcagg ctatttatga acaagtgtta
     2461 aaaacaacgg tggccatgtt tgcggaaaag tatacctatg aagaaacaac gatcttgacg
     2521 caagttcagc agcttcagtt aaaggcaatc gaaaaggtgc cgctagatct aggttggaag
     2581 aagttatttg gaaaagagag cgacggaaaa gagaaggaag aagagccgct acttcctaaa
     2641 gtgacaaaag gagaaatggt gacggtggat ctacaagttc ttgaaaaaga aaccaagcca
     2701 ccacaacctt ataccgaagg tacgttgatc actgcaatga aaacagctgg gaaaaccgtg
     2761 gatagtgaag aagcgcaatc catattgaaa gaagtggaag gaatcggtac agaagcgaca
     2821 cgggcgaata ttattgaaac cttgaaacaa aaggaatata tcaaggtaga gaaaaataag
     2881 ctcgtggtga cgaataaagg gattttgctt tgccaagccg tggaaaaaga gccgctgctt
     2941 acgag
//
LOCUS       DAFCLG010000186         2938 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFCLG010000186 DAFCLG010000000
VERSION     DAFCLG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.192"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..442)
                     /locus_tag="LND56_002696"
     CDS             complement(<1..442)
                     /locus_tag="LND56_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK6744562.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="LND56_002697"
     CDS             complement(500..1552)
                     /locus_tag="LND56_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6744563.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="LND56_002698"
     CDS             complement(1565..2503)
                     /locus_tag="LND56_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6744564.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          993 a          624 c          452 g          869 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatc
//
LOCUS       DAFCLG010000187         2934 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFCLG010000187 DAFCLG010000000
VERSION     DAFCLG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.193"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            204..1226
                     /locus_tag="LND56_002699"
     CDS             204..1226
                     /locus_tag="LND56_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK6744565.1"
                     /translation="MIAELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1237..>2934
                     /gene="topB"
                     /locus_tag="LND56_002700"
     CDS             1237..>2934
                     /gene="topB"
                     /locus_tag="LND56_002700"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6744566.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1069 a          528 c          641 g          696 t
ORIGIN      
        1 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
       61 gagtggaaaa tcttcatttt gaaacagaaa agtacaaaga tcaccccgtt gattatcaac
      121 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactacca gagtggctga
      181 ttgattatgc agaaaaagaa gggttgattg ccgaactaaa gccaaaatat gaacggcaaa
      241 atttcttagt tcgagacgat cgtttagatc atgcggtcgc ctttttatgg aaagatccac
      301 agacaaaaga gacagtaggg gcgagttatc aaggaacgtt cattgattat gaacgttttg
      361 gtgagagagg cacatataag catatagata aaaattcgac agcgaatcat ggctttaacc
      421 taaagatcgg tgaccctaag cagttaaaat tttttgagag tagtattgat ttacttagct
      481 atgctgcact gaatcgtgat cagttaaacg atacgtggtt agtctctatg gaagggttaa
      541 aacaccatgt gatcagtcat tattttggtg aggcagtatc tgaattgaga aagaaacaag
      601 cctttcctca atccatcgag atttgtgtgg ataatgaccg tgcaggacat attttctacg
      661 aaaaagaaca attaatgggc gcagtcgatc catttacgaa tcagaaagta cgttgtgaac
      721 gaggaatcgc taatgattgg caagtgccaa aagaatacaa aatcatttat gaagaagtcg
      781 caaaagaaga aaaggtcaca ccagaagcta ttatggccat acacaagaca gaaaacaatc
      841 tgcaattaac gaaccaactg gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc
      901 tttctgttaa cgattcaata gaagccatta atttgaaaga tatttgtaga gaagtcgcaa
      961 aagaattaaa agtttgtgaa cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag
     1021 aaaaagggga tattaatgcg cagattcttt tctcttataa agcagaacaa tactacaaag
     1081 gctataaaaa tcatgaacat gaatttatac ctgaagtaaa aaaggactgg aacgatcaat
     1141 tgaagcatga gatccagcaa caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac
     1201 aaggcagaca acaagaaagg gagtgaaatc gtcatgatga aaaccgttat tttagcagaa
     1261 aagccgtcac aagcgaaagc ttatgccgat agcttctcta aagccactcg aaaagacggg
     1321 tattttgaaa tccaagatcg attgtttcct ggtgaaactg tgatcacgta tggttttggt
     1381 catttagtcg aattagattc gcccgatatg tacgatgaaa attggaaaca atggtcatta
     1441 gaacatttgc ctattttccc tacacattat cactatcatg ttcctaaaga taagaaaaag
     1501 caattcaagg tggtgaaaca gcaacttcaa tcggccgaca ccattattat tgcgacagat
     1561 agcgatcgtg aaggggaact gatcgcttgg acgatcattc aacaagtagg cgccgatcaa
     1621 ggaaaaacct ttaaaagact ttggatcaac tccttagaaa aagaagccat ttatcaggga
     1681 ttccagcagt tacgagacgc aaaagagacc tatccaaaat ttgaagaagc acaagcacgt
     1741 cagatcgccg attggttaat tgggatgaac ggtagcccac tgtatagttt actcttgcag
     1801 caaaaaggca taccaggaag cttttcctta ggcagagtcc aaaccccaac gctttacatg
     1861 atttatcaat tgcaggaaaa gattagaaat ttcaagaaag aaccttattt tgaaggaaaa
     1921 tccgaagtaa tcgctcaaaa tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa
     1981 gcgacgcaag aagcatttga agattattta aaagaaaagg gggttcaatt gggaaaacag
     2041 ccaggcatga ttcgccaagt tgaaaccgag aaaaaaagcg cggcaagtcc acgtcttttt
     2101 tctctatcca gccttcagtc taaaatgaat cagctcatga aagctagtgc caaagacact
     2161 ttggaagcca tgcaaggctt gtatgaaggg aaatatctaa gctatccacg aacagacacc
     2221 ccttatatta cggaagggga gtatgcgtat ttgttggatc atttagatga atacaagcat
     2281 tttttaaagg ccgaatcaat cccaacccct attcatacgc cgaatagtcg gtatgtgaat
     2341 aacaagaaag tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg
     2401 gcttttgaac aactaagtcc actgcagcag gccatttatg aacaagtgtt aaaaacaacg
     2461 gtggccatgt ttgcggaaaa atatacctat gaagaaacaa cgatcttgac gcaagtccag
     2521 cagcttcagt taaaggcaat cggaaaagta ccgatagatc taggttggaa gaagttattt
     2581 ggcaaagaga acgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa
     2641 ggagaaatgg tgacggtaga tctacaagtc cttgaaaaag aaaccaagcc accacagcct
     2701 tataccgaag gtacgttgat cacggcgatg aaaacagctg ggaaaaccgt tgatagtgaa
     2761 gaagcgcaat ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat
     2821 attattgaaa ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg
     2881 acgaataaag ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacg
//
LOCUS       DAFCLG010000188         2598 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFCLG010000188 DAFCLG010000000
VERSION     DAFCLG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.195"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..483
                     /locus_tag="LND56_002701"
     CDS             46..483
                     /locus_tag="LND56_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744567.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            476..1183
                     /locus_tag="LND56_002702"
     CDS             476..1183
                     /locus_tag="LND56_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744568.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            1564..1743
                     /locus_tag="LND56_002703"
                     /pseudo
     CDS             1564..1743
                     /locus_tag="LND56_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1805..1989
                     /locus_tag="LND56_002704"
                     /pseudo
     CDS             1805..1989
                     /locus_tag="LND56_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            2023..2454
                     /locus_tag="LND56_002705"
     CDS             2023..2454
                     /locus_tag="LND56_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBK6744569.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          928 a          409 c          549 g          712 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
       61 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
      121 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
      181 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
      241 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
      301 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
      361 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcgactggct agatactttt
      421 gaaagtatcg aaaaaaatgg tgcattttat acgattgaat cggaggagaa agataatgaa
      481 taaggtggtt tatcagctaa caacagaaga tgtttcgcaa tttttaacaa gcaaagaaca
      541 cttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta
      601 ctttattcaa agagtggaag aacagttgta tatggaaagc gaaacaagtc aaacgaaggt
      661 cacagtcagc cataatcatt tagacgtgta taaacgtcgt cctgaaacgg taaaaaaagg
      721 aacagagctt ctacgactag aaacagaaaa atacatcatg aatgtgtacc tagaaaggag
      781 tgagctggtc gaaaactttg tcagctacac gatttgtagg aaacactatt actcagaatc
      841 agaatattat tcaattttcg agagcatgga attaatggaa gaagaaatgg aagagctaaa
      901 cagcaaaaat gcgacggtag acgattatta ccttgtattt actaggtttt tgacgtgtgg
      961 gacggaatct atcgtgttaa tgggaatgga ttatgaactc gctgtacccg aagcattttt
     1021 gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga
     1081 ttttttagac acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa
     1141 acgaatgcca tatcaacgat ttgaaaggaa aaaaggtcaa taaccattta taccgaaaag
     1201 tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt cttatttcgc
     1261 accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataagcggg caaagcccga
     1321 taacctaaga gaaagaagga gatccatatg aataaaatca ttcctctatt gttcgtactg
     1381 tttgttggcg agatacttat gcgtcatgct gcagctgtac atttaaactt cgtagtattc
     1441 ttaggaatac tggtattttg tttgatcttt tggcgatttg tattagccct tgtgagtcta
     1501 ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa ggagagagta
     1561 ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga cgttggatga aaagagacaa
     1621 aaagaattgc agtaaccaaa gcagaagtac ggcaaaagtg aatccaggat tatgtgtatt
     1681 gcgttagatt tattgattgc gcaagaaaaa gcaggatttg acataccagc actcaaaaag
     1741 tgatcgcact ttgcgcacgt gttaatacgt gttataatta ttttgtaagg aggtatagtg
     1801 cacaatgcca cttacgggaa aagaaatgct taagttattg aaaaaaatgg ctgggttgag
     1861 cgaaggcaag agggatttca tcatcatctt tataaggatg gtgttagaat cactgttcct
     1921 gttcatgcca atcaagatct tggccgagga cttgagcgaa aaattttaaa ggatgcggga
     1981 ttgaaataat ctcgcattcg attaaagaaa ggattgactg aaatgttaag tgagccttta
     2041 gcaaaaacct atcctgctat ttttagccca gaagaaggtg gtgggtactt tattgaattt
     2101 cctgatgttc aaggtgctta cacaggaatt aacgaagacg atatatctta tggaattgct
     2161 atggcacaag aggtgttagg tatggtttta gcagattata ttgagcatga agatctactt
     2221 ccagaaccaa caccaatcaa taagatttct gttgaagatg attcttttac tactttaatt
     2281 cgtgtagatg ttgccaaata tttgaaagac acagaacttg tcaaaaaaac cttaacaatt
     2341 cctcaatggg cggataagct aggcaaaagg gctggcataa atttttcagt ccttctaaca
     2401 gaatcaattg cagataaagc agataacatt cttcgttctg gaagaaataa ttagaactca
     2461 aaagggtcag aaacttaaaa atctttggct tcttttttta ctacaaagat ggcttcttta
     2521 aattatttgc ctgattgatt taaaaaaatt gcgcaaagac tacgagctaa ttaagttgct
     2581 cgtggtcttt ttttatga
//
LOCUS       DAFCLG010000189         2478 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFCLG010000189 DAFCLG010000000
VERSION     DAFCLG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.197"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            195..641
                     /locus_tag="LND56_002706"
     CDS             195..641
                     /locus_tag="LND56_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6744570.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            914..2266
                     /locus_tag="LND56_002707"
     CDS             914..2266
                     /locus_tag="LND56_002707"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBK6744571.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          884 a          391 c          493 g          710 t
ORIGIN      
        1 ttaaagatga aaagaacaca caaaaattta tgatgagaaa aattatttga atagagacgt
       61 agaatagtga acgcgactgt tttacgtctt ttttcattaa aatacaataa aattgaattt
      121 tcttttcatg ttaagtggat tttaaaatgt aaaaatagta tgataagacg agaataacaa
      181 cgtggggtgt ttacatggaa aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag
      241 agaacattcg aatcacacct caacggtacg cggttttaga atatttgatt gaacatcatt
      301 cccatccaac ggctgatgag atttataaag cacttgaaca tcggtttcca aatatgagtg
      361 ttgcgactgt ttacaataac cttcgcttat ttacttcgat tggatttgtt caagaaatga
      421 agtacggcga tgcttctagc cgatttgatt tcagttcaaa gcgtcattat catgtaattt
      481 gtgaaaactg cggtaagatt gttgattttc attacccagg gcttgaagat atagaaatgg
      541 ctgcaagcag actgactgat tttgagatta atgaacatcg gttggaattg tatggactct
      601 gtccggattg taaagagtca gcaaaaaagg cgcaaatgta aaaacagttt ctacaagtgt
      661 tatacctaaa aaaagctagc tgtgtaaaaa aaacgctagc ttttttattt ttttgtcttt
      721 tattcacaat cttagatata aaagtgaata ttattaaaat aagaatcttg tagatattgg
      781 gttcttatgc ttgaaaagga atgaaactac aaacaaacct ttgtgaattc acaagcatta
      841 aactgttgta tccgttttat ttctgttata gtagttttgt cgaaggacaa aatgttaatc
      901 ggagggacca taaatgaaag tagtagtaat tggctgtact catgcaggta ctgcagctgt
      961 aaaaagtatt ttaaaaaatc atccagaagc agaagtgaca gtatatgaaa gaaatgacaa
     1021 tatttctttc ttatcttgcg gtatcgcact ttatgtcggc ggagtagtaa aggacccagc
     1081 aggcttgttc tactcaaatc ctgaagaatt atcttcatta ggagcaacag ttaaaatgga
     1141 acacgatgta acagacgtcg atacagaaaa taaaacagta acggcaaaag atctgaaaac
     1201 aggagaaaca gaaacggttt cttatgataa attagtgatg acaactggtt catggcctat
     1261 tatcccaccg attagtggaa tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca
     1321 agcaaatgaa attattgcac aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg
     1381 atatatcggt attgaattag tagaagcttt tgtcgaatct ggaaaacaag tgacattgat
     1441 tgatggatta gatcgcatct tgaataaata tctagataaa ccattcacag atatcttaga
     1501 aaaagaattg acagaccgcg gtgttactct tgcgctaggc gaaaatgtta ctgaatttat
     1561 cacagatgaa gaaggaaaag tgaaacaagt agctacacca acagatacat ttgatacaga
     1621 tatggtcatt ttatgtgtgg gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga
     1681 tacactgcca aacggggcaa tcaaagtgaa tgaatacatg cagacaagta atccagacat
     1741 ttttgcagct ggagactctg cagtagtcaa ctacaatcca agtggcacgc aaaactatat
     1801 tcctttggca acaaatgccg ttcgtcaagg tttgcttgtg ggaaataact tgacagatca
     1861 taaaatggcg tatcgtggta ctcaaggaac ttcaggactt tacctatttg gctggacgat
     1921 cggttcgaca ggtgtgacca aagaaagcgc aactttgaac gatctagaag tccaagcgac
     1981 tgtatttgaa gataattatc gtccagagtt catgccaact actgaaaaag taatgatgga
     2041 gctagtctat gaaaaaggaa cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga
     2101 cattacacaa tcagcaaata ccatgtcttt agctgttcaa aataaaatga ctgtagaaga
     2161 tctcgcactt tcagatttct tcttccaacc acattttgac cgcccatgga attacttgaa
     2221 cctacttgca caagcagcgc taggcgaaat cgaaactgca caataaagaa aaagggttgt
     2281 gacacaattt tgtcacgacc ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc
     2341 ttcgaatttt ttgtcacaat aagcagcaaa gataaagctg ttacataaaa gaacaagcag
     2401 ctttatttga ttttgaaaag aaaggtgaga aagaagatga ataaagaata ttttgtccaa
     2461 caagatgcat ttttagaa
//
LOCUS       DAFCLG010000190         2464 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFCLG010000190 DAFCLG010000000
VERSION     DAFCLG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.198"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..299
                     /locus_tag="LND56_002708"
     CDS             87..299
                     /locus_tag="LND56_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744572.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(577..2376)
                     /locus_tag="LND56_002709"
     CDS             complement(577..2376)
                     /locus_tag="LND56_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6744573.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          874 a          444 c          456 g          690 t
ORIGIN      
        1 gaataaaaac gttttcaaat acaaggtaat ttgttattat aaaataaaga aaaaacaaaa
       61 taaaaataaa gaaaagaggg aatgaaatgg taaaagcaga agtaggaaga tcttatatat
      121 gcaaaccgat cggagtatct catgaactaa tcggctgtat tgaacgtaca tatatcaaca
      181 cggctgtcgt gtatgttgaa agttatgacc aacgagacta tcaacttatc aatgaaagca
      241 agtatcgcat gctcgtaaaa ttctgtgata ttagcgcacc tactgaattg gtttcttaaa
      301 cagtattttt gagaattcgc aagaactccc ttataatata aataaagaca gaagggagcg
      361 agaagatatg ccaaaaagaa aaagttcagg gaaaatggga attattcttg cagtgatctc
      421 tgcaattcct gctattttag ctattgttcg tgcgatgaag gaatcaagag aaagttaagc
      481 acacattatg agcgtcaaac aaaaaacgca tatacctgcg gtggcaggtt atgcgttttt
      541 tgttgtataa agactttttt cattaacgat ttttgattag ccgactccca aaacaaaatt
      601 tagcagttta tctgaataag tgatttcttc atctgtttgt ttcacacttt catttagtag
      661 tcccatctca tcggttatga taccatccac accataaaac atcatgcgac tcatggcatc
      721 tgaatcgttg attgtccata catatacttc ttttccgtct tggtgggcag aattaatgaa
      781 gttacggttg atcgtactat attccattgt cagaaaatca gcaggcgtca cagggggccc
      841 aacaatattg aaaggtaaga tgtagcccac ataaaagttt ggttctgctt cttttaaatc
      901 tgaaacggtt tggtacgtca aactttgcaa aatatgatgg ttctctaaga taactttgcg
      961 gtatttatgg acaaatcgtt cgacaaggtc atccgaatct tttttagagg tcttgatctc
     1021 aataagtaat ttttggttta gcttatgtgc tttttccaaa taagaatcaa aagaacaaac
     1081 aggggcttgc actccgtttt cagtaactgt aagatttgtc aattctttca atgtaagatc
     1141 ctgtggtgtt ccatttaccc ctgttaagtg acgaagattt gcatcatgca tcactacgaa
     1201 atgaccatct tttgtttcct gaacatccat ctctacataa tcaggatgat agtctctgct
     1261 tgttttttca agtgcttcta aagtattttg cgccccattt tgggtactca cacctctgtg
     1321 agagacagtc actggttttg tctgaacagc tgaagtcaga taatccatat tgtaaagaca
     1381 gacgccaata ccgaaaaaaa ctgcaaaaag agtcaaacca gtgtttttca gcgcagagaa
     1441 acgcaagttg ggggcttgtg ggataaacca cttagggatc tcaggcaaga agccttcgtc
     1501 atccatgaaa tcaacaatga tataaaaaat ccctacggta gacatcacga tattgaataa
     1561 taaaatccct tgtaaaagcg tcatagcaaa cacagcgctg atcaatgcat agtctgggaa
     1621 aaattgttca accatacttt gtgccaaaat aacaacgata tatcctaaag aagaaaggag
     1681 taaaatcgta ccgccaatca caataaattg gcccgtgatg gccaaaagac gcgatttggt
     1741 caatgaccag ctttcccgaa tggctgctcg aaaagaacga tctcgtagaa tcatttccgg
     1801 taaagcaaaa atcagtcgaa tcccaatata gccaaggaaa atataaacga gtaaaaagga
     1861 actgacgatg atccaacgat tagcaaaaat aaaatccata atgaaagcgg ggatttttat
     1921 ttttgacagt agatcagaat taaaacttaa tccgctgatc ggtagaatca ataaaaaata
     1981 agcaaggaaa aacaaaaaag taatgggtct cactttcttc agctgaagta tcgtcaagtg
     2041 taagagttgc ttcaaagaga ttggttcttt ctttttaata aaaaaaacac tcatcaaaag
     2101 aaaagtaaat tcgaaataaa ctaacagtaa aatcgtcatc aaaactacga ttaacgagag
     2161 tagaacccca ggatgttgag aaaaaattga aggaatattg tcataagaca aatagtcaat
     2221 tgcccctcgg tgtaaaataa aacgggtcgt actgctcaat aagggtaaac aaataaataa
     2281 aataaagcca tgcatcagca agacatcacg gaaataagct tgtgttcctt ttaaaaagtc
     2341 ccaagcatta cctaagctac gtttcaaata attcatccta tcacctgctc ttcacattgt
     2401 agcgcaaatc taatgaaaaa ggaaagggtg cagtgtgttt tgcagaccct ttcccagatt
     2461 taac
//
LOCUS       DAFCLG010000191         2385 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFCLG010000191 DAFCLG010000000
VERSION     DAFCLG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.199"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            306..560
                     /locus_tag="LND56_002710"
     CDS             306..560
                     /locus_tag="LND56_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6744574.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            561..878
                     /locus_tag="LND56_002711"
     CDS             561..878
                     /locus_tag="LND56_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6744575.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1437..2372
                     /locus_tag="LND56_002712"
     CDS             1437..2372
                     /locus_tag="LND56_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744576.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          853 a          338 c          453 g          741 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaatggatgt
      121 taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta tatgttttca
      181 gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct taagaaacac
      241 cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa aaggagtgtg
      301 ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga gtgatccttc
      361 caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa ggcaacttat
      421 tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt gaagaaagtt
      481 tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa gctttcggaa
      541 aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa agttttcaga
      601 ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct ccagccagca
      661 taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat tttccaacta
      721 tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg atcgtgatcg
      781 gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata cttgtaggac
      841 gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga aaaagtcgct
      901 catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt cggttactcg
      961 aagattgata tataagactt agtacctttt tattttgctc catgttatgg ccgaaggtct
     1021 aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg gggatcgctt
     1081 ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc tctattccca
     1141 aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc aaaattgacg
     1201 taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt taaaagagga
     1261 agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa aatatacatc
     1321 cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa aggaatggga
     1381 taaaatatag gtacaataac tatttgatgc taaatataat taatggagga gagatagtga
     1441 acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt actactttca
     1501 aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt
     1561 ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt ctttctgaga
     1621 gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt gatatatata
     1681 cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc agtgaatatc
     1741 tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta ctaccatatt
     1801 tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca ggtaatcaag
     1861 gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg atgggttacg
     1921 gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct gggcgttcat
     1981 tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag ttagaaaaca
     2041 tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt gaagttttga
     2101 aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata aaagcacatt
     2161 ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga gggaaaatca
     2221 atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta atgataacag
     2281 atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata aaacttgtta
     2341 ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAFCLG010000192         2373 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFCLG010000192 DAFCLG010000000
VERSION     DAFCLG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.200"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            217..>2373
                     /gene="acm"
                     /locus_tag="LND56_002713"
     CDS             217..>2373
                     /gene="acm"
                     /locus_tag="LND56_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBK6744577.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYK"
BASE COUNT          939 a          356 c          457 g          621 t
ORIGIN      
        1 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
       61 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      121 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      181 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      241 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      301 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      361 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      421 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      481 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      541 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      601 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      661 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      721 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      781 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      841 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
      901 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
      961 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1021 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1081 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1141 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1201 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1261 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1321 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1381 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1441 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1501 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1561 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1621 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1681 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1741 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1801 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1861 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     1921 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     1981 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2041 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2101 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2161 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2221 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2281 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2341 atctgtgctg ggatattctt acaaaaatat aaa
//
LOCUS       DAFCLG010000193         2359 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFCLG010000193 DAFCLG010000000
VERSION     DAFCLG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.201"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            71..2224
                     /locus_tag="LND56_002714"
     CDS             71..2224
                     /locus_tag="LND56_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBK6744578.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
BASE COUNT          805 a          416 c          525 g          613 t
ORIGIN      
        1 ttttttcttt tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga
       61 aggataggaa atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa
      121 aataagaaaa ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt
      181 tcgcttaaat tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt
      241 gggttatttg cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac
      301 gattacggtc caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt
      361 agttacgaat aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa
      421 agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa
      481 tctgacagat agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc
      541 cacaaaaaga ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat
      601 ggtcaataaa gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac
      661 catctttaaa cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg
      721 tgttactgat caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc
      781 gactggcaca gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg
      841 cagtgtgacg actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa
      901 aggttattct cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt
      961 cctgcaagga accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa
     1021 tcaaaaagaa atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga
     1081 attccaatca aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg
     1141 caaagcacag tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt
     1201 gctagcaatg actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt
     1261 aggcacgatt actagcgcct ttgctccagg gtctgtagtc aaggctggta ctttgacagc
     1321 aggctgggca agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct
     1381 gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc
     1441 agtcaaagca ttggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt
     1501 aggactagac tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta
     1561 tcagcaattg agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt
     1621 gccgaatgaa tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga
     1681 taatggcaaa gaatggtata ctccaggtaa ctttaccgat ttggcatttg gacagtttga
     1741 cacatataca ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg
     1801 agtacagccg cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga
     1861 agtgaagaaa gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat
     1921 ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacagcagc
     1981 caaaccatta gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt
     2041 tgcggaaggt catccagata ttacgacagt caatagtaat atcgtggcat acggaccaac
     2101 agacaatccg gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca
     2161 catgaacttg acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa
     2221 ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa
     2281 atagcttctc acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac
     2341 cgtttactcg gcaaaaaag
//
LOCUS       DAFCLG010000194         2313 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFCLG010000194 DAFCLG010000000
VERSION     DAFCLG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.202"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            120..1982
                     /locus_tag="LND56_002715"
     CDS             120..1982
                     /locus_tag="LND56_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6744579.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1982..>2313
                     /locus_tag="LND56_002716"
     CDS             1982..>2313
                     /locus_tag="LND56_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6744580.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          833 a          343 c          454 g          683 t
ORIGIN      
        1 atagataaac tctgtaatat tagttaaaaa gctataagtt gcacttagtt aagtgcaaac
       61 atttcatatt gtcgttagtt tagaaaacgc ttatattagc aatgtaagga gagatgataa
      121 tgcagcagct aacaaaaaga gaaaggcagc ttttgattta cttggtggaa atgtctgaac
      181 cggtaacaat caaaaagttg gctgaaaaag aaaatgttag tgttcggacg ataaaatacg
      241 atctagatga tataagagag tggctgaatg agcggaagca agtcctatat agcaaacgta
      301 gtcagggaat ttggatgaat attagtagct ctgatcgaat caaattaaaa agcgaattga
      361 tggatgttaa tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa cgtttactaa
      421 ttatgctttt agtaacaaaa gaagctatta ctggaatgaa aatggccaac gcattagatg
      481 tcagcaaaga tacgatcatg aatgatttag atgtgctgga aaataatttt cttaaggaag
      541 gattgacctt gaatcgtcag gcgcgaaagg gtttttggat cataggagaa gaacgtctag
      601 ttcgattaac gattgaagag attcttcaaa aagagttcac ggattatgac atttataaat
      661 taatgtcatt gttattgaat ggcggtgaga ctgaatactt tgaaatgtat tctgctactg
      721 ctacacctat ccaagaggta tttaatcaag taatcattcg aatgcgacac ttactggaat
      781 ttgaaaatct agagaaactg aattacgcgg aattactaaa tattctgatt cgagtgacaa
      841 ttgctaccgt tcgcttacgc aaagaggcta cgattggtcg ttatcaattg gttgctgaac
      901 aagaattaga caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt gattattatc
      961 agttgccact gttcgaggat gaatatcatt atatctatag tgatacattc gacacgattc
     1021 accagcaaga cgtcatgaaa ctagttgaga atgtgattta taaagtaagt aaagcaatca
     1081 accattcttt ttatcaagat ccacaattgt tgacgaatct gtatgctcat ttatccatgc
     1141 gattatcgag aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa gatatcaagc
     1201 agaaataccc ggaattattt gatgcgatca agcaagcagt tcgacaagaa ataagtgaac
     1261 aaaatattct gatcaatgat tctttcgtgg catatattgc cttacacttt ttggtttcct
     1321 acgaaagaga agaagatcta cgaagcgtta gagccgtcta catttgttca acagggttgg
     1381 gtgtgactag tctgatcaag caaaagatca atgaagaatt aaataatatc actatcgtgg
     1441 catttgcttc agtattgaat gcgcaagaaa tgattgctac caaagatcct gatttggtga
     1501 ttagtatttt tccaattaat ggcatcgatc gtcctttcat caaagtccat ccattaccca
     1561 ctgaacagga cttgaaaaaa attcaacaaa taataaaaaa aatacttgct tcttccaatc
     1621 agcaggaaag gcataacttg aatcttagag aaacgtccat caataaagat actttagaag
     1681 actttagcaa ggaattgatt ttgaaagctt atacgattta tgaacgttta cttgtcattt
     1741 tggaagcaga attgatccaa gaatatcgag aagctttttt gcttcatgta ttcttaatgg
     1801 ttcatcgtat cacatttgat caacaatata tggcagaagg tcgtcaagta gaccagatta
     1861 ttttagacaa aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa aataatttag
     1921 gaatcaatca gtcagaaatc agtgcattat tctcatatat aaaagaagga gcgtcaagtt
     1981 agtgaaaatg tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc gggaaaagtt
     2041 aaaacaagtg attgatctaa ttgaagagcg attagtagaa catgctattg taccaaccga
     2101 gttgcagtgg accattctaa ttaatcactt gaatgagatg ctgaaacgtt ctcaaagaaa
     2161 ggaaacgatt cccgtagtcg atcgggattt gttcaaagaa gtatccaaag aagccataac
     2221 tatttctgat gaaatagtta gaagtatcgg taatttaacc gatgatgaaa tatatgtttt
     2281 gtcgattcat tttgaaacag caaaaaataa tta
//
LOCUS       DAFCLG010000195         2220 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFCLG010000195 DAFCLG010000000
VERSION     DAFCLG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.203"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(51..1598)
                     /locus_tag="LND56_002717"
     CDS             complement(51..1598)
                     /locus_tag="LND56_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK6744581.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="LND56_002718"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="LND56_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK6744582.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..>2220)
                     /locus_tag="LND56_002719"
     CDS             complement(2043..>2220)
                     /locus_tag="LND56_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744583.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          622 a          465 c          397 g          736 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
//
LOCUS       DAFCLG010000196         2086 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFCLG010000196 DAFCLG010000000
VERSION     DAFCLG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.206"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(580..945)
                     /locus_tag="LND56_002720"
     CDS             complement(580..945)
                     /locus_tag="LND56_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744584.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1021..1266)
                     /locus_tag="LND56_002721"
     CDS             complement(1021..1266)
                     /locus_tag="LND56_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744585.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1336..1590)
                     /locus_tag="LND56_002722"
     CDS             complement(1336..1590)
                     /locus_tag="LND56_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744586.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1754..2077)
                     /locus_tag="LND56_002723"
     CDS             complement(1754..2077)
                     /locus_tag="LND56_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744587.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          773 a          358 c          370 g          585 t
ORIGIN      
        1 gaatatttag aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt tattatttta
       61 gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg
      121 ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact
      181 gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat
      241 cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga
      301 aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt
      361 gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt
      421 atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag
      481 gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt
      541 taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt
      601 ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg
      661 cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat
      721 aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag
      781 ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg
      841 tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg
      901 ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa tgtctccttt
      961 atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa
     1021 tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta
     1081 aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt
     1141 acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg
     1201 gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat
     1261 actcatttaa tcacctttca ttatagtaga atagttataa tgactataga aagctacaca
     1321 tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa
     1381 taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt
     1441 gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa
     1501 ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac
     1561 ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata
     1621 aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata
     1681 gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct
     1741 gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat
     1801 ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg
     1861 aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga
     1921 gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa
     1981 ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata
     2041 aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataa
//
LOCUS       DAFCLG010000197         2082 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFCLG010000197 DAFCLG010000000
VERSION     DAFCLG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.207"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..971)
                     /gene="cysK"
                     /locus_tag="LND56_002724"
     CDS             complement(45..971)
                     /gene="cysK"
                     /locus_tag="LND56_002724"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK6744588.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1111..1998)
                     /locus_tag="LND56_002725"
     CDS             complement(1111..1998)
                     /locus_tag="LND56_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6744589.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          578 a          468 c          353 g          683 t
ORIGIN      
        1 taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa
       61 agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct
      121 tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct
      181 actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta
      241 atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg
      301 attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt
      361 tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg
      421 atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct
      481 tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat
      541 tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca
      601 gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa
      661 cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact
      721 cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct
      781 ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt
      841 acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt
      901 acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag
      961 atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct
     1021 gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata
     1081 tatggtaatt tctttgcctt tttatctaaa tcaaacgttt agttcttgac caactaataa
     1141 agaataactg gacatagaca tgccattttt cgccattaga tcatcaacag acatgccaaa
     1201 tttttgtgca atatcccata gcgtatcacc tgacacgact gtatatgtct gaccattatt
     1261 tgtatttaca tctgttgttg aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt
     1321 tgtacttgtg gttgttgtag ccgaacctgt tgatggcgta tcaaaacggg ttaaattata
     1381 agtattaatc aatgagttca atttggcccc ataatttgga tcggtagcat aacgacctgt
     1441 caagtacgct gttgcatcat aaaaacttgt tgtattgctt ttccacacgc cagcataatg
     1501 ataatcgccc gtggaaaaac tggttgaacg aagaacattc gcatggtctt ggaaggattc
     1561 ccaataagaa gaatattttc ggaatggctc attcatcgtt acccattgtc cgtttacata
     1621 ttctttggtt gccatataaa cgacctgtcc gttatagctt cctttcacac cgaatagatt
     1681 ataatttggc gcactagcta atccggaagt gccatatgca ctttccaaca acgcttgagc
     1741 gatcatgact gaagcataca agtcattact tgcagcaact tctgacgcag accagccgat
     1801 ttgatcgata aacgcttgag gatcagcgac tgtttgagat ccttgttcat ctgcatgaac
     1861 atgttgcggt accgctgtaa ccatcatcgg cccaactaaa agactcgttc caattaaagg
     1921 agcaatcgct tttttattga tcaccatttt tcttttaggt ctatggtgcc gggcactgcg
     1981 ggtcttcagc tcttgcattc aaattcctcc tcaaaataaa aaaacttaat aaaaagttct
     2041 cttcttaaaa caaacttaat taattaaatt atacttttaa tt
//
LOCUS       DAFCLG010000198         2000 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFCLG010000198 DAFCLG010000000
VERSION     DAFCLG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.208"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..376
                     /locus_tag="LND56_002726"
     CDS             <1..376
                     /locus_tag="LND56_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK6744590.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="LND56_002727"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="LND56_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK6744591.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2000
                     /locus_tag="LND56_002728"
     CDS             1857..>2000
                     /locus_tag="LND56_002728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744592.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          552 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt
//
LOCUS       DAFCLG010000199         1990 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFCLG010000199 DAFCLG010000000
VERSION     DAFCLG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.209"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="LND56_002729"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="LND56_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6744593.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="LND56_002730"
     CDS             852..1040
                     /locus_tag="LND56_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6744594.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="LND56_002731"
     CDS             1053..1610
                     /locus_tag="LND56_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6744595.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="LND56_002732"
     CDS             1642..1884
                     /locus_tag="LND56_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6744596.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          307 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc
//
LOCUS       DAFCLG010000200         1954 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFCLG010000200 DAFCLG010000000
VERSION     DAFCLG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.210"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="LND56_002733"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="LND56_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBK6744597.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          699 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaa
//
LOCUS       DAFCLG010000201         1932 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFCLG010000201 DAFCLG010000000
VERSION     DAFCLG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.211"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..1167
                     /locus_tag="LND56_002734"
     CDS             202..1167
                     /locus_tag="LND56_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6744598.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1367..1570
                     /locus_tag="LND56_002735"
     CDS             1367..1570
                     /locus_tag="LND56_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744599.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
BASE COUNT          640 a          288 c          436 g          568 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttagatt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 ttctttgagg aggaacaaaa tttgagtaaa aatggcataa tcgtaaatga gtgggaaaat
      241 ttaacagccc tatctaacag gcggttaaat gttcaaaatt tgaacacccc cctaaaatta
      301 cagtggtcaa ttgaccgaat tactatcgtt ggaaaactga aggaaaatat ttattatcac
      361 actcaaaatg atgtgttgat tttagatttt gagcaattaa tgcgggtgaa tgaaggcaat
      421 ggctatttaa aagcggttgg gaataacggc tggcaattat tagaccaata cgaagaaaat
      481 attgcttata ttgagatttt gaaatggcaa gatggcaaag ggcggattga ttttaacccg
      541 aataagattg gtcatttttt agctagttca atgaaaaatt tcattcatga tttatttcta
      601 gaaccacatt tctcacgtgc ggatattgct tgcgatatta tcgatgtgcc tgatgagttt
      661 attacacaat atcgagtggt tgatcctgtt tctttcaagc caatttatgg gcggtctggg
      721 aagctggaaa cagcttattg gggcagtcgt gccagcgaac gacaaattcg gctatacaat
      781 aaaaaattgg aacaagaaac caaacgaaaa gttgtaccaa aggaagttga gacttggtgg
      841 cggttagaaa tgcaactacg gcgtggcaag gcaaccgatt ggcataatat ggttcaagaa
      901 agtttgaata gttttgcgag cctgcatttt ttgccgattg atacaacacc tacagataga
      961 ataatgctcg ctggtttaat gtctgatcaa aatttctggt ctcttttgtc gaggaattca
     1021 aaatataaat atcgggaatt gctgaaagaa gaaagtcaaa atgatgaatt gacgaatcat
     1081 ttgcgagaaa cgtttgcaga atcggcagac aatttgaaac aagaattaga cacgtggctt
     1141 ttgggtttgg acgtgacaga gaagtgatac cgaataagaa aagtcagatg agtgagagta
     1201 aatttacgtc aatgtttaat gacgtgagtt tacgataagc gattattttg cgtgaagcaa
     1261 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     1321 ttgtgacagc gaataccctc ggagagcatt tggaattgtg acagccatgt cacaattagg
     1381 aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1441 cgcgaaggag gggtttaact tggggtttag tatttcgttc aaaaagggaa caaaaaatac
     1501 aagcatttct cataataatc gtgagttaac tgatagccaa aaaattaata gtttgtctgg
     1561 tagaaaataa ttagcagatt tacaagtggg aaataaaaag ctgaaagaag aaaattgcat
     1621 ttcaaactac attttctaca ataactctaa aatggcatat tttgcgttct gagcggtttt
     1681 agcgtttatt tcgtttagtt atcggcacaa tcggtgaaac acgttagagg agcgaaaatg
     1741 gagcctgtaa ggcgactggt agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa
     1801 cgaaataaat gctggcagcg ccattctatt tcggcaggag gaggctcaag gggttcgggg
     1861 aatgaaattc cctgaggtct ttttattttt tctagaggag tgctataatc aaagaaaatg
     1921 agaaaaaaga gt
//
LOCUS       DAFCLG010000202         1915 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFCLG010000202 DAFCLG010000000
VERSION     DAFCLG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.212"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..280)
                     /locus_tag="LND56_002736"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="LND56_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="LND56_002737"
     CDS             complement(546..740)
                     /locus_tag="LND56_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744600.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="LND56_002738"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="LND56_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          668 a          267 c          251 g          729 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttctcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tattt
//
LOCUS       DAFCLG010000203         1729 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFCLG010000203 DAFCLG010000000
VERSION     DAFCLG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.213"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            193..390
                     /locus_tag="LND56_002739"
     CDS             193..390
                     /locus_tag="LND56_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744601.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            496..978
                     /locus_tag="LND56_002740"
     CDS             496..978
                     /locus_tag="LND56_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744602.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1036..1632)
                     /locus_tag="LND56_002741"
     CDS             complement(1036..1632)
                     /locus_tag="LND56_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6744603.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          531 a          266 c          351 g          581 t
ORIGIN      
        1 aggaatatgt tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt
       61 gaaaacgaaa acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat
      121 caatgagtta aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt
      181 aggaggattt tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca
      241 attggtttta caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg
      301 gtcaaagttg cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg
      361 gttgttgcaa aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca
      421 agtccaattt ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa
      481 gcgaggcgag ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg
      541 atgtttttat ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga
      601 tgggtctact tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt
      661 catcctacag cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt
      721 ttgttggaca gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa
      781 ggtgttcctt tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc
      841 gtaaaaatga attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca
      901 ttagaagaat tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt
      961 atgattgatc cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt
     1021 ttatttcaaa taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt
     1081 ttccccagcc ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga
     1141 agggatattc cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt
     1201 tgcgcccaat cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga
     1261 ttgttctgac caatctggga agttgtctgc atataattca aattgttctt gtagaccttt
     1321 taccacgtca gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa
     1381 tttgttttgt gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt
     1441 gatttcccaa agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc
     1501 attgaaagca gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt
     1561 agaaagtgaa acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt
     1621 aaaagttgtc ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta
     1681 cgaaatgaag tatagcacct tacaaaaagt aagtaaacag atatgctca
//
LOCUS       DAFCLG010000204         1677 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFCLG010000204 DAFCLG010000000
VERSION     DAFCLG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.214"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            149..1627
                     /locus_tag="LND56_002742"
     CDS             149..1627
                     /locus_tag="LND56_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744604.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          613 a          230 c          302 g          532 t
ORIGIN      
        1 tttgcaatat tctcaaaaaa aagataaaaa atgagaataa ctgatgaaaa aacaagcata
       61 atccatcata aaatcagtat aggtgaaaag atgaactttc atctgcatat caagtaattg
      121 gtatgaggca ttttttgagg tgtaggatat gaagttggat taccttatag aagaggaaga
      181 tagagaaaaa ctgctgctag ctagatattt agaatttgag aaaaagaatt atgtagtgct
      241 gcctaagtta tatgaaacat taggtttgtc taaatttaaa acaagaaatt atatcataga
      301 attaaataaa gaatttactg aattcaaaac aaagccagag atccaaattt cagaagaagc
      361 agagatccat gtcgaacgtc tgaatttgac ggatatcaaa aatttaagaa gaatctactt
      421 tcaacgatca aaaattgctc gactgttaac ggagatcgtg gattcaaaag taacgatcga
      481 aaaatttgca gagaatcatt ttttaagcat atcaagagcc tatgtaaaaa gaagagaatt
      541 agtctctttt cttgaacagc atcaaataaa gttaaagaaa aacatattag ttggggaaga
      601 aataaacata cggaacgtat tatttggggt atatttcggg gtctttaatg gtttttctat
      661 gccatttaga gaaagcgtca tgaatgaagc aaagagtatc gtgcaatatt ttgaatatct
      721 gttcaattta aaactgtctg caacaaaacg aatcaaattc aatttgctaa ccactattag
      781 tttgttgagg gcctatggga atcaaccaat cggaaaacgt ttcttttcag atgaatactt
      841 taagagctca gaaggaatga agatcatcaa ggatttatca catatttctt ttcttcatat
      901 ccctaaagag cagatcattg atgagatttc ttatatcatg ttgtttttga tcacggaaga
      961 agaatttgaa ttagatggcg catttggact tgcggaagct cattttcatg atatccacat
     1021 gattagtcaa aaaatgtcag tggaattgat tgatcaatta ccattttcga ttgaaatctc
     1081 tgaagaagaa agactatata tacgaaaaga acttcgtatg ggtatgatgc atgtcaatcg
     1141 aaaaaattgg atattcgact ttgagatgac tagctttacg tctaaaaagc agctacagtt
     1201 tttcttcgag acgtatccaa tattcagtac agcaatccaa aaggtcatac aagagaataa
     1261 acgctatttc ttgggaaaag atgaacgttt catgattcgc ttattttatg attatctctt
     1321 tttattggtc ggtatttgtc cggtatcata ttttgaagca ccgattcatg tctgtattga
     1381 tttttcgcaa ggatctgctt ataccgatta cattatttct caagtgaaag gatttaaaaa
     1441 ttacaatatt gtgattgaga gtcgaatcac ttctcgtacg aatatttatt tatctgattg
     1501 tttacttgaa aaatttaata aagaacagat aatttggaaa aatccaccga ctccagatga
     1561 ctgggaatat tttggaaata ccatcatcag agaaaaaaat agacatttaa ctgaaaaaat
     1621 cagctagtat ttatggatgg cagctttgtc tcacatattt attagctgat ttatgtt
//
LOCUS       DAFCLG010000205         1671 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFCLG010000205 DAFCLG010000000
VERSION     DAFCLG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.215"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..1518)
                     /locus_tag="LND56_002743"
     CDS             complement(52..1518)
                     /locus_tag="LND56_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBK6744605.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          568 a          338 c          300 g          465 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat t
//
LOCUS       DAFCLG010000206         1641 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFCLG010000206 DAFCLG010000000
VERSION     DAFCLG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.216"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="LND56_002744"
     tRNA            complement(21..94)
                     /locus_tag="LND56_002744"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(283..1302)
                     /locus_tag="LND56_002745"
     CDS             complement(283..1302)
                     /locus_tag="LND56_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK6744606.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1313..1519)
                     /locus_tag="LND56_002746"
     CDS             complement(1313..1519)
                     /locus_tag="LND56_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6744607.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          456 a          345 c          289 g          551 t
ORIGIN      
        1 gattcaaatg gctacgttaa tgtaccctgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctgaccaac tgagctaagg gtactggttg ttgccacata aagccataaa
      121 caatcaacca gtagaatgtg tggcaacaaa cctgttatcg catagcttgg agtgtgacta
      181 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaatga tttttatagt
      241 tgtcaatata gttatgtaat gctcctcaac gaggagctat ttttatcgtt taggaatatt
      301 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
      361 attcgttgcc cgcatcatga catagacttt cttattaggg aagttacgca tgttaaaaga
      421 tacatgatag ccaacatttc cagaagtatt ataagcttga tttacatctg atctataaat
      481 tccatcagct cttactcgag ctaattcttt cccagtattg taatccataa tgaagatata
      541 ctcgtattta tagttagcaa tgtgccatcc agctacatgc aaatttgcat tttcgatttc
      601 tccaaactga tcaatgtggg cgtaattcgt tccatctgtc agtgtaggat tagctgcacc
      661 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggtaaa
      721 tccatgagct aaatcatatg ctaatttttc tttacttacg cccatttcag aaagataacc
      781 gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc cataaatgcg atttgattcc
      841 cggttggtta taaggagtgt ccaacgttaa tggaatacca tatttcattg ctgaatctct
      901 agccaattca acgtatgcct tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg
      961 taactcaatc tgcacaggac tgttggcatt agcatacgaa ccagcaccgt actgtacata
     1021 accaggttga ccgacttgat aaacaattcc gccgtctccc acaatataag cagtataagc
     1081 gctagtccat gaacgttgca tatactgcgc ttcattgcgt cctgttgctg tttcattagc
     1141 cgtttcatgc agtaaaatat actgattatt tgctacttgt gagctacctt catttgcgcc
     1201 caaattaaat tcattgttga tagtataggc aaatccgtta attggcaata aaaaaagagc
     1261 cgttaacagg ctcatcgcag taatagtaat tttctttttc atatctatcc tcctatttat
     1321 ctgatgaaat tttcttatac gttcgatttg atacacctaa aatactccct agaaatgctc
     1381 caaaacctgt aataatcatc accgttatat ctgtgtaccg ccagttaaca ccttttccga
     1441 caagaccgac aaaagtcgct aaagcgggga taactactat agcgatccat ttcaaaatat
     1501 caaaggttct attgttcata ctatccacct cccttcattg gttgggctac tttctttttc
     1561 aatcgcttgt tttcttctat caatatttct ctttctgaaa gcaaggcatc aatttgcccg
     1621 tccagttgtt caattttatc t
//
LOCUS       DAFCLG010000207         1619 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFCLG010000207 DAFCLG010000000
VERSION     DAFCLG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.217"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            287..694
                     /locus_tag="LND56_002747"
     CDS             287..694
                     /locus_tag="LND56_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744608.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVNLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="LND56_002748"
     CDS             708..1109
                     /locus_tag="LND56_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744609.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="LND56_002749"
     CDS             1111..1482
                     /locus_tag="LND56_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744610.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          607 a          208 c          281 g          523 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taaacttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaa
//
LOCUS       DAFCLG010000208         1591 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFCLG010000208 DAFCLG010000000
VERSION     DAFCLG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.218"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            58..432
                     /locus_tag="LND56_002750"
     CDS             58..432
                     /locus_tag="LND56_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6744611.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(546..1175)
                     /locus_tag="LND56_002751"
     CDS             complement(546..1175)
                     /locus_tag="LND56_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6744612.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1309..1381)
                     /locus_tag="LND56_002752"
     tRNA            complement(1309..1381)
                     /locus_tag="LND56_002752"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:ggt)
BASE COUNT          560 a          230 c          272 g          529 t
ORIGIN      
        1 ttttattaat atatgctagc atgcttttgt aagaaaaaga aagagggcgt attagttatg
       61 tatcttaaaa ttacggaaca agcacaagaa agattacaaa aatatattga tgaaggcgca
      121 acagttatat tggatttaga tgatggcgtt ggggaatatt ctaaaatggg tgtttgctct
      181 ttagatacta gttttcgttt acttcttctt gataaagaac agagaaaaaa tgattataaa
      241 ctccaattag atagtgatat tggggatgtt tatataaaag attattcaaa aatgtacatg
      301 gatgaacata tgaccctttc tttagatcca cgtctaggtg tatttactct tgaaagccca
      361 agtggttctt tagacagtca tgttcaatta gtagatctac gaccagaaaa attctctgga
      421 ataaaacaat aactttctct ctattgtcca taaaactagt ctcaatcaag ctgtattgaa
      481 aaaacaagac aacattctta ccatggtaaa taaaaagacg acacattttg tcgtcttttt
      541 ttaaattaac tattttcgat aaaagatcgc atagcttgtg ccattatttc tggcttttct
      601 ctattcaatt catgttttcc gtttttaata aagagtagcc ggccattcgt tgaaagttta
      661 tataaacgtt ttgctgctgg tttattcgca tgatctttgg taccgcataa aatcaattta
      721 ggaagtcgtg atgtacgaag ctgttttttg cagtctaatg tttcaagtga acgaagtagg
      781 gtaagggttt gttttttaga tagacctaat ttttcaaata tcttagaagg tacgcagtaa
      841 aaataaatat tctggaaagt aagcaataag cgattaggag actcaaattg tggagcgact
      901 aaaacaacct tttttaaata tggagaagga gttgcaagat agtttaaagc aataacagcg
      961 cctaaagata atccacataa aataaaaggt tcctttatct gattcaattg tacttcgatg
     1021 tatttggtta agtcgtctaa agtagcagtg tctgtgctgt ttaaatgatt gaacaatgaa
     1081 agacttttgc aagaataact attgggtaga taagagatta catctttcca tgattgttcg
     1141 tcttggccta gaccatgcaa aaaaacaatt ttcatattgc tctccttttt atagttatca
     1201 tatagacttt ctttgtacta ttaaaatata aacattcatt tcttacgtca taaaataaaa
     1261 aaacagcgca gacacattgt taaaccaaca tgttgcactg tttaaggaag ccacttgccg
     1321 ggcttgaacc ggcgacctct tccttaccat ggaagcgctc taccagctga gctaaagcgg
     1381 cattatcatt aatacaaata ataggataac gaatattaat gtatttgtca acagaatttt
     1441 ttcgaaaaaa tgtgtttctc taataggaaa acagatttta gaaaaaataa aaataacaaa
     1501 aaatgcgaac ggtttctttg ataatataat aaaaaaattc aaaaaaagta ttgcgttatt
     1561 tagaagaaat tgatattata ttcatgtact t
//
LOCUS       DAFCLG010000209         1588 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFCLG010000209 DAFCLG010000000
VERSION     DAFCLG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.219"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(141..1433)
                     /locus_tag="LND56_002753"
     CDS             complement(141..1433)
                     /locus_tag="LND56_002753"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK6744613.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          448 a          338 c          277 g          525 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaaaaa gaggttgt
//
LOCUS       DAFCLG010000210         1572 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFCLG010000210 DAFCLG010000000
VERSION     DAFCLG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.220"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="LND56_002754"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="LND56_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HBK6744614.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            165..899
                     /locus_tag="LND56_002755"
     CDS             165..899
                     /locus_tag="LND56_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBK6744615.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          588 a          248 c          211 g          525 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacattcatt ataacctcat aggagtgggt tataatgaac aaaaaaaata
      181 taaaagatag tcaaaacttt attacttcaa agcatcatat aaatgaaatt ttgagaaatg
      241 tacatttaaa tacaaatgat aatattattg agattggttc agggaaaggt catttcacgt
      301 ttgaattagc taaaaggtgt aattatgtaa ccgccattga aatagatcct aaattatgta
      361 ggataactaa aaacaaactt attgaatatg agaacttcca ggttatcaat aaagatattt
      421 tacaatttaa gtttcctaaa aataagtcat ataagatata tggaaatata ccctacaata
      481 taagtacaga tataattcga aaaattgttt ttgaaagcac agctacagaa agttatttaa
      541 tagtggaata tggatttgct aaaaggttgc taaatacaaa tcgttcacta gcactatttt
      601 taatgacaga agttgatata tccatattaa gtaaaatccc tagagaatac tttcatccaa
      661 aacctagagt taatagctcg ttaattgtat taaaaagaca cccttcaaaa atatcactca
      721 aagatagaaa acaatatgaa aattttgtta tgaaatgggt taacaaagaa tacataaaac
      781 tattttccaa aaaccaattt tatcaagcct taaaatatgc aagaattgac gatttaaaca
      841 atattagctt tgaacaattc ttgtctcttt tcaatagcta taaattattt aatagataag
      901 ttaaggggtg cataaactgc atcccttaac ttatcttatt ttgcttaaac aatcttggcc
      961 cgtaaactgc ataactcata tgtaaatcgc tcctttttag gtggcacaaa tgtgaggcat
     1021 tttcactatt tccggcaatt acttccatgt aaagtataac ccactatact ttgcaagcaa
     1081 agtgtgggct cgccgagggg tcttgcagac ggctctttcg gctcttttga gccgagtatc
     1141 agcgcaccct tatggtgcaa ttagctgata cctgtttgac gatttgctgc cctacctttt
     1201 cggtaggtat gcaaacctca aaccaaaata aaaaccccct tcgacttcgt cagggggttt
     1261 ttatttatat aaatctccgt aaataatcgc gatttaataa catcttcaat tttatttttt
     1321 tgcgtatttt agaaatgttt tcactaaaaa atatattata caaaaaatac agtaatcgat
     1381 tataattctt tgccagttaa atatcatatc gttattaaaa tatcggaaaa aaatatcaac
     1441 aacaataaat gcaaaagaag accataaaaa ttgtttatta ctcatacata ctctccattt
     1501 ctatcatttt taaatttacc agtcatctct caaaaatctc ctagctattt ttctttttta
     1561 accacatacc gt
//
LOCUS       DAFCLG010000211         1556 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFCLG010000211 DAFCLG010000000
VERSION     DAFCLG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.222"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..330
                     /locus_tag="LND56_002756"
     CDS             <1..330
                     /locus_tag="LND56_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744616.1"
                     /translation="ELVKKIEGDLSYILENEIFVNKTRRLLLGRCRTCIQKAVRYASF
                     NKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQSIIGVKTLAYLSG
                     MGRNRRF"
     gene            648..1238
                     /locus_tag="LND56_002757"
     CDS             648..1238
                     /locus_tag="LND56_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBK6744617.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          567 a          173 c          301 g          515 t
ORIGIN      
        1 gaattagtta aaaaaattga aggcgattta agctatatat tagaaaatga gatatttgta
       61 aacaaaacaa ggagactttt gttgggacgc tgtcgtactt gtattcaaaa ggcagttaga
      121 tatgctagtt ttaataaaga atttgattta aactcctatt tgtctaagat acttatgaac
      181 gatgagttga ttgcctgtat tcactcatac ccatataaac aaaatccaat taaacaacga
      241 atatttaact attttttact aaaccaatcc attattgggg ttaaaacatt agcatattta
      301 agcggaatgg gtagaaatag gagattttaa tcataaaaaa ctgtactata gtgaattttt
      361 ggagatttat attccagtta tatattaatg attgtgatac ttgtactatt gtaaaaattc
      421 gttaggttta aataactgga aatatcggaa aatgggcatt atacatcacg gacatccctt
      481 cttttggaaa aaatgttacg atttttataa gtaatattgt atccggtgga atcaacgatg
      541 aagataacat atccatcgtt gtagattcag tagcattgaa tgatgctctt gttgcaagaa
      601 atccgaataa aattgttaag tataaattga attaatagga gatatgaatg gaaattagca
      661 gtaaagaata tttaaaagcg gttcttcgta tggaaaagaa tctatatata gaatctaaag
      721 gacaacacac aaaattacta aaaataatga aatcacatcc ggactacaac gcttggcggt
      781 atactcgtcg aatgagaatt acatcttatt attattcaaa gagaaagaaa aatatcattt
      841 atgcgattat gtatattgtg tcttgcagaa gaatgaatag tttgggaaga aaatgtggta
      901 tagagtgtgg tgaaaatgta tttgatgaag gtgtaagatt ttaccataca caaggaattg
      961 ttattaatgg taacgccata ataggaaaaa attgttactt atatgggaat aactgtattg
     1021 gaaatgatgg aatcgatcct aagtgtcctg taattggaaa taatgtacgt gtatgcgtgg
     1081 gtgcaaagat tattggtaat gtaaaaatag ctaacaatgt agttgttgcg gcaggagcta
     1141 ttgttatcaa agattgtcta gagaatggtg caattcttgc aggtgtaccg gcaaaaataa
     1201 tagggtatgt taatttaaca aaagtatttt tagaataatt tttagcgaaa gaaggtgaat
     1261 aacgatgcgt atttcaataa aaagatggtc aggagcagat tggacattgt tcacgttggc
     1321 attttttgca atctccatag ttcttgtata tacttttaac gccccaagag caattttata
     1381 tttgggagat ataattaata tctttgtgtt attaaatgca cttagcttta gacgcagaaa
     1441 aggaatatat gctagcaaaa atgagatgct gatagttttg tttttactag tttctatttg
     1501 tagctcagtt attaatttag aaaatatagg attgattatc tgggggatac gaaata
//
LOCUS       DAFCLG010000212         1536 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFCLG010000212 DAFCLG010000000
VERSION     DAFCLG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.223"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..400
                     /locus_tag="LND56_002758"
     CDS             2..400
                     /locus_tag="LND56_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6744618.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="LND56_002759"
     CDS             384..1079
                     /locus_tag="LND56_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK6744619.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          581 a          221 c          303 g          431 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaat
//
LOCUS       DAFCLG010000213         1488 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFCLG010000213 DAFCLG010000000
VERSION     DAFCLG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.224"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            225..1100
                     /locus_tag="LND56_002760"
                     /note="aph(2'')-Ia"
     CDS             225..1100
                     /locus_tag="LND56_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBK6744620.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1230..>1488)
                     /locus_tag="LND56_002761"
     CDS             complement(1230..>1488)
                     /locus_tag="LND56_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744621.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          596 a          139 c          251 g          502 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
      421 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
      481 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
      541 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
      601 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
      661 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
      721 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
      781 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
      841 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
      901 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
      961 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1021 tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata
     1081 aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa gacattagta
     1141 ttaaatagtt taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg
     1201 taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa
     1261 gaataaatcc attcatcatg taggtccata agaacggctc caattaagcg attggctgat
     1321 gtttgattgg ggaagatgcg aataatcttt tctcttctgc gtacttcttg attcagtcgt
     1381 tcaattagat tggtactctt tagtcgattg tgggaatttc cttgtacggt atattgaaag
     1441 gcgtcttcga atccatcatc caatgatgcg caagcttttg aatatttt
//
LOCUS       DAFCLG010000214         1466 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFCLG010000214 DAFCLG010000000
VERSION     DAFCLG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.225"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1014..1304)
                     /locus_tag="LND56_002762"
     CDS             complement(1014..1304)
                     /locus_tag="LND56_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744622.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          469 a          288 c          205 g          504 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tataga
//
LOCUS       DAFCLG010000215         1459 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFCLG010000215 DAFCLG010000000
VERSION     DAFCLG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.226"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..484
                     /gene="spx"
                     /locus_tag="LND56_002763"
     CDS             74..484
                     /gene="spx"
                     /locus_tag="LND56_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6744623.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            498..854
                     /locus_tag="LND56_002764"
     CDS             498..854
                     /locus_tag="LND56_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK6744624.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          538 a          211 c          271 g          439 t
ORIGIN      
        1 tttattagct aataattggt attttaaaag aaattactgt agaattggat tataattaaa
       61 aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct gtagaaaagc
      121 aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt ttgccgaacc
      181 gttaacagaa agtgagatca aaaagatctt ggcagcgact gaaggaggag ttcaagatat
      241 tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg aattaacttt
      301 aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac ctattttgat
      361 tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat ttgtccctcg
      421 tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca tcttaaattc
      481 atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt gtaacaagtc
      541 tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt gacttagaag
      601 aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa gtgcaagcag
      661 aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac ctttcctgtg
      721 taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc agacgtcctt
      781 tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg gaacagctta
      841 tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca tacagttgta
      901 acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt atttttctac
      961 tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga tatagagtta
     1021 aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt ttaagtagca
     1081 aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt tagaaatgac
     1141 atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa
     1201 aaacttcaaa aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt
     1261 aagatttcga aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc
     1321 tctgatatga tataaaagtt gctacggcag ccaaaaacga atcggggaac actcctgatg
     1381 aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt
     1441 caattcaaga caacaaaca
//
LOCUS       DAFCLG010000216         1424 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFCLG010000216 DAFCLG010000000
VERSION     DAFCLG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.227"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="LND56_002765"
     CDS             complement(150..1337)
                     /locus_tag="LND56_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBK6744625.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFCLG010000217         1415 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFCLG010000217 DAFCLG010000000
VERSION     DAFCLG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.228"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..110)
                     /locus_tag="LND56_002766"
     tRNA            complement(38..110)
                     /locus_tag="LND56_002766"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Gln,seq:ctg)
     gene            complement(156..584)
                     /locus_tag="LND56_002767"
     CDS             complement(156..584)
                     /locus_tag="LND56_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744626.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(644..1402)
                     /locus_tag="LND56_002768"
     CDS             complement(644..1402)
                     /locus_tag="LND56_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK6744627.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          403 a          271 c          270 g          471 t
ORIGIN      
        1 tttacagggg gttcattttt tcagcaagca catcaaattg ggatggcagg gttcgaacct
       61 acaattgctt gattcagagt caagtgcctt accagtttgg ctacatccca tttattcaac
      121 aaaataatca tagcactttc ctaaaaaaac gcaagttatt tttcttgatc ggatagttgt
      181 tgctttactc gtaaaaggcg gcgtactagt ttcttttcta atccagcagg aagggcaatc
      241 agttctgcta ctgacaagac ggtgtccaat aactgatatt ctttgacagg ttcattcata
      301 atatgataga tgtttgctaa acgttcaata tattcaaagc gagtatactc tgttcgagca
      361 attagaaatt tcaataaatc ggtggctttc tcgtactctt gatctgcgta ataaacaaga
      421 gattgacgat acaatttgat ttccagttca ttgagggtag caggtctgcg acgctcgttc
      481 cactcattaa tagagacttt accttgaaca tttcccttgc agtcacggac aaaaataatc
      541 ggaatttcat ttggatccat ttttttatgt gtgaattttt ccattatcta cgctcccttc
      601 aaaggtttgg tatcttgagt ataagggatt tttgtttgaa ttactagctt ttctccaaag
      661 ttagcatctg tccacccatt gcagtgaaaa tcgccgaaaa atcgttgatc ggtactgctc
      721 gattttttac accataggga tcgttgacat aaatattttc tttgtcatat ccagtgacta
      781 cacaagcatg aacaagagga gtgactctta cttctccatt atttgtggtc caagtcataa
      841 aatcagtttc atctgggact tcaaaattga tagtcacaga agtccagaca ggatttcctt
      901 tagatagtgc ttttttgata tcatcgagtt gagcatcggt ttttggaaaa gtgtgataat
      961 ccgtttggac gatttgctga gcaagggagg caactggggg aacatagacg accatagccc
     1021 agtctccgcc actgatatca ccaacaaatc cttcattagg atcgccatga gtcccatctg
     1081 cattgaaaac agggacgtaa tccaattgct cagctaactg gtttttagtt gtttcataac
     1141 cataaaaaga taaaagcata gaaagcgaag tgacttcaca gccattttct aaagaaggag
     1201 aatcaaactg gctctctaat ggaacatcta gtaaaacttc tgctttctct tttgtctttt
     1261 tcgttttttg tgttgaactt ttttgtgcag tgttcattgt atgttctgga gaatggatat
     1321 gtaggtaata acttgtaccg cttaataaaa gagcagctgt gccccctaga aacaatagtt
     1381 ttttgaacgt ttttttcttc atttgaaatc tccct
//
LOCUS       DAFCLG010000218         1405 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFCLG010000218 DAFCLG010000000
VERSION     DAFCLG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.229"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..755
                     /locus_tag="LND56_002769"
     CDS             <1..755
                     /locus_tag="LND56_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6744628.1"
                     /translation="NKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTT
                     ASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDGY
                     YNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDP
                     NSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVV
                     DAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(870..1310)
                     /gene="fabZ"
                     /locus_tag="LND56_002770"
     CDS             complement(870..1310)
                     /gene="fabZ"
                     /locus_tag="LND56_002770"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6744629.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          463 a          273 c          256 g          413 t
ORIGIN      
        1 aaaataaagt tattttaagt attagtttag cagcagcatt gctgtttgct gctggttgtt
       61 ctgcaaacaa cacgacttct tctagttcgt ctacagaaag ttcaaaaagt acatctactg
      121 cggaaagcac gactgccagt agcgtcgatt tgaacgcatt ggaattacca caattgacga
      181 aagatgtcgc cgaaaatgaa gatctggtcg aaatggtgac gaataaaggc acaatcaaaa
      241 tcaaattatt tcccgaacac gcaccaaaag cggttgaaaa ctttatgaca catgcaaaag
      301 acggttacta caacggactg actttccatc gcgtcatcaa agatttcatg atccaaggtg
      361 gcgatcctaa aggcgatggc actggagggg aaagcatttg gggagaagga tttgaaacag
      421 agatcaacaa ccacctttat aatataagag gtgcgttatc tatggctcgc tcacaagatc
      481 ctaacagtaa cggcagccag ttctttatcg ttcaaaatac agatgatatg catgatggtc
      541 ttttaaaaga cgactatcct caagcaatca tcgacgctta taaaaacggc ggttatcctt
      601 cactagatgg ccattatact gtcttcggcc aagtgattga aggaatggat gtcgtggatg
      661 ctattgcaaa tatcgaaaca gatagttcag acaaaccaaa agaagaagta aaaatcgata
      721 aaatcaatat actgcaagaa gcaaaacaaa aataaaaaaa taacgtctga atcctgcata
      781 gatgacaacg tatgctagga ttcaggcgtc ttctttttac ataaaaagca agtaagcttc
      841 cttttgtttt gctatatcag gttttatagt caagcatttt ggttgacgat gaacgtgaac
      901 tcgcactcac agactttttt atcatctaca tatgctgcag ataacgctgt accgactgat
      961 cccttcattt tggtgatttc aaaatgacat ttcagcacat ctcctggaac aactttttga
     1021 cggaatttgg ccttattgat cccaccgata taagctcttt ttcctagaaa ctcttctgat
     1081 tttaggataa gtatcgaacc tgcttgtgcc agtgtttcta gaatcaacgc acccggcatc
     1141 actggattgt caggaaagtg accttggaaa aaatcttcat tgattgttac attttttgtc
     1201 gcaacgatct tttttcctgg ttctaaagta tctacataat caatataaca aataggataa
     1261 cgattaggaa tcaattccat tacttcagta gcagataata cgtaagacat aattaaactc
     1321 cttctttcag gcaattgcca ttttcattac tttgactatc aaagtatcat attcctaaat
     1381 atttagcaat ctttttttaa tttaa
//
LOCUS       DAFCLG010000219         1356 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFCLG010000219 DAFCLG010000000
VERSION     DAFCLG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.230"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1342
                     /locus_tag="LND56_002771"
     rRNA            <1..1342
                     /locus_tag="LND56_002771"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          369 a          295 c          409 g          283 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaa
//
LOCUS       DAFCLG010000220         1349 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFCLG010000220 DAFCLG010000000
VERSION     DAFCLG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.231"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..697
                     /locus_tag="LND56_002772"
     CDS             74..697
                     /locus_tag="LND56_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744630.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            complement(694..>1349)
                     /locus_tag="LND56_002773"
     CDS             complement(694..>1349)
                     /locus_tag="LND56_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744631.1"
                     /translation="FLEELSGEEYRLFLNNNILKLISYAKKANSLSLVDNLDNLLPDK
                     LIYDENEDNSQKQIIIFELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPFF
                     TLINFMLYVKEHLGYDDLVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYKE
                     QNIKTLELIKKNIKYYDPLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
BASE COUNT          483 a          205 c          201 g          460 t
ORIGIN      
        1 ctttatagtg tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa
       61 gtaggtaaaa attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca
      121 acgccaaatt gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc
      181 tggagcaacg attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca
      241 agatattttt attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc
      301 ttcagtcaat tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat
      361 ggctgaagca attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat
      421 tttggccatg attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat
      481 taaaattgca aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac
      541 aaaaaatcct caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat
      601 agctatttca aaaattgcta aaaactacaa tattactaga caaactattt acagaattaa
      661 gaatgatatt gagtataaca gtaatcaaat gaattaaaaa agttctttat acattaaaat
      721 ttcttctttt aactcatcaa ttctaggaga tctaacatca gaatcagcaa ttgcatttaa
      781 taacttatca atattttctt tattcgctag taaaggatca tagtatttta tattcttttt
      841 aattaattcc agagttttta tattttgttc tttgtaatta gctagaattt cttctacagt
      901 ttcgcctgaa atcatagcca tatctgaacc agtattgatt cctttgaatg gataaggttc
      961 tttatcatat gcctttatca tttctactaa atcatcatat cctaagtgtt ctttaacata
     1021 cagcataaaa tttattaatg taaaaaatgg cattgaagaa tagtctggaa atataaattg
     1081 aaacagtttg ttcgcaaaat atttatcatc atcattgctt tctggtgaca ctgacaggct
     1141 gctaagccac tttaaataca attcaaatat aattatttgt ttttgtgaat tatcttcatt
     1201 ctcatcataa ataagtttat cgggtaaaag attatctaag ttatctacta aagataggct
     1261 attagctttc ttggcataag atattaattt taaaatatta ttatttagga ataatctata
     1321 ttcttctcca gataattctt ctaaaaatt
//
LOCUS       DAFCLG010000221         1344 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFCLG010000221 DAFCLG010000000
VERSION     DAFCLG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.232"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1344
                     /locus_tag="LND56_002774"
     CDS             82..>1344
                     /locus_tag="LND56_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBK6744632.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRI"
BASE COUNT          458 a          260 c          256 g          370 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatc
//
LOCUS       DAFCLG010000222         1334 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFCLG010000222 DAFCLG010000000
VERSION     DAFCLG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.233"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            86..1165
                     /locus_tag="LND56_002775"
     CDS             86..1165
                     /locus_tag="LND56_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6744633.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DAFCLG010000223         1300 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFCLG010000223 DAFCLG010000000
VERSION     DAFCLG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.234"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..1168)
                     /locus_tag="LND56_002776"
     CDS             complement(74..1168)
                     /locus_tag="LND56_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6744634.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          387 a          270 c          200 g          443 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt
//
LOCUS       DAFCLG010000224         1296 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFCLG010000224 DAFCLG010000000
VERSION     DAFCLG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.235"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..>1296
                     /locus_tag="LND56_002777"
     CDS             46..>1296
                     /locus_tag="LND56_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6744635.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          401 a          258 c          251 g          386 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct
      361 gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag
      421 aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt
      481 gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta
      541 tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg
      601 gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa
      661 cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat
      721 atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc
      781 gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa
      841 accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag
      901 cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg
      961 cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct
     1021 tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa
     1081 agagatcagt cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc
     1141 tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac
     1201 tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc
     1261 aatgcgtacg gctttcgcaa cttttacaat ttcaaa
//
LOCUS       DAFCLG010000225         1262 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFCLG010000225 DAFCLG010000000
VERSION     DAFCLG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.236"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            335..1246
                     /locus_tag="LND56_002778"
     CDS             335..1246
                     /locus_tag="LND56_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6744636.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          499 a          191 c          253 g          319 t
ORIGIN      
        1 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
       61 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
      121 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
      181 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
      241 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
      301 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
      361 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
      421 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
      481 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
      541 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
      601 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
      661 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
      721 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
      781 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
      841 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
      901 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
      961 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     1021 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     1081 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     1141 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     1201 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
     1261 aa
//
LOCUS       DAFCLG010000226         1255 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFCLG010000226 DAFCLG010000000
VERSION     DAFCLG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.237"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..1064
                     /locus_tag="LND56_002779"
     CDS             174..1064
                     /locus_tag="LND56_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBK6744637.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          448 a          225 c          268 g          314 t
ORIGIN      
        1 atatgagctg cgtaaaatgc atgtttctca tatgcttttt ttattattat gtaaagaatg
       61 cgaaaaacgt ttacatgatt tttacataac ttaactatat ttttacagca accatacgag
      121 ggatttacag tcgactgata aactttagtt gtaaataaga aaggagcaat aaaatgaaaa
      181 aagtattgtt tactctaggt atgatgggaa ttcttttagc cggatgcggc tcaggatcag
      241 gaaatacaga tggctctgcc acacaaaata attcaagtga ctcaaatgaa caagtaaaaa
      301 tcgttgcggt aggttcgaca gcattacagc cgttagtcga tgcagcacaa gaaagctttg
      361 tacaagaaaa tccaaattat caaatctctg tacaaggagg aggaagcgga actggtttga
      421 gccaagtcga agctggagca gttactatcg gaaactctga tgtatttgca gaagaaagag
      481 acggagttga tgcttcaaaa ttagtggatc ataaagtagc agtggtcggg atggcaccaa
      541 tcgtcaataa agatacagat gtcaaagata tcaccaaaca ggaattgatt gatatcttta
      601 caggaaaaat cactaactgg aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga
      661 accgtgcaaa cggcagcggt acccgcgcga cattcgaaaa atggggactg gatggtgcta
      721 cccctgtgca gtcccaagaa caagattcat caggaacagt tcggcagctg gtaagtcaaa
      781 caccaggagc aatcagctat ttagctttct catatctcga tgattccaca caagcactaa
      841 gtatcgacgg tgtagaacca aaagaagaga atgtagcaga taacagctgg ggaatctggt
      901 catatgaaca tatgtacacg aacggaaaac cttcccctga agtccaaaaa ttcttagact
      961 acatgatgac agaagaaatc caagaaggac ctgtcaaaga attgggctat ttaccaatca
     1021 caatgatgga agtagaacgt gatcacgaag gaaatatcaa ataactagca gataaacatg
     1081 ccacggtagt cgttactatt aaggaaaaat caactatgtt tccccacata tacatttttc
     1141 ctttaattgt gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     1201 agctgattga acaccgttta ttcgaaaaaa agagctacga catagaactg tcgta
//
LOCUS       DAFCLG010000227         1248 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFCLG010000227 DAFCLG010000000
VERSION     DAFCLG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.238"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..535)
                     /locus_tag="LND56_002780"
     CDS             complement(170..535)
                     /locus_tag="LND56_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744638.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            743..1225
                     /locus_tag="LND56_002781"
     CDS             743..1225
                     /locus_tag="LND56_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744639.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          419 a          177 c          230 g          422 t
ORIGIN      
        1 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
       61 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatataaga aaaacatata
      121 tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt tatggatgcc
      181 gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt ggaactaagt
      241 aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag aagtaacgcc
      301 attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata ttggcaatga
      361 ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga gataaaaata
      421 tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag gcgaaatgca
      481 cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt atcatctttc
      541 atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa tcctgcaata
      601 atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc attatctcta
      661 aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat gatgagagaa
      721 aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc tttatttatt
      781 tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg
      841 tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga attttccagc
      901 tttgattgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat ctctgatcaa
      961 tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat ctatgttttc
     1021 tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat tgaaggattt
     1081 agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag taattgcctc
     1141 atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa gtgaacgatc
     1201 tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaaac
//
LOCUS       DAFCLG010000228         1246 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFCLG010000228 DAFCLG010000000
VERSION     DAFCLG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.239"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="LND56_002782"
     CDS             200..742
                     /locus_tag="LND56_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744640.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="LND56_002783"
     CDS             898..>1246
                     /locus_tag="LND56_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBK6744641.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAFCLG010000229         1233 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFCLG010000229 DAFCLG010000000
VERSION     DAFCLG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.240"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            60..>1233
                     /locus_tag="LND56_002784"
     rRNA            60..>1233
                     /locus_tag="LND56_002784"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          379 a          246 c          351 g          257 t
ORIGIN      
        1 ttcttgatct aacttctatc gctagaagaa ggatcaaaac ccaaccgtaa ggttgataag
       61 gttaagtgaa taagggcgca cggtggatgc cttggcacta ggagccgatg aaggacggga
      121 ctaacaccga tatgctttgg ggagctgtac gtaagctatg atccagagat ttccgaatgg
      181 gggaacccaa catctttaat aggatgttac gattgtgtga atacatagca cattcgaggt
      241 agacgcagag aactgaaaca tctaagtacc tgcaggaaga gaaagaaaat tcgattccct
      301 gagtagcggc gagcgaaacg ggaaaagccc aaaccagcaa gcttgcttgt tggggttgta
      361 ggactccaat atggtagttc tttcagatag tcgaatgact tggaaaagtc agtcaaagag
      421 ggtaaaaacc ccgtagacga aatgtggaag acacctagga ggatcctgag tacggcggaa
      481 cacgagaaat tccgtcggaa tccgggagga ccatctccca aggctaaata ctccctagtg
      541 accgatagtg aaccagtacc gtgagggaaa ggtgaaaagc accccggaag gggagtgaaa
      601 tagaacctga aaccgtgtgc ctacaacaag tcaaagcccg ttaatgggtg atggcgtgcc
      661 ttttgtagaa tgaaccggcg agttacgatt gcatgcgagg ttaagttgaa gagacggagc
      721 cgcagcgaaa gcgagtctga atagggcgtt tgagtatgta gtcgtagacc cgaaaccatg
      781 tgatctaccc atgtccaggt tgaaggtgcg gtaaaacgca ctggaggacc gaacccacgt
      841 acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa attccaaacg aacttggaga
      901 tagctggttc tctccgaaat agctttaggg ctagcctcgg aattgagaat gatggaggta
      961 gagcactgtt tggactaggg gcccatctcg ggttaccgaa ttcagataaa ctccgaatgc
     1021 cattcattca tatccgggag tcagactgtg agtgataaga tccatagtcg aaagggaaac
     1081 agcccagacc accagctaag gtcccaaaat atatgttaag tggaaaagga tgtggggttg
     1141 cacagacaac taggatgttg gcttagaagc agccaccatt taaagagtgc gtaatagctc
     1201 actagtcgag tgaccctgcg ccgaaaatgt acc
//
LOCUS       DAFCLG010000230         1215 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFCLG010000230 DAFCLG010000000
VERSION     DAFCLG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.241"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="LND56_002785"
     CDS             414..947
                     /locus_tag="LND56_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK6744642.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          390 a          225 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaca
//
LOCUS       DAFCLG010000231         1133 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFCLG010000231 DAFCLG010000000
VERSION     DAFCLG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.242"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..537
                     /locus_tag="LND56_002786"
     CDS             <1..537
                     /locus_tag="LND56_002786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744643.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..>1133
                     /locus_tag="LND56_002787"
     CDS             564..>1133
                     /locus_tag="LND56_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744644.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHS"
BASE COUNT          479 a          179 c          252 g          223 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tct
//
LOCUS       DAFCLG010000232         1116 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFCLG010000232 DAFCLG010000000
VERSION     DAFCLG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.243"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..269
                     /locus_tag="LND56_002788"
     CDS             12..269
                     /locus_tag="LND56_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744645.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(501..932)
                     /locus_tag="LND56_002789"
     CDS             complement(501..932)
                     /locus_tag="LND56_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBK6744646.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          433 a          132 c          144 g          407 t
ORIGIN      
        1 cgtgaagata aatgtcaaat ttagaaaaca aagaagaaaa agttgtaaac aaaattgttt
       61 ctgttgtcaa taaactggat aaagagttag acgaacttga tacgttatct gaaaatccag
      121 aaaaaaaaca taacctaaaa aaatggttag ttgaacgaaa ggctattcat gaaattaaaa
      181 aagttcttca tgaagctgat aaatatgaaa aatatgatga aaaagaactt gataaagaat
      241 ttaaagaaat caatgactta ttactataat tgatttatac gactcatttt tgagtcgtca
      301 gactgcaaac aacactctaa atttaggagt ttgtctacag tctgaataaa ttttaaattt
      361 attgtccata atgttattat gaatttatag cattaagaaa agagataaga ctttacattt
      421 ttttaggaat gtaaagtctt atctcttttc ttaattgtac aattagtatt atctgcacta
      481 ctttctaatt aaataaattt ttagttatta acttgattaa aatcagagat aatatcattt
      541 aattcagttt gaagggttgt aacgattaaa tggaaatttt cttcatctgt tttattatat
      601 ggggatgttt tcgttatata taacaacttt tctttttcaa ttttcattaa taactctgtc
      661 gaagtctgta ctttatcttg ataattaaat gcagacatac cactttgtat aattgtaatt
      721 attgctgaag caattgaaac aatcaatagt aaagacctgt gttcggaagc ctgatcaatt
      781 aatattggga tactagctga taagactatt tttataatat ttccgatacc aattactcta
      841 ttataaaatc gaatttcttt ttttaatcta tcgattgtag aatcaataga ttcgataaac
      901 gctttttgat ccatcatttt ctctccaatc attttattaa aatcattaac aaaaatatta
      961 taataggatt taagtaagtt ctaactaatc acatataaaa ataaaaaaac atttgtattt
     1021 tatcaaagtt ttattatact tgtcaatttg tgtacatcct aaagagagta taaaatattt
     1081 tgtgtaaatg aaaaaatcca tacaaaaaag gaagtc
//
LOCUS       DAFCLG010000233         1061 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFCLG010000233 DAFCLG010000000
VERSION     DAFCLG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.244"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..995)
                     /locus_tag="LND56_002790"
     CDS             complement(39..995)
                     /locus_tag="LND56_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744647.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg t
//
LOCUS       DAFCLG010000234         1035 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFCLG010000234 DAFCLG010000000
VERSION     DAFCLG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.246"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="LND56_002791"
     rRNA            complement(<1..>1035)
                     /locus_tag="LND56_002791"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAFCLG010000235         1019 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFCLG010000235 DAFCLG010000000
VERSION     DAFCLG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.247"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            281..973
                     /locus_tag="LND56_002792"
     CDS             281..973
                     /locus_tag="LND56_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6744648.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          439 a          144 c          170 g          266 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaat
//
LOCUS       DAFCLG010000236         1009 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFCLG010000236 DAFCLG010000000
VERSION     DAFCLG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.248"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="LND56_002793"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="LND56_002793"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK6744649.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          264 a          221 c          158 g          366 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtat
//
LOCUS       DAFCLG010000237          948 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFCLG010000237 DAFCLG010000000
VERSION     DAFCLG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.250"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..>948
                     /locus_tag="LND56_002794"
     CDS             73..>948
                     /locus_tag="LND56_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6744650.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNI"
BASE COUNT          332 a          164 c          175 g          277 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatatt
//
LOCUS       DAFCLG010000238          936 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFCLG010000238 DAFCLG010000000
VERSION     DAFCLG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.251"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..370)
                     /locus_tag="LND56_002795"
     CDS             complement(<1..370)
                     /locus_tag="LND56_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744651.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVAL"
     gene            complement(387..791)
                     /gene="tnpA"
                     /locus_tag="LND56_002796"
     CDS             complement(387..791)
                     /gene="tnpA"
                     /locus_tag="LND56_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBK6744652.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          283 a          163 c          155 g          335 t
ORIGIN      
        1 tcaaagcaac tgtaccattt tgattattcg ttgtataact ctgaacagga tttttcttac
       61 ttttgaaacg tggaaatcca acggatttat cccgaaaaaa attcttgtat gctttatcta
      121 aattaagttg ggcattggct aaagcaaggc tatcaatttc tttcaaaaac ggaaattctt
      181 tcttgtattt agctggtgtc ggaaaagtca tttttttaga agaatcattt ttaacttctt
      241 catacgcctt ttttcggtca tcaagcatta gattgtagac cttacggaca caaccaaaag
      301 atttggcaaa gaagatttct tgttcctctg ttggatagat tctgaatttg tatgctttta
      361 gttgttccat aaagttcacc tactttctat ttatgattgt ttccttgatt ttggatatac
      421 ttcttaatca catcaattgg tgcgccacca gttgtcagaa gacaaaaact tttagaccag
      481 aacatttctt tccaaagaaa ttgtttgact cttggaaagt cacgttttat caatcttgag
      541 ctggcacttt tataggcatt gatgaatttt gtcaattctg ttttaggttg agctttgaac
      601 ataatatgaa cgtggtcttt atcatgattc cactccacta aagtaatgtg atacgattct
      661 gaaattcttt caaaagtagt tttggcataa tcagatattt catcatcaat cacttgtcta
      721 cgatatttcg ttactaaaac aaggtggtaa taaagaagaa atactgaatg attatttgta
      781 tctaattcca tgttatatca actcatttct tgtatagact gattgtaaca tggaacaaaa
      841 taaaaaggca atacgcctta gttgtcggtc ttcgagttac caacggtaac cctcataccg
      901 aacggcattc atcacccacc tatagaggat gggcga
//
LOCUS       DAFCLG010000239          910 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFCLG010000239 DAFCLG010000000
VERSION     DAFCLG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.252"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..355
                     /locus_tag="LND56_002797"
     CDS             <1..355
                     /locus_tag="LND56_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744653.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="LND56_002798"
                     /pseudo
     CDS             615..910
                     /locus_tag="LND56_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAFCLG010000240          908 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFCLG010000240 DAFCLG010000000
VERSION     DAFCLG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.253"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..>908
                     /locus_tag="LND56_002799"
     CDS             108..>908
                     /locus_tag="LND56_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6744654.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNY"
BASE COUNT          346 a          113 c          164 g          285 t
ORIGIN      
        1 tttcgatatg ttatttttta tgtttattaa cttcggaaga ctaaaattgt tactggtaat
       61 gatacatttg taaaaataaa aaagatgatt tttaggaagt gaagagagtg cataaaatta
      121 gtgttatagt acccgcatat aacgtagaag actatttaga aaactgctta gaatctattt
      181 ccaaccagac acattcaaat attgaaataa tagtagtcaa tgatggttcc ccggatcgaa
      241 gtggggaaat tgcagaagaa tataaaaaga aagatatgcg attaaaagtg atacatcaag
      301 tgaatggtgg tttaagtgcg gctaggaatt ctggtcttga ttctgcaaca ggagattttg
      361 tgtgctttat tgacagcgat gattggatag aaccagacta tcttgagtta ctatattttg
      421 gcacgcaaac ttatggagca gatatctcgg tgctaaaaat aaaaaagatt tcaagtttag
      481 aacaactaaa agaacataca attgctgaga gaaaatggga aatatttgat agagaacaag
      541 caatgaaaaa cttatttata aataataaaa ttggctattc tgcaaataat aaattattca
      601 atagaaattt atttgataat ataagattcc cactgggtaa attaatggaa gataaggcta
      661 ctacttattt gctgatcgat cattccgaaa aagttgctgt caattcatct gctaaatatc
      721 actattacca aagccctaaa agtatactga gaggaagctt caatcctaga atttttgatt
      781 cttttgaagt ccatgaagat atactaatat tcatagataa gttttatccg gaattatcaa
      841 ttaatgttag aactcgctat gtctatgaag caataaggat gatgatgaca atgattaaaa
      901 ataactat
//
LOCUS       DAFCLG010000241          901 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFCLG010000241 DAFCLG010000000
VERSION     DAFCLG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.254"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            109..>901
                     /locus_tag="LND56_002800"
     CDS             109..>901
                     /locus_tag="LND56_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK6744655.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKI"
BASE COUNT          391 a           98 c          146 g          266 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 a
//
LOCUS       DAFCLG010000242          898 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFCLG010000242 DAFCLG010000000
VERSION     DAFCLG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.255"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..>898
                     /gene="bsh"
                     /locus_tag="LND56_002801"
     CDS             4..>898
                     /gene="bsh"
                     /locus_tag="LND56_002801"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK6744656.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAID"
BASE COUNT          305 a          121 c          163 g          309 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaaactaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgata
//
LOCUS       DAFCLG010000243          890 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFCLG010000243 DAFCLG010000000
VERSION     DAFCLG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.256"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..888
                     /locus_tag="LND56_002802"
     CDS             <1..888
                     /locus_tag="LND56_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBK6744657.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAFCLG010000244          839 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFCLG010000244 DAFCLG010000000
VERSION     DAFCLG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.257"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..>839
                     /locus_tag="LND56_002803"
     CDS             2..>839
                     /locus_tag="LND56_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6744658.1"
                     /translation="MEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDYEKYDR
                     NGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLETTIIQL
                     FQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVIFMDAT
                     HIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRGLEEVL
                     LVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLVYQANS
                     KEE"
BASE COUNT          292 a          132 c          167 g          248 t
ORIGIN      
        1 tatagaaatt atgcaaacac tactcaataa aggggattta gatgaattat ttcaggatca
       61 tttagagcga gcgattaatt cacttctaca agcagagcta actgcttttt tagattacga
      121 aaaatatgat cgaaatggat tcaattcagg aaattcccgt aacgggaatt attcacgtac
      181 atttaaaaca gaatatggcg agttgaattt aactattcct agagatcgga atggcgaatt
      241 ttctcaacaa acactaccag catataagcg aaccaatgat tcattagaaa cgaccattat
      301 ccaattattt caaaaaggca ttactatggc tgaaatctcc aaattaattg aaaaaatgta
      361 tggtcaccac tatacgccac aaacaatctc caacatgagt aaattggtgg ctgaagatgt
      421 tttagctttt aaagaaagaa cgttagaagc caattattcc gttatattta tggatgcgac
      481 gcatatccct gtgaagcgac agactgtttc gaaggaagca gtgtatatta cgattggtat
      541 tcgtttggat ggaaccaaag aagttcttgg gtttactatt gccccaactg agtctgctta
      601 tatttggaaa gaagttcttc aagatcttag aaaacgtggg ttagaagaag ttttattagt
      661 agtgacagac ggattaagct gtattgaaga aagtatccat agtgtgtatc cgaatgccca
      721 atttcaacaa tgttgtgtgc atgtatctag aaatatcgct cataaagttc gtgttcgagg
      781 tcgaaaagaa atttgtgagg atttcaaatt ggtttaccaa gcgaattcaa aagaagagg
//
LOCUS       DAFCLG010000245          824 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFCLG010000245 DAFCLG010000000
VERSION     DAFCLG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.258"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            136..309
                     /locus_tag="LND56_002804"
     CDS             136..309
                     /locus_tag="LND56_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744659.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          276 a          134 c          104 g          310 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actc
//
LOCUS       DAFCLG010000246          818 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFCLG010000246 DAFCLG010000000
VERSION     DAFCLG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.259"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            647..>818
                     /locus_tag="LND56_002805"
     CDS             647..>818
                     /locus_tag="LND56_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK6744660.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVL"
BASE COUNT          285 a          103 c          117 g          313 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaa
//
LOCUS       DAFCLG010000247          811 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFCLG010000247 DAFCLG010000000
VERSION     DAFCLG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.260"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..672
                     /locus_tag="LND56_002806"
     CDS             <1..672
                     /locus_tag="LND56_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744661.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAFCLG010000248          801 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFCLG010000248 DAFCLG010000000
VERSION     DAFCLG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.261"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..752
                     /locus_tag="LND56_002807"
     CDS             168..752
                     /locus_tag="LND56_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744662.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          257 a          118 c          167 g          259 t
ORIGIN      
        1 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
       61 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
      121 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
      181 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
      241 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
      301 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
      361 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
      421 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
      481 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
      541 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
      601 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
      661 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
      721 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
      781 gccatttatc tagaaaagtg a
//
LOCUS       DAFCLG010000249          798 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFCLG010000249 DAFCLG010000000
VERSION     DAFCLG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.262"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          292 a          121 c          125 g          260 t
ORIGIN      
        1 gttagtaaat attagaggta aacaaactat gcgtacttgt taccttatga gttggaatgc
       61 tagtagtaga acagccaaat attattacta catttagtaa aaacaaaaca ttctttctat
      121 acaataaaat agcgtataga gaggatgttt ttaatttgaa aaggagtggt tatctttatg
      181 aaaaatagat catttgctat attagtatta tccgtaattt tattagatat attgttaata
      241 acattagctt taaaaacttt aaatttagta acaagttttg tcattatacc agtaattata
      301 ggaatgtcta tttatttcat caaaaaaaat aaacaatttt tttgaatgaa attacgggcg
      361 aaaataatca ctcaaaatta ttaataaaaa cgaatcaact actttttctg aagaaacata
      421 caaccgtaaa caacaattgt tgtttacggt tgtaaattat caaactatat ggtgacttgt
      481 tgcttaaaat aggttgaaat ttcgtttatt ttttttgaga aattttaccc acgtactacc
      541 catgtaccta tccacgtatt acccatgtac ccccccatga caaatgggcg gaggggcttg
      601 cggcgcttgg tattaaaggc attatccgcc tcccaggttt ctgaccattt tctctcataa
      661 gtggggtttt taccgcgttg agagaaaatg gtcagaaacc taaacgatcg gcaaagaaaa
      721 cataccaagc gccacaagca aaaaaagtta gagtaacttc cagaaaagaa aaatttttag
      781 aacaactttt gaaaattt
//
LOCUS       DAFCLG010000250          791 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFCLG010000250 DAFCLG010000000
VERSION     DAFCLG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.263"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..782
                     /locus_tag="LND56_002808"
     CDS             <1..782
                     /locus_tag="LND56_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6744663.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DAFCLG010000251          764 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFCLG010000251 DAFCLG010000000
VERSION     DAFCLG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.264"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>764
                     /locus_tag="LND56_002809"
     CDS             <1..>764
                     /locus_tag="LND56_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6744664.1"
                     /translation="KLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSD
                     FSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFV
                     EKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIG
                     TSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSS
                     FMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPK"
BASE COUNT          294 a           69 c          127 g          274 t
ORIGIN      
        1 aaaattaggt gatgaagttg atcttgaatt actagatatt gaaaaaagtg gaaaaataaa
       61 aagtcataaa tttaaaatta tagggatctt ttctggtaaa aaacaggaaa catatacagg
      121 attatcttct gattttagtg aaaatatggt gtttgtagat tattcaacaa gccaagagat
      181 attaaataat tcagaaaata atgaaattgc aaataaaatt ttaatgtatt ctggtagtgc
      241 agaatctaca gacttagcct taaacaaatt gaaagagctt aaaattgatg agtcaaagta
      301 ttttgttgaa aaagattcta atgcgtttga agagtcttta gagtcagtga gtggaataaa
      361 atatataata aaagtaatga cttattctat tatgttaggt gggatggttg ttctttcatt
      421 aatcttgatt ctatggttaa gggaaagaat ttatgaaata ggtatattct tatctattgg
      481 aacatctaag atacaaatta taatgcaatt tatattcgag ttaatattca tatcgatacc
      541 aagtataata tcttccttat ttttaggaaa tgtattacta aaagtaattg tagatggatt
      601 cattaactca gaggattcaa tgatttccgg tggaagttta ataaataata gtagttttat
      661 gtcaaatata acaacacttg gacaaagtta tttaatatta ataagtatta ttgttttatc
      721 agttgtattt gcttcttcat taatgttaat caagaagcct aaag
//
LOCUS       DAFCLG010000252          754 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFCLG010000252 DAFCLG010000000
VERSION     DAFCLG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.265"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..627
                     /locus_tag="LND56_002810"
     CDS             <1..627
                     /locus_tag="LND56_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HBK6744665.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLS
                     YNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISN
                     AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          236 a          169 c          140 g          209 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actcttactc aaagattcca cgacatggga gccactcaaa
      241 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt tgatgagctg
      301 ttaagctaca acgaagaatt gcgaactgcg tatcattttt gtcaattatt acgctattat
      361 ttcgttaaac gaggtacgga aggctttcaa gaaaccctta aaacaatttc atctacgtta
      421 cctcagtcgt ttaaaaagaa attcacgatc ttccatcgct accaatcggg tatttcaaat
      481 gcctttaagg tgtcccactc aaacggcgtc actgaggggc tgaataacaa gatcaagctg
      541 attaaacgaa ttgcgtttgg ttatcgcaac ttttataact ttcgggcacg gatctattta
      601 caacaaggtc tgatctttga aaactaaaaa aatatgcggc gcacagaacg cgccgcatat
      661 cggactaagt tttattcaaa tttgtcttca ccaacacgat ttgacgaaga gccaaaaaag
      721 gctgcgacgg ttgtcacagc ctctctcgta tttt
//
LOCUS       DAFCLG010000253          747 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFCLG010000253 DAFCLG010000000
VERSION     DAFCLG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.266"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..81
                     /locus_tag="LND56_002811"
     tRNA            8..81
                     /locus_tag="LND56_002811"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="LND56_002812"
     tRNA            121..210
                     /locus_tag="LND56_002812"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="LND56_002813"
     tRNA            225..296
                     /locus_tag="LND56_002813"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="LND56_002814"
     tRNA            312..384
                     /locus_tag="LND56_002814"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="LND56_002815"
     tRNA            394..466
                     /locus_tag="LND56_002815"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="LND56_002816"
     tRNA            502..577
                     /locus_tag="LND56_002816"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="LND56_002817"
     tRNA            579..659
                     /locus_tag="LND56_002817"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="LND56_002818"
     tRNA            666..739
                     /locus_tag="LND56_002818"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
BASE COUNT          168 a          169 c          203 g          207 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaat
//
LOCUS       DAFCLG010000254          746 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFCLG010000254 DAFCLG010000000
VERSION     DAFCLG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.267"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..627
                     /locus_tag="LND56_002819"
     CDS             <1..627
                     /locus_tag="LND56_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6744666.1"
                     /translation="FCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVV
                     LSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIER
                     FEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKA
                     FYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          275 a          142 c          130 g          199 t
ORIGIN      
        1 ttctgtgatt tacttcaaga aaaaggaaaa ggccgtttca agcaaccaga aaaaatcgct
       61 aaagcaattc aacgtgcaat taccatgagt taccgcctag gtgctcttgc acaagaatca
      121 attaatgttg ttttaagtgt tctcgtacga gaaatacgag cacttgaaaa aaatatcaaa
      181 gaattagata aagccatcga acaagtaatt gttgtcattc cagaatatca atgcttaacc
      241 agtattccag gcgttggtaa agtctatgct gccggtttaa tcgctgaaat tggtcaaatc
      301 gaaagatttg aagatcagac aaaattggca aaatacgctg gattaagttg gaaagtgaac
      361 caatctggaa actatcagtc gcaaaataca cctctgacaa aacaaggaaa tcgatatttt
      421 agatactact tagttgaagc tgccaactct gtaaaaaatt atcttcctga atacaaagct
      481 ttttatcaaa gtaaatataa ggaggttcca aagcatcaac acaaacgtgc actcgtccta
      541 accgcaagaa aatttacgcg tctggtggat acgctactac gtaaacacca actctatacg
      601 ccaccaagga gtgtgataga gaaataacaa tccgttattg acgcaaatcc ttgaatcaac
      661 ccgaaaaaaa tttgattttg atcgggtcta gtttcgtgcg cttcaaaaca tattactcaa
      721 taaagaaaat cttaaatttc atcttg
//
LOCUS       DAFCLG010000255          714 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFCLG010000255 DAFCLG010000000
VERSION     DAFCLG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.268"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..622
                     /locus_tag="LND56_002820"
     CDS             5..622
                     /locus_tag="LND56_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744667.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          268 a           91 c          133 g          222 t
ORIGIN      
        1 aaccttgggt tttcaagtgc tttttggttc attaatctta accatagtgt ttttaaagtg
       61 gaaaacaagc aacaaaaaaa gtatgtggag ccttgtagga atagttctta ttatttttac
      121 tgtgactgca gcaacagaag caaattattc gcataaaatt cagtcgtata aaggtcgttt
      181 agttatgacc aaaaaagatg ttacacgtta tacatcctca agaactggag gcctatatga
      241 ctatgcacca ttagatgcat tgaaatataa agatcattta aagaaacatg aagtaaaagt
      301 tggtgatcgg tgggagaatg catctcataa agcatataat tccaaaatag tctatacagt
      361 agaagatgcc gatgagcaaa atatcacatt acctgtattc aattattggg gaacagttgt
      421 gaaagtaaat gggaaaaaga gcactataga aaatgacaat ggtttagtgt ctattaaagg
      481 tacggggaaa agaactatag ttgaggtttc atctacgtat ccaactacga tgattctatc
      541 tttattaatt agttcaataa cttttatatt cttatttaca cgttatttaa taagatgctg
      601 cggtgaaatg aaaatagatt agatagatat tataaatatg ttgattctat tttcatagca
      661 atggagaaaa tacattagca atatataaaa gaaggtatca agagtcacag tgaa
//
LOCUS       DAFCLG010000256          713 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFCLG010000256 DAFCLG010000000
VERSION     DAFCLG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.269"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..448)
                     /locus_tag="LND56_002821"
     CDS             complement(<1..448)
                     /locus_tag="LND56_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6744668.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          123 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aac
//
LOCUS       DAFCLG010000257          706 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFCLG010000257 DAFCLG010000000
VERSION     DAFCLG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.270"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..412
                     /locus_tag="LND56_002822"
     CDS             83..412
                     /locus_tag="LND56_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6744669.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            402..689
                     /locus_tag="LND56_002823"
     CDS             402..689
                     /locus_tag="LND56_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6744670.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          269 a           78 c          134 g          225 t
ORIGIN      
        1 cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag taattacata
       61 ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca attcaatcat
      121 gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag caaagttggt
      181 agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg attttagcga
      241 agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc ttgtagaatt
      301 taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag atacacttac
      361 taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat gaaatcgttc
      421 cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg aaagaaaaat
      481 ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa caaaaaactg
      541 gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca tatagagtag
      601 cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg catgaaaact
      661 tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttct
//
LOCUS       DAFCLG010000258          695 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFCLG010000258 DAFCLG010000000
VERSION     DAFCLG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.271"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..627)
                     /locus_tag="LND56_002824"
     CDS             complement(52..627)
                     /locus_tag="LND56_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6744671.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          228 a          151 c          125 g          191 t
ORIGIN      
        1 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
       61 ttttcttttt gaatatatca agcgttgggg caacatagtg atgactaatg aaacaacgac
      121 gatagacaac aagcgatcaa ggtagtctgt acccgcctgt accaagatca cacttgttgt
      181 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
      241 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
      301 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
      361 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
      421 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
      481 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
      541 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
      601 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
      661 tcataatcgt ttcttcttgt gcatttaaaa gaacg
//
LOCUS       DAFCLG010000259          690 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFCLG010000259 DAFCLG010000000
VERSION     DAFCLG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.272"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..461)
                     /locus_tag="LND56_002825"
     CDS             complement(<1..461)
                     /locus_tag="LND56_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6744672.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          219 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAFCLG010000260          670 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFCLG010000260 DAFCLG010000000
VERSION     DAFCLG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.273"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..417
                     /locus_tag="LND56_002826"
     CDS             154..417
                     /locus_tag="LND56_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6744673.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="LND56_002827"
     CDS             417..>670
                     /locus_tag="LND56_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6744674.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAFCLG010000261          667 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFCLG010000261 DAFCLG010000000
VERSION     DAFCLG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.274"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            246..>667
                     /locus_tag="LND56_002828"
     CDS             246..>667
                     /locus_tag="LND56_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HBK6744675.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQF"
BASE COUNT          221 a          113 c          108 g          225 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctctaataat
       61 tacacacaca caatttttta aggtcttaaa accatcatat atgcaaaaga agctaacctt
      121 tattactaaa ttagacccgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca
      181 tgttggtaaa tcaacaaaaa aacgcatata gtagtctcaa aactcccaag aaatgaggac
      241 tacatatgcg ttcatcaaaa tatactgaac attttgactt agccaatcct gttactaaag
      301 tcgatgatat ccccgattat gaaatgtaca gtcaaacaat cgatagctta aataagcgtt
      361 ttggcaatcg tgttttaaaa ggcatagaaa tcggttacat cgcttctgaa aaggatcgga
      421 tcatcgacta tttagccgat aaagattacg atttaaaatt actcagtgtg catcataatg
      481 gtcaatttga ttatttagat gacgaagtca aagatatgga tcctgctatt gtgattcctc
      541 agtattttgc acaactttca gaagcactag ttgttataga agcagatgtc tttgctcatt
      601 ttgactatgg ttttcgggta tttggtctat ccgttgctga atttaaacaa tatgaagcgc
      661 agttttt
//
LOCUS       DAFCLG010000262          607 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFCLG010000262 DAFCLG010000000
VERSION     DAFCLG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.275"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..234
                     /locus_tag="LND56_002829"
     CDS             73..234
                     /locus_tag="LND56_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK6744676.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>607
                     /locus_tag="LND56_002830"
     CDS             253..>607
                     /locus_tag="LND56_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6744677.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVS"
BASE COUNT          249 a           58 c          106 g          194 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcac
//
LOCUS       DAFCLG010000263          607 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFCLG010000263 DAFCLG010000000
VERSION     DAFCLG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.276"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>607
                     /locus_tag="LND56_002831"
     CDS             <1..>607
                     /locus_tag="LND56_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744678.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDKEAAER
                     GKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEH"
BASE COUNT          225 a          107 c          132 g          143 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tgcaaacatt ttggaatgga cacgtcggag aaagctattc
      421 cttatattgc agcatggacg aagaatggtc agacgttgga tgataaggaa gcggcggaac
      481 gtgggaaaat catgaatgat gtcagccgtg tcgcaaatga attcattcaa accatttcta
      541 atgaaatcaa tcaatacaga gaaagagaac cagagatcca accagcagaa gtgaagcagg
      601 agcataa
//
LOCUS       DAFCLG010000264          604 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFCLG010000264 DAFCLG010000000
VERSION     DAFCLG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.277"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..323
                     /locus_tag="LND56_002832"
     CDS             <1..323
                     /locus_tag="LND56_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744679.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          216 a          106 c           74 g          208 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg tattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
      421 ttttattaaa ctttttaagt ttctattcta tcaaatattt aaacaatctc tgcatccgaa
      481 aagactacct cgtatagttt tactaacaat atttttatta gcgtgttcaa atcctgattt
      541 cttgtatcaa ttattttctg ttcactttca ataagaaaaa agtctttact tccatggata
      601 ataa
//
LOCUS       DAFCLG010000265          591 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFCLG010000265 DAFCLG010000000
VERSION     DAFCLG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.278"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            26..>591
                     /locus_tag="LND56_002833"
     CDS             26..>591
                     /locus_tag="LND56_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBK6744680.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          210 a          101 c          117 g          163 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DAFCLG010000266          548 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFCLG010000266 DAFCLG010000000
VERSION     DAFCLG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.279"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>548)
                     /locus_tag="LND56_002834"
     CDS             complement(<1..>548)
                     /locus_tag="LND56_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6744681.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DAFCLG010000267          542 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFCLG010000267 DAFCLG010000000
VERSION     DAFCLG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.280"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          196 a           73 c           65 g          208 t
ORIGIN      
        1 ggaaaacgta gacgtaattt tctacgtttt ccttttcata tatattaaat attacggaaa
       61 attatataaa ctaatttagt gttttgtttg taattcctaa ttttgctcca cttttagata
      121 atttttaggt ctatttaact catcagacat tctatgctca tttttctcca aatttgtttc
      181 aactctttcg tacttaattt tgcgtatttg aaaattcttc ttttagagta cttttattac
      241 gatactagta tgatatatat ctatagcaaa cagttaaaat cttcctaaaa taagcgaata
      301 gcattcctaa aacctataaa atttttagat gaagcgacaa gtggacaatt aataaactac
      361 ttgaacatga aattatggat cacctttcaa atttagaaaa tggaattctt atacttattg
      421 cgcaacattt attgattgca aaattatgtg acaaaaaaaa ctagtagaag aaagaataat
      481 ggcttgataa taaagtacta cctattaaag gtagtacttt atttaattta gtataaatta
      541 ac
//
LOCUS       DAFCLG010000268          526 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFCLG010000268 DAFCLG010000000
VERSION     DAFCLG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.281"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..354
                     /locus_tag="LND56_002835"
     CDS             <1..354
                     /locus_tag="LND56_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6744682.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="LND56_002836"
     CDS             355..>526
                     /locus_tag="LND56_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744683.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAFCLG010000269          523 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFCLG010000269 DAFCLG010000000
VERSION     DAFCLG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.282"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            27..>523
                     /locus_tag="LND56_002837"
     CDS             27..>523
                     /locus_tag="LND56_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBK6744684.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          191 a           96 c           86 g          150 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DAFCLG010000270          519 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFCLG010000270 DAFCLG010000000
VERSION     DAFCLG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.283"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..461
                     /locus_tag="LND56_002838"
     CDS             <1..461
                     /locus_tag="LND56_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6744685.1"
                     /translation="DLDALQVVSVVSFYESFIDGADPLDMQGYLQTQLTTEILAVELA
                     QSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLGQRLLDEANRYAKT
                     KQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPILEKEI"
BASE COUNT          184 a           75 c          100 g          160 t
ORIGIN      
        1 atgatctaga tgctttacaa gttgtaagtg ttgtcagttt ttatgaatcg tttatcgatg
       61 gcgcagatcc acttgatatg caaggatatt tacagacaca attgacaact gaaatattgg
      121 cagtagaatt ggcacagtca accagtaaat ttattggtat taaggatcat caaatactca
      181 ttggttatat gaaagtcaat gatgaaaaag atgctataga gattcaacgg ctctatctgc
      241 tcaaagatta tcaaaacaaa gggttagggc aacggttact cgatgaagcg aatcgttatg
      301 ctaaaacgaa acaaaaacgt tatttacgtt tgactgttta tgaaaaaaat catgcaggta
      361 ttcgttttta tgaacgtcat ggatttaaaa aaatcgggat caaacatttt cctttaggaa
      421 aacaagatcg tatttgtccg attttagaaa aagaaatcta aaaaaacgtc ctcacttttg
      481 atgctgagga cgttttttta gactggtaat ggaaccata
//
LOCUS       DAFCLG010000271          516 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFCLG010000271 DAFCLG010000000
VERSION     DAFCLG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.284"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          231 a           71 c           83 g          131 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaa
//
LOCUS       DAFCLG010000272          509 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFCLG010000272 DAFCLG010000000
VERSION     DAFCLG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.285"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="LND56_002839"
     CDS             complement(<1..132)
                     /locus_tag="LND56_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6744686.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAFCLG010000273          500 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFCLG010000273 DAFCLG010000000
VERSION     DAFCLG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.286"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>500
                     /locus_tag="LND56_002840"
     CDS             <1..>500
                     /locus_tag="LND56_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HBK6744687.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DAFCLG010000274          473 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFCLG010000274 DAFCLG010000000
VERSION     DAFCLG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.287"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>473
                     /locus_tag="LND56_002841"
     CDS             <1..>473
                     /locus_tag="LND56_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6744688.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DAFCLG010000275          468 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFCLG010000275 DAFCLG010000000
VERSION     DAFCLG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.288"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>468
                     /locus_tag="LND56_002842"
     CDS             <1..>468
                     /locus_tag="LND56_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK6744689.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAFCLG010000276          467 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFCLG010000276 DAFCLG010000000
VERSION     DAFCLG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.289"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..>467
                     /locus_tag="LND56_002843"
     CDS             123..>467
                     /locus_tag="LND56_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744690.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          206 a           63 c           69 g          129 t
ORIGIN      
        1 ttagctaaag ctgaacaata tagacgatct ttagaattgt caaaagatta aatatataaa
       61 aagccttcgg gctttttctt ttaacaatta ataggaacgt atattcgaaa ggaatagaaa
      121 taatgtggtt agaaaaaata aatgacaaaa aatttaagta caccgaacgt tatacggatc
      181 ccttaactga aaaaaagaga aaagtgtccg ttactttatc tagcaattca cgccaagctt
      241 ggaatcaagc taacttactt ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag
      301 aattaccact aacttttgga gaattaaaaa ctaaatggga tgaaaaatac aagccaactg
      361 taaaagaaag ttcctatcga acaactcagg tttatctatc attaatttca aaatatataa
      421 aagatgatgt acttgtaaaa aacgttaata gtaatcttat acaagat
//
LOCUS       DAFCLG010000277          455 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFCLG010000277 DAFCLG010000000
VERSION     DAFCLG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.290"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="LND56_002844"
     CDS             complement(<1..430)
                     /locus_tag="LND56_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6744691.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          131 a           90 c           58 g          176 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatt
//
LOCUS       DAFCLG010000278          427 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFCLG010000278 DAFCLG010000000
VERSION     DAFCLG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.291"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>427)
                     /locus_tag="LND56_002845"
     CDS             complement(<1..>427)
                     /locus_tag="LND56_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744692.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          125 a           76 c           87 g          139 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaa
//
LOCUS       DAFCLG010000279          426 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFCLG010000279 DAFCLG010000000
VERSION     DAFCLG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.292"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            183..>426
                     /locus_tag="LND56_002846"
     CDS             183..>426
                     /locus_tag="LND56_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6744693.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
BASE COUNT          144 a           52 c           73 g          157 t
ORIGIN      
        1 ttttttttct accctaaatt tctatttaca caaaatattt tacgctatct tttattttaa
       61 aatatgggat attatgataa attaactatg gatgatattt gaaaaaaaca tactgtatat
      121 ttgatttaat ttttattttt aagctcatat cgtaagaatg ggacttgtag tctcacttct
      181 ttatggaggt gataattatg aacgttggtt ttattggagc cggcaaggta ggttgttcat
      241 tcgggaagta tttccaggaa cataaaatac aagtaacagg attttatagt aaaagcgaag
      301 actcgtcatt agcagcttcg aattttacat ctagtaaaca atatttaaac ttaagagaat
      361 tagtagatga aaatgatact attttcatta cgactcctga tggtcaaatt caggaggtat
      421 ggcaag
//
LOCUS       DAFCLG010000280          407 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.293, whole genome shotgun sequence.
ACCESSION   DAFCLG010000280 DAFCLG010000000
VERSION     DAFCLG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.293"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..226
                     /locus_tag="LND56_002847"
     CDS             <1..226
                     /locus_tag="LND56_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK6744694.1"
                     /translation="DRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVG
                     SEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT          160 a           75 c           70 g          102 t
ORIGIN      
        1 tgatagagat tactttactc aggcagactt gatcattaca ctttgtggtg atgccaaaga
       61 caagtgccct gccgttccaa acaatcacga acaccttcac tgggacctaa aagatcctgc
      121 acaagctgtc gggagcgaag aagaaatatt aacggaattc agaaaagtca gagaaggaat
      181 caaaagaaaa gttttagact taaatcaacg tacatcgatc gtataaagaa gtattcaaaa
      241 tgatttttca agcactaaga aacaatgtta aaaaaatagc tttccatatt ttacaaaatt
      301 gtgccttatt gccgacaaat tgtctcttga acaccgttta ccagtagtca agacatcgta
      361 agcaactata aaaaattata agaaactcaa aataaaagga tgtgcca
//
LOCUS       DAFCLG010000281          384 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFCLG010000281 DAFCLG010000000
VERSION     DAFCLG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.294"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          145 a           45 c           81 g          113 t
ORIGIN      
        1 tcattggtcg ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc
       61 ttttatggta gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat
      121 atgaatcgag atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta
      181 cagtttcgtt cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa
      241 gagggcggca catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag
      301 gctatcctac tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt
      361 cattaaatcc cttcaagtga tgaa
//
LOCUS       DAFCLG010000282          373 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFCLG010000282 DAFCLG010000000
VERSION     DAFCLG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.295"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..245
                     /locus_tag="LND56_002848"
     CDS             <1..245
                     /locus_tag="LND56_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744695.1"
                     /translation="YKSASKKLTINIDELADQVLSQLNETQEEYYTMPANDAKDNVSH
                     RFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          129 a           69 c           68 g          107 t
ORIGIN      
        1 attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac
       61 tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag
      121 ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca
      181 cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc
      241 tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa
      301 ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg
      361 tcaacccctt ttt
//
LOCUS       DAFCLG010000283          368 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFCLG010000283 DAFCLG010000000
VERSION     DAFCLG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.296"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..300
                     /locus_tag="LND56_002849"
     CDS             <1..300
                     /locus_tag="LND56_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744696.1"
                     /translation="NKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT          141 a           53 c           82 g           92 t
ORIGIN      
        1 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
       61 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
      121 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
      181 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
      241 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
      301 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
      361 atgaccga
//
LOCUS       DAFCLG010000284          368 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFCLG010000284 DAFCLG010000000
VERSION     DAFCLG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.297"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>368
                     /locus_tag="LND56_002850"
     CDS             82..>368
                     /locus_tag="LND56_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK6744697.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DAFCLG010000285          368 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFCLG010000285 DAFCLG010000000
VERSION     DAFCLG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.298"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..300
                     /locus_tag="LND56_002851"
     CDS             <1..300
                     /locus_tag="LND56_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744698.1"
                     /translation="QKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT          143 a           51 c           80 g           94 t
ORIGIN      
        1 caaaagaata ttgttcctgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
       61 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccacag
      121 cataatcaag taattttaaa aggaaaaagt tatggaaaag agatccaaac ggcgttacct
      181 tcagaagaat ttgcgctagg aaattcattc cgattttcac ttgagacagt gtcagaagtg
      241 gcaaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgtgtataa
      301 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
      361 atgaccga
//
LOCUS       DAFCLG010000286          367 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFCLG010000286 DAFCLG010000000
VERSION     DAFCLG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.299"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..354
                     /locus_tag="LND56_002852"
     CDS             79..354
                     /locus_tag="LND56_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744699.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          158 a           57 c           53 g           99 t
ORIGIN      
        1 tttcagtatc cttcttgttt tcatataacc aaaggcgcta tgatttccat atgaaaaaaa
       61 taaagtcagg tgaaaaaaat gaaacttgag gataaaaaag tcattttgaa gaaaatcaaa
      121 cgactaccag accaaataga tggctatacg atctgttctt tcaaaaaaga caggagtata
      181 aaagcttata cgcaaaacac agaaaaagga ataatctatt tcctacatga agaaggttac
      241 gatacccaaa cctttatgtt tactgaaaaa aaagaatttc ataaacagat gaaaaaactg
      301 attgaaaaag aatttccaag aagtcataaa ctctatgtga atcagcaatt ttaacttcta
      361 actgttc
//
LOCUS       DAFCLG010000287          349 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFCLG010000287 DAFCLG010000000
VERSION     DAFCLG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.300"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>349
                     /locus_tag="LND56_002853"
     CDS             <1..>349
                     /locus_tag="LND56_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744700.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGF"
BASE COUNT          138 a           65 c           53 g           93 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttc
//
LOCUS       DAFCLG010000288          349 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFCLG010000288 DAFCLG010000000
VERSION     DAFCLG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.301"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(44..>349)
                     /locus_tag="LND56_002854"
     CDS             complement(44..>349)
                     /locus_tag="LND56_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744701.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          101 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggt
//
LOCUS       DAFCLG010000289          342 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFCLG010000289 DAFCLG010000000
VERSION     DAFCLG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.302"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..270
                     /locus_tag="LND56_002855"
                     /pseudo
     CDS             1..270
                     /locus_tag="LND56_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          124 a           46 c           64 g          108 t
ORIGIN      
        1 aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata aaccttttcc
       61 ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac gatttatatt
      121 ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt tggtttagct
      181 aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat tgattctcct
      241 caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg atagggtctc
      301 acgctctttg tttttcgcca tttaaagatt gaaatttttg cc
//
LOCUS       DAFCLG010000290          332 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFCLG010000290 DAFCLG010000000
VERSION     DAFCLG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.303"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>332
                     /locus_tag="LND56_002856"
     CDS             <1..>332
                     /locus_tag="LND56_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744702.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAFCLG010000291          320 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFCLG010000291 DAFCLG010000000
VERSION     DAFCLG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.304"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>320
                     /locus_tag="LND56_002857"
     CDS             <1..>320
                     /locus_tag="LND56_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6744703.1"
                     /translation="KKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDLEFKNKEILKL
                     EKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNIS
                     QILS"
BASE COUNT          144 a           29 c           42 g          105 t
ORIGIN      
        1 taaaaaaatg agtttaaaag ttagctctaa tgatgaagtt ggagaattaa aacaacaaat
       61 taatgatttg tattctactt tattaagaac aattgacgat ttggaattta aaaataaaga
      121 aattttaaaa ttagaaaagt taaaatatga cttttttaaa ggtgcatccc atgaacttaa
      181 aacccctctt gctagtctta aaataatact ggaaaacatg aaatacaata ttggaaaata
      241 taaagaaaga gatatttata ttaatgaatg tattgaaatc gttgatagtt taacaaaaaa
      301 tatttctcaa atattatcag
//
LOCUS       DAFCLG010000292          315 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.305, whole genome shotgun sequence.
ACCESSION   DAFCLG010000292 DAFCLG010000000
VERSION     DAFCLG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.305"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(98..182)
                     /locus_tag="LND56_002858"
     tRNA            complement(98..182)
                     /locus_tag="LND56_002858"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Leu,seq:caa)
     gene            complement(203..273)
                     /locus_tag="LND56_002859"
     tRNA            complement(203..273)
                     /locus_tag="LND56_002859"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Cys,seq:gca)
BASE COUNT           93 a           77 c           63 g           82 t
ORIGIN      
        1 cacaatccgg tgcgttaacc acttcgccac attcgccatc ataaaagatg ttatttaatt
       61 tctcaataaa aaactaagat gaagacatct tagtcaatac cggcggccgg ggtcgaaccg
      121 gcacgccctc aacgggcact ggattttgag tccagcgcgt ctgccaattc cgccacgccg
      181 gcaagttgat ttattagtac taaggcggta accggatttg aaccggtgat gaaggttttg
      241 cagacctctg ccttaccact tggctatacc gccattatac tattatcaat ctataaaata
      301 aagaataaat tatag
//
LOCUS       DAFCLG010000293          309 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFCLG010000293 DAFCLG010000000
VERSION     DAFCLG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.306"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>309
                     /locus_tag="LND56_002860"
     CDS             <1..>309
                     /locus_tag="LND56_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6744704.1"
                     /translation="EDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISN
                     AVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVS
                     "
BASE COUNT          136 a           16 c           52 g          105 t
ORIGIN      
        1 agaagacata ttaaaaaagt atgaaatatt agcaaatcaa aagaaaataa ctgtcaataa
       61 ttatatatta gacgagaatg tttatatagg aaaaacagct ttaaagatta ttctatctaa
      121 tttgattagt aatgcggtaa aatatactga tgtaaatggg gtaattaata ttgggatagt
      181 taatgattgg ttatatatag agaattcata cggtaacaat aaaatttcga atatggataa
      241 aatatttgat gttaaatttg atttaaataa ggaaaatagt aatggattag gtttatatat
      301 tgtaagtaa
//
LOCUS       DAFCLG010000294          289 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.307, whole genome shotgun sequence.
ACCESSION   DAFCLG010000294 DAFCLG010000000
VERSION     DAFCLG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.307"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..>289
                     /locus_tag="LND56_002861"
     CDS             59..>289
                     /locus_tag="LND56_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBK6744705.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFL"
BASE COUNT           93 a           47 c           62 g           87 t
ORIGIN      
        1 atggtaaact tgaaaatttt aaaaatatgt ggtatagttg atagtagagg tgaactgtat
       61 gccaacaact ttagcttcaa tcaagaaaga tttagaaggt cgtatcggca gcccaatcat
      121 gttggttgct cagacaggaa gaaagcgtca aacagaacgc cgcggcgttt taacagaaac
      181 ctatccttca gtattcgttg ttgatttgga tcctgaagaa aattcttttg aacgagtatc
      241 ttacagctat tcagatgtac ttactcggac agttgagatc gaattttta
//
LOCUS       DAFCLG010000295          278 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.308, whole genome shotgun sequence.
ACCESSION   DAFCLG010000295 DAFCLG010000000
VERSION     DAFCLG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.308"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>278)
                     /locus_tag="LND56_002862"
     CDS             complement(<1..>278)
                     /locus_tag="LND56_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744706.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPER"
BASE COUNT           94 a           49 c           42 g           93 t
ORIGIN      
        1 tatctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt
       61 gcgtccggca cgtcagtcac gatatcaaac aaaaagtata catccttctt cattctagtt
      121 ttcgcagcag gaattttgaa atagttctta ttagaatagt agagattgat taataagcta
      181 tcttcgattg ctaaaaagaa aacttctgaa ttccatactt tataaaaatc tttgataaat
      241 ctattcgaag ggtcaaattt aaaccataat tgtgtctc
//
LOCUS       DAFCLG010000296          277 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.309, whole genome shotgun sequence.
ACCESSION   DAFCLG010000296 DAFCLG010000000
VERSION     DAFCLG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.309"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..277
                     /locus_tag="LND56_002863"
                     /pseudo
     CDS             1..277
                     /locus_tag="LND56_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           46 c           53 g           69 t
ORIGIN      
        1 cattcgagaa tatgaactaa cggcttcagc atttgacaaa tgggtaaagc aatctaaaac
       61 gagtggttca ttcaaagaaa aagataatct tacgcctgaa caaaaagaat tgttagaact
      121 acgtaaaaga aaccagcaat tagaaatgga aaatgatatt ttaaagcaag cagcgctgat
      181 attcggacga agagacaagt aatcgatgcg aataagcatc tttaccctat atcagcgatg
      241 tgcagaatat taggtctatc acgtcagtcc tattatt
//
LOCUS       DAFCLG010000297          276 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.310, whole genome shotgun sequence.
ACCESSION   DAFCLG010000297 DAFCLG010000000
VERSION     DAFCLG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.310"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>276
                     /locus_tag="LND56_002864"
     CDS             <1..>276
                     /locus_tag="LND56_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744707.1"
                     /translation="KEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAI
                     LMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLC"
BASE COUNT           88 a           57 c           60 g           71 t
ORIGIN      
        1 caaagaaaag ttaactattt tagaattaaa acgtacccgt tttctctgcc atgattgtgg
       61 aaggaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact
      121 gaagcaggcg attctcatgg aactgtacga aaatcaatca cgtaaactaa tcgctaagaa
      181 gtacttcgtc tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca
      241 gccacgtatg aacttccttc cgacggtgtt gtgtat
//
LOCUS       DAFCLG010000298          245 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.311, whole genome shotgun sequence.
ACCESSION   DAFCLG010000298 DAFCLG010000000
VERSION     DAFCLG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.311"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..245
                     /locus_tag="LND56_002865"
                     /pseudo
     CDS             1..245
                     /locus_tag="LND56_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           99 a           45 c           49 g           52 t
ORIGIN      
        1 tattcgagag tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc
       61 aacgggctca ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact
      121 aagaaagaaa aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat
      181 attcggacga aaagacaagt aattgatgcc aacaagcata aatattccat atcagcgatg
      241 tgcaa
//
LOCUS       DAFCLG010000299          241 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.312, whole genome shotgun sequence.
ACCESSION   DAFCLG010000299 DAFCLG010000000
VERSION     DAFCLG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.312"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..220)
                     /locus_tag="LND56_002866"
     CDS             complement(<1..220)
                     /locus_tag="LND56_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6744708.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAFCLG010000300          234 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.313, whole genome shotgun sequence.
ACCESSION   DAFCLG010000300 DAFCLG010000000
VERSION     DAFCLG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.313"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>234
                     /locus_tag="LND56_002867"
     CDS             <1..>234
                     /locus_tag="LND56_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6744709.1"
                     /translation="HKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNF
                     FKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT           77 a           46 c           36 g           75 t
ORIGIN      
        1 tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa
       61 acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt
      121 ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa
      181 agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa cacc
//
LOCUS       DAFCLG010000301          228 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.314, whole genome shotgun sequence.
ACCESSION   DAFCLG010000301 DAFCLG010000000
VERSION     DAFCLG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.314"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>228
                     /locus_tag="LND56_002868"
     CDS             <1..>228
                     /locus_tag="LND56_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6744710.1"
                     /translation="LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSI
                     SNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           82 a           42 c           41 g           63 t
ORIGIN      
        1 cttgaaacca gaaggccgta aggcagttga tattgaaaat tcgattaatg aatggcttca
       61 atccgtaccc aaaaatcttt tcaaatcaat tacctttgat tgtggaaaag aattctctaa
      121 ttggaaaagt attagtaata cgaatgatat tgatatttat ttcgcagacc caggaacgcc
      181 ttcccaacgg ggattaaatg aacattcaaa cggactcctc cgaaaaga
//
LOCUS       DAFCLG010000302          224 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.315, whole genome shotgun sequence.
ACCESSION   DAFCLG010000302 DAFCLG010000000
VERSION     DAFCLG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.315"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>224
                     /locus_tag="LND56_002869"
     CDS             <1..>224
                     /locus_tag="LND56_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBK6744711.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAFCLG010000303          218 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.316, whole genome shotgun sequence.
ACCESSION   DAFCLG010000303 DAFCLG010000000
VERSION     DAFCLG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.316"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>218
                     /locus_tag="LND56_002870"
     CDS             <1..>218
                     /locus_tag="LND56_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBK6744712.1"
                     /translation="ESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGST
                     LQEVKAASEKQEAARHLGICPKCQEQEV"
BASE COUNT           78 a           45 c           45 g           50 t
ORIGIN      
        1 gaaagctact tgttaaagat cggtgaacga aaaggaacac aaaccacttt tctagccaat
       61 atccaaaaat ttgtctctca cttattggaa gtcgtaccag ggcaaatcca atccactgat
      121 tttggatcga cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat
      181 ttaggaattt gtccaaaatg ccaggaacaa gaagtctt
//
LOCUS       DAFCLG010000304          218 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.denovo.317, whole genome shotgun sequence.
ACCESSION   DAFCLG010000304 DAFCLG010000000
VERSION     DAFCLG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.denovo.317"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>218
                     /locus_tag="LND56_002871"
     CDS             <1..>218
                     /locus_tag="LND56_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6744713.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT           66 a           39 c           44 g           69 t
ORIGIN      
        1 tcttttattg cgaaaaattg tcaggtatct ttgaccaccg ttattcgtac gttgaaagag
       61 tttaaaagct acttaccaaa gcaatctaag aggattctcc ccagagtatt gatggttgat
      121 gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc agatggcgaa
      181 acaggacaat taattgatgt cttgcctacg cgtaaatt
//
LOCUS       DAFCLG010000305         7781 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.114, whole genome shotgun sequence.
ACCESSION   DAFCLG010000305 DAFCLG010000000
VERSION     DAFCLG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.114"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            246..1214
                     /locus_tag="LND56_002872"
     CDS             246..1214
                     /locus_tag="LND56_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6744714.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..2238
                     /gene="vanA"
                     /locus_tag="LND56_002873"
     CDS             1207..2238
                     /gene="vanA"
                     /locus_tag="LND56_002873"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6744715.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2244..2852
                     /locus_tag="LND56_002874"
     CDS             2244..2852
                     /locus_tag="LND56_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK6744716.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3280..4191
                     /locus_tag="LND56_002875"
     CDS             3280..4191
                     /locus_tag="LND56_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK6744717.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4344..4829
                     /gene="vanZ"
                     /locus_tag="LND56_002876"
     CDS             4344..4829
                     /gene="vanZ"
                     /locus_tag="LND56_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK6744718.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5116..5445)
                     /locus_tag="LND56_002877"
     CDS             complement(5116..5445)
                     /locus_tag="LND56_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6744719.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5750..6079
                     /locus_tag="LND56_002878"
     CDS             5750..6079
                     /locus_tag="LND56_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6744720.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6069..6356
                     /locus_tag="LND56_002879"
     CDS             6069..6356
                     /locus_tag="LND56_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6744721.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(6635..7198)
                     /locus_tag="LND56_002880"
     CDS             complement(6635..7198)
                     /locus_tag="LND56_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744722.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2567 a         1298 c         1547 g         2369 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1321 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1381 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1441 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1501 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1561 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1621 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1681 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1741 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1801 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1861 gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg accaaatcag gctgcagtac
     1921 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1981 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     2041 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     2101 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2161 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2221 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2281 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2341 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2401 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2461 atcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2521 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2581 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2641 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2701 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2761 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2821 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2881 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2941 tatagtagat agtattgaag atgtaaggca gagcgatatt gcggtcatta tctgcgtgcg
     3001 ctgcggcaag atagcctgat aataagactg atcgcataga ggggtggtat ttcacaccgc
     3061 ccattgtcaa caggcagttc agcctcgtta aattcagcat gggtatcact tatgaaaatt
     3121 catctacatt ggtgataata gtaaatccag tagggcgaaa taattgactg taatttacgg
     3181 ggcaaaacgg cacaatctca aacgagattg tgccgtttaa ggggaagatt ctagaaatat
     3241 ttcatacttc caactatata gttaaggagg agactgaaaa tgaagaagtt gtttttttta
     3301 ttgttattgt tattcttaat atacttaggt tatgactacg ttaatgaagc actgttttct
     3361 caggaaaaag tcgaatttca aaattatgat caaaatccca aagaacattt agaaaatagt
     3421 gggacttctg aaaataccca agagaaaaca attacagaag aacaggttta tcaaggaaat
     3481 ctgctattaa tcaatagtaa atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg
     3541 aatttatcta aacatgacga attaataaat ggatacgggt tgcttgatag taatatttat
     3601 atgtcaaaag aaatagcaca aaaattttca gagatggtca atgatgctgt aaagggtggc
     3661 gttagtcatt ttattattaa tagtggctat cgagactttg atgagcaaag tgtgctttac
     3721 caagaaatgg gggctgagta tgccttacca gcaggttata gtgagcataa ttcaggttta
     3781 tcactagatg taggatcaag cttgacgaaa atggaacgag cccctgaagg aaagtggata
     3841 gaagaaaatg cttggaaata cgggttcatt ttacgttatc cagaggacaa aacagagtta
     3901 acaggaattc aatatgaacc atggcatatt cgctatgttg gtttaccaca tagtgcgatt
     3961 atgaaagaaa agaatttcgt tctcgaggaa tatatggatt acctaaaaga agaaaaaacc
     4021 atttctgtta gtgtaaatgg ggaaaaatat gagatctttt attatcctgt tactaaaaat
     4081 accaccattc atgtgccgac taatcttcgt tatgagatat caggaaacaa tatagacggt
     4141 gtaattgtga cagtgtttcc cggatcaaca catactaatt caaggaggta aggatggcgg
     4201 aatgaaacca acgaaattaa tgaacagcat tattgtacta gcacttttgg ggtaacgtta
     4261 gctttttaat ttaaaaccca cgttaactag gacattgcta tactaatgat acaacttaaa
     4321 caaaagaatt agaggaaatt atattgggaa aaatattatc tagaggattg ctagctttat
     4381 atttagtgac actaatctgg ttagtgttat tcaaattaca atacaatatt ttatcagtat
     4441 ttaattatca tcaaagaagt cttaacttga ctccatttac tgctactggg aatttcagag
     4501 agatgataga taatgttata atctttattc catttggctt gcttttgaat gtcaatttta
     4561 aagaaatcgg atttttacct aagtttgctt ttgtactggt tttaagtctt acttttgaaa
     4621 taattcaatt tatcttcgct attggagcga cagacataac agatgtaatt acaaatactg
     4681 ttggaggctt tcttggactg aaattatatg gtttaagcaa taagcatatg aatcaaaaaa
     4741 aattagacag agttattatt tttgtaggta tacttttgct cgtattattg ctcgtttacc
     4801 gtacccattt aagaataaat tacgtgtaag atgtctaaat caagcaatct gatctttcat
     4861 acacataaag atattgaatg aattggatta gatggaaaac gggatgtggg gaaactcgcc
     4921 cgtaggtgtg aagtgagggg aaaaccggtg ataaagtaaa aagcttacct aacactatag
     4981 taacaaagaa agcccaatta tcaattttag tgctgaggaa ttggtctctt taataaattt
     5041 ccttaacgtt gtaaatccgc attttcctga cggtaccccc aaactaagat ttcatagata
     5101 gattttctga taactttaaa aaacacgaat agcggaaagg atgtcttcat tcgctaaaga
     5161 gtaataaact acttttccat cttgacgtga ttttgccatt ccatgttttt ttaaaaaacg
     5221 taaatggtgt gacgttgtag cgacactagc agtaagtatt tctgctaaat cacagacgca
     5281 catttcctta taggtttcta aagcatagaa aattttaatt ctagaaaaat cgctgaaaca
     5341 tttccctaac acaagtaagt ttgaaaaatc tttttgatcc aatttatttt ttattccatt
     5401 tactttttct ttatgtacga cagtcacttg gcaaactttt ttcattcatc tcacctcaca
     5461 ttcaaacgat tatttgaatg aagtatacct atttgatcac tcttttgtca atctacctta
     5521 attccaatac actattataa gcaatttcat gtattgaagt aaacttactt gcaagtgcgt
     5581 ttttgattcc aagttaaaaa catattatta ttctacataa tttcctaatc aaataaaact
     5641 cctttgaaaa ttgttctaaa ggagttttat tattttgtat ttctactaaa aatagcgtac
     5701 aatgtaatta ctaagtaatt acgaaatgta aaataatgga ggaaataata tgattacaaa
     5761 aacaagaaaa caaggaaatt ctattatgtt aacggtccct aaagaattcg atgtgcccaa
     5821 tggtgttgaa gtagaagcta agcttgtaga gaatggtatc ctttatgagt ttgtagagcc
     5881 taaaaaagaa ttttttgatt ttagtgaaga tattttggct gatattcttt cagaaggtta
     5941 tagcaaacaa gagatcttaa aagaatttag aaatagaaaa aatgcgttaa cttctgcatt
     6001 taaatctatt gctgaagata ctgttgcaaa ttctaaaccg atgacaaaag aggagttggc
     6061 agcagaaatt ggcttatgag attctacctt ctaaacacgt aataaagtat ctaaaaaaat
     6121 taaaagagaa aacattaaaa gaacaatttt taacgataat ttatgatgag atagcagttc
     6181 gtcctcattc gggagaacaa aaaacgggtg atttatcagg aatttgggct attggattca
     6241 agtatgcagg tacgacatat cgcgttgcat atgaaattaa agataatgca gtgataccta
     6301 acttactatg tggaacgcat gaaaattttt atgaacaatt aaaaaagatt aggtaagaac
     6361 ccaatgtttc aacttgagag gatagacgac actttaaaag ataattttta acagctgaga
     6421 gttgtaatac cctataattt taaaccacta acctatatat ttatgaaaaa tagaaaaaat
     6481 taaaacgcat tttaagcctc tttcaaatta aaagcttcat ccttatgcac aaagagggct
     6541 taaaatgcgt tattttatat tgttttttag taataataaa aaaatcactc taattctccc
     6601 ataaatatta tagattccta aagattaaaa tagattattc cctattgtct tttaagtagc
     6661 ggtagagtgt tgccttgcta ataccagtca tttccgtaat ttcttttacg ctataaacct
     6721 gacttttata caattttact gctcgatcaa catctttctg gttcactcgt ggacggccac
     6781 ctttacgacc tctcgcacgt gcacttttca aaccttcttt ggtacgttca acaattaaat
     6841 cacgttcgaa ctgactaaat gcttggaaga cagtcatcat taagcgtccg tgaggcgtgc
     6901 tggtatcgaa gttttctttc aagctcacca gcttaacatg gtgttcttca aaataggtaa
     6961 caaggtcaat caaatccttt gtactacgac ccaatcgaga aaaactttct acaattaccg
     7021 tatctcccgg acgaatttta tcttttaaac gatttagttg gggacgaacc gctttggttc
     7081 ccgtcatttt ctcagttagg atttcattac aattttgttc attgagtaag tctaattgac
     7141 gatttagttc ttgtgaacgt gtactcactc gggcataacc ataaatgtat ccactcatgc
     7201 ttttcttcct ttcgtacttt aattctggct tgattgtatc ataaaaggga gttatagata
     7261 catagttaat gcaacaagtt atgatacaaa atatgacctc ttttatttaa taaaagaggt
     7321 catttcattt tgtctcataa tcgattgttt ttgatacgag tgtgtatacg aaataaacaa
     7381 aaaaatctta agtaaatttc tagataccta atttaatcct aactagtgta tcctaaagtt
     7441 aataacgagt ttatcacaat gaaaattata aaatgctccc atattagata taacatgcaa
     7501 tatttgaatt agaactacaa aatttattcg ttttataaag taaataatgt tgagatacta
     7561 aggaatagaa tattataagg aattgagctg aatgtattgg ataaaaaaag aataacttta
     7621 ttagagatgg actataaaaa tgctcgtaat tttcttctga agtctgaaag ctatttgaat
     7681 gctcagctac cagaatatat tagttttgac gaaactttaa aatctgctaa aaaagtttta
     7741 acttcgccgt ttgtataatt aagcgagaca aataaaaaag c
//
LOCUS       DAFCLG010000306         6262 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.129, whole genome shotgun sequence.
ACCESSION   DAFCLG010000306 DAFCLG010000000
VERSION     DAFCLG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.129"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            239..475
                     /locus_tag="LND56_002881"
     CDS             239..475
                     /locus_tag="LND56_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744723.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            482..2434
                     /locus_tag="LND56_002882"
     CDS             482..2434
                     /locus_tag="LND56_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6744724.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2506..3003)
                     /gene="dfrG"
                     /locus_tag="LND56_002883"
     CDS             complement(2506..3003)
                     /gene="dfrG"
                     /locus_tag="LND56_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6744725.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3365..4405
                     /locus_tag="LND56_002884"
     CDS             3365..4405
                     /locus_tag="LND56_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBK6744726.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            4503..5876
                     /gene="tet(L)"
                     /locus_tag="LND56_002885"
     CDS             4503..5876
                     /gene="tet(L)"
                     /locus_tag="LND56_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK6744727.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            complement(5925..6125)
                     /locus_tag="LND56_002886"
     CDS             complement(5925..6125)
                     /locus_tag="LND56_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744728.1"
                     /translation="MPIFILSVLSSLPKARLSKNKIFIVFRFSSVTDKTTQNKKDTRE
                     VSRIFYSAIAPFNGTKRFEFLL"
BASE COUNT         2064 a          993 c         1167 g         2038 t
ORIGIN      
        1 ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta aacctaaatg agtatcaaat
       61 tgcaactgaa tttgatactc gttttttatt gttaaatcaa cgtttgttgt taatttacac
      121 cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt gaaataatta ttgtaggaat
      181 gcaaattggt gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc attttcctat
      241 gcaatttctt gagccaaaaa ataagaacgc taagtctgtg gattgggaaa tatcagagca
      301 agtaagggtg atagttaagc aatacgctga atatgcagag cgtactgaaa gcgaagcagt
      361 ggatgaattc ttattgaaca ttttagacga taagaagttt attgaatgga ttgccaacaa
      421 gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg gttgatagac
      481 aatgaccaag cttaaaagat tagccacgaa ggaagatgaa attgtagatg ttaaaattcc
      541 gatttcaaac gaagaattaa aagatagggc taaacaatat gatttattaa ctccaaaacg
      601 atttgcaaca aggtataaca aaatgttgtt cttgccaacg tcttttaaat ggaacggttc
      661 tgaatatccc attcaatata attactgcat caatccgttt tgttgtaatt ttgggaaaga
      721 acagcacaaa ttcaaagatg taaaagggaa accaagtcga tataaaatga ctggtagttc
      781 caaagataaa ggtcataaag gtatgtactg caatgacaat cctattggca gaggtgtatc
      841 gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa ttaaacgcct
      901 tatagaaatc aatagcatcc aagatgttga gccagattat caatttcata aagaaggctg
      961 ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa gggggaaaag
     1021 taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta atgttctacc
     1081 aaaaagagaa gaaaccacta cttatcacca acaaaagaac accattcttc caatgttcgc
     1141 taagatggtt gttggaaggg tttcagtcag tcgtacttgt gatattttag gaattggagt
     1201 tggaacctac tatcataagc tggagtggct gtataggcgt tgtttggagt ttttagaacg
     1261 atatgagaca caaccccttc aaacaaagaa atttaacgag atgtggctta atacggacaa
     1321 gatgcactac tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga aatatacggg
     1381 gtttgaagac cttaatatgc aaacgtatat agttgtatct gctgaggtct tatcaagata
     1441 tgtgttccgt tctgatgttg cctatgattg gaatatttca atggatgaat taaatgaaga
     1501 tacaagaaaa ttcaaagaag accatctcaa tacttttagt cgtaaaaatg ataggttaga
     1561 ttggtcttat tatccacaag aaccatccgc taacgatagc gaaaatagga atgcttatct
     1621 tcacgaatta gggaaaatta ctaataggag tagatttgtc gatggtttaa atgttgatgc
     1681 tccttatact acaactgctc actattggtt aattaaacaa atggttaatg ccgacgaatg
     1741 gcgaatgatt agtgatgatg acttctctat tagaaacgcc ttttatagag tattcacgaa
     1801 agaattaagg ttgagcgatg cacatcattt tatttgccaa gttaacaaga cgaaaagcag
     1861 aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt ggggagatat
     1921 cagaggattc aaaacaaagt ttctacggac tttggcatct cattatctaa ctgaattact
     1981 aacaagtcat caattccacg aagaggctat tagtaaagat ggagaaagat atcgcaaata
     2041 tgccgataac cctatcaaac atccattagc cactaaggat aaaggatttt attcagttga
     2101 ttgtagaacg gatttatctg ctctggaacc aaacgaaatc gcaaagatgc tattaaatgt
     2161 taatgaccat tcgaccaata gctttattca acagataaga cgttatatat catctcttga
     2221 aagaccatta acaacagctc gtggtgataa gaagagctat atttacgcta acttcaatcc
     2281 gaagtatgct caatttgcga ttacaattct aagaacttac tacaactttt gtcgcccttt
     2341 taaatcagcg gataaaaagg ttttgacacc tgctcaaaga ttagggatta cagataaaca
     2401 atttgattgg aaagatataa tttatttcaa atgacaaaaa gagcttggag taatcccaag
     2461 ctcttgtgct ttatctttac tgttcttctt caattaacgc acccgttaac tcaataagtt
     2521 ttttctttca tatacatgaa aatagtagtt atacggattt ttatcattct ttatcccttt
     2581 ttgggcaaat acctcattcc attcctcata attcacttct ggaaaaaaag tatctccttc
     2641 gaattcatga tgtatttttg tgatgtacat tttctcaaca taagggaaaa acaaattata
     2701 aatctgttct cctccgaaaa taaaaatttc ttcttcgttt ttacataact caaaaacatc
     2761 ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat
     2821 aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat
     2881 tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg
     2941 aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt
     3001 catttataac acagcctttt taactaattt accatctaat ttcttatttc tatcaaaaaa
     3061 ccttttctca ttatcctgcc cgttagttga ataagcaatt ttttctataa gttatattat
     3121 cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc attttataaa
     3181 tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta
     3241 cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag
     3301 tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac
     3361 agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga
     3421 aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact
     3481 attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc
     3541 gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga
     3601 tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg
     3661 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     3721 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     3781 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     3841 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     3901 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     3961 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     4021 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     4081 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     4141 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     4201 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     4261 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     4321 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     4381 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     4441 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     4501 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     4561 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     4621 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     4681 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     4741 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     4801 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     4861 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     4921 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     4981 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     5041 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     5101 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     5161 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     5221 agaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     5281 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     5341 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     5401 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     5461 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     5521 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     5581 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     5641 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     5701 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     5761 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     5821 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     5881 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     5941 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     6001 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     6061 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     6121 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     6181 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     6241 ggtttttttg tttctttttt ct
//
LOCUS       DAFCLG010000307         4640 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.155, whole genome shotgun sequence.
ACCESSION   DAFCLG010000307 DAFCLG010000000
VERSION     DAFCLG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.155"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            239..475
                     /locus_tag="LND56_002887"
     CDS             239..475
                     /locus_tag="LND56_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744729.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            482..2434
                     /locus_tag="LND56_002888"
     CDS             482..2434
                     /locus_tag="LND56_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6744730.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2506..3003)
                     /gene="dfrG"
                     /locus_tag="LND56_002889"
     CDS             complement(2506..3003)
                     /gene="dfrG"
                     /locus_tag="LND56_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6744731.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3365..4405
                     /locus_tag="LND56_002890"
     CDS             3365..4405
                     /locus_tag="LND56_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6744732.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4503..>4640
                     /locus_tag="LND56_002891"
     CDS             4503..>4640
                     /locus_tag="LND56_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6744733.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QK"
BASE COUNT         1622 a          741 c          832 g         1445 t
ORIGIN      
        1 ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta aacctaaatg agtatcaaat
       61 tgcaactgaa tttgatactc gttttttatt gttaaatcaa cgtttgttgt taatttacac
      121 cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt gaaataatta ttgtaggaat
      181 gcaaattggt gtgttaaaat ggttacaatt tactaagcaa aaaggggtgc attttcctat
      241 gcaatttctt gagccaaaaa ataagaacgc taagtctgtg gattgggaaa tatcagagca
      301 agtaagggtg atagttaagc aatacgctga atatgcagag cgtactgaaa gcgaagcagt
      361 ggatgaattc ttattgaaca ttttagacga taagaagttt attgaatgga ttgccaacaa
      421 gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa gatagagtgg gttgatagac
      481 aatgaccaag cttaaaagat tagccacgaa ggaagatgaa attgtagatg ttaaaattcc
      541 gatttcaaac gaagaattaa aagatagggc taaacaatat gatttattaa ctccaaaacg
      601 atttgcaaca aggtataaca aaatgttgtt cttgccaacg tcttttaaat ggaacggttc
      661 tgaatatccc attcaatata attactgcat caatccgttt tgttgtaatt ttgggaaaga
      721 acagcacaaa ttcaaagatg taaaagggaa accaagtcga tataaaatga ctggtagttc
      781 caaagataaa ggtcataaag gtatgtactg caatgacaat cctattggca gaggtgtatc
      841 gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt gttgaagaaa ttaaacgcct
      901 tatagaaatc aatagcatcc aagatgttga gccagattat caatttcata aagaaggctg
      961 ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa ttttacaaaa gggggaaaag
     1021 taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag aagttcacta atgttctacc
     1081 aaaaagagaa gaaaccacta cttatcacca acaaaagaac accattcttc caatgttcgc
     1141 taagatggtt gttggaaggg tttcagtcag tcgtacttgt gatattttag gaattggagt
     1201 tggaacctac tatcataagc tggagtggct gtataggcgt tgtttggagt ttttagaacg
     1261 atatgagaca caaccccttc aaacaaagaa atttaacgag atgtggctta atacggacaa
     1321 gatgcactac tacctaaata acgtccgtaa gaaaggacaa ggttcaaaga aatatacggg
     1381 gtttgaagac cttaatatgc aaacgtatat agttgtatct gctgaggtct tatcaagata
     1441 tgtgttccgt tctgatgttg cctatgattg gaatatttca atggatgaat taaatgaaga
     1501 tacaagaaaa ttcaaagaag accatctcaa tacttttagt cgtaaaaatg ataggttaga
     1561 ttggtcttat tatccacaag aaccatccgc taacgatagc gaaaatagga atgcttatct
     1621 tcacgaatta gggaaaatta ctaataggag tagatttgtc gatggtttaa atgttgatgc
     1681 tccttatact acaactgctc actattggtt aattaaacaa atggttaatg ccgacgaatg
     1741 gcgaatgatt agtgatgatg acttctctat tagaaacgcc ttttatagag tattcacgaa
     1801 agaattaagg ttgagcgatg cacatcattt tatttgccaa gttaacaaga cgaaaagcag
     1861 aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa ctgctcgatt ggggagatat
     1921 cagaggattc aaaacaaagt ttctacggac tttggcatct cattatctaa ctgaattact
     1981 aacaagtcat caattccacg aagaggctat tagtaaagat ggagaaagat atcgcaaata
     2041 tgccgataac cctatcaaac atccattagc cactaaggat aaaggatttt attcagttga
     2101 ttgtagaacg gatttatctg ctctggaacc aaacgaaatc gcaaagatgc tattaaatgt
     2161 taatgaccat tcgaccaata gctttattca acagataaga cgttatatat catctcttga
     2221 aagaccatta acaacagctc gtggtgataa gaagagctat atttacgcta acttcaatcc
     2281 gaagtatgct caatttgcga ttacaattct aagaacttac tacaactttt gtcgcccttt
     2341 taaatcagcg gataaaaagg ttttgacacc tgctcaaaga ttagggatta cagataaaca
     2401 atttgattgg aaagatataa tttatttcaa atgacaaaaa gagcttggag taatcccaag
     2461 ctcttgtgct ttatctttac tgttcttctt caattaacgc acccgttaac tcaataagtt
     2521 ttttctttca tatacatgaa aatagtagtt atacggattt ttatcattct ttatcccttt
     2581 ttgggcaaat acctcattcc attcctcata attcacttct ggaaaaaaag tatctccttc
     2641 gaattcatga tgtatttttg tgatgtacat tttctcaaca taagggaaaa acaaattata
     2701 aatctgttct cctccgaaaa taaaaatttc ttcttcgttt ttacataact caaaaacatc
     2761 ttctattgaa tgaacaattt cacaaccatt aaaggtaaac cccttatctc tcgtcagaat
     2821 aatatttctt ctgtcaggta aggctcttcc gattgattca aggttcttcc tacctaatat
     2881 tatcggatgt ccctttgtag tatttttaac atattcccag tccttgggaa tcctccaagg
     2941 aatgtcattc tctttgccaa tcactctatt cttatccatc gcagcaatca aagaaacttt
     3001 catttataac acagcctttt taactaattt accatctaat ttcttatttc tatcaaaaaa
     3061 ccttttctca ttatcctgcc cgttagttga ataagcaatt ttttctataa gttatattat
     3121 cataatacca caaaatcaca aattttcatt caattatcat tttcaattgc attttataaa
     3181 tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa aagtagtcca ttttgaagta
     3241 cattttgtca ttttgtactc caaaactgaa ctacttactt gcatatatac gcctttatag
     3301 tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac
     3361 agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga
     3421 aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact
     3481 attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc
     3541 gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga
     3601 tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg
     3661 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     3721 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     3781 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     3841 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     3901 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     3961 gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc ctgttagtac
     4021 tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta gaaaagctgg
     4081 aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg aatatctttc
     4141 acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc aactgaaaaa
     4201 taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt atcgcaatga
     4261 tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag gatatcaggt
     4321 taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag ataaagtaag
     4381 atatatgttc aataaaataa cttagtgcgt tttatgttgt tatataaata tggtttctta
     4441 ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag gaggagattg
     4501 ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca agactacgaa
     4561 tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt aagaacgaaa
     4621 ctttaattaa tgttcaaaaa
//
LOCUS       DAFCLG010000308         3798 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.173, whole genome shotgun sequence.
ACCESSION   DAFCLG010000308 DAFCLG010000000
VERSION     DAFCLG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.173"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002892"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK6744734.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="LND56_002893"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="LND56_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK6744735.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            complement(3461..3661)
                     /locus_tag="LND56_002894"
     CDS             complement(3461..3661)
                     /locus_tag="LND56_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744736.1"
                     /translation="MPIFILSVLSSLPKARLSKNKIFIVFRFSSVTDKTTQNKKDTRE
                     VSRIFYSAIAPFNGTKRFEFLL"
BASE COUNT         1200 a          583 c          776 g         1239 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttct
//
LOCUS       DAFCLG010000309         3798 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.174, whole genome shotgun sequence.
ACCESSION   DAFCLG010000309 DAFCLG010000000
VERSION     DAFCLG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.174"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002895"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK6744737.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="LND56_002896"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="LND56_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBK6744738.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            complement(3461..3661)
                     /locus_tag="LND56_002897"
     CDS             complement(3461..3661)
                     /locus_tag="LND56_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6744739.1"
                     /translation="MPIFILSVLSSLPKARLSKNKIFIVFRFSSVTDKTTQNKKDTRE
                     VSRIFYSAIAPFNGTKRFEFLL"
BASE COUNT         1199 a          583 c          777 g         1239 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttct
//
LOCUS       DAFCLG010000310         3729 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.176, whole genome shotgun sequence.
ACCESSION   DAFCLG010000310 DAFCLG010000000
VERSION     DAFCLG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.176"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..484)
                     /locus_tag="LND56_002898"
     CDS             complement(<1..484)
                     /locus_tag="LND56_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6744740.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     T"
     gene            610..1731
                     /locus_tag="LND56_002899"
     CDS             610..1731
                     /locus_tag="LND56_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6744741.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1989..3491
                     /gene="eat(A)"
                     /locus_tag="LND56_002900"
     CDS             1989..3491
                     /gene="eat(A)"
                     /locus_tag="LND56_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6744742.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3558..>3729
                     /locus_tag="LND56_002901"
     CDS             3558..>3729
                     /locus_tag="LND56_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6744743.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQ"
BASE COUNT         1250 a          676 c          806 g          997 t
ORIGIN      
        1 ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca
       61 atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat
      121 ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca
      181 aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat
      241 tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg
      301 gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat
      361 ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc
      421 tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt
      481 tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga
      541 aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga
      601 gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt
      661 gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg
      721 ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca
      781 tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg
      841 acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat
      901 ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa
      961 cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc
     1021 gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac
     1081 acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt
     1141 gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa
     1201 ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta
     1261 gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat
     1321 gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg
     1381 aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa
     1441 caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa
     1501 gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc
     1561 ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt
     1621 catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca
     1681 aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa
     1741 tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg
     1801 aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc
     1861 agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat
     1921 ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt
     1981 gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca
     2041 cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg
     2101 gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc
     2161 aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag
     2221 aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa
     2281 gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat
     2341 cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc
     2401 cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt
     2461 atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg
     2521 aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt
     2581 tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa
     2641 aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc
     2701 gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac
     2761 gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa
     2821 tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat
     2881 atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag
     2941 aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc
     3001 ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt
     3061 atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga
     3121 gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag
     3181 agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa
     3241 gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat
     3301 tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt
     3361 tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt
     3421 tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg
     3481 agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca
     3541 acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac
     3601 aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac
     3661 agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc
     3721 ctcttcaac
//
LOCUS       DAFCLG010000311         2871 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.194, whole genome shotgun sequence.
ACCESSION   DAFCLG010000311 DAFCLG010000000
VERSION     DAFCLG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.194"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..143
                     /locus_tag="LND56_002902"
     CDS             <1..143
                     /locus_tag="LND56_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6744744.1"
                     /translation="DTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGT
                     KK"
     gene            127..756
                     /locus_tag="LND56_002903"
     CDS             127..756
                     /locus_tag="LND56_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6744745.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1110..2588
                     /gene="msr(C)"
                     /locus_tag="LND56_002904"
     CDS             1110..2588
                     /gene="msr(C)"
                     /locus_tag="LND56_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6744746.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2635..>2871
                     /locus_tag="LND56_002905"
     CDS             2635..>2871
                     /locus_tag="LND56_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6744747.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQM"
BASE COUNT          991 a          474 c          608 g          798 t
ORIGIN      
        1 aagatacagg ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa caatttctaa
       61 cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg aaagaggtta
      121 ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag atccgttcgc
      181 gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat caaagaagcg
      241 acgactgcac cttcttctgt caatatgcaa ccatggcgct ttgtggtcgc tgaaagcgag
      301 gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa tgatacttct
      361 tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga agaaatctat
      421 aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca attagctgcg
      481 atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt caaagtcgat
      541 tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta tgaaacaaat
      601 ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga taaagaccgt
      661 tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta cgagtctgtt
      721 cgtttagcac cagaaaagat cactacgttc gaataaaagg ttttcagtta ctattcagta
      781 gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga
      841 ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca
      901 tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac agtagaatag
      961 gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga gtaggaaaag
     1021 atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc gataattgtt
     1081 gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat ataacaaatc
     1141 tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt gtctatcagc
     1201 aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa
     1261 tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc ttcaactacc
     1321 tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta gccagtcatt
     1381 tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa gaacgaaaga
     1441 tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac gagccaacaa
     1501 cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat tattatggta
     1561 cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg aaaatctggg
     1621 aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat ctcactcaaa
     1681 aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa gagaaaaaac
     1741 gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca
     1801 gtaaaaagaa aaagcaacaa atcagaccgg atcggttgtc ttcctctaaa caaaaagaca
     1861 gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc caaaaaatag
     1921 gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca aaatctcttg
     1981 aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga agtgggagaa
     2041 cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg
     2101 aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga gaaggaatcc
     2161 ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa atgacatctg
     2221 aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg cttgtccgta
     2281 gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta tgtacattaa
     2341 gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca agtaatgtgt
     2401 tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct ttagagaagc
     2461 taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac tttgtcgagc
     2521 gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa
     2581 atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta cagattgcct
     2641 acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat ggtcttgggg
     2701 atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt gttgattggt
     2761 ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg
     2821 aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat g
//
LOCUS       DAFCLG010000312         2552 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.196, whole genome shotgun sequence.
ACCESSION   DAFCLG010000312 DAFCLG010000000
VERSION     DAFCLG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.196"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..459
                     /locus_tag="LND56_002906"
     CDS             <1..459
                     /locus_tag="LND56_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6744748.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="LND56_002907"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="LND56_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6744749.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="LND56_002908"
     CDS             1346..2500
                     /locus_tag="LND56_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6744750.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVQITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          454 c          535 g          683 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg caaatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata tattttggct
     2521 ctttgtcaaa tgatgttggt aaagagaaat ct
//
LOCUS       DAFCLG010000313         2176 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.204, whole genome shotgun sequence.
ACCESSION   DAFCLG010000313 DAFCLG010000000
VERSION     DAFCLG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.204"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002909"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK6744751.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..>2176
                     /locus_tag="LND56_002910"
     CDS             2039..>2176
                     /locus_tag="LND56_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6744752.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QK"
BASE COUNT          758 a          331 c          441 g          646 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaa
//
LOCUS       DAFCLG010000314         2176 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.205, whole genome shotgun sequence.
ACCESSION   DAFCLG010000314 DAFCLG010000000
VERSION     DAFCLG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.205"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002911"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="LND56_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK6744753.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..>2176
                     /locus_tag="LND56_002912"
     CDS             2039..>2176
                     /locus_tag="LND56_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6744754.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QK"
BASE COUNT          757 a          331 c          442 g          646 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaa
//
LOCUS       DAFCLG010000315         1570 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.221, whole genome shotgun sequence.
ACCESSION   DAFCLG010000315 DAFCLG010000000
VERSION     DAFCLG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.221"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            266..1141
                     /locus_tag="LND56_002913"
                     /note="aph(2'')-Ia"
     CDS             266..1141
                     /locus_tag="LND56_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBK6744755.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1271..>1570)
                     /locus_tag="LND56_002914"
     CDS             complement(1271..>1570)
                     /locus_tag="LND56_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6744756.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          622 a          150 c          264 g          534 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
     1141 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
     1201 gaaaaataat aaaggaagtg agtcaattcc agactcctgt gtaaaatgct atacaatgtt
     1261 tttaccattt ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat
     1321 gtaggtccat aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc
     1381 gaataatctt ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct
     1441 ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat
     1501 ccaatgatgc gcaagctttt gaatattttg gttggttctg ttgcaaagtt ttaaatctac
     1561 tatcaaataa
//
LOCUS       DAFCLG010000316         1044 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.245, whole genome shotgun sequence.
ACCESSION   DAFCLG010000316 DAFCLG010000000
VERSION     DAFCLG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.245"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="LND56_002915"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="LND56_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HBK6744757.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            206..940
                     /locus_tag="LND56_002916"
     CDS             206..940
                     /locus_tag="LND56_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HBK6744758.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          420 a          138 c          140 g          346 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacattcat
      181 tataacctca taggagtggg ttataatgaa caaaaaaaat ataaaagata gtcaaaactt
      241 tattacttca aagcatcata taaatgaaat tttgagaaat gtacatttaa atacaaatga
      301 taatattatt gagattggtt cagggaaagg tcatttcacg tttgaattag ctaaaaggtg
      361 taattatgta accgccattg aaatagatcc taaattatgt aggataacta aaaacaaact
      421 tattgaatat gagaacttcc aggttatcaa taaagatatt ttacaattta agtttcctaa
      481 aaataagtca tataagatat atggaaatat accctacaat ataagtacag atataattcg
      541 aaaaattgtt tttgaaagca cagctacaga aagttattta atagtggaat atggatttgc
      601 taaaaggttg ctaaatacaa atcgttcact agcactattt ttaatgacag aagttgatat
      661 atccatatta agtaaaatcc ctagagaata ctttcatcca aaacctagag ttaatagctc
      721 gttaattgta ttaaaaagac acccttcaaa aatatcactc aaagatagaa aacaatatga
      781 aaattttgtt atgaaatggg ttaacaaaga atacataaaa ctattttcca aaaaccaatt
      841 ttatcaagcc ttaaaatatg caagaattga cgatttaaac aatattagct ttgaacaatt
      901 cttgtctctt ttcaatagct ataaattatt taatagataa gttaaggggt gcataaactg
      961 catcccttaa cttatcttat tttgcttaaa caatcttggc ccgtaaactg cataactcat
     1021 atgtaaatcg ctccttttta ggtg
//
LOCUS       DAFCLG010000317          957 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V2381
            SAMN22631149-rid13289993.guided.249, whole genome shotgun sequence.
ACCESSION   DAFCLG010000317 DAFCLG010000000
VERSION     DAFCLG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631149
            Sequence Read Archive: SRR16608728
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171232.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171232.1
            Genome Coverage       :: 43x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:55:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,917
            CDSs (total)                      :: 2,857
            Genes (coding)                    :: 2,800
            CDSs (with protein)               :: 2,800
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 32 of 57
            Pseudo Genes (incomplete)         :: 18 of 57
            Pseudo Genes (internal stop)      :: 15 of 57
            Pseudo Genes (multiple problems)  :: 8 of 57
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631149-rid13289993.guided.249"
                     /isolate="V2381"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-01-30"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            242..790
                     /gene="aac(6')"
                     /locus_tag="LND56_002917"
     CDS             242..790
                     /gene="aac(6')"
                     /locus_tag="LND56_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6744759.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          337 a          142 c          202 g          276 t
ORIGIN      
        1 tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat
       61 tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt
      121 tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag
      181 cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg
      241 tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt
      301 actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat
      361 gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat
      421 tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag
      481 ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc
      541 ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt
      601 aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg
      661 tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa
      721 tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa
      781 ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat
      841 ttacgaaaat tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt
      901 ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaa
//
